Research Topics
Genomes and Genes | Johannes G KustersSummaryAffiliation: Erasmus MC Country: The Netherlands Publications
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Publications
Pathogenesis of Helicobacter pylori infectionJohannes G Kusters
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Clin Microbiol Rev 19:449-90. 2006..This review focuses on the microbiological, clinical, immunological, and biochemical aspects of the pathogenesis of H. pylori...
Aneuploidy and overexpression of Ki67 and p53 as markers for neoplastic progression in Barrett's esophagus: a case-control studyMarjolein Sikkema
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
Am J Gastroenterol 104:2673-80. 2009..The aim of this study was to determine the predictive value of p53, Ki67, and aneuploidy as markers of neoplastic progression in BE...- Iron-responsive regulation of the Helicobacter pylori iron-cofactored superoxide dismutase SodB is mediated by FurFlorian D Ernst
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dijkzigt L 4, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Bacteriol 187:3687-92. 2005..In conclusion, H. pylori Fur is a versatile regulator involved in many pathways essential for gastric colonization, including superoxide stress defense... - Iron-responsive repression of urease expression in Helicobacter hepaticus is mediated by the transcriptional regulator FurClara Belzer
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, 3015 CE Rotterdam, The Netherlands
Infect Immun 75:745-52. 2007..This represents a novel type of urease regulation in ureolytic bacteria and extends the already diverse regulatory repertoire of the Fur protein... 
Inhibition of viral replication reduces regulatory T cells and enhances the antiviral immune response in chronic hepatitis BJeroen N Stoop
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, s Gravendijkwal 230, Room Ca 326, 3015 CE Rotterdam, The Netherlands
Virology 361:141-8. 2007..The production of IL10 did not decrease during therapy. In conclusion, adefovir induced viral load reduction results in a decline of circulating Treg together with a partial recovery of the immune response...- Acid-responsive gene induction of ammonia-producing enzymes in Helicobacter pylori is mediated via a metal-responsive repressor cascadeArnoud H M van Vliet
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
Infect Immun 72:766-73. 2004..5 in wild-type H. pylori. We hypothesize that H. pylori uses a repressor cascade to respond to low pH, with NikR initiating the response directly via the urease operon and indirectly via the members of the Fur regulon... - The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pyloriFlorian D Ernst
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Infect Immun 73:7252-8. 2005..In conclusion, the NikR protein is able to function both as a repressor and as an activator of gene transcription, depending on the position of the binding site... - The functional status of the Helicobacter pylori sabB adhesin gene as a putative marker for disease outcomeRamon de Jonge
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands
Helicobacter 9:158-64. 2004..Assuming that SabB is involved in bacterial adhesion, this association suggests that adherent H. pylori are more prone to elimination by the host immune system... - Transcriptional profiling of Helicobacter pylori Fur- and iron-regulated gene expressionFlorian D Ernst
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Dijkzigt L 455, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Microbiology 151:533-46. 2005..pylori Fur is a versatile regulator involved in many pathways essential for gastric colonization. These findings further delineate the central role of Fur in regulating the unique capacity of H. pylori to colonize the human stomach... - Expression, localization and polymorphisms of the nuclear receptor PXR in Barrett's esophagus and esophageal adenocarcinomaAnouk van de Winkel
Department of Gastroenterology and Hepatology, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
BMC Gastroenterol 11:108. 2011..This study aimed to investigate the expression and genetic variation of PXR in reflux esophagitis (RE), Barrett's esophagus (BE) and esophageal adenocarcinoma... - NikR mediates nickel-responsive transcriptional repression of the Helicobacter pylori outer membrane proteins FecA3 (HP1400) and FrpB4 (HP1512)Florian D Ernst
