Stuart D Blacksell

Summary

Affiliation: Mahidol University
Country: Thailand

Publications

  1. ncbi request reprint Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR
    Diagn Microbiol Infect Dis 60:43-9. 2008
  2. pmc Comparison of performance of serum and plasma in panbio dengue and Japanese encephalitis virus enzyme-linked immunosorbent assays
    Stuart D Blacksell
    Lao Oxford Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos
    Am J Trop Med Hyg 87:573-5. 2012
  3. pmc Commercial dengue rapid diagnostic tests for point-of-care application: recent evaluations and future needs?
    Stuart D Blacksell
    Center for Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, UK
    J Biomed Biotechnol 2012:151967. 2012
  4. pmc Comparison of seven commercial antigen and antibody enzyme-linked immunosorbent assays for detection of acute dengue infection
    Stuart D Blacksell
    Mahidol University Oxford Tropical Medicine Research Unit MORU, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Clin Vaccine Immunol 19:804-10. 2012
  5. doi request reprint Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand
    Stuart D Blacksell
    Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand
    FEMS Immunol Med Microbiol 52:335-42. 2008
  6. pmc Poor diagnostic accuracy of commercial antibody-based assays for the diagnosis of acute Chikungunya infection
    Stuart D Blacksell
    Mahidol University Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    Clin Vaccine Immunol 18:1773-5. 2011
  7. pmc Accuracy of rapid IgM-based immunochromatographic and immunoblot assays for diagnosis of acute scrub typhus and murine typhus infections in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos
    Am J Trop Med Hyg 83:365-9. 2010
  8. ncbi request reprint Serological and blood culture investigations of Nepalese fever patients
    Stuart D Blacksell
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Trans R Soc Trop Med Hyg 101:686-90. 2007
  9. pmc Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: the need for combining NS1 antigen and IgM/IgG antibody detection to achieve acceptable levels of accuracy
    Stuart D Blacksell
    Mahidol Oxford Tropical Medicine Research Unit MORU, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    Clin Vaccine Immunol 18:2095-101. 2011
  10. ncbi request reprint Comparative susceptibility of indigenous and improved pig breeds to Classical swine fever virus infection: practical and epidemiological implications in a subsistence-based, developing country setting
    S D Blacksell
    CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia
    Trop Anim Health Prod 38:467-74. 2006

Collaborators

Detail Information

Publications46

  1. ncbi request reprint Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR
    Diagn Microbiol Infect Dis 60:43-9. 2008
    ....
  2. pmc Comparison of performance of serum and plasma in panbio dengue and Japanese encephalitis virus enzyme-linked immunosorbent assays
    Stuart D Blacksell
    Lao Oxford Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos
    Am J Trop Med Hyg 87:573-5. 2012
    ..5% (3 of 201 for Dengue/JEV IgM and Dengue IgG) and 2.0% (4 of 201; IgM and NS1) showed discordant pairs. These results demonstrate that plasma containing EDTA is suitable for use in these ELISAs...
  3. pmc Commercial dengue rapid diagnostic tests for point-of-care application: recent evaluations and future needs?
    Stuart D Blacksell
    Center for Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, UK
    J Biomed Biotechnol 2012:151967. 2012
    ..This paper addresses current issues relating to the application of commercial dengue RDTs for the diagnosis of acute dengue virus infection, recent diagnostic evaluations, and identifies future needs...
  4. pmc Comparison of seven commercial antigen and antibody enzyme-linked immunosorbent assays for detection of acute dengue infection
    Stuart D Blacksell
    Mahidol University Oxford Tropical Medicine Research Unit MORU, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Clin Vaccine Immunol 19:804-10. 2012
    ..This study provides strong evidence of the value of combining dengue virus antigen- and antibody-based test results in the ELISA format for the diagnosis of acute dengue infection...
  5. doi request reprint Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand
    Stuart D Blacksell
    Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand
    FEMS Immunol Med Microbiol 52:335-42. 2008
    ..80, Fisher's exact). Within this diversity there were also examples of isolates with identical 56-kDa genotypes that were cultured from patients from the same geographical areas...
