Jenneke Klein-Nulend

Summary

Affiliation: Academic Centre for Dentistry Amsterdam
Country: The Netherlands

Publications

  1. ncbi request reprint Nitric oxide signaling in mechanical adaptation of bone
    J Klein-Nulend
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3004, 1081, LA, Amsterdam, The Netherlands
    Osteoporos Int 25:1427-37. 2014
  2. doi request reprint Mechanosensation and transduction in osteocytes
    Jenneke Klein-Nulend
    Department of Oral Cell Biology, ACTA VU University Amsterdam, Research Institute MOVE, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands
    Bone 54:182-90. 2013
  3. ncbi request reprint Mechanical loading and how it affects bone cells: the role of the osteocyte cytoskeleton in maintaining our skeleton
    J Klein-Nulend
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Eur Cell Mater 24:278-91. 2012
  4. ncbi request reprint Release of nitric oxide, but not prostaglandin E2, by bone cells depends on fluid flow frequency
    Margriet G Mullender
    ACTA Vrije Universiteit, Department of Oral Cell Biology, Van der Boechorststraat 7, NL 1081 BT Amsterdam, The Netherlands
    J Orthop Res 24:1170-7. 2006
  5. doi request reprint Fluoride inhibits the response of bone cells to mechanical loading
    Hubertine M E Willems
    Department of Oral Cell Biology, ACTA VU University Amsterdam, Research Institute MOVE, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands
    Odontology 99:112-8. 2011
  6. ncbi request reprint A novel approach revealing the effect of a collagenous membrane on osteoconduction in maxillary sinus floor elevation with β-tricalcium phosphate
    Engelbert A J M Schulten
    Department of Oral and Maxillofacial Surgery, VU University Medical Centre ACTA, Research Institute MOVE, Amsterdam, The Netherlands
    Eur Cell Mater 25:215-28. 2013
  7. ncbi request reprint Polyamines modulate nitric oxide production and COX-2 gene expression in response to mechanical loading in human adipose tissue-derived mesenchymal stem cells
    Geuranne S Tjabringa
    Department of Oral Cell Biology, Academic Center of Dentistry Amsterdam, Universiteit van Amsterdam, Amsterdam, The Netherlands
    Stem Cells 24:2262-9. 2006
  8. ncbi request reprint Osteocyte morphology in fibula and calvaria --- is there a role for mechanosensing?
    Aviral Vatsa
    Department of Oral Cell Biology, ACTA Universiteit van Amsterdam and Vrije Universiteit, Research Institute MOVE, Amsterdam, The Netherlands
    Bone 43:452-8. 2008
  9. doi request reprint Round versus flat: bone cell morphology, elasticity, and mechanosensing
    Rommel G Bacabac
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam UvA and Vrije Universiteit, Amsterdam, The Netherlands
    J Biomech 41:1590-8. 2008
  10. doi request reprint VDR dependent and independent effects of 1,25-dihydroxyvitamin D3 on nitric oxide production by osteoblasts
    Hubertine M E Willems
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands
    Steroids 77:126-31. 2012

Collaborators

Detail Information

Publications46

  1. ncbi request reprint Nitric oxide signaling in mechanical adaptation of bone
    J Klein-Nulend
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3004, 1081, LA, Amsterdam, The Netherlands
    Osteoporos Int 25:1427-37. 2014
    ..Since mechanical stimuli and NO production enhance bone strength and fracture resistance, these new insights may facilitate the development of novel osteoporosis treatments. ..
  2. doi request reprint Mechanosensation and transduction in osteocytes
    Jenneke Klein-Nulend
    Department of Oral Cell Biology, ACTA VU University Amsterdam, Research Institute MOVE, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands
    Bone 54:182-90. 2013
    ....
  3. ncbi request reprint Mechanical loading and how it affects bone cells: the role of the osteocyte cytoskeleton in maintaining our skeleton
    J Klein-Nulend
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Eur Cell Mater 24:278-91. 2012
    ....
  4. ncbi request reprint Release of nitric oxide, but not prostaglandin E2, by bone cells depends on fluid flow frequency
    Margriet G Mullender
    ACTA Vrije Universiteit, Department of Oral Cell Biology, Van der Boechorststraat 7, NL 1081 BT Amsterdam, The Netherlands
    J Orthop Res 24:1170-7. 2006
    ..This implies that the NO response may play a role in mediating the differential effects of the various loading patterns on bone...