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Room L 455, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
Infect Immun 74:6821-8. 2006..pylori to the gastric lifestyle... - Differential regulation of amidase- and formamidase-mediated ammonia production by the Helicobacter pylori fur repressorArnoud H M van Vliet
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
J Biol Chem 278:9052-7. 2003..These roles of Fur in regulation of the H. pylori amidases suggest that the H. pylori Fur regulator may have acquired extra functions to compensate for the absence of other regulatory systems... - NcoI TNF-β gene polymorphism and TNF expression are associated with an increased risk of developing Barrett's esophagus and esophageal adenocarcinomaVivianda Menke
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
Scand J Gastroenterol 47:378-86. 2012..The aim of the study was to determine the impact of TNF gene variation on the RE-BE-EAC cascade... - UreA2B2: a second urease system in the gastric pathogen Helicobacter felisRaymond G J Pot
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
FEMS Immunol Med Microbiol 50:273-9. 2007..Although the exact function of the UreA2B2 system is still unknown, it is conceivable that UreA2B2 may contribute to pathogenesis of H. felis infection through a yet unknown mechanism... - Favorable effect of adefovir on the number and functionality of myeloid dendritic cells of patients with chronic HBVRenate G van der Molen
Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
Hepatology 44:907-14. 2006..Our findings provide clues for the reasons why current antiviral therapy does not lead to consistently sustained viral eradication... - The Helicobacter hepaticus hefA gene is involved in resistance to amoxicillinClara Belzer
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
Helicobacter 14:72-9. 2009..In this study we have determined the susceptibility of gastric and enterohepatic Helicobacter species to amoxicillin, and have investigated the mechanism of amoxicillin resistance in Helicobacter hepaticus... - The Helicobacter pylori plasticity region locus jhp0947-jhp0949 is associated with duodenal ulcer disease and interleukin-12 production in monocyte cellsRamon de Jonge
Department of Gastroenterology and Hepatology, Room L-459, Erasmus MC-University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
FEMS Immunol Med Microbiol 41:161-7. 2004..027). Therefore, the jhp0947-jhp0949 locus may be a novel putative H. pylori marker for disease outcome independent of the cag PAI... - Inverse nickel-responsive regulation of two urease enzymes in the gastric pathogen Helicobacter mustelaeJeroen Stoof
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
Environ Microbiol 10:2586-97. 2008..Pathogens often express colonization factors which are adapted to their host. The UreA2B2 urease could represent an example of pathogen adaptation to the specifics of the diet of their carnivorous host, rather than to the host itself... - Bile acid-stimulated expression of the farnesoid X receptor enhances the immune response in Barrett esophagusAstrid Capello
Department of Gastroenterology and Hepatology, Erasmus Universiry Medical Center, Rotterdam, The Netherlands
Am J Gastroenterol 103:1510-6. 2008..FXR activation is also involved in the induction of the innate immune response. This suggests that FXR is involved in the pathogenesis and the inflammation seen in BE... - Role of the rdxA and frxA genes in oxygen-dependent metronidazole resistance of Helicobacter pyloriMonique M Gerrits
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
J Med Microbiol 53:1123-8. 2004..As there were no significant differences in cell viability between the clinical isolates, it is likely that neither the rdxA nor the frxA gene participates in the reversibility of metronidazole resistance... - Multiple mutations in or adjacent to the conserved penicillin-binding protein motifs of the penicillin-binding protein 1A confer amoxicillin resistance to Helicobacter pyloriMonique M Gerrits
Department of Gastroenterology and Hepatology, Erasmus MC - University Medical Center, Rotterdam, The Netherlands
