L H J Looijenga

Summary

Affiliation: Erasmus MC
Country: The Netherlands

Publications

  1. pmc DMRforPairs: identifying differentially methylated regions between unique samples using array based methylation profiles
    Martin A Rijlaarsdam
    Department of Pathology, Laboratory for Experimental Patho Oncology, Erasmus MC University Medical Center Rotterdam, Be 432, P, O, Box 2040, Rotterdam 3000 CA, Netherlands
    BMC Bioinformatics 15:141. 2014
  2. pmc Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours
    M A Rijlaarsdam
    Department of Pathology, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Br J Cancer 105:854-63. 2011
  3. pmc Delayed Recognition of Disorders of Sex Development (DSD): A Missed Opportunity for Early Diagnosis of Malignant Germ Cell Tumors
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Int J Endocrinol 2012:671209. 2012
  4. pmc Prevalence of c-KIT mutations in gonadoblastoma and dysgerminomas of patients with disorders of sex development (DSD) and ovarian dysgerminomas
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    PLoS ONE 7:e43952. 2012
  5. pmc A 46,XY female DSD patient with bilateral gonadoblastoma, a novel SRY missense mutation combined with a WT1 KTS splice-site mutation
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Rotterdam, The Netherlands
    PLoS ONE 7:e40858. 2012
  6. pmc Role of SOX2 in the etiology of embryonal carcinoma, based on analysis of the NCCIT and NT2 cell lines
    Ronak Eini
    Erasmus MC, University Medical Center Rotterdam, Department of Pathology, Rotterdam, The Netherlands
    PLoS ONE 9:e83585. 2014
  7. ncbi request reprint Testicular cancer: biology and biomarkers
    Leendert H J Looijenga
    Department of Pathology, Laboratory of Experimental Patho Oncology, Erasmus MC University Medical Center Rotterdam, P O Box 2040, 3000, CA, Rotterdam, The Netherlands
    Virchows Arch 464:301-13. 2014
  8. pmc DICER1 RNase IIIb domain mutations are infrequent in testicular germ cell tumours
    Carmela M de Boer
    Center for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Australia
    BMC Res Notes 5:569. 2012
  9. ncbi request reprint miMsg: a target enrichment algorithm for predicted miR-mRNA interactions based on relative ranking of matched expression data
    Martin A Rijlaarsdam
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Bioinformatics 29:1638-46. 2013
  10. doi request reprint Development of malignant germ cells - the genvironmental hypothesis
    Leendert H J Looijenga
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Int J Dev Biol 57:241-53. 2013

Detail Information

Publications97

  1. pmc DMRforPairs: identifying differentially methylated regions between unique samples using array based methylation profiles
    Martin A Rijlaarsdam
    Department of Pathology, Laboratory for Experimental Patho Oncology, Erasmus MC University Medical Center Rotterdam, Be 432, P, O, Box 2040, Rotterdam 3000 CA, Netherlands
    BMC Bioinformatics 15:141. 2014
    ..These tools all require groups of samples to compare. However, comparison of unique, individual samples is essential in situations where larger sample sizes are not possible...
  2. pmc Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours
    M A Rijlaarsdam
    Department of Pathology, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Br J Cancer 105:854-63. 2011
    ..Of the three identified isoforms--OCT4A, OCT4B and OCT4B1--only OCT4A proved to regulate pluripotency. Up until now, no convincing nuclear OCT4A protein expression has been shown in somatic cancers or tissues...
  3. pmc Delayed Recognition of Disorders of Sex Development (DSD): A Missed Opportunity for Early Diagnosis of Malignant Germ Cell Tumors
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Int J Endocrinol 2012:671209. 2012
    ..These cases demonstrate overlap between DSD and the so-called testicular dysgenesis syndrome (TDS), of significant relevance for identification of individuals at increased risk for development of a malignant GCT...
  4. pmc Prevalence of c-KIT mutations in gonadoblastoma and dysgerminomas of patients with disorders of sex development (DSD) and ovarian dysgerminomas
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    PLoS ONE 7:e43952. 2012
    ..This data supports the existence of two distinct but parallel pathways in the development of dysgerminoma, in which mutational status of c-KIT might parallel the presence of TSPY...
  5. pmc A 46,XY female DSD patient with bilateral gonadoblastoma, a novel SRY missense mutation combined with a WT1 KTS splice-site mutation
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Rotterdam, The Netherlands
    PLoS ONE 7:e40858. 2012
    ..The case presented shows the importance of multi-gene based diagnosis of DSD patients, allowing early diagnosis and treatment, thus preventing putative development of an invasive cancer...
  6. pmc Role of SOX2 in the etiology of embryonal carcinoma, based on analysis of the NCCIT and NT2 cell lines
    Ronak Eini
    Erasmus MC, University Medical Center Rotterdam, Department of Pathology, Rotterdam, The Netherlands
    PLoS ONE 9:e83585. 2014
    ..This finding is likely related to the presence of wild type p53 in GCC, resulting in expression of downstream target genes, amongst others miR-34a, miR-145 and SOX2, associated to the unique sensitivity of GCC to DNA damaging agents. ..
