Michael M C Lai

Summary

Affiliation: Academia Sinica
Country: Taiwan

Publications

  1. pmc Hepatitis C virus translation preferentially depends on active RNA replication
    Helene Minyi Liu
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e43600. 2012
  2. pmc Transcription of subgenomic mRNA of hepatitis delta virus requires a modified hepatitis delta antigen that is distinct from antigenomic RNA synthesis
    Chung Hsin Tseng
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    J Virol 82:9409-16. 2008
  3. ncbi request reprint Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation
    Chao Kuen Lai
    Institute of Molecular Biology, Academia Sinica, Taipei, 115, Taiwan
    Virology 370:295-309. 2008
  4. pmc RNA-templated replication of hepatitis delta virus: genomic and antigenomic RNAs associate with different nuclear bodies
    YI JIA LI
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, 2011 Zonal Ave, Los Angeles, CA 90033 1054, USA
    J Virol 80:6478-86. 2006
  5. pmc Hepatitis C virus triggers mitochondrial permeability transition with production of reactive oxygen species, leading to DNA damage and STAT3 activation
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, 90033, USA
    J Virol 80:7199-207. 2006
  6. pmc Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes
    Keigo Machida
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    J Immunol 185:6985-98. 2010
  7. pmc Interactions between viral nonstructural proteins and host protein hVAP-33 mediate the formation of hepatitis C virus RNA replication complex on lipid raft
    Lu Gao
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Virol 78:3480-8. 2004
  8. pmc Pooled RNAi screen identifies ubiquitin ligase Itch as crucial for influenza A virus release from the endosome during virus entry
    Wen Chi Su
    Institute of Molecular Biology and National RNAi Core Facility, Academia Sinica, Taipei 11529, Taiwan
    Proc Natl Acad Sci U S A 110:17516-21. 2013
  9. pmc Association of hepatitis C virus replication complexes with microtubules and actin filaments is dependent on the interaction of NS3 and NS5A
    Chao Kuen Lai
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    J Virol 82:8838-48. 2008
  10. ncbi request reprint Identification of a ribavirin-resistant NS5B mutation of hepatitis C virus during ribavirin monotherapy
    Kung Chia Young
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90089 9094, USA
    Hepatology 38:869-78. 2003

Collaborators

Detail Information

Publications66

  1. pmc Hepatitis C virus translation preferentially depends on active RNA replication
    Helene Minyi Liu
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America
    PLoS ONE 7:e43600. 2012
    ..Our findings together indicate that the translation of HCV RNA is coupled to RNA replication and that the both processes may occur at the same subcellular membrane compartments, which we term the replicasome...
  2. pmc Transcription of subgenomic mRNA of hepatitis delta virus requires a modified hepatitis delta antigen that is distinct from antigenomic RNA synthesis
    Chung Hsin Tseng
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    J Virol 82:9409-16. 2008
    ..We propose that acetylation and deacetylation of HDAg may provide a molecular switch for the synthesis of the different HDV RNA species...
  3. ncbi request reprint Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation
    Chao Kuen Lai
    Institute of Molecular Biology, Academia Sinica, Taipei, 115, Taiwan
    Virology 370:295-309. 2008
    ..These results demonstrate that NS3/4A impairs the efficiency of DNA repair by interacting with ATM and renders the cells more sensitive to DNA damage. This effect may contribute to HCV oncogenesis...
  4. pmc RNA-templated replication of hepatitis delta virus: genomic and antigenomic RNAs associate with different nuclear bodies
    YI JIA LI
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, 2011 Zonal Ave, Los Angeles, CA 90033 1054, USA
    J Virol 80:6478-86. 2006
    ..These findings provide additional evidence that HDV RNA synthesis occurs in the Pol I and Pol II transcription machineries, thus extending the capability of the cellular DNA-dependent RNA polymerases to utilizing RNA as templates...
  5. pmc Hepatitis C virus triggers mitochondrial permeability transition with production of reactive oxygen species, leading to DNA damage and STAT3 activation
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, 90033, USA
    J Virol 80:7199-207. 2006
    ..These HCV studies thus identified ROS, along with the previously identified NO, as the primary inducers of DSBs and mitochondrial damage in HCV-infected cells...