Helicobacter 11:181-7. 2006..pylori. The findings of this study currently preclude the rapid detection of amoxicillin resistance in H. pylori by molecular tests... - Tumor necrosis factor alpha inhibits the suppressive effect of regulatory T cells on the hepatitis B virus-specific immune responseJeroen N Stoop
Department of Gastroenterology and Hepatology, Erasmus MC Rotterdam, The Netherlands
Hepatology 46:699-705. 2007..Because chronic HBV patients are known to produce less TNF-alpha, these data implicate an important role for TNF-alpha in the impaired antiviral response in chronic HBV... - Induction of regulatory T-cells and interleukin-10-producing cells in non-responders to pegylated interferon-alpha therapy for chronic hepatitis BDave Sprengers
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Centre, Rotterdam, The Netherlands
Antivir Ther 12:1087-96. 2007..We aimed to investigate whether in non-responders to IFN-alpha therapy Treg contribute to treatment failure by downregulating the HBV-specific T-cell responses... - Aneuploidy and high expression of p53 and Ki67 is associated with neoplastic progression in Barrett esophagusMarjon Kerkhof
Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
Cancer Biomark 4:1-10. 2008..Accumulation of p53 was seen several years before development of HGD/EAC, and may therefore be an early marker in BE at a stage when dysplasia is not yet detected. A prospective follow-up study is needed to confirm these findings... - Transcriptional phase variation of a type III restriction-modification system in Helicobacter pyloriNicolette de Vries
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands
J Bacteriol 184:6615-23. 2002..Moreover, the C tract was required for the occurrence of transcriptional phase variation. Our finding that translation and transcription are linked during phase variation through slipped-strand mispairing is new for H. pylori... - Differential regulation of urease activity in Helicobacter hepaticus and Helicobacter pyloriClara Belzer
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Microbiology 151:3989-95. 2005..In conclusion, both the role of the urease enzyme and the regulation of urease activity differ between the enterohepatic pathogen H. hepaticus and the gastric pathogen H. pylori... - Role of the Helicobacter pylori outer-membrane proteins AlpA and AlpB in colonization of the guinea pig stomachRamon de Jonge
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands
J Med Microbiol 53:375-9. 2004..05). In conclusion, absence of AlpA or AlpB is a serious disadvantage for colonization of the stomach by H. pylori... - COX-2 CA-haplotype is a risk factor for the development of esophageal adenocarcinomaLeon M G Moons
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
Am J Gastroenterol 102:2373-9. 2007..Neoplastic progression of BE towards EAC is associated with increased expression of COX-2. Increased COX-2 expression and enzyme activity is linked to the COX-2 CA haplotype, which consists of two gene polymorphisms in the COX-2 promoter... - The role of the Ferric Uptake Regulator (Fur) in regulation of Helicobacter pylori iron uptakeArnoud H M van Vliet
Department of Gastroenterology and Hepatology, Erasmus MC, Rotterdam, The Netherlands
Helicobacter 7:237-44. 2002..Bacterial iron-responsive regulation is often mediated by Ferric Uptake Regulator (Fur) homologs, and in this study we have determined the role of H. pylori Fur in regulation of H. pylori iron uptake... - High-grade dysplasia in Barrett's esophagus is associated with increased expression of calgranulin A and BDorine A Bax
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
Scand J Gastroenterol 42:902-10. 2007..The aim of this study was to increase the understanding of the development of dysplastic lesions in BE through the identification of genes that are differentially transcribed in these tissue types... - Biomarker-based prediction of inflammatory bowel disease-related colorectal cancer: a case-control studyMonique M Gerrits
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
Cell Oncol (Dordr) 34:107-17. 2011..Screening accuracy may increase by using a biomarker-based surveillance strategy... - Barrett's oesophagus is characterized by a predominantly humoral inflammatory responseLeon M G Moons
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Centre, Rotterdam, The Netherlands
