Johannes G Kusters

Summary

Affiliation: Erasmus MC
Country: The Netherlands

Publications

  1. pmc Pathogenesis of Helicobacter pylori infection
    Johannes G Kusters
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Clin Microbiol Rev 19:449-90. 2006
  2. doi request reprint Aneuploidy and overexpression of Ki67 and p53 as markers for neoplastic progression in Barrett's esophagus: a case-control study
    Marjolein Sikkema
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Am J Gastroenterol 104:2673-80. 2009
  3. pmc Iron-responsive regulation of the Helicobacter pylori iron-cofactored superoxide dismutase SodB is mediated by Fur
    Florian D Ernst
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dijkzigt L 4, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Bacteriol 187:3687-92. 2005
  4. pmc Iron-responsive repression of urease expression in Helicobacter hepaticus is mediated by the transcriptional regulator Fur
    Clara Belzer
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, 3015 CE Rotterdam, The Netherlands
    Infect Immun 75:745-52. 2007
  5. ncbi request reprint Inhibition of viral replication reduces regulatory T cells and enhances the antiviral immune response in chronic hepatitis B
    Jeroen N Stoop
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, s Gravendijkwal 230, Room Ca 326, 3015 CE Rotterdam, The Netherlands
    Virology 361:141-8. 2007
  6. pmc The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori
    Florian D Ernst
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Infect Immun 73:7252-8. 2005
  7. pmc Acid-responsive gene induction of ammonia-producing enzymes in Helicobacter pylori is mediated via a metal-responsive repressor cascade
    Arnoud H M van Vliet
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Infect Immun 72:766-73. 2004
  8. ncbi request reprint The functional status of the Helicobacter pylori sabB adhesin gene as a putative marker for disease outcome
    Ramon de Jonge
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Helicobacter 9:158-64. 2004
  9. ncbi request reprint Transcriptional profiling of Helicobacter pylori Fur- and iron-regulated gene expression
    Florian D Ernst
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Dijkzigt L 455, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbiology 151:533-46. 2005
  10. doi request reprint Myo9B is associated with an increased risk of Barrett's esophagus and esophageal adenocarcinoma
    Vivianda Menke
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Scand J Gastroenterol 47:1422-8. 2012

Detail Information

Publications74

  1. pmc Pathogenesis of Helicobacter pylori infection
    Johannes G Kusters
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Clin Microbiol Rev 19:449-90. 2006
    ..This review focuses on the microbiological, clinical, immunological, and biochemical aspects of the pathogenesis of H. pylori...
  2. doi request reprint Aneuploidy and overexpression of Ki67 and p53 as markers for neoplastic progression in Barrett's esophagus: a case-control study
    Marjolein Sikkema
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Am J Gastroenterol 104:2673-80. 2009
    ..The aim of this study was to determine the predictive value of p53, Ki67, and aneuploidy as markers of neoplastic progression in BE...
  3. pmc Iron-responsive regulation of the Helicobacter pylori iron-cofactored superoxide dismutase SodB is mediated by Fur
    Florian D Ernst
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dijkzigt L 4, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Bacteriol 187:3687-92. 2005
    ..In conclusion, H. pylori Fur is a versatile regulator involved in many pathways essential for gastric colonization, including superoxide stress defense...
  4. pmc Iron-responsive repression of urease expression in Helicobacter hepaticus is mediated by the transcriptional regulator Fur
    Clara Belzer
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, 3015 CE Rotterdam, The Netherlands
    Infect Immun 75:745-52. 2007
    ..This represents a novel type of urease regulation in ureolytic bacteria and extends the already diverse regulatory repertoire of the Fur protein...
  5. ncbi request reprint Inhibition of viral replication reduces regulatory T cells and enhances the antiviral immune response in chronic hepatitis B
    Jeroen N Stoop
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, s Gravendijkwal 230, Room Ca 326, 3015 CE Rotterdam, The Netherlands
    Virology 361:141-8. 2007
    ..The production of IL10 did not decrease during therapy. In conclusion, adefovir induced viral load reduction results in a decline of circulating Treg together with a partial recovery of the immune response...
