Tung Kung Wu

Summary

Affiliation: National Chiao Tung University
Country: Taiwan

Publications

  1. doi request reprint Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterol's C/D-ring cyclization and 17α/β-exocyclic side chain stereochemistry
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Biomol Chem 9:1092-7. 2011
  2. doi request reprint Alteration of the substrate's prefolded conformation and cyclization stereochemistry of oxidosqualene-lanosterol cyclase of Saccharomyces cerevisiae by substitution at phenylalanine 699
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Lett 12:500-3. 2010
  3. doi request reprint Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: a chemistry-biology interdisciplinary study of the protein's structure-function-reaction mechanism relationships
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 75, Po Ai Street, Hsin Chu 300, Taiwan, ROC
    Chem Rec 8:302-25. 2008
  4. doi request reprint Importance of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase tyrosine 707 residue for chair-boat bicyclic ring formation and deprotonation reactions
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Lett 10:4959-62. 2008
  5. doi request reprint Protein plasticity: a single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien-3beta-ol, a rearrangement product
    Tung Kung Wu
    Department of Biological Science and Technology, Institute of Molecular Science, National Chiao Tung University, Hsin Chu, Taiwan, ROC
    Org Lett 10:2529-32. 2008
  6. pmc Identification of a Vibrio furnissii oligopeptide permease and characterization of its in vitro hemolytic activity
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, 300, Republic of China
    J Bacteriol 189:8215-23. 2007
  7. ncbi request reprint Phenylalanine 445 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences C-Ring cyclization and deprotonation reactions
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Lett 8:4691-4. 2006
  8. ncbi request reprint Site-saturated mutagenesis of histidine 234 of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase demonstrates dual functions in cyclization and rearrangement reactions
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan Republic of China
    J Am Chem Soc 128:6414-9. 2006
  9. ncbi request reprint Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan, China
    Arch Biochem Biophys 421:42-53. 2004
  10. ncbi request reprint Histidine residue at position 234 of oxidosqualene-lanosterol cyclase from saccharomyces cerevisiae simultaneously influences cyclization, rearrangement, and deprotonation reactions
    Tung Kung Wu
    Department of Biological Science and Technology, Center for Interdisciplinary Molecular Science, National Chiao Tung University, 300 Hsin Chu, Taiwan, Republic of China
    Chembiochem 6:1177-81. 2005

Collaborators

  • Chun Jung Chen
  • Yu Ju Chen
  • John H Griffin
  • Tony J Fang
  • Cheng Hsiang Chang
  • Yu Kuo Wang
  • Yan Ren Lin
  • Chin Yuan Chang
  • Yuan Ting Liu
  • Wen Hung Chen
  • I Chuan Sheih
  • Ting Yi Wang
  • Yi Fang Wu
  • Manoswini Nayak
  • Sheng Cih Huang
  • Chin Hao Chang
  • Liyang Luo
  • Hsu Hua Yeh
  • Wen Hsuan Li
  • Wen Shiang Shie
  • Hao Yu Wen
  • Tzu An Liu
  • Thomas Tien Hsiung Li
  • Yin Cheng Hsieh
  • Eric Wei Guang Diau
  • Su Hua Huang
  • I hsiang Hsu
  • Bak Sau Yip
  • Kenneth S Bruno
  • Berl R Oakley
  • Clay C C Wang
  • Keh Bin Wang
  • Ching Ting Lu
  • Shu Lin Chang
  • Meng Erh Li
  • Yi Ming Chiang
  • Yuh Chang Sun
  • Sheng Wei Tseng
  • Cheng Yu Ko
  • Chiao Ying Huang
  • Tsai Ting Wang
  • Tain Chang Hu
  • Chen Fu Lo
  • Hung Ming Lin
  • Han Ping Wu
  • Yi Rong Chiu
  • Yi Ting Hu
  • Ching Yao Lin
  • Chu Chung Chou
  • Yen Ling Lin
  • Yan Ling Lin
  • Bo Sou Yeh
  • Ru Yin Chen
  • Ting Wei Chiang
  • Liang Wei Kao
  • Jen Min Feng
  • Yen Hsi Liu