J Pathol 207:269-76. 2005..It may be that this shift, which is similar to that found in other chronic inflammatory conditions, contributes to an increased cancer risk in BO... - Myo9B is associated with an increased risk of Barrett's esophagus and esophageal adenocarcinomaVivianda Menke
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
Scand J Gastroenterol 47:1422-8. 2012..Polymorphisms in the 3' region of myosin IXB (Myo9B) are associated with chronic inflammatory gastrointestinal disorders like celiac disease and ulcerative colitis, assuming that variation in Myo9B influences the intestinal permeability... - Biomarkers for risk stratification of neoplastic progression in Barrett esophagusMarjon Kerkhof
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
Cell Oncol 29:507-17. 2007..e. proliferation/cell cycle proteins, tumor suppressor genes, adhesion molecules, DNA ploidy status and inflammation associated markers, that can be used for risk stratification in BE, and discuss their respective clinical application... - The stress-induced hsp12 gene shows genetic variation among Helicobacter pylori strainsNicolette de Vries
Department of Gastroenterology and Hepatology, Erasmus MC - University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
FEMS Immunol Med Microbiol 38:45-51. 2003..The stress-regulated, strain-specific hsp12 genes may be involved in adaptation of individual H. pylori strains to their specific hosts, and contribute to long-term colonization of the gastric niche... - Effects of 16S rRNA gene mutations on tetracycline resistance in Helicobacter pyloriMonique M Gerrits
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands
Antimicrob Agents Chemother 47:2984-6. 2003..pylori isolates... - Characterization of human liver dendritic cells in liver grafts and perfusatesBrenda M Bosma
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Rotterdam, The Netherlands
Liver Transpl 12:384-93. 2006..These viable MDC have the capacity to stimulate allogeneic T-cells, and thus may represent a major player in the induction of acute rejection... - NikR-mediated regulation of Helicobacter pylori acid adaptationArnoud H M van Vliet
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
Trends Microbiol 12:489-94. 2004..Despite the paucity of regulatory proteins, this enables H. pylori to optimally adapt to conditions in the stomach, making it one of the most successful human pathogens... - Analysis of intrahepatic HBV-specific cytotoxic T-cells during and after acute HBV infection in humansDave Sprengers
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Hepatol 45:182-9. 2006..Their presence in the liver may suggest a role in the resolution of the infection. Intrahepatic HBV-specific CD8 + T cells remain detectable at high frequencies after HBsAg-seroconversion... - Direct random insertion mutagenesis of Helicobacter pyloriRamon de Jonge
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Microbiol Methods 52:93-100. 2003..The isolation of two independent H. pylori mutants defective in production of urease from this library underlines the potential of this mutagenesis strategy... - Molecular alterations during development of esophageal adenocarcinomaDorine A Bax
Department of Gastroenterology and Hepatology, Erasmus MC - University Medical Center Rotterdam, Rotterdam, The Netherlands
J Surg Oncol 92:89-98; discussion 99. 2005..These novel developments will open new perspectives for diagnosis, treatment, and prevention of esophageal adenocarcinoma... - The intrahepatic immune response during chronic hepatitis B infection can be monitored by the fine-needle aspiration biopsy techniqueThjon J Tang
Department of Gastroenterology and Hepatology, Room L-455, Erasmus MC, University Medical Center Rotterdam, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands
FEMS Immunol Med Microbiol 39:69-72. 2003..In conclusion, the FNAB technique is suited to investigate the intrahepatic immune response during chronic hepatitis B infection on a frequent basis... - Intrahepatic CD8 T-lymphocytes and HBV core expression in relation to response to antiviral therapy for chronic hepatitis B patientsThjon J Tang
Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center Rotterdam, The Netherlands
J Med Virol 72:215-22. 2004..Response to therapy led to a significant reduction of intrahepatic CD8 T-lymphocytes. Co-localisation of CD8 T-lymphocytes and HBcAg-positive hepatocytes suggests antiviral activity predominantly at the site of maximum HBV replication... - 16S rRNA mutation-mediated tetracycline resistance in Helicobacter pyloriMonique M Gerrits
