Direk Limmathurotsakul

Summary

Affiliation: Mahidol University
Country: Thailand

Publications

  1. pmc Rapid immunofluorescence microscopy for diagnosis of melioidosis
    Vanaporn Wuthiekanun
    Faculty of Tropical Medicine, Wellcome Trust Oxford University Mahidol University Tropical Medicine Research Program, Bangkok, Thailand
    Clin Diagn Lab Immunol 12:555-6. 2005
  2. pmc Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 7:e2105. 2013
  3. pmc Activities of daily living associated with acquisition of melioidosis in northeast Thailand: a matched case-control study
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 7:e2072. 2013
  4. pmc Fool's gold: Why imperfect reference tests are undermining the evaluation of novel diagnostics: a reevaluation of 5 diagnostic tests for leptospirosis
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Clin Infect Dis 55:322-31. 2012
  5. pmc Melioidosis in animals, Thailand, 2006-2010
    Direk Limmathurotsakul
    Mahidol University, Bangkok, Thailand
    Emerg Infect Dis 18:325-7. 2012
  6. ncbi request reprint Variable presentation of neurological melioidosis in Northeast Thailand
    Direk Limmathurotsakul
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 77:118-20. 2007
  7. pmc Increasing incidence of human melioidosis in Northeast Thailand
    Direk Limmathurotsakul
    Department of Tropical Hygiene and Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 82:1113-7. 2010
  8. pmc Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Appl Environ Microbiol 78:876-7. 2012
  9. pmc A simple scoring system to differentiate between relapse and re-infection in patients with recurrent melioidosis
    Direk Limmathurotsakul
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 2:e327. 2008
  10. ncbi request reprint Patterns of organ involvement in recurrent melioidosis
    Direk Limmathurotsakul
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 81:335-7. 2009

Collaborators

Detail Information

Publications61

  1. pmc Rapid immunofluorescence microscopy for diagnosis of melioidosis
    Vanaporn Wuthiekanun
    Faculty of Tropical Medicine, Wellcome Trust Oxford University Mahidol University Tropical Medicine Research Program, Bangkok, Thailand
    Clin Diagn Lab Immunol 12:555-6. 2005
    ..The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively...
  2. pmc Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 7:e2105. 2013
    ..Our objective was to review the literature on the detection of environmental B. pseudomallei, update the risk map for melioidosis, and propose international consensus guidelines for soil sampling...
  3. pmc Activities of daily living associated with acquisition of melioidosis in northeast Thailand: a matched case-control study
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 7:e2072. 2013
    ..The aim of this study was to provide evidence for inoculation, inhalation and ingestion as routes of infection, and develop preventive guidelines based on this evidence...
  4. pmc Fool's gold: Why imperfect reference tests are undermining the evaluation of novel diagnostics: a reevaluation of 5 diagnostic tests for leptospirosis
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Clin Infect Dis 55:322-31. 2012
    ..We hypothesized that our reference standard was imperfect and used statistical modeling to investigate this hypothesis...
  5. pmc Melioidosis in animals, Thailand, 2006-2010
    Direk Limmathurotsakul
    Mahidol University, Bangkok, Thailand
    Emerg Infect Dis 18:325-7. 2012
    ..The highest incidence was in goats (1.63/100,000/year), followed by incidence in pigs and cattle. The estimated incidence of melioidosis in humans in a given region paralleled that of melioidosis in goats...
  6. ncbi request reprint Variable presentation of neurological melioidosis in Northeast Thailand
    Direk Limmathurotsakul
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 77:118-20. 2007
    ..The appropriate duration and choice of parenteral and oral antimicrobial therapy for neurologic melioidosis are also discussed...
  7. pmc Increasing incidence of human melioidosis in Northeast Thailand
    Direk Limmathurotsakul
    Department of Tropical Hygiene and Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 82:1113-7. 2010
    ..63 per 100,000 people (95% CI = 7.33-10.11), the third most common cause of death from infectious diseases in northeast Thailand after human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) and tuberculosis...
