Research Topics
Genomes and Genes | Albert M WuSummaryAffiliation: Chang Gung University Country: Taiwan Publications
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Publications
Polyvalent GalNAcalpha1-->Ser/Thr (Tn) and Galbeta1-->3GalNAcalpha1-->Ser/Thr (T alpha) as the most potent recognition factors involved in Maclura pomifera agglutinin-glycan interactionsAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
J Biomed Sci 12:135-52. 2005..Our findings should aid in the selection of this lectin for elucidating functions of carbohydrate chains in life processes and for applications in the biomedical sciences...
Recognition factors of Ricinus communis agglutinin 1 (RCA(1))Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
Mol Immunol 43:1700-15. 2006..These results should be helpful for understanding the functional role of RCA1 and for characterizing glycotopes of mammalian complex carbohydrates...
Recognition intensities of submolecular structures, mammalian glyco-structural units, ligand cluster and polyvalency in abrin-a-carbohydrate interactionsJune H Wu
Department of Microbiology and Immunology, Chang Gung University, Tao Yuan, Taiwan
Biochimie 92:147-56. 2010..A comparison of the recognition factors and combining sites of abrin-a with ricin, another highly toxic lectin, was also performed to further understand the differences in recognition factors between these two type 2 RIPs...
Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating LFucα1-->, DManα1--> and Galβ1-->3/4GlcNAc glycotopesAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
FEBS Lett 586:1294-9. 2012..The peculiar recognition systems of RSL are very different from other well known microbial lectins...
Lectins as tools in glycoconjugate researchAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Taoyuan 333, Taiwan
Glycoconj J 26:899-913. 2009..Some chemical modifications of lectin ligands on the microtiter plates and blots (desialylation, Smith degradation, beta-elimination), which extend the applicability of these methods, are also described...
Further characterization of the binding properties of two monoclonal antibodies recognizing human Tn red blood cellsAlbert M Wua
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Taiwan
J Biomed Sci 12:153-66. 2005..Identification of these binding properties should aid in the selection of these MoAbs and the conditions required for biological studies and clinical applications...
Lectinochemical studies on the glyco-recognition factors of a Tn (GalNAcalpha1-->Ser/Thr) specific lectin isolated from the seeds of Salvia sclareaAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
J Biomed Sci 12:167-84. 2005..The effect of the polyvalent F alpha, F beta and GalNAc beta1-->3Gal alpha1--> (P alpha) glycotopes on binding should be examined. However, this is hampered by the lack of availability of suitable reagents...
Lectinochemical studies on the binding properties of a toxic lectin (ricin) isolated from the seeds of Ricinus communisAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Taoyuan, Taiwan, ROC
Chang Gung Med J 28:530-42. 2005..Ricin (RCA2 or RCA60) is a highly toxic heterodimeric protein found in the seeds of the castor plant Ricinus communis. It is a potential biohazard. In the present study, the fine specificity of ricin was defined...
Interaction profile of galectin-5 with free saccharides and mammalian glycoproteins: probing its fine specificity and the effect of naturally clustered ligand presentationAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan, 333, Taiwan
Glycobiology 16:524-37. 2006..These results are important for the systematic comparison of ligand selection in this family of adhesion/growth-regulatory effectors with potential for medical applications...
Interactions of the fucose-specific Pseudomonas aeruginosa lectin, PA-IIL, with mammalian glycoconjugates bearing polyvalent Lewis(a) and ABH blood group glycotopesA M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
Biochimie 88:1479-92. 2006..Such information might be useful for the antiadhesive therapy of P. aeruginosa infections...
Activity-structure correlations in divergent lectin evolution: fine specificity of chicken galectin CG-14 and computational analysis of flexible ligand docking for CG-14 and the closely related CG-16Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Glycobiology 17:165-84. 2007..In summary, this study identifies activity differences and provides information on their relation to structural divergence, epitomizing the value of this combined approach beyond galectins...
Differential affinities of Erythrina cristagalli lectin (ECL) toward monosaccharides and polyvalent mammalian structural unitsAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
Glycoconj J 24:591-604. 2007..These analyses should facilitate the understanding of the binding function of ECL...
Differential contributions of recognition factors of two plant lectins -Amaranthus caudatus lectin and Arachis hypogea agglutinin, reacting with Thomsen-Friedenreich disaccharide (Galbeta1-3GalNAcalpha1-Ser/Thr)Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University Kwei san, Tao Yuan, Taiwan
Biochimie 90:1769-80. 2008....
Multivalent human blood group ABH and Lewis glycotopes are key recognition factors for a LFuc>Man binding lectin from phytopathogenic Ralstonia solanacearumAlbert M Wu
Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Biochim Biophys Acta 1790:249-59. 2009..The outstandingly broad RSL saccharide-binding profile might be related to the unusually wide spectrum of plants that suffer from R. solanacearum pathogenicity and provide ideas for protective antiadhesion strategies...