  6. pmc The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori
    Florian D Ernst
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Infect Immun 73:7252-8. 2005
    ..In conclusion, the NikR protein is able to function both as a repressor and as an activator of gene transcription, depending on the position of the binding site...
  7. pmc Acid-responsive gene induction of ammonia-producing enzymes in Helicobacter pylori is mediated via a metal-responsive repressor cascade
    Arnoud H M van Vliet
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Infect Immun 72:766-73. 2004
    ..5 in wild-type H. pylori. We hypothesize that H. pylori uses a repressor cascade to respond to low pH, with NikR initiating the response directly via the urease operon and indirectly via the members of the Fur regulon...
  8. ncbi request reprint The functional status of the Helicobacter pylori sabB adhesin gene as a putative marker for disease outcome
    Ramon de Jonge
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Helicobacter 9:158-64. 2004
    ..We have evaluated the correlation between the expression status of these four genes and disease outcome of H. pylori infection in a Dutch patient population...
  9. ncbi request reprint Transcriptional profiling of Helicobacter pylori Fur- and iron-regulated gene expression
    Florian D Ernst
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Dijkzigt L 455, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbiology 151:533-46. 2005
    ..pylori Fur is a versatile regulator involved in many pathways essential for gastric colonization. These findings further delineate the central role of Fur in regulating the unique capacity of H. pylori to colonize the human stomach...
  10. doi request reprint Myo9B is associated with an increased risk of Barrett's esophagus and esophageal adenocarcinoma
    Vivianda Menke
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Scand J Gastroenterol 47:1422-8. 2012
    ..Polymorphisms in the 3' region of myosin IXB (Myo9B) are associated with chronic inflammatory gastrointestinal disorders like celiac disease and ulcerative colitis, assuming that variation in Myo9B influences the intestinal permeability...
  11. pmc Expression, localization and polymorphisms of the nuclear receptor PXR in Barrett's esophagus and esophageal adenocarcinoma
    Anouk van de Winkel
    Department of Gastroenterology and Hepatology, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    BMC Gastroenterol 11:108. 2011
    ..This study aimed to investigate the expression and genetic variation of PXR in reflux esophagitis (RE), Barrett's esophagus (BE) and esophageal adenocarcinoma...
  12. pmc NikR mediates nickel-responsive transcriptional repression of the Helicobacter pylori outer membrane proteins FecA3 (HP1400) and FrpB4 (HP1512)
    Florian D Ernst
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Room L 455, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Infect Immun 74:6821-8. 2006
    ..pylori to the gastric lifestyle...
  13. ncbi request reprint Differential regulation of amidase- and formamidase-mediated ammonia production by the Helicobacter pylori fur repressor
    Arnoud H M van Vliet
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    J Biol Chem 278:9052-7. 2003
    ..These roles of Fur in regulation of the H. pylori amidases suggest that the H. pylori Fur regulator may have acquired extra functions to compensate for the absence of other regulatory systems...
  14. doi request reprint NcoI TNF-β gene polymorphism and TNF expression are associated with an increased risk of developing Barrett's esophagus and esophageal adenocarcinoma
    Vivianda Menke
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Scand J Gastroenterol 47:378-86. 2012
    ..The aim of the study was to determine the impact of TNF gene variation on the RE-BE-EAC cascade...
  15. ncbi request reprint UreA2B2: a second urease system in the gastric pathogen Helicobacter felis
    Raymond G J Pot
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 50:273-9. 2007
    ..Although the exact function of the UreA2B2 system is still unknown, it is conceivable that UreA2B2 may contribute to pathogenesis of H. felis infection through a yet unknown mechanism...
  16. doi request reprint The Helicobacter hepaticus hefA gene is involved in resistance to amoxicillin
    Clara Belzer
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Helicobacter 14:72-9. 2009
    ..In this study we have determined the susceptibility of gastric and enterohepatic Helicobacter species to amoxicillin, and have investigated the mechanism of amoxicillin resistance in Helicobacter hepaticus...
