Ron A M Fouchier

Summary

Affiliation: Erasmus MC
Country: The Netherlands

Publications

  1. ncbi request reprint Public health and biosecurity. Restricted data on influenza H5N1 virus transmission
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Science 335:662-3. 2012
  2. pmc Swine influenza virus infection dynamics in two pig farms; results of a longitudinal assessment
    Meritxell Simon-Grifé
    Centre de Recerca en Sanitat Animal CReSA, UAB IRTA, Campus de la Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Vet Res 43:24. 2012
  3. ncbi request reprint Newer respiratory virus infections: human metapneumovirus, avian influenza virus, and human coronaviruses
    Ron Am Fouchier
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Curr Opin Infect Dis 18:141-6. 2005
  4. pmc Laboratory tests for SARS: powerful or peripheral?
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    CMAJ 170:63-4. 2004
  5. ncbi request reprint Epidemiology of low pathogenic avian influenza viruses in wild birds
    R A M Fouchier
    National Influenza Centre and Department of Virology, Erasmus Medical Centre, Rotterdam, The Netherlands
    Rev Sci Tech 28:49-58. 2009
  6. ncbi request reprint Use of antigenic cartography in vaccine seed strain selection
    Ron A M Fouchier
    Department of Virology, Erasmus MC, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Avian Dis 54:220-3. 2010
  7. pmc Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls
    Ron A M Fouchier
    Dept of Virology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Virol 79:2814-22. 2005
  8. pmc A previously undescribed coronavirus associated with respiratory disease in humans
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Center, and CoroNovative B V, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 101:6212-6. 2004
  9. ncbi request reprint Animal influenza virus surveillance
    Ron A M Fouchier
    Department of Virology, Erasmus MC Rotterdam, Dr Molewaterplein 50, 3015 GE, Rotterdam, The Netherlands
    Vaccine 21:1754-7. 2003
  10. pmc Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome
    Ron A M Fouchier
    Department of Virology and National Influenza Center, Erasmus Medical Center, Dr Molewaterplein 50, 3015 GE, Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 101:1356-61. 2004

Detail Information

Publications101 found, 100 shown here

  1. ncbi request reprint Public health and biosecurity. Restricted data on influenza H5N1 virus transmission
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Science 335:662-3. 2012
  2. pmc Swine influenza virus infection dynamics in two pig farms; results of a longitudinal assessment
    Meritxell Simon-Grifé
    Centre de Recerca en Sanitat Animal CReSA, UAB IRTA, Campus de la Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Vet Res 43:24. 2012
    ..These findings led us to propose that influenza virus infection in pigs might present different patterns, from an epidemic outbreak to an endemic form with different waves of infections with a lower incidence...
  3. ncbi request reprint Newer respiratory virus infections: human metapneumovirus, avian influenza virus, and human coronaviruses
    Ron Am Fouchier
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Curr Opin Infect Dis 18:141-6. 2005
    ..Here we review the recent literature on these respiratory viruses...
  4. pmc Laboratory tests for SARS: powerful or peripheral?
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    CMAJ 170:63-4. 2004
  5. ncbi request reprint Epidemiology of low pathogenic avian influenza viruses in wild birds
    R A M Fouchier
    National Influenza Centre and Department of Virology, Erasmus Medical Centre, Rotterdam, The Netherlands
    Rev Sci Tech 28:49-58. 2009
    ..Here, the authors review the current knowledge of the (molecular) epidemiology, genetics and evolution of LPAI viruses in wild birds, and identify some important gaps in current knowledge...
  6. ncbi request reprint Use of antigenic cartography in vaccine seed strain selection
    Ron A M Fouchier
    Department of Virology, Erasmus MC, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Avian Dis 54:220-3. 2010
    ..Considerable antigenic variation was also observed within and between H5N1 clades. These observations have important implications for the efficacy and long-term use of poultry vaccines...
  7. pmc Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls
    Ron A M Fouchier
    Dept of Virology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Virol 79:2814-22. 2005
    ..We propose that sequence analyses of HA and NA genes of influenza A viruses be used for the rapid identification of existing and novel HA and NA subtypes...
  8. pmc A previously undescribed coronavirus associated with respiratory disease in humans
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Center, and CoroNovative B V, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 101:6212-6. 2004
    ..Moreover, it will be important to discriminate this previously undescribed coronavirus from HCoV 229E and OC43 and the severe acute respiratory syndrome coronavirus...
  9. ncbi request reprint Animal influenza virus surveillance
    Ron A M Fouchier
    Department of Virology, Erasmus MC Rotterdam, Dr Molewaterplein 50, 3015 GE, Rotterdam, The Netherlands
    Vaccine 21:1754-7. 2003
    ..Here we provide a brief overview of some of the data from the past and give our view on how the continued and intensified animal influenza virus surveillance may aid in the early recognition and management of outbreaks in the future...
