Arora S, Thavaselvam D, Kumar A, Prakash A, Barua A, Sathyaseelan K. Cloning, expression and purification of outer membrane protein (OmpA) of Burkholderia pseudomallei and evaluation of its potential for serodiagnosis of melioidosis. Diagn Microbiol Infect Dis. 2015;81:79-84 pubmed publisher
..The assay showed sensitivity of 82.6% and specificity of 93.75%. The recombinant OmpA based indirect ELISA will be a useful tool for serodiagnosis of melioidosis in large scale rapid screening of clinical samples. ..
Sikarwar B, Singh V, Sharma P, Kumar A, Thavaselvam D, Boopathi M, et al
. DNA-probe-target interaction based detection of Brucella melitensis by using surface plasmon resonance. Biosens Bioelectron. 2017;87:964-969 pubmed publisher
Pathak P, Kumar A, Sarangi P, Bhagyawant S, Thavaselvam D. Cloning, expression and purification of virB10 protein of Brucella melitensis and evaluation of its role as a serological marker for Brucella infection in experimental and natural host. Protein Expr Purif. 2018;145:53-58 pubmed publisher
..Antibody response against VirB10 was detected in experimental and natural host suggesting that it can be explored as potential target for serodiagnosis of Brucella infection. ..
Thavaselvam D, Kumar A, Tiwari S, Mishra M, Prakash A. Cloning and expression of the immunoreactive Brucella melitensis 28 kDa outer-membrane protein (Omp28) encoding gene and evaluation of the potential of Omp28 for clinical diagnosis of brucellosis. J Med Microbiol. 2010;59:421-8 pubmed publisher
..This ELISA system in microtitre plates and a dot-blot format will be useful for the rapid screening of large numbers of samples for the diagnosis of human brucellosis in endemic areas. ..
Pathak P, Kumar A, Thavaselvam D. Evaluation of recombinant porin (rOmp2a) protein as a potential antigen candidate for serodiagnosis of Human Brucellosis. BMC Infect Dis. 2017;17:485 pubmed publisher
..For determination of statistical significance between experimental groups and control groups, Student's t test was performed on the data. Omp2a emerges as a potential antigen candidate for serodiagnosis of human brucellosis. ..