  6. pmc Poor diagnostic accuracy of commercial antibody-based assays for the diagnosis of acute Chikungunya infection
    Stuart D Blacksell
    Mahidol University Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    Clin Vaccine Immunol 18:1773-5. 2011
    ..The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection...
  7. pmc Accuracy of rapid IgM-based immunochromatographic and immunoblot assays for diagnosis of acute scrub typhus and murine typhus infections in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos
    Am J Trop Med Hyg 83:365-9. 2010
    ..6% (95% CI = 49.1-60.0%) and 94.1% (95% CI = 92.0-95.7%), respectively. Both assays had relatively good specificity but low sensitivity and thus have limited utility for admission diagnosis...
  8. ncbi request reprint Serological and blood culture investigations of Nepalese fever patients
    Stuart D Blacksell
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Trans R Soc Trop Med Hyg 101:686-90. 2007
    ..Many patients demonstrated static IgM antibody results between paired serum collections, suggesting recent rather than acutely active infections...
  9. pmc Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: the need for combining NS1 antigen and IgM/IgG antibody detection to achieve acceptable levels of accuracy
    Stuart D Blacksell
    Mahidol Oxford Tropical Medicine Research Unit MORU, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    Clin Vaccine Immunol 18:2095-101. 2011
    ..This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue...
  10. ncbi request reprint Comparative susceptibility of indigenous and improved pig breeds to Classical swine fever virus infection: practical and epidemiological implications in a subsistence-based, developing country setting
    S D Blacksell
    CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia
    Trop Anim Health Prod 38:467-74. 2006
    ..This increased longevity probably contributes to the maintenance and spread of disease in a population where generally the contact rate is low...
  11. ncbi request reprint The effect of sample degradation and RNA stabilization on classical swine fever virus RT-PCR and ELISA methods
    Stuart D Blacksell
    CSIRO Australian Animal Health Laboratory, P O Bag 24, Geelong, Victoria 3200, Australia
    J Virol Methods 118:33-7. 2004
    ..Samples stored in glycerol/saline were negative at RT-PCR at day 3 post-exposure although AC-ELISA was still positive at day 14 post-exposure...
  12. pmc Accuracy of AccessBio Immunoglobulin M and Total Antibody Rapid Immunochromatographic Assays for the Diagnosis of Acute Scrub Typhus Infection
    Stuart D Blacksell
    Mahidol University Oxford Tropical Medicine Research Unit MORU, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Rd, Bangkok 10400, Thailand
    Clin Vaccine Immunol 17:263-6. 2010
    ..7 to 97.3%), respectively. For the detection of total antibodies, the sensitivity was 97.6% (122/125 samples; 95% CI, 93.1 to 99.5%), but the specificity was much lower, at 71.4% (75/105 samples; 95% CI, 61.8 to 79.8%)...
  13. pmc Limited diagnostic capacities of two commercial assays for the detection of Leptospira immunoglobulin M antibodies in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR, and Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, UK
    Clin Vaccine Immunol 13:1166-9. 2006
    ..3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira "gold standard" microscopic agglutination test...
  14. ncbi request reprint Prevalence of hepatitis E virus antibodies in pigs: implications for human infections in village-based subsistence pig farming in the Lao PDR
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford University Tropical Medicine Research Collaboration, Mahosot Hospital, Vientiane, Lao PDR
    Trans R Soc Trop Med Hyg 101:305-7. 2007
    ..3% (46/301) amongst village pigs. The age distribution suggested previous in-village HEV pig infections. These findings suggest a zoonotic risk associated with village-based smallholder pig farming...
  15. ncbi request reprint A simple and inexpensive container for the transport of biological specimens in limited resource situations
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford University Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR
    Trans R Soc Trop Med Hyg 100:1084-6. 2006
    ..We have found the PVC transporter to be a useful and cost-efficient durable alternative that meets IATA Packing Instruction 650 biological transport container requirements...
