Oscar K Lee

Summary

Affiliation: Taipei Veterans General Hospital
Country: Taiwan

Publications

  1. pmc Development of a surface plasmon resonance biosensor for real-time detection of osteogenic differentiation in live mesenchymal stem cells
    Yi Chun Kuo
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 6:e22382. 2011
  2. pmc miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells
    Jui Yu Hsieh
    Department of Life Sciences, Institute of Microbiology and Immunology, National Yang Ming University, Linong St Taipei, Taiwan 11221, Division of Cardiology, Department of Internal Medicine, Tri Service General Hospital, National Defense Medical Center, Chenggong Rd Taipei, Taiwan 11490, Department of Medical Research and Education, Taipei Veteran General Hospital, Shipai Rd, Taipei, Taiwan 11217, Stem Cell Research Center, National Yang Ming University, Linong St Taipei, Taiwan 11221, Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan, Veteran General Hospital Yang Ming Genome Research Center, National Yang Ming University, Linong St Taipei, Taiwan 11221, Department of Education and Research, Taipei City Hospital, Zhengzhou Rd Taipei, Taiwan 10341 and Cancer Research Center and Genome Research Center, National Yang Ming University, Linong St Taipei, Taiwan 11221
    Nucleic Acids Res 41:9753-63. 2013
  3. pmc Human mesenchymal stem cells suppress the stretch-induced inflammatory miR-155 and cytokines in bronchial epithelial cells
    Yi Chun Kuo
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 8:e71342. 2013
  4. pmc In vivo fluorescence imaging reveals the promotion of mammary tumorigenesis by mesenchymal stromal cells
    Chien Chih Ke
    AntiCancer, Inc, San Diego, California, United States of America
    PLoS ONE 8:e69658. 2013
  5. pmc Detection of osteogenic differentiation by differential mineralized matrix production in mesenchymal stromal cells by Raman spectroscopy
    Pei San Hung
    Institute of Biophotonics, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 8:e65438. 2013
  6. ncbi request reprint Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004
  7. doi request reprint Cell contact accelerates replicative senescence of human mesenchymal stem cells independent of telomere shortening and p53 activation: roles of Ras and oxidative stress
    Jennifer H Ho
    Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan
    Cell Transplant 20:1209-20. 2011
  8. pmc CD133-expressing thyroid cancer cells are undifferentiated, radioresistant and survive radioiodide therapy
    Chien Chih Ke
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    Eur J Nucl Med Mol Imaging 40:61-71. 2013
  9. doi request reprint Multiple intravenous transplantations of mesenchymal stem cells effectively restore long-term blood glucose homeostasis by hepatic engraftment and β-cell differentiation in streptozocin-induced diabetic mice
    Jennifer H Ho
    Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan
    Cell Transplant 21:997-1009. 2012
  10. doi request reprint Hypoxia promotes proliferation and osteogenic differentiation potentials of human mesenchymal stem cells
    Shun Pei Hung
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    J Orthop Res 30:260-6. 2012

