V M Christoffels

Summary

Affiliation: Academic Medical Center
Country: The Netherlands

Publications

  1. ncbi request reprint Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart
    V M Christoffels
    Experimental and Molecular Cardiology Group, University of Amsterdam, The Netherlands
    Dev Biol 224:263-74. 2000
  2. doi request reprint Gene regulatory elements of the cardiac conduction system
    Karel van Duijvenboden
    Department of Anatomy, Embryology and Physiology, Academic Medical Centre, L2 108, Meibergdreef 15, 1105 AZ Amsterdam, the Netherlands Tel 31 20 5667821 Fax 31 20 6976177
    Brief Funct Genomics 13:28-38. 2014
  3. pmc Tbx2 and Tbx3 induce atrioventricular myocardial development and endocardial cushion formation
    Reena Singh
    Institut fur Molekularbiologie, Medizinische Hochschule Hannover, Germany
    Cell Mol Life Sci 69:1377-89. 2012
  4. pmc T-box factors determine cardiac design
    W M H Hoogaars
    Heart Failure Research Center, Department of Anatomy and Embryology, Academic Medical Center, Amsterdam, The Netherlands
    Cell Mol Life Sci 64:646-60. 2007
  5. ncbi request reprint Formation of the venous pole of the heart from an Nkx2-5-negative precursor population requires Tbx18
    Vincent M Christoffels
    Department of Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
    Circ Res 98:1555-63. 2006
  6. doi request reprint Development of the pacemaker tissues of the heart
    Vincent M Christoffels
    Heart Failure Research Center, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    Circ Res 106:240-54. 2010
  7. ncbi request reprint Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol
    A F Moorman
    Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
    J Histochem Cytochem 49:1-8. 2001
  8. ncbi request reprint Gene and cluster-specific expression of the Iroquois family members during mouse development
    A C Houweling
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    Mech Dev 107:169-74. 2001
  9. ncbi request reprint Presence of functional sarcoplasmic reticulum in the developing heart and its confinement to chamber myocardium
    A F Moorman
    Experimental and Molecular Cardiology Group, Cardiovascular Research Institute Amsterdam, Academic Medical Center, Amsterdam, 1105 AZ, The Netherlands
    Dev Biol 223:279-90. 2000
  10. pmc The cardiac sodium channel displays differential distribution in the conduction system and transmural heterogeneity in the murine ventricular myocardium
    C A Remme
    Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Basic Res Cardiol 104:511-22. 2009

Collaborators

Detail Information

Publications49

  1. ncbi request reprint Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart
    V M Christoffels
    Experimental and Molecular Cardiology Group, University of Amsterdam, The Netherlands
    Dev Biol 224:263-74. 2000
    ....
  2. doi request reprint Gene regulatory elements of the cardiac conduction system
    Karel van Duijvenboden
    Department of Anatomy, Embryology and Physiology, Academic Medical Centre, L2 108, Meibergdreef 15, 1105 AZ Amsterdam, the Netherlands Tel 31 20 5667821 Fax 31 20 6976177
    Brief Funct Genomics 13:28-38. 2014
    ..Furthermore, the risk variant of the allele abrogated enhancer function in both cases. Functional studies on regulatory DNA will likely receive a boost through recent developments in genome modification technologies. ..
  3. pmc Tbx2 and Tbx3 induce atrioventricular myocardial development and endocardial cushion formation
    Reena Singh
    Institut fur Molekularbiologie, Medizinische Hochschule Hannover, Germany
    Cell Mol Life Sci 69:1377-89. 2012
    ..Tbx2 and Tbx3 trigger development of the cushions through a regulatory feed-forward loop with Bmp2, thus providing a mechanism for the co-localization and coordination of these important processes in heart development...
  4. pmc T-box factors determine cardiac design
    W M H Hoogaars
    Heart Failure Research Center, Department of Anatomy and Embryology, Academic Medical Center, Amsterdam, The Netherlands
    Cell Mol Life Sci 64:646-60. 2007
    ..Insight into the roles of T-box factors in these processes will enhance our understanding of heart formation and the underlying molecular regulatory pathways...
