John P Hays

Summary

Affiliation: Erasmus MC
Country: The Netherlands

Publications

  1. ncbi request reprint High-throughput amplification fragment length polymorphism (htAFLP) analysis identifies genetic lineage markers but not complement phenotype-specific markers in Moraxella catarrhalis
    J P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 13:55-62. 2007
  2. pmc Comparative analysis and supragenome modeling of twelve Moraxella catarrhalis clinical isolates
    Jeremiah J Davie
    Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York, USA
    BMC Genomics 12:70. 2011
  3. doi request reprint The evaluation of putative endogenous control housekeeping genes for real-time polymerase chain reaction expression studies in Moraxella catarrhalis
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Diagn Microbiol Infect Dis 65:323-6. 2009
  4. ncbi request reprint Pneumococcal vaccination does not affect the genetic diversity of Moraxella catarrhalis isolates in children
    J P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 23:801-3. 2004
  5. ncbi request reprint A novel plasmid (pEMCJH03) isolated from moraxella catarrhalis possibly useful as a cloning and expression vector within this species
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC Erasmus University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Plasmid 53:263-8. 2005
  6. ncbi request reprint Moraxella catarrhalis is only a weak activator of the mannose-binding lectin (MBL) pathway of complement activation
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    FEMS Microbiol Lett 249:207-9. 2005
  7. pmc Identification and characterization of a novel outer membrane protein (OMP J) of Moraxella catarrhalis that exists in two major forms
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Bacteriol 187:7977-84. 2005
  8. pmc Comparative analysis of the humoral immune response to Moraxella catarrhalis and Streptococcus pneumoniae surface antigens in children suffering from recurrent acute otitis media and chronic otitis media with effusion
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Vaccine Immunol 19:914-8. 2012
  9. doi request reprint Analysis of VanA vancomycin-resistant Enterococcus faecium isolates from Saudi Arabian hospitals reveals the presence of clonal cluster 17 and two new Tn1546 lineage types
    Mushtaq A Khan
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015CE Rotterdam, The Netherlands
    J Antimicrob Chemother 62:279-83. 2008
  10. doi request reprint bro {beta}-lactamase and antibiotic resistances in a global cross-sectional study of Moraxella catarrhalis from children and adults
    Mushtaq A Khan
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Antimicrob Chemother 65:91-7. 2010