  8. pmc Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Appl Environ Microbiol 78:876-7. 2012
    ..We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving...
  9. pmc A simple scoring system to differentiate between relapse and re-infection in patients with recurrent melioidosis
    Direk Limmathurotsakul
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 2:e327. 2008
    ..Distinguishing between the two is important but requires bacterial genotyping. The aim of this study was to develop a simple scoring system to distinguish re-infection from relapse...
  10. ncbi request reprint Patterns of organ involvement in recurrent melioidosis
    Direk Limmathurotsakul
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 81:335-7. 2009
    ..Evaluation of 140 patients with recurrence showed that similar patterns of disease occurred during the first and second episode, independent of whether this was caused by relapse or re-infection...
  11. pmc Repeat blood culture positive for B. pseudomallei indicates an increased risk of death from melioidosis
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Department of Microbiology and Immunology and Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithee Road, Bangkok, Thailand
    Am J Trop Med Hyg 84:858-61. 2011
    ..However, repeat cultures of respiratory secretions, urine, throat swabs, or pus/surface swabs provide no prognostic information. This finding highlights the need for follow-up blood cultures in patients with melioidosis...
  12. pmc Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Mahidol University, Bangkok, Thailand
    PLoS ONE 5:e12485. 2010
    ....
  13. pmc Burkholderia pseudomallei is spatially distributed in soil in northeast Thailand
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 4:e694. 2010
    ..The aim of this study was to evaluate spatial distribution of B. pseudomallei in soil and consider the implications of this for soil sampling strategies...
  14. ncbi request reprint Risk factors for recurrent melioidosis in northeast Thailand
    Direk Limmathurotsakul
    Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand
    Clin Infect Dis 43:979-86. 2006
    ..A recent study revealed that 25% of patients with recurrence had reinfection rather than a relapse resulting from a failure to cure. The aim of this study was to reevaluate these 2 patient groups to define their individual risk factors...
  15. pmc Enzyme-linked immunosorbent assay for the diagnosis of melioidosis: better than we thought
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Clin Infect Dis 52:1024-8. 2011
    ..The new cutoff was unbiased towards misclassification caused by an imperfect gold standard and resulted in an increase in both sensitivity (from 66.4% to 80.2%) and specificity (82.1% and 95.0%)...
  16. pmc Genetic diversity and microevolution of Burkholderia pseudomallei in the environment
    Narisara Chantratita
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 2:e182. 2008
    ..The soil dwelling Gram-negative pathogen Burkholderia pseudomallei is the cause of melioidosis. The diversity and population structure of this organism in the environment is poorly defined...
  17. ncbi request reprint Two randomized controlled trials of ceftazidime alone versus ceftazidime in combination with trimethoprim-sulfamethoxazole for the treatment of severe melioidosis
    Wirongrong Chierakul
    Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Clin Infect Dis 41:1105-13. 2005
    ..It is not known whether TMP-SMX provides an additional benefit...
  18. pmc Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis
    Narisara Chantratita
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    J Clin Microbiol 46:568-73. 2008
    ..The PCR and LAMP assays evaluated here are not sufficiently sensitive to replace culture in our clinical setting...
  19. pmc Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay
    Narisara Chantratita
    Department of Microbiology and Immunology, Mahidol Oxford Tropical Medicine Research Unit, and Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Department of Clinical Pathology, Sappasithiprasong Hospital, Ubon Ratchathani, Thailand Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, United Kingdom
    Am J Trop Med Hyg 89:971-2. 2013
    ..pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic...
  20. ncbi request reprint Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand
    Allen C Cheng
    Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok, 10400, Thailand
    Trans R Soc Trop Med Hyg 100:64-7. 2006
    ..Low sensitivity and specificity of clinical rules is consistent with the protean manifestations of melioidosis and clinical difficulty in identifying patients with melioidosis...