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, The Netherlands
Antimicrob Agents Chemother 46:2996-3000. 2002..This triple-base-pair substitution, present in both copies of the 16S rRNA gene of all our Tet(r) H. pylori transformants, resulted in an increased MIC of tetracycline that was identical to that for the Tet(r) strain 181... - No association between tumor necrosis factor-alpha production and gene polymorphisms among inbred rat strainsMichiel C Warlé
Department of Surgery, Erasmus MC Rotterdam, P O Box 2040, 3000 CA, Rotterdam, The Netherlands
Transpl Immunol 14:77-82. 2005..Without an established relationship between polymorphisms and expression of the TNF-alpha gene, it is preliminary to address a possible association of Tnf gene polymorphisms with rat liver allograft survival... - Detection of identical Helicobacter DNA in the stomach and in the non-cirrhotic liver of patients with hepatocellular carcinomaCornelis Verhoef
Departments of Surgical Oncology Erasmus Medical Centre, University Hospital Rotterdam, Rotterdam, The Netherlands
Eur J Gastroenterol Hepatol 15:1171-4. 2003..The possible relationship between the presence of Helicobacter species in the non-cirrhotic liver of patients with hepatocellular carcinoma (HCC) and the detection of Helicobacter species in their stomach was explored... - Functional impairment of myeloid and plasmacytoid dendritic cells of patients with chronic hepatitis BRenate G van der Molen
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
Hepatology 40:738-46. 2004..In conclusion, mDC and pDC are functionally impaired in patients with chronic hepatitis B. This might be an important way by which hepatitis B virus evades an adequate immune response, leading to viral persistence and disease chronicity... - Serum- and animal tissue-free medium for transport and growth of Helicobacter pyloriCindy M Dierikx
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
FEMS Immunol Med Microbiol 50:239-43. 2007..pylori. The formulation of SATFM may allow its use in international transport of H. pylori, and may also allow certified use in immunization studies requiring growth of H. pylori and other bacterial pathogens... - Immunomagnetic selection of functional dendritic cells from human lymph nodesPatrick P C Boor
Department of Gastroenterology and Hepatology, Room L-455, Erasmus Medical Center, Rotterdam, The Netherlands
Immunol Lett 99:162-8. 2005..Moreover, these MDC are functionally intact. Selection with anti-BDCA-4 mAb does enrich PDC from human LN, but the resulting preparations are contaminated with T- and NK-cells... - Intrahepatic CD8+ T-lymphocyte response is important for therapy-induced viral clearance in chronic hepatitis B infectionThjon J Tang
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Room L-455, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
J Hepatol 43:45-52. 2005.... - Regulatory T cells contribute to the impaired immune response in patients with chronic hepatitis B virus infectionJeroen N Stoop
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
Hepatology 41:771-8. 2005..T(reg) have an immunosuppressive effect on HBV-specific T helper cells. The presence of HBV-specific T(reg) could contribute to an inadequate immune response against the virus, leading to chronic infection... - NikR mediates nickel-responsive transcriptional induction of urease expression in Helicobacter pyloriArnoud H M van Vliet
Department of Gastroenterology and Hepatology, Erasmus Medical Center, Rotterdam, The Netherlands
Infect Immun 70:2846-52. 2002..In conclusion, the H. pylori NikR protein and a 19-bp operator sequence in the ureA promoter are both essential for nickel-responsive induction of urease expression in H. pylori... - Helicobacter species are not detectable by 16S rDNA PCR in bile from Dutch patients with common bile duct stonesRobert Roosendaal
Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands
Digestion 66:89-91. 2002..To explore the possible relation between Helicobacter spp. and gallbladder disorders, we have investigated their presence in bile of patients with biliary obstruction and dilatation of the bile ducts... - Detection of Helicobacter pylori in bile of catsSacha Y Boomkens