  6. pmc Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes
    Keigo Machida
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    J Immunol 185:6985-98. 2010
    ..These effects may explain the oncogenicity and immunological perturbation of HCV infection...
  7. pmc Interactions between viral nonstructural proteins and host protein hVAP-33 mediate the formation of hepatitis C virus RNA replication complex on lipid raft
    Lu Gao
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Virol 78:3480-8. 2004
    ..These results indicate that protein-protein interactions among the various HCV NS proteins and hVAP-33 are important for the formation of HCV replication complex...
  8. pmc Pooled RNAi screen identifies ubiquitin ligase Itch as crucial for influenza A virus release from the endosome during virus entry
    Wen Chi Su
    Institute of Molecular Biology and National RNAi Core Facility, Academia Sinica, Taipei 11529, Taiwan
    Proc Natl Acad Sci U S A 110:17516-21. 2013
    ..These findings also highlight the feasibility of pooled RNAi screening for exploring the cellular cofactors of lytic viruses. ..
  9. pmc Association of hepatitis C virus replication complexes with microtubules and actin filaments is dependent on the interaction of NS3 and NS5A
    Chao Kuen Lai
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    J Virol 82:8838-48. 2008
    ....
  10. ncbi request reprint Identification of a ribavirin-resistant NS5B mutation of hepatitis C virus during ribavirin monotherapy
    Kung Chia Young
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90089 9094, USA
    Hepatology 38:869-78. 2003
    ..Furthermore, the selection of an RBV-resistant variant with a single amino acid substitution in NS5B suggested that RBV may directly interact with HCV RNA polymerase, thus interfering with its enzymatic activity...
  11. pmc Hepatitis C virus (HCV)-induced immunoglobulin hypermutation reduces the affinity and neutralizing activities of antibodies against HCV envelope protein
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    J Virol 82:6711-20. 2008
    ..These results suggest that HCV infection could cause some anti-HCV-antibody-producing hybridoma B cells to make less-protective antibodies...
  12. pmc c-Jun mediates hepatitis C virus hepatocarcinogenesis through signal transducer and activator of transcription 3 and nitric oxide-dependent impairment of oxidative DNA repair
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA
    Hepatology 52:480-92. 2010
    ....
  13. pmc The C-terminal transmembrane domain of hepatitis C virus (HCV) RNA polymerase is essential for HCV replication in vivo
    Ki Jeong Lee
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA
    J Virol 78:3797-802. 2004
    ..This finding suggests that the C-terminal 21 amino acids not only have a membrane-anchoring function but also may perform additional functions for RNA synthesis in vivo...
  14. ncbi request reprint Characterization of the hepatitis C virus RNA replication complex associated with lipid rafts
    Hideki Aizaki
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    Virology 324:450-61. 2004
    ..Depletion of cellular cholesterol selectively reduced HCV RNA replication. These findings provide further insights into the mechanism of HCV replication in vivo...
  15. ncbi request reprint Polypyrimidine-tract-binding protein is a component of the HCV RNA replication complex and necessary for RNA synthesis
    Hideki Aizaki
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Ave, HMR 503, Los Angeles, CA 90033, USA
    J Biomed Sci 13:469-80. 2006
    ..These studies together indicated that PTB is a part of the HCV RNA replication complex and participates in viral RNA synthesis. Thus, PTB has dual functions in HCV life cycle, including translation and RNA replication...
  16. pmc Hepatitis C virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon and interleukin-6
    Keigo Machida
    Department of Molecular Microbiology and Immunology, USC Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    J Virol 80:866-74. 2006
    ..In conclusion, HCV infection directly induces TLR4 expression and thereby activates B cells, which may contribute to the host's innate immune responses...
  17. pmc SYNCRIP (synaptotagmin-binding, cytoplasmic RNA-interacting protein) is a host factor involved in hepatitis C virus RNA replication
    Helene Minyi Liu
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA
    Virology 386:249-56. 2009
    ..These findings indicate that SYNCRIP has dual functions, participating in both RNA replication and translation in HCV life cycle...
  18. pmc Hepatitis C virus E2-CD81 interaction induces hypermutation of the immunoglobulin gene in B cells
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Ave, Los Angeles, California 90033, USA
    J Virol 79:8079-89. 2005
    ....