  7. ncbi request reprint Testicular cancer: biology and biomarkers
    Leendert H J Looijenga
    Department of Pathology, Laboratory of Experimental Patho Oncology, Erasmus MC University Medical Center Rotterdam, P O Box 2040, 3000, CA, Rotterdam, The Netherlands
    Virchows Arch 464:301-13. 2014
    ..The latter would allow screening of defined populations, early diagnosis, optimal follow-up, and potentially early treatment, preventing long-term side effects of systemic treatment...
  8. pmc DICER1 RNase IIIb domain mutations are infrequent in testicular germ cell tumours
    Carmela M de Boer
    Center for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Australia
    BMC Res Notes 5:569. 2012
    ..Additional analysis of 14 TGCT DNA samples identified one case that also contained a mutation at one of these sites...
  9. ncbi request reprint miMsg: a target enrichment algorithm for predicted miR-mRNA interactions based on relative ranking of matched expression data
    Martin A Rijlaarsdam
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Bioinformatics 29:1638-46. 2013
    ..It is desirable to develop a transparent, user-friendly, unbiased tool to enrich miR-mRNA predictions...
  10. doi request reprint Development of malignant germ cells - the genvironmental hypothesis
    Leendert H J Looijenga
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Int J Dev Biol 57:241-53. 2013
    ..These insights will allow a better definition of individuals at risk of developing a germ cell malignancy, and allow a better selection of scientific approaches to elucidate the corresponding pathogenesis...
  11. pmc SRY mutation analysis by next generation (deep) sequencing in a cohort of chromosomal Disorders of Sex Development (DSD) patients with a mosaic karyotype
    Remko Hersmus
    Department of Pathology, Erasmus MC, University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    BMC Med Genet 13:108. 2012
    ..SRY mutations have also been found in a small number of patients with a 45,X/46,XY karyotype and might play a role in the maldevelopment of the gonads...
  12. pmc A SRY-HMG box frame shift mutation inherited from a mosaic father with a mild form of testicular dysgenesis syndrome in Turner syndrome patient
    Mohammad Shahid
    College of Dentistry, Alkharj University, Alkharj, Kingdom of Saudi Arabia
    BMC Med Genet 11:131. 2010
    ..The majority of mutations within the SRY gene are de novo affecting only a single individual in the family. The mutations within the high-mobility group (HMG) region have the potential to affect its DNA binding activity...
  13. pmc Expression of BLIMP1/PRMT5 and concurrent histone H2A/H4 arginine 3 dimethylation in fetal germ cells, CIS/IGCNU and germ cell tumors
    Dawid Eckert
    Department of Developmental Pathology, Institute of Pathology, University of Bonn, Germany
    BMC Dev Biol 8:106. 2008
    ..This imposes epigenetic modifications leading to transcriptional repression in mouse PGCs enabling them to escape the somatic differentiation program during migration, while expressing markers of pluripotency...
  14. doi request reprint Human testicular (non)seminomatous germ cell tumours: the clinical implications of recent pathobiological insights
    Leendert H J Looijenga
    Department of Pathology, Erasmus MC Erasmus University Medical Center, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    J Pathol 218:146-62. 2009
    ..The availability of representative cell lines, both for seminoma and for embryonal carcinoma, allows mechanistic studies into the initiation and progression of this disease...
  15. doi request reprint Dissecting the molecular pathways of (testicular) germ cell tumour pathogenesis; from initiation to treatment-resistance
    L H J Looijenga
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Int J Androl 34:e234-51. 2011
    ..Further understanding will allow development of more targeted treatment, which will benefit quality of life of these young cancer patients...
  16. doi request reprint Spermatocytic seminoma: toward further understanding of pathogenesis
    Leendert Hj Looijenga
    Department of Pathology, Erasmus MC University Medical Centre Rotterdam, The Netherlands
    J Pathol 224:431-3. 2011
    ..However, this does not exclude an earlier cell of origin, possibly explaining the unique properties of this type of human germ cell tumour, with various counterparts in animals...
  17. ncbi request reprint Genomic and expression profiling of human spermatocytic seminomas: pathogenetic implications
    Leendert H J Looijenga
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Int J Androl 30:328-35; discussion 335-6. 2007
    ..The canine seminomas are currently considered as the most informative model for human spermatocytic seminomas...
  18. doi request reprint [Advances in basic research on testicular germ cell tumors : clinical implications]
    L H J Looijenga
    Department of Pathology, Erasmus MC Erasmus University Medical Center, Josephine Nefkens Institute, 2040, NL 3000, CA Rotterdam, Niederlande
    Urologe A 48:350-8. 2009
    ..Micro-satellite instability as well as BRAF mutations have been identified to be related to treatment resistance, possibly leading to improved clinical management...
  19. ncbi request reprint Gonadal tumours and DSD
    Leendert H J Looijenga
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Best Pract Res Clin Endocrinol Metab 24:291-310. 2010
    ....