  5. ncbi request reprint Formation of the venous pole of the heart from an Nkx2-5-negative precursor population requires Tbx18
    Vincent M Christoffels
    Department of Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
    Circ Res 98:1555-63. 2006
    ....
  6. doi request reprint Development of the pacemaker tissues of the heart
    Vincent M Christoffels
    Heart Failure Research Center, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    Circ Res 106:240-54. 2010
    ..It will also shed light on the etiology of inborn and acquired errors of nodal tissues...
  7. ncbi request reprint Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol
    A F Moorman
    Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
    J Histochem Cytochem 49:1-8. 2001
    ..The radioactive ISH procedure scarcely permitted detection of these sequences, underscoring the value of this novel method...
  8. ncbi request reprint Gene and cluster-specific expression of the Iroquois family members during mouse development
    A C Houweling
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    Mech Dev 107:169-74. 2001
    ..The results indicate that the Irx genes are coordinately regulated at the level of the cluster...
  9. ncbi request reprint Presence of functional sarcoplasmic reticulum in the developing heart and its confinement to chamber myocardium
    A F Moorman
    Experimental and Molecular Cardiology Group, Cardiovascular Research Institute Amsterdam, Academic Medical Center, Amsterdam, 1105 AZ, The Netherlands
    Dev Biol 223:279-90. 2000
    ..iii) The pattern of SERCA2a expression underscores a manner of chamber development by differentiation at the outer curvature, rather than by segmentation of the linear heart tube...
  10. pmc The cardiac sodium channel displays differential distribution in the conduction system and transmural heterogeneity in the murine ventricular myocardium
    C A Remme
    Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Basic Res Cardiol 104:511-22. 2009
    ..This differential distribution of the cardiac sodium channel may have profound consequences for conduction disease phenotypes and arrhythmogenesis in the setting of sodium channel disease...
  11. ncbi request reprint Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice
    M Campione
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Dev Biol 231:252-64. 2001
    ..Furthermore, altered Pitx2 expression correlated with the occurrence of DORV. Our data reveal the existence of molecular isomerism not only in the atrial, but also in the ventricular compartment of the heart...
  12. doi request reprint A molecular and genetic outline of cardiac morphogenesis
    M S Rana
    Heart Failure Research Center, Department of Anatomy, Embryology and Physiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Acta Physiol (Oxf) 207:588-615. 2013
    ..In this review, we provide an overview of the building plan of the vertebrate four-chambered heart, with a special focus on cardiac progenitor cell specification, differentiation and deployment during arterial pole development...
  13. pmc Sox4 mediates Tbx3 transcriptional regulation of the gap junction protein Cx43
    C J J Boogerd
    Heart Failure Research Centre, Academic Medical Centre, University of Amsterdam, The Netherlands
    Cell Mol Life Sci 68:3949-61. 2011
    ..Mechanistically, we postulate that Sox4 is a mediator of Tbx3 transcriptional activity...
  14. ncbi request reprint Chamber formation and morphogenesis in the developing mammalian heart
    V M Christoffels
    Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, Amsterdam, 1105, The Netherlands
    Dev Biol 223:266-78. 2000
    ....
  15. ncbi request reprint A single regulatory module of the carbamoylphosphate synthetase I gene executes its hepatic program of expression
    V M Christoffels
    Department of Anatomy and Embryology and the Genetically Modified Mice Facility, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Biol Chem 275:40020-7. 2000
    ....
  16. doi request reprint Acquisition of high quality DNA for massive parallel sequencing by in vivo chromatin immunoprecipitation
    M van den Boogaard
    Heart Failure Research Centre, Academic Medical Centre, section K2, Amsterdam, The Netherlands
    Methods Mol Biol 977:53-64. 2013
    ..Although the method makes use of adult mouse hearts, it can, with relative ease, be adapted for the in vivo ChIP isolation of DNA from other cell and tissue sources with the intention of massive parallel sequencing...