  16. ncbi request reprint The comparative accuracy of 8 commercial rapid immunochromatographic assays for the diagnosis of acute dengue virus infection
    Stuart D Blacksell
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom
    Clin Infect Dis 42:1127-34. 2006
    ..Immunochromatographic tests are rapid diagnostic tests (RDTs) that can be performed at the bedside, but they have not been fully validated for diagnosis of dengue infection...
  17. ncbi request reprint Phylogenetic analysis of the E2 gene of classical swine fever viruses from Lao PDR
    Stuart D Blacksell
    Australian Animal Health Laboratory, CSIRO Livestock Industries, Geelong, Victoria, Australia
    Virus Res 104:87-92. 2004
    ..Alternatively, they have evolved independently of other viruses in the region...
  18. ncbi request reprint Genetic typing of classical swine fever viruses from Lao PDR by analysis of the 5' non-coding region
    Stuart D Blacksell
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Private Bag 24, 3220, Geelong, Victoria, Australia
    Virus Genes 31:349-55. 2005
    ..1 and southern/central isolates falling into subgroup 2.2. These results concur with previous studies of CSF viruses from Lao PDR, although this study recognized the first occurrence of subgroup 2.1 in southern Lao PDR...
  19. pmc Rickettsial infections and fever, Vientiane, Laos
    Simaly Phongmany
    Mahosot Hospital, Vientiane, Laos
    Emerg Infect Dis 12:256-62. 2006
    ..46%, p<0.001). Rickettsioses are an underrecognized cause of undifferentiated febrile illnesses among adults in Laos. This finding has implications for the local empiric treatment of fever...
  20. pmc Prospective study to determine accuracy of rapid serological assays for diagnosis of acute dengue virus infection in Laos
    Stuart D Blacksell
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR
    Clin Vaccine Immunol 14:1458-64. 2007
    ..The findings of this study suggest that currently available RDTs based on the detection of IgM antibodies for the diagnosis of acute dengue virus infections are unlikely to be useful for patient management...
  21. ncbi request reprint Comparison of indirect immunofluorescence assays for diagnosis of scrub typhus and murine typhus using venous blood and finger prick filter paper blood spots
    Rattanaphone Phetsouvanh
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People s Democratic Republic
    Am J Trop Med Hyg 80:837-40. 2009
    ..Because filter paper blood samples do not require sophisticated and expensive storage and transport, they may be an appropriate specimen collection technique for the diagnosis of rickettsial disease in the rural tropics...
  22. pmc Evaluation of the Standard Diagnostics Leptospira IgM ELISA for diagnosis of acute leptospirosis in Lao PDR
    Ampai Tanganuchitcharnchai
    Mahidol Oxford Tropical Medicine Research Unit MORU, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
    Trans R Soc Trop Med Hyg 106:563-6. 2012
    ..However, re-evaluation of the diagnostic cut-off to an OD of 1.7 demonstrated improved diagnostic accuracy overall (sensitivity 70%; specificity 78%)...
  23. pmc The infective causes of hepatitis and jaundice amongst hospitalised patients in Vientiane, Laos
    Bounkong Syhavong
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR
    Trans R Soc Trop Med Hyg 104:475-83. 2010
    ..These patients would be expected to respond to empirical doxycycline therapy which, in the absence of virological diagnosis and treatment, may be an appropriate cost-effective intervention in Lao patients with jaundice/hepatitis...
  24. pmc Diagnostic accuracy of a loop-mediated isothermal PCR assay for detection of Orientia tsutsugamushi during acute Scrub Typhus infection
    Daniel H Paris
    Mahidol Oxford Tropical Medicine Programme, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 5:e1307. 2011
    ..There is an urgent need to develop rapid and accurate point-of-care (POC) technologies for acute scrub typhus diagnosis in low-resource, primary health care settings to guide clinical therapy...
  25. pmc Causes of community-acquired bacteremia and patterns of antimicrobial resistance in Vientiane, Laos
    Rattanaphone Phetsouvanh
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Mahosot Hospital, Vientiane, Lao PDR
    Am J Trop Med Hyg 75:978-85. 2006
    ..4/day, an important exception being B. pseudomallei. Bacteremic infants in this setting require an anti-staphylococcal antibiotic...