  10. pmc Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome
    Ron A M Fouchier
    Department of Virology and National Influenza Center, Erasmus Medical Center, Dr Molewaterplein 50, 3015 GE, Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 101:1356-61. 2004
    ..Because H7N7 viruses have caused disease in mammals, including horses, seals, and humans, on several occasions in the past, they may be unusual in their zoonotic potential and, thus, form a pandemic threat to humans...
  11. doi request reprint Airborne transmission of influenza A/H5N1 virus between ferrets
    Sander Herfst
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Science 336:1534-41. 2012
    ..Thus, avian A/H5N1 influenza viruses can acquire the capacity for airborne transmission between mammals without recombination in an intermediate host and therefore constitute a risk for human pandemic influenza...
  12. pmc Insertion of a multibasic cleavage motif into the hemagglutinin of a low-pathogenic avian influenza H6N1 virus induces a highly pathogenic phenotype
    Vincent J Munster
    Department of Virology, Erasmus Medical Center, PO Box 2040, Rotterdam, Netherlands
    J Virol 84:7953-60. 2010
    ....
  13. pmc Efficacy of vaccination with different combinations of MF59-adjuvanted and nonadjuvanted seasonal and pandemic influenza vaccines against pandemic H1N1 (2009) influenza virus infection in ferrets
    Judith M A van den Brand
    Department of Virology, Erasmus Medical Center, P O Box 2040, 3000 CA Rotterdam, Netherlands
    J Virol 85:2851-8. 2011
    ....
  14. doi request reprint Generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters
    Sander Herfst
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Gen Virol 89:1553-62. 2008
    ..Further mapping of the molecular determinants of attenuation of HMPV should be the subject of future studies...
  15. doi request reprint Immunogenicity and efficacy of two candidate human metapneumovirus vaccines in cynomolgus macaques
    Sander Herfst
    Department of Virology, Erasmus MC, Rotterdam, The Netherlands
    Vaccine 26:4224-30. 2008
    ..Both vaccines induced HMPV-specific immune responses, but the rapid waning of immunity is a challenging obstacle for vaccine development...
  16. pmc Mallards and highly pathogenic avian influenza ancestral viruses, northern Europe
    Vincent J Munster
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Emerg Infect Dis 11:1545-51. 2005
    ....
  17. pmc Protection of mice against lethal infection with highly pathogenic H7N7 influenza A virus by using a recombinant low-pathogenicity vaccine strain
    Emmie de Wit
    Department of Virology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Virol 79:12401-7. 2005
    ..The data presented suggest that vaccine candidates based on low-pathogenicity avian influenza A viruses, which can be prepared ahead of pandemic threats, can be efficacious if an effective adjuvant is used...
  18. pmc In vitro assessment of attachment pattern and replication efficiency of H5N1 influenza A viruses with altered receptor specificity
    Salin Chutinimitkul
    Department of Virology and National Influenza Center, Erasmus MC, P O Box 2040, 3000CA Rotterdam, Netherlands
    J Virol 84:6825-33. 2010
    ....
  19. doi request reprint Evaluation of a modified vaccinia virus Ankara (MVA)-based candidate pandemic influenza A/H1N1 vaccine in the ferret model
    Joost H C M Kreijtz
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Gen Virol 91:2745-52. 2010
    ..In contrast, two immunizations induced robust antibody responses and protected ferrets from developing severe disease, confirming that MVA is an attractive influenza vaccine production platform...
  20. pmc Limited airborne transmission of H7N9 influenza A virus between ferrets
    Mathilde Richard
    Department of Viroscience, Erasmus Medical Center, 3015GE Rotterdam, The Netherlands
    Nature 501:560-3. 2013
    ..This A(H7N9) virus outbreak highlights the need for increased understanding of the determinants of efficient airborne transmission of avian influenza viruses between mammals. ..
  21. doi request reprint Specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses
    Miranda de Graaf
    Department of Virology, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    J Gen Virol 89:975-83. 2008
    ....
  22. doi request reprint Pathogenesis and transmission of swine-origin 2009 A(H1N1) influenza virus in ferrets
    Vincent J Munster
    National Influenza Center and Department of Virology, Erasmus Medical Center, 3015GE Rotterdam, The Netherlands
    Science 325:481-3. 2009
    ..These data suggest that the 2009 A(H1N1) influenza virus has the ability to persist in the human population, potentially with more severe clinical consequences...
  23. ncbi request reprint H5N1 Virus Attachment to Lower Respiratory Tract
    Debby van Riel
    Department of Virology, Erasmus Medical Center, 3015 GE Rotterdam, Netherlands
    Science 312:399. 2006
    ..These findings may explain, at least in part, the localization and severity of H5N1 viral pneumonia in humans. They also identify the cat and the ferret as suitable experimental animals based on this criterion...
