Roland Kanaar

Summary

Affiliation: Erasmus MC
Country: The Netherlands

Publications

  1. ncbi request reprint The CSB protein actively wraps DNA
    Nancy Beerens
    Department of Cell Biology and Genetics and Radiation Oncology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Biol Chem 280:4722-9. 2005
  2. doi request reprint Targeting homologous recombination-mediated DNA repair in cancer
    João Fs Carvalho
    Erasmus MC Cancer Institute, Department of Genetics, Department of Radiation Oncology, Cancer Genomics Netherlands, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Expert Opin Ther Targets 18:427-58. 2014
  3. pmc Quality control of DNA break metabolism: in the 'end', it's a good thing
    Roland Kanaar
    Department of Cell Biology and Genetics, Erasmus Medical Center, Rotterdam, The Netherlands
    EMBO J 27:581-8. 2008
  4. ncbi request reprint DNA double-strand break repair: all's well that ends well
    Claire Wyman
    Department of Cell Biology and Genetics and Department of Radiation Oncology, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    Annu Rev Genet 40:363-83. 2006
  5. ncbi request reprint The structure-specific endonuclease Mus81 contributes to replication restart by generating double-strand DNA breaks
    Katsuhiro Hanada
    Department of Cell Biology and Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Struct Mol Biol 14:1096-104. 2007
  6. pmc The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaks
    Katsuhiro Hanada
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 25:4921-32. 2006
  7. pmc Nuclear dynamics of PCNA in DNA replication and repair
    Jeroen Essers
    Dept of Cell Biology and Genetics, Erasmus MC, Dr Molenwaterplein 50, PO Box 1738, 2015 GE Rotterdam, The Netherlands
    Mol Cell Biol 25:9350-9. 2005
  8. pmc NBS1 cooperates with homologous recombination to counteract chromosome breakage during replication
    Linda Brugmans
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    DNA Repair (Amst) 8:1363-70. 2009
  9. doi request reprint The response of mammalian cells to UV-light reveals Rad54-dependent and independent pathways of homologous recombination
    Berina Eppink
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    DNA Repair (Amst) 10:1095-105. 2011
  10. pmc Mutation of the mouse Rad17 gene leads to embryonic lethality and reveals a role in DNA damage-dependent recombination
    Magda Budzowska
    MGC Department of Cell Biology and Genetics, Center for Biomedical Genetics, Erasmus MC, DR Rotterdam, The Netherlands
    EMBO J 23:3548-58. 2004

Detail Information

Publications77

  1. ncbi request reprint The CSB protein actively wraps DNA
    Nancy Beerens
    Department of Cell Biology and Genetics and Radiation Oncology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Biol Chem 280:4722-9. 2005
    ..We also provide evidence suggesting that CSB binds DNA as a dimer. DNA wrapping and unwrapping allows CSB to actively alter the DNA double helix conformation, which could influence nucleosomes and other protein-DNA interactions...
  2. doi request reprint Targeting homologous recombination-mediated DNA repair in cancer
    João Fs Carvalho
    Erasmus MC Cancer Institute, Department of Genetics, Department of Radiation Oncology, Cancer Genomics Netherlands, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Expert Opin Ther Targets 18:427-58. 2014
    ....
  3. pmc Quality control of DNA break metabolism: in the 'end', it's a good thing
    Roland Kanaar
    Department of Cell Biology and Genetics, Erasmus Medical Center, Rotterdam, The Netherlands
    EMBO J 27:581-8. 2008
    ..Thus, paradoxically, enzymes antagonizing DNA end metabolism help to ensure that genome maintenance becomes a robust process...
  4. ncbi request reprint DNA double-strand break repair: all's well that ends well
    Claire Wyman
    Department of Cell Biology and Genetics and Department of Radiation Oncology, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    Annu Rev Genet 40:363-83. 2006
    ....