  20. ncbi request reprint Clinical value of the X chromosome in testicular germ-cell tumours
    Leendert H J Looijenga
    Department of Pathology, Laboratory for Experimental Patho Oncology, Erasmus MC Erasmus University Medical Center Rotterdam Daniel den Hoed Cancer Center, 3000 DR, Rotterdam, Netherlands
    Lancet 363:6-8. 2004
  21. ncbi request reprint Genomic and expression profiling of human spermatocytic seminomas: primary spermatocyte as tumorigenic precursor and DMRT1 as candidate chromosome 9 gene
    Leendert H J Looijenga
    Department of Pathology, Josephine Nefkens Institute, Erasmus Medical Center University Medical Center, Rotterdam, The Netherlands
    Cancer Res 66:290-302. 2006
    ....
  22. ncbi request reprint POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors
    Leendert H J Looijenga
    Pathology Lab for Exp Patho Oncology, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Cancer Res 63:2244-50. 2003
    ..Therefore, POU5F1 immunohistochemistry is an informative diagnostic tool for pluripotent GCT and offers new insights into the histological heterogeneity of this cancer...
  23. ncbi request reprint Tumor risk in disorders of sex development (DSD)
    Leendert H J Looijenga
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Building Be, Room 430b, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Best Pract Res Clin Endocrinol Metab 21:480-95. 2007
    ....
  24. ncbi request reprint Chromosomes and expression in human testicular germ-cell tumors: insight into their cell of origin and pathogenesis
    Leendert H J Looijenga
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Ann N Y Acad Sci 1120:187-214. 2007
    ..The conclusion is that TGCTs are embryonic cancers found in adults...
  25. ncbi request reprint Relevance of microRNAs in normal and malignant development, including human testicular germ cell tumours
    L H J Looijenga
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Int J Androl 30:304-14; discussion 314-5. 2007
    ..Specific sets of differentiating miRNA were found to characterize the various differentiation lineages within the GCTs, which simulate normal embryonic development...
  26. ncbi request reprint Stem cell factor receptor (c-KIT) codon 816 mutations predict development of bilateral testicular germ-cell tumors
    Leendert H J Looijenga
    Pathology Laboratory for Experimental Patho Oncology, Erasmus MC, University Medical Center Rotterdam, den Hoed Cancer Center, Nefkens Institute, Building Be, Room 430b, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Cancer Res 63:7674-8. 2003
    ..These patients may undergo tailored treatment to prevent the development of bilateral disease, with retention of testicular hormonal function...
  27. doi request reprint Differential expression of SOX17 and SOX2 in germ cells and stem cells has biological and clinical implications
    J De Jong
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Pathol 215:21-30. 2008
    ..It allows the identification of seminoma-like cells from embryonal carcinoma based on a positive marker and might be the explanation for the different function of POU5F1 in normal and malignant germ cells versus embryonic stem cells...
  28. doi request reprint Expression and interdependencies of pluripotency factors LIN28, OCT3/4, NANOG and SOX2 in human testicular germ cells and tumours of the testis
    A J M Gillis
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Int J Androl 34:e160-74. 2011
    ..In conclusion, LIN28 regulates the differentiation status of seminoma and embryonal carcinoma and is likely to play a related role in normal human germ-cell development...
  29. doi request reprint FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD)
    R Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Pathol 215:31-8. 2008
    ..In addition, it initiates a novel way to study the role of the supportive cells in the development of either CIS or GB...
  30. ncbi request reprint High-throughput microRNAome analysis in human germ cell tumours
    A J M Gillis
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed, Rotterdam, The Netherlands
    J Pathol 213:319-28. 2007
    ..Moreover, differentiated non-seminomas showed overexpression of discriminating miRNAs. These results support the model that miRNAs are involved in regulating differentiation of stem cells, retained in GCTs...
  31. ncbi request reprint Stem cell factor as a novel diagnostic marker for early malignant germ cells
    H Stoop
    Department of Pathology, Erasmus MC Erasmus University Medical Center, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    J Pathol 216:43-54. 2008
    ..SCF immunohistochemistry can be a valuable diagnostic tool, in addition to OCT3/4, to screen for precursor lesions of TGCTs, especially in patients with germ cell maturation delay...
  32. doi request reprint Evaluation of testicular biopsies for carcinoma in situ: immunohistochemistry is mandatory
    N J van Casteren
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Int J Androl 32:666-74. 2009
    ..Application of this protocol will allow early diagnosis, and therefore prevent any adverse anti-cancer treatment sequelae including gonadectomy, and requiring life long androgen supplementation in some patients...
  33. ncbi request reprint Identification of germ cells at risk for neoplastic transformation in gonadoblastoma: an immunohistochemical study for OCT3/4 and TSPY
    Anne Marie F Kersemaekers
    Department of Pathology, Josephine Nefkens Institute, Daniel de Hoed Cancer Center, Erasmus MC University Medical Center Rotterdam, The Netherlands
    Hum Pathol 36:512-21. 2005
    ..This could allow better prediction of the risk of progression to a GCT. In addition, the data support the model that GB represents the earliest accessible developmental stage of malignant GCTs...
