C A Spek

Summary

Affiliation: Academic Medical Center
Country: The Netherlands

Publications

  1. pmc A low molecular weight heparin inhibits experimental metastasis in mice independently of the endothelial glycocalyx
    Geerte L Van Sluis
    Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    PLoS ONE 5:e11200. 2010
  2. doi request reprint The protein C pathway in cancer metastasis
    C Arnold Spek
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Thromb Res 129:S80-4. 2012
  3. pmc Markers of inflammation and coagulation indicate a prothrombotic state in HIV-infected patients with long-term use of antiretroviral therapy with or without abacavir
    Eefje Jong
    Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The Netherlands
    AIDS Res Ther 7:9. 2010
  4. ncbi request reprint Type I protein C deficiency caused by disruption of a hepatocyte nuclear factor (HNF)-6/HNF-1 binding site in the human protein C gene promoter
    C A Spek
    Hemostasis and Thrombosis Research Center, Department of Hematology, University Hospital, 2300 RC Leiden, The Netherlands
    J Biol Chem 273:10168-73. 1998
  5. ncbi request reprint Tissue factor: from 'just one of the coagulation factors' to a major player in physiology
    C Arnold Spek
    Laboratory for Experimental Internal Medicine, Academic Medical Centre, Amsterdam, The Netherlands
    Blood Coagul Fibrinolysis 15:S3-10. 2004
  6. ncbi request reprint Factor V Leiden and the etiology of inflammatory bowel disease
    C Arnold Spek
    Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Thromb Haemost 98:670-3. 2007
  7. ncbi request reprint Treatment with an anti-CD14 monoclonal antibody delays and inhibits lipopolysaccharide-induced gene expression in humans in vivo
    C Arnold Spek
    Laboratory for Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Clin Immunol 23:132-40. 2003
  8. pmc Unique distance- and DNA-turn-dependent interactions in the human protein C gene promoter confer submaximal transcriptional activity
    C A Spek
    Hemostasis and Thrombosis Research Center, Department of Hematology, University Hospital, Leiden, The Netherlands
    Biochem J 340:513-8. 1999
  9. ncbi request reprint Tissue factor haploinsufficiency during endotoxin induced coagulation and inflammation in mice
    S H H F Schoenmakers
    Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands
    J Thromb Haemost 2:2185-93. 2004
  10. ncbi request reprint Role of the factor V Leiden mutation in septic peritonitis assessed in factor V Leiden transgenic mice
    Lois W Brüggemann
    Laboratory for Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Crit Care Med 34:2201-6. 2006

Detail Information

Publications76

  1. pmc A low molecular weight heparin inhibits experimental metastasis in mice independently of the endothelial glycocalyx
    Geerte L Van Sluis
    Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    PLoS ONE 5:e11200. 2010
    ..The underlying mechanisms are still not clear but it has been suggested that LMWHs (at least in part) limit metastasis by preventing cancer cell-induced destruction of the endothelial glycocalyx...
  2. doi request reprint The protein C pathway in cancer metastasis
    C Arnold Spek
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Thromb Res 129:S80-4. 2012
    ....
  3. pmc Markers of inflammation and coagulation indicate a prothrombotic state in HIV-infected patients with long-term use of antiretroviral therapy with or without abacavir
    Eefje Jong
    Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The Netherlands
    AIDS Res Ther 7:9. 2010
    ..Thirty-three percent of patients with long-term use of antiretroviral treatment had hsCRP levels above 3 mg/L, which is strongly associated with cardiovascular disease in HIV-uninfected individuals...
  4. ncbi request reprint Type I protein C deficiency caused by disruption of a hepatocyte nuclear factor (HNF)-6/HNF-1 binding site in the human protein C gene promoter
    C A Spek
    Hemostasis and Thrombosis Research Center, Department of Hematology, University Hospital, 2300 RC Leiden, The Netherlands
    J Biol Chem 273:10168-73. 1998
    ..It is concluded that HNF-6 is a major determinant of protein C gene activity. Moreover, this is the first report describing the putative involvement of HNF-6 and of an HNF-6 binding site in human pathology...