  5. ncbi request reprint The structure-specific endonuclease Mus81 contributes to replication restart by generating double-strand DNA breaks
    Katsuhiro Hanada
    Department of Cell Biology and Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Nat Struct Mol Biol 14:1096-104. 2007
    ..We suggest that Mus81 suppresses chromosomal instability by converting potentially detrimental replication-associated DNA structures into intermediates that are more amenable to DNA repair...
  6. pmc The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaks
    Katsuhiro Hanada
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 25:4921-32. 2006
    ..We propose that Mus81-Eme1- and Rad54-mediated homologous recombination are involved in the same DNA replication-dependent ICL repair pathway...
  7. pmc Nuclear dynamics of PCNA in DNA replication and repair
    Jeroen Essers
    Dept of Cell Biology and Genetics, Erasmus MC, Dr Molenwaterplein 50, PO Box 1738, 2015 GE Rotterdam, The Netherlands
    Mol Cell Biol 25:9350-9. 2005
    ..Our results show that changes in the position of PCNA result from de novo assembly of freely mobile replication factors in the nucleoplasmic pool and indicate different binding affinities for PCNA in DNA replication and repair...
  8. pmc NBS1 cooperates with homologous recombination to counteract chromosome breakage during replication
    Linda Brugmans
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    DNA Repair (Amst) 8:1363-70. 2009
    ..We conclude that Nbs1 and Rad54 function cooperatively, but in separate pathways to counteract this type of DNA damage and discuss mechanistic implications of these findings...
  9. doi request reprint The response of mammalian cells to UV-light reveals Rad54-dependent and independent pathways of homologous recombination
    Berina Eppink
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    DNA Repair (Amst) 10:1095-105. 2011
    ..In conclusion, we show that HR is important for the progression of UV-stalled replication forks in ES cells, and that protection of the fork is an interplay between HR and TLS...
  10. pmc Mutation of the mouse Rad17 gene leads to embryonic lethality and reveals a role in DNA damage-dependent recombination
    Magda Budzowska
    MGC Department of Cell Biology and Genetics, Center for Biomedical Genetics, Erasmus MC, DR Rotterdam, The Netherlands
    EMBO J 23:3548-58. 2004
    ....
  11. pmc Dynamics of relative chromosome position during the cell cycle
    Jeroen Essers
    Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Mol Biol Cell 16:769-75. 2005
    ..However, the daughter cells eventually regain the general chromosomal organization pattern of their mothers, suggesting an active mechanism could be at play to reestablish chromosomal neighborhoods...
  12. ncbi request reprint Analysis of ionizing radiation-induced foci of DNA damage repair proteins
    Lieneke R van Veelen
    Department of Cell Biology and Genetics, Erasmus MC, University Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Mutat Res 574:22-33. 2005
    ..Rad51 and Mre11 foci seemed to be mutually exclusive, though a small subset of cells did show co-localization of these proteins, which suggests a possible cooperation between the proteins at a specific moment during DNA repair...
  13. ncbi request reprint Cell cycle-dependent processing of DNA lesions controls localization of Rad9 to sites of genotoxic stress
    Daniel O Warmerdam
    Department of Cell Biology and Genetics, Cancer Genome Center, Erasmus MC, Rotterdam, The Netherlands
    Cell Cycle 8:1765-74. 2009
    ..These findings show that due to processing of DNA lesions into a common intermediate, which occurs in a cell cycle-dependent manner, Rad9 is able to respond to different types of genotoxic stress...
  14. ncbi request reprint Ionizing radiation-induced foci formation of mammalian Rad51 and Rad54 depends on the Rad51 paralogs, but not on Rad52
    Lieneke R van Veelen
    Department of Cell Biology and Genetics, Erasmus MC, University Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Mutat Res 574:34-49. 2005
    ..Despite their evolutionary conservation and biochemical similarities, S. cerevisiae and mammalian Rad52 appear to differentially contribute to the DNA-damage response...