  19. pmc Modification of small hepatitis delta virus antigen by SUMO protein
    Chung Hsin Tseng
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    J Virol 84:918-27. 2010
    ..Sumoylation represents a new type of modification for HDAg...
  20. pmc Hepatitis C virus causes uncoupling of mitotic checkpoint and chromosomal polyploidy through the Rb pathway
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA
    J Virol 83:12590-600. 2009
    ..Taken together, these data suggest that HCV infection may inhibit the mitotic checkpoint to induce polyploidy, which likely contributes to neoplastic transformation...
  21. pmc Annexin A2 is involved in the formation of hepatitis C virus replication complex on the lipid raft
    Vikas Saxena
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    J Virol 86:4139-50. 2012
    ..Since Anxa2 is known to induce the formation of the lipid raft microdomain, we propose that Anxa2 recruits HCV NS proteins and enriches them on the lipid raft to form the HCV replication complex...
  22. ncbi request reprint Enzymatic characterization of the full-length and C-terminally truncated hepatitis C virus RNA polymerases: function of the last 21 amino acids of the C terminus in template binding and RNA synthesis
    Nam Viet Vo
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033 1054, USA
    Biochemistry 43:10579-91. 2004
    ..Together, these findings indicated that the HCV NS5B C(21) domain, in addition to being a membrane anchor, functions in template binding, NTP substrate selection, and modulation of terminal transferase activity...
  23. pmc Hepatitis C virus infection activates the immunologic (type II) isoform of nitric oxide synthase and thereby enhances DNA damage and mutations of cellular genes
    Keigo Machida
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, 2011 Zonal Ave, Los Angeles, CA 90033, USA
    J Virol 78:8835-43. 2004
    ..NO causes DNA breaks and enhances DNA mutation. This sequence of events provides a mechanism for HCV pathogenesis and oncogenesis...
  24. pmc Palmitoylation and polymerization of hepatitis C virus NS4B protein
    Guann Yi Yu
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA 90033 1054, USA
    J Virol 80:6013-23. 2006
    ..The lipid modification and the polymerization activity could be two properties of NS4B important for its induction of the specialized membrane structure involved in viral RNA replication...
  25. pmc Hepatitis C virus egress and release depend on endosomal trafficking of core protein
    Chao Kuen Lai
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    J Virol 84:11590-8. 2010
    ..We also observed that this way is independent of that of the internalization of endocytosed virus particles during virus entry...
  26. pmc Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy
    Wen Chi Su
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    J Virol 85:10561-71. 2011
    ..Taken together, these results not only reveal a novel role of NS4B in autophagy but also offer a clue to the mechanism of HCV-induced autophagy...
  27. pmc Proline-serine-threonine phosphatase-interacting protein 2 (PSTPIP2), a host membrane-deforming protein, is critical for membranous web formation in hepatitis C virus replication
    Ti Chun Chao
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    J Virol 86:1739-49. 2012
    ..Taking these results together, we suggest that PSTPIP2 facilitates membrane alterations and is a key player in the formation of the membranous web, which is the site of the HCV replication complex...
  28. pmc Hepatitis delta virus RNA encoding the large delta antigen cannot sustain replication due to rapid accumulation of mutations associated with RNA editing
    Thomas B Macnaughton
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033 1054, USA
    J Virol 77:12048-56. 2003
    ..They also explain why L-HDAg is not produced early in HDV infection, despite the fact that HDV-L RNA is present in the virion...
  29. pmc Large hepatitis delta antigen is not a suppressor of hepatitis delta virus RNA synthesis once RNA replication is established
    Thomas B Macnaughton
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, California 90033 1054, USA
    J Virol 76:9910-9. 2002
    ....
  30. pmc Hepatitis C virus RNA replication occurs on a detergent-resistant membrane that cofractionates with caveolin-2
    Stephanie T Shi
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Virol 77:4160-8. 2003
    ..We suggest that HCV RNA synthesis occurs on a lipid raft membrane structure...