  5. ncbi request reprint Tissue factor: from 'just one of the coagulation factors' to a major player in physiology
    C Arnold Spek
    Laboratory for Experimental Internal Medicine, Academic Medical Centre, Amsterdam, The Netherlands
    Blood Coagul Fibrinolysis 15:S3-10. 2004
    ..These advances in our knowledge of TF function will improve our understanding of several pathophysiological conditions, and may expand the therapeutic options available for their treatment...
  6. ncbi request reprint Factor V Leiden and the etiology of inflammatory bowel disease
    C Arnold Spek
    Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Thromb Haemost 98:670-3. 2007
    ..We therefore conclude that the FV Leiden allele has no effect in murine colitis and we thus question the importance of activated blood coagulation in the etiology or pathogenesis of IBD...
  7. ncbi request reprint Treatment with an anti-CD14 monoclonal antibody delays and inhibits lipopolysaccharide-induced gene expression in humans in vivo
    C Arnold Spek
    Laboratory for Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Clin Immunol 23:132-40. 2003
    ..These data suggest that peripheral blood cells are of negligible importance in LPS-induced production of inflammatory mediators in vivo and that LPS may activate genes via a CD14-independent pathway that is slower and less efficient...
  8. pmc Unique distance- and DNA-turn-dependent interactions in the human protein C gene promoter confer submaximal transcriptional activity
    C A Spek
    Hemostasis and Thrombosis Research Center, Department of Hematology, University Hospital, Leiden, The Netherlands
    Biochem J 340:513-8. 1999
    ....
  9. ncbi request reprint Tissue factor haploinsufficiency during endotoxin induced coagulation and inflammation in mice
    S H H F Schoenmakers
    Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands
    J Thromb Haemost 2:2185-93. 2004
    ..After 12 h the baseline difference in TF activity was re-established. TF deficiency reduces cytokine production in vitro, but an enhanced induction of TF during murine endotoxemia eliminates this effect in vivo...
  10. ncbi request reprint Role of the factor V Leiden mutation in septic peritonitis assessed in factor V Leiden transgenic mice
    Lois W Brüggemann
    Laboratory for Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Crit Care Med 34:2201-6. 2006
    ..Indeed, both clinical and experimental animal data suggest that a heterozygous FVL genotype might protect against the lethal consequences of sepsis. We sought to confirm the survival advantage of heterozygous FVL mice in septic disease...
  11. pmc Endogenous activated protein C is essential for immune-mediated cancer cell elimination from the circulation
    G L van Sluis
    Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Cancer Lett 306:106-10. 2011
    ..Overall, we thus show that endogenous APC is essential for immune-mediated cancer cell elimination...
  12. ncbi request reprint Role of coagulation FVIII in septic peritonitis assessed in hemophilic mice
    S H H F Schoenmakers
    Laboratory for Experimental Internal Medicine, Academic Medical Center Amsterdam, Amsterdam, The Netherlands
    J Thromb Haemost 3:2738-44. 2005
    ..The role of the intrinsic pathway of coagulation in the pathogenesis of sepsis has not been explored yet...
  13. ncbi request reprint Microvascular coagulopathy and disseminated intravascular coagulation
    H ten Cate
    Academic Medical Center, and the Department of Internal Medicine, Slotervaat Hospital, Amsterdam, The Netherlands
    Crit Care Med 29:S95-7; discussion S97-8. 2001
    ..This may be a critical element in the assessment of ischemia-reperfusion effects of specific anticoagulant therapy...
  14. doi request reprint TF:FVIIa-specific activation of CREB upregulates proapoptotic proteins via protease-activated receptor-2
    H H Versteeg
    Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands
    J Thromb Haemost 6:1550-7. 2008
    ..Our previous findings indicated that the TF:FVIIa complex activates signal transducer and activator of transcription (STAT) signaling, leading to cell survival in TF-transfected baby hamster kidney (BHK) cells...