  17. ncbi request reprint Effect of amiodarone and dronedarone administration in rats on thyroid hormone-dependent gene expression in different cardiac components
    I Stoykov
    Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The Netherlands
    Eur J Endocrinol 156:695-702. 2007
    ..In view of their different actions on thyroid hormone receptor (TR) isoforms we set out to investigate whether amiodarone (AM) and dronedarone (Dron) have different and/or component-specific effects on cardiac gene expression...
  18. doi request reprint Nuclear recruitment assay as a tool to validate transcription factor interactions in Mammalian cells
    C J J Boogerd
    Heart Failure Research Center, Academic Medical Center, section K2, Amsterdam, The Netherlands
    Methods Mol Biol 977:243-8. 2013
    ..We provide a detailed protocol for an intracellular immunofluorescence technique that may be used as an alternative or complimentary study for transcription factor interaction verification...
  19. ncbi request reprint TBX5 overexpression stimulates differentiation of chamber myocardium in P19C16 embryonic carcinoma cells
    Arnoud C Fijnvandraat
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Muscle Res Cell Motil 24:211-8. 2003
    ..This indicates that Tbx5 enhances cardiac maturation within this system rather than cardiogenesis...
  20. doi request reprint Msx1 and Msx2 are functional interacting partners of T-box factors in the regulation of Connexin43
    Kees Jan Boogerd
    Department of Anatomy and Embryology, Heart Failure Research Centre, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands
    Cardiovasc Res 78:485-93. 2008
    ..Discovering protein partners of Tbx2 and Tbx3 will shed light on the mechanisms by which these factors regulate these gene programs...
  21. ncbi request reprint Cardiomyocytes derived from embryonic stem cells resemble cardiomyocytes of the embryonic heart tube
    Arnoud C Fijnvandraat
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Cardiovasc Res 58:399-409. 2003
    ..To assess whether this program is recapitulated in in vitro differentiated embryonic stem cells, we performed qualitative and quantitative analyses of cardiogenesis in vivo and in vitro...
  22. ncbi request reprint Pitx2c and Nkx2-5 are required for the formation and identity of the pulmonary myocardium
    Mathilda T M Mommersteeg
    Heart Failure Research Center, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Circ Res 101:902-9. 2007
    ..In conclusion, our data suggest a cellular mechanism for pulmonary myocardium formation and highlight the key roles played by Pitx2c and Nkx2-5 in its formation and identity...
  23. pmc The heart-forming fields: one or multiple?
    Antoon F M Moorman
    Heart Failure Research Center, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    Philos Trans R Soc Lond B Biol Sci 362:1257-65. 2007
    ....
  24. doi request reprint TBX3 and its splice variant TBX3 + exon 2a are functionally similar
    Willem M H Hoogaars
    Center for Heart Failure Research, Academic Medical Centre, Amsterdam, The Netherlands
    Pigment Cell Melanoma Res 21:379-87. 2008
    ..The results suggest that in the assays used, Tbx3 and Tbx3 + 2a are functionally equivalent and that like Tbx2, Tbx3 may also function as an anti-senescence factor in melanoma...
  25. doi request reprint A gain-of-function TBX5 mutation is associated with atypical Holt-Oram syndrome and paroxysmal atrial fibrillation
    Alex V Postma
    Heart Failure Research Center, L2 108 1, Academic Medical Center, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Circ Res 102:1433-42. 2008
    ....
  26. doi request reprint Transcription factor Tbx3 is required for the specification of the atrioventricular conduction system
    Martijn L Bakker
    Heart Failure Research Center, Academic Medical Center, Amsterdam, The Netherlands
    Circ Res 102:1340-9. 2008
    ..Our data suggest a mechanism in which Tbx3 represses differentiation into ventricular working myocardium, thereby imposing the conduction system phenotype on cells within its expression domain...