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 8, P O Box 80154, 3508 TD Utrecht, The Netherlands
FEMS Immunol Med Microbiol 42:307-11. 2004..44). Sequence analysis of the amplicons revealed a 100% identity with the Helicobacter pylori specific DNA fragments. Our data suggest an etiological role of H. pylori in feline LC and that cats are a potential zoonotic reservoir... - Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLPMarcelo L Ribeiro
Clinical Pharmacology and Gastroenterology Unit, , , SP, Brazil
FEMS Immunol Med Microbiol 40:57-61. 2004..This PCR-RFLP-based approach distinguishes the high-level Tet(R) isolates from low-level Tet(R) and Tet(S) H. pylori strains and thus allows the direct detection of Tet(R) H. pylori isolates... - Strain-specific in vitro cytokine production profiles do not predict rat liver allograft survivalMichiel C Warlé
Department of Surgery, Erasmus MC Rotterdam, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
Transpl Int 17:779-86. 2005.... - Induced Helicobacter pylori vacuolating cytotoxin VacA expression after initial colonisation of human gastric epithelial cellsKarin van Amsterdam
Academic Medical Center, Department of Medical Microbiology, P O Box 22660, 1100 DD Amsterdam, The Netherlands
FEMS Immunol Med Microbiol 39:251-6. 2003..These results show the applicability of this technique to study H. pylori gene regulation in its natural environment... - Colonization with cagA-positive Helicobacter pylori strains in intestinal metaplasia of the esophagus and the esophagogastric junctionPernilla Ackermark
Department of Gastroenterology, St. Antonius Hospital, Nieuwegein, The Netherlands
Am J Gastroenterol 98:1719-24. 2003..pylori or cagA-positive strains. IM at the junction and Barrett's esophagus seem to have different etiologies... - Essential role of ferritin Pfr in Helicobacter pylori iron metabolism and gastric colonizationBarbara Waidner
Institute of Medical Microbiology and Hygiene, Department of Medical Microbiology and Hygiene, University Hospital of Freiburg, Germany
Infect Immun 70:3923-9. 2002..In summary, the pfr phenotypes observed give new insights into prokaryotic ferritin functions and indicate that iron storage and homeostasis are of extraordinary importance for H. pylori to survive in its hostile natural environment... - The mouse colonizing Helicobacter pylori strain SS1 may lack a functional cag pathogenicity islandJean E Crabtree
Helicobacter 7:139-40; author reply 140-1. 2002 - Natural transformation in Helicobacter pylori: DNA transport in an unexpected wayLeonard C Smeets
Dept of Medical Microbiology and Infection Control, VU University Medical Center, PO Box 7057, 1007 MB, Amsterdam, The Netherlands
Trends Microbiol 10:159-62; discussion 162. 2002..Instead, in H. pylori it has been suggested that the five proteins that form the transmembrane channel of the transformation system are closely related to subunits of type IV secretion systems... - Novel plasmids for gene expression analysis and for genetic manipulation in the gastric pathogen Helicobacter pyloriStefan Bereswill
Department of Microbiology and Hygiene, National Reference Center for Helicobacter pylori, Institute of Medical Microbiology and Hygiene, University Hospital of Freiburg, Hermann Herder Strasse 11, D 79104 Freiburg, Germany
FEMS Immunol Med Microbiol 44:157-62. 2005..pylori, and may also be of use for other bacteria, as the resistance cassettes and the lacZ gene are active in the related Campylobacter species... - Restriction of DNA encoding selectable markers decreases the transformation efficiency of Helicobacter pyloriRebecca J Gorrell
Department of Microbiology and Immunology, University of Melbourne, Parkville VIC 3010, Australia Microbiological Research Unit, Murdoch Childrens Research Institute, Parkville VIC 3052, Australia
FEMS Immunol Med Microbiol 44:213-9. 2005..Digestion of 32P-labelled cassettes by H. pylori lysates mirrored the transformation results and indicated that in some strains these cassettes are the targets of enzymatic restriction... - The novel Helicobacter pylori CznABC metal efflux pump is required for cadmium, zinc, and nickel resistance, urease modulation, and gastric colonizationFrank Nils Stähler
Department of Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann Herder Str 11, D 79104 Freiburg, Germany