  21. pmc Molecular detection and speciation of pathogenic Leptospira spp. in blood from patients with culture-negative leptospirosis
    Siriphan Boonsilp
    Mahidol Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand
    BMC Infect Dis 11:338. 2011
    ..Leptospira spp. are fastidious bacteria, and we hypothesized that a false-negative culture result may represent infection with a distinct bacterial subset that fail to grow in standard culture medium...
  22. ncbi request reprint Quantitation of B. Pseudomallei in clinical samples
    Vanaporn Wuthiekanun
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 77:812-3. 2007
    ..1, 1.5 x 10(4), 1.1 x 10(5), and 1.1 x 10(7) CFU/mL in these sample types, respectively. This provides important insights into the likely feasibility of future studies such as expression microarray analysis using clinical material...
  23. pmc Role and significance of quantitative urine cultures in diagnosis of melioidosis
    Direk Limmathurotsakul
    Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    J Clin Microbiol 43:2274-6. 2005
    ..The presence of B. pseudomallei in urine during systemic infection is associated with a poor prognosis...
  24. pmc Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis
    Narisara Chantratita
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Clin Vaccine Immunol 14:110-3. 2007
    ..pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%)...
  25. pmc Simultaneous infection with more than one strain of Burkholderia pseudomallei is uncommon in human melioidosis
    Direk Limmathurotsakul
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    J Clin Microbiol 45:3830-2. 2007
    ..Genotyping of 2,058 bacterial colonies isolated from 215 samples taken from 133 patients demonstrated that mixed infection is uncommon (2/133 cases [1.5%; 95% confidence interval, 0.2 to 5.3%])...
  26. pmc Prevalence of melioidosis in patients with suspected pulmonary tuberculosis and sputum smear negative for acid-fast bacilli in northeast Thailand
    Pornpan Suntornsut
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Department of Medicine, Udon Thani Hospital, Udon Thani, Thailand Department of Medical Technology, Udon Thani Hospital, Udon Thani, Thailand Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom Department of Medicine, Cambridge University, Addenbrooke s Hospital, Cambridge, United Kingdom Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 89:983-5. 2013
    ..5%; 95% confidence interval [CI] 0.5-7.3%). We propose that in melioidosis-endemic areas, residual sputum from AFB testing should be routinely cultured for B. pseudomallei...
  27. pmc Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand
    Piengchan Sonthayanon
    Department of Molecular Tropical Medicine and Genetics, Department of Clinical Tropical Medicine, Mahidol Oxford Tropical Medicine Research Unit, Department of Tropical Hygiene, and Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand WHO FAO OIE Collaborating Centre for Reference and Research on Leptospirosis, Western Pacific Region, Communicable Disease Unit, Queensland Health Forensic and Scientific Services, Brisbane, Queensland, Australia Center for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, United Kingdom
    Am J Trop Med Hyg 89:797-9. 2013
    ..We conclude that dual infection occurs, but that serologic tests may overestimate the frequency of co-infections...
  28. ncbi request reprint Trimethoprim/sulfamethoxazole resistance in clinical isolates of Burkholderia pseudomallei
    Vanaporn Wuthiekanun
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    J Antimicrob Chemother 55:1029-31. 2005
    ..Antimicrobial susceptibility testing using the disc diffusion method is commonly used in melioidosis-endemic areas, but may overestimate resistance to trimethoprim/sulfamethoxazole...
  29. pmc Rapid isolation and susceptibility testing of Leptospira spp. using a new solid medium, LVW agar
    Vanaporn Wuthiekanun
    Mahidol Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand
    Antimicrob Agents Chemother 57:297-302. 2013
    ..The use of LVW agar, which enables rapid growth, isolation of single colonies, and simple antimicrobial susceptibility testing for Leptospira spp., provides an opportunity for new areas of fundamental and applied research...