Roles of mammalian structural units, ligand cluster and polyvalency in the Abrus precatorius agglutinin and glycoprotein recognition processAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Mol Immunol 46:3427-37. 2009..A comparison of the differential recognition factors and combining sites of APA with those of other lectins (Ricinus communis agglutinin, RCA(1) and ricin) is also illustrated...
Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)Albert M Wu
Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
FEBS Lett 584:2371-5. 2010..This finding provides a peculiar concept of the duality of PA-IIL recognition system for LFucalpha1--> and related complexes and for high density Manalpha1--> complexes present in polymannosylated target macromolecules...
Multiple recognition systems adopting four different glycotopes at the same domain for the Agaricus bisporus agglutinin-glycan interactionsAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
FEBS Lett 584:3561-6. 2010..This peculiar concept provides the most comprehensive mechanism for the attachment of ABA to target glycans and malignant cells at the molecular level...
A novel lectin (Morniga M) from mulberry (Morus nigra) bark recognizes oligomannosyl residues in N-glycansAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kweishan, Taoyuan, Taiwan
J Biomed Sci 11:874-85. 2004....
Lectinochemical studies on the affinity of Anguilla anguilla agglutinin for mammalian glycotopesAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan, 333, Taiwan
Life Sci 75:1085-103. 2004..From the present observations, it can be concluded that the combining site of AAA should be a small cavity-type capable of recognizing mainly H/crypto H and of binding to specific polyvalent ABH and Leb glycotopes...
Effect of polyvalencies of glycotopes on the binding of a lectin from the edible mushroom, Agaricus bisporusAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
Biochem J 371:311-20. 2003....
Carbohydrate specificity of a galectin from chicken liver (CG-16)A M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, 259 Wen Hwa 1st Road, Kwei San, Tao Yuan 333, Taiwan
Biochem J 358:529-38. 2001..The binding site of CG-16 is as large as a tetrasaccharide of the beta-anomer of Gal, and is most complementary to lacto-N-tetraose and Gal(beta1-4)GlcNAc related sequences...
Carbohydrate specificity of a toxic lectin, abrin A, from the seeds of Abrus precatorius (jequirity bean)A M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Life Sci 69:2027-38. 2001..These active glycotopes can be used to explain the possible structural requirements for abrin A toxin attachment...
Defining the carbohydrate specificities of aplysia gonad lectin exhibiting a peculiar D-galacturonic acid affinityA M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, School of Medicine, Chang Gung University, Kwei san 33332, Taiwan
J Biol Chem 275:14017-24. 2000..From the data provided, the carbohydrate specificity of AGL can be defined as GalUAalpha1-->4 trisaccharides to mono GalUA > branched or cluster forms of E, I, and II monomeric E, I, and II, whereas GalNAc is inactive...
Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsiiA M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Life Sci 69:2039-50. 2001..The affinity of SRL for ligands can be ranked in decreasing order as follows: multivalent T(alpha) and Tn >> monomeric T(alpha) and Tn > GalNAc >>> II (Gal beta1-->4GlcNAc), L (Gal beta1-->4Glc), and Gal...
Fine specificity of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N)Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan, 333, Taiwan
Biochem J 367:653-64. 2002..The definition of this binding profile provides the basis to detect differential binding properties relative to the other galectins with ensuing implications for functional analysis...
Carbohydrate structural units in glycosphingolipids as receptors for Gal and GalNAc reactive lectinsAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Neurochem Res 27:593-600. 2002..Hence, the binding relation between GSLs and Gal/GalNAc specific lectins can be established...
Forssman pentasaccharide and polyvalent Galbeta1-->4GlcNAc as major ligands with affinity for Caragana arborescens agglutininA M Wu
Glyco Immunochemistry Research Lab, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
FEBS Lett 463:225-30. 1999..This property can be used to detect human abnormal glycotopes related to F(p) and unmasked mII/Tn clusters and to study cell growth and differentiation given the lack of toxicity of this lectin toward mouse fibroblast cells...
Binding profile of Artocarpus integrifolia agglutinin (Jacalin)Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan, 333, Taiwan
Life Sci 72:2285-302. 2003..Gt; Man and Glc (inactive). Our finding should aid in the selection of this lectin for biological applications...
Effects of polyvalency of glycotopes and natural modifications of human blood group ABH/Lewis sugars at the Galbeta1-terminated core saccharides on the binding of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N)Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Kwei San, Tao Yuan 333, Taiwan, ROC
Biochimie 86:317-26. 2004....
Polyvalency of Tn (GalNAcalpha1-->Ser/Thr) glycotope as a critical factor for Vicia villosa B4 and glycoprotein interactionsAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
FEBS Lett 562:51-8. 2004....
Recognition profile of Bauhinia purpurea agglutinin (BPA)Albert M Wu
Glyco Immunochemistry Research Laboratory, Chang Gung Medical College, Institute of Molecular and Cellular Biology, Kwei San, Tao Yuan 333, Taiwan
Life Sci 74:1763-79. 2004..These findings give evidence for the binding of this lectin to dense cell surface T, Tn and I/II glycoconjugates and should facilitate future usage of this lectin in biotechnological and medical applications...