  24. pmc Wild ducks as long-distance vectors of highly pathogenic avian influenza virus (H5N1)
    Juthatip Keawcharoen
    Erasmus Medical Center, Rotterdam, The Netherlands
    Emerg Infect Dis 14:600-7. 2008
    ....
  25. pmc Spatial, temporal, and species variation in prevalence of influenza A viruses in wild migratory birds
    Vincent J Munster
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS Pathog 3:e61. 2007
    ..These data are useful for designing new surveillance programs and are particularly relevant due to increased interest in avian influenza in wild birds...
  26. pmc Vaccination against human influenza A/H3N2 virus prevents the induction of heterosubtypic immunity against lethal infection with avian influenza A/H5N1 virus
    Rogier Bodewes
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS ONE 4:e5538. 2009
    ..These findings may have implications for the general recommendation to vaccinate all healthy children against seasonal influenza in the light of the current pandemic threat caused by highly pathogenic avian A/H5N1 influenza viruses...
  27. pmc MVA-based H5N1 vaccine affords cross-clade protection in mice against influenza A/H5N1 viruses at low doses and after single immunization
    Joost H C M Kreijtz
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS ONE 4:e7790. 2009
    ..It was concluded that MVA-based vaccines allowed significant dose-sparing and afford cross-clade protection, also after a single immunization, which are favorable properties for an H5N1 vaccine candidate...
  28. doi request reprint Vaccination with whole inactivated virus vaccine affects the induction of heterosubtypic immunity against influenza virus A/H5N1 and immunodominance of virus-specific CD8+ T-cell responses in mice
    Rogier Bodewes
    Department of Virology, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    J Gen Virol 91:1743-53. 2010
    ..These findings may have implications for vaccination strategies aimed at the induction of protective immunity to seasonal and/or pandemic influenza...
  29. pmc Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments
    Eefje J A Schrauwen
    Viroscience Lab, Postgraduate School Molecular Medicine, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS ONE 8:e59889. 2013
    ..The neuraminidase and matrix gene segments of human influenza viruses showed the highest genetic compatibility with HPAI H5N1 virus...
  30. pmc Human and avian influenza viruses target different cells in the lower respiratory tract of humans and other mammals
    Debby van Riel
    Department of Virology, Erasmus MC, P O Box 2040, 3015 CA, Rotterdam, The Netherlands
    Am J Pathol 171:1215-23. 2007
    ..The PVA in human respiratory tract most closely mirrored that in ferrets and pigs for human influenza viruses and that in ferrets, pigs, and cats for avian influenza viruses...
  31. ncbi request reprint Immunization of Syrian golden hamsters with F subunit vaccine of human metapneumovirus induces protection against challenge with homologous or heterologous strains
    Sander Herfst
    Department of Virology, Erasmus MC, Rotterdam, The Netherlands
    J Gen Virol 88:2702-9. 2007
    ..In conclusion, a soluble F subunit vaccine for hMPV that induces cross-protective immunity for infection of the lower respiratory tract in Syrian golden hamsters has been generated...
  32. ncbi request reprint The molecular basis of the pathogenicity of the Dutch highly pathogenic human influenza A H7N7 viruses
    Vincent J Munster
    Department of Virology and National Influenza Center, Erasmus Medical Center, Rotterdam, The Netherlands
    J Infect Dis 196:258-65. 2007
    ..We conclude that the influenza virus HA and PB2 genes should be the prime targets for molecular surveillance during outbreaks of zoonotic HPAI viruses...
  33. ncbi request reprint Virology: SARS virus infection of cats and ferrets
    Byron E E Martina
    Institute of Virology, Erasmus Medical Centre, 3015 GE Rotterdam, The Netherlands
    Nature 425:915. 2003
  34. pmc Possible increased pathogenicity of pandemic (H1N1) 2009 influenza virus upon reassortment
    Eefje J A Schrauwen
    National Influenza Centre and Erasmus Medical Center Department of Virology, Rotterdam, The Netherlands
    Emerg Infect Dis 17:200-8. 2011
    ....
  35. pmc Virulence-associated substitution D222G in the hemagglutinin of 2009 pandemic influenza A(H1N1) virus affects receptor binding
    Salin Chutinimitkul
    Department of Virology, Erasmus Medical Center Rotterdam, Rotterdam, Netherlands
    J Virol 84:11802-13. 2010
    ..Altered receptor specificity of the virus with D222G thus affected interaction with cells of the human lower respiratory tract, possibly explaining the observed association with enhanced disease in humans...
  36. pmc Experimental human metapneumovirus infection of cynomolgus macaques (Macaca fascicularis) results in virus replication in ciliated epithelial cells and pneumocytes with associated lesions throughout the respiratory tract
    Thijs Kuiken
    Departments of Virology and Immunology, Erasmus Medical Center, Rotterdam, The Netherlands
    Am J Pathol 164:1893-900. 2004
    ..This study demonstrates that hMPV is a respiratory pathogen and indicates that viral replication is short-lived, polarized to the apical surface, and occurs primarily in ciliated respiratory epithelial cells...