  34. doi request reprint Testicular microlithiasis and carcinoma in situ overview and proposed clinical guideline
    N J van Casteren
    Department of Urology, Erasmus MC, Rotterdam, The Netherlands
    Int J Androl 32:279-87. 2009
    ..A long-term active follow-up schedule, including ultrasonography and physical examinations, is not indicated in the remaining patients with TM...
  35. ncbi request reprint Bilateral testicular microlithiasis predicts the presence of the precursor of testicular germ cell tumors in subfertile men
    C A De Gouveia Brazao
    Department of Andrology, and Laboratory for Experimental Pathology, Josephine Nefkens Institute, Erasmus Medical Center University Medical Center Rotterdam, The Netherlands
    J Urol 171:158-60. 2004
    ..We determined the predictive value of unilateral and bilateral testicular microlithiasis for CIS in subfertile men, a known risk population for TGCTs (approximately 1%)...
  36. ncbi request reprint Differential methylation of the OCT3/4 upstream region in primary human testicular germ cell tumors
    Jeroen de Jong
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, 3000 CA Rotterdam, The Netherlands
    Oncol Rep 18:127-32. 2007
    ..Moreover, the findings argue against OCT3/4 being a driving oncogenic factor in the pathogenesis of human germ cell tumors...
  37. ncbi request reprint The predictive value of testicular ultrasound abnormalities for carcinoma in situ of the testis in men at risk for testicular cancer
    J E Elzinga-Tinke
    Andrology Unit, Department of Urology, Erasmus MC University Medical Centre Rotterdam, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Int J Androl 33:597-603. 2010
    ..However, the predictive value of an inhomogeneous testicular parenchyma, besides TM, for CIS is much higher...
  38. ncbi request reprint Involvement of E-cadherin and beta-catenin in germ cell tumours and in normal male fetal germ cell development
    Friedemann Honecker
    Department of Pathology, Josephine Nefkens Institute, Rotterdam, The Netherlands
    J Pathol 204:167-74. 2004
    ....
  39. ncbi request reprint Differentiation and development of human female germ cells during prenatal gonadogenesis: an immunohistochemical study
    H Stoop
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Hum Reprod 20:1466-76. 2005
    ..The present study was carried out to assess differentiation and proliferation of germ cells in a series of female gonads from 19 fetuses from the second and third trimester, and two neonates...
  40. doi request reprint A novel AMH missense mutation in a patient with persistent Müllerian duct syndrome
    Y G van der Zwan
    Department of Pediatric Endocrinology, Sophia Children s Hospital, Rotterdam, The Netherlands
    Sex Dev 6:279-83. 2012
    ..Biopsy of both gonads revealed that germ cells were present in an irregular distribution. However, the absence of OCT3/4, PLAP and c-KIT expression indicated physiological maturation...
  41. doi request reprint A pathologist's view on the testis biopsy
    J W Oosterhuis
    Department of Pathology, Josephine Nefkens Institute, Erasmus University Medical Centre, Rotterdam, The Netherlands
    Int J Androl 34:e14-9; discussion e20. 2011
    ..Preliminary results are presented on a series of biopsies from cryptorchid testes in infants and children allowing the definition of morphological and immunohistochemical criteria for delayed maturation of gonocytes and pre-CIS...
  42. ncbi request reprint Pathobiological implications of the expression of markers of testicular carcinoma in situ by fetal germ cells
    Friedemann Honecker
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Pathol 203:849-57. 2004
    ..These findings are compatible with the hypothesis that CIS/ITGCNU arises from developmentally arrested germ cells, most likely primordial germ cells/gonocytes, at an early time point during intrauterine development...
  43. ncbi request reprint Gonadoblastoma arising in undifferentiated gonadal tissue within dysgenetic gonads
    Martine Cools
    Department of Pathology, Erasmus Medical Center, University Medical Center Rotterdam, Josephine Nefkens Institute, Room 430b, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Clin Endocrinol Metab 91:2404-13. 2006
    ..The purpose of the study was to define the histological origin of gonadoblastomas, allowing the identification of high-risk patients...
  44. doi request reprint Global DNA methylation in fetal human germ cells and germ cell tumours: association with differentiation and cisplatin resistance
    Hendrik Wermann
    Department of Pathology, Erasmus MC Erasmus University Medical Center, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    J Pathol 221:433-42. 2010
    ..Among the genes revealing changes, interesting targets were identified: ie demethylation of KLF11, a putative tumour suppressor gene, and hypermethylation of CFLAR, a gene previously described in treatment resistance in GCTs...
  45. ncbi request reprint Current views on the pathogenesis of testicular germ cell tumours and perspectives for future research: highlights of the 5th Copenhagen Workshop on Carcinoma in situ and Cancer of the Testis
    J Wolter Oosterhuis
    Department of Pathology and Laboratory for Experimental Patho Oncology, Erasmus University Medical Centre and Daniel den Hoed Cancer Centre, Josephine Nefkens Institute, Rotterdam, The Netherlands
    APMIS 111:280-9. 2003
    ..The major themes that emerged at the meeting are critically discussed and perspectives for future research in this field are presented...