  30. pmc Survey of antimicrobial resistance in clinical Burkholderia pseudomallei isolates over two decades in Northeast Thailand
    Vanaporn Wuthiekanun
    Mahidol Oxford Tropical Medicine Research Unit, Department of Microbiology and Immunology, Mahidol University, Bangkok, Thailand
    Antimicrob Agents Chemother 55:5388-91. 2011
    ..Resistance to carbapenem drugs was not detected. These findings support the current prescribing recommendations for melioidosis...
  31. pmc Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival
    Narisara Chantratita
    Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    J Proteomics 75:1031-42. 2012
    ..1. These data provide novel insights into proteomic alterations that occur during the complex process of morphotype switching, and lend support to the idea that this is associated with a fitness advantage in vivo...
  32. pmc Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of Burkholderia pseudomallei in clinical specimens
    Sarunporn Tandhavanant
    Department of Microbiology and Immunology, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 89:165-8. 2013
    ..The diagnostic sensitivity and specificity of the Mab-IFA was comparable to the Pab-IFA (48.4% versus 45.3% for sensitivity, and 99.8% versus 98.8% for specificity). We have incorporated the Mab-IFA into our routine practice...
  33. pmc Optimization of culture of Leptospira from humans with leptospirosis
    Vanaporn Wuthiekanun
    Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    J Clin Microbiol 45:1363-5. 2007
    ....
  34. pmc Burkholderia pseudomallei genome plasticity associated with genomic island variation
    Sarinna Tumapa
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    BMC Genomics 9:190. 2008
    ..pseudomallei isolates, little is known about the extent of variation between related strains or their association with disease or environmental survival...
  35. pmc Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand
    Piengchan Sonthayanon
    Department of Clinical Tropical Medicine, Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, 420 6 Rajvithi Road, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 84:614-20. 2011
    ..84.6, 95% CI = 69.5-94.1). The rrs LAMP can provide an admission diagnosis in approximately half of patients with leptospirosis, but its clinical utility is reduced by a lower specificity...
  36. pmc Antibodies from patients with melioidosis recognize Burkholderia mallei but not Burkholderia thailandensis antigens in the indirect hemagglutination assay
    Rachaneeporn Tiyawisutsri
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    J Clin Microbiol 43:4872-4. 2005
    ..pseudomallei, B. mallei, and B. thailandensis antigens. We demonstrate a lack of cross-reactivity between B. pseudomallei and B. thailandensis but marked cross-reactivity between B. pseudomallei and B. mallei...
  37. ncbi request reprint Lack of correlation of Burkholderia pseudomallei quantities in blood, urine, sputum and pus
    Gumphol Wongsuvan
    Faculty of Tropical Medicine, Mahidol University, Bangkok Thailand
    Southeast Asian J Trop Med Public Health 40:781-4. 2009
    ..It is likely that an initial seeding event to extracellular organs is followed by independent growth of B. pseudomallei, and that bacteria in the urine were not passively filtered from the bloodstream...
  38. pmc Leptospira species in floodwater during the 2011 floods in the Bangkok Metropolitan Region, Thailand
    Janjira Thaipadungpanit
    Mahidol Oxford Tropical Medicine Research Unit and Departments of Microbiology and Immunology, Tropical Hygiene, and Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, United Kingdom
    Am J Trop Med Hyg 89:794-6. 2013
    ..This study provides baseline information on environmental Leptospira in Bangkok together with a set of laboratory tests that could be readily deployed in the event of future flooding...
  39. doi request reprint Melioidosis: a clinical overview
    Direk Limmathurotsakul
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Br Med Bull 99:125-39. 2011
    ..Melioidosis, an infection caused by the environmental Gram-negative bacillus Burkholderia pseudomallei, has emerged as an important cause of morbidity and mortality in Southeast Asia and northern Australia...