  37. ncbi request reprint Pegylated interferon-alpha protects type 1 pneumocytes against SARS coronavirus infection in macaques
    Bart L Haagmans
    Department of Virology, Erasmus Medical Centre, PO Box 1738, 3000 DR, Rotterdam, Netherlands
    Nat Med 10:290-3. 2004
    ..Postexposure treatment with pegylated IFN-alpha yielded intermediate results. We therefore suggest that pegylated IFN-alpha protects type 1 pneumocytes from SCV infection, and should be considered a candidate drug for SARS therapy...
  38. pmc Binding of DC-SIGN to the hemagglutinin of influenza A viruses supports virus replication in DC-SIGN expressing cells
    Marine L B Hillaire
    Erasmus Medical Centre, Rotterdam, The Netherlands
    PLoS ONE 8:e56164. 2013
    ..Furthermore, the efficiency of DC-SIGN mediated infection is dependent on the extent of glycosylation of the viral hemagglutinin...
  39. pmc Infection of the upper respiratory tract with seasonal influenza A(H3N2) virus induces protective immunity in ferrets against infection with A(H1N1)pdm09 virus after intranasal, but not intratracheal, inoculation
    Rogier Bodewes
    Viroscience Lab, Erasmus MC, Rotterdam, Netherlands
    J Virol 87:4293-301. 2013
    ..These findings are of interest in light of the development of so-called universal influenza vaccines that aim at the induction of cross-reactive T cell responses...
  40. pmc The multibasic cleavage site in H5N1 virus is critical for systemic spread along the olfactory and hematogenous routes in ferrets
    Eefje J A Schrauwen
    National Influenza Center and Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Virol 86:3975-84. 2012
    ..In conclusion, HPAI H5N1 virus can spread systemically via two different routes, olfactory and hematogenous, in ferrets. This systemic spread was dependent on the presence of the MBCS in HA...
  41. ncbi request reprint Experimental infection of macaques with human metapneumovirus induces transient protective immunity
    Bernadette G van den Hoogen
    Department of Virology, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    J Gen Virol 88:1251-9. 2007
    ..Eight months later, protection had waned to virtually undetectable levels. This study demonstrates that experimental hMPV infection induces transient protective immunity...
  42. doi request reprint Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus
    Judith M A van den Brand
    Department of Virology, Erasmus Medical Centre, Rotterdam, The Netherlands
    J Infect Dis 201:993-9. 2010
    ..Three influenza pandemics of the previous century caused variable mortality, which largely depended on the development of severe pneumonia. However, the ability of the new H1N1 virus to cause pneumonia is poorly understood...
  43. ncbi request reprint Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome
    Thijs Kuiken
    Department of Virology, Erasmus Medical Centre, PO Box 1738, 3000 DR, Rotterdam, Netherlands
    Lancet 362:263-70. 2003
    ..We did clinical and experimental studies to assess the role of this virus in the cause of SARS...
  44. pmc Assessment of the antiviral properties of recombinant porcine SP-D against various influenza A viruses in vitro
    Marine L B Hillaire
    Erasmus Medical Centre, Rotterdam, The Netherlands
    PLoS ONE 6:e25005. 2011
    ..It was concluded that recombinant porcine SP-D holds promise as a novel antiviral agent against influenza and further development and evaluation in vivo seems warranted...
  45. pmc Pathogenesis of Influenza A/H5N1 virus infection in ferrets differs between intranasal and intratracheal routes of inoculation
    Rogier Bodewes
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Am J Pathol 179:30-6. 2011
    ..The method of virus inoculation requires careful consideration in the design of ferret experiments as a model for influenza A/H5N1 in humans...
  46. ncbi request reprint A reverse-genetics system for Influenza A virus using T7 RNA polymerase
    Emmie de Wit
    National Influenza Center, Department of Virology and Postgraduate School of Molecular Medicine, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    J Gen Virol 88:1281-7. 2007
    ..Thus, a reverse-genetics system for the rescue of Influenza A virus has been developed, which will be useful for fundamental research and vaccine seed strain production in a variety of cell lines...
  47. pmc Molecular determinants of adaptation of highly pathogenic avian influenza H7N7 viruses to efficient replication in the human host
    Emmie de Wit
    National Influenza Center and Department of Virology, Erasmus Medical Center, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    J Virol 84:1597-606. 2010
    ....
  48. pmc Insertion of a multibasic cleavage site in the haemagglutinin of human influenza H3N2 virus does not increase pathogenicity in ferrets
    Eefje J A Schrauwen
    National Influenza Center and Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Gen Virol 92:1410-5. 2011
    ..This study indicates that acquisition of an MBCS alone is insufficient to increase pathogenicity of a prototypical seasonal human H3N2 virus...