  15. pmc Differential contributions of mammalian Rad54 paralogs to recombination, DNA damage repair, and meiosis
    Joanna Wesoly
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Mol Cell Biol 26:976-89. 2006
    ..Thus, even though the paralogs have similar biochemical properties, genetic analysis in mice uncovered their nonoverlapping roles...
  16. ncbi request reprint Activation of multiple DNA repair pathways by sub-nuclear damage induction methods
    Christoffel Dinant
    Department of Pathology, Josephine Nefkens Institute, ErasmusMC, Rotterdam, The Netherlands
    J Cell Sci 120:2731-40. 2007
    ..Whereas, application of low-dose 266 nm laser irradiation induced only UV-specific DNA photo-lesions allowing the study of the UV-C-induced DNA damage response in a user-defined area in cultured cells...
  17. pmc ATP-dependent and independent functions of Rad54 in genome maintenance
    Sheba Agarwal
    Department of Cell Biology and Genetics, Cancer Genomics Center, 3000 CA Rotterdam, Netherlands
    J Cell Biol 192:735-50. 2011
    ..Finally, we show that ATP hydrolysis by Rad54 is required for the redistribution of DSB repair sites within the nucleus...
  18. pmc Rad52 and Ku bind to different DNA structures produced early in double-strand break repair
    Dejan Ristic
    Department of Cell Biology and Genetics, Erasmus MC, The Netherlands
    Nucleic Acids Res 31:5229-37. 2003
    ..Rather, they interact with different DNA substrates produced early in DNA double-strand break repair...
  19. pmc Human Rad51 filaments on double- and single-stranded DNA: correlating regular and irregular forms with recombination function
    Dejan Ristic
    Department of Cell Biology and Genetics, Erasmus Medical Center PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Nucleic Acids Res 33:3292-302. 2005
    ..The dynamic rearrangements of proteins and DNA within Rad51 nucleoprotein filaments could be key events driving strand exchange in homologous recombination...
  20. ncbi request reprint Fluorescent human RAD51 reveals multiple nucleation sites and filament segments tightly associated along a single DNA molecule
    Mauro Modesti
    Department of Cell Biology and Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Structure 15:599-609. 2007
    ..Filament segments did not rearrange along DNA, indicating tight association of the ATP-bound protein. The kinetics of filament disassembly was defined by activating ATP hydrolysis and following individual filaments in real time...
  21. ncbi request reprint RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination
    Mauro Modesti
    Department of Cell Biology and Genetics, Erasmus MC, PO Box 2040, 3000 CA, Rotterdam, The Netherlands
    Mol Cell 28:468-81. 2007
    ..The affinity of RAD51AP1 for the central protein and DNA intermediates of recombination confers on it the ability to control the preservation of genome integrity at a number of critical mechanistic steps...
  22. pmc RAD50 and NBS1 form a stable complex functional in DNA binding and tethering
    Eddy van der Linden
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Nucleic Acids Res 37:1580-8. 2009
    ..These data show that in vitro, R, M and N form a variety of stable complexes with variable subunit composition and stoichiometry, which may be physiologically relevant in different aspects of RMN function...
  23. pmc Combined optical and topographic imaging reveals different arrangements of human RAD54 with presynaptic and postsynaptic RAD51-DNA filaments
    Humberto Sanchez
    Department of Genetics, Erasmus Medical Center, Rotterdam 3000 CA, The Netherlands
    Proc Natl Acad Sci U S A 110:11385-90. 2013
    ..The different architectural arrangements we observe for RAD54 with RAD51-DNA filaments may reflect the diverse roles of this protein in homologous recombination. ..
  24. pmc Transcriptome analysis reveals cyclobutane pyrimidine dimers as a major source of UV-induced DNA breaks
    George A Garinis
    Department of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands
    EMBO J 24:3952-62. 2005
    ..Thus, conversion of unrepaired CPD lesions into DNA breaks during DNA replication may comprise one of the principal instigators of UV-mediated cytotoxicity...