  15. pmc Inhalation of activated protein C inhibits endotoxin-induced pulmonary inflammation in mice independent of neutrophil recruitment
    S H Slofstra
    Center for Experimental and Molecular Medicine, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
    Br J Pharmacol 149:740-6. 2006
    ..In this study, we therefore assessed the effect of inhaled rhAPC in a murine model of acute lung injury...
  16. ncbi request reprint Regulation of the p21Ras-MAP kinase pathway by factor VIIa
    H H Versteeg
    Laboratory for Experimental Internal Medicine, Academic Medical Centre, Amsterdam, The Netherlands
    J Thromb Haemost 1:1012-8. 2003
    ....
  17. ncbi request reprint Low molecular weight heparin attenuates multiple organ failure in a murine model of disseminated intravascular coagulation
    Sjoukje H Slofstra
    Laboratory for Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Crit Care Med 33:1365-70. 2005
    ..The aim of this study was to document potential beneficial effects of low molecular weight heparin (LMWH) in a lipopolysaccharide-induced DIC model...
  18. doi request reprint Differential effects of anticoagulants on tumor development of mouse cancer cell lines B16, K1735 and CT26 in lung
    Tatjana M H Niers
    Department of Medical Oncology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, Amsterdam, The Netherlands
    Clin Exp Metastasis 26:171-8. 2009
    ..Fibrin content of the tumors was not affected by LMWH. It is concluded that effects of anticoagulants are dependent on cancer cell type and are correlated with their CD24 and PAR-1 expression...
  19. ncbi request reprint Colon cancer metastasis in mouse liver is not affected by hypercoagulability due to Factor V Leiden mutation
    C P W Klerk
    Academic Medical Center, Department of Cell Biology and Histology, University of Amsterdam, The Netherlands
    J Cell Mol Med 11:561-8. 2007
    ..Fibrin deposition and thus inhibition of fibrin formation by anticoagulants do not seem to affect tumour development in this model...
  20. ncbi request reprint The pleiotropic effects of tissue factor: a possible role for factor VIIa-induced intracellular signalling?
    H H Versteeg
    Laboratory for Experimental Internal Medicine, Academic Medical Centre, Amsterdam, The Netherlands
    Thromb Haemost 86:1353-9. 2001
    ..How this progress in the understanding of TF associated signal transduction may generate answers as to the mechanism through which TF exerts it pleiotropic effects will be focus of this review...
  21. ncbi request reprint Local activation of the tissue factor-factor VIIa pathway in patients with pneumonia and the effect of inhibition of this pathway in murine pneumococcal pneumonia
    Anita W Rijneveld
    Center of Infection and Immunity Amsterdam, Laboratory of Experimental Internal Medicine, Department of Pulmonology, University of Amsterdam, The Netherlands
    Crit Care Med 34:1725-30. 2006
    ..The objective of this study was to determine the role of TF-FVIIa in pneumonia caused by Streptococcus pneumoniae, the most important causative organism in community-acquired pneumonia and a major cause of sepsis...
  22. ncbi request reprint Hyperglycemia accelerates arterial thrombus formation and attenuates the antithrombotic response to endotoxin in mice
    Hjalmar R Hansen
    Laboratory for Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Blood Coagul Fibrinolysis 18:627-36. 2007
    ..This reduced rate of thrombus formation was attenuated by hyperglycemia. Together, these data establish a discrete but clear contribution of hyperglycemia in experimental arterial thrombosis...
  23. doi request reprint Coagulation factor Xa signaling: the link between coagulation and inflammatory bowel disease?
    Keren Borensztajn
    Department of Cell Biology, University of Groningen, Antonius Deusinglaan 1, NL 9713 EZ, Groningen, The Netherlands
    Trends Pharmacol Sci 30:8-16. 2009
    ..Here, we propose that FXa-induced PAR(2) activation has an important role in orchestrating intestinal inflammatory and fibroproliferative responses, and that PAR(2) might provide a novel therapeutic target for the management of IBD...