Detail Information

Publications30

  1. doi request reprint Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterol's C/D-ring cyclization and 17α/β-exocyclic side chain stereochemistry
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Biomol Chem 9:1092-7. 2011
    ..These results indicated the importance of the Ile705 residue in affecting lanosterol's C/D ring stabilization including 6-6-5 tricyclic and protosteryl C-17 cations and 17α/β-exocyclic side chain stereochemistry...
  2. doi request reprint Alteration of the substrate's prefolded conformation and cyclization stereochemistry of oxidosqualene-lanosterol cyclase of Saccharomyces cerevisiae by substitution at phenylalanine 699
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Lett 12:500-3. 2010
    ....
  3. doi request reprint Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: a chemistry-biology interdisciplinary study of the protein's structure-function-reaction mechanism relationships
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 75, Po Ai Street, Hsin Chu 300, Taiwan, ROC
    Chem Rec 8:302-25. 2008
    ....
  4. doi request reprint Importance of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase tyrosine 707 residue for chair-boat bicyclic ring formation and deprotonation reactions
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Lett 10:4959-62. 2008
    ....
  5. doi request reprint Protein plasticity: a single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien-3beta-ol, a rearrangement product
    Tung Kung Wu
    Department of Biological Science and Technology, Institute of Molecular Science, National Chiao Tung University, Hsin Chu, Taiwan, ROC
    Org Lett 10:2529-32. 2008
    ..The results suggest that Phe699Thr mutation is likely to affect the C-17 cation stabilization during the rearrangement process...
  6. pmc Identification of a Vibrio furnissii oligopeptide permease and characterization of its in vitro hemolytic activity
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, 300, Republic of China
    J Bacteriol 189:8215-23. 2007
    ..Finally, the in vitro hemolytic activity was also confirmed with homologous ATP-binding cassette-type transporter proteins from other Vibrio species...
  7. ncbi request reprint Phenylalanine 445 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences C-Ring cyclization and deprotonation reactions
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Lett 8:4691-4. 2006
    ....
  8. ncbi request reprint Site-saturated mutagenesis of histidine 234 of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase demonstrates dual functions in cyclization and rearrangement reactions
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan Republic of China
    J Am Chem Soc 128:6414-9. 2006
    ..Specifically, subtle changes in the active site affect both the stability of the cation-pi interaction and generate product diversity...
  9. ncbi request reprint Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan, China
    Arch Biochem Biophys 421:42-53. 2004
    ..The stoichiometry of histidine modification and the extent of inactivation showed that two essential histidine residues per active site are necessary for complete bovine liver OSC activity...
  10. ncbi request reprint Histidine residue at position 234 of oxidosqualene-lanosterol cyclase from saccharomyces cerevisiae simultaneously influences cyclization, rearrangement, and deprotonation reactions
    Tung Kung Wu
    Department of Biological Science and Technology, Center for Interdisciplinary Molecular Science, National Chiao Tung University, 300 Hsin Chu, Taiwan, Republic of China
    Chembiochem 6:1177-81. 2005
  11. ncbi request reprint Enzymatic formation of multiple triterpenes by mutation of tyrosine 510 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae
    Tung Kung Wu
    Department of Biological Science and Technology, Center for Interdisciplinary Molecular Science, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Chembiochem 5:1712-5. 2004
  12. pmc Crystal structure and mutational analysis of aminoacylhistidine dipeptidase from Vibrio alginolyticus reveal a new architecture of M20 metallopeptidases
    Chin Yuan Chang
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30010, Taiwan
    J Biol Chem 285:39500-10. 2010
    ....
  13. doi request reprint The cysteine 703 to isoleucine or histidine mutation of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae generates an iridal-type triterpenoid
    Cheng Hsiang Chang
    Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan
    Biochimie 94:2376-81. 2012
    ....
  14. doi request reprint Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus
    Ting Yi Wang
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, China
    FEBS J 275:5007-20. 2008
    ..Taken together, these results may facilitate the design of PepD inhibitors for application in antimicrobial treatment and antibody-directed enzyme prodrug therapy...
  15. doi request reprint Protein engineering of oxidosqualene-lanosterol cyclase into triterpene monocyclase
    Cheng Hsiang Chang
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Biomol Chem 11:4214-9. 2013
    ....
  16. pmc The thermostable direct hemolysin from Grimontia hollisae causes acute hepatotoxicity in vitro and in vivo
    Yan Ren Lin
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China
    PLoS ONE 8:e56226. 2013
    ..Secondary liver injury might be caused by venous return of the toxin through the portal system. We aimed to firstly analyze the in vitro and in vivo hepatotoxicity of Gh-TDH...
