Hsien Hsiung Lee

Summary

Affiliation: Yuan-Shan Research Institute
Country: Taiwan

Publications

  1. ncbi request reprint Structural analysis of the chimeric CYP21P/CYP21 gene in steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Ltd, Yuan Shan Research Institute, Taiwan, Republic of China
    J Hum Genet 47:517-22. 2002
  2. ncbi request reprint Deletion of the C4-CYP21 repeat module leading to the formation of a chimeric CYP21P/CYP21 gene in a 9.3-kb fragment as a cause of steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Ltd, Yuan Shan Research Institute, No 326 Yuan Shan Rd, Sec 2, Yuan Shan, Ilan 264, Taiwan, Republic of China
    Clin Chem 49:319-22. 2003
  3. ncbi request reprint Mutation of IVS2 -12A/C>G in combination with 707-714delGAGACTAC in the CYP21 gene is caused by deletion of the C4-CYP21 repeat module with steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, Ilan 264, Taiwan, Republic of China
    J Clin Endocrinol Metab 88:2726-9. 2003
  4. ncbi request reprint Duplication of 111 bases in exon 1 of the CYP21 gene is combined with deletion of CYP21P-C4B genes in steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, No 326 Yuan Shan Rd, Sec 2, Yuan Shan, Ilan 264, Taiwan, Republic of China
    Mol Genet Metab 79:214-20. 2003
  5. ncbi request reprint The chimeric CYP21P/CYP21 gene and 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, 326 Yuan Shan Road, Sec 2, Yuanshan, 264, Ilan, Taiwan, Republic of China
    J Hum Genet 49:65-72. 2004
  6. ncbi request reprint PCR-based detection of the CYP21 deletion and TNXA/TNXB hybrid in the RCCX module
    Hsien Hsiung Lee
    King Car Food Industrial Co Ltd, Yuan Shan Research Institute, No 326 Yuan Shan Road, Section 2, Yuan Shan, Ilan 264, Taiwan, Republic of China
    Genomics 83:944-50. 2004
  7. ncbi request reprint Use of PCR-based amplification analysis as a substitute for the southern blot method for CYP21 deletion detection in congenital adrenal hyperplasia
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, Taiwan, Republic of China
    Clin Chem 50:1074-6. 2004
  8. ncbi request reprint Diversity of the CYP21P-like gene in CYP21 deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, Taiwan, Republic of China
    DNA Cell Biol 24:1-9. 2005
  9. ncbi request reprint Simultaneous detection of white spot syndrome virus (WSSV) and Taura syndrome virus (TSV) by multiplex reverse transcription-polymerase chain reaction (RT-PCR) in pacific white shrimp Penaeus vannamei
    Jyh Ming Tsai
    King Car Biotechnology Industrial Co, Ltd, Aquaculture Research and Development Center, I Lan, Taiwan, ROC
    Dis Aquat Organ 50:9-12. 2002
  10. doi request reprint An investigation of the C4 gene arrangement in ethnic Chinese (Taiwanese)
    Yung Te Tseng
    Department of Laboratory Medicine, Lotung Poh Ai Hospital, Ilan, Taiwan
    Int J Immunogenet 35:323-9. 2008

Collaborators

  • Fu Sung Lo
  • Fuu Jen Tsai
  • Dau Ming Niu
  • Yung Te Tseng
  • Shu Hua Chang
  • Hsu Hui Wang
  • Jyh Ming Tsai
  • Yann Jinn Lee
  • Shao Yin Chu
  • Pen Jung Wang
  • Jui Hsia Chen
  • Tso Ren Wang
  • Yann Jin Lee
  • Bon chu Chung
  • Du An Wu
  • Ching Yu Lin
  • Lin John Shiau
  • Peter W Y Chan