  27. ncbi request reprint Regulatory modules in the developing heart
    Petra E M H Habets
    Experimental and Molecular Cardiology Group, Department of Anatomy and Embryology, Academic Medical Center L2 255, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Cardiovasc Res 58:246-63. 2003
    ..Most of the regulatory modules show a domain of activity broader than the morphological boundary of a cardiac compartment and seem to respond to a patterning program along the antero-posterior axis...
  28. ncbi request reprint Tbx20 is essential for cardiac chamber differentiation and repression of Tbx2
    Manvendra K Singh
    Institut fur Molekularbiologie, Medizinische Hochschule Hannover, 30625 Hannover, Germany
    Development 132:2697-707. 2005
    ..2004a; Harrelson et al., 2004), Tbx20 may ensure progression to a multi-chambered heart by repressing Tbx2 in the myocardial precursor cells of the linear heart tube destined to form the chambers...
  29. pmc Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the atrioventricular canal: implications for cardiac chamber formation
    Petra E M H Habets
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Genes Dev 16:1234-46. 2002
    ..5 on the ANF TBE-NKE, and was able to repress ANF promoter activity. Our data provide a potential mechanism for chamber-restricted gene activity in which the cooperative action of Tbx2 and Nkx2.5 inhibits expression in the AVC...
  30. ncbi request reprint Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken
    Arjan C Houweling
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Anat Rec 266:93-102. 2002
    ..We conclude that differentiation of chamber myocardium takes place already at HH9 at the ventral side of the linear heart tube, possibly preceded by latero-medial signals in the unfused cardiac sheets...
  31. pmc Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atria
    Willem M H Hoogaars
    Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    Genes Dev 21:1098-112. 2007
    ..These data identify a Tbx3-dependent pathway for the specification and formation of the sinoatrial node, and show that Tbx3 regulates the pacemaker gene expression program and phenotype...
  32. ncbi request reprint Development of the cardiac conduction system: a matter of chamber development
    Antoon F M Moorman
    Molecular and Experimental Cardiology Group, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, Amsterdam, 1105 AZ, The Netherlands
    Novartis Found Symp 250:25-34; discussion 34-43, 276-9. 2003
    ..The possibility that during evolution and ontogenesis of the heat, this development is achieved by localized growth of cardiac chambers rather than of a conduction system is discussed here...
  33. ncbi request reprint Molecular pathway for the localized formation of the sinoatrial node
    Mathilda T M Mommersteeg
    Center for Heart Failure Research, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Circ Res 100:354-62. 2007
    ....
  34. ncbi request reprint Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium
    Arnoud C Fijnvandraat
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Mol Cell Cardiol 35:1461-72. 2003
    ....
  35. ncbi request reprint Vasopressin-induced vasoconstriction is dependent on MAPKerk1/2 phosphorylation
    Jörn O Streefkerk
    The Department of Pharmacotherapy, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    Fundam Clin Pharmacol 18:45-50. 2004
    ..This phosphorylation could be dose dependently prevented by both PD 98059 (100 microm) and U 0126 (10 and 100 microm). These results indicate that vasoconstriction induced by vasopressin is partly regulated by the MAPKerk1/2 pathway...
  36. ncbi request reprint T-box transcription factor Tbx2 represses differentiation and formation of the cardiac chambers
    Vincent M Christoffels
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Dev Dyn 229:763-70. 2004
    ..These findings provide the first evidence that Tbx2 is a determinant in the local repression of chamber-specific gene expression and chamber differentiation...
  37. ncbi request reprint The transcriptional repressor Tbx3 delineates the developing central conduction system of the heart
    Willem M H Hoogaars
    Experimental and Molecular Cardiology Group, AMC, University of Amsterdam, Amsterdam, The Netherlands
    Cardiovasc Res 62:489-99. 2004
    ..We studied the developmental expression pattern and functional aspects of the T-box transcription factor Tbx3, a novel marker for the murine central conduction system (CCS)...