  31. pmc Hepatitis C virus induces a mutator phenotype: enhanced mutations of immunoglobulin and protooncogenes
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    Proc Natl Acad Sci U S A 101:4262-7. 2004
    ..These results indicate that HCV induces a mutator phenotype and may transform cells by a hit-and-run mechanism. This finding provides a mechanism of oncogenesis for an RNA virus...
  32. pmc Murine coronavirus requires lipid rafts for virus entry and cell-cell fusion but not for virus release
    Keum S Choi
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, 90033 1054, USA
    J Virol 79:9862-71. 2005
    ..These findings suggest that MHV entry requires specific interactions between the spike protein and lipid rafts, probably during the virus internalization step...
  33. pmc Hepatitis delta virus antigen is methylated at arginine residues, and methylation regulates subcellular localization and RNA replication
    YI JIA LI
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, 2011 Zonal Ave, Los Angeles, CA 90033 1054, USA
    J Virol 78:13325-34. 2004
    ....
  34. pmc Transient activation of the PI3K-AKT pathway by hepatitis C virus to enhance viral entry
    Zhe Liu
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Biol Chem 287:41922-30. 2012
    ..These results provide important information for understanding HCV replication and pathogenesis and raised the possibility of targeting this cellular pathway to treat HCV patients...
  35. pmc Attenuation of 40S ribosomal subunit abundance differentially affects host and HCV translation and suppresses HCV replication
    Jing Ying Huang
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    PLoS Pathog 8:e1002766. 2012
    ....
  36. doi request reprint Hepatitis C virus infection of T cells inhibits proliferation and enhances fas-mediated apoptosis by down-regulating the expression of CD44 splicing variant 6
    Yasuteru Kondo
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, USA Division of Gastroenterology, Tohoku University, Sendai City
    J Infect Dis 199:726-36. 2009
    ..During T cell development and activation, transient expression of CD44 splicing variant 6 (CD44v6) plays a significant role...
  37. pmc SYNCRIP, a member of the heterogeneous nuclear ribonucleoprotein family, is involved in mouse hepatitis virus RNA synthesis
    Keum S Choi
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Ave, HMR 401, Los Angeles, CA 90033 1054, USA
    J Virol 78:13153-62. 2004
    ..These results suggest that SYNCRIP may be directly involved in MHV RNA replication as a positive regulator. This study identified an additional cellular hnRNP as an MHV RNA-binding protein potentially involved in viral RNA synthesis...
  38. pmc The SUMOylation of matrix protein M1 modulates the assembly and morphogenesis of influenza A virus
    Chung Yi Wu
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    J Virol 85:6618-28. 2011
    ..Therefore, we suggest that M1 can serve as a target for developing a new generation of drugs for flu therapy...
  39. ncbi request reprint Hepatitis C virus infects T cells and affects interferon-gamma signaling in T cell lines
    Yasuteru Kondo
    Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    Virology 361:161-73. 2007
    ..In conclusion, HCV could infect and transiently replicate in T cells and that HCV replication suppressed the IFN-gamma/STAT-1/T-bet signaling due to the reduction of STAT-1 and inhibition of its activation (phosphorylation)...
  40. ncbi request reprint Mutagenic and inhibitory effects of ribavirin on hepatitis C virus RNA polymerase
    Nam V Vo
    Department of Molecular Microbiology and Immunology, University of Southern California School of Medicine, Los Angeles, California 90033 1054, USA
    Biochemistry 42:10462-71. 2003
    ..Consequently, the level of RNA synthesis on a ribavirin-containing template is significantly reduced. These findings suggest that ribavirin not only is mutagenic but also interferes with HCV polymerase-mediated RNA synthesis...
  41. ncbi request reprint Reactive oxygen species suppress hepatitis C virus RNA replication in human hepatoma cells
    Jinah Choi
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA
    Hepatology 39:81-9. 2004
    ..In conclusion, our results show that ROS can rapidly inhibit HCV RNA replication in human hepatoma cells. The increased ROS levels in hepatitis C patients may therefore play an important role in the suppression of HCV replication...
  42. pmc Genomic but not antigenomic hepatitis delta virus RNA is preferentially exported from the nucleus immediately after synthesis and processing
    Thomas B Macnaughton
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033 1054, USA
    J Virol 76:3928-35. 2002
    ..These findings uncover a previously unrecognized presence of HDV RNA in the cytoplasm, which may have implications for viral RNA synthesis and packaging...
