Roger Schneiter

Summary

Affiliation: University of Fribourg
Country: Switzerland

Publications

  1. pmc Integrating complex functions: coordination of nuclear pore complex assembly and membrane expansion of the nuclear envelope requires a family of integral membrane proteins
    Roger Schneiter
    Division of Biochemistry, Department of Medicine, University of Fribourg, Fribourg, Switzerland
    Nucleus 1:387-92. 2010
  2. ncbi request reprint The role of lipids in the biogenesis of integral membrane proteins
    Roger Schneiter
    Department of Medicine, Division of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700, Fribourg, Switzerland
    Appl Microbiol Biotechnol 73:1224-32. 2007
  3. ncbi request reprint Intracellular sterol transport in eukaryotes, a connection to mitochondrial function?
    Roger Schneiter
    Department of Medicine, Division of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    Biochimie 89:255-9. 2007
  4. pmc Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes
    Roger Schneiter
    Division of Biochemistry, Department of Medicine, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    Biochem J 381:941-9. 2004
  5. ncbi request reprint Very long-chain fatty acid-containing lipids rather than sphingolipids per se are required for raft association and stable surface transport of newly synthesized plasma membrane ATPase in yeast
    Barbara Gaigg
    Division of Biochemistry, University of Fribourg, CH 1700 Fribourg, Switzerland
    J Biol Chem 281:34135-45. 2006
  6. pmc An acetylation/deacetylation cycle controls the export of sterols and steroids from S. cerevisiae
    Rashi Tiwari
    Department of Medicine, Division of Biochemistry, Institute of Biochemistry, University of Fribourg, Fribourg, Switzerland
    EMBO J 26:5109-19. 2007
  7. doi request reprint Lipid droplets are functionally connected to the endoplasmic reticulum in Saccharomyces cerevisiae
    Nicolas Jacquier
    Department of Biology, Division of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland
    J Cell Sci 124:2424-37