  5. doi request reprint Fluoride inhibits the response of bone cells to mechanical loading
    Hubertine M E Willems
    Department of Oral Cell Biology, ACTA VU University Amsterdam, Research Institute MOVE, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands
    Odontology 99:112-8. 2011
    ..Since decreased mechanosensitivity reduces bone mass, the reported anabolic effect of fluoride on bone mass in vivo is likely mediated by other factors than changed bone cell mechanosensitivity...
  6. ncbi request reprint A novel approach revealing the effect of a collagenous membrane on osteoconduction in maxillary sinus floor elevation with β-tricalcium phosphate
    Engelbert A J M Schulten
    Department of Oral and Maxillofacial Surgery, VU University Medical Centre ACTA, Research Institute MOVE, Amsterdam, The Netherlands
    Eur Cell Mater 25:215-28. 2013
    ....
  7. ncbi request reprint Polyamines modulate nitric oxide production and COX-2 gene expression in response to mechanical loading in human adipose tissue-derived mesenchymal stem cells
    Geuranne S Tjabringa
    Department of Oral Cell Biology, Academic Center of Dentistry Amsterdam, Universiteit van Amsterdam, Amsterdam, The Netherlands
    Stem Cells 24:2262-9. 2006
    ..In conclusion, this is the first study implicating polyamines in the response of human AT-MSCs to mechanical loading, creating opportunities for the use of polyamines in tissue engineering approaches targeting skeletal defects...
  8. ncbi request reprint Osteocyte morphology in fibula and calvaria --- is there a role for mechanosensing?
    Aviral Vatsa
    Department of Oral Cell Biology, ACTA Universiteit van Amsterdam and Vrije Universiteit, Research Institute MOVE, Amsterdam, The Netherlands
    Bone 43:452-8. 2008
    ....
  9. doi request reprint Round versus flat: bone cell morphology, elasticity, and mechanosensing
    Rommel G Bacabac
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam UvA and Vrije Universiteit, Amsterdam, The Netherlands
    J Biomech 41:1590-8. 2008
    ..Our assay provides novel opportunities for in vitro studies under a controlled suspended morphology versus commonly studied adherent morphologies...
  10. doi request reprint VDR dependent and independent effects of 1,25-dihydroxyvitamin D3 on nitric oxide production by osteoblasts
    Hubertine M E Willems
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands
    Steroids 77:126-31. 2012
    ..In conclusion, 1,25(OH)(2)D(3) and mechanical loading interact at the level of mechanotransduction, whereby 1,25(OH)(2)D(3) seems to act independently of VDR genomic mechanism...
  11. doi request reprint Noise enhances the rapid nitric oxide production by bone cells in response to fluid shear stress
    Rommel G Bacabac
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam Universiteit van Amsterdam and Vrije Universiteit, Research Institute MOVE, Amsterdam, The Netherlands
    Technol Health Care 17:57-65. 2009
    ..Since NO regulate bone formation as well as resorption, our results suggest that noise enhances the activity of bone cells in driving the mechanical adaptation of bone...
  12. ncbi request reprint Bone cell responses to high-frequency vibration stress: does the nucleus oscillate within the cytoplasm?
    Rommel G Bacabac
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam Universiteit van Amsterdam, Amsterdam, The Netherlands
    FASEB J 20:858-64. 2006
    ..Correlations of NO and PGE2 with the maximum acceleration rate then relate to nucleus oscillations, providing a physical basis for cellular mechanosensing of high-frequency loading...
  13. doi request reprint Bone cells from patients with quiescent Crohn's disease show a reduced growth potential and an impeded maturation
    Angela E Oostlander
    Department of Endocrinology, VU University Medical Center, Research Institute MOVE, Amsterdam, The Netherlands
    J Cell Biochem 113:2424-31. 2012
    ..These disease-related alterations combined with an unchanged sensitivity of CD patient-derived bone cells to inflammatory cytokines, provide a new insight in the understanding of CD-associated bone loss...