  43. ncbi request reprint Polypyrimidine-tract-binding protein affects transcription but not translation of mouse hepatitis virus RNA
    Keum S Choi
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA
    Virology 303:58-68. 2002
    ....
  44. pmc Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatic oncogenesis involving stem cell marker Nanog
    Keigo Machida
    Southern California Research Center for Alcoholic, Liver, and Pancreatic Diseases and Cirrhosis, and Department of Molecular Microbiology, University of Southern California, Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    Proc Natl Acad Sci U S A 106:1548-53. 2009
    ..Taken together, our study demonstrates a TLR4-dependent mechanism of synergistic liver disease by HCV and alcohol and an obligatory role for Nanog, a TLR4 downstream gene, in HCV-induced liver oncogenesis enhanced by alcohol...
  45. pmc Poly(C)-binding protein 2 interacts with sequences required for viral replication in the hepatitis C virus (HCV) 5' untranslated region and directs HCV RNA replication through circularizing the viral genome
    Linya Wang
    Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    J Virol 85:7954-64. 2011
    ..This study thus demonstrates the mechanism of the participation of PCBP2 in HCV translation and replication and provides physical evidence for HCV RNA circularization through 5'- and 3'UTR interaction...
  46. pmc Ubiquitination and deubiquitination of NP protein regulates influenza A virus RNA replication
    Tsai Ling Liao
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    EMBO J 29:3879-90. 2010
    ..Finally, we identified K184 as the ubiquitination site on NP and this residue is crucial for virus RNA replication. We propose that ubiquitination/deubiquitination of NP can be manipulated for antiviral therapeutic purposes...
  47. pmc Interaction with a ubiquitin-like protein enhances the ubiquitination and degradation of hepatitis C virus RNA-dependent RNA polymerase
    Lu Gao
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033 1054, USA
    J Virol 77:4149-59. 2003
    ..Thus, hPLIC1 may be a regulator of HCV RNA replication through interaction with NS5B...
  48. pmc Establishment of B-cell lymphoma cell lines persistently infected with hepatitis C virus in vivo and in vitro: the apoptotic effects of virus infection
    Vicky M H Sung
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Virol 77:2134-46. 2003
    ..HCV-infected cell lines show significantly enhanced apoptosis. These B-cell lines provide a reproducible cell culture system for studying the complete replication cycle and biology of HCV infections...
  49. doi request reprint The 8ab protein of SARS-CoV is a luminal ER membrane-associated protein and induces the activation of ATF6
    Shu Chiun Sung
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    Virology 387:402-13. 2009
    ..These findings suggest that 8ab could modulate the UPR by activating ATF6 to facilitate protein folding and processing. Thus, the loss of 8ab in SARS-CoV through viral evolution in animals may play a role in its pathogenicity...
  50. pmc The ubiquitin-proteasome system facilitates the transfer of murine coronavirus from endosome to cytoplasm during virus entry
    Guann Yi Yu
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033 1054, USA
    J Virol 79:644-8. 2005
    ....
  51. pmc RNA replication without RNA-dependent RNA polymerase: surprises from hepatitis delta virus
    Michael M C Lai
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, 2011 Zonal Ave, HMR503C, Los Angeles, California 90033, USA
    J Virol 79:7951-8. 2005
  52. pmc Rolling circle replication of hepatitis delta virus RNA is carried out by two different cellular RNA polymerases
    Thomas B Macnaughton
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033 1054, USA
    J Virol 76:3920-7. 2002
    ....
  53. ncbi request reprint Hepatitis delta virus RNA transfection for the cell culture model
    Thomas B Macnaughton
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, USA
    Methods Mol Med 96:351-7. 2004
  54. ncbi request reprint Identification of RNA ligands that bind hepatitis C virus polymerase selectively and inhibit its RNA synthesis from the natural viral RNA templates
    Nam Viet Vo
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033 1054, USA
    Virology 307:301-16. 2003
    ..These RNA ligands will be useful for further characterization of NS5B-binding properties and, with further modifications, may have potential therapeutic value...
