M R Mashego

Summary

Affiliation: Delft University of Technology
Country: The Netherlands

Publications

  1. ncbi request reprint Critical evaluation of sampling techniques for residual glucose determination in carbon-limited chemostat culture of Saccharomyces cerevisiae
    M R Mashego
    Kluyver Laboratory for Biotechnology, 67 Julianalaan, 2628BC Delft, The Netherlands
    Biotechnol Bioeng 83:395-9. 2003
  2. ncbi request reprint MIRACLE: mass isotopomer ratio analysis of U-13C-labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites
    M R Mashego
    Kluyver Laboratory for Biotechnology, 67 Julianalaan, 2628BC Delft, The Netherlands
    Biotechnol Bioeng 85:620-8. 2004
  3. ncbi request reprint Changes in the metabolome of Saccharomyces cerevisiae associated with evolution in aerobic glucose-limited chemostats
    Mlawule R Mashego
    Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, 67 Julianalaan, 2628 BC Delft, The Netherlands
    FEMS Yeast Res 5:419-30. 2005
  4. ncbi request reprint In vivo kinetics with rapid perturbation experiments in Saccharomyces cerevisiae using a second-generation BioScope
    Mlawule R Mashego
    Faculty of Applied Sciences, Department of Biotechnology, Technical University of Delft, Julianalaan 67, 2628BC Delft, The Netherlands
    Metab Eng 8:370-83. 2006
  5. ncbi request reprint Microbial metabolomics: past, present and future methodologies
    Mlawule R Mashego
    Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, Delft, The Netherlands
    Biotechnol Lett 29:1-16. 2007
  6. ncbi request reprint Metabolome dynamic responses of Saccharomyces cerevisiae to simultaneous rapid perturbations in external electron acceptor and electron donor
    Mlawule R Mashego
    Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, Julianalaan, Delft, The Netherlands
    FEMS Yeast Res 7:48-66. 2007
  7. ncbi request reprint Quantitative analysis of the microbial metabolome by isotope dilution mass spectrometry using uniformly 13C-labeled cell extracts as internal standards
    Liang Wu
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Anal Biochem 336:164-71. 2005
  8. ncbi request reprint In vivo kinetics of primary metabolism in Saccharomyces cerevisiae studied through prolonged chemostat cultivation
    Liang Wu
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Metab Eng 8:160-71. 2006

Detail Information

Publications8

  1. ncbi request reprint Critical evaluation of sampling techniques for residual glucose determination in carbon-limited chemostat culture of Saccharomyces cerevisiae
    M R Mashego
    Kluyver Laboratory for Biotechnology, 67 Julianalaan, 2628BC Delft, The Netherlands
    Biotechnol Bioeng 83:395-9. 2003
    ....
  2. ncbi request reprint MIRACLE: mass isotopomer ratio analysis of U-13C-labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites
    M R Mashego
    Kluyver Laboratory for Biotechnology, 67 Julianalaan, 2628BC Delft, The Netherlands
    Biotechnol Bioeng 85:620-8. 2004
    ..e., no spiking and standard additions). By coextracting a known amount of U-13C labeled cells with the unlabeled samples, metabolite losses occurring during the sample extraction procedure are corrected for...
  3. ncbi request reprint Changes in the metabolome of Saccharomyces cerevisiae associated with evolution in aerobic glucose-limited chemostats
    Mlawule R Mashego
    Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, 67 Julianalaan, 2628 BC Delft, The Netherlands
    FEMS Yeast Res 5:419-30. 2005
    ..The driving force is proposed to be a growth advantage in the absence of these metabolic overcapacities...
  4. ncbi request reprint In vivo kinetics with rapid perturbation experiments in Saccharomyces cerevisiae using a second-generation BioScope
    Mlawule R Mashego
    Faculty of Applied Sciences, Department of Biotechnology, Technical University of Delft, Julianalaan 67, 2628BC Delft, The Netherlands
    Metab Eng 8:370-83. 2006
    ..It was observed that the dynamic metabolite concentration profiles obtained from both perturbations were nearly the same, with the exception of the C4 metabolites of the TCA cycle, which might be due to differences in culture age...
  5. ncbi request reprint Microbial metabolomics: past, present and future methodologies
    Mlawule R Mashego
    Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, Delft, The Netherlands
    Biotechnol Lett 29:1-16. 2007
    ....
  6. ncbi request reprint Metabolome dynamic responses of Saccharomyces cerevisiae to simultaneous rapid perturbations in external electron acceptor and electron donor
    Mlawule R Mashego
    Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, Julianalaan, Delft, The Netherlands
    FEMS Yeast Res 7:48-66. 2007
    ..Surprisingly, trehalose was not mobilized in the time frame of 180 s...
  7. ncbi request reprint Quantitative analysis of the microbial metabolome by isotope dilution mass spectrometry using uniformly 13C-labeled cell extracts as internal standards
    Liang Wu
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Anal Biochem 336:164-71. 2005
    ..In conclusion, the method presented leads to less workload, more robustness, and a higher precision in metabolome analysis...
  8. ncbi request reprint In vivo kinetics of primary metabolism in Saccharomyces cerevisiae studied through prolonged chemostat cultivation
    Liang Wu
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
    Metab Eng 8:160-71. 2006
    ..Metabolome measurements during the transient indicate that the differences might be due to a reduced ATP regeneration capacity in prolonged cultures...