  46. ncbi request reprint Aneuploidy of human testicular germ cell tumors is associated with amplification of centrosomes
    Frank Mayer
    Pathology Laboratory for Experimental Patho Oncology, Josephine Nefkens Institute, Erasmus Medical Center Daniel den Hoed Cancer Center, 3000 DR Rotterdam, The Netherlands
    Oncogene 22:3859-66. 2003
    ..Therefore, aneuploidy of testicular germ cell tumors is associated with amplified centrosomes probably unrelated to STK15...
  47. ncbi request reprint Diagnostic value of OCT3/4 for pre-invasive and invasive testicular germ cell tumours
    Jeroen de Jong
    Department of Pathology, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Pathol 206:242-9. 2005
    ..The antibodies are robust and applicable with different methods of pretreatment and storage of tissue. This allows routine application of this diagnostic marker...
  48. ncbi request reprint The PTEN gene in locally progressive prostate cancer is preferentially inactivated by bi-allelic gene deletion
    P C M S Verhagen
    Department of Urology, Erasmus Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Pathol 208:699-707. 2006
    ..Taken together, these data show that bi-allelic deletion is a major mechanism of PTEN inactivation in locally progressive prostate cancer...
  49. ncbi request reprint Gene expression profiling and gene copy-number changes in malignant mesothelioma cell lines
    Claudia Zanazzi
    Department of Pathology, Erasmus Medical Center, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Genes Chromosomes Cancer 46:895-908. 2007
    ....
  50. ncbi request reprint Why human extragonadal germ cell tumours occur in the midline of the body: old concepts, new perspectives
    J Wolter Oosterhuis
    Department of Pathology, Erasmus University Medical Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Int J Androl 30:256-63; discussion 263-4. 2007
    ....
  51. ncbi request reprint Role of gain of 12p in germ cell tumour development
    Leendert H J Looijenga
    Pathology Lab for Exp Patho Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    APMIS 111:161-71; discussion 172-3. 2003
    ..So far, DAD-R is one of the most likely candidate genes involved in this process, possibly via N-glycosylation. Preliminary results on high through-put DNA- and cDNA array analyses of 12p-sequences will be presented...
  52. ncbi request reprint New insights into type II germ cell tumor pathogenesis based on studies of patients with various forms of disorders of sex development (DSD)
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Mol Cell Endocrinol 291:1-10. 2008
    ..Subsequently, the relationship between type II GCTs and DSD will be described, resulting in a number of new insights into the development of the precursor lesions of these tumors...
  53. ncbi request reprint Testicular germ-cell tumours in a broader perspective
    J Wolter Oosterhuis
    Department of Pathology, Erasmus MC, University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Nat Rev Cancer 5:210-22. 2005
    ..Recent progress begins to explain the apparent unpredictable development of germ-cell tumours and offers a basis for understanding their exquisite sensitivity to therapy...
  54. ncbi request reprint Microsatellite instability of germ cell tumors is associated with resistance to systemic treatment
    Frank Mayer
    Pathology Laboratory for Exp Patho Oncology, University Hospital Rotterdam Daniel, Josephine Nefkens Institute, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands
    Cancer Res 62:2758-60. 2002
    ..001). In 5 of all 11 unstable tumors, MSI correlated with immunohistochemical findings. This study demonstrates for the first time a positive correlation between MSI and treatment resistance in GCT...
  55. doi request reprint Recent developments in testicular germ cell tumor research
    Gert Jan M van de Geijn
    Department of Pathology, Erasmus MC, Erasmus University Medical Center, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    Birth Defects Res C Embryo Today 87:96-113. 2009
    ..In this review, a summary will be given related to several recent developments in TGCT research, especially selected because of their putative clinical impact...
  56. ncbi request reprint Maturation delay of germ cells in fetuses with trisomy 21 results in increased risk for the development of testicular germ cell tumors
    Martine Cools
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC University Medical Center, Daniel den Hoed, 3000 DR Rotterdam, The Netherlands
    Hum Pathol 37:101-11. 2006
    ....
  57. ncbi request reprint Impact of the Y-containing cell line on histological differentiation patterns in dysgenetic gonads
    Martine Cools
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, 3000 DR Rotterdam, The Netherlnads
    Clin Endocrinol (Oxf) 67:184-92. 2007
    ..The objective was to gain insight into the role of Y chromosome distribution in the histological heterogeneity of gonads of patients with gonadal dysgenesis...
  58. ncbi request reprint Germ cell lineage differentiation in non-seminomatous germ cell tumours
    Friedemann Honecker
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Rotterdam, The Netherlands
    J Pathol 208:395-400. 2006
    ..Our finding indicates a previously unknown totipotent potential of human GCTs and raises the question of whether, under certain culture conditions, primordial germ cells could be derived from human GCT cell lines...
  59. pmc A novel SRY missense mutation affecting nuclear import in a 46,XY female patient with bilateral gonadoblastoma
    Remko Hersmus
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Eur J Hum Genet 17:1642-9. 2009
    ....