Collaborators

Detail Information

Publications41

  1. pmc Development of a surface plasmon resonance biosensor for real-time detection of osteogenic differentiation in live mesenchymal stem cells
    Yi Chun Kuo
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 6:e22382. 2011
    ..This SPR biosensor will facilitate future advances in a vast array of fields in biomedical research and medical diagnosis...
  2. pmc miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells
    Jui Yu Hsieh
    Department of Life Sciences, Institute of Microbiology and Immunology, National Yang Ming University, Linong St Taipei, Taiwan 11221, Division of Cardiology, Department of Internal Medicine, Tri Service General Hospital, National Defense Medical Center, Chenggong Rd Taipei, Taiwan 11490, Department of Medical Research and Education, Taipei Veteran General Hospital, Shipai Rd, Taipei, Taiwan 11217, Stem Cell Research Center, National Yang Ming University, Linong St Taipei, Taiwan 11221, Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan, Veteran General Hospital Yang Ming Genome Research Center, National Yang Ming University, Linong St Taipei, Taiwan 11221, Department of Education and Research, Taipei City Hospital, Zhengzhou Rd Taipei, Taiwan 10341 and Cancer Research Center and Genome Research Center, National Yang Ming University, Linong St Taipei, Taiwan 11221
    Nucleic Acids Res 41:9753-63. 2013
    ..These findings suggest that miR-146a-5p is critical to the uncoupling of motility and proliferation of MSCs. Our miRNome data also provide a roadmap for further understanding MSC biology. ..
  3. pmc Human mesenchymal stem cells suppress the stretch-induced inflammatory miR-155 and cytokines in bronchial epithelial cells
    Yi Chun Kuo
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 8:e71342. 2013
    ....
  4. pmc In vivo fluorescence imaging reveals the promotion of mammary tumorigenesis by mesenchymal stromal cells
    Chien Chih Ke
    AntiCancer, Inc, San Diego, California, United States of America
    PLoS ONE 8:e69658. 2013
    ..These findings also suggest the potential risk of enhancing tumor progression in clinical cell therapy using MSCs. Attention has to be paid to patients with high risk of breast cancer when considering cell therapy with MSCs. ..
  5. pmc Detection of osteogenic differentiation by differential mineralized matrix production in mesenchymal stromal cells by Raman spectroscopy
    Pei San Hung
    Institute of Biophotonics, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 8:e65438. 2013
    ..We expect that this study will promote further investigation of stem cell research and clinical applications...
  6. ncbi request reprint Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004
    ..In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow...
  7. doi request reprint Cell contact accelerates replicative senescence of human mesenchymal stem cells independent of telomere shortening and p53 activation: roles of Ras and oxidative stress
    Jennifer H Ho
    Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan
    Cell Transplant 20:1209-20. 2011
    ..The contact-induced senescence model reported in this study will serve as a useful model system that allows further study of the molecular mechanisms of senescence in MSCs...
  8. pmc CD133-expressing thyroid cancer cells are undifferentiated, radioresistant and survive radioiodide therapy
    Chien Chih Ke
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    Eur J Nucl Med Mol Imaging 40:61-71. 2013
    ..The aim of this study was to investigate whether CD133(+) cells contribute to the radioresistance of thyroid cancer and thus potentiate future recurrence and metastasis...
  9. doi request reprint Multiple intravenous transplantations of mesenchymal stem cells effectively restore long-term blood glucose homeostasis by hepatic engraftment and β-cell differentiation in streptozocin-induced diabetic mice
    Jennifer H Ho
    Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan
    Cell Transplant 21:997-1009. 2012
    ..Liver engraftment and differentiation into insulin-producing cells account for the long-term therapeutic effects of MSCs...
  10. doi request reprint Hypoxia promotes proliferation and osteogenic differentiation potentials of human mesenchymal stem cells
    Shun Pei Hung
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    J Orthop Res 30:260-6. 2012
    ..Taken together, hypoxia provides a favorable culture condition to promote proliferation as well as osteogenesis of MSCs through differential growth factor production...
  11. doi request reprint Comparison of potentials between stem cells isolated from human anterior cruciate ligament and bone marrow for ligament tissue engineering
    Ming Te Cheng
    Department of Surgery, National Yang Ming University, Taipei, Taiwan
    Tissue Eng Part A 16:2237-53. 2010
    ....
  12. pmc Stem cell therapy for liver disease: parameters governing the success of using bone marrow mesenchymal stem cells
    Tom K Kuo
    Institute of Biopharmaceutical Science, National Yang Ming University, Taipei, Taiwan
    Gastroenterology 134:2111-21, 2121.e1-3. 2008
    ..The aim of this study was to critically analyze the various parameters governing the success of bone marrow-derived mesenchymal stem cell-based therapy for treatment of liver diseases...
  13. pmc Rapid generation of mature hepatocyte-like cells from human induced pluripotent stem cells by an efficient three-step protocol
    Yu Fan Chen
    Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan
    Hepatology 55:1193-203. 2012
    ..Conclusion: We have established a rapid and efficient differentiation protocol that is able to generate functional hepatocyte-like cells from human iPSCs. This may offer an alternative option for treatment of liver diseases...