  38. ncbi request reprint Development of the building plan of the heart
    Antoon F M Moorman
    Experimental and Molecular Cardiology Group, Department of Anatomy and Embryology, Academic Medical Centre, Amsterdam, The Netherlands
    Ann N Y Acad Sci 1015:171-81. 2004
    ..With the exception of the outflow tract that becomes incorporated into the ventricles, these regions contribute to the cardiac conduction system...
  39. ncbi request reprint Lineage and morphogenetic analysis of the cardiac valves
    Frederik J de Lange
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Circ Res 95:645-54. 2004
    ..The tricuspid septal leaflet then delaminates from the muscular ventricular septum late in development...
  40. pmc Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system
    Roberto Fiore
    Abteilung Molekularbiologie, Institut fur Allgemeine Zoologie und Genetik, Westfalische Wilhelms Universitat Munster, Schlossplatz 5, D 48149 Munster, Germany
    Mol Cell Biol 25:2310-9. 2005
    ..Our results represent the first genetic analysis of the function of a class 5 semaphorin during embryonic development and identify a role of Sema5A in the regional patterning of the vasculature...
  41. ncbi request reprint Comparative analysis of the natriuretic peptide precursor gene cluster in vertebrates reveals loss of ANF and retention of CNP-3 in chicken
    Arjan C Houweling
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Dev Dyn 233:1076-82. 2005
    ..These results show that, in chicken, the Nppa gene has been lost from the natriuretic peptide precursor gene cluster, whereas the CNP-3 gene has been retained...
  42. ncbi request reprint Cardiac expression of Gal4 causes cardiomyopathy in a dose-dependent manner
    Petra E M H Habets
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Muscle Res Cell Motil 24:205-9. 2003
    ..Our results indicate that expression of a transgene in the heart may non-specifically cause HF in a dose-dependent manner...
  43. ncbi request reprint Expression and regulation of the atrial natriuretic factor encoding gene Nppa during development and disease
    Arjan C Houweling
    Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    Cardiovasc Res 67:583-93. 2005
    ..Here we review current knowledge on the transcriptional regulation of the Nppa gene...
  44. ncbi request reprint Left and right ventricular contributions to the formation of the interventricular septum in the mouse heart
    Diego Franco
    Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaen, 23071 Jaen, Spain
    Dev Biol 294:366-75. 2006
    ..Examination of the orientation as well as the distribution of labeled cells in clusters provides new insights into the morphogenesis of the septum...
  45. ncbi request reprint Cardiac chamber formation: development, genes, and evolution
    Antoon F M Moorman
    Department of Anatomy and Embryology, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    Physiol Rev 83:1223-67. 2003
    ..This view on the development of cardiac design unfolds fascinating possibilities for future research...
  46. ncbi request reprint Two distinct pools of mesenchyme contribute to the development of the atrial septum
    Mathilda T M Mommersteeg
    Molecular and Experimental Cardiology Group, Academic Medical Centre, University of Amsterdam, The Netherlands
    Circ Res 99:351-3. 2006
    ..From the stance of morphological description, therefore, there is little thus far to substantiate the existence of an atrial spine...
  47. ncbi request reprint Architectural plan for the heart: early patterning and delineation of the chambers and the nodes
    Vincent M Christoffels
    Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Trends Cardiovasc Med 14:301-7. 2004
    ....
  48. ncbi request reprint Anatomic substrates for cardiac conduction
    Antoon F M Moorman
    Department of Anatomy and Embryology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
    Heart Rhythm 2:875-86. 2005
  49. doi request reprint Distinct regulation of developmental and heart disease-induced atrial natriuretic factor expression by two separate distal sequences
    Thomas Horsthuis
    Heart Failure Research Center, AMC, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    Circ Res 102:849-59. 2008
    ..These results show that distinct distal regulatory sequences and divergent regulatory pathways control fetal ventricular activity and reactivation of Nppa during cardiac disease, respectively...