  60. ncbi request reprint Noninvasive detection of testicular carcinoma in situ in semen using OCT3/4
    Niels J van Casteren
    Department of Urology, Section Andrology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Eur Urol 54:153-8. 2008
    ..We performed a study to detect CIS cells in semen using the highly specific immunohistochemical marker OCT3/4, potentially a method for noninvasive diagnosis...
  61. doi request reprint Heterogeneous distribution of ITGCNU in an adult testis: consequences for biopsy-based diagnosis
    Niels J van Casteren
    Department of Andrology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Int J Surg Pathol 16:21-4. 2008
    ....
  62. ncbi request reprint Further characterization of the first seminoma cell line TCam-2
    Jeroen de Jong
    Department of Pathology, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Genes Chromosomes Cancer 47:185-96. 2008
    ..In conclusion, TCam-2 is the first well-characterized seminoma-derived cell line, with an exceptional mutation, rarely found in TGCTs...
  63. ncbi request reprint Role of P53 and MDM2 in treatment response of human germ cell tumors
    Anne Marie F Kersemaekers
    Department of Pathology Laboratory for Experimental Patho Oncology, University Hospital Rotterdam Daniel, Josephine Nefkens Institute, Erasmus University Rotterdam, Rotterdam, The Netherlands
    J Clin Oncol 20:1551-61. 2002
    ..Testicular germ cell tumors (TGCTs) of adolescents and adults are very sensitive to systemic treatment. The exquisite chemosensitivity of these cancers has been attributed to a high level of wild-type P53...
  64. pmc Resistance to platinum-containing chemotherapy in testicular germ cell tumors is associated with downregulation of the protein kinase SRPK1
    Paul W Schenk
    Department of Medical Oncology, Erasmus MC, Rotterdam, The Netherlands
    Neoplasia 6:297-301. 2004
    ..001). In conclusion, our data suggest that SRPK1 expression might be an important prognostic indicator for the chemoresponsiveness of nonseminomatous GCTs...
  65. ncbi request reprint 12p-amplicon structure analysis in testicular germ cell tumors of adolescents and adults by array CGH
    Gaetano Zafarana
    Pathology Laboratory for Exp Patho Oncology, Erasmus MC Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Oncogene 22:7695-701. 2003
    ..These data support a mechanistic model in which at least two 12p genes, situated at the border regions of the amplicon, are positional candidates capable of actively supporting tumor progression in TGCTs...
  66. ncbi request reprint Xeroderma pigmentosum group a protein and chemotherapy resistance in human germ cell tumors
    Friedemann Honecker
    Department of Pathology, Laboratory for Experimental Patho Oncology, Josephine Nefkens Institute, Erasmus MC, University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Lab Invest 83:1489-95. 2003
    ..We therefore conclude that XPA does not play a critical role in overall treatment resistance of GCTs...
  67. ncbi request reprint Germ cell tumors in the intersex gonad: old paths, new directions, moving frontiers
    Martine Cools
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, The Netherlands
    Endocr Rev 27:468-84. 2006
    ..A new classification system for patients with DSD is proposed as a tool to refine our insight in the prevalence of germ cell tumors in specific diagnostic groups...
  68. ncbi request reprint Stem cell marker OCT3/4 in tumor biology and germ cell tumor diagnostics: history and future
    Jeroen de Jong
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed, Rotterdam, The Netherlands
    Crit Rev Oncog 12:171-203. 2006
    ..An overview of the upstream and downstream factors in the OCT3/4 pathway as well as the epigenetic regulation of the gene is summarized and the possible role in oncogenesis considered...
  69. ncbi request reprint VASA is a specific marker for both normal and malignant human germ cells
    Anne Marie Zeeman
    Department of Pathology Laboratory for Experimental Patho Oncology, University Hospital of Rotterdam Daniel, Josephine Nefkens Institute, Erasmus University Rotterdam, The Netherlands
    Lab Invest 82:159-66. 2002
    ..Furthermore, these findings suggest that VASA is likely to serve as a useful and highly specific biomarker for germ cell tumors, particularly classic and spermatocytic seminoma/dysgerminoma, including their precursor stages...
  70. ncbi request reprint Molecular determinants of treatment response in human germ cell tumors
    Frank Mayer
    Department of Pathology Laboratory for Experimental Patho Oncology, Erasmus Medical Center Daniel, Josephine Nefkens Institute, 3000 DR Rotterdam, The Netherlands
    Clin Cancer Res 9:767-73. 2003
    ..Various cellular pathways may influence the efficacy of chemotherapy. Their impact has not been investigated in a comprehensive study of tumor samples from clinically defined subgroups of GCT patients...
  71. ncbi request reprint JKT-1 is not a human seminoma cell line
    Jeroen de Jong
    Department of Pathology, Erasmus MC University Medical Center Rotterdam, Josephine Nefkens Institute, Daniel den Hoed Cancer Center
    Int J Androl 30:350-65. 2007
    ..All results indicate that the JKT-1 cell line is not representative of human seminoma. Although it can originate from an early germ cell, a non-germ cell derivation cannot be excluded...