  26. pmc Prospective evaluation of commercial antibody-based rapid tests in combination with a loop-mediated isothermal amplification PCR assay for detection of Orientia tsutsugamushi during the acute phase of scrub typhus infection
    Stuart D Blacksell
    Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Clin Vaccine Immunol 19:391-5. 2012
    ..The combination of antibody-based assays with DNA- or antigen-based tests shows promise for improved diagnostic sensitivity...
  27. pmc High rates of homologous recombination in the mite endosymbiont and opportunistic human pathogen Orientia tsutsugamushi
    Piengchan Sonthayanon
    Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 4:e752. 2010
    ....
  28. pmc Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus
    Rattanaphone Phetsouvanh
    Lao Oxford Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People s Democratic Republic
    Am J Trop Med Hyg 88:932-6. 2013
    ....
  29. pmc Orientia tsutsugamushi in human scrub typhus eschars shows tropism for dendritic cells and monocytes rather than endothelium
    Daniel H Paris
    Mahidol Oxford Tropical Medicine Research Programme, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS Negl Trop Dis 6:e1466. 2012
    ..tsutsugamushi from the initial eschar site. This newly described cellular tropism for O. tsutsugamushi may influence its interaction with local host immune responses...
  30. ncbi request reprint Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi
    Rungnapa Luksameetanasan
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 38:91-6. 2007
    ..This study demonstrates that O. tsutsugamushi can often be isolated in vitro from the blood of scrub typhus patients when transported at ambient tropical temperatures for many days...
  31. pmc A prospective assessment of the accuracy of commercial IgM ELISAs in diagnosis of Japanese encephalitis virus infections in patients with suspected central nervous system infections in Laos
    Catrin E Moore
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Mahosot Hospital, Vientiane, Lao PDR
    Am J Trop Med Hyg 87:171-8. 2012
    ..2% (95% CI = 87-100) with Panbio Ravi criteria. Specificities were 89-100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42-100%) and 92.9% (95% CI = 83-98%), respectively...
  32. pmc Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai
    Leonard Izzard
    The Australian Rickettsial Reference Laboratory Barwon Biomedical Research, The Geelong Hospital, Bellarine Street, Geelong, Victoria, Australia
    J Clin Microbiol 48:4404-9. 2010
    ..Therefore, we have proposed the name "Orientia chuto," and the prototype strain of this species is strain Dubai, named after the location in which the patient was infected...
  33. ncbi request reprint A systematic review and meta-analysis of the diagnostic accuracy of rapid immunochromatographic assays for the detection of dengue virus IgM antibodies during acute infection
    Stuart D Blacksell
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, UK
    Trans R Soc Trop Med Hyg 100:775-84. 2006
    ..It is recommended that additional, standardized evaluations are required for other dengue ICTs...
  34. pmc Estimating the true accuracy of diagnostic tests for dengue infection using bayesian latent class models
    Wirichada Pan-Ngum
    Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    PLoS ONE 8:e50765. 2013
    ..We hypothesized that those estimates might be inaccurate if the accuracy of the reference assays is not perfect. Here, we investigated this using statistical modeling...
  35. doi request reprint Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen
    James V Conlan
    CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia
    Trop Anim Health Prod 41:913-20. 2009
    ..The IMB-ELISA has the added advantage that a result can be sensitively and specifically determined by eye, lending it to the possibility of adaptation to a near-to-field test with minimal equipment or expertise needed...
  36. ncbi request reprint Molecular typing of Leptospira spp. based on putative O-antigen polymerase gene (wzy), the benefit over 16S rRNA gene sequence
    Piyada Wangroongsarb
    Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    FEMS Microbiol Lett 271:170-9. 2007
    ....