  25. ncbi request reprint Analysis of DNA recombination and repair proteins in living cells by photobleaching microscopy
    Jeroen Essers
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    Methods Enzymol 408:463-85. 2006
    ....
  26. ncbi request reprint Homologous recombination-mediated double-strand break repair
    Claire Wyman
    Department of Cell Biology and Genetics, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    DNA Repair (Amst) 3:827-33. 2004
    ..The challenge ahead is to uncover the relationship between the individual biochemical activities of homologous recombination proteins and their coordinated action in the context of the living cell...
  27. ncbi request reprint Architecture of a polycomb nucleoprotein complex
    Adone Mohd-Sarip
    Department of Biochemistry, Centre for Biomedical Genetics, Erasmus University Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Mol Cell 24:91-100. 2006
    ..Indeed, chromatin immunoprecipitations (ChIPs) and nuclease mapping demonstrated that PREs are nucleosome depleted in vivo. We discuss the implications of these findings for models explaining PRE function...
  28. doi request reprint Regulation of DNA strand exchange in homologous recombination
    J Thomas Holthausen
    Department of Cell Biology and Genetics, Cancer Genomics Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    DNA Repair (Amst) 9:1264-72. 2010
    ....
  29. ncbi request reprint Perturbations of vascular homeostasis and aortic valve abnormalities in fibulin-4 deficient mice
    Katsuhiro Hanada
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    Circ Res 100:738-46. 2007
    ..Our results show that the dosage of a single gene can determine the severity of aneurysm formation and imply that disturbed TGF-beta signaling underlies multiple aneurysm phenotypes...
  30. pmc Molecular recognition of DNA-protein complexes: a straightforward method combining scanning force and fluorescence microscopy
    Humberto Sanchez
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Ultramicroscopy 110:844-51. 2010
    ..Our experiments reveal the versatility of this method for analysis of proteins and protein-DNA complexes...
  31. ncbi request reprint Homologous recombination: down to the wire
    Claire Wyman
    Department of Cell Biology and Genetics, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Biol 14:R629-31. 2004
    ..These proteins can occur in different quaternary arrangements: rings or helical filaments. Recent results reveal that recombinase function follows from the filamentous form...
  32. pmc DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP
    Martijn de Jager
    Department of Cell Biology and Genetics, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Nucleic Acids Res 30:4425-31. 2002
    ..Interestingly, ATP binding, but not hydrolysis, increased the preference of R/M binding to DNA substrates with 3'-overhangs relative to substrates with blunt ends and 5'-overhangs...
  33. ncbi request reprint Rad54, a Jack of all trades in homologous recombination
    T L Raoul Tan
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    DNA Repair (Amst) 2:787-94. 2003
    ..It plays important roles in central processes in the cell such as genome duplication and DNA damage repair. Recent experiments reveal the surprising versatility of one of its central actors, the Rad54 protein...
  34. pmc Caffeine suppresses homologous recombination through interference with RAD51-mediated joint molecule formation
    Alex N Zelensky
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands
    Nucleic Acids Res 41:6475-89. 2013
    ....
  35. doi request reprint Mechanisms of dealing with DNA damage-induced replication problems
    Magda Budzowska
    Department of Cell Biology and Genetics, Cancer Genomics Center, Rotterdam, The Netherlands
    Cell Biochem Biophys 53:17-31. 2009
    ..Homologous recombination uses homologous DNA sequence to repair or bypass the lesion and is therefore mainly error free. Error-prone translesion synthesis employs specialized, low fidelity polymerases to bypass the damage...
  36. pmc The structure-specific endonuclease Ercc1-Xpf is required to resolve DNA interstrand cross-link-induced double-strand breaks
    Laura J Niedernhofer
    Department of Cell Biology and Genetics, Erasmus Medical Center, Rotterdam, The Netherlands
    Mol Cell Biol 24:5776-87. 2004
    ..Collectively, these data support a role for Ercc1-Xpf in processing ICL-induced DSBs so that these cytotoxic intermediates can be repaired by homologous recombination...