  17. ncbi request reprint Favorable effect of adefovir on the number and functionality of myeloid dendritic cells of patients with chronic HBV
    Renate G van der Molen
    Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Hepatology 44:907-14. 2006
    ..Our findings provide clues for the reasons why current antiviral therapy does not lead to consistently sustained viral eradication...
  18. doi request reprint Inverse nickel-responsive regulation of two urease enzymes in the gastric pathogen Helicobacter mustelae
    Jeroen Stoof
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Environ Microbiol 10:2586-97. 2008
    ..Pathogens often express colonization factors which are adapted to their host. The UreA2B2 urease could represent an example of pathogen adaptation to the specifics of the diet of their carnivorous host, rather than to the host itself...
  19. doi request reprint Bile acid-stimulated expression of the farnesoid X receptor enhances the immune response in Barrett esophagus
    Astrid Capello
    Department of Gastroenterology and Hepatology, Erasmus Universiry Medical Center, Rotterdam, The Netherlands
    Am J Gastroenterol 103:1510-6. 2008
    ..FXR activation is also involved in the induction of the innate immune response. This suggests that FXR is involved in the pathogenesis and the inflammation seen in BE...
  20. ncbi request reprint The Helicobacter pylori plasticity region locus jhp0947-jhp0949 is associated with duodenal ulcer disease and interleukin-12 production in monocyte cells
    Ramon de Jonge
    Department of Gastroenterology and Hepatology, Room L 459, Erasmus MC University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 41:161-7. 2004
    ..027). Therefore, the jhp0947-jhp0949 locus may be a novel putative H. pylori marker for disease outcome independent of the cag PAI...
  21. ncbi request reprint Tumor necrosis factor alpha inhibits the suppressive effect of regulatory T cells on the hepatitis B virus-specific immune response
    Jeroen N Stoop
    Department of Gastroenterology and Hepatology, Erasmus MC Rotterdam, The Netherlands
    Hepatology 46:699-705. 2007
    ..Because chronic HBV patients are known to produce less TNF-alpha, these data implicate an important role for TNF-alpha in the impaired antiviral response in chronic HBV...
  22. ncbi request reprint Induction of regulatory T-cells and interleukin-10-producing cells in non-responders to pegylated interferon-alpha therapy for chronic hepatitis B
    Dave Sprengers
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Centre, Rotterdam, The Netherlands
    Antivir Ther 12:1087-96. 2007
    ..We aimed to investigate whether in non-responders to IFN-alpha therapy Treg contribute to treatment failure by downregulating the HBV-specific T-cell responses...
  23. ncbi request reprint Multiple mutations in or adjacent to the conserved penicillin-binding protein motifs of the penicillin-binding protein 1A confer amoxicillin resistance to Helicobacter pylori
    Monique M Gerrits
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Helicobacter 11:181-7. 2006
    ..pylori isolates, and is mediated by mutations in penicillin-binding protein 1A (PBP1A). In this study the molecular mechanism underlying amoxicillin resistance of seven additional Amx H. pylori isolates has been established...
  24. ncbi request reprint Role of the rdxA and frxA genes in oxygen-dependent metronidazole resistance of Helicobacter pylori
    Monique M Gerrits
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    J Med Microbiol 53:1123-8. 2004
    ..As there were no significant differences in cell viability between the clinical isolates, it is likely that neither the rdxA nor the frxA gene participates in the reversibility of metronidazole resistance...
  25. ncbi request reprint Aneuploidy and high expression of p53 and Ki67 is associated with neoplastic progression in Barrett esophagus
    Marjon Kerkhof
    Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Cancer Biomark 4:1-10. 2008
    ..Accumulation of p53 was seen several years before development of HGD/EAC, and may therefore be an early marker in BE at a stage when dysplasia is not yet detected. A prospective follow-up study is needed to confirm these findings...
  26. ncbi request reprint Differential regulation of urease activity in Helicobacter hepaticus and Helicobacter pylori
    Clara Belzer
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Microbiology 151:3989-95. 2005
    ..In conclusion, both the role of the urease enzyme and the regulation of urease activity differ between the enterohepatic pathogen H. hepaticus and the gastric pathogen H. pylori...
