Lennard J M Dekker

Summary

Affiliation: Erasmus MC
Country: The Netherlands

Publications

  1. ncbi Multiple sclerosis-related proteins identified in cerebrospinal fluid by advanced mass spectrometry
    Marcel P Stoop
    Laboratories of Neuro Oncology Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Centre, Rotterdam, The Netherlands
    Proteomics 8:1576-85. 2008
  2. ncbi Label-free peptide profiling of Orbitrap™ full mass spectra
    Mark K Titulaer
    Laboratory of Neuro Oncology and Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Center, Dr, Molewaterplein 50, P, O, Box 2040, 3000 CA Rotterdam, The Netherlands
    BMC Res Notes 4:21. 2011
  3. ncbi A database application for pre-processing, storage and comparison of mass spectra derived from patients and controls
    Mark K Titulaer
    Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
    BMC Bioinformatics 7:403. 2006
  4. ncbi Peptide profiling of cerebrospinal fluid by mass spectrometry
    Lennard J Dekker
    Erasmus University Medical Center, Department of Neurology, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Expert Rev Proteomics 3:297-309. 2006
  5. ncbi A mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitors
    Lennard J M Dekker
    Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
    Rapid Commun Mass Spectrom 23:1183-8. 2009
  6. ncbi Ultrafast and high-throughput mass spectrometric assay for therapeutic drug monitoring of antiretroviral drugs in pediatric HIV-1 infection applying dried blood spots
    Roland J W Meesters
    Department of Neurology, Laboratory of Neuro Oncology and Clinical and Cancer Proteomics, University Medical Center Rotterdam Erasmus MC, Dr Molewaterplein 50, Room Ee 1981, 3015 GE Rotterdam, The Netherlands
    Anal Bioanal Chem 398:319-28. 2010
  7. ncbi Serum levels of pregnancy zone protein are elevated in presymptomatic Alzheimer's disease
    Linda Ijsselstijn
    Department of Neurology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Proteome Res 10:4902-10. 2011
  8. ncbi Mass spectrometry analyses of κ and λ fractions result in increased number of complementarity-determining region identifications
    Ingrid Broodman
    Department of Clinical Chemistry, Erasmus Medical Center, Rotterdam, The Netherlands
    Proteomics 12:183-91. 2012
  9. ncbi Ultra-fast analysis of plasma and intracellular levels of HIV protease inhibitors in children: a clinical application of MALDI mass spectrometry
    Jeroen J A van Kampen
    Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 5:e11409. 2010
  10. ncbi An antibody-based biomarker discovery method by mass spectrometry sequencing of complementarity determining regions
    Lennard J M Dekker
    Department of Neurology, Lab Neuro Oncology, Erasmus University Medical Center, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Anal Bioanal Chem 399:1081-91. 2011