  72. ncbi request reprint Involvement of the Fas/FasL pathway in the pathogenesis of germ cell tumours of the adult testis
    Anne Marie F Kersemaekers
    Pathology Laboratory for Exp Patho Oncology, University Hospital Rotterdam Daniel, Josephine Nefkens Institute, Erasmus University Rotterdam, Building Be, Room 430b, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    J Pathol 196:423-9. 2002
    ....
  73. ncbi request reprint Coamplification of DAD-R, SOX5, and EKI1 in human testicular seminomas, with specific overexpression of DAD-R, correlates with reduced levels of apoptosis and earlier clinical manifestation
    Gaetano Zafarana
    Pathology Laboratory for Experimental Patho Oncology, University Hospital Rotterdam Daniel, Josephine Nefkens Institute, 3000 DR Rotterdam, The Netherlands
    Cancer Res 62:1822-31. 2002
    ..In addition, our results also imply differences in expression regulation of DAD-R between seminomas and nonseminomas...
  74. ncbi request reprint Raman spectroscopic analysis identifies testicular microlithiasis as intratubular hydroxyapatite
    B W D De Jong
    Department of Pediatric Urology, Erasmus Medical Center, University Medical Center, Rotterdam, The Netherlands
    J Urol 171:92-6. 2004
    ..We analyzed the molecular composition of gonadal microlithiasis and its surrounding region using Raman spectroscopy in malignant and benign conditions...
  75. ncbi request reprint Chromosomal anomalies in oligodendroglial tumors are correlated with clinical features
    Martin J van den Bent
    Department of Neurooncology, Erasmus University Medical Center Rotterdam Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cancer 97:1276-84. 2003
    ..The authors investigated the correlation between genetic and clinical characteristics of OD in 33 patients who received chemotherapy with procarbazine, lomustine, and vincristine for recurrent disease after receiving radiotherapy...
  76. ncbi request reprint Pathobiology of testicular germ cell tumors: views and news
    Leendert H J Looijenga
    Department of Pathology, Erasmus Medical Center, University Medical Center Rotterdam, The Netherlands
    Anal Quant Cytol Histol 24:263-79. 2002
    ..In addition, the available data on the molecular basis of treatment sensitivity and resistance of GCT are reviewed...
  77. ncbi request reprint Morphological and immunohistochemical differences between gonadal maturation delay and early germ cell neoplasia in patients with undervirilization syndromes
    Martine Cools
    Department of Pathology, Erasmus MC, University Medical Center Rotterdam, Josephine Nefkens Institute, Room 430b, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Clin Endocrinol Metab 90:5295-303. 2005
    ..A developmentally delayed germ cell resembles a CIS cell and displays prolonged expression of immunohistochemical markers used for the diagnosis of CIS. This questions their applicability in young children...
  78. ncbi request reprint Successful treatment of low-grade oligodendroglial tumors with a chemotherapy regimen of procarbazine, lomustine, and vincristine
    Elize M Biemond ter Stege
    Department of Neurology Neuro Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    Cancer 103:802-9. 2005
    ..The authors retrospectively studied the outcome of the procarbazine, lomustine, and vincristine (PCV) chemotherapy regimen in a group of 16 patients with newly diagnosed OD and 5 patients with recurrent low-grade OD...
  79. ncbi request reprint New insights into the pathology and molecular biology of human germ cell tumors
    Friedemann Honecker
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Room 430b, P O Box 1738 3000 DR Rotterdam, The Netherlands
    World J Urol 22:15-24. 2004
    ..The pluripotency of GCTs, in particular the possible loss of the embryonic characteristics and acquisition of somatic differentiation, could be a crucial phenomenon in this process...
  80. ncbi request reprint The pluripotency homeobox gene NANOG is expressed in human germ cell tumors
    Adam H Hart
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia
    Cancer 104:2092-8. 2005
    ..Here we investigate the expression and gene copy number of NANOG in human germ cells and tumors of germ cell origin...
  81. ncbi request reprint Germ cell tumours in neonates and infants: a distinct subgroup?
    Imke M Veltman
    Department of Human Genetics, University Medical Center Nijmegen, Nijmegen, The Netherlands
    APMIS 111:152-60; discussion 160. 2003
    ..Recently, two cases of infantile GCT were detected with constitutional 12q13 translocations. These exceptional cases may be instrumental in the search for candidate genes related to infantile and/or adult GCT development...
  82. ncbi request reprint A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors
    P Mathijs Voorhoeve
    Division of Tumour Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cell 124:1169-81. 2006
    ..We provide evidence that these miRNAs are potential novel oncogenes participating in the development of human testicular germ cell tumors by numbing the p53 pathway, thus allowing tumorigenic growth in the presence of wild-type p53...
  83. ncbi request reprint Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas
    Dan Qiao
    Department of Cell Biology, Duke University Medical Center, PO Box 3709, DUMC, Durham, North Carolina, NC 27710, USA
    Oncogene 21:3988-99. 2002
    ..Thus, hiwi is specifically expressed in both normal and malignant spermatogenic cells in a maturation stage-dependent pattern, in which it might function in germ cell proliferation...
