Live cell imaging with protein domains capable of recognizing phosphatidylinositol 4,5-bisphosphate; a comparative studyZsofia Szentpetery
Sections on molecular signal transduction, Program for Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
BMC Cell Biol 10:67. 2009
..Here we report on a comparative analysis of several recently-described yeast PH domains as well as the mammalian Tubby domain to evaluate their usefulness as PtdIns(4,5)P2 imaging tools...
- Dependence of STIM1/Orai1-mediated calcium entry on plasma membrane phosphoinositides
Marek K Korzeniowski
Sections on molecular signal transduction, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 284:21027-35. 2009
..These results suggest an inositide requirement of Orai1 activation but not STIM1 movements and indicate that PtdIns4P rather than PtdIns(4,5)P2 is a likely determinant of Orai1 channel activity...
- A PH domain in the Arf GTPase-activating protein (GAP) ARAP1 binds phosphatidylinositol 3,4,5-trisphosphate and regulates Arf GAP activity independently of recruitment to the plasma membranes
Fanny Campa
Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 284:28069-83. 2009
..In our model, ARAP1 is recruited to membranes independently of PtdIns(3,4,5)P(3), the subsequent production of which triggers enzymatic activity...
- Two phosphatidylinositol 4-kinases control lysosomal delivery of the Gaucher disease enzyme, β-glucocerebrosidase
Marko Jovic
Section on Molecular Signal Transduction, Program for Developmental Neuroscience, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 23:1533-45. 2012
..These studies identified PI4KIIIβ and PI4KIIα as important regulators of lysosomal delivery of GBA, revealing a new element of control to sphingolipid homeostasis by phosphoinositides...
- Acute manipulation of Golgi phosphoinositides to assess their importance in cellular trafficking and signaling
Zsofia Szentpetery
Section on Molecular Signal Transduction, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:8225-30. 2010
..This unique approach will allow further studies on the role of phosphoinositides in endocytic compartments that have evaded detection using the conventional long-term manipulations of inositide kinase and phosphatase activities...
- Maintenance of hormone-sensitive phosphoinositide pools in the plasma membrane requires phosphatidylinositol 4-kinase IIIalpha
Andras Balla
Section on Molecular Signal Transduction, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 19:711-21. 2008
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- Phosphoinositide signaling: new tools and insights
Tamas Balla
Section on Molecular Signal Transduction, Program for Developmental Neuroscience, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
Physiology (Bethesda) 24:231-44. 2009
..The recent progress allows us to understand the underlying causes of certain human diseases and design new strategies for therapeutic interventions...
Design of drug-resistant alleles of type-III phosphatidylinositol 4-kinases using mutagenesis and molecular modelingAndras Balla
Section on Molecular Signal Transduction, Center for Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
Biochemistry 47:1599-607. 2008
..These studies should aid development of subtype-specific inhibitors of type-III PI4Ks and help to better understand the significance of localized PtdIns4P production by the various PI4Ks isoforms in specific cellular compartments...
- Genetic and functional studies of phosphatidyl-inositol 4-kinase type IIIα
Zsofia Szentpetery
Program for Developmental Neuroscience, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
Biochim Biophys Acta 1811:476-83. 2011
..These data draw attention to PI4KIIIα as one of the genes found in Chr22q11, a region affected by chromosomal instability, but do not substantiate the existence of a functionally relevant short form of PI4KIIIα...