  49. ncbi request reprint Attachment of infectious influenza A viruses of various subtypes to live mammalian and avian cells as measured by flow cytometry
    Guus F Rimmelzwaan
    Department of Virology, Postgraduate School Molecular Medicine, Erasmus Medical Center, Rotterdam, The Netherlands
    Virus Res 129:175-81. 2007
    ..Virus attachment was not always predictive for efficient infection of the cells...
  50. ncbi request reprint Recombinant modified vaccinia virus Ankara-based vaccine induces protective immunity in mice against infection with influenza virus H5N1
    Joost H C M Kreijtz
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Infect Dis 195:1598-606. 2007
    ..Our data suggest that recombinant MVA expressing the HA of influenza virus A/Vietnam/1194/04 is a promising alternative vaccine candidate that could be used for the induction of protective immunity against various H5N1 influenza strains...
  51. doi request reprint Use of GFP-expressing influenza viruses for the detection of influenza virus A/H5N1 neutralizing antibodies
    Guus F Rimmelzwaan
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Vaccine 29:3424-30. 2011
    ....
  52. ncbi request reprint Preferential HLA usage in the influenza virus-specific CTL response
    Adrianus C M Boon
    Department of Virology and World Health Organization National Influenza Center, Erasmus Medical Center, Rotterdam, The Netherlands
    J Immunol 172:4435-43. 2004
    ..In contrast, the CTL response to influenza B virus was mainly directed toward HLA-B*0801-restricted epitopes. Thus, the preferential use of HLA alleles depended on the virus studied...
  53. doi request reprint Cross-protective immunity against influenza pH1N1 2009 viruses induced by seasonal influenza A (H3N2) virus is mediated by virus-specific T-cells
    Marine L B Hillaire
    Erasmus Medical Centre, Rotterdam, The Netherlands
    J Gen Virol 92:2339-49. 2011
    ....
  54. doi request reprint Evolutionary dynamics of human and avian metapneumoviruses
    Miranda de Graaf
    Department of Virology, Erasmus Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    J Gen Virol 89:2933-42. 2008
    ..The relative genetic diversity observed in the four HMPV lineages was low, most likely reflecting continual population bottlenecks, with only limited evidence for positive selection...
  55. doi request reprint Fusion protein is the main determinant of metapneumovirus host tropism
    Miranda de Graaf
    Department of Virology, Erasmus Medical Centre, PO Box 2040, 3000 CA Rotterdam, The Netherlands
    J Gen Virol 90:1408-16. 2009
    ..This study provides insight into the determinants of host tropism and membrane fusion of metapneumoviruses...
  56. pmc The hypervariable immunodominant NP418-426 epitope from the influenza A virus nucleoprotein is recognized by cytotoxic T lymphocytes with high functional avidity
    Adrianus C M Boon
    Department of Virology, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    J Virol 80:6024-32. 2006
    ..01). It was speculated that the high functional avidity of NP(418-426)-specific CTL was responsible for the diversification of this influenza A virus CTL epitope...
  57. pmc Seasonal and pandemic human influenza viruses attach better to human upper respiratory tract epithelium than avian influenza viruses
    Debby van Riel
    Erasmus Medical Center, Department of Virology, CA Rotterdam, The Netherlands
    Am J Pathol 176:1614-8. 2010
    ..These results suggest that the ability of an influenza virus to attach to human upper respiratory tract is a critical factor for efficient transmission in the human population...
  58. pmc Practical considerations for high-throughput influenza A virus surveillance studies of wild birds by use of molecular diagnostic tests
    Vincent J Munster
    National Influenza Center and Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Clin Microbiol 47:666-73. 2009
    ..Moreover, the lessons learned from current large-scale influenza A virus surveillance activities could be valuable for other pathogen surveillance programs in the future...
  59. pmc Recovery of human metapneumovirus genetic lineages a and B from cloned cDNA
    Sander Herfst
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Virol 78:8264-70. 2004
    ..The recombinant viruses replicated with kinetics similar to those of the parental viruses in Vero cells. This reverse genetics system provides an important new tool for applied and fundamental research...
  60. doi request reprint Annual influenza vaccination affects the development of heterosubtypic immunity
    Rogier Bodewes
    Department of Virology, Erasmus MC, Rotterdam, The Netherlands
    Vaccine 30:7407-10. 2012
    ..These findings highlight the importance of the development of vaccines that provide protection against influenza A viruses of all subtypes...
  61. doi request reprint Human T-cells directed to seasonal influenza A virus cross-react with 2009 pandemic influenza A (H1N1) and swine-origin triple-reassortant H3N2 influenza viruses
    Marine L B Hillaire
    Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
    J Gen Virol 94:583-92. 2013
    ..It was concluded that cross-reacting T-cells had the potential to afford protective immunity against A(H1N1)pdm09 viruses during the pandemic and offer some degree of protection against infection with A(H3N2)v viruses...