  37. doi request reprint UV-induced G2 checkpoint depends on p38 MAPK and minimal activation of ATR-Chk1 pathway
    Daniel O Warmerdam
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
    J Cell Sci 126:1923-30. 2013
    ..UV-induced G2 checkpoint activation in this situation therefore relies on signalling via the p38 MAPK and ATR-Chk1 signalling cascades...
  38. ncbi request reprint Differential arrangements of conserved building blocks among homologs of the Rad50/Mre11 DNA repair protein complex
    Martijn de Jager
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Mol Biol 339:937-49. 2004
    ..The eukaryotic complexes were similar to each other and differed from their prokaryotic and archaeal homologs. These similarities and differences are discussed with respect to their diverse mechanistic roles in chromosome metabolism...
  39. ncbi request reprint DNA double-strand break repair and chromosome translocations
    Sheba Agarwal
    Department of Cell Biology and Genetics, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    DNA Repair (Amst) 5:1075-81. 2006
    ..Due to selection at the level of the DNA, the cell, and the tissue certain forms of cancer are associated with specific translocations that can be used as a tool for diagnosis and prognosis of these cancers...
  40. ncbi request reprint Analysis of mouse Rad54 expression and its implications for homologous recombination
    Jeroen Essers
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    DNA Repair (Amst) 1:779-93. 2002
    ..Rad54 binds duplex DNA distant from the site that will be unwound. Translocation of Rad54 along the duplex DNA increases superhelical stress thereby promoting duplex DNA unwinding...
  41. doi request reprint From DNA end chemistry to cell-cycle response: the importance of structure, even when it's broken
    Claire Wyman
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Mol Cell 30:5-6. 2008
    ..In this issue of Molecular Cell, Barlow et al. (2008) show that not all DNA double-strand breaks are processed equally and that the chemical nature of DNA ends guides different paths to DNA repair...
  42. ncbi request reprint Multiple interlinked mechanisms to circumvent DNA replication roadblocks
    Berina Eppink
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    Exp Cell Res 312:2660-5. 2006
    ..Homologous recombination, translesion DNA synthesis and de novo reinitiation of DNA synthesis ensure robust replication by navigating it passed damaged DNA. In this review, we highlight the relationship between these three processes...
  43. ncbi request reprint Analysis of DNA double-strand break repair pathways in mice
    Linda Brugmans
    Department of Cell Biology and Genetics, Erasmus MC, Dr Molewaterplein 50, PO Box 1738, Rotterdam 3015GE, The Netherlands
    Mutat Res 614:95-108. 2007
    ..In this review, we focus on the biological relevance of DSB repair in mammalian cells and the potential overlap between nonhomologous end-joining and homologous recombination in different tissues...
  44. doi request reprint Inhibition of homologous recombination by hyperthermia shunts early double strand break repair to non-homologous end-joining
    Judith W J Bergs
    Laboratory for Experimental Oncology and Radiobiology LEXOR, Center for Experimental Molecular Medicine, Department of Radiation Oncology, Academic Medical Center, University of Amsterdam, P O Box 22700, 1100 DE Amsterdam, The Netherlands
    DNA Repair (Amst) 12:38-45. 2013
    ..These results might be of consequence for clinical cancer treatment approaches that aim at inhibition of one or more DSB repair pathways...
  45. pmc Mismatch repair inhibits homeologous recombination via coordinated directional unwinding of trapped DNA structures
    Khek Chian Tham
    Department of Genetics, Cancer Genomics Netherlands, Erasmus Medical Center, Rotterdam 3000 CA, The Netherlands
    Mol Cell 51:326-37. 2013
    ..Thus, our results explain on a mechanistic level how the coordinated action between MutS, MutL, and UvrD prevents homeologous recombination and maintains genome stability...
