Niels Galjart

Summary

Affiliation: Erasmus University
Country: The Netherlands

Publications

  1. doi request reprint Plus-end-tracking proteins and their interactions at microtubule ends
    Niels Galjart
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Curr Biol 20:R528-37. 2010
  2. ncbi request reprint A plus-end raft to control microtubule dynamics and function
    Niels Galjart
    Department of Cell Biology and Genetics, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Opin Cell Biol 15:48-53. 2003
  3. ncbi request reprint Role of CLASP2 in microtubule stabilization and the regulation of persistent motility
    Ksenija Drabek
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Biol 16:2259-64. 2006
  4. pmc Dynamic behavior of GFP-CLIP-170 reveals fast protein turnover on microtubule plus ends
    Katharina A Dragestein
    Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, Netherlands
    J Cell Biol 180:729-37. 2008
  5. doi request reprint Mammalian Navigators are microtubule plus-end tracking proteins that can reorganize the cytoskeleton to induce neurite-like extensions
    Jeffrey van Haren
    Department of Cell Biology and Genetics, Erasmus MC, 3000 CA Rotterdam, The Netherlands
    Cell Motil Cytoskeleton 66:824-38. 2009
  6. doi request reprint History-dependent catastrophes regulate axonal microtubule behavior
    Tatiana Stepanova
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Curr Biol 20:1023-8. 2010
  7. ncbi request reprint CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta
    Gideon Lansbergen
    MGC Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Dev Cell 11:21-32. 2006
  8. doi request reprint The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem cell maintenance
    Ksenija Drabek
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Cell Rep 2:781-8. 2012
  9. pmc CTCF regulates the local epigenetic state of ribosomal DNA repeats
    Suzanne van de Nobelen
    Department of Cell Biology and Genetics, Erasmus MC, The Netherlands
    Epigenetics Chromatin 3:19. 2010
  10. pmc CTCF regulates cell cycle progression of alphabeta T cells in the thymus
    Helen Heath
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 27:2839-50. 2008

