Igor Stagljar

Summary

Affiliation: University of Zurich
Country: Switzerland

Publications

  1. ncbi request reprint Finding partners: emerging protein interaction technologies applied to signaling networks
    Igor Stagljar
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Sci STKE 2003:pe56. 2003
  2. ncbi request reprint Analysis of membrane protein interactions using yeast-based technologies
    Igor Stagljar
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, Switzerland
    Trends Biochem Sci 27:559-63. 2002
  3. ncbi request reprint Utilizing the split-ubiquitin membrane yeast two-hybrid system to identify protein-protein interactions of integral membrane proteins
    Kavitha Iyer
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, CH 8057 Zurich, Switzerland
    Sci STKE 2005:pl3. 2005
  4. ncbi request reprint Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions
    Michael Fetchko
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Methods 32:349-62. 2004
  5. pmc Physical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1
    Renjie Jiao
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurstr 190, CH 8057 Zurich, Switzerland
    Mol Cell Biol 24:4710-9. 2004
  6. ncbi request reprint The human Rothmund-Thomson syndrome gene product, RECQL4, localizes to distinct nuclear foci that coincide with proteins involved in the maintenance of genome stability
    Maja Petkovic
    Institute of Vet Biochemistry and Molecular Biology, University of Zurich, Winterthurerstr 190, 8057 Zurich, Switzerland
    J Cell Sci 118:4261-9. 2005
  7. pmc The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase delta
    Nives Selak
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    Nucleic Acids Res 36:5166-79. 2008
  8. pmc Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system
    Safia Thaminy
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, CH 8057 Zurich, Switzerland
    Genome Res 13:1744-53. 2003
  9. ncbi request reprint Yeast genetic methods for the detection of membrane protein interactions: potential use in drug discovery
    Michael Fetchko
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Zurich, Switzerland
    BioDrugs 17:413-24. 2003
  10. pmc Monitoring protein-protein interactions between the mammalian integral membrane transporters and PDZ-interacting partners using a modified split-ubiquitin membrane yeast two-hybrid system
    Serge M Gisler
    Institute of Physiology and Center for Integrative Human Physiology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Mol Cell Proteomics 7:1362-77. 2008

Collaborators

  • Ian D Hickson
  • Ulrich Hubscher
  • Jurg Biber
  • Josef Jiricny
  • Raimundo Freire
  • Ji Liang Li
  • Daniel Markovich
  • Pavel Janscak
  • Asa Ben-Hur
  • Karin Römisch
  • H Murer
  • W Scheper
  • Peter Uetz
  • Bernhard Suter
  • Daniel Auerbach
  • Safia Thaminy
  • Christian M Paumi
  • Graziella Pedrazzi
  • Anthony Arnoldo
  • Maja Petkovic
  • Saranya Kittanakom
  • Michael Fetchko
  • Jeffrey Fillingham
  • Tobias Dietschy
  • Nives Selak
  • Csanad Z Bachrati
  • Renjie Jiao
  • John Miller
  • Serge M Gisler
  • Igor Chevelev
  • Guri Giaever
  • Jack F Greenblatt
  • Igor Shevelev
  • Corey Nislow
  • Susan Michaelis
  • Natalie Möckli
  • Nurten Saydam
  • Kim Engels
  • Kavitha Iyer
  • John P Miller
  • Michael O Hottiger
  • Anke Reinders
  • Vincent T Lee
  • Stephen Lory
  • Orson W Moe
  • Peter J Roy
  • Tamara Radanovic
  • Antonio Bedalov
  • Randy A Hall
  • C David Allis
  • Barbara van Loon
  • Matthew Chuk
  • Anette Jacob
  • Andrew Emili
  • Nevan J Krogan
  • A Rod Merrill
  • Andrea C Silva
  • Rod A Merrill
  • Mehdi Sahebol-Amri
  • Mia Bertic
  • Victoria Wong
  • Jasna Curak
  • Lana Ljuma
  • Judith Recht
  • Michael Christopher Keogh
  • Joerg D Hoheisel
  • Becky Koscik
  • Daniel Fuster
  • Caroline Zbinden
  • Matthias Peter
  • Lucija Beneti
  • Paul O Hassa
  • Lianet Lopez
  • Jacqueline Hort
  • Patrick L Garcia
  • Javier Menendez
  • Oleg Georgiev
  • Michael J Fetchko
  • Javier Pena-Diaz
  • Ralph Imhof
  • Zhaolei Zhang
  • Ingrid Stoffel-Studer
  • Anna Deplazes
  • Radhakrishnan Kanagaraj
  • Yves Barral
  • Maanasa Raghavan
  • Christopher I Graham
  • Kavitha Ravee Iyer
  • William Stafford Noble
  • Stanley Fields