  46. pmc Mre11-Rad50 complex crystals suggest molecular calisthenics
    Claire Wyman
    Department of Radiation Oncology, Department of Genetics and Cancer, Erasmus University Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands
    DNA Repair (Amst) 10:1066-70. 2011
    ..Recently published crystal structures of different Mre11 and Rad50 complexes show the arrangement of these proteins and imply dramatic ligand-induced rearrangements with important functional consequences...
  47. ncbi request reprint Quantitative analysis of radiation-induced DNA break repair in a cultured oral mucosal model
    Hinne A Rakhorst
    Department of Plastic, Reconstructive and Hand Surgery, Erasmus MC, Rotterdam, The Netherlands
    Tissue Eng 12:3395-403. 2006
    ..This is an important step forward in the study of mucositis and the development of treatment and prevention strategies, proving once more the power and clinical importance of tissue engineering...
  48. doi request reprint Dealing with DNA damage: relationships between checkpoint and repair pathways
    Daniel O Warmerdam
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus MC, Rotterdam, The Netherlands
    Mutat Res 704:2-11. 2010
    ..We highlight recent advances in in vivo imaging that greatly expand our knowledge on the relationships between DNA damage checkpoints and DNA repair...
  49. ncbi request reprint Tetramerization and DNA ligase IV interaction of the DNA double-strand break repair protein XRCC4 are mutually exclusive
    Mauro Modesti
    Department of Cell Biology and Genetics, Erasmus Medical Center, P O Box 1738, 3000 DR, Rotterdam, The Netherlands
    J Mol Biol 334:215-28. 2003
    ..We propose that the putative function of the XRCC4 tetramer is distinct from its DNA ligase IV-associated function...
  50. ncbi request reprint Genome instability and Rad50(S): subtle yet severe
    Martijn de Jager
    Department of Cell Biology and Genetics, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    Genes Dev 16:2173-8. 2002
  51. pmc Nuclear dynamics of RAD52 group homologous recombination proteins in response to DNA damage
    Jeroen Essers
    Department of Cell Biology and Genetics, Erasmus University Rotterdam, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    EMBO J 21:2030-7. 2002
    ..In the case of DNA repair, for example, it will facilitate cross-talk between different DNA repair pathways and coupling to other DNA transactions, such as replication...
  52. doi request reprint DNA repair by the MRN complex: break it to make it
    Roland Kanaar
    Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    Cell 135:14-6. 2008
    ..Buis et al. (2008) and Williams et al. (2008) now reveal the importance of the nuclease activity of Mre11 for mammalian genome maintenance and present a molecular view of its active site...
  53. doi request reprint Hyperthermia-induced DNA repair deficiency suggests novel therapeutic anti-cancer strategies
    Berina Eppink
    Department of Cell Biology and Genetics, Cancer Genomics Centre, Erasmus Medical Centre, Rotterdam, The Netherlands
    Int J Hyperthermia 28:509-17. 2012
    ....
  54. ncbi request reprint Chromosome Organization: Reaching out to Embrace New Models
    Claire Wyman
    Department of Cell Biology and Genetics, Erasmus Medical Center Daniel den Hoed Cancer Center, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Biol 12:R446-8. 2002
    ..New information on the architecture of protein complexes involved in the dynamic organization of chromosomes challenges existing models of their structures and provides fresh insight into their mechanisms of action...
  55. doi request reprint Homologous recombination in real time: DNA strand exchange by RecA
    Thijn van der Heijden
    Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Mol Cell 30:530-8. 2008
    ....
  56. pmc Rad54 and DNA Ligase IV cooperate to maintain mammalian chromatid stability
    Kevin D Mills
    CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    Genes Dev 18:1283-92. 2004
    ..These findings demonstrate a role for NHEJ in the repair of DSBs that occur spontaneously during or after DNA replication, and reveal overlapping functions for NHEJ and Rad54-dependent HR in the repair of such DSBs...
