Research Topics

Genomes and Genes


Chi Huey Wong


Affiliation: Academia Sinica
Country: Taiwan


  1. Shie J, Liu Y, Hsiao J, Fang J, Wong C. A cell-permeable and triazole-forming fluorescent probe for glycoconjugate imaging in live cells. Chem Commun (Camb). 2017;53:1490-1493 pubmed publisher
    ..This probe is cell-permeable and generates fluorescence after triazole formation, thus minimizing the background signal and enabling the real-time intracellular imaging of glycoconjugate trafficking. ..
  2. Tseng Y, Wu C, Liu M, Chen T, Chiang W, Yu Y, et al. Egg-based influenza split virus vaccine with monoglycosylation induces cross-strain protection against influenza virus infections. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
    ..This study provides a simple and practical procedure to enhance the cross-strain protection of influenza vaccine by removing the outer part of glycans from the virus surface through modifications of the current egg-based process. ..
  3. Lin C, Tsai M, Li S, Tsai T, Chu K, Liu Y, et al. A common glycan structure on immunoglobulin G for enhancement of effector functions. Proc Natl Acad Sci U S A. 2015;112:10611-6 pubmed publisher
  4. Chen J, Chen C, Ku K, Hsiao M, Chiang C, Hsu T, et al. Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response. Proc Natl Acad Sci U S A. 2015;112:13057-62 pubmed publisher
  5. Chuang P, Hsiao M, Hsu T, Chang C, Wu C, Chen B, et al. Signaling pathway of globo-series glycosphingolipids and β1,3-galactosyltransferase V (β3GalT5) in breast cancer. Proc Natl Acad Sci U S A. 2019;116:3518-3523 pubmed publisher
  6. Cheng C, Zhou Y, Pan W, Dey S, Wu C, Hsu W, et al. Hierarchical and programmable one-pot synthesis of oligosaccharides. Nat Commun. 2018;9:5202 pubmed publisher
    ..This advanced programmable one-pot method provides potential synthetic solutions for complex glycans with four successful examples demonstrated in this work. ..
  7. Wang S, Liao H, Zhang J, Cheng T, Wong C. Development of a universal influenza vaccine using hemagglutinin stem protein produced from Pichia pastoris. Virology. 2019;526:125-137 pubmed publisher
  8. Wang X, Krasnova L, Wu K, Wu W, Cheng T, Wong C. Towards new antibiotics targeting bacterial transglycosylase: Synthesis of a Lipid II analog as stable transition-state mimic inhibitor. Bioorg Med Chem Lett. 2018;28:2708-2712 pubmed publisher
    ..Compound 2b showed 70% inhibition of transglycosylase from Acinetobacter baumannii, providing a basis for further improvement. ..
  9. Lo P, Shie J, Chen C, Wu C, Hsu T, Wong C. O-GlcNAcylation regulates the stability and enzymatic activity of the histone methyltransferase EZH2. Proc Natl Acad Sci U S A. 2018;115:7302-7307 pubmed publisher
    ..Our results show the effect of individual O-GlcNAcylation sites on the function of EZH2 and suggest an alternative approach to tumor suppression through selective inhibition of EZH2 O-GlcNAcylation. ..

More Information


  1. Cheung S, Chuang P, Huang H, Hwang Verslues W, Cho C, Yang W, et al. Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells. Proc Natl Acad Sci U S A. 2016;113:960-5 pubmed publisher
  2. Liu C, Tsai T, Cheng T, Shivatare V, Wu C, Wong C. Glycoengineering of antibody (Herceptin) through yeast expression and in vitro enzymatic glycosylation. Proc Natl Acad Sci U S A. 2018;115:720-725 pubmed publisher
    ..The 10 newly identified recombinant bacterial endoglycosidases are complementary to existing endoglycosidases (EndoA, EndoH, EndoS), two of which can even accept sialylated tri- and tetraantennary glycans as substrates. ..
  3. Shivatare S, Shivatare V, Wu C, Wong C. Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling. J Vis Exp. 2018;: pubmed publisher
    ..In addition, our ACG array embodies a powerful tool to study other HIV antibodies for hetero-ligand binding behavior. ..
  4. Yeh S, Wang P, Lou Y, Khoo K, Hsiao M, Hsu T, et al. Glycolipid GD3 and GD3 synthase are key drivers for glioblastoma stem cells and tumorigenicity. Proc Natl Acad Sci U S A. 2016;113:5592-7 pubmed publisher
    ..Our results demonstrate that GD3 and GD3S are highly expressed in GSCs, play a key role in glioblastoma tumorigenicity, and are potential therapeutic targets against GBM. ..
  5. Wu C, Lin C, Tsai T, Lee C, Chuang H, Chen J, et al. Influenza A surface glycosylation and vaccine design. Proc Natl Acad Sci U S A. 2017;114:280-285 pubmed publisher