  24. pmc Factor Xa stimulates proinflammatory and profibrotic responses in fibroblasts via protease-activated receptor-2 activation
    Keren Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef 9, NL 1105 AZ, Amsterdam, The Netherlands
    Am J Pathol 172:309-20. 2008
    ..Taken together, these results support the notion that, beyond its role in coagulation, FXa-dependent PAR-2 cleavage might play a role in the progression of tissue fibrosis and remodeling...
  25. doi request reprint Leukotriene synthesis is required for hedgehog-dependent neurite projection in neuralized embryoid bodies but not for motor neuron differentiation
    Maarten F Bijlsma
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Stem Cells 26:1138-45. 2008
    ..These data establish a function for the previously described arachidonic acid-dependent Hh pathway in a developmentally relevant model system...
  26. doi request reprint Hypoxia induces a hedgehog response mediated by HIF-1alpha
    Maarten F Bijlsma
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    J Cell Mol Med 13:2053-60. 2009
    ..In conclusion, we have established that hypoxia is translated into a hedgehog response through HIF-1alpha and this mechanism is likely to be responsible for the hedgehog response observed in various ischaemia models...
  27. doi request reprint Factor Xa: at the crossroads between coagulation and signaling in physiology and disease
    Keren Borensztajn
    Department of Cell Biology, University of Groningen, Antonius Deusinglaan 1, NL 9713 EZ, Groningen, The Netherlands
    Trends Mol Med 14:429-40. 2008
    ....
  28. doi request reprint Endogenous hedgehog expression contributes to myocardial ischemia-reperfusion-induced injury
    Maarten F Bijlsma
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands
    Exp Biol Med (Maywood) 233:989-96. 2008
    ..Thus, Hh seems to exert a dualistic action in cardiac ischemia in which high exogenous levels are able to foster tissue repair and endogenous Hh seems to be deleterious...
  29. pmc Differential gene expression changes in children with severe dengue virus infections
    Martijn D de Kruif
    Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The Netherlands
    PLoS Negl Trop Dis 2:e215. 2008
    ..This study aimed to define differential immune responses in association with clinical outcomes by gene expression profiling of a selected panel of inflammatory genes in whole blood samples from children with severe dengue infections...
  30. doi request reprint Protease-activated receptors, apoptosis and tumor growth
    Keren S Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Pathophysiol Haemost Thromb 36:137-47. 2008
    ..In addition, we discuss the relationship between blood coagulation and cancer biology focusing on the potential role of PAR-induced modulation of cell survival, apoptosis and tumor growth...
  31. ncbi request reprint The role of coagulation in chronic inflammatory disorders: a jack of all trades
    Keren S Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef 9, NL 1105 AZ, Amsterdam, The Netherlands
    Curr Pharm Des 17:9-16. 2011
    ....
  32. pmc Protease-activated receptor-2 induces myofibroblast differentiation and tissue factor up-regulation during bleomycin-induced lung injury: potential role in pulmonary fibrosis
    Keren Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef 9, NL 1105 AZ, Amsterdam, The Netherlands
    Am J Pathol 177:2753-64. 2010
    ..Taken together, these results suggest an important role for PAR-2 in the development of pulmonary fibrosis, and the inhibition of the PAR-2-coagulation axis may provide a novel therapeutic approach to treat this devastating disease...
  33. doi request reprint The role of activated protein C in cancer progression
    Geerte L Van Sluis
    Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Thromb Res 125:S138-42. 2010
    ..It discusses the APC pathway as a potential novel target to influence cancer progression, but it also points to several limitations of APC administration in the setting of cancer cell metastasis...
  34. doi request reprint Coagulation Factor Xa inhibits cancer cell migration via LIMK1-mediated cofilin inactivation
    Keren Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef 9, NL 1105 AZ, Amsterdam, The Netherlands
    Thromb Res 125:e323-8. 2010
    ..Overall we confirm that, beyond its role in blood coagulation, FXa plays a key role in cell migration and we unravel a new mechanism of PAR-1-mediated inhibition of migration via Rho and Src dependent pathways...
  35. doi request reprint Additional value of procalcitonin for diagnosis of infection in patients with fever at the emergency department
    Martijn D de Kruif
    Department of Internal Medicine, Slotervaart Hospital, Amsterdam, The Netherlands
    Crit Care Med 38:457-63. 2010
    ..Second, to create a simple scoring rule implementing PCT values. Third, to determine and compare associations of CRP and PCT with clinical outcomes...
  36. doi request reprint PTX3 predicts severe disease in febrile patients at the emergency department
    Martijn D de Kruif
    Department of Internal Medicine, Slotervaart Hospital, Louwesweg 6, 1066 EC, Amsterdam, The Netherlands
    J Infect 60:122-7. 2010
    ..In order to identify patients warranting critical care as quickly as possible, we investigated the value of PTX3 as a biomarker for disease severity in patients presenting with fever at the emergency department...
  37. pmc Endogenous activated protein C limits cancer cell extravasation through sphingosine-1-phosphate receptor 1-mediated vascular endothelial barrier enhancement
    Geerte L Van Sluis
    Department of Vascular Medicine, Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Blood 114:1968-73. 2009
    ..In conclusion, endogenous APC limits cancer cell extravasation because of S(1)P(1)-mediated VE-cadherin-dependent vascular barrier enhancement...
  38. doi request reprint C-reactive protein elicits white blood cell activation in humans
    Radjesh J Bisoendial
    Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Am J Med 122:582.e1-9. 2009
    ..We tested in humans whether activation of peripheral leukocytes in reaction to the administration of recombinant human C-reactive protein (rhCRP) may provide a mechanism for infectious diseases to promote atherosclerotic disease...
  39. doi request reprint FXa-induced intracellular signaling links coagulation to neoangiogenesis: potential implications for fibrosis
    Keren Borensztajn
    Department of Cell Biology, University Medical Center Groningen, University of Groningen, A Deusinglaan 1, NL 9713 AV Groningen, The Netherlands
    Biochim Biophys Acta 1793:798-805. 2009
    ..Overall, our results establish a link between the coagulation cascade and angiogenesis during fibrosis...
  40. doi request reprint Coagulation factor Xa inhibits cancer cell migration via protease-activated receptor-1 activation
    Keren Borensztajn
    Department of Cell Biology, University of Groningen, Groningen, The Netherlands
    Thromb Res 124:219-25. 2009
    ..These results suggest that, beyond its role in blood coagulation, FXa plays a key role in cancer cell migration. They also shed light on an unexpected role of PAR-1, which appears to be a Janus-like receptor in cancer cell biology...
  41. doi request reprint The coagulation factor Xa/protease activated receptor-2 axis in the progression of liver fibrosis: a multifaceted paradigm
    Keren Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef, Amsterdam, The Netherlands
    J Cell Mol Med 14:143-53. 2010
    ..The current review focuses on blood coagulation factor Xa and summarizes the variety of cellular functions induced by factor Xa-driven PAR-2 activation and the subsequent consequences for tissue repair and hepatic fibrosis...
  42. doi request reprint Blood coagulation factor Xa as an emerging drug target
    Keren Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef 9, NL 1105 AZ, Amsterdam, The Netherlands
    Expert Opin Ther Targets 15:341-9. 2011
    ..This pleiotropic role of FXa forms the basis for its potential contribution to the pathogenesis of several diseases...
  43. ncbi request reprint Sonic hedgehog induces transcription-independent cytoskeletal rearrangement and migration regulated by arachidonate metabolites
    Maarten F Bijlsma
    Center for Experimental and Molecular Medicine, Room G2 105, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Cell Signal 19:2596-604. 2007
    ....
  44. ncbi request reprint Antiinflammatory effects of salmeterol after inhalation of lipopolysaccharide by healthy volunteers
    Nico A Maris
    Department of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, The Netherlands
    Am J Respir Crit Care Med 172:878-84. 2005
    ..Salmeterol is a beta2-adrenoreceptor agonist used in the treatment of obstructive pulmonary disease. Salmeterol inhibits inflammatory responses by neutrophils and mononuclear cells in vitro and in mouse models of lung inflammation in vivo...
  45. pmc Expression profiling via novel multiplex assay allows rapid assessment of gene regulation in defined signalling pathways
    Eric Eldering
    Department of Experimental Immunology, Academical Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Nucleic Acids Res 31:e153. 2003
    ..This can be an advantage compared to hypothesis-free whole genome screens where large numbers of differentially expressed genes can obscure functional interpretation...
  46. pmc Repression of smoothened by patched-dependent (pro-)vitamin D3 secretion
    Maarten F Bijlsma
    Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Meibergdreef, Amsterdam, Netherlands
    PLoS Biol 4:e232. 2006
    ....
  47. ncbi request reprint High throughput mRNA profiling highlights associations between myocardial infarction and aberrant expression of inflammatory molecules in blood cells
    Stephanie Bezzina Wettinger
    Laboratory for Experimental Internal Medicine, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Blood 105:2000-6. 2005
    ..These data support the hypothesis that an inflammatory response involving leucocytes plays a role in the pathogenesis of myocardial infarction...
  48. ncbi request reprint Disseminated intravascular coagulation
    Sjoukje H Slofstra
    Laboratory of Experimental Internal Medicine, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
    Hematol J 4:295-302. 2003
    ..Dissecting the molecular interactions at the onset and progression of DIC might therefore help to elucidate the fundamental consequences of DIC, possibly contributing to better diagnostic tools and more effective therapeutic options...
  49. pmc Tissue factor and cancer metastasis: the role of intracellular and extracellular signaling pathways
    Henri H Versteeg
    Laboratory of Experimental Internal Medicine, Academic Medical Center, Meibergdreef 9, 1106 AZ Amsterdam, The Netherlands
    Mol Med 10:6-11. 2004
    ..In this review, we will discuss these phenomena in more detail and hypothesize on their role in TF-driven metastasis...
  50. ncbi request reprint Ethyl pyruvate exerts combined anti-inflammatory and anticoagulant effects on human monocytic cells
    Marieke A D van Zoelen
    Center for Infection and Immunity Amsterdam CINIMA, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Thromb Haemost 96:789-93. 2006
    ..These results demonstrate for the first time that EP is a compound with combined anti-inflammatory and anticoagulant effects...
  51. ncbi request reprint Coagulation factor Xa drives tumor cells into apoptosis through BH3-only protein Bim up-regulation
    Keren S Borensztajn
    Center for Experimental and Molecular Medicine, Academic Medical Center, Meibergdreef 9, Amsterdam, The Netherlands
    Exp Cell Res 313:2622-33. 2007
    ..We thus provide evidence that beyond its role in blood coagulation, FXa plays a key role in cellular processes in which Bim is the central player in determining cell survival...
  52. ncbi request reprint Inhibition of the tissue factor/factor VIIa pathway does not influence the inflammatory or antibacterial response to abdominal sepsis induced by Escherichia coli in mice
    Sebastian Weijer
    Laboratory of Experimental Internal Medicine and Department of Infectious Diseases, Tropical Medicine, and AIDS, University of Amsterdam, The Netherlands
    J Infect Dis 189:2308-17. 2004
    ..Inhibition of the tissue factor (TF)/factor (F) VIIa pathway has been shown to attenuate the activation of coagulation and to prevent death in a primate model of sepsis caused by gram-negative bacteria...
  53. ncbi request reprint Gene expression profiling identifies C/EBPdelta as a candidate regulator of endotoxin-induced disseminated intravascular coagulation
    Sjoukje H Slofstra
    Center for Experimental and Molecular Medicine, G2 132, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Am J Respir Crit Care Med 176:602-9. 2007
    ....
  54. ncbi request reprint Effects of IC14, an anti-CD14 antibody, on coagulation and fibrinolysis during low-grade endotoxemia in humans
    Annelies Verbon
    Department of Infectious Diseases, Tropical Medicine, and AIDS, Academic Medical Center, University of Amsterdam, The Netherlands
    J Infect Dis 187:55-61. 2003
    ..These results suggest that different hemostatic responses during endotoxemia may proceed via CD14-dependent and -independent pathways...
  55. ncbi request reprint FVIIa:TF induces cell survival via G12/G13-dependent Jak/STAT activation and BclXL production
    Henri H Versteeg
    Laboratory of Experimental Internal Medicine, G2 131, Academic Medical Center, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands
    Circ Res 94:1032-40. 2004
    ..Finally, we demonstrated for the first time that TF/FVIIa-signal transduction is dependent on G12/G13 class G proteins...
  56. doi request reprint Gene expression profiles in murine influenza pneumonia
    Mark C Dessing
    Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Innate Immun 1:366-75. 2009
    ..Many in vitro studies have focused on gene expression in influenza-infected leukocytes, lung tissue or cell lines. However, knowledge of in vivo gene expression in these compartments is limited...
  57. ncbi request reprint Type I diabetes: a role for tissue factor in pancreatic islet transplantation?
    Maikel P Peppelenbosch
    Laboratory for Experimental Internal Medicine, Academic Medical Centre, NL 1105 AZ Amsterdam, Netherlands
    Lancet 360:1999-2000. 2002
  58. ncbi request reprint Blood cell-derived tissue factor influences host response during murine endotoxemia
    Saskia H H F Schoenmakers
    Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Blood Cells Mol Dis 32:325-33. 2004
    ..In summary, our data clearly indicate that endotoxin-induced coagulation and inflammation are strongly influenced by blood cell-derived tissue factor...
  59. ncbi request reprint Coagulation factors VIIa and Xa inhibit apoptosis and anoikis
    Henri H Versteeg
    Laboratory for Experimental Internal Medicine, G2 105, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Oncogene 23:410-7. 2004
    ..Finally, TF:FVIIa-induced FXa generation dramatically increased cell survival. We propose that FVIIa-induced cell survival may explain why overexpression of TF is associated with successful metastasis...
  60. ncbi request reprint Toll-like receptor mRNA levels in alveolar macrophages after inhalation of endotoxin
    N A Maris
    Center of Infection and Immunity Amsterdam CINIMA, University of Amsterdam, The Netherlands
    Eur Respir J 28:622-6. 2006
    ..In conclusion, lipopolysaccharide differentially influences the toll-like receptor mRNA expression profile in human alveolar macrophages in vivo...
  61. doi request reprint Gene expression profile comparison of Barrett's esophagus epithelial cell cultures and biopsies
    J W P M van Baal
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Dis Esophagus 21:628-33. 2008
    ....
  62. ncbi request reprint Tissue factor signal transduction in angiogenesis
    Henri H Versteeg
    Laboratory for Experimental Internal Medicine, G2 130, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Carcinogenesis 24:1009-13. 2003
    ..The recent evidence for this emerging insight will be the subject of this review...
  63. ncbi request reprint Hedgehog: an unusual signal transducer
    Maarten F Bijlsma
    Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Bioessays 26:387-94. 2004
    ..These atypical features make Hedgehog physiology unique in biology and may explain why this field has attracted such significant attention...
  64. ncbi request reprint Protease-activated receptor-4 inhibition protects from multiorgan failure in a murine model of systemic inflammation
    Sjoukje H Slofstra
    Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Meibregdreef 9, 1105 AZ Amsterdam, The Netherlands
    Blood 110:3176-82. 2007
    ..These results uncover an important role for PAR-4 in the Shwartzman reaction and suggest that inhibition of PAR-4 signaling in neutrophils could be protective in systemic inflammation and DIC...
  65. ncbi request reprint Nuclear receptors Nur77, Nurr1, and NOR-1 expressed in atherosclerotic lesion macrophages reduce lipid loading and inflammatory responses
    Peter I Bonta
    Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, The Netherlands
    Arterioscler Thromb Vasc Biol 26:2288-94. 2006
    ..In this study, we investigated expression and function of the NR4A nuclear receptor family, comprising Nur77 (NR4A1, TR3), Nurr1 (NR4A2), and NOR-1 (NR4A3) in human macrophages...
  66. ncbi request reprint Blood coagulation factors as inflammatory mediators
    Saskia H H F Schoenmakers
    Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Blood Cells Mol Dis 34:30-7. 2005
    ..This overview presents current insight in how the main coagulation factors might induce inflammation...
  67. doi request reprint Experimental melanoma metastasis in lungs of mice with congenital coagulation disorders
    Lois W Brüggemann
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    J Cell Mol Med 12:2622-7. 2008
    ....
  68. doi request reprint Protease-activated receptor-2 induces migration of pancreatic cancer cells in an extracellular ATP-dependent manner
    K Shi
    Center for Experimental and Molecular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    J Thromb Haemost 11:1892-902. 2013
    ..However, the role of PAR-2 in the setting of pancreatic cancer remains largely unexplored...
  69. pmc Gross deletions/duplications in PROS1 are relatively common in point mutation-negative hereditary protein S deficiency
    Maria C Pintao
    Einthoven Laboratory for Experimental Vascular Medicine, C2 143, Department of Thrombosis and Haemostasis, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Hum Genet 126:449-56. 2009
    ....
  70. ncbi request reprint Signal transduction induced by activated protein C: no role in protection against sepsis?
    Sjoukje H Slofstra
    Center for Experimental and Molecular Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
    Trends Mol Med 12:374-81. 2006
    ..Here, we review the role of APC in the body natural defense against sepsis and discuss the mechanism by which APC might act at a cellular level...
  71. ncbi request reprint Functional thrombomodulin deficiency causes enhanced thrombus growth in a murine model of carotid artery thrombosis
    Janine Dörffler-Melly
    Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Basic Res Cardiol 98:347-52. 2003
    ..These data confirm and extend the finding that a functional deficiency in TM results in enhanced thrombus formation in a murine model of carotid artery thrombosis and support a role for TM defects in arterial thrombotic disease...
  72. ncbi request reprint A comparative analysis by SAGE of gene expression profiles of esophageal adenocarcinoma and esophageal squamous cell carcinoma
    Jantine W P M van Baal
    Center for Experimental and Molecular Medicine, Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands
    Cell Oncol 30:63-75. 2008
    ..The results could suggest that the BE expression profile is closer related to normal squamous esophagus then to EA. In addition, several uniquely expressed genes are identified...
  73. ncbi request reprint Hedgehog turns lipoproteins into Janus-faced particles
    Maarten F Bijlsma
    Academic Medical Center, Laboratory of Experimental Internal Medicine, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
    Trends Cardiovasc Med 16:217-20. 2006
    ..No longer would LDL merely be bad news for the progression of atherosclerotic plaques, as it also conveys a revascularization and tissue salvage signal in the form of hedgehog...
  74. doi request reprint High factor VIIa levels do not promote tumor metastasis
    Lois W Brüggemann
    Thromb Haemost 99:787-8. 2008
  75. ncbi request reprint A dual role for 7-dehydrocholesterol reductase in regulating Hedgehog signalling?
    Maarten F Bijlsma
    Development 133:3951; author reply 3952-3. 2006
  76. ncbi request reprint In silico tissue factor analysis: a bit-to-bit comparison
    Maikel P Peppelenbosch
    Thromb Haemost 89:592-3. 2003