Collaborators

Detail Information

Publications32

  1. doi request reprint Plus-end-tracking proteins and their interactions at microtubule ends
    Niels Galjart
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Curr Biol 20:R528-37. 2010
    ....
  2. ncbi request reprint A plus-end raft to control microtubule dynamics and function
    Niels Galjart
    Department of Cell Biology and Genetics, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Opin Cell Biol 15:48-53. 2003
    ..This may, in turn, control the dynamic behaviour of this cytoskeletal network and its anchoring to other structures...
  3. ncbi request reprint Role of CLASP2 in microtubule stabilization and the regulation of persistent motility
    Ksenija Drabek
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Biol 16:2259-64. 2006
    ..Our results suggest that the regional immobilization of CLASP2 allows MT stabilization and promotes directionally persistent motility in fibroblasts...
  4. pmc Dynamic behavior of GFP-CLIP-170 reveals fast protein turnover on microtubule plus ends
    Katharina A Dragestein
    Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, Netherlands
    J Cell Biol 180:729-37. 2008
    ..We propose that the observed loss of fluorescent +TIPs at plus ends does not reflect the behavior of single molecules but is a result of overall structural changes of the MT end...
  5. doi request reprint Mammalian Navigators are microtubule plus-end tracking proteins that can reorganize the cytoskeleton to induce neurite-like extensions
    Jeffrey van Haren
    Department of Cell Biology and Genetics, Erasmus MC, 3000 CA Rotterdam, The Netherlands
    Cell Motil Cytoskeleton 66:824-38. 2009
    ..Combined our data suggest that Navigators are +TIPs that can reorganize the cytoskeleton to guide cell shape changes. Our data are consistent with a role for Navigators in neurite outgrowth...
  6. doi request reprint History-dependent catastrophes regulate axonal microtubule behavior
    Tatiana Stepanova
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Curr Biol 20:1023-8. 2010
    ....
  7. ncbi request reprint CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta
    Gideon Lansbergen
    MGC Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Dev Cell 11:21-32. 2006
    ..We propose that LL5beta and ELKS can form a PIP3-regulated cortical platform to which CLASPs attach distal microtubule ends...
  8. doi request reprint The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem cell maintenance
    Ksenija Drabek
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Cell Rep 2:781-8. 2012
    ..We propose that the CLASP2-mediated stabilization of microtubules is required for proper attachment, homing, and maintenance of hematopoietic stem cells and that this is necessary to sustain c-Mpl transcription...
  9. pmc CTCF regulates the local epigenetic state of ribosomal DNA repeats
    Suzanne van de Nobelen
    Department of Cell Biology and Genetics, Erasmus MC, The Netherlands
    Epigenetics Chromatin 3:19. 2010
    ..To further explore the role of this essential factor, we used a mass spectrometry-based approach to screen for novel CTCF-interacting partners...
  10. pmc CTCF regulates cell cycle progression of alphabeta T cells in the thymus
    Helen Heath
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 27:2839-50. 2008
    ..We propose that CTCF positively regulates cell growth in rapidly dividing thymocytes so that appropriate number of cells are generated before positive and negative selection in the thymus...
  11. pmc CTCF mediates long-range chromatin looping and local histone modification in the beta-globin locus
    Erik Splinter
    Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Genes Dev 20:2349-54. 2006
    ..We postulate that throughout the genome, relative position and stability of CTCF-mediated loops determine their effect on enhancer-promoter interactions, with gene insulation as one possible outcome...
  12. pmc The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis
    Anna Akhmanova
    Department of Cell Biology and Genetics, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    Genes Dev 19:2501-15. 2005
    ..We propose that CLIP-170 has a structural function in the male germline, in particular in spermatid differentiation and sperm head shaping...
  13. ncbi request reprint Differential effects of matrix and growth factors on endothelial and fibroblast motility: application of a modified cell migration assay
    Remco van Horssen
    Laboratory of Experimental Surgical Oncology, Erasmus MC Daniel den Hoed Cancer Centre, 3000 DR Rotterdam, The Netherlands
    J Cell Biochem 99:1536-52. 2006
    ..J. Cell. Biochem. 99: 1536-1552, 2006. (c) 2006 Wiley-Liss, Inc...
  14. ncbi request reprint CLIPs and CLASPs and cellular dynamics
    Niels Galjart
    Department of Cell Biology and Genetics, Erasmus Medical Centre, P O Box 1738, 3000 DR, Rotterdam, The Netherlands
    Nat Rev Mol Cell Biol 6:487-98. 2005
    ..CLIPs and CLASPs interact and cooperate to direct the microtubule network, thereby regulating cellular asymmetry...
  15. pmc Bicaudal D induces selective dynein-mediated microtubule minus end-directed transport
    Casper C Hoogenraad
    MGC Department of Neuroscience, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    EMBO J 22:6004-15. 2003
    ..Our findings provide a novel model system for dissection of the molecular mechanism of dynein motility...
  16. ncbi request reprint LIMK1 and CLIP-115: linking cytoskeletal defects to Williams syndrome
    Casper C Hoogenraad
    Department of Neuroscience, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Bioessays 26:141-50. 2004
    ....
  17. ncbi request reprint Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
    Tatiana Stepanova
    Medical Genetics Center Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands
    J Neurosci 23:2655-64. 2003
    ..We propose that similar mechanisms underlie the specific association of CLIPs and EB1-related proteins with the ends of growing microtubules in non-neuronal and neuronal cells...
  18. pmc LIS1, CLIP-170's key to the dynein/dynactin pathway
    Frédéric M Coquelle
    Institut Curie, Section de Recherche, CNRS UMR 146, Centre Universitaire d Orsay, 91405 Orsay Cedex, France
    Mol Cell Biol 22:3089-102. 2002
    ..This work suggests that LIS1 is a regulated adapter between CLIP-170 and cytoplasmic dynein at sites involved in cargo-MT loading, and/or in the control of MT dynamics...
  19. pmc Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization
    Gideon Lansbergen
    MGC Dept of Cell Biology and of Genetics, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, Netherlands
    J Cell Biol 166:1003-14. 2004
    ..This interaction interferes with the binding of the CLIP-170 to MTs. We propose that conformational changes in CLIP-170 are important for binding to dynactin, LIS1, and the MT tips...
  20. pmc Cytoplasmic linker proteins promote microtubule rescue in vivo
    Yulia A Komarova
    Department of Cell and Molecular Biology, N University Medical School, Chicago, IL 60611, USA
    J Cell Biol 159:589-99. 2002
    ..By promoting a high rescue frequency, CLIPs provide a mechanism by which MT plus ends may be concentrated near the cell margin...
  21. pmc CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex
    Yuko Mimori-Kiyosue
    KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
    J Cell Biol 168:141-53. 2005
    ..We propose that CLASPs can mediate interactions between MT plus ends and the cell cortex and act as local rescue factors, possibly through forming a complex with EB1 at MT tips...
  22. pmc Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytes
    Sunita R Patel
    Division of Hematology, Brigham and Women s Hospital, Dana Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Blood 106:4076-85. 2005
    ..We conclude that while continuous polymerization of microtubules is necessary to support the enlarging proplatelet mass, the sliding of overlapping microtubules is a vital component of proplatelet elongation...
  23. pmc EB1 and EB3 control CLIP dissociation from the ends of growing microtubules
    Yulia Komarova
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611, USA
    Mol Biol Cell 16:5334-45. 2005
    ....
  24. doi request reprint Particle filtering for multiple object tracking in dynamic fluorescence microscopy images: application to microtubule growth analysis
    Ihor Smal
    Department of Medical Informatics, Erasmus MC University Medical Center Rotterdam, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    IEEE Trans Med Imaging 27:789-804. 2008
    ..These demonstrate that the method yields results that are in good agreement with manual tracking performed by expert cell biologists. Our findings suggest that the method may replace laborious manual procedures...
  25. doi request reprint Plasma membrane recruitment of dephosphorylated beta-catenin upon activation of the Wnt pathway
    Jolita Hendriksen
    Department of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    J Cell Sci 121:1793-802. 2008
    ..Our data reveal a new step in the processing of the Wnt signal and suggest regulation of signaling output beyond the level of protein accumulation...
  26. pmc CLIP-170 facilitates the formation of kinetochore-microtubule attachments
    Marvin E Tanenbaum
    Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam, The Netherlands
    EMBO J 25:45-57. 2006
    ..Taken together, our results indicate that CLIP-170 facilitates the formation of kinetochore-microtubule attachments, possibly through direct capture of microtubules at the kinetochore...
  27. pmc Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubules
    Sunita Patel-Hett
    Translational Medicine Division, Brigham and Women s Hospital, Boston, MA 02115, USA
    Blood 111:4605-16. 2008
    ..Thus, the dynamic microtubules associated with the marginal band likely function during both resting and activated platelet states...
  28. ncbi request reprint Cryo electron tomography of vitrified fibroblasts: microtubule plus ends in situ
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    J Struct Biol 161:459-68. 2008
    ..This suggests that frayed ends might be stabilized in the cell cortex...
  29. ncbi request reprint Rao-Blackwellized marginal particle filtering for multiple object tracking in molecular bioimaging
    Ihor Smal
    Departments of Radiology and Medical Informatics, Erasmus MC University Medical Center Rotterdam, The Netherlands
    Inf Process Med Imaging 20:110-21. 2007
    ....
  30. pmc Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network
    Andrey Efimov
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Dev Cell 12:917-30. 2007
    ..We propose that Golgi-emanating microtubules contribute to the asymmetric microtubule networks in polarized cells and support diverse processes including post-Golgi transport to the cell front...
  31. ncbi request reprint Microtubule-binding proteins CLASP1 and CLASP2 interact with actin filaments
    Andrey S Tsvetkov
    Department of Physiology and Biophysics M C 901, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    Cell Motil Cytoskeleton 64:519-30. 2007
    ..We propose that CLASPs facilitate recognition of actin filaments by the plus ends of growing microtubules at the initial stages of actin-microtubule interaction. Cell Motil...
  32. pmc Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends
    Leticia Peris
    Laboratoire du Cytosquelette, Institut National de la Santé et de la Recherche Médicale U366, Département Réponse et Dynamique Cellulaire, Commisariat a l Energie Atomique Grenoble, 38054 Grenoble, France
    J Cell Biol 174:839-49. 2006
    ....