  27. ncbi request reprint Role of the Helicobacter pylori outer-membrane proteins AlpA and AlpB in colonization of the guinea pig stomach
    Ramon de Jonge
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Med Microbiol 53:375-9. 2004
    ..05). In conclusion, absence of AlpA or AlpB is a serious disadvantage for colonization of the stomach by H. pylori...
  28. pmc Transcriptional phase variation of a type III restriction-modification system in Helicobacter pylori
    Nicolette de Vries
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Bacteriol 184:6615-23. 2002
    ..Moreover, the C tract was required for the occurrence of transcriptional phase variation. Our finding that translation and transcription are linked during phase variation through slipped-strand mispairing is new for H. pylori...
  29. ncbi request reprint COX-2 CA-haplotype is a risk factor for the development of esophageal adenocarcinoma
    Leon M G Moons
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Am J Gastroenterol 102:2373-9. 2007
    ..Neoplastic progression of BE towards EAC is associated with increased expression of COX-2. Increased COX-2 expression and enzyme activity is linked to the COX-2 CA haplotype, which consists of two gene polymorphisms in the COX-2 promoter...
  30. pmc Biomarker-based prediction of inflammatory bowel disease-related colorectal cancer: a case-control study
    Monique M Gerrits
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Cell Oncol (Dordr) 34:107-17. 2011
    ..Screening accuracy may increase by using a biomarker-based surveillance strategy...
  31. ncbi request reprint The role of the Ferric Uptake Regulator (Fur) in regulation of Helicobacter pylori iron uptake
    Arnoud H M van Vliet
    Department of Gastroenterology and Hepatology, Erasmus MC, Rotterdam, The Netherlands
    Helicobacter 7:237-44. 2002
    ..Bacterial iron-responsive regulation is often mediated by Ferric Uptake Regulator (Fur) homologs, and in this study we have determined the role of H. pylori Fur in regulation of H. pylori iron uptake...
  32. ncbi request reprint Barrett's oesophagus is characterized by a predominantly humoral inflammatory response
    Leon M G Moons
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Centre, Rotterdam, The Netherlands
    J Pathol 207:269-76. 2005
    ..It may be that this shift, which is similar to that found in other chronic inflammatory conditions, contributes to an increased cancer risk in BO...
  33. ncbi request reprint High-grade dysplasia in Barrett's esophagus is associated with increased expression of calgranulin A and B
    Dorine A Bax
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Scand J Gastroenterol 42:902-10. 2007
    ..The aim of this study was to increase the understanding of the development of dysplastic lesions in BE through the identification of genes that are differentially transcribed in these tissue types...
  34. ncbi request reprint Biomarkers for risk stratification of neoplastic progression in Barrett esophagus
    Marjon Kerkhof
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Cell Oncol 29:507-17. 2007
    ..e. proliferation/cell cycle proteins, tumor suppressor genes, adhesion molecules, DNA ploidy status and inflammation associated markers, that can be used for risk stratification in BE, and discuss their respective clinical application...
  35. ncbi request reprint NikR-mediated regulation of Helicobacter pylori acid adaptation
    Arnoud H M van Vliet
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Trends Microbiol 12:489-94. 2004
    ..Despite the paucity of regulatory proteins, this enables H. pylori to optimally adapt to conditions in the stomach, making it one of the most successful human pathogens...
  36. ncbi request reprint The stress-induced hsp12 gene shows genetic variation among Helicobacter pylori strains
    Nicolette de Vries
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 38:45-51. 2003
    ..The stress-regulated, strain-specific hsp12 genes may be involved in adaptation of individual H. pylori strains to their specific hosts, and contribute to long-term colonization of the gastric niche...
  37. ncbi request reprint Analysis of intrahepatic HBV-specific cytotoxic T-cells during and after acute HBV infection in humans
    Dave Sprengers
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Hepatol 45:182-9. 2006
    ..In this study we aimed to characterize the virus-specific CD8 + T-cell response in the liver of patients with acute HBV infection using fine-needle aspiration-biopsy (FNAB)...