  62. pmc Genetic evolution of the neuraminidase of influenza A (H3N2) viruses from 1968 to 2009 and its correspondence to haemagglutinin evolution
    Kim B Westgeest
    Department of Virology, Erasmus Medical Center, 3000 CA, Rotterdam, The Netherlands
    J Gen Virol 93:1996-2007. 2012
    ..The differences found between the evolution of NA and HA1 warrant further analysis of the evolution of NA at the phenotypic level, as has been done previously for HA...
  63. ncbi request reprint Highly pathogenic avian influenza (H7N7): vaccination of zoo birds and transmission to non-poultry species
    Joost D W Philippa
    Institute of Virology, Erasmus, MC, Dr Molenwaterplein 50, Rotterdam, The Netherlands
    Vaccine 23:5743-50. 2005
    ....
  64. ncbi request reprint Towards improved influenza A virus surveillance in migrating birds
    Vincent J Munster
    Department of Virology, Erasmus Medical Centre, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Vaccine 24:6729-33. 2006
    ..This is particularly relevant if wild bird surveillance studies are used as an "early warning system" for the arrival of the H5N1 HPAI virus in a country or region and to assess the risk posed by these viruses in general...
  65. doi request reprint Influenza virus CTL epitopes, remarkably conserved and remarkably variable
    Guus F Rimmelzwaan
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Vaccine 27:6363-5. 2009
    ..A mutational analysis of this epitope indicated that it is under functional constraints. Also in influenza A viruses of other subtypes, including H5N1, the M1(58-66) is highly conserved...
  66. pmc Host behaviour and physiology underpin individual variation in avian influenza virus infection in migratory Bewick's swans
    Bethany J Hoye
    Department of Animal Ecology, Netherlands Institute of Ecology NIOO KNAW, Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands
    Proc Biol Sci 279:529-34. 2012
    ..Together, these results demonstrate the potential for heterogeneity in infection to have a profound influence on the dynamics of pathogens, with concomitant impacts on host habitat selection and fitness...
  67. pmc Multidrug resistant 2009 A/H1N1 influenza clinical isolate with a neuraminidase I223R mutation retains its virulence and transmissibility in ferrets
    Erhard Van Der Vries
    Erasmus Medical Centre, Department of Virology, Rotterdam, The Netherlands
    PLoS Pathog 7:e1002276. 2011
    ..It is important to be vigilant for this mutation in influenza surveillance and to continue efforts to increase the arsenal of antiviral drugs to combat influenza...
  68. pmc Influenza A virus (H5N1) infection in cats causes systemic disease with potential novel routes of virus spread within and between hosts
    Guus F Rimmelzwaan
    Department of Virology, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Am J Pathol 168:176-83; quiz 364. 2006
    ..This study in cats demonstrates that H5N1 virus infection causes systemic disease and spreads by potentially novel routes within and between mammalian hosts...
  69. ncbi request reprint An amino acid substitution in the influenza A virus hemagglutinin associated with escape from recognition by human virus-specific CD4+ T-cells
    Eufemia G M Berkhoff
    Department of Virology and Postgraduate School Molecular Medicine, Erasmus Medical Center, Rotterdam, The Netherlands
    Virus Res 126:282-7. 2007
    ..It was speculated that influenza viruses could evade recognition by virus-specific CD4+ T-cells, at least temporarily...
  70. pmc A family-wide RT-PCR assay for detection of paramyxoviruses and application to a large-scale surveillance study
    Sander van Boheemen
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    PLoS ONE 7:e34961. 2012
    ..The single round family-wide RT-PCR assay described here is a useful tool for the detection of known and unknown paramyxoviruses, and screening of large sample collections from humans and animals...
  71. pmc Annual vaccination against influenza virus hampers development of virus-specific CD8⁺ T cell immunity in children
    Rogier Bodewes
    Department of Virology, Erasmus Medical Center, Dr Molewaterplein 50, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    J Virol 85:11995-2000. 2011
    ..The consequences of these findings are discussed in the light of the development of protective immunity to seasonal and future pandemic influenza viruses...
  72. ncbi request reprint Prevalence and clinical symptoms of human metapneumovirus infection in hospitalized patients
    Bernadette G van den Hoogen
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Infect Dis 188:1571-7. 2003
    ..Treatment with antibiotics and corticosteroids was reported more frequently in hMPV-infected children. From these data, we conclude that hMPV is an important pathogen associated with RTI...