Detail Information

Publications28

  1. ncbi request reprint Finding partners: emerging protein interaction technologies applied to signaling networks
    Igor Stagljar
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Sci STKE 2003:pe56. 2003
    ..Although still at an early stage, these technologies show promise as useful methods for the characterization of novel components of various signal transduction pathways...
  2. ncbi request reprint Analysis of membrane protein interactions using yeast-based technologies
    Igor Stagljar
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, Switzerland
    Trends Biochem Sci 27:559-63. 2002
    ..We summarize here recent yeast-based interaction technologies that can be applied to membrane proteins...
  3. ncbi request reprint Utilizing the split-ubiquitin membrane yeast two-hybrid system to identify protein-protein interactions of integral membrane proteins
    Kavitha Iyer
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, CH 8057 Zurich, Switzerland
    Sci STKE 2005:pl3. 2005
    ..We also describe how this technology is used to screen complementary DNA libraries to identify novel binding partners of a membrane protein of interest...
  4. ncbi request reprint Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions
    Michael Fetchko
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Methods 32:349-62. 2004
    ..Herein, we provide a description of the MbYTH method and detailed protocols in order to monitor protein interactions and discover novel interacting partners using the MbYTH system...
  5. pmc Physical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1
    Renjie Jiao
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurstr 190, CH 8057 Zurich, Switzerland
    Mol Cell Biol 24:4710-9. 2004
    ..Our results provide the first link between BLM and chromatin assembly coupled to DNA repair and suggest that BLM and CAF-1 function in a coordinated way to promote survival in response to DNA damage and/or replication blockade...
  6. ncbi request reprint The human Rothmund-Thomson syndrome gene product, RECQL4, localizes to distinct nuclear foci that coincide with proteins involved in the maintenance of genome stability
    Maja Petkovic
    Institute of Vet Biochemistry and Molecular Biology, University of Zurich, Winterthurerstr 190, 8057 Zurich, Switzerland
    J Cell Sci 118:4261-9. 2005
    ..Our findings suggest a role for RECQL4 in the repair of DNA double-strand breaks by homologous recombination and shed new light onto RECQL4's function in human cells...
  7. pmc The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase delta
    Nives Selak
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    Nucleic Acids Res 36:5166-79. 2008
    ..Finally, our data also define a novel role for the poorly characterized p12 subunit of hPOL delta...
  8. pmc Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system
    Safia Thaminy
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, CH 8057 Zurich, Switzerland
    Genome Res 13:1744-53. 2003
    ..We expect the split-ubiquitin membrane YTH technology to be valuable for the identification of potential interacting partners of integral membrane proteins from many model organisms...
  9. ncbi request reprint Yeast genetic methods for the detection of membrane protein interactions: potential use in drug discovery
    Michael Fetchko
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Zurich, Switzerland
    BioDrugs 17:413-24. 2003
    ..In this review, we speculate on the feasibility of using inhibitors of protein interactions as drugs and the adaptation of these techniques to select for inhibitors of defined protein interactions...
  10. pmc Monitoring protein-protein interactions between the mammalian integral membrane transporters and PDZ-interacting partners using a modified split-ubiquitin membrane yeast two-hybrid system
    Serge M Gisler
    Institute of Physiology and Center for Integrative Human Physiology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Mol Cell Proteomics 7:1362-77. 2008
    ..In summary, our study establishes the MYTH 2.0 system as a novel tool for interactive proteomics studies of membrane protein complexes...
  11. ncbi request reprint Protein-protein interactions
    Graziella Pedrazzi
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Switzerland
    Methods Mol Biol 241:269-83. 2004
  12. ncbi request reprint Genetic approaches to the identification of interactions between membrane proteins in yeast
    Daniel Auerbach
    DUALSYSTEMS BIOTECH Inc, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Recept Signal Transduct Res 22:471-81. 2002
    ....
  13. ncbi request reprint The Bloom's syndrome helicase interacts directly with the human DNA mismatch repair protein hMSH6
    Graziella Pedrazzi
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    Biol Chem 384:1155-64. 2003
    ....
  14. pmc Large-scale identification of yeast integral membrane protein interactions
    John P Miller
    Department of Genome Sciences, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 102:12123-8. 2005
    ..The results identify potential previously undescribed components of established biological processes and roles for integral membrane proteins of ascribed functions...
  15. ncbi request reprint The split-ubiquitin membrane-based yeast two-hybrid system
    Safia Thaminy