Infect Immun 74:3845-52. 2006..This demonstrates that the metal export functions of H. pylori cznABC are essential for gastric colonization and underlines the extraordinary importance of metal ion homeostasis for the survival of H. pylori in the gastric environment... - Low circulating regulatory T-cell levels after acute rejection in liver transplantationAhmet Demirkiran
Department of Surgery, Erasmus MC-University Medical Center, Rotterdam, The Netherlands
Liver Transpl 12:277-84. 2006..Despite these quantitative differences between rejectors and nonrejectors, the suppressive quality of CD4+CD25+ Tregs is identical in both groups... - Of microbe and man: determinants of Helicobacter pylori-related diseasesKarin van Amsterdam
Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands
FEMS Microbiol Rev 30:131-56. 2006..In this review, we discuss host, environmental and bacterial factors that are important in disease progression. Moreover, H. pylori adaptive mechanisms, which allow its life-long survival and growth in the gastric mucosa are considered... - IL-1 RN polymorphism is not associated with Barrett's esophagus and esophageal adenocarcinomaLeon M G Moons
Am J Gastroenterol 100:2818. 2005 - Helicobacter pylori HopH (OipA) and bacterial pathogenicity: genetic and functional genomic analysis of hopH gene polymorphismsAnar Dossumbekova
Second Department of Internal Medicine and Gastroenterology, Technical University of Munich, Munich, Germany
J Infect Dis 194:1346-55. 2006..The strong linkage with cagA indicates that HopH may contribute to the fitness of cagA-positive strains in vivo... - Real-time PCR screening for 16S rRNA mutations associated with resistance to tetracycline in Helicobacter pyloriErik Glocker
, Abteilung Medizinische Mikrobiologie und Hygiene, , , Hermann-Herder-Strasse 11, D-79104 Freiburg, Germany
Antimicrob Agents Chemother 49:3166-70. 2005..pylori-infected gastric biopsy samples. We therefore conclude that this real-time PCR is an excellent method for determination of H. pylori tetracycline resistance even when live bacteria are no longer available... - The presence of the cag pathogenicity island is associated with increased superoxide anion radical scavenging activity by Helicobacter pyloriJacob J Briedé
Department of Health Risk Analysis and Toxicology, Faculty of Health Sciences, Maastricht University, Maastricht, The Netherlands
FEMS Immunol Med Microbiol 44:227-32. 2005..We hypothesize that the increased scavenging activity of cag-positive H. pylori strains is an adaptation to the increased inflammatory response associated with the cag-positive genotype of H. pylori... - Random mutagenesis to identify novel Helicobacter mustelae virulence factorsTadhg O Cróinín
Children s Research Centre, Our Lady s Hospital for Sick Children, Crumlin, Dublin, Ireland
FEMS Immunol Med Microbiol 50:257-63. 2007..The development of a mutant library for a natural animal model of Helicobacter infection provides the opportunity to study in vivo the role of candidate Helicobacter virulence genes... - Differentially expressed genes in response to amoxicillin in Helicobacter pylori analyzed by RNA arbitrarily primed PCRAnita P O Godoy
Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Braganca Paulista, SP, Brazil
FEMS Immunol Med Microbiol 50:226-30. 2007..Further analysis of these cDNAs will allow a better comprehension of both the molecular mechanism(s) of amoxicillin resistance and the adaptative mechanism(s) used by H. pylori in the presence of this antibiotic... - The role of Helicobacter spp. in the pathogenesis of primary biliary cirrhosis and primary sclerosing cholangitisSacha Y Boomkens
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
FEMS Immunol Med Microbiol 44:221-5. 2005..Sequence analysis confirmed Helicobacter spp. DNA. Because Helicobacter spp. DNA can be found in approximately one-third of all samples tested, it is unlikely that PSC and PBC are caused by Helicobacter infection... - The Helicobacter pylori homologue of the ferric uptake regulator is involved in acid resistanceJetta J E Bijlsma
Department of Medical Microbiology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands
Infect Immun 70:606-11. 2002..From this we conclude that Fur is involved in the growth at acidic pH of H. pylori; as such, it is the first regulatory protein implicated in the acid resistance of this important human pathogen...