Research Topics
Genomes and Genes
| V M ChristoffelsSummaryAffiliation: Academic Medical Center Country: The Netherlands Publications
| Collaborators
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Detail Information
Publications
Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heartV M Christoffels
Experimental and Molecular Cardiology Group, University of Amsterdam, The Netherlands
Dev Biol 224:263-74. 2000....
Tbx2 and Tbx3 induce atrioventricular myocardial development and endocardial cushion formationReena Singh
Institut fur Molekularbiologie, Medizinische Hochschule Hannover, Germany
Cell Mol Life Sci 69:1377-89. 2012..Tbx2 and Tbx3 trigger development of the cushions through a regulatory feed-forward loop with Bmp2, thus providing a mechanism for the co-localization and coordination of these important processes in heart development...- T-box factors determine cardiac designW M H Hoogaars
Heart Failure Research Center, Department of Anatomy and Embryology, Academic Medical Center, Amsterdam, The Netherlands
Cell Mol Life Sci 64:646-60. 2007..Insight into the roles of T-box factors in these processes will enhance our understanding of heart formation and the underlying molecular regulatory pathways... - Formation of the venous pole of the heart from an Nkx2-5-negative precursor population requires Tbx18Vincent M Christoffels
Department of Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
Circ Res 98:1555-63. 2006.... 
Development of the pacemaker tissues of the heartVincent M Christoffels
Heart Failure Research Center, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Circ Res 106:240-54. 2010..It will also shed light on the etiology of inborn and acquired errors of nodal tissues...- Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocolA F Moorman
Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
J Histochem Cytochem 49:1-8. 2001..The radioactive ISH procedure scarcely permitted detection of these sequences, underscoring the value of this novel method... - Gene and cluster-specific expression of the Iroquois family members during mouse developmentA C Houweling
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
Mech Dev 107:169-74. 2001..The results indicate that the Irx genes are coordinately regulated at the level of the cluster... - Presence of functional sarcoplasmic reticulum in the developing heart and its confinement to chamber myocardiumA F Moorman
Experimental and Molecular Cardiology Group, Cardiovascular Research Institute Amsterdam, Academic Medical Center, Amsterdam, 1105 AZ, The Netherlands
Dev Biol 223:279-90. 2000..iii) The pattern of SERCA2a expression underscores a manner of chamber development by differentiation at the outer curvature, rather than by segmentation of the linear heart tube... - The cardiac sodium channel displays differential distribution in the conduction system and transmural heterogeneity in the murine ventricular myocardiumC A Remme
Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Basic Res Cardiol 104:511-22. 2009..This differential distribution of the cardiac sodium channel may have profound consequences for conduction disease phenotypes and arrhythmogenesis in the setting of sodium channel disease... - Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv miceM Campione
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Dev Biol 231:252-64. 2001..Furthermore, altered Pitx2 expression correlated with the occurrence of DORV. Our data reveal the existence of molecular isomerism not only in the atrial, but also in the ventricular compartment of the heart... - Sox4 mediates Tbx3 transcriptional regulation of the gap junction protein Cx43C J J Boogerd
Heart Failure Research Centre, Academic Medical Centre, University of Amsterdam, The Netherlands
Cell Mol Life Sci 68:3949-61. 2011..Mechanistically, we postulate that Sox4 is a mediator of Tbx3 transcriptional activity... - Chamber formation and morphogenesis in the developing mammalian heartV M Christoffels
Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, Amsterdam, 1105, The Netherlands
Dev Biol 223:266-78. 2000.... - A single regulatory module of the carbamoylphosphate synthetase I gene executes its hepatic program of expressionV M Christoffels
Department of Anatomy and Embryology and the Genetically Modified Mice Facility, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
J Biol Chem 275:40020-7. 2000.... - Effect of amiodarone and dronedarone administration in rats on thyroid hormone-dependent gene expression in different cardiac componentsI Stoykov
Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, The Netherlands
Eur J Endocrinol 156:695-702. 2007..In view of their different actions on thyroid hormone receptor (TR) isoforms we set out to investigate whether amiodarone (AM) and dronedarone (Dron) have different and/or component-specific effects on cardiac gene expression... - Acquisition of high quality DNA for massive parallel sequencing by in vivo chromatin immunoprecipitationM van den Boogaard
Heart Failure Research Centre, Academic Medical Centre, section K2, Amsterdam, The Netherlands
Methods Mol Biol 977:53-64. 2013..Although the method makes use of adult mouse hearts, it can, with relative ease, be adapted for the in vivo ChIP isolation of DNA from other cell and tissue sources with the intention of massive parallel sequencing... - Nuclear recruitment assay as a tool to validate transcription factor interactions in Mammalian cellsC J J Boogerd
Heart Failure Research Center, Academic Medical Center, section K2, Amsterdam, The Netherlands