  14. doi request reprint Matrix stiffness regulation of integrin-mediated mechanotransduction during osteogenic differentiation of human mesenchymal stem cells
    Yu Ru V Shih
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    J Bone Miner Res 26:730-8. 2011
    ..Taken together, our results provide evidence that the matrix rigidity affects the osteogenic outcome of MSCs through mechanotransduction events that are mediated by α(2)-integrin...
  15. doi request reprint Thymosin beta-4 directs cell fate determination of human mesenchymal stem cells through biophysical effects
    Jennifer H Ho
    Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan
    J Orthop Res 28:131-8. 2010
    ..Such findings suggest that Tbeta(4), a ubiquitous peptide, may be involved in osteoporosis when its intracellular concentration is elevated. Further investigation of targeting Tbeta(4) for future osteoporosis treatment is warranted...
  16. doi request reprint Conditioned mesenchymal stem cells attenuate progression of chronic kidney disease through inhibition of epithelial-to-mesenchymal transition and immune modulation
    Jei Wen Chang
    Department of Pediatrics, Taipei Veterans General Hospital, Taipei, Taiwan
    J Cell Mol Med 16:2935-49. 2012
    ..Together, our studies indicated that conditioned MSCs preserve renal function by their anti-fibrotic and anti-inflammatory effects. Transplantation of conditioned MSCs may be useful in treating CKD...
  17. doi request reprint Phosphoproteomic analysis of human mesenchymal stromal cells during osteogenic differentiation
    Ting Lo
    Department of Medical Research and Education and Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan
    J Proteome Res 11:586-98. 2012
    ..The findings in this study may also shed light on the development of new therapeutic targets for metabolic bone diseases such as osteoporosis and osteomalacia...
  18. ncbi request reprint Amelioration of paraquat-induced pulmonary injury by mesenchymal stem cells
    Hsin Lin Tsai
    Institute of Clinical Medicine, School of Medicine, National Yang Ming University, Taipei, Taiwan
    Cell Transplant 22:1667-81. 2013
    ..The results of this preclinical study demonstrate that transplantation of MSCs may be a promising therapy and should be further validated clinically. ..
  19. doi request reprint Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies
    Han Wen Guo
    Institute of Biophotonics, National Yang Ming University, 155 Li Nong Street, Section 2, Taipei 11221, Taiwan
    J Biomed Opt 13:050505. 2008
    ..These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies...
  20. doi request reprint Amine-surface-modified superparamagnetic iron oxide nanoparticles interfere with differentiation of human mesenchymal stem cells
    You Kang Chang
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    J Orthop Res 30:1499-506. 2012
    ....
  21. ncbi request reprint Elevation of protein kinase Cα stimulates osteogenic differentiation of mesenchymal stem cells through the TAT-mediated protein transduction system
    Wei Kai Hua
    a Institute of Biopharmaceutical Sciences, National Yang Ming University, No 155, Sec 2, Linong St, Taipei 112, Taiwan
    Biochem Cell Biol 91:443-8. 2013
    ..This may promote the application of PKCs in therapeutic treatment. ..
  22. doi request reprint Hypoxia-induced secretion of TGF-β1 in mesenchymal stem cell promotes breast cancer cell progression
    Shun Pei Hung
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    Cell Transplant 22:1869-82. 2013
    ..Therefore, extra caution has to be exercised when considering hypoxia pretreatment of MSCs before cell transplantation into patients for therapeutic purposes, particularly in patients susceptible to tumor growth. ..
  23. ncbi request reprint Growth of mesenchymal stem cells on electrospun type I collagen nanofibers
    Yu Ru V Shih
    Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan
    Stem Cells 24:2391-7. 2006
    ..Further efforts are necessary to enhance their biomimetic properties...
  24. doi request reprint An iTRAQ proteomic study reveals an association between diet-induced enhanced fatty acid metabolism and the development of glucose intolerance in prediabetic mice
    Jennifer H Ho
    Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan
    J Proteome Res 12:1120-33. 2013
    ....
  25. doi request reprint Regulation of the fate of human mesenchymal stem cells by mechanical and stereo-topographical cues provided by silicon nanowires
    Shu Wen Kuo
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    Biomaterials 33:5013-22. 2012
    ..The findings in this study provide insights in terms of the design of stereo-topographical structures for use in tissue engineering, bone regeneration and relevant medical applications...
  26. doi request reprint Coordinated changes of mitochondrial biogenesis and antioxidant enzymes during osteogenic differentiation of human mesenchymal stem cells
    Chien Tsun Chen
    Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, Taiwan
    Stem Cells 26:960-8. 2008
    ..Together, our findings suggest that coordinated regulation of mitochondrial biogenesis and antioxidant enzymes occurs synergistically during osteogenic differentiation of hMSCs...
  27. doi request reprint Restoration of bone mass and strength in glucocorticoid-treated mice by systemic transplantation of CXCR4 and cbfa-1 co-expressing mesenchymal stem cells
    Chun Yang Lien
    Institute of Biopharmaceutical Science, National Yang Ming University, Taipei, Taiwan, ROC
    J Bone Miner Res 24:837-48. 2009
    ..To our knowledge, this is the first report to show unequivocally the feasibility of ameliorating glucocorticoid-induced osteoporosis by systemic transplantation of genetically manipulated MSCs...
  28. doi request reprint Therapeutic effects of umbilical cord blood-derived mesenchymal stem cell transplantation in experimental lupus nephritis
