L H J Looijenga


Affiliation: Erasmus MC
Country: The Netherlands


  1. Dorssers L, Gillis A, Stoop H, van Marion R, Nieboer M, van Riet J, et al. Molecular heterogeneity and early metastatic clone selection in testicular germ cell cancer development. Br J Cancer. 2019;120:444-452 pubmed publisher
    ..These observations of heterogeneity at all stages of tumourigenesis should be considered when treating patients with GCNIS-only disease, or with clinically overt NS. ..
  2. Salvatori D, Dorssers L, Gillis A, Perretta G, van Agthoven T, Gomes Fernandes M, et al. The MicroRNA-371 Family as Plasma Biomarkers for Monitoring Undifferentiated and Potentially Malignant Human Pluripotent Stem Cells in Teratoma Assays. Stem Cell Reports. 2018;11:1493-1505 pubmed publisher
    ..miR-371 family members were also identified in mouse plasma faithfully reporting undifferentiated elements in the xenografts. This study demonstrated that undifferentiated and potentially malignant cells could be detected in vivo. ..
  3. Rijlaarsdam M, Rijlaarsdam D, Gillis A, Dorssers L, Looijenga L. miMsg: a target enrichment algorithm for predicted miR-mRNA interactions based on relative ranking of matched expression data. Bioinformatics. 2013;29:1638-46 pubmed publisher
    ..Matlab source code and datasets available at www.martinrijlaarsdam.nl/mimsg . Supplementary data are available at Bioinformatics online. ..
  4. Looijenga L, Stoop H, Biermann K. Testicular cancer: biology and biomarkers. Virchows Arch. 2014;464:301-13 pubmed publisher
    ..The latter would allow screening of defined populations, early diagnosis, optimal follow-up, and potentially early treatment, preventing long-term side effects of systemic treatment. ..
  5. van der Zwan Y, Biermann K, Wolffenbuttel K, Cools M, Looijenga L. Gonadal maldevelopment as risk factor for germ cell cancer: towards a clinical decision model. Eur Urol. 2015;67:692-701 pubmed publisher
    ..A combined effect of epigenetic and environmental factors is identified in the pathogenesis, and a model is proposed to apply this knowledge to clinical practice. ..
  6. Leao R, van Agthoven T, Figueiredo A, Jewett M, Fadaak K, Sweet J, et al. Serum miRNA Predicts Viable Disease after Chemotherapy in Patients with Testicular Nonseminoma Germ Cell Tumor. J Urol. 2018;200:126-135 pubmed publisher
    ..02). Our study demonstrates for the first time the potential value of miR-371a-3p to predict viable germ cell tumors in residual masses after chemotherapy. Prospective studies are required to confirm clinical usefulness. ..
  7. Spiller C, Gillis A, Burnet G, Stoop H, Koopman P, Bowles J, et al. Cripto: Expression, epigenetic regulation and potential diagnostic use in testicular germ cell tumors. Mol Oncol. 2016;10:526-37 pubmed publisher
    ..These findings suggest that CRIPTO expression may be a useful serological marker for diagnostic and/or prognostic purposes during germ cell cancer management. ..
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    Looijenga L, Stoop H, de Leeuw H, de Gouveia Brazao C, Gillis A, van Roozendaal K, et al. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res. 2003;63:2244-50 pubmed
    ..Therefore, POU5F1 immunohistochemistry is an informative diagnostic tool for pluripotent GCT and offers new insights into the histological heterogeneity of this cancer. ..
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    Looijenga L, Stoop H, Hersmus R, Gillis A, Wolter Oosterhuis J. Genomic and expression profiling of human spermatocytic seminomas: pathogenetic implications. Int J Androl. 2007;30:328-35; discussion 335-6 pubmed
    ..The canine seminomas are currently considered as the most informative model for human spermatocytic seminomas. ..