Detail Information

Publications20

  1. ncbi request reprint High-throughput amplification fragment length polymorphism (htAFLP) analysis identifies genetic lineage markers but not complement phenotype-specific markers in Moraxella catarrhalis
    J P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 13:55-62. 2007
    ..catarrhalis is probably defined by multiple genes, although not all of these genes may be present within all M. catarrhalis isolates...
  2. pmc Comparative analysis and supragenome modeling of twelve Moraxella catarrhalis clinical isolates
    Jeremiah J Davie
    Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York, USA
    BMC Genomics 12:70. 2011
    ..catarrhalis clinical isolates, a greater understanding of this pathogen's genome and its variability among isolates is needed...
  3. doi request reprint The evaluation of putative endogenous control housekeeping genes for real-time polymerase chain reaction expression studies in Moraxella catarrhalis
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Diagn Microbiol Infect Dis 65:323-6. 2009
    ..This study shows that a combination of the iron sequestering gene copB and 16S rRNA genes would be useful for lineage 1 (16S rRNA type 1) isolates, but not lineage 2 (16S rRNA types 2 and 3) isolates...
  4. ncbi request reprint Pneumococcal vaccination does not affect the genetic diversity of Moraxella catarrhalis isolates in children
    J P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam EMCR, Dr Molewaterplein 40, 3015 GD, Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 23:801-3. 2004
  5. ncbi request reprint A novel plasmid (pEMCJH03) isolated from moraxella catarrhalis possibly useful as a cloning and expression vector within this species
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC Erasmus University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Plasmid 53:263-8. 2005
    ..catarrhalis using pEMCJH04 could help in the development of a vaccine expression vector, as well as providing a useful tool for studying promoter activity and in complementation studies of gene knockout mutants...
  6. ncbi request reprint Moraxella catarrhalis is only a weak activator of the mannose-binding lectin (MBL) pathway of complement activation
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    FEMS Microbiol Lett 249:207-9. 2005
    ..Results showed that this organism is only a poor activator of the lectin pathway of complement activation, with subsequent consequences for the etiology of otitis media by this organism...
  7. pmc Identification and characterization of a novel outer membrane protein (OMP J) of Moraxella catarrhalis that exists in two major forms
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Bacteriol 187:7977-84. 2005
    ....
  8. pmc Comparative analysis of the humoral immune response to Moraxella catarrhalis and Streptococcus pneumoniae surface antigens in children suffering from recurrent acute otitis media and chronic otitis media with effusion
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Vaccine Immunol 19:914-8. 2012
    ..Other factors, such as Eustachian tube dysfunction, viral load, and genetic and environmental factors, may play a more important role in the pathogenesis of OM and in particular in the development of rAOM or COME...
  9. doi request reprint Analysis of VanA vancomycin-resistant Enterococcus faecium isolates from Saudi Arabian hospitals reveals the presence of clonal cluster 17 and two new Tn1546 lineage types
    Mushtaq A Khan
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015CE Rotterdam, The Netherlands
    J Antimicrob Chemother 62:279-83. 2008
    ..The aim of this study was to characterize 34 vancomycin-resistant VanA Enterococcus faecium isolates obtained from two hospitals in Saudi Arabia and to assess Tn1546 variation within these isolates...
  10. doi request reprint bro {beta}-lactamase and antibiotic resistances in a global cross-sectional study of Moraxella catarrhalis from children and adults
    Mushtaq A Khan
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Antimicrob Chemother 65:91-7. 2010
    ..To compare and contrast the geographic and demographic distribution of bro beta-lactamase and antibiotic MIC(50/90) for 1440 global Moraxella catarrhalis isolates obtained from children and adults between 2001 and 2002...
  11. ncbi request reprint Total genome polymorphism and low frequency of intra-genomic variation in the uspA1 and uspA2 genes of Moraxella catarrhalis in otitis prone and non-prone children up to 2 years of age. Consequences for vaccine design?
    John P Hays
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands
    Vaccine 21:1118-24. 2003
    ..Variation in the uspA2 5' non-coding "AGAT" repeat region was also observed. These results may have implications for future M. catarrhalis vaccines comprising UspA1 or UspA2 components...
  12. doi request reprint Temporal development of the humoral immune response to surface antigens of Moraxella catarrhalis in young infants
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    Vaccine 29:5603-10. 2011
    ..catarrhalis has not matured at 2 years of age. We provide evidence that a humoral antibody response to OMPs UspA1, UspA2 and Hag/MID may play a role in the immune response to community acquired M. catarrhalis colonization events...
  13. doi request reprint Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Microbiology 154:1178-84. 2008
    ..Our results should also be useful in the choice of effective vaccine candidates against M. catarrhalis...
  14. doi request reprint Colonization of healthy children by Moraxella catarrhalis is characterized by genotype heterogeneity, virulence gene diversity and co-colonization with Haemophilus influenzae
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Microbiology 157:169-78. 2011
    ..catarrhalis isolates, may be a result of immune evasion by this pathogen. Finally, the factors facilitating M. catarrhalis and H. influenzae co-colonization need to be further investigated...
  15. doi request reprint Determinants of Moraxella catarrhalis colonization in healthy Dutch children during the first 14 months of life
    S J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    Clin Microbiol Infect 16:992-7. 2010
    ..Apparently, crowding is an important risk factor for early and frequent colonization with M. catarrhalis in the first year of life...
  16. pmc Differential virulence gene expression of group A Streptococcus serotype M3 in response to co-culture with Moraxella catarrhalis
    Suzanne J C Verhaegh
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 8:e62549. 2013
    ..This study provides the first evidence that M. catarrhalis increases GAS virulence gene expression during co-culture, and again shows the importance of polymicrobial infections in directing bacterial virulence...
  17. doi request reprint Inflammation in the middle ear of children with recurrent or chronic otitis media is associated with bacterial load
    Kim Stol
    Department of Pediatrics, Radboud University, Nijmegen Medical Centre, The Netherlands
    Pediatr Infect Dis J 31:1128-34. 2012
    ..To clarify the role of viral infections in OM, we compared the relative contribution of viruses and bacteria with the induction of inflammatory cytokine responses in the middle ear of children suffering from OM...
  18. pmc Perceptions of point-of-care infectious disease testing among European medical personnel, point-of-care test kit manufacturers, and the general public
    Wendy E Kaman
    Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands
    Patient Prefer Adherence 7:559-77. 2013
    ..To determine the current requirements and perceived barriers to the introduction of POC diagnostics in the field of medical microbiology (MM)-POC a prospective online survey (TEMPOtest-QC) was established...
  19. pmc Highly specific protease-based approach for detection of porphyromonas gingivalis in diagnosis of periodontitis
    Wendy E Kaman
    Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands
    J Clin Microbiol 50:104-12. 2012
    ..We envisage that these substrates may improve the specificity of the current enzyme-based diagnosis of periodontitis associated with P. gingivalis...
  20. ncbi request reprint Unveiling electrotransformation of Moraxella catarrhalis as a process of natural transformation
    Patricia Stutzmann Meier
    Institute for Infectious Diseases, University of Bern, Bern, Switzerland
    FEMS Microbiol Lett 262:72-6. 2006
    ..Nontransformability of M. catarrhalis by electrotransformation was observed using double- as well as single-stranded DNA. In addition, the study showed that natural competence is a very constant feature of M. catarrhalis...