    Jei Wen Chang
    Division of Immunology and Nephrology, Department of Pediatrics, Taipei Veterans General Hospital, Taipei, Taiwan
    Cell Transplant 20:245-57. 2011
    ..Therapeutic effects demonstrated in this preclinical study support further exploration of the possibility to use uMSCs from mismatched donors in lupus nephritis treatment...
  29. doi request reprint Glucose reduction prevents replicative senescence and increases mitochondrial respiration in human mesenchymal stem cells
    Ting Lo
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    Cell Transplant 20:813-25. 2011
    ..Taken together, our findings suggest that MSCs utilize energy more efficiently under restricted glucose treatment and exhibit greater self-renewal and antisenescence abilities, while their differentiation potentials remain unaffected...
  30. doi request reprint Mesenchymal stem cells negatively regulate dendritic lineage commitment of umbilical-cord-blood-derived hematopoietic stem cells: an unappreciated mechanism as immunomodulators
    Hsiu Yu Lai
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    Tissue Eng Part A 16:2987-97. 2010
    ..Further, HSC-derived DCs in the presence of MSCs are functionally impaired, which could be partly due to the upregulation of IL6 secretion...
  31. doi request reprint Mesenchymal stem cell therapy for nonmusculoskeletal diseases: emerging applications
    Tom K Kuo
    Stem Cell Research Center, National Yang Ming University, Taiwan
    Cell Transplant 18:1013-28. 2009
    ..In this article, we review the recent developments in mesenchymal stem cell research with respect to their emerging properties and applications in nonmusculoskeletal diseases...
  32. pmc Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cells
    You Kang Chang
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    J Biomed Sci 18:54. 2011
    ..The purpose of this study is to investigate whether transplantation of human MSCs (hMSCs) can rescue cerebellar PCs and ameliorate motor function deterioration in SCA in a pre-clinical animal model...
  33. doi request reprint The effects of actin cytoskeleton perturbation on keratin intermediate filament formation in mesenchymal stem/stromal cells
    Tzu Hao Chang
    Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan
    Biomaterials 35:3934-44. 2014
    ..2. Such findings offer new insight in lineage commitment of MSCs and further scaffold design in MSC-based tissue engineering...
  34. ncbi request reprint Optimization of culture conditions for stem cells derived from human anterior cruciate ligament and bone marrow
    Ming Te Cheng
    Department of Surgery, School of Medicine, National Yang Ming University, Taipei, Taiwan
    Cell Transplant 23:791-803. 2014
    ..With increasing understanding of the effects of different culture media and growth factors, manipulation of stem cells in the desired direction for ligament tissue engineering can be achieved. ..
  35. ncbi request reprint Cbfb enhances the osteogenic differentiation of both human and mouse mesenchymal stem cells induced by Cbfa-1 via reducing its ubiquitination-mediated degradation
    Chun Yang Lien
    Institute of Biopharmaceutical Science, National Yang Ming University, Shi Pai, Taipei 11221, Taiwan, Republic of corrected China
    Stem Cells 25:1462-8. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  36. pmc 17β-estradiol inhibits mesenchymal stem cells-induced human AGS gastric cancer cell mobility via suppression of CCL5- Src/Cas/Paxillin signaling pathway
    Chao Hung Kuo
    1 Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan 2 Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan 3 Department of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    Int J Med Sci 11:7-16. 2014
    ..Collectively, these results suggest that 17β-estradiol treatment significantly inhibits HBMMSCS-induced mobility in human AGS gastric cancer cells. ..
  37. pmc Evaluation of lentiviral-mediated expression of sodium iodide symporter in anaplastic thyroid cancer and the efficacy of in vivo imaging and therapy
    Chien Chih Ke
    Institute of Clinical Medicine, National Yang Ming University, Taipei 11221, Taiwan
    J Oncol 2011:178967. 2011
    ..Our results suggest that the lentiviral system efficiently transferred and expressed hNIS gene in ATC cells. The transduced cells showed a promising result of tumor imaging and therapy...
  38. doi request reprint A facile technique to make articulating spacers for infected total knee arthroplasty
    Yu Ping Su
    Department of Orthopedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China
    J Chin Med Assoc 72:138-45. 2009
    ..The current study was conducted to test their clinical efficacy...
  39. doi request reprint A tumorigenic homeobox (HOX) gene expressing human gastric cell line derived from putative gastric stem cell
    Yuan Chieh Yang
    Department of Laboratory Medicine, Graduate Institute of Medicine, I Shou University, Taipei, Taiwan
    Eur J Gastroenterol Hepatol 21:1016-23. 2009
    ..Study the mechanism of gastric tumor development...
  40. doi request reprint Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering
    Wei Cheng Jiang
    Institute of Biomedical Engineering, National Yang Ming University, Taiwan Stem Cell Research Center, National Yang Ming University, Taiwan Electronic address
    Biomaterials 35:3607-17. 2014
    ....
  41. doi request reprint Cannabinoid receptors as therapeutic targets for dialysis-induced peritoneal fibrosis
    Chih Yu Yang
    Division of Nephrology, Taipei Veterans General Hospital, Taipei, Taiwan, ROC
    Am J Nephrol 37:50-8. 2013
    ..As cannabinoid receptor (CBR) ligands have been reported to be beneficial to ameliorate the process of liver fibrosis, we strove to investigate their therapeutic potential to prevent peritoneal fibrosis...