  38. pmc Effects of 16S rRNA gene mutations on tetracycline resistance in Helicobacter pylori
    Monique M Gerrits
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Antimicrob Agents Chemother 47:2984-6. 2003
    ..pylori isolates...
  39. ncbi request reprint Characterization of human liver dendritic cells in liver grafts and perfusates
    Brenda M Bosma
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Liver Transpl 12:384-93. 2006
    ..These viable MDC have the capacity to stimulate allogeneic T-cells, and thus may represent a major player in the induction of acute rejection...
  40. ncbi request reprint Molecular alterations during development of esophageal adenocarcinoma
    Dorine A Bax
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    J Surg Oncol 92:89-98; discussion 99. 2005
    ..These novel developments will open new perspectives for diagnosis, treatment, and prevention of esophageal adenocarcinoma...
  41. ncbi request reprint Direct random insertion mutagenesis of Helicobacter pylori
    Ramon de Jonge
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Microbiol Methods 52:93-100. 2003
    ..The isolation of two independent H. pylori mutants defective in production of urease from this library underlines the potential of this mutagenesis strategy...
  42. ncbi request reprint The intrahepatic immune response during chronic hepatitis B infection can be monitored by the fine-needle aspiration biopsy technique
    Thjon J Tang
    Department of Gastroenterology and Hepatology, Room L 455, Erasmus MC, University Medical Center Rotterdam, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 39:69-72. 2003
    ..In conclusion, the FNAB technique is suited to investigate the intrahepatic immune response during chronic hepatitis B infection on a frequent basis...
  43. ncbi request reprint Intrahepatic CD8 T-lymphocytes and HBV core expression in relation to response to antiviral therapy for chronic hepatitis B patients
    Thjon J Tang
    Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center Rotterdam, The Netherlands
    J Med Virol 72:215-22. 2004
    ..Response to therapy led to a significant reduction of intrahepatic CD8 T-lymphocytes. Co-localisation of CD8 T-lymphocytes and HBcAg-positive hepatocytes suggests antiviral activity predominantly at the site of maximum HBV replication...
  44. pmc 16S rRNA mutation-mediated tetracycline resistance in Helicobacter pylori
    Monique M Gerrits
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands
    Antimicrob Agents Chemother 46:2996-3000. 2002
    ..This triple-base-pair substitution, present in both copies of the 16S rRNA gene of all our Tet(r) H. pylori transformants, resulted in an increased MIC of tetracycline that was identical to that for the Tet(r) strain 181...
  45. ncbi request reprint Functional impairment of myeloid and plasmacytoid dendritic cells of patients with chronic hepatitis B
    Renate G van der Molen
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Hepatology 40:738-46. 2004
    ..In conclusion, mDC and pDC are functionally impaired in patients with chronic hepatitis B. This might be an important way by which hepatitis B virus evades an adequate immune response, leading to viral persistence and disease chronicity...
  46. ncbi request reprint Detection of identical Helicobacter DNA in the stomach and in the non-cirrhotic liver of patients with hepatocellular carcinoma
    Cornelis Verhoef
    Departments of Surgical Oncology Erasmus Medical Centre, University Hospital Rotterdam, Rotterdam, The Netherlands
    Eur J Gastroenterol Hepatol 15:1171-4. 2003
    ..The possible relationship between the presence of Helicobacter species in the non-cirrhotic liver of patients with hepatocellular carcinoma (HCC) and the detection of Helicobacter species in their stomach was explored...
  47. ncbi request reprint No association between tumor necrosis factor-alpha production and gene polymorphisms among inbred rat strains
    Michiel C Warlé
    Department of Surgery, Erasmus MC Rotterdam, P O Box 2040, 3000 CA, Rotterdam, The Netherlands
    Transpl Immunol 14:77-82. 2005
    ..Without an established relationship between polymorphisms and expression of the TNF-alpha gene, it is preliminary to address a possible association of Tnf gene polymorphisms with rat liver allograft survival...