  57. pmc Brca2 (XRCC11) deficiency results in radioresistant DNA synthesis and a higher frequency of spontaneous deletions
    Maria Kraakman-van der Zwet
    Department of Radiation Genetics and Chemical Mutagenesis MGC, Leiden University Medical Center, The Netherlands
    Mol Cell Biol 22:669-79. 2002
    ....
  58. pmc Mammalian Rad51C contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability
    Barbara C Godthelp
    Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands
    Nucleic Acids Res 30:2172-82. 2002
    ..However, centrosome formation was not affected. Intriguingly, a reduced level of sister chromatid cohesion was found in CL-V4B cells. These results reveal a role for Rad51C that is unique among the Rad51 paralogs...
  59. pmc Evidence for replicative repair of DNA double-strand breaks leading to oncogenic translocation and gene amplification
    Michael J Difilippantonio
    Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 196:469-80. 2002
    ..Thus, mice deficient in NHEJ provide excellent models to study the etiology of unbalanced translocations and amplification events during tumorigenesis...
  60. pmc The coiled-coil of the human Rad50 DNA repair protein contains specific segments of increased flexibility
    John van Noort
    Department of Nanoscience and Delft Institute of Microelectronics and Submicrontechnology, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Proc Natl Acad Sci U S A 100:7581-6. 2003
    ..Indeed, in Rad50 coiled-coils, two positions of increased flexibility are observed. We discuss how this dynamic structural feature is integral to Rad50 function...
  61. pmc Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells
    Jacinth Abraham
    Advanced Medical Discovery Institute, Ontario Cancer Institute, 620 University Avenue, Suite 706, Toronto, Ontario M5G 2C1, Canada
    EMBO J 22:6137-47. 2003
    ..Most importantly, Eme1 deficiency led to spontaneous genomic instability. These results reveal that mammalian Eme1 plays a key role in DNA repair and the maintenance of genome integrity...
  62. ncbi request reprint Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains
    Jacob A Aten
    Center for Microscopical Research, Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, P O Box 22700, 1100 DE Amsterdam, Netherlands
    Science 303:92-5. 2004
    ..Our results support the breakage-first theory to explain the origin of chromosomal translocations...
  63. pmc Collaboration of homologous recombination and nonhomologous end-joining factors for the survival and integrity of mice and cells
    Chrystelle Couedel
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Genes Dev 18:1293-304. 2004
    ..The substantially increased DNA damage response in the double mutants implies a cooperation of the two DSB repair pathways for survival and genomic integrity in the animal...
  64. ncbi request reprint Involvement of mammalian Mus81 in genome integrity and tumor suppression
    John Peter McPherson
    Ontario Cancer Institute, 620 University Avenue, Suite 706, Toronto, Ontario, Canada M5G 2C1
    Science 304:1822-6. 2004
    ..These studies demonstrate a critical role for the proper biallelic expression of the mammalian Mus81 in the maintenance of genomic integrity and tumor suppression...
  65. ncbi request reprint Recombination mechanisms; fortieth anniversary meeting of the Holliday model
    Wolf Dietrich Heyer
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, CA 95616 USA
    Mol Cell 16:1-9. 2004
    ..In those forty years the Holliday junction has gone from a hypothetical structure to models for its atomic structure and visualization of its dynamics at the single molecule level...
  66. pmc Dissecting elastic heterogeneity along DNA molecules coated partly with Rad51 using concurrent fluorescence microscopy and optical tweezers
    Joost van Mameren
    Biophys J 91:L78-80. 2006
    ..These results illustrate the power of adding sensitive fluorescence imaging to optical tweezers instrumentation...
