Research Topics
| C H J LamersSummaryAffiliation: Erasmus MC Country: The Netherlands Publications
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Detail Information
Publications
Protocol for gene transduction and expansion of human T lymphocytes for clinical immunogene therapy of cancerCor H J Lamers
Department of Medical Oncology, Subdivision of Clinical and Tumor Immunology, Erasmus Medical Center Daniel, Rotterdam, The Netherlands
Cancer Gene Ther 9:613-23. 2002..This clinical protocol routinely yields 30-65% scFvG250 ch-Rec(POS) T lymphocytes in both healthy donors and RCC patients...
Treatment of metastatic renal cell carcinoma with CAIX CAR-engineered T cells: clinical evaluation and management of on-target toxicityCor Hj Lamers
Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Mol Ther 21:904-12. 2013..We provide in-patient proof that the observed "on-target" toxicity is antigen-directed and can be prevented by blocking antigenic sites in off-tumor organs and allowing higher T cell doses...
Long-term stability of T-cell activation and transduction components critical to the processing of clinical batches of gene-engineered T cellsCor H J Lamers
Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Cytotherapy 15:620-6. 2013....
Immune responses to transgene and retroviral vector in patients treated with ex vivo-engineered T cellsCor H J Lamers
Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Blood 117:72-82. 2011....
Gibbon ape leukemia virus poorly replicates in primary human T lymphocytes: implications for safety testing of primary human T lymphocytes transduced with GALV-pseudotyped vectorsCor H J Lamers
Unit of Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, AE Rotterdam, The Netherlands
J Immunother 32:272-9. 2009....
Retronectin-assisted retroviral transduction of primary human T lymphocytes under good manufacturing practice conditions: tissue culture bag critically determines cell yieldC H J Lamers
Laboratory of Clinical and Tumor Immunology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Cytotherapy 10:406-16. 2008..Critical to our successful clinical-scale transductions of patient T cells was the use of Retronectin in combination with Lifecell X-foldtrade mark cell culture bags...
Retroviral vectors for clinical immunogene therapy are stable for up to 9 yearsC H J Lamers
Department of Medical Oncology, Laboratory of Clinical and Tumor Immunology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Cancer Gene Ther 15:268-74. 2008..These data provide evidence that in terms of 'life expectancy' the production and storage of clinical batches of RTVsup for gene therapy warrants the corresponding professional and financial risks...
Gene-modified T cells for adoptive immunotherapy of renal cell cancer maintain transgene-specific immune functions in vivoCor H J Lamers
Laboratory for Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC, Daniel den Hoed Cancer Center, PO Box 5201, 3008 AE, Rotterdam, The Netherlands
Cancer Immunol Immunother 56:1875-83. 2007..Here, we investigate whether or not the in vivo activity of the infused scFv(G250)(+) T cells is reflected by changes of selected immune parameters measured in peripheral blood...
Process validation and clinical evaluation of a protocol to generate gene-modified T lymphocytes for imunogene therapy for metastatic renal cell carcinoma: GMP-controlled transduction and expansion of patient's T lymphocytes using a carboxy anhydrase IX-sC H J Lamers
Unit Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus University Medical Center Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Cytotherapy 8:542-53. 2006..We describe the validation of our clinical protocol for gene transduction and expansion of human T lymphocytes...
Adoptive immuno-gene therapy of cancer with single chain antibody [scFv(Ig)] gene modified T lymphocytesC H J Lamers
Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
J Biol Regul Homeost Agents 18:134-40. 2004..Currently, we evaluate this concept in a clinical protocol to treat patients with metastatic renal cell cancer (RCC) using autologous RCC-specific gene-modified T lymphocytes...
Parallel detection of transduced T lymphocytes after immunogene therapy of renal cell cancer by flow cytometry and real-time polymerase chain reaction: implications for loss of transgene expressionC H J Lamers
Laboratory of Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, 3008 AE Rotterdam, The Netherlands
Hum Gene Ther 16:1452-62. 2005..These results suggest loss of scFv(G250) membrane expression on adoptive transfer, which would have important implications for the antitumor efficacy of this form of immunogene therapy...
Phoenix-ampho outperforms PG13 as retroviral packaging cells to transduce human T cells with tumor-specific receptors: implications for clinical immunogene therapy of cancerC H J Lamers
Unit of Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus MC Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Cancer Gene Ther 13:503-9. 2006..The improved functional transduction efficiency together with a limited increase in the number of integrations per recipient cell, made us select Phoenix clone 58 for our clinical immunogene therapy study...
T-lymphocyte reconstitution following rigorously T-cell-depleted versus unmodified autologous stem cell transplantsP A W te Boekhorst
Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands
Bone Marrow Transplant 37:763-72. 2006..This susceptibility to apoptosis would interfere with a swift and sustained CD4(+) T-cell regeneration post SCT...
Bone-marrow transplantation fails to halt intrathecal lymphocyte activation in multiple sclerosisT Mondria
Department of Neurology, Erasmus University Medical School, Erasmus MC, 3000 CA Rotterdam, The Netherlands
J Neurol Neurosurg Psychiatry 79:1013-5. 2008..Given the presumed key role for autoreactive lymphocytes in multiple sclerosis (MS), treatment strategies have been developed to ablate lymphocyte activity. Intrathecal lymphocyte activation can be measured by CSF-soluble(s)CD27...
Repeated administrations of interleukin (IL)-12 are associated with persistently elevated plasma levels of IL-10 and declining IFN-gamma, tumor necrosis factor-alpha, IL-6, and IL-8 responsesJohanna E A Portielje
Department of Medical Oncology, Erasmus MC - Daniel den Hoed, 3075 EA Rotterdam, The Netherlands
Clin Cancer Res 9:76-83. 2003..The steady increment of IL-10 plasma levels may mediate the observed down-regulation of clinical and immunological effects...
mRNA levels of CD31, CD144, CD146 and von Willebrand factor do not serve as surrogate markers for circulating endothelial cellsMichiel H Strijbos
Laboratory of Translational Tumorimmunolgy, Department of Medical Oncology, Erasmus University Medical Center, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
Thromb Haemost 104:318-26. 2010..We conclude that mRNA levels of CD31, CD144, CD146 and vWf in whole blood as detected by real time RT-PCR cannot be used as biomarkers for end-stage endothelial cells such as CEC...
Immunologic analysis of a phase I/II study of vaccination with MAGE-3 protein combined with the AS02B adjuvant in patients with MAGE-3-positive tumorsValérie Vantomme
GlaxoSmithKline Biologicals, Rixensart, Belgium
J Immunother 27:124-35. 2004..It is concluded that vaccination of advanced cancer patients with MAGE-3 self-antigen in AS02B adjuvant is able to elicit MAGE-3-specific antibody and a T-cell response...
Treatment of metastatic renal cell carcinoma with autologous T-lymphocytes genetically retargeted against carbonic anhydrase IX: first clinical experienceCor H J Lamers
J Clin Oncol 24:e20-2. 2006