    Institut of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Zurich, Switzerland
    Methods Mol Biol 261:297-312. 2004
    ....
  16. pmc Negative regulation of the yeast ABC transporter Ycf1p by phosphorylation within its N-terminal extension
    Christian M Paumi
    Department of Cell Biology, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 283:27079-88. 2008
    ..Overall, this study provides compelling evidence that negative, as well as positive, regulation of Ycf1p is mediated by phosphorylation...
  17. doi request reprint Interactive proteomics: what lies ahead?
    Bernhard Suter
    Department of Biochemistry and Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research dCCBR, University of Toronto, Toronto, ON, Canada
    Biotechniques 44:681-91. 2008
    ..In this review, we present an impressive toolbox of different technologies that we expect to be crucial for interactive proteomics in the coming years...
  18. pmc Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109
    Jeffrey Fillingham
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research CCBR, University of Toronto, 160 College St, Toronto, Ontario M5S 3E1, Canada
    Mol Cell Biol 28:4342-53. 2008
    ..Asf1 also physically interacts with the nuclear Hat1/Hat2/Hif1 complex that acetylates H4-K5 and H4-K12. We suggest Asf1 is capable of assembling into chromatin H3-H4 dimers diacetylated on both H4-K5/12 and H3-K9/56...
  19. pmc Identification of small molecule inhibitors of Pseudomonas aeruginosa exoenzyme S using a yeast phenotypic screen
    Anthony Arnoldo
    Terrence Donnelly Centre for Cellular and Biomolecular Research, Department of Biochemistry, University of Toronto, Ontario, Canada
    PLoS Genet 4:e1000005. 2008
    ..Taken together, our integrated cell-based, chemical-genetic approach demonstrates that such screens can augment traditional drug screening approaches and facilitate the discovery of new compounds against a broad range of human pathogens...
  20. ncbi request reprint Coordination of N-glycosylation and protein translocation across the endoplasmic reticulum membrane by Sss1 protein
    Wiep Scheper
    University of Cambridge, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:37998-8003. 2003
    ..Our data suggest that Sss1p coordinates translocation across the ER membrane and N-linked glycosylation of secretory proteins...
  21. pmc Examining protein protein interactions using endogenously tagged yeast arrays: the cross-and-capture system
    Bernhard Suter
    Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 3E1, Canada
    Genome Res 17:1774-82. 2007
    ..Our studies establish the "Cross-and-Capture" assay as a novel, versatile tool that provides a valuable complement for the next generation of yeast proteomic studies...
  22. pmc Physical and functional interactions between Werner syndrome helicase and mismatch-repair initiation factors
    Nurten Saydam
    Institute of Molecular Cancer Research of the University of Zurich, Switzerland
    Nucleic Acids Res 35:5706-16. 2007
    ..Our data are consistent with results of genetic experiments in yeast suggesting that MMR factors act in conjunction with a RecQ-type helicase to reject recombination between divergent sequences...
  23. ncbi request reprint Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins
    Natalie Möckli
    Dualsystems Biotech AG, Zurich, Switzerland
    Biotechniques 42:725-30. 2007
    ..The cytoY2H system extends existing methods for the detection of protein interactions by providing a convenient solution for screening a wide range of transcriptionally active proteins...
  24. ncbi request reprint Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis
    Christian M Paumi
    Department of Cell Biology, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Cell 26:15-25. 2007
    ..Our study provides proof of principle that iMYTH is an ideal methodology to identify physiological interactors and regulators of ABC transporters and other yeast transmembrane proteins...
  25. pmc The interactome of human EGF/ErbB receptors
    Peter Uetz
    Mol Syst Biol 2:2006.0006. 2006
  26. ncbi request reprint Yeast-based functional genomics and proteomics technologies: the first 15 years and beyond
    Bernhard Suter
    University of Toronto, Toronto, ON, Canada
    Biotechniques 40:625-44. 2006
    ..The integration of these advances with established technologies is invaluable in the drive toward a comprehensive understanding of protein structure and function in the cellular milieu...
  27. ncbi request reprint Using the yeast two-hybrid system to identify interacting proteins
    John Miller
    Department of Genome Sciences, University of Washington, Seattle, WA, USA
    Methods Mol Biol 261:247-62. 2004
    ..It is this latter feature-the ability to search for interacting proteins without any prior knowledge of the identity of such proteins-that is the most powerful application of the two-hybrid technique...
  28. ncbi request reprint Intra- and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays
    Anke Reinders
    Plant Biology, University of Minnesota, 220 Biological Sciences Center, 1445 Gortner Avenue, St Paul, MN 55108, USA
    Structure 10:763-72. 2002
    ..The split-ubiquitin system efficiently detects intramolecular interactions in membrane proteins, and can be used to dissect transporter structure...