Detail Information

Publications19

  1. ncbi request reprint Structural analysis of the chimeric CYP21P/CYP21 gene in steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Ltd, Yuan Shan Research Institute, Taiwan, Republic of China
    J Hum Genet 47:517-22. 2002
    ..It is plausible that both consensus sequences are responsible for the gene conversion of these two chimeric genes...
  2. ncbi request reprint Deletion of the C4-CYP21 repeat module leading to the formation of a chimeric CYP21P/CYP21 gene in a 9.3-kb fragment as a cause of steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Ltd, Yuan Shan Research Institute, No 326 Yuan Shan Rd, Sec 2, Yuan Shan, Ilan 264, Taiwan, Republic of China
    Clin Chem 49:319-22. 2003
  3. ncbi request reprint Mutation of IVS2 -12A/C>G in combination with 707-714delGAGACTAC in the CYP21 gene is caused by deletion of the C4-CYP21 repeat module with steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, Ilan 264, Taiwan, Republic of China
    J Clin Endocrinol Metab 88:2726-9. 2003
    ..Surprisingly, this kind of the haplotype of the mutated CYP21 gene has not been reported as a gene deletion...
  4. ncbi request reprint Duplication of 111 bases in exon 1 of the CYP21 gene is combined with deletion of CYP21P-C4B genes in steroid 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, No 326 Yuan Shan Rd, Sec 2, Yuan Shan, Ilan 264, Taiwan, Republic of China
    Mol Genet Metab 79:214-20. 2003
    ..Possibly, the additional 111-base duplicated coding sequence may be generated by multiple intergenic recombinations, while there seems to be no relationship with deletion of the CYP21P-C4B regions...
  5. ncbi request reprint The chimeric CYP21P/CYP21 gene and 21-hydroxylase deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, 326 Yuan Shan Road, Sec 2, Yuanshan, 264, Ilan, Taiwan, Republic of China
    J Hum Genet 49:65-72. 2004
    ....
  6. ncbi request reprint PCR-based detection of the CYP21 deletion and TNXA/TNXB hybrid in the RCCX module
    Hsien Hsiung Lee
    King Car Food Industrial Co Ltd, Yuan Shan Research Institute, No 326 Yuan Shan Road, Section 2, Yuan Shan, Ilan 264, Taiwan, Republic of China
    Genomics 83:944-50. 2004
    ..This rapid, nonradioactive detection method will be beneficial for diagnostic purposes that are limited to the population originally studied...
  7. ncbi request reprint Use of PCR-based amplification analysis as a substitute for the southern blot method for CYP21 deletion detection in congenital adrenal hyperplasia
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, Taiwan, Republic of China
    Clin Chem 50:1074-6. 2004
  8. ncbi request reprint Diversity of the CYP21P-like gene in CYP21 deficiency
    Hsien Hsiung Lee
    King Car Food Industrial Co, Yuan Shan Research Institute, Taiwan, Republic of China
    DNA Cell Biol 24:1-9. 2005
    ..Most of these gene arrangements probably exist in the C4A-XCYP21-TNXB and C4A-CYP21P/CYP21-TNXB gene loci. The existence of the C4A-CYP21P-TNXA/TNXB locus might not be common in CAH patients with 21-hydroxylase deficiency...
  9. ncbi request reprint Simultaneous detection of white spot syndrome virus (WSSV) and Taura syndrome virus (TSV) by multiplex reverse transcription-polymerase chain reaction (RT-PCR) in pacific white shrimp Penaeus vannamei
    Jyh Ming Tsai
    King Car Biotechnology Industrial Co, Ltd, Aquaculture Research and Development Center, I Lan, Taiwan, ROC
    Dis Aquat Organ 50:9-12. 2002
    ..This study is the first report of Penaeus vannamei specimens co-infected with WSSV and TSV being detected using a PCR method via experimental infection...
  10. doi request reprint An investigation of the C4 gene arrangement in ethnic Chinese (Taiwanese)
    Yung Te Tseng
    Department of Laboratory Medicine, Lotung Poh Ai Hospital, Ilan, Taiwan
    Int J Immunogenet 35:323-9. 2008
    ....