  55. ncbi request reprint Detection and characterization of small and large HDV antigens
    Thomas B Macnaughton
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, USA
    Methods Mol Med 95:99-105. 2004
  56. ncbi request reprint Hepatitis C virus IRES-dependent translation is insensitive to an eIF2alpha-independent mechanism of inhibition by interferon in hepatocyte cell lines
    Gennadiy Koev
    Howard Hughes Medical Institute, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Virology 297:195-202. 2002
    ..This may present a new potential mechanism of viral resistance to IFN treatment during the early steps of virus infection...
  57. ncbi request reprint The hepatitis C virus persistence: how to evade the immune system?
    Nicole Pavio
    Department of Molecular Microbiology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA
    J Biosci 28:287-304. 2003
    ..These various strategies used by HCV to counter the immune response of the host are reviewed here. A better understanding of these mechanisms would help design new therapeutic targets...
  58. pmc Hepatitis delta virus RNA replication
    Chung Hsin Tseng
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan
    Viruses 1:818-31. 2009
    ..Here the HDV RNA replication cycle is reviewed, with emphasis on the function of HDAg in modulating RNA replication and the nature of the enzyme involved...
  59. pmc Multiple type A/B heterogeneous nuclear ribonucleoproteins (hnRNPs) can replace hnRNP A1 in mouse hepatitis virus RNA synthesis
    Stephanie T Shi
    Department of Molecular Microbiology and Immunology and Howard Hughes Medical Institute, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Virol 77:10584-93. 2003
    ..Our findings demonstrate that the functions of hnRNP A1 in MHV RNA synthesis can be replaced by other closely related hnRNPs, further supporting the roles of cellular proteins in MHV RNA synthesis...
  60. ncbi request reprint Treatment of HCV-related mantle-cell lymphoma with ribavirin and pegylated interferon Alfa
    Alexandra M Levine
    N Engl J Med 349:2078-9. 2003
  61. ncbi request reprint Nonstructural 5A protein of hepatitis C virus modulates tumor necrosis factor alpha-stimulated nuclear factor kappa B activation
    Kyu Jin Park
    Institute of Environment and Life Science, The Hallym Academy of Sciences, Hallym University, 1 Ockcheon dong, Chuncheon 200 702, Korea
    J Biol Chem 277:13122-8. 2002
    ..Our findings suggest a possible molecular mechanism that could explain the ability of NS5A to negatively regulate TNF-alpha-induced NF-kappaB activation...
  62. pmc Hepatitis C virus nonstructural 5B protein regulates tumor necrosis factor alpha signaling through effects on cellular IkappaB kinase
    Soo Ho Choi
    Ilsong Institute of Life Science, Hallym University, 1 Ockcheon dong, Chuncheon 200 702, South Korea
    Mol Cell Biol 26:3048-59. 2006
    ..These data suggest that NS5B protein modulates TNF-alpha signaling pathways and may contribute to HCV pathogenesis...
  63. pmc Critical role of virion-associated cholesterol and sphingolipid in hepatitis C virus infection
    Hideki Aizaki
    Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Virol 82:5715-24. 2008
    ....
  64. ncbi request reprint [Hepatitis C virus replication associated with lipid rafts]
    Hideki Aizaki
    Department of Virology II, National Institute of Infectious Diseases, Shinjuku ku, Tokyo 162 8640, Japan
    Seikagaku 78:321-6. 2006
  65. ncbi request reprint Molecular pathogenesis of Gilbert's syndrome: decreased TATA-binding protein binding affinity of UGT1A1 gene promoter
    Tsai Yuan Hsieh
    Department of Internal Medicine, Tri Service General Hospital, Taipei, Taiwan, ROC
    Pharmacogenet Genomics 17:229-36. 2007
    ..The mechanism responsible for this decrease in UGT1A1 activity, however, has not been elucidated. To clarify the mechanism underlying this deficiency in UGT1A1 activity in patients with Gilbert's syndrome...
  66. ncbi request reprint Redox modulation of the hepatitis C virus replication complex is calcium dependent
    Jinah Choi
    School of Natural Sciences, University of California at Merced, CA 95344, USA
    Free Radic Biol Med 41:1488-98. 2006
    ..Effects on the infectivity and the morphogenesis of HCV remain to be determined. These findings suggest possible regulatory roles for redox and calcium signaling during viral infections...