More Information


  1. Looijenga L, Gillis A, Stoop H, Biermann K, Oosterhuis J. Dissecting the molecular pathways of (testicular) germ cell tumour pathogenesis; from initiation to treatment-resistance. Int J Androl. 2011;34:e234-51 pubmed publisher
    ..Further understanding will allow development of more targeted treatment, which will benefit quality of life of these young cancer patients. ..
  2. van Agthoven T, Eijkenboom W, Looijenga L. microRNA-371a-3p as informative biomarker for the follow-up of testicular germ cell cancer patients. Cell Oncol (Dordr). 2017;40:379-388 pubmed publisher
    ..The ampTSmiR test is more sensitive than the conventional TGCC protein biomarkers for the detection of residual disease and relapse, excluding mature teratoma. ..
  3. Rijlaarsdam M, van der Zwan Y, Dorssers L, Looijenga L. DMRforPairs: identifying differentially methylated regions between unique samples using array based methylation profiles. BMC Bioinformatics. 2014;15:141 pubmed publisher
    ..To our knowledge, DMRforPairs is the first tool to identify and visualize relevant and significant differentially methylated regions between unique samples. ..
  4. Eckert D, Biermann K, Nettersheim D, Gillis A, Steger K, Jack H, et al. Expression of BLIMP1/PRMT5 and concurrent histone H2A/H4 arginine 3 dimethylation in fetal germ cells, CIS/IGCNU and germ cell tumors. BMC Dev Biol. 2008;8:106 pubmed publisher
    ..This imposes epigenetic modifications leading to transcriptional repression in mouse PGCs enabling them to escape the somatic differentiation program during migration, while expressing markers of pluripotency...
  5. Looijenga L. Spermatocytic seminoma: toward further understanding of pathogenesis. J Pathol. 2011;224:431-3 pubmed publisher
    ..However, this does not exclude an earlier cell of origin, possibly explaining the unique properties of this type of human germ cell tumour, with various counterparts in animals...
  6. Looijenga L, van Agthoven T, Biermann K. Development of malignant germ cells - the genvironmental hypothesis. Int J Dev Biol. 2013;57:241-53 pubmed publisher
    ..These insights will allow a better definition of individuals at risk of developing a germ cell malignancy, and allow a better selection of scientific approaches to elucidate the corresponding pathogenesis...
  7. Looijenga L, Hersmus R, de Leeuw B, Stoop H, Cools M, Oosterhuis J, et al. Gonadal tumours and DSD. Best Pract Res Clin Endocrinol Metab. 2010;24:291-310 pubmed publisher
  8. Looijenga L. Human testicular (non)seminomatous germ cell tumours: the clinical implications of recent pathobiological insights. J Pathol. 2009;218:146-62 pubmed publisher
    ..The availability of representative cell lines, both for seminoma and for embryonal carcinoma, allows mechanistic studies into the initiation and progression of this disease. ..
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    Looijenga L, Gillis A, Stoop H, Hersmus R, Oosterhuis J. Chromosomes and expression in human testicular germ-cell tumors: insight into their cell of origin and pathogenesis. Ann N Y Acad Sci. 2007;1120:187-214 pubmed
    ..The conclusion is that TGCTs are embryonic cancers found in adults. ..
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    Looijenga L, Hersmus R, Oosterhuis J, Cools M, Drop S, Wolffenbuttel K. Tumor risk in disorders of sex development (DSD). Best Pract Res Clin Endocrinol Metab. 2007;21:480-95 pubmed
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    Looijenga L, Gillis A, Stoop H, Hersmus R, Oosterhuis J. Relevance of microRNAs in normal and malignant development, including human testicular germ cell tumours. Int J Androl. 2007;30:304-14; discussion 314-5 pubmed
    ..Specific sets of differentiating miRNA were found to characterize the various differentiation lineages within the GCTs, which simulate normal embryonic development. ..
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    Looijenga L, de Leeuw H, van Oorschot M, van Gurp R, Stoop H, Gillis A, et al. Stem cell factor receptor (c-KIT) codon 816 mutations predict development of bilateral testicular germ-cell tumors. Cancer Res. 2003;63:7674-8 pubmed
    ..These patients may undergo tailored treatment to prevent the development of bilateral disease, with retention of testicular hormonal function. ..