  11. ncbi request reprint Chimeric CYP21P/CYP21 and TNXA/TNXB genes in the RCCX module
    Hsien Hsiung Lee
    Department of Medical Research, Mackay Memorial Hospital, No 45, Min Sheng Road, Tanshui, Taipei 251, Taiwan, ROC
    Mol Genet Metab 84:4-8. 2005
    ..In addition, the haplotype of CYP21 with chimera CYP21P/CYP21 causes 21-hydroxylase deficiency in congenital adrenal hyperplasia (CAH), while chimera TNXA/TNXB is associated with Ehlers-Danols syndrome as well as CAH...
  12. ncbi request reprint Molecular identification of combined homozygous and compound heterozygous mutations in the CYP21 gene in simple virilizing congenital adrenal hyperplasia in Taiwan
    Hsu Hui Wang
    Department of Pediatrics, Shin Kong Wu Ho Su Memorial Hospital, No 95, Wen Tsang Road, Taipei, Taiwan
    Acta Paediatr Taiwan 44:339-42. 2003
    ..Further molecular study will focus on the salt-losing type in order to correlate the genotype/phenotype with this study...
  13. ncbi request reprint Novel missense mutations, GCC [Ala306]- > GTC [Val] and ACG [Thr318]- > CCG [Pro], in the CYP11B1 gene cause steroid 11beta-hydroxylase deficiency in the Chinese
    Hsien Hsiung Lee
    Department of Medical Research, Mackay Memorial Hospital, 45 Min Sheng Road, Tanshui, Taipei 251, Taiwan
    Clin Endocrinol (Oxf) 62:418-22. 2005
    ..Steroid 11beta-hydroxylase (CYP11B1) deficiency, an autosomal recessive inherited disease, accounts for 5-8% of congenital adrenal hyperplasia (CAH). It is mainly caused by mutations of nucleotide substitutions in the coding region...
  14. ncbi request reprint Only two amino acid substitutions of I236N and V237E in exon 6 are converted to the CYP21 gene in a Chinese patient with congenital adrenal hyperplasia
    Hsien Hsiung Lee
    Clin Endocrinol (Oxf) 64:227-9. 2006
  15. ncbi request reprint Diversity of the CYP21A2 gene: a 6.2-kb TaqI fragment and a 3.2-kb TaqI fragment mistaken as CYP21A1P
    Hsien Hsiung Lee
    Department of Medical Research, Mackay Memorial Hospital, No 45 Min Sheng Road, Tanshui, Taipei 251, Taiwan
    Mol Genet Metab 88:372-7. 2006
    ..Therefore, the CYP21A2 haplotype not only presents a 3.7-kb TaqI fragment but also may possibly exist in multiple forms including both 6.2- and 3.2-kb fragments...
  16. ncbi request reprint Identification of the size and antigenic determinants of the human C4 gene by a polymerase chain-reaction-based amplification method
    Hsien Hsiung Lee
    Department of Medical Research, Mackay Memorial Hospital, No 45 Min Sheng Road, Tanshui, Taipei Co 251, Taiwan
    Anal Biochem 357:122-7. 2006
    ..This result was consistent with that of the Southern blot analysis. The PCR method is practical for identifying the C4 genotype and can be used to detect other polymorphisms among variants of C4 genes...
  17. ncbi request reprint Low frequency of the CYP21A2 deletion in ethnic Chinese (Taiwanese) patients with 21-hydroxylase deficiency
    Hsien Hsiung Lee
    Department of Medical Research, Mackay Memorial Hospital, 45 Min Sheng Road, Tanshui 251, Taipei County, Taiwan
    Mol Genet Metab 93:450-7. 2008
    ....
  18. ncbi request reprint Use of a PCR-based amplification analysis as a substitute for the Southern blot method to determine the C4A and C4B genes
    Hsien Hsiung Lee
    Department of Medical Research, Mackay Memorial Hospital, No 45 Min Sheng Road, Tanshui, Taipei County 251, Taiwan
    J Immunol Methods 317:126-31. 2006
    ..5 and 49%, respectively in this ethnic Chinese (Taiwanese) cohort. Since no radiolabelling application is involved, the protocol is reliable as a substitute for the Southern blot method for C4A and C4B determination...
  19. ncbi request reprint Congenital adrenal hyperplasia with 11 beta-hydroxylase deficiency
    Shu Hua Chang
    Department of Pediatrics, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
    J Formos Med Assoc 103:860-4. 2004
    ....