  17. pmc Site-directed mutations of thermostable direct hemolysin from Grimontia hollisae alter its arrhenius effect and biophysical properties
    Yu Kuo Wang
    Department of Biological Science and Technology, National Chiao Tung University, 30068, Hsin Chu, Taiwan, Republic of China
    Int J Biol Sci 7:333-46. 2011
    ..Consistent with the observation is the conformational change of the proteins from native globular form into fibrillar form, as determined by Congo red experiments and transmission electron microscopy...
  18. ncbi request reprint Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions
    Tung Kung Wu
    Department of Biological Science and Technology and Department of Applied Chemistry, National Chiao Tung University, 300 Hsin Chu, Taiwan, Republic of China
    Org Lett 8:1319-22. 2006
    ..Overall, Trp232 plays a catalytic role in the influence of rearrangement process and determination of deprotonation position but does not involve intervention in the cyclization steps...
  19. doi request reprint Protein engineering of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase
    Yuan Ting Liu
    Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin Chu, Taiwan, Republic of China
    Org Lett 14:5222-5. 2012
    ..cerevisiae...
  20. ncbi request reprint Conversion of a plant oxidosqualene-cycloartenol synthase to an oxidosqualene-lanosterol cyclase by random mutagenesis
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, 300, Taiwan, Republic of China
    Biochemistry 41:8238-44. 2002
    ..Sequence and structural homology modeling of CAS indicate that the observed product specificity-altering mutations occur both within (Tyr410Cys, Ile481Thr, and Tyr532His) and outside of (Ala469Val and His477Tyr) the cyclase active site...
  21. pmc Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae
    Yu Kuo Wang
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:224-7. 2011
    ..4 and 2.3 Å(3) Da(-1), respectively. These crystals were suitable for structure determination, which would yield structural details related to the cytotoxicity and oligomeric structure of this pore-forming toxin...
  22. ncbi request reprint Modified colorimetric assay for uricase activity and a screen for mutant Bacillus subtilis uricase genes following StEP mutagenesis
    Su Hua Huang
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China
    Eur J Biochem 271:517-23. 2004
    ..1 U.mg(-1)). Finally, the modified colorimetric method is much more efficient than the conventional ones and greatly reduces assay time from 4 days to less than 20 h...
  23. pmc Photoactivation studies of zinc porphyrin-myoglobin system and its application for light-chemical energy conversion
    Chin Hao Chang
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China
    Int J Biol Sci 7:1203-13. 2011
    ....
  24. pmc Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping
    Hsu Hua Yeh
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu City 30010, Taiwan
    Org Lett 15:756-9. 2013
    ..Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes...
  25. doi request reprint Immunocapture couples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid detection of type 1 dengue virus
    Wen Hung Chen
    Department of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan
    J Chromatogr A 1288:21-7. 2013
    ..The immunocapture could unambiguously differentiate the DV1 from other serotypes of the dengue viruses and Japanese encephalitis virus, and could be used as a specific probe to detect DV1 from complex biological mixtures...
  26. pmc Purification, crystallization and preliminary X-ray analysis of an aminoacylhistidine dipeptidase (PepD) from Vibrio alginolyticus
    Chin Yuan Chang
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:216-8. 2009
    ..42, c = 303.11 A. The crystal contained two molecules per asymmetric unit and the predicted solvent content was 53.4%...
  27. ncbi request reprint Ultrafast relaxation of zinc protoporphyrin encapsulated within apomyoglobin in buffer solutions
    Liyang Luo
    Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, Hsinchu 30010, Taiwan
    J Phys Chem B 111:7656-64. 2007
    ..The transfer of energy through bonds might lead the dative bond to break, which explains our observation of the degradation of ZnPP-Mb samples in UV-vis and CD spectra upon protracted excitation...
  28. doi request reprint Poison exposure and outcome of children admitted to a pediatric emergency department
    Yan Ren Lin
    Department of Emergency Medicine, Changhua Christian Hospital, Changhua, Taiwan, China
    World J Pediatr 7:143-9. 2011
    ..This paper reports the characteristics, outcomes and clinical features of children with poisoning treated at an emergency department (ED)...
  29. doi request reprint Purification and properties of a novel phenolic antioxidant from Radix astragali fermented by Aspergillus oryzae M29
    I Chuan Sheih
    Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan, Republic of China
    J Agric Food Chem 59:6520-5. 2011
    ..These data demonstrated that the solid-state bioprocessing strategy could be an innovative approach to enhance the antioxidant activity of RA...
  30. doi request reprint Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems
    I Chuan Sheih
    Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, ROC
    Bioresour Technol 100:3419-25. 2009
    ..These results demonstrate that inexpensive algae protein waste could be a new alternative to produce antioxidative peptides...