Tzong Yuan Wu

Summary

Affiliation: Chung Yuan Christian University
Country: Taiwan

Publications

  1. pmc A bi-cistronic baculovirus expression vector for improved recombinant protein production
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chungli, Taiwan
    Bioeng Bugs 3:129-32. 2012
  2. pmc Cell-based analysis of Chikungunya virus E1 protein in membrane fusion
    Szu Cheng Kuo
    Division of Microbiology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan
    J Biomed Sci 19:44. 2012
  3. doi request reprint Dual action of memantine in Alzheimer disease: a hypothesis
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chungli, Taiwan
    Taiwan J Obstet Gynecol 48:273-7. 2009
  4. pmc IRSS: a web-based tool for automatic layout and analysis of IRES secondary structure prediction and searching system in silico
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taiwan
    BMC Bioinformatics 10:160. 2009
  5. doi request reprint Alzheimer's disease: aging, insomnia and epigenetics
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taipei, Taichung, Taiwan
    Taiwan J Obstet Gynecol 49:468-72. 2010
  6. doi request reprint Cell-based analysis of Chikungunya virus membrane fusion using baculovirus-expression vectors
    Szu Cheng Kuo
    Division of Microbiology, Graduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan 333, Taiwan
    J Virol Methods 175:206-15. 2011
  7. doi request reprint Effects of curcumin and demethoxycurcumin on amyloid-β precursor and tau proteins through the internal ribosome entry sites: a potential therapeutic for Alzheimer's disease
    Oliver B Villaflores
    Department of Chemistry, Chung Yuan Christian University, Chung Li, Taiwan
    Taiwan J Obstet Gynecol 51:554-64. 2012
  8. ncbi request reprint Development of a bi-cistronic baculovirus expression vector by the Rhopalosiphum padi virus 5' internal ribosome entry site
    Ying Ju Chen
    Department of Bioscience Technology, Chung Yuan Christian University, Chungli 320, Taiwan, ROC
    Biochem Biophys Res Commun 335:616-23. 2005
  9. ncbi request reprint Coral red fluorescence protein as genetic modified baculovirus tracer
    Tzyy Rong Jinn
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
    J Biotechnol 119:255-9. 2005
  10. ncbi request reprint The 5' untranslated region of Perina nuda virus (PnV) possesses a strong internal translation activity in baculovirus-infected insect cells
    Tzong Yuan Wu
    Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, Chung Li, Taiwan
    FEBS Lett 581:3120-6. 2007

Collaborators

  • Yu Chen Hu
  • Shin Ru Shih
  • Suey Sheng Kao
  • Chi Ming Wu
  • Jin Ching Lee
  • Monique M van Oers
  • Chung Hsiung Wang
  • Chih Ping Chen
  • Ying Ju Chen
  • Oliver B Villaflores
  • Tzyy Rong Jinn
  • Chao Yi Teng
  • Szu Cheng Kuo
  • Jui Ming Yeh
  • Jun Jie Hong
  • Pei Yi Tsui
  • Yung Hsin Hsu
  • Kuo Lun Tung
  • Ming Der Kuo
  • Yu Ming Wang
  • Szecheng J Lo
  • Yen Chung Chang
  • Sheng Kuo Hsieh
  • Evangelyn Dominguez
  • Yen Kuang Li
  • Yi jane Wu
  • Jason T C Tzen
  • Jiunn Jye Wey
  • Der Jiang Chiao
  • Chein Ming Hsei
  • Tsair Yuan Chang
  • Rong Hwa Shyu
  • Wen Shuo Cheng
  • Hui Wen Chung
  • Hui Ing Wu
  • Chun Fa Huang
  • Wei Hung Yang
  • Ting Yu Chin
  • Feng Chia Hsieh
  • Wen Shuo Chen
  • Yin Chin Tseng
  • Yui Whei Chen-Yang
  • Yi Ting Lin
  • Shih jhan Zeng
  • Jim Tong Horng
  • Seng chi Chen
  • Yu ting Chu
  • Yu Jie Chen
  • Mei Ju Chou
  • Hong ming Yang
  • John Ta Hsu
  • Wein Shue Chen