Methods Mol Biol 977:243-8. 2013..We provide a detailed protocol for an intracellular immunofluorescence technique that may be used as an alternative or complimentary study for transcription factor interaction verification... - A molecular and genetic outline of cardiac morphogenesisM S Rana
Heart Failure Research Center, Department of Anatomy, Embryology and Physiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Acta Physiol (Oxf) 207:588-615. 2013..In this review, we provide an overview of the building plan of the vertebrate four-chambered heart, with a special focus on cardiac progenitor cell specification, differentiation and deployment during arterial pole development... - Vasopressin-induced vasoconstriction is dependent on MAPKerk1/2 phosphorylationJörn O Streefkerk
The Department of Pharmacotherapy, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Fundam Clin Pharmacol 18:45-50. 2004..This phosphorylation could be dose dependently prevented by both PD 98059 (100 microm) and U 0126 (10 and 100 microm). These results indicate that vasoconstriction induced by vasopressin is partly regulated by the MAPKerk1/2 pathway... - Cardiac chamber formation: development, genes, and evolutionAntoon F M Moorman
Department of Anatomy and Embryology, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Physiol Rev 83:1223-67. 2003..This view on the development of cardiac design unfolds fascinating possibilities for future research... - The heart-forming fields: one or multiple?Antoon F M Moorman
Heart Failure Research Center, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
Philos Trans R Soc Lond B Biol Sci 362:1257-65. 2007.... - Development of the cardiac conduction system: a matter of chamber developmentAntoon F M Moorman
Molecular and Experimental Cardiology Group, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, Amsterdam, 1105 AZ, The Netherlands
Novartis Found Symp 250:25-34; discussion 34-43, 276-9. 2003..The possibility that during evolution and ontogenesis of the heat, this development is achieved by localized growth of cardiac chambers rather than of a conduction system is discussed here... - Msx1 and Msx2 are functional interacting partners of T-box factors in the regulation of Connexin43Kees Jan Boogerd
Department of Anatomy and Embryology, Heart Failure Research Centre, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands
Cardiovasc Res 78:485-93. 2008..Discovering protein partners of Tbx2 and Tbx3 will shed light on the mechanisms by which these factors regulate these gene programs... - TBX3 and its splice variant TBX3 + exon 2a are functionally similarWillem M H Hoogaars
Center for Heart Failure Research, Academic Medical Centre, Amsterdam, The Netherlands
Pigment Cell Melanoma Res 21:379-87. 2008..The results suggest that in the assays used, Tbx3 and Tbx3 + 2a are functionally equivalent and that like Tbx2, Tbx3 may also function as an anti-senescence factor in melanoma... - A gain-of-function TBX5 mutation is associated with atypical Holt-Oram syndrome and paroxysmal atrial fibrillationAlex V Postma
Heart Failure Research Center, L2 108 1, Academic Medical Center, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
Circ Res 102:1433-42. 2008.... - Transcription factor Tbx3 is required for the specification of the atrioventricular conduction systemMartijn L Bakker
Heart Failure Research Center, Academic Medical Center, Amsterdam, The Netherlands
Circ Res 102:1340-9. 2008..Our data suggest a mechanism in which Tbx3 represses differentiation into ventricular working myocardium, thereby imposing the conduction system phenotype on cells within its expression domain... - Tbx20 is essential for cardiac chamber differentiation and repression of Tbx2Manvendra K Singh
, Medizinische Hochschule Hannover, 30625 Hannover, Germany
Development 132:2697-707. 2005..2004a; Harrelson et al., 2004), Tbx20 may ensure progression to a multi-chambered heart by repressing Tbx2 in the myocardial precursor cells of the linear heart tube destined to form the chambers... - Cardiomyocytes derived from embryonic stem cells resemble cardiomyocytes of the embryonic heart tubeArnoud C Fijnvandraat
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
Cardiovasc Res 58:399-409. 2003.... - Regulatory modules in the developing heartPetra E M H Habets
Experimental and Molecular Cardiology Group, Department of Anatomy and Embryology, Academic Medical Center L2-255, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
Cardiovasc Res 58:246-63. 2003..Most of the regulatory modules show a domain of activity broader than the morphological boundary of a cardiac compartment and seem to respond to a patterning program along the antero-posterior axis... - Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the atrioventricular canal: implications for cardiac chamber formationPetra E M H Habets
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Genes Dev 16:1234-46. 2002..5 on the ANF TBE-NKE, and was able to repress ANF promoter activity. Our data provide a potential mechanism for chamber-restricted gene activity in which the cooperative action of Tbx2 and Nkx2.5 inhibits expression in the AVC... - Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chickenArjan C Houweling
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Anat Rec 266:93-102. 2002..We conclude that differentiation of chamber myocardium takes place already at HH9 at the ventral side of the linear heart tube, possibly preceded by latero-medial signals in the unfused cardiac sheets... - Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atriaWillem M H Hoogaars
Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
Genes Dev 21:1098-112. 2007..These data identify a Tbx3-dependent pathway for the specification and formation of the sinoatrial node, and show that Tbx3 regulates the pacemaker gene expression program and phenotype... - Cardiac expression of Gal4 causes cardiomyopathy in a dose-dependent mannerPetra E M H Habets