  73. ncbi request reprint Evidence for specific packaging of the influenza A virus genome from conditionally defective virus particles lacking a polymerase gene
    Emmie de Wit
    National Influenza Center and Department of Virology, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Vaccine 24:6647-50. 2006
    ..We conclude that sequences in the 5' and 3' ends of the polymerase gene segments contain packaging signals. Our methods will facilitate the further mapping of the packaging signals of influenza A virus...
  74. pmc Low-pH-induced membrane fusion mediated by human metapneumovirus F protein is a rare, strain-dependent phenomenon
    Sander Herfst
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Virol 82:8891-5. 2008
    ..Only 6% of all HMPV lineage A F sequences have 294G, and none of the lineage B sequences have 294G. Thus, acidic pH is not a general trigger of HMPV F proteins for activity...
  75. pmc Antigenic and genetic variability of human metapneumoviruses
    Bernadette G van den Hoogen
    Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands
    Emerg Infect Dis 10:658-66. 2004
    ..Each serotype could be divided into two genetic lineages, but these did not reflect major antigenic differences...
  76. ncbi request reprint Rapid sequencing of the non-coding regions of influenza A virus
    Emmie de Wit
    National Influenza Center and Department of Virology, Erasmus MC, Rotterdam, The Netherlands
    J Virol Methods 139:85-9. 2007
    ..These PCR fragments can be sequenced directly without the need for cloning PCR fragments first. This method was used to sequence the NCRs of A/Bilthoven/16190/68 (H3N2) and A/Turkey/Turkey/1/05 (H5N1)...
  77. ncbi request reprint Aetiology: Koch's postulates fulfilled for SARS virus
    Ron A M Fouchier
    Department of Virology, Erasmus Medical Centre, 3015 GE Rotterdam, The Netherlands
    Nature 423:240. 2003
  78. ncbi request reprint Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments
    Emmie de Wit
    National Influenza Center and Department of Virology, Erasmus MC, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Virus Res 103:155-61. 2004
    ..In conclusion, high-titer virus stocks of recombinant influenza A/PR/8/34 virus can be produced as well as virus stocks with much lower titers, but without the requirement of virus amplification through replication...
  79. ncbi request reprint Clinical impact and diagnosis of human metapneumovirus infection
    Bernadette G van den Hoogen
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    Pediatr Infect Dis J 23:S25-32. 2004
    ..To estimate the impact of hMPV infection in a hospital setting and in the community at large, we reviewed these surveys to establish the burden of disease of hMPV infection...
  80. pmc Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages
    Jeroen Maertzdorf
    Vironovative BV, Rotterdam, The Netherlands
    J Clin Microbiol 42:981-6. 2004
    ..Here we describe the development and evaluation of a real-time reverse transcriptase PCR assay that detects human metapneumovirus from the four known genetic lineages with equal specificities and sensitivities...
  81. doi request reprint Influenza virus RNA structure: unique and common features
    Alexander P Gultyaev
    Department of Virology, Erasmus Medical Center, Rotterdam, Netherlands
    Int Rev Immunol 29:533-56. 2010
    ..Various ideas and hypotheses about the roles of influenza virus RNA folding in the virus replication are also discussed in relation to other viruses...
  82. ncbi request reprint Vaccination approaches to combat human metapneumovirus lower respiratory tract infections
    Sander Herfst
    Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Clin Virol 41:49-52. 2008
    ..Here we give an overview of the immunogenicity and protective efficacy of a variety of live attenuated, virus vectored, inactivated virus and subunit vaccines that have been tested in animal models...
  83. ncbi request reprint Recognition of homo- and heterosubtypic variants of influenza A viruses by human CD8+ T lymphocytes
    Adrianus C M Boon
    Department of Virology and WHO National Influenza Center, Erasmus MC, Rotterdam, The Netherlands
    J Immunol 172:2453-60. 2004
    ....
  84. ncbi request reprint Influenza virus-specific cytotoxic T lymphocytes: a correlate of protection and a basis for vaccine development
    Guus F Rimmelzwaan
    Erasmus Medical Center, Department of Virology, Rotterdam, The Netherlands
    Curr Opin Biotechnol 18:529-36. 2007
    ..In the present paper, the possibility to develop (cross-reactive) CTL-inducing vaccines is discussed...
  85. ncbi request reprint Analysis of the genomic sequence of a human metapneumovirus
    Bernadette G van den Hoogen
    Department of Virology, Erasmus Medical Center Rotterdam, Dr Molewaterplein 50, Rotterdam, 3015 GE, The Netherlands
    Virology 295:119-32. 2002
    ....
  86. pmc Emerging influenza
    Emmie de Wit
    Department of Virology and National Influenza Center, Erasmus Medical Center, P O Box 2040, 3000CA Rotterdam, The Netherlands
    J Clin Virol 41:1-6. 2008
    ..Therefore, monitoring these and additional genetic changes upon zoonotic infections is important. Identification of genetic changes responsible for transmission between mammals will be an important task for the near future...