Carbohydrate structural units in glycoproteins and polysaccharides as important ligands for Gal and GalNAc reactive lectinsAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, 333 Taiwan
J Biomed Sci 10:676-88. 2003..Hence, the binding relationship between glycoproteins and Gal/GalNAc specific lectins can be explored...
Carbohydrate specificity of an agglutinin isolated from the root of Trichosanthes kirilowiiA M Wu
Glyco Immunochemistry Research Lab, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Life Sci 66:2571-81. 2000..The unusual affinity of TKA for terminal and internal Galbeta1-->glycotopes may be used to explain the possible attachment roles of this agglutinin in this folk medicine to target cells...
Recognition roles of the carbohydrate glycotopes of human and bovine lactoferrins in lectin-N-glycan interactionsMeng Hsiu Yen
Glyco Immunochemistry Research Labortory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Tao Yuan, Taiwan
Biochim Biophys Acta 1810:139-49. 2011..Previous studies showed that all lactoferrins are glycosylated in the human body, but the recognition roles of their carbohydrate glycotopes have not been well addressed...
Defining the carbohydrate specificities of Erythrina corallodendron lectin (ECorL) as polyvalent Galbeta1-4GlcNAc (II) > monomeric II > monomeric Gal and GalNAcZhangung Yang
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Gueishan Township, Taoyuan County 333, Taiwan, ROC
Chang Gung Med J 31:26-43. 2008..In previous studies, the carbohydrate specificities of this lectin were limited to monosaccharides, simple oligosaccharides and several clusters. However, the polyvalent factor has not been investigated...
Expression of sialyl Lex, sialyl Lea, Lex and Ley glycotopes in secreted human ovarian cyst glycoproteinsZhangung Yang
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
Biochimie 91:423-33. 2009....
Carbohydrate specificity of an insecticidal lectin isolated from the leaves of Glechoma hederacea (ground ivy) towards mammalian glycoconjugatesTanuja Singh
Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang-Gung University, Kwei-San, Tao-Yuan, 333, Taiwan
Biochem J 393:331-41. 2006..However, the extraordinary binding feature of Gleheda for glycoproteins demonstrates the importance of affinity enhancement by high-density multivalent glycotopes in the ligand-lectin interactions in biological processes...
Recognition profile of Morus nigra agglutinin (Morniga G) expressed by monomeric ligands, simple clusters and mammalian polyvalent glycotopesTanuja Singh
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
Mol Immunol 44:451-62. 2007..These results provide evidence for the binding of this lectin to dense cell surface T/Tn glycoconjugates and facilitate future usage of this lectin in biotechnological and medical applications...
Carbohydrate recognition factors of a Talpha (Galbeta1-->3GalNAcalpha1-->Ser/Thr) and Tn (GalNAcalpha1-->Ser/Thr) specific lectin isolated from the seeds of Artocarpus lakoochaTanuja Singh
Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung University, Kwei-San, Tao-Yuan, 333, Taiwan
Glycobiology 15:67-78. 2005....
Glycomic mapping of pseudomucinous human ovarian cyst glycoproteins: identification of Lewis and sialyl Lewis glycotopesAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Tao Yuan, Taiwan
Proteomics 7:3699-717. 2007....
Expression of binding properties of Gal/GalNAc reactive lectins by mammalian glycotopes (an updated report)A M Wu
Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine Chang-Gung University, Kwei-San, Tao-Yuan 333, Taiwan
Adv Exp Med Biol 491:55-64. 2001..To establish the relationship among the amino acid sequences of the combining sites of plant lectins and mammalian glycotopes should be an important direction to be addressed in lectinology...
A sheep hydatid cyst glycoprotein as receptors for three toxic lectins, as well as Abrus precatorius and Ricinus communis agglutininsA M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Kwei San, Taiwan
Biochim Biophys Acta 1243:124-8. 1995....
Native and/or asialo-Tamm-Horsfall glycoproteins Sd(a+) are important receptors for Triticum vulgaris (wheat germ) agglutinin and for three toxic lectins (abrin-a, ricin and mistletoe toxic lectin-I)A M Wu
Glyco Immunochemistry Research Laboratory, Chang Gung Medical College, Kwei San, Tao Yuan, Taiwan
FEBS Lett 371:32-4. 1995..From the above results, it is concluded that native and/or Tamm-Horsfall glycoproteins serve as important receptors for these three toxic lectins and for WGA...
Contribution of fucose-containing capsules in Klebsiella pneumoniae to bacterial virulence in miceJune Hsieh Wu
Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Tao Yuan 333, Taiwan
Exp Biol Med (Maywood) 233:64-70. 2008..The possession of fucose might contribute to KpL1 virulence by avoiding phagocytosis since fucose on bacteria had been implicated in immune evasion...