  40. pmc Prospective observational study of the frequency and features of intra-abdominal abscesses in patients with melioidosis in northeast Thailand
    Rapeephan R Maude
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Rajthevi, Bangkok, Thailand
    Trans R Soc Trop Med Hyg 106:629-31. 2012
    ..The mortality rate at 4 weeks post-discharge was lower in patients who were abscess-positive vs abscess-negative (10%, 8/77 vs 20%, 31/153)...
  41. pmc Diagnostic accuracy of real-time PCR assays targeting 16S rRNA and lipL32 genes for human leptospirosis in Thailand: a case-control study
    Janjira Thaipadungpanit
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS ONE 6:e16236. 2011
    ..Here, we compare the diagnostic sensitivity and specificity of two real-time PCR assays targeting rrs or lipL32 for the diagnosis of leptospirosis in northeast Thailand...
  42. pmc Accuracy of a commercial IgM ELISA for the diagnosis of human leptospirosis in Thailand
    Varunee Desakorn
    Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 86:524-7. 2012
    ..This was 20 Panbio units, which gave a sensitivity and specificity of 76.1% and 82.6%, respectively, on paired sera. We conclude that using either cutoff value, the accuracy of IgM ELISA is limited in our setting...
  43. pmc Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei
    Narisara Chantratita
    Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
    Proc Natl Acad Sci U S A 108:17165-70. 2011
    ..The mechanism described here represents an explanation for ceftazidime treatment failure, and may be a frequent but undetected resistance event...
  44. pmc Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei
    Narisara Chantratita
    Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    J Bacteriol 189:807-17. 2007
    ..These findings have major implications for therapeutics and vaccine development...
  45. pmc Common TLR1 Genetic Variation Is Not Associated with Death from Melioidosis, a Common Cause of Sepsis in Rural Thailand
    Narisara Chantratita
    Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS ONE 9:e83285. 2014
    ..Our findings highlight the need for additional studies of TLR1 and other innate immune genetic modulators of the inflammatory host response and determinants of sepsis in southeast Asian populations. ..
  46. ncbi request reprint Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
    Narisara Chantratita
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 77:814-7. 2007
    ..4 CFU/ml (0.2-13.5 CFU/ml); this may explain the low sensitivity of PCR for this specimen. The PCR assay described here is not sufficiently sensitive to replace culture in our clinical setting...
  47. pmc Melioidosis vaccines: a systematic review and appraisal of the potential to exploit biodefense vaccines for public health purposes
    Sharon J Peacock
    Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 6:e1488. 2012
    ..Here, we discuss target populations for vaccination, consider the cost-benefit of different vaccination strategies and review potential vaccine candidates...
  48. pmc Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro
    Sarunporn Tandhavanant
    Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    BMC Microbiol 10:303. 2010
    ..Isogenic colony morphology types II and III were generated from 5 parental type I B. pseudomallei isolates using nutritional limitation. Survival of types II and III were compared with type I for all assays...
  49. pmc Estimating the true accuracy of diagnostic tests for dengue infection using bayesian latent class models
    Wirichada Pan-Ngum
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS ONE 8:e50765. 2013
    ..We hypothesized that those estimates might be inaccurate if the accuracy of the reference assays is not perfect. Here, we investigated this using statistical modeling...
  50. pmc Burkholderia pseudomallei Is Genetically Diverse in Agricultural Land in Northeast Thailand
    Vanaporn Wuthiekanun
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 3:e496. 2009
    ..pseudomallei exists despite the effects of flooding and the physical and chemical processes associated with farming. These findings form an important baseline for future studies of environmental B. pseudomallei...
  51. pmc Host responses to melioidosis and tuberculosis are both dominated by interferon-mediated signaling
    Gavin C K W Koh
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    PLoS ONE 8:e54961. 2013
    ....