Detail Information

Publications25

  1. pmc A bi-cistronic baculovirus expression vector for improved recombinant protein production
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chungli, Taiwan
    Bioeng Bugs 3:129-32. 2012
    ....
  2. pmc Cell-based analysis of Chikungunya virus E1 protein in membrane fusion
    Szu Cheng Kuo
    Division of Microbiology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan
    J Biomed Sci 19:44. 2012
    ..How the E1 structure determines membrane fusion and whether other CHIKV structural proteins participate in E1 fusion activity remain largely unexplored...
  3. doi request reprint Dual action of memantine in Alzheimer disease: a hypothesis
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chungli, Taiwan
    Taiwan J Obstet Gynecol 48:273-7. 2009
    ..Memantine may reduce the expression of amyloid precursor protein and tau protein, as well as acting as an antagonist of N-methyl-D-aspartate receptors in the brain...
  4. pmc IRSS: a web-based tool for automatic layout and analysis of IRES secondary structure prediction and searching system in silico
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taiwan
    BMC Bioinformatics 10:160. 2009
    ..IRES elements are important factors in viral genomes and are also useful tools for bi-cistronic expression vectors. Most existing RNA structure prediction programs are unable to deal with IRES elements...
  5. doi request reprint Alzheimer's disease: aging, insomnia and epigenetics
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taipei, Taichung, Taiwan
    Taiwan J Obstet Gynecol 49:468-72. 2010
    ..This brief review focuses on new studies that demonstrate the link between epigenetics and AD, and explores the possibility that insomnia may be one factor that effects AD...
  6. doi request reprint Cell-based analysis of Chikungunya virus membrane fusion using baculovirus-expression vectors
    Szu Cheng Kuo
    Division of Microbiology, Graduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan 333, Taiwan
    J Virol Methods 175:206-15. 2011
    ..It was also demonstrated that the heterologous expression of alphavirus monomeric E1 can induce insect cell fusions. Furthermore, this cell-based system provides a model for studying class II viral membrane fusion...
  7. doi request reprint Effects of curcumin and demethoxycurcumin on amyloid-β precursor and tau proteins through the internal ribosome entry sites: a potential therapeutic for Alzheimer's disease
    Oliver B Villaflores
    Department of Chemistry, Chung Yuan Christian University, Chung Li, Taiwan
    Taiwan J Obstet Gynecol 51:554-64. 2012
    ....
  8. ncbi request reprint Development of a bi-cistronic baculovirus expression vector by the Rhopalosiphum padi virus 5' internal ribosome entry site
    Ying Ju Chen
    Department of Bioscience Technology, Chung Yuan Christian University, Chungli 320, Taiwan, ROC
    Biochem Biophys Res Commun 335:616-23. 2005
    ....
  9. ncbi request reprint Coral red fluorescence protein as genetic modified baculovirus tracer
    Tzyy Rong Jinn
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
    J Biotechnol 119:255-9. 2005
    ..Insect larvae, including Trichoplusia ni, Spodoptera exigua, and Spodoptera litura, infected the GMBV containing the DsRed gene can emit red fluorescence under sun light without any prosthetic apparatus...
  10. ncbi request reprint The 5' untranslated region of Perina nuda virus (PnV) possesses a strong internal translation activity in baculovirus-infected insect cells
    Tzong Yuan Wu
    Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, Chung Li, Taiwan
    FEBS Lett 581:3120-6. 2007
    ....
  11. doi request reprint Easy expression of the C-terminal heavy chain domain of botulinum neurotoxin serotype A as a vaccine candidate using a bi-cistronic baculovirus system
    Oliver B Villaflores
    Department of Chemistry, Chung Yuan Christian University, Chungli 32023, Taiwan
    J Virol Methods 189:58-64. 2013
    ..2μg. This study provided supportive evidence for the use of a bi-cistronic baculovirus-Sf21 insect cell expression system in the facile expression of an immunogenically active rBoNT/A-HC...
  12. ncbi request reprint High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site
    Jin Ching Lee
    Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, 128 Yen Chiu Yuan Road, Sec 2, Taipei, Taiwan, Republic of China
    Biotechnol Bioeng 90:656-62. 2005
    ..Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector...
  13. doi request reprint Additive effect of calreticulin and translation initiation factor eIF4E on secreted protein production in the baculovirus expression system
    Chao Yi Teng
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, 320, Taiwan, Republic of China
    Appl Microbiol Biotechnol 97:8505-16. 2013
