Research Topics
| Apiradee HongsthongSummaryAffiliation: National Center for Genetic Engineering and Biotechnology Country: Thailand Publications
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Detail Information
Publications
Mutation study of conserved amino acid residues of Spirulina delta 6-acyl-lipid desaturase showing involvement of histidine 313 in the regioselectivity of the enzymeApiradee Hongsthong
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology, King Mongkut s University of Technology Thonburi Bangkhuntien, Bangkhuntien, 10150 Bangkok, Thailand
Appl Microbiol Biotechnol 66:74-84. 2004....- The expression of three desaturase genes of Spirulina platensis in Escherichia coli DH5alpha. Heterologous expression of Spirulina-desaturase genesHongsthong Apiradee
BEC Unit, National Center for Genetic Engineering and Biotechnology, 83 Moo8, Thakham, Bangkhuntien, Bangkok 10150, Thailand
Mol Biol Rep 31:177-89. 2004..This suggests that while this enzyme is expressed in the heterologous host in its active form, its function in vivo is suppressed, as the electron carriers of the host system cannot complement the system of Spirulina platensis... - Revealing the complementation of ferredoxin by cytochrome b (5) in the Spirulina- (6)-desaturation reaction by N-terminal fusion and co-expression of the fungal-cytochrome b (5) domain and Spirulina- (6)-acyl-lipid desaturaseApiradee Hongsthong
National Center for Genetic Engineering and Biotechnology, 83 Moo8, Thakham, Bangkhuntien, Bangkok, 10150, Thailand
Appl Microbiol Biotechnol 72:1192-201. 2006..Furthermore, the free form of cytochrome b (5) domain can also enhance GLA production by the Spirulina-desD gene in yeast cells... - Revealing differentially expressed proteins in two morphological forms of Spirulina platensis by proteomic analysisApiradee Hongsthong
National Center for Genetic Engineering and Biotechnology, 83 Moo8, Thakham, Bangkhuntien, Bangkok 10150, Thailand
Mol Biotechnol 36:123-30. 2007..Thus, this study is the first to show evidence at the protein level that may explain this morphological transformation in Spirulina... 
Comparative analysis of the Spirulina platensis subcellular proteome in response to low- and high-temperature stresses: uncovering cross-talk of signaling componentsPavinee Kurdrid
BEC Unit, National Center for Genetic Engineering and Biotechnology, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand
Proteome Sci 9:39. 2011....
Truncation mutants highlight a critical role for the N- and C-termini of the Spirulina Delta(6) desaturase in determining regioselectivityPavinee Kurdrid
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology, King Mongkut s University of Technology Thonburi Bangkhuntien, 83 Moo 8, Thakham, Bangkhuntien, Bangkok, 10150, Thailand
Mol Biotechnol 38:203-9. 2008....- A combined stress response analysis of Spirulina platensis in terms of global differentially expressed proteins, and mRNA levels and stability of fatty acid biosynthesis genesWattana Jeamton
Pilot Plant Development and Training Institute, King Mongkut s University of Technology, Thonburi, Bangkok, Thailand
FEMS Microbiol Lett 281:121-31. 2008..The data demonstrate that when the cells encounter cold stress with energy limitation, they can maintain their homeoviscous adaptation ability via mRNA stability... - Functional expression of Spirulina-Delta6 desaturase gene in yeast, Saccharomyces cerevisiaePavinee Kurdrid
Pilot Plant Training and Development Institute, King Mongkut's University of Technology-Thonburi, 83 Moo8, Thakham, Bangkhuntien, Bangkok 10150, Thailand
Mol Biol Rep 32:215-26. 2005..coli. This means that the enzyme expressed in S. cerevisiae can catalyze the biosynthesis of the product, GLA, in vivo... - Effect of two intermediate electron donors, NADPH and FADH(2), on Spirulina Delta (6)-desaturase co-expressed with two different immediate electron donors, cytochrome b (5) and ferredoxin, in Escherichia coliPavinee Kurdrid
Pilot Plant Development and Training Institute, King Mongkut s University of Technology Thonburi Bangkhuntien, Bangkok 10150, Thailand
Mol Biol Rep 34:261-6. 2007..Therefore, if the pools of the immediate and intermediate electron donors in the cells are manipulated, the GLA production in the heterologous host will be affected... - Identification of regulatory regions and regulatory protein complexes of the Spirulina desD gene under temperature stress conditions: role of thioredoxin as an inactivator of a transcriptional repressor GntR under low-temperature stressPavinee Kurdrid
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology, King Mongkut s University of Technology Thonburi Bangkhuntien, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok 10150, Thailand
Biochem Cell Biol 90:621-35. 2012..Furthermore, Trx was shown to play a critical role as a reducing agent that inactivates the Spirulina desD repressor, GntR. Consequently, the desD gene expression is induced under the low-temperature condition... - Isolation and functional characterization of Spirulina D6D gene promoter: role of a putative GntR transcription factor in transcriptional regulation of D6D gene expressionSanjukta Subudhi
Pilot Plant Development and Training Institute, King Mongkut s University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand
Biochem Biophys Res Commun 365:643-9. 2008..Taken together, our results suggest complex regulation of D6D gene expression in Spirulina... - Identification of a heat shock-responsive cis-acting DNA sequence and its transcriptional regulator: Their roles in the expression of the Spirulina-desD gene in response to heat stressPavinee Kurdrid
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology, King Mongkut s University of Technology Thonburi Bangkhuntien, 83 Moo 8, Thakham, Bangkhuntien, Bangkok 10150, Thailand
J Biosci Bioeng 109:205-10. 2010....