  48. ncbi request reprint Serum- and animal tissue-free medium for transport and growth of Helicobacter pylori
    Cindy M Dierikx
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    FEMS Immunol Med Microbiol 50:239-43. 2007
    ..pylori. The formulation of SATFM may allow its use in international transport of H. pylori, and may also allow certified use in immunization studies requiring growth of H. pylori and other bacterial pathogens...
  49. ncbi request reprint Immunomagnetic selection of functional dendritic cells from human lymph nodes
    Patrick P C Boor
    Department of Gastroenterology and Hepatology, Room L 455, Erasmus Medical Center, Rotterdam, The Netherlands
    Immunol Lett 99:162-8. 2005
    ..Moreover, these MDC are functionally intact. Selection with anti-BDCA-4 mAb does enrich PDC from human LN, but the resulting preparations are contaminated with T- and NK-cells...
  50. ncbi request reprint Intrahepatic CD8+ T-lymphocyte response is important for therapy-induced viral clearance in chronic hepatitis B infection
    Thjon J Tang
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, Room L 455, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    J Hepatol 43:45-52. 2005
    ..To determine which immune cells contribute to HBV-clearance during antiviral therapy, we performed a longitudinal analysis of intrahepatic immune cells during interferon-alpha therapy of chronic HBV-patients using the FNAB technique...
  51. ncbi request reprint Regulatory T cells contribute to the impaired immune response in patients with chronic hepatitis B virus infection
    Jeroen N Stoop
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands
    Hepatology 41:771-8. 2005
    ..T(reg) have an immunosuppressive effect on HBV-specific T helper cells. The presence of HBV-specific T(reg) could contribute to an inadequate immune response against the virus, leading to chronic infection...
  52. pmc NikR mediates nickel-responsive transcriptional induction of urease expression in Helicobacter pylori
    Arnoud H M van Vliet
    Department of Gastroenterology and Hepatology, Erasmus Medical Center, Rotterdam, The Netherlands
    Infect Immun 70:2846-52. 2002
    ..In conclusion, the H. pylori NikR protein and a 19-bp operator sequence in the ureA promoter are both essential for nickel-responsive induction of urease expression in H. pylori...
  53. ncbi request reprint Helicobacter species are not detectable by 16S rDNA PCR in bile from Dutch patients with common bile duct stones
    Robert Roosendaal
    Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands
    Digestion 66:89-91. 2002
    ..To explore the possible relation between Helicobacter spp. and gallbladder disorders, we have investigated their presence in bile of patients with biliary obstruction and dilatation of the bile ducts...
  54. ncbi request reprint Detection of Helicobacter pylori in bile of cats
    Sacha Y Boomkens
    Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 8, P O Box 80154, 3508 TD Utrecht, The Netherlands
    FEMS Immunol Med Microbiol 42:307-11. 2004
    ..44). Sequence analysis of the amplicons revealed a 100% identity with the Helicobacter pylori specific DNA fragments. Our data suggest an etiological role of H. pylori in feline LC and that cats are a potential zoonotic reservoir...
  55. ncbi request reprint Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLP
    Marcelo L Ribeiro
    Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Braganca Paulista, SP, Brazil
    FEMS Immunol Med Microbiol 40:57-61. 2004
    ..This PCR-RFLP-based approach distinguishes the high-level Tet(R) isolates from low-level Tet(R) and Tet(S) H. pylori strains and thus allows the direct detection of Tet(R) H. pylori isolates...
  56. ncbi request reprint Strain-specific in vitro cytokine production profiles do not predict rat liver allograft survival
    Michiel C Warlé
    Department of Surgery, Erasmus MC Rotterdam, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Transpl Int 17:779-86. 2005
    ....
  57. ncbi request reprint Induced Helicobacter pylori vacuolating cytotoxin VacA expression after initial colonisation of human gastric epithelial cells
    Karin van Amsterdam
    Academic Medical Center, Department of Medical Microbiology, P O Box 22660, 1100 DD Amsterdam, The Netherlands
    FEMS Immunol Med Microbiol 39:251-6. 2003
    ..These results show the applicability of this technique to study H. pylori gene regulation in its natural environment...