    ..Utilization of this "triple-supporters" containing vector offers significant advantages when producing secreted proteins and is likely to have benefits for the production of viral vaccines and other pharmaceutical products. ..
  14. doi request reprint High-level expression, purification and production of the fungal immunomodulatory protein-gts in baculovirus-infected insect larva
    Tzong Yuan Wu
    Department of Science Technology, Chung Yuan Christian University, Chung Li, Taiwan 32023, Republic of China
    Appl Biochem Biotechnol 169:976-89. 2013
    ..Our approach will open a new avenue for the production of rFip-gts and facilitate the immunoregulatory activity of rFip-gts available in the future...
  15. doi request reprint Internal ribosome entry site of Rhopalosiphum padi virus is functional in mammalian cells and has cryptic promoter activity in baculovirus-infected Sf21 cells
    Yi jane Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chungli 320, Taiwan, China
    Acta Pharmacol Sin 29:965-74. 2008
    ....
  16. ncbi request reprint Secretory fluorescent protein, a secretion green fluorescent fusion protein with alkaline phosphatase activity as a sensitive and traceable reporter in baculovirus expression system
    Chao Yi Teng
    Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, Chung Li, Taiwan
    Biotechnol Lett 29:1019-24. 2007
    ..This SEFP reporter gene with traceable fluorescence and alkaline phosphatase activity may become a useful tool for studies on secretory protein production...
  17. doi request reprint Curcuminoids and resveratrol as anti-Alzheimer agents
    Oliver B Villaflores
    Department of Chemistry, Chung Yuan Christian University, Chung Li, Taiwan
    Taiwan J Obstet Gynecol 51:515-25. 2012
    ..This review article revisits and presents the current research done on the potential of the curcuminoids curcumin and demethoxycurcumin and the polyphenolic compound resveratrol as anti-AD compounds...
  18. doi request reprint Management of moderate to severe Alzheimer's disease: focus on memantine
    Evangelyn Dominguez
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taiwan
    Taiwan J Obstet Gynecol 50:415-23. 2011
    ....
  19. doi request reprint Amantadine as a regulator of internal ribosome entry site
    Ying Ju Chen
    Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, Chung Li, Taiwan, China
    Acta Pharmacol Sin 29:1327-33. 2008
    ..We hypothesized that it might be possible to identify compounds that may distinguish between cap-dependent translation and cap-independent IRES-mediated translation...
  20. ncbi request reprint Functional expression and characterization of dipeptidyl peptidase IV from the black-bellied hornet Vespa basalis in Sf21 insect cells
    Sheng Kuo Hsieh
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
    Biosci Biotechnol Biochem 75:2371-5. 2011
    ..The optimal temperature and pH for rDPP-IV were determined to be 50 °C and pH 9. Enzymatic activity of rDPP-IV was significantly reduced by 80 and 60% in the presence of sitagliptin and phenylmethylsulfonyl fluoride respectively...
  21. doi request reprint Traditional Chinese medicines and Alzheimer's disease
    Tzong Yuan Wu
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taiwan
    Taiwan J Obstet Gynecol 50:131-5. 2011
    ....
  22. doi request reprint Aerosol infectivity of a Baculovirus to Trichoplusia ni larvae: An alternative larval inoculation strategy for recombinant protein production
    Tzyy Rong Jinn
    Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan
    Biotechnol Prog 25:384-9. 2009
    ..ni larvae following aerosol infection were nearly identical to those following oral feeding or injection. This alternative baculovirus infection strategy facilitates recombinant protein and virus production by insect larvae...
  23. pmc Viral IRES prediction system - a web server for prediction of the IRES secondary structure in silico
    Jun Jie Hong
    Department of Bioscience Technology, Chung Yuan Christian University, Chung Li, Taiwan
    PLoS ONE 8:e79288. 2013
    ..36% and 80.41% for IRES groups 1, 2, 3, and 4, respectively. This advance useful search approach for IRES structures will facilitate IRES related studies. The VIPS on-line website service is available at http://140.135.61.250/vips/. ..
  24. ncbi request reprint A novel method for preparing a protein-encapsulated bioaerogel: using a red fluorescent protein as a model
    Yen Kuang Li
    Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung Li, Taoyuan County 32023, Taiwan, ROC
    Acta Biomater 4:725-32. 2008
    ....
  25. doi request reprint A chip-based method for studying the effects of exogenous proteins on neuronal axons
    Yung Hsin Hsu
    Institute of Molecular Medicine, National Tsing Hua University, Hsinchu 300, Taiwan, ROC
    Anal Biochem 427:1-9. 2012
    ..These results indicate the diverse applications of the chip device in exploring various issues pertaining to axonal functions...