  87. ncbi request reprint Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany
    N Engl J Med 348:1967-76. 2003
    ..The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent...
  88. pmc Surveillance of influenza A virus in migratory waterfowl in northern Europe
    Anders Wallensten
    Smedby Health Center, Kalmar, Sweden
    Emerg Infect Dis 13:404-11. 2007
    ..We argue that wild bird screening can function as a sentinel system, and we give an example of how it could have been used to forecast a remote and deadly outbreak of influenza A in poultry...
  89. pmc Isolation and characterization of monoclonal antibodies which neutralize human metapneumovirus in vitro and in vivo
    Nancy D Ulbrandt
    MedImmune, Inc, 1 MedImmune Way, Gaithersburg, MD 20878, USA
    J Virol 80:7799-806. 2006
    ..The data suggest that a monoclonal antibody could be used prophylactically to prevent lower respiratory tract disease caused by hMPV...
  90. ncbi request reprint An improved plaque reduction virus neutralization assay for human metapneumovirus
    Miranda de Graaf
    J Virol Methods 143:169-74. 2007
    ....
  91. pmc Avian influenza H5N1 in tigers and leopards
    Juthatip Keawcharoen
    Chulalongkorn University, Bangkok, Thailand
    Emerg Infect Dis 10:2189-91. 2004
    ..This finding extends the host range of influenza virus and has implications for influenza virus epidemiology and wildlife conservation...
  92. ncbi request reprint Mapping the antigenic and genetic evolution of influenza virus
    Derek J Smith
    Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK
    Science 305:371-6. 2004
    ..Further, this approach offers a route to predicting the relative success of emerging strains, which could be achieved by quantifying the combined effects of population level immune escape and viral fitness on strain evolution...
  93. doi request reprint The global circulation of seasonal influenza A (H3N2) viruses
    Colin A Russell
    Department of Zoology, University of Cambridge, Cambridge, UK
    Science 320:340-6. 2008
    ....
  94. ncbi request reprint Global patterns of influenza a virus in wild birds
    Bjorn Olsen
    Department of Infectious Diseases, Umea University, SE 90187 Umea, Sweden
    Science 312:384-8. 2006
    ....
  95. pmc Genetic characterization of HPAI (H5N1) viruses from poultry and wild vultures, Burkina Faso
    Mariette F Ducatez
    National Public Health Laboratory, Luxembourg
    Emerg Infect Dis 13:611-3. 2007
    ..Hooded vultures could potentially be vectors or sentinels of influenza subtype H5N1, as are cats and swans elsewhere...
  96. doi request reprint The challenges of eliciting neutralizing antibodies to HIV-1 and to influenza virus
    Gunilla B Karlsson Hedestam
    Department of Microbiology, Tumour and Cell Biology, Karolinska Institutet, 171 77 Stockholm, Sweden
    Nat Rev Microbiol 6:143-55. 2008
    ..Efforts to develop broadly neutralizing vaccines against either HIV-1 or influenza virus might establish a paradigm for future vaccines against highly variable pathogens...
  97. pmc Effects of human metapneumovirus and respiratory syncytial virus antigen insertion in two 3' proximal genome positions of bovine/human parainfluenza virus type 3 on virus replication and immunogenicity
    Roderick S Tang
    MedImmune Vaccines, Inc, Mountain View, California 94043, USA
    J Virol 77:10819-28. 2003
    ....
  98. ncbi request reprint Identification of small-animal and primate models for evaluation of vaccine candidates for human metapneumovirus (hMPV) and implications for hMPV vaccine design
    Mia MacPhail
    MedImmune Vaccines Inc, 297 North Bernardo Avenue, Mountain View, CA 94043, USA
    J Gen Virol 85:1655-63. 2004
    ..Although these animal models do not mimic human hMPV disease signs, they will nevertheless be invaluable for the future evaluation of hMPV antivirals, mAbs and vaccines...
  99. ncbi request reprint A host-range restricted parainfluenza virus type 3 (PIV3) expressing the human metapneumovirus (hMPV) fusion protein elicits protective immunity in African green monkeys
    Roderick S Tang
    MedImmune Vaccines Inc, 297 North Bernardo Avenue, Mountain View, CA 94043, USA
    Vaccine 23:1657-67. 2005
    ..These studies showed that b/h PIV3/hMPV F2 was immunogenic, protective and attenuated in non-human primates and warrants further evaluation in humans as a vaccine candidate for prevention of hMPV-associated respiratory tract diseases...
  100. ncbi request reprint High prevalence of influenza A virus in ducks caught during spring migration through Sweden
    Anders Wallensten
    Smedby Health Center, Kalmar County Council, SE 39471 Kalmar, Sweden
    Vaccine 24:6734-5. 2006
    ..In contrast to what has been found in North American studies; Eurasian spring migrating ducks passing through Sweden had a relatively high prevalence of influenza A virus...