  37. ncbi request reprint Real-time multiplex PCR assay for detection and differentiation of rickettsiae and orientiae
    Daniel H Paris
    Mahidol Oxford Tropical Medicine Programme, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Trans R Soc Trop Med Hyg 102:186-93. 2008
    ..However, further optimisation and validation on samples taken directly from patients to assess its clinical diagnostic utility is required...
  38. ncbi request reprint Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction
    Piengchan Sonthayanon
    Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 75:1099-102. 2006
    ..PCR using primers to amplify a fragment of the 56-kd gene had a sensitivity and specificity of 29.0% and 99.2%, respectively. PCR has a high specificity but low sensitivity for the rapid diagnosis of scrub typhus in this endemic setting...
  39. doi request reprint Simple, rapid and sensitive detection of Orientia tsutsugamushi by loop-isothermal DNA amplification
    Daniel H Paris
    Mahidol Oxford Tropical Medicine Programme, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithee Road, 10400 Bangkok, Thailand
    Trans R Soc Trop Med Hyg 102:1239-46. 2008
    ..This inexpensive method requires no sophisticated equipment or sample preparation, and may prove useful as a diagnostic assay in financially poor settings; however, it requires further prospective validation in the field setting...
  40. ncbi request reprint Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusion
    Stuart D Blacksell
    Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand
    Clin Infect Dis 44:391-401. 2007
    ..We suggest that the diagnosis of scrub typhus using IFA should be based on a > or =4-fold increase in the titer in paired serum samples and should only be based on a single sample titer when there is an adequate local evidence base...
  41. pmc Diagnosis of scrub typhus
    Gavin C K W Koh
    Faculty of Tropical Medicine, Mahidol University, Bangkok
    Am J Trop Med Hyg 82:368-70. 2010
    ..We discuss the limitations of IFA, debate the value of other methods based on antigen detection and nucleic acid amplification, and outline recommendations for future study...
  42. pmc Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
    Sontana Siritantikorn
    Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Trans R Soc Trop Med Hyg 106:632-5. 2012
    ..984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach...
  43. pmc A Prospective Evaluation of Real-Time PCR Assays for the Detection of Orientia tsutsugamushi and Rickettsia spp. for Early Diagnosis of Rickettsial Infections during the Acute Phase of Undifferentiated Febrile Illness
    Wanitda Watthanaworawit
    Shoklo Malaria Research Unit, Mae Sot, Tak, Thailand Mahidol University Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand Centre for Tropical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom Naval Medical Research Center, Viral and Rickettsial Diseases Department, Silver Spring, Maryland BioFire Diagnostics Incorporated, Salt Lake City, Utah
    Am J Trop Med Hyg 89:308-10. 2013
    ..1%; P = 0.08), although this was not statistically significant. Whole blood and buffy coat, but not serum, were acceptable specimens for these PCRs. Further evaluation of these assays in a larger prospective study is warranted. ..
  44. pmc Predictive diagnostic value of the tourniquet test for the diagnosis of dengue infection in adults
    Mayfong Mayxay
    Wellcome Trust Mahosot Hospital Oxford Tropical Medicine Research Collaboration, Mahosot Hospital, Vientiane, Lao PDR
    Trop Med Int Health 16:127-33. 2011
    ..To examine the accuracy of the admission tourniquet test in the diagnosis of dengue infection among Lao adults...
  45. ncbi request reprint Comparison of outbreaks of H5N1 highly pathogenic avian influenza in wild birds and poultry in Thailand
    Jarunee Siengsanan
    The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand
    J Wildl Dis 45:740-7. 2009
    ..Further understanding of the mechanisms of persistence and ongoing HPAI H5N1 transmission between wild birds and domestic poultry is needed...
  46. ncbi request reprint A randomized comparison of oral chloramphenicol versus ofloxacin in the treatment of uncomplicated typhoid fever in Laos
    Simmaly Phongmany
    Mahosot Hospital, Vientiane, Laos
    Trans R Soc Trop Med Hyg 99:451-8. 2005
    ..Three days ofloxacin was more effective than 14 days chloramphenicol for the in-patient treatment of typhoid fever, irrespective of antibiotic susceptibility, and was of similar cost...