  58. ncbi request reprint Colonization with cagA-positive Helicobacter pylori strains in intestinal metaplasia of the esophagus and the esophagogastric junction
    Pernilla Ackermark
    Department of Gastroenterology, St Antonius Hospital, Nieuwegein, The Netherlands
    Am J Gastroenterol 98:1719-24. 2003
    ..pylori virulence factor cagA in it has not been investigated. The aim of our study was to assess the relationship between H. pylori and cagA-positive H. pylori in particular and IM at an endoscopically normal EGJ and Barrett's esophagus...
  59. pmc Essential role of ferritin Pfr in Helicobacter pylori iron metabolism and gastric colonization
    Barbara Waidner
    Institute of Medical Microbiology and Hygiene, Department of Medical Microbiology and Hygiene, University Hospital of Freiburg, Germany
    Infect Immun 70:3923-9. 2002
    ..In summary, the pfr phenotypes observed give new insights into prokaryotic ferritin functions and indicate that iron storage and homeostasis are of extraordinary importance for H. pylori to survive in its hostile natural environment...
  60. ncbi request reprint The mouse colonizing Helicobacter pylori strain SS1 may lack a functional cag pathogenicity island
    Jean E Crabtree
    Helicobacter 7:139-40; author reply 140-1. 2002
  61. ncbi request reprint Natural transformation in Helicobacter pylori: DNA transport in an unexpected way
    Leonard C Smeets
    Dept of Medical Microbiology and Infection Control, VU University Medical Center, PO Box 7057, 1007 MB, Amsterdam, The Netherlands
    Trends Microbiol 10:159-62; discussion 162. 2002
    ..Instead, in H. pylori it has been suggested that the five proteins that form the transmembrane channel of the transformation system are closely related to subunits of type IV secretion systems...
  62. ncbi request reprint Novel plasmids for gene expression analysis and for genetic manipulation in the gastric pathogen Helicobacter pylori
    Stefan Bereswill
    Department of Microbiology and Hygiene, National Reference Center for Helicobacter pylori, Institute of Medical Microbiology and Hygiene, University Hospital of Freiburg, Hermann Herder Strasse 11, D 79104 Freiburg, Germany
    FEMS Immunol Med Microbiol 44:157-62. 2005
    ..pylori, and may also be of use for other bacteria, as the resistance cassettes and the lacZ gene are active in the related Campylobacter species...
  63. ncbi request reprint Restriction of DNA encoding selectable markers decreases the transformation efficiency of Helicobacter pylori
    Rebecca J Gorrell
    Department of Microbiology and Immunology, University of Melbourne, Parkville VIC 3010, Australia Microbiological Research Unit, Murdoch Childrens Research Institute, Parkville VIC 3052, Australia
    FEMS Immunol Med Microbiol 44:213-9. 2005
    ..Digestion of 32P-labelled cassettes by H. pylori lysates mirrored the transformation results and indicated that in some strains these cassettes are the targets of enzymatic restriction...
  64. pmc The novel Helicobacter pylori CznABC metal efflux pump is required for cadmium, zinc, and nickel resistance, urease modulation, and gastric colonization
    Frank Nils Stähler
    Department of Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann Herder Str 11, D 79104 Freiburg, Germany
    Infect Immun 74:3845-52. 2006
    ..This demonstrates that the metal export functions of H. pylori cznABC are essential for gastric colonization and underlines the extraordinary importance of metal ion homeostasis for the survival of H. pylori in the gastric environment...
  65. ncbi request reprint Low circulating regulatory T-cell levels after acute rejection in liver transplantation
    Ahmet Demirkiran
    Department of Surgery, Erasmus MC University Medical Center, Rotterdam, The Netherlands
    Liver Transpl 12:277-84. 2006
    ..Despite these quantitative differences between rejectors and nonrejectors, the suppressive quality of CD4+CD25+ Tregs is identical in both groups...