  67. pmc BRCTx is a novel, highly conserved RAD18-interacting protein
    David J Adams
    The Wellcome Trust Sanger Institute Hinxton, Cambs CB10 1SA, United Kingdom
    Mol Cell Biol 25:779-88. 2005
    ..We conclude that, although highly conserved, Brctx is not essential for the above-mentioned processes and may be redundant...
  68. pmc Torque-limited RecA polymerization on dsDNA
    Thijn van der Heijden
    Kavli Institute of Nanoscience, Delft University of Technology Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Nucleic Acids Res 33:2099-105. 2005
    ..We deduce a maximum built-up torsion of 10.1 +/- 0.7 k(b)T. In vivo this built-up torsion may be used to favor regression of a stalled replication fork or to free the chromosomal DNA in E.coli from its condensing proteins...
  69. ncbi request reprint Mesoscale conformational changes in the DNA-repair complex Rad50/Mre11/Nbs1 upon binding DNA
    Fernando Moreno-Herrero
    Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Nature 437:440-3. 2005
    ..The hR/M/N complex thus is an example of a biological nanomachine in which binding to its ligand, in this case DNA, affects the functional conformation of a domain located 50 nm distant...
  70. pmc Chromosomal organization: mingling with the neighbors
    Jacob A Aten
    Department of Cell Biology and Histology, Center for Microscopical Research, University of Amsterdam, Amsterdam, Netherlands
    PLoS Biol 4:e155. 2006
  71. ncbi request reprint Comment on "Direct and real-time visualization of the disassembly of a single RecA-DNA-ATPgammaS complex using AFM imaging in fluid"
    Thijn van der Heijden
    Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Nano Lett 6:3000-2. 2006
  72. pmc Real-time assembly and disassembly of human RAD51 filaments on individual DNA molecules
    Thijn van der Heijden
    Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Nucleic Acids Res 35:5646-57. 2007
    ..Instead each nucleoprotein filament consists of a string of many small filament patches that are only a few tens of monomers long. The high flexibility and dynamic nature of this arrangement is likely to facilitate strand exchange...
  73. ncbi request reprint Gene conversion-like sequence transfers between transgenic antibody V genes are independent of RAD54
    Nicole D'Avirro
    Genetics Program, Immunology Program, Sackler School of Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA
    J Immunol 169:3069-75. 2002
    ....
  74. pmc CTG repeat instability and size variation timing in DNA repair-deficient mice
    Cédric Savouret
    INSERM UR383, Hopital Necker Enfants Malades, Universite Paris V, 149 161 Rue de Sèvres, 75015 Paris, France
    EMBO J 22:2264-73. 2003
    ..We found that instability over generations depends not only on parental germinal instability, but also on a second event taking place after fertilization...
  75. pmc Suppression of retroviral infection by the RAD52 DNA repair protein
    Alan Lau
    KuDOS Pharmaceuticals Limited, 327 Cambridge Science Park, Cambridge, UK
    EMBO J 23:3421-9. 2004
    ..Instead, the mechanism of attenuation of retroviral infection by RAD52 appears to be based upon competition between the RAD52 protein and active integration complexes for the retroviral cDNA genome...
  76. ncbi request reprint Somatic hypermutation does not require Rad54 and Rad54B-mediated homologous recombination
    Linda Bross
    Research Institute Growth and Development, Department of Immunology, University of Maastricht, Maastricht, The Netherlands
    Eur J Immunol 33:352-7. 2003
    ..Thus, our data indicate that Rad54 and Rad54B-mediated HR is not essential for SHM. In addition, the finding that the ablation of RAD51 paralogues causes an increase in SHM argues against a direct involvement of HR in promoting SHM...
  77. doi request reprint Induction of linear tracks of DNA double-strand breaks by alpha-particle irradiation of cells
    Jan Stap
    Center for Microscopical Research, Department of Cell Biology and Histology, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Nat Methods 5:261-6. 2008
    ..Because the live-cell procedure requires more hands-on experience, we recommend starting with the fixed-cell application...