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
J Muscle Res Cell Motil 24:205-9. 2003..Our results indicate that expression of a transgene in the heart may non-specifically cause HF in a dose-dependent manner... - Molecular pathway for the localized formation of the sinoatrial nodeMathilda T M Mommersteeg
Center for Heart Failure Research, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
Circ Res 100:354-62. 2007.... - T-box transcription factor Tbx2 represses differentiation and formation of the cardiac chambersVincent M Christoffels
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Dev Dyn 229:763-70. 2004..These findings provide the first evidence that Tbx2 is a determinant in the local repression of chamber-specific gene expression and chamber differentiation... - The transcriptional repressor Tbx3 delineates the developing central conduction system of the heartWillem M H Hoogaars
Experimental and Molecular Cardiology Group, AMC, University of Amsterdam, Amsterdam, The Netherlands
Cardiovasc Res 62:489-99. 2004..We studied the developmental expression pattern and functional aspects of the T-box transcription factor Tbx3, a novel marker for the murine central conduction system (CCS)... - Development of the building plan of the heartAntoon F M Moorman
Experimental and Molecular Cardiology Group, Department of Anatomy and Embryology, Academic Medical Centre, Amsterdam, The Netherlands
Ann N Y Acad Sci 1015:171-81. 2004..With the exception of the outflow tract that becomes incorporated into the ventricles, these regions contribute to the cardiac conduction system... - Lineage and morphogenetic analysis of the cardiac valvesFrederik J de Lange
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Circ Res 95:645-54. 2004..The tricuspid septal leaflet then delaminates from the muscular ventricular septum late in development... - Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular systemRoberto Fiore
Abteilung Molekularbiologie, Institut fur Allgemeine Zoologie und Genetik, Westfalische Wilhelms Universitat Munster, Schlossplatz 5, D 48149 Munster, Germany
Mol Cell Biol 25:2310-9. 2005..Our results represent the first genetic analysis of the function of a class 5 semaphorin during embryonic development and identify a role of Sema5A in the regional patterning of the vasculature... - Comparative analysis of the natriuretic peptide precursor gene cluster in vertebrates reveals loss of ANF and retention of CNP-3 in chickenArjan C Houweling
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Dev Dyn 233:1076-82. 2005..These results show that, in chicken, the Nppa gene has been lost from the natriuretic peptide precursor gene cluster, whereas the CNP-3 gene has been retained... - Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardiumArnoud C Fijnvandraat
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
J Mol Cell Cardiol 35:1461-72. 2003.... - Expression and regulation of the atrial natriuretic factor encoding gene Nppa during development and diseaseArjan C Houweling
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Cardiovasc Res 67:583-93. 2005..Here we review current knowledge on the transcriptional regulation of the Nppa gene... - Left and right ventricular contributions to the formation of the interventricular septum in the mouse heartDiego Franco
Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaen, 23071 Jaen, Spain
Dev Biol 294:366-75. 2006..Examination of the orientation as well as the distribution of labeled cells in clusters provides new insights into the morphogenesis of the septum... - TBX5 overexpression stimulates differentiation of chamber myocardium in P19C16 embryonic carcinoma cellsArnoud C Fijnvandraat
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
J Muscle Res Cell Motil 24:211-8. 2003..This indicates that Tbx5 enhances cardiac maturation within this system rather than cardiogenesis... - Two distinct pools of mesenchyme contribute to the development of the atrial septumMathilda T M Mommersteeg
Molecular and Experimental Cardiology Group, Academic Medical Centre, University of Amsterdam, The Netherlands
Circ Res 99:351-3. 2006..From the stance of morphological description, therefore, there is little thus far to substantiate the existence of an atrial spine... - Pitx2c and Nkx2-5 are required for the formation and identity of the pulmonary myocardiumMathilda T M Mommersteeg
Heart Failure Research Center, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
Circ Res 101:902-9. 2007..In conclusion, our data suggest a cellular mechanism for pulmonary myocardium formation and highlight the key roles played by Pitx2c and Nkx2-5 in its formation and identity... - Anatomic substrates for cardiac conductionAntoon F M Moorman
Department of Anatomy and Embryology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
Heart Rhythm 2:875-86. 2005 - Architectural plan for the heart: early patterning and delineation of the chambers and the nodesVincent M Christoffels
Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Trends Cardiovasc Med 14:301-7. 2004.... - Distinct regulation of developmental and heart disease-induced atrial natriuretic factor expression by two separate distal sequencesThomas Horsthuis
Heart Failure Research Center, AMC, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Circ Res 102:849-59. 2008..These results show that distinct distal regulatory sequences and divergent regulatory pathways control fetal ventricular activity and reactivation of Nppa during cardiac disease, respectively...