  52. pmc Staphylococcus aureus bacteraemia in a tropical setting: patient outcome and impact of antibiotic resistance
    Emma K Nickerson
    Mahidol Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand
    PLoS ONE 4:e4308. 2009
    ..Most information on invasive Staphylococcus aureus infections comes from temperate countries. There are considerable knowledge gaps in epidemiology, treatment, drug resistance and outcome of invasive S. aureus infection in the tropics...
  53. pmc Using a web-based application to define the accuracy of diagnostic tests when the gold standard is imperfect
    Cherry Lim
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS ONE 8:e79489. 2013
    ..Here, we describe open-access web-based applications that allow non-experts to apply Bayesian LCMs to their own data sets via a user-friendly interface...
  54. pmc Toll-like receptor 2 impairs host defense in gram-negative sepsis caused by Burkholderia pseudomallei (Melioidosis)
    W Joost Wiersinga
    Center for Infection and Immunity Amsterdam, Academic Medical Center, Amsterdam, The Netherlands
    PLoS Med 4:e248. 2007
    ..Melioidosis is a severe infection caused by the gram-negative bacterium, Burkholderia pseudomallei, that is endemic in Southeast Asia. We aimed to characterize the expression and function of TLRs in septic melioidosis...
  55. pmc Consensus guidelines for dosing of amoxicillin-clavulanate in melioidosis
    Allen C Cheng
    Department of Medicine, University of Melbourne, Melbourne, Australia
    Am J Trop Med Hyg 78:208-9. 2008
    ..For eradication therapy for melioidosis, we recommend 20/5 mg/kg orally, three times daily...
  56. pmc Open-label randomized trial of oral trimethoprim-sulfamethoxazole, doxycycline, and chloramphenicol compared with trimethoprim-sulfamethoxazole and doxycycline for maintenance therapy of melioidosis
    Wipada Chaowagul
    Medical Department, Sappasithiprasong Hospital, Ubon Ratchathani, Thailand
    Antimicrob Agents Chemother 49:4020-5. 2005
    ..7-fold increase of relapse or death. A combination of TMP-SMX and doxycycline is as effective as and better tolerated than the conventional four-drug regimen for the oral treatment phase of melioidosis...
  57. ncbi request reprint Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents
    Chris Druar
    Cangene Corporation, Mississauga, ON, Canada
    FEMS Immunol Med Microbiol 52:78-87. 2008
    ..However, these ELISAs had low diagnostic accuracy in endemic regions, possibly due to previous patient exposure to B. pseudomallei...
  58. pmc High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei
    W Joost Wiersinga
    Academic Medical Center, Meibergdreef 9, Room G2 132, 1105 AZ Amsterdam, The Netherlands
    Infect Immun 75:3074-9. 2007
    ..Gene profiling such as was done here provides an excellent tool to obtain insight into the extent of inflammation activation in patients with severe infection...
  59. pmc Strategies to reduce mortality from bacterial sepsis in adults in developing countries
    Allen C Cheng
    Department of Medicine, University of Melbourne, and the Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia
    PLoS Med 5:e175. 2008
  60. ncbi request reprint A randomized controlled trial of granulocyte colony-stimulating factor for the treatment of severe sepsis due to melioidosis in Thailand
    Allen C Cheng
    Menzies School of Health Research, Charles Darwin University, Darwin, Australia
    Clin Infect Dis 45:308-14. 2007
    ..Melioidosis is a tropical infectious disease associated with significant mortality. Most deaths occur early and are caused by fulminant sepsis...
  61. ncbi request reprint Invasive Erysipelothrix rhusiopathiae infection in northeast Thailand
    Weera Mahavanakul
    Medical Department, Sappasithiprasong Hospital, Ubon Ratchathani, Thailand
    Southeast Asian J Trop Med Public Health 38:478-81. 2007
    ..Patients presented with variable clinical manifestations including diffused cutaneous lesions, bacteremia and endocarditis. Erysipelothrix infection may be an emerging infection in immunocompromized individuals in Thailand...