  14. doi request reprint Early activation of the beta-catenin pathway in osteocytes is mediated by nitric oxide, phosphatidyl inositol-3 kinase/Akt, and focal adhesion kinase
    Ana Santos
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Biochem Biophys Res Commun 391:364-9. 2010
    ..These data provide a framework for understanding the role of beta-catenin in mechanical adaptation of bone...
  15. doi request reprint Differences in proliferation, differentiation, and cytokine production by bone cells seeded on titanium-nitride and cobalt-chromium-molybdenum surfaces
    Ruud P van Hove
    Department of Oral Cell Biology, Research Institute MOVE, ACTA University of Amsterdam, The Netherlands
    J Biomater Appl 28:278-87. 2013
    ..05), though interleukin-1β was occasionally detected on both surfaces. These findings suggest improved osteoconduction on titanium-nitride compared with cobalt-chromium-molybdenum surface. ..
  16. ncbi request reprint Osteocytes subjected to fluid flow inhibit osteoclast formation and bone resorption
    S Djien Tan
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam Universiteit van Amsterdam and Vrije Universiteit, Research Institute MOVE, Amsterdam, The Netherlands
    Bone 41:745-51. 2007
    ..Thus, the osteocyte appears to be more responsive to PFF than the osteoblast or periosteal fibroblast regarding to the production of soluble factors affecting osteoclast formation and bone resorption...
  17. doi request reprint Pulsating fluid flow modulates gene expression of proteins involved in Wnt signaling pathways in osteocytes
    Ana Santos
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    J Orthop Res 27:1280-7. 2009
    ..The response to PFF differed in MC3T3-E1 osteoblasts. Because Wnt signaling is important for bone mass regulation, osteocytes might orchestrate loading-induced bone remodeling through, among others, Wnts...
  18. ncbi request reprint Initial stress-kick is required for fluid shear stress-induced rate dependent activation of bone cells
    Rommel G Bacabac
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, Vrije Universiteit, Amsterdam, The Netherlands
    Ann Biomed Eng 33:104-10. 2005
    ..This suggests that high-rate stimuli condition bone cells to be more sensitive for high-frequency, low-amplitude loads...
  19. ncbi request reprint Differential effects of bone morphogenetic protein-2 and transforming growth factor-beta1 on gene expression of collagen-modifying enzymes in human adipose tissue-derived mesenchymal stem cells
    Marlene Knippenberg
    Department of Oral Cell Biology, Academic Centre of Dentistry Amsterdam ACTA, Universiteit van Amsterdam, and Research Institute MOVE, Vrije Universiteit, Amsterdam, The Netherlands
    Tissue Eng Part A 15:2213-25. 2009
    ..AT-MSCs may thus be able to appropriately modify type I collagen to form a functional bone extracellular matrix for tissue engineering, dependent on the growth factor added...
  20. doi request reprint Mechanical loading stimulates BMP7, but not BMP2, production by osteocytes
    Ana Santos
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam ACTA, University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Gustav Mahlerlaan, LA, The Netherlands
    Calcif Tissue Int 89:318-26. 2011
    ..Since BMP7 plays a major role in bone development and remodeling, these data might contribute to a better understanding of the mechanism leading to the mechanical adaptation of bone...
  21. doi request reprint Low-intensity pulsed ultrasound affects RUNX2 immunopositive osteogenic cells in delayed clinical fracture healing
    Sjoerd Rutten
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Bone 45:862-9. 2009
    ..The effect was studied in both atrophic and hypertrophic delayed unions...
  22. pmc Inhibition of osteoclastogenesis by mechanically loaded osteocytes: involvement of MEPE
    Rishikesh N Kulkarni
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands
    Calcif Tissue Int 87:461-8. 2010
    ....
  23. doi request reprint Human maxillary sinus floor elevation as a model for bone regeneration enabling the application of one-step surgical procedures
    Elisabet Farré-Guasch
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, Research Institute MOVE, University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands
    Tissue Eng Part B Rev 19:69-82. 2013
    ..In this review, MSFE is discussed as a valid model to test bone tissue engineering approaches, such as the one-step surgical procedure. This procedure might be applied in other regenerative medicine applications as well...
  24. ncbi request reprint Bio imaging of intracellular NO production in single bone cells after mechanical stimulation
    Aviral Vatsa
    Department of Oral Cell Biology, ACTA Universiteit van Amsterdam, Amsterdam, The Netherlands
    J Bone Miner Res 21:1722-8. 2006
    ..This is done in real time using the fluorescent chromophore DAR-4M AM. Differences in cellular response to mechanical stimulation of different regions of a single cell were observed...