  84. pmc The Y deletion gr/gr and susceptibility to testicular germ cell tumor
    Katherine L Nathanson
    Department of Medicine, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Am J Hum Genet 77:1034-43. 2005
    ..0; 95% CI 1.6-5.4; P = .0004) than with nonseminoma TGCT (aOR 1.5; 95% CI 0.72-3.0; P = .29). These data indicate that the Y microdeletion gr/gr is a rare, low-penetrance allele that confers susceptibility to TGCT...
  85. pmc The Y-encoded TSPY protein: a significant marker potentially plays a role in the pathogenesis of testicular germ cell tumors
    Yunmin Li
    Department of Medicine, VA Medical Center, University of California, San Francisco, CA 94121, USA
    Hum Pathol 38:1470-81. 2007
    ..Our results suggest that TSPY, in combination with other markers, could be an important marker for diagnosis and subclassification of TGCTs and support its role in the pathogenesis of both gonadoblastoma and TGCTs...
  86. ncbi request reprint Sequence analysis of the protein kinase gene family in human testicular germ-cell tumors of adolescents and adults
    Graham Bignell
    Cancer Genome Project, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, United Kingdom
    Genes Chromosomes Cancer 45:42-6. 2006
    ..Moreover, they demonstrate that the general prevalence of point mutations in TGCT is low, in contrast to the high frequency of copy number changes...
  87. ncbi request reprint Characteristics of testicular dysgenesis syndrome and decreased expression of SRY and SOX9 in Frasier syndrome
    Valerie Schumacher
    Institute of Human Genetics, Heinrich Heine University, Duesseldorf, Germany
    Mol Reprod Dev 75:1484-94. 2008
    ..To further study the function of human Sertoli cells in the future, we have established a human cell line...
  88. doi request reprint A 40-year-old woman with a progressive periventricular white matter lesion
    Jeroen de Jong
    Brain Pathol 18:103-4, 142. 2008
  89. ncbi request reprint Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults
    Alan McIntyre
    Molecular Cytogenetics, Section of Molecular Carcinogenesis, The Institute of Cancer Research, Sutton, Surrey, United Kingdom
    Cancer Res 65:8085-9. 2005
    ....
  90. ncbi request reprint IGF2/H19 imprinting analysis of human germ cell tumors (GCTs) using the methylation-sensitive single-nucleotide primer extension method reflects the origin of GCTs in different stages of primordial germ cell development
    Sonja Sievers
    Clinic of Pediatric Oncology, Haematology and Immunology, Medical Center, Heinrich Heine University, Moorenstrasse 5, 40225 Dusseldorf, Germany
    Genes Chromosomes Cancer 44:256-64. 2005
    ..c) 2005 Wiley-Liss, Inc...
  91. ncbi request reprint A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors
    P Mathijs Voorhoeve
    Division of Tumour Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Adv Exp Med Biol 604:17-46. 2007
    ..We provide evidence that these miRNAs are potential novel oncogenes participating in the development of human testicular germ cell tumors by numbing the p53 pathway, thus allowing tumorigenic growth in the presence of wild-type p53...
  92. doi request reprint A pure triphasic testicular wilms tumor of primordial germ cell origin
    Leendert H J Looijenga
    Urology 72:232-3; author reply 233. 2008
  93. ncbi request reprint Are all testicular seminomas of animals in fact spermatocytic seminomas?
    Leendert H J Looijenga
    Vet Pathol 44:126. 2007
  94. ncbi request reprint CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells
    Daniella Herszfeld
    Monash Institute of Medical Research, Monash University, and the Australian Stem Cell Centre, Bldg 75 STRIP, Wellington Road, Clayton, Victoria 3800 Australia
    Nat Biotechnol 24:351-7. 2006
    ..Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis...
  95. ncbi request reprint Lung cancer: intragenic ERBB2 kinase mutations in tumours
    Philip Stephens
    Cancer Genome Project, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK
    Nature 431:525-6. 2004
    ..ERBB2 inhibitors, which have so far proved to be ineffective in treating lung cancer, should now be clinically re-evaluated in the specific subset of patients with lung cancer whose tumours carry ERBB2 mutations...
  96. ncbi request reprint Fusion of the SUMO/Sentrin-specific protease 1 gene SENP1 and the embryonic polarity-related mesoderm development gene MESDC2 in a patient with an infantile teratoma and a constitutional t(12;15)(q13;q25)
    Imke M Veltman
    Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Hum Mol Genet 14:1955-63. 2005
    ..Together, the constitutional t(12;15)(q13;q25) translocation revealed two novel candidate genes for neonatal/infantile GCT development: MESDC2 and SENP1...
  97. ncbi request reprint Somatic mutations of the protein kinase gene family in human lung cancer
    Helen Davies
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, United Kingdom
    Cancer Res 65:7591-5. 2005
    ..The results suggest that several mutated protein kinases may be contributing to lung cancer development, but that mutations in each one are infrequent...