  66. ncbi request reprint Of microbe and man: determinants of Helicobacter pylori-related diseases
    Karin van Amsterdam
    Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands
    FEMS Microbiol Rev 30:131-56. 2006
    ..In this review, we discuss host, environmental and bacterial factors that are important in disease progression. Moreover, H. pylori adaptive mechanisms, which allow its life-long survival and growth in the gastric mucosa are considered...
  67. ncbi request reprint IL-1 RN polymorphism is not associated with Barrett's esophagus and esophageal adenocarcinoma
    Leon M G Moons
    Am J Gastroenterol 100:2818. 2005
  68. ncbi request reprint Helicobacter pylori HopH (OipA) and bacterial pathogenicity: genetic and functional genomic analysis of hopH gene polymorphisms
    Anar Dossumbekova
    Second Department of Internal Medicine and Gastroenterology, Technical University of Munich, Munich, Germany
    J Infect Dis 194:1346-55. 2006
    ..Expression of the Helicobacter pylori outer membrane protein HopH is regulated by phase variation within a CT dinucleotide repeat motif of the hopH gene...
  69. pmc Real-time PCR screening for 16S rRNA mutations associated with resistance to tetracycline in Helicobacter pylori
    Erik Glocker
    Nationales Referenzzentrum für Helicobacter pylori, Abteilung Medizinische Mikrobiologie und Hygiene, Institut fur Medizinische Mikrobiologie und Hygiene, Universitatsklinikum Freiburg, Hermann Herder Strasse 11, D 79104 Freiburg, Germany
    Antimicrob Agents Chemother 49:3166-70. 2005
    ..pylori-infected gastric biopsy samples. We therefore conclude that this real-time PCR is an excellent method for determination of H. pylori tetracycline resistance even when live bacteria are no longer available...
  70. ncbi request reprint The presence of the cag pathogenicity island is associated with increased superoxide anion radical scavenging activity by Helicobacter pylori
    Jacob J Briedé
    Department of Health Risk Analysis and Toxicology, Faculty of Health Sciences, Maastricht University, Maastricht, The Netherlands
    FEMS Immunol Med Microbiol 44:227-32. 2005
    ..We hypothesize that the increased scavenging activity of cag-positive H. pylori strains is an adaptation to the increased inflammatory response associated with the cag-positive genotype of H. pylori...
  71. ncbi request reprint Random mutagenesis to identify novel Helicobacter mustelae virulence factors
    Tadhg O Cróinín
    Children s Research Centre, Our Lady s Hospital for Sick Children, Crumlin, Dublin, Ireland
    FEMS Immunol Med Microbiol 50:257-63. 2007
    ..The development of a mutant library for a natural animal model of Helicobacter infection provides the opportunity to study in vivo the role of candidate Helicobacter virulence genes...
  72. ncbi request reprint Differentially expressed genes in response to amoxicillin in Helicobacter pylori analyzed by RNA arbitrarily primed PCR
    Anita P O Godoy
    Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Braganca Paulista, SP, Brazil
    FEMS Immunol Med Microbiol 50:226-30. 2007
    ..Further analysis of these cDNAs will allow a better comprehension of both the molecular mechanism(s) of amoxicillin resistance and the adaptative mechanism(s) used by H. pylori in the presence of this antibiotic...
  73. ncbi request reprint The role of Helicobacter spp. in the pathogenesis of primary biliary cirrhosis and primary sclerosing cholangitis
    Sacha Y Boomkens
    Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    FEMS Immunol Med Microbiol 44:221-5. 2005
    ..Sequence analysis confirmed Helicobacter spp. DNA. Because Helicobacter spp. DNA can be found in approximately one-third of all samples tested, it is unlikely that PSC and PBC are caused by Helicobacter infection...
  74. pmc The Helicobacter pylori homologue of the ferric uptake regulator is involved in acid resistance
    Jetta J E Bijlsma
    Department of Medical Microbiology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands
    Infect Immun 70:606-11. 2002
    ..From this we conclude that Fur is involved in the growth at acidic pH of H. pylori; as such, it is the first regulatory protein implicated in the acid resistance of this important human pathogen...