Collaborators

Detail Information

Publications21

  1. ncbi Multiple sclerosis-related proteins identified in cerebrospinal fluid by advanced mass spectrometry
    Marcel P Stoop
    Laboratories of Neuro Oncology Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Centre, Rotterdam, The Netherlands
    Proteomics 8:1576-85. 2008
    ..CSF chromogranin A levels were confirmed to be significantly elevated in the MScl group using an ELISA...
  2. ncbi Label-free peptide profiling of Orbitrap™ full mass spectra
    Mark K Titulaer
    Laboratory of Neuro Oncology and Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Center, Dr, Molewaterplein 50, P, O, Box 2040, 3000 CA Rotterdam, The Netherlands
    BMC Res Notes 4:21. 2011
    ..abstract:..
  3. ncbi A database application for pre-processing, storage and comparison of mass spectra derived from patients and controls
    Mark K Titulaer
    Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
    BMC Bioinformatics 7:403. 2006
    ....
  4. ncbi Peptide profiling of cerebrospinal fluid by mass spectrometry
    Lennard J Dekker
    Erasmus University Medical Center, Department of Neurology, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Expert Rev Proteomics 3:297-309. 2006
    ..This review will discuss the current status of profiling cerebrospinal fluid using mass spectrometry-based techniques, and new developments in this area...
  5. ncbi A mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitors
    Lennard J M Dekker
    Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
    Rapid Commun Mass Spectrom 23:1183-8. 2009
    ....
  6. ncbi Ultrafast and high-throughput mass spectrometric assay for therapeutic drug monitoring of antiretroviral drugs in pediatric HIV-1 infection applying dried blood spots
    Roland J W Meesters
    Department of Neurology, Laboratory of Neuro Oncology and Clinical and Cancer Proteomics, University Medical Center Rotterdam Erasmus MC, Dr Molewaterplein 50, Room Ee 1981, 3015 GE Rotterdam, The Netherlands
    Anal Bioanal Chem 398:319-28. 2010
    ..DBS is perfectly suited for TDM of HIV-1-infected children; but nevertheless, DBS can also easily be applied for TDM of patients in areas with limited or no laboratory facilities...
  7. ncbi Serum levels of pregnancy zone protein are elevated in presymptomatic Alzheimer's disease
    Linda Ijsselstijn
    Department of Neurology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Proteome Res 10:4902-10. 2011
    ..Immunohistochemical validation of the findings on brain tissue sections showed strong PZP expression in senile plaques and in microglial and glial cells in AD with only low expression in some scattered glial cells in controls...
  8. ncbi Mass spectrometry analyses of κ and λ fractions result in increased number of complementarity-determining region identifications
    Ingrid Broodman
    Department of Clinical Chemistry, Erasmus Medical Center, Rotterdam, The Netherlands
    Proteomics 12:183-91. 2012
    ..The number of CDRs and κ:λ ratio was statistically similar in both cases and controls. Molecular dissection of IgG identifies significantly more CDRs, which increases the likelihood of finding lung cancer-related CDR sequences...
  9. ncbi Ultra-fast analysis of plasma and intracellular levels of HIV protease inhibitors in children: a clinical application of MALDI mass spectrometry
    Jeroen J A van Kampen
    Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 5:e11409. 2010
    ....
  10. ncbi An antibody-based biomarker discovery method by mass spectrometry sequencing of complementarity determining regions
    Lennard J M Dekker
    Department of Neurology, Lab Neuro Oncology, Erasmus University Medical Center, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands
    Anal Bioanal Chem 399:1081-91. 2011
    ..In conclusion, specific CDR peptides could be detected and sequenced at relatively low levels (attomole-femtomole range) which should allow the detection of clinically relevant CDR peptides in patient samples...
  11. ncbi Immune responses are characterized by specific shared immunoglobulin peptides that can be detected by proteomic techniques
    Martijn M VanDuijn
    Department of Neurology, Erasmus MC, Rotterdam 3000 CA, The Netherlands
    J Biol Chem 285:29247-53. 2010
    ..This finding implies that immunoglobulin peptides could indeed be a powerful and easily accessible class of biomarkers...
  12. ncbi Serum clusterin levels are not increased in presymptomatic Alzheimer's disease
    Linda Ijsselstijn
    Department of Neurology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Proteome Res 10:2006-10. 2011
    ..Results of the assay showed no significant difference in clusterin levels between presymptomatic AD and controls (p-value 0.54). In conclusion, serum clusterin is not an early, presymptomatic biomarker for AD...
  13. ncbi Optimized nLC-MS workflow for laser capture microdissected breast cancer tissue
    René B H Braakman
    Department of Medical Oncology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Proteomics 75:2844-54. 2012
    ..This article is part of a Special Issue entitled: Proteomics: The clinical link...
  14. ncbi Differential expression of protease activity in serum samples of prostate carcinoma patients with metastases
    Lennard J M Dekker
    Department of Neurology, Erasmus Medical Center, Rotterdam, The Netherlands
    Proteomics 10:2348-58. 2010
    ..Importantly, the observed prostate carcinoma-related inhibition of the proteolysis was reproduced ex vivo using synthetic peptides...
  15. ncbi Sequencing and quantifying IgG fragments and antigen-binding regions by mass spectrometry
    Dominique de Costa
    Department of Pulmonology, Erasmus Medical Center, Rotterdam, The Netherlands
    J Proteome Res 9:2937-45. 2010
    ..From these results, we conclude that the identification of a CDR signature as biomarker for autoimmune diseases and cancer without prior knowledge of the antigen is feasible...
  16. ncbi Proteomic analyses of the developing chicken cardiovascular system
    Els Bon
    Laboratories of Neuro Oncology and Clinical and Cancer Proteomics, Department of Neurology, Erasmus MC, The Netherlands
    J Proteome Res 9:268-74. 2010
    ..We showed that myosin 6 is highly regulated in a stage and heart tissue specific manner. In conclusion, this method can be used to study changes in protein levels of chicken heart tissue in a spatiotemporal manner...
  17. ncbi Identification of glioma neovascularization-related proteins by using MALDI-FTMS and nano-LC fractionation to microdissected tumor vessels
    Dana A N Mustafa
    Department of Pathology, Erasmus Medical Center, Dr Molewaterplein 50, 3015 GD Rotterdam, The Netherlands
    Mol Cell Proteomics 6:1147-57. 2007
    ..This work proves that gel-free mass spectrometric techniques can be used on relatively small numbers of cells generated by microdissection procedures to successfully identify differentially expressed proteins...
  18. ncbi Depletion of high-abundance proteins from serum by immunoaffinity chromatography: a MALDI-FT-MS study
    Lennard J Dekker
    Department of Neurology, ErasmusMC, Rotterdam, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 847:65-9. 2007
    ..From this study we conclude that the depletion of the 250 serum samples was complete and reproducible over a period of 6 weeks...
  19. ncbi FTMS and TOF/TOF mass spectrometry in concert: identifying peptides with high reliability using matrix prespotted MALDI target plates
    Lennard J Dekker
    Laboratory for Neuro Oncology, Department of Neurology, Room AE312, Erasmus Medical Center, P O Box 1738, 3000DR Rotterdam, The Netherlands
    J Chromatogr B Analyt Technol Biomed Life Sci 847:62-4. 2007
    ..By this procedure accurate FTMS mass measurements and TOF/TOF data are obtained from the same spot. We have found that this combination of techniques leads to more reliable identification of peptides...
  20. ncbi MALDI-TOF mass spectrometry analysis of cerebrospinal fluid tryptic peptide profiles to diagnose leptomeningeal metastases in patients with breast cancer
    Lennard J Dekker
    Laboratory of Neuro-Oncology, Department of Neurology, Dr Molewaterplein 40, 3015 GD, Erasmus MC, Rotterdam, The Netherlands
    Mol Cell Proteomics 4:1341-9. 2005
    ..The same method can be used to develop diagnostic assays for other neurological disorders...
  21. ncbi A new method to analyze matrix-assisted laser desorption/ionization time-of-flight peptide profiling mass spectra
    Lennard J Dekker
    Department of Neurology, Erasmus MC, Rotterdam, The Netherlands
    Rapid Commun Mass Spectrom 19:865-70. 2005
    ..This approach will aid the analysis of large numbers of mass spectra of patient samples in a reproducible way for the detection and validation of candidate biomarkers...