  25. ncbi request reprint Osteocytes subjected to pulsating fluid flow regulate osteoblast proliferation and differentiation
    Peter S Vezeridis
    Department of Oral Cell Biology, Academic Center of Dentistry Amsterdam ACTA, Universiteit van Amsterdam and Vrije Universiteit, Amsterdam, The Netherlands
    Biochem Biophys Res Commun 348:1082-8. 2006
    ..Thus, the osteocyte appears to be more responsive to PFF than the osteoblast or periosteal fibroblast with respect to the production of soluble signaling molecules affecting osteoblast proliferation and differentiation...
  26. ncbi request reprint Extracellular NO signalling from a mechanically stimulated osteocyte
    Aviral Vatsa
    Department of Oral Cell Biology, ACTA Universiteit van Amsterdam, Vrije Universiteit, Research Institute MOVE, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    J Biomech 40:S89-95. 2007
    ....
  27. doi request reprint Mechanical loading prevents the stimulating effect of IL-1β on osteocyte-modulated osteoclastogenesis
    Rishikesh N Kulkarni
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Biochem Biophys Res Commun 420:11-6. 2012
    ..Our results suggest that IL-1β increases osteocyte-modulated osteoclastogenesis, and that mechanical loading of osteocytes may abolish IL-1β-induced osteoclastogenesis...
  28. doi request reprint Osteoblast isolation from murine calvaria and long bones
    Astrid D Bakker
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam ACTA, University of Amsterdam and VU University Amsterdam, Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Methods Mol Biol 816:19-29. 2012
    ..Because of differences in origin and isolation method, each of the primary bone cell cultures described will have their own characteristics...
  29. pmc Short (15 minutes) bone morphogenetic protein-2 treatment stimulates osteogenic differentiation of human adipose stem cells seeded on calcium phosphate scaffolds in vitro
    Janice R Overman
    Department of Oral Cell Biology, Academic Centre of Dentistry Amsterdam, Research Institute MOVE, University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands
    Tissue Eng Part A 19:571-81. 2013
    ..These results strongly support a one-step clinical concept for bone regeneration...
  30. ncbi request reprint Adipose tissue-derived mesenchymal stem cells acquire bone cell-like responsiveness to fluid shear stress on osteogenic stimulation
    Marlene Knippenberg
    Department of Oral Cell Biology, Academic Center of Dentistry Amsterdam, Universiteit van Amsterdam, Amsterdam, The Netherlands
    Tissue Eng 11:1780-8. 2005
    ..ATMSCs might be able to perform bone cell-specific functions during bone (re)modeling in vivo and, therefore, provide a promising new tool for bone tissue engineering...
  31. ncbi request reprint Osteocyte morphology in human tibiae of different bone pathologies with different bone mineral density--is there a role for mechanosensing?
    Ruud P van Hove
    Department of Oral Cell Biology, ACTA University of Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Bone 45:321-9. 2009
    ..The exact relationship between osteocyte morphology and bone architecture, however, is complex and deserves further study...
  32. ncbi request reprint Stem cells from adipose tissue allow challenging new concepts for regenerative medicine
    Marco N Helder
    Department of Orthopedic Surgery, VU University Medical Center VUmc, Amsterdam, The Netherlands
    Tissue Eng 13:1799-808. 2007
    ..The integrated steps can be accomplished within one surgical procedure in a surgical theater. Although the proposed concept has been developed for spinal fusion, potential application in other surgical disciplines is presumed realistic...
  33. doi request reprint Reaming debris as a novel source of autologous bone to enhance healing of bone defects
    Astrid D Bakker
    Department of Oral Cell Biology, ACTA University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    J Biomed Mater Res A 97:457-65. 2011
    ..These results indicate that reaming debris could serve as an excellent alternative to iliac crest bone for speeding up the healing process in bone defects that are treated with an intramedullary nail...
  34. doi request reprint MT1-MMP modulates the mechanosensitivity of osteocytes
    Rishikesh N Kulkarni
    Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Department of Oral Cell Biology, Research Institute MOVE, Amsterdam, The Netherlands
    Biochem Biophys Res Commun 417:824-9. 2012
    ....
  35. doi request reprint Isolation of primary avian osteocytes
    Cor M Semeins
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam ACTA, University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Methods Mol Biol 816:43-53. 2012
    ..The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3...
  36. doi request reprint WNT5A induces osteogenic differentiation of human adipose stem cells via rho-associated kinase ROCK
    Ana Santos
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam ACTA, University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands
    Cytotherapy 12:924-32. 2010
    ..We examined whether Wnt5a induces osteogenic and/or adipogenic differentiation of hASC...
  37. ncbi request reprint Osteocyte and bone structure
    Jenneke Klein-Nulend
    ACTA Vrije Universiteit, Department of Oral Cell Biology, Van der Boechorststraat 7, NL 1081 BT Amsterdam, The Netherlands
    Curr Osteoporos Rep 1:5-10. 2003
    ..Alignment during remodeling seems to occur as a result of the osteocyte's sensing different canalicular flow patterns around the cutting cone and reversal zone during loading, therefore determining the bone's structure...
  38. doi request reprint Endothelial nitric oxide synthase is not essential for nitric oxide production by osteoblasts subjected to fluid shear stress in vitro
    Astrid D Bakker
    Department of Oral Cell Biology, Research Institute MOVE, ACTA University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands
    Calcif Tissue Int 92:228-39. 2013
    ..Our data challenge the notion that eNOS is a key effector of mechanically induced bone maintenance...
  39. ncbi request reprint Dynamic shear stress in parallel-plate flow chambers
    Rommel G Bacabac
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam Vrije Universiteit, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    J Biomech 38:159-67. 2005
    ....
  40. doi request reprint Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes
    Petra Juffer
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam, The Netherlands
    Am J Physiol Endocrinol Metab 302:E389-95. 2012
    ..The abundant expression levels of these factors in muscle cells, in combination with low expression in MLO-Y4 osteocytes, provide a possibility that growth factors expressed in muscle could affect signaling in bone cells...
  41. ncbi request reprint Mechanical loading by fluid shear stress of myotube glycocalyx stimulates growth factor expression and nitric oxide production
    Petra Juffer
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam ACTA, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands
    Cell Biochem Biophys 69:411-9. 2014
    ..These results suggest that shear stress exerted on myofiber extracellular matrix plays an important role in mechanotransduction in muscle. ..
  42. ncbi request reprint Mechanically loaded myotubes affect osteoclast formation
    Petra Juffer
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam ACTA, MOVE Research Institute Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands
    Calcif Tissue Int 94:319-26. 2014
    ..These results suggest that muscle could potentially affect bone homeostasis in vivo via production of growth factors and/or cytokines. ..
  43. doi request reprint Low-intensity pulsed ultrasound increases bone volume, osteoid thickness and mineral apposition rate in the area of fracture healing in patients with a delayed union of the osteotomized fibula
    Sjoerd Rutten
    Department of Oral Cell Biology, ACTA Universiteit van Amsterdam and Vrije Universiteit, Research Institute MOVE, Amsterdam, The Netherlands
    Bone 43:348-54. 2008
    ....
  44. doi request reprint PLS3 mutations in X-linked osteoporosis with fractures
    Fleur S van Dijk
    Department of Clinical Genetics, VU University Medical Center, Amsterdam, The Netherlands
    N Engl J Med 369:1529-36. 2013
    ....
  45. ncbi request reprint Shear stress inhibits while disuse promotes osteocyte apoptosis
    Astrid Bakker
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam Vrije Universiteit, Amsterdam, The Netherlands
    Biochem Biophys Res Commun 320:1163-8. 2004
    ..These data suggest that disuse promotes osteocyte apoptosis, while mechanical stimulation by fluid shear stress promotes osteocyte survival, by modulating the Bcl-2/Bax expression ratio...
  46. ncbi request reprint Nitric oxide production by bone cells is fluid shear stress rate dependent
    Rommel G Bacabac
    Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam Vrije Universiteit, Amsterdam, The Netherlands
    Biochem Biophys Res Commun 315:823-9. 2004
    ..11 Pa-Hz(-1)) and decreased to 0.03 Pa-Hz(-1) at 15 min. We conclude that the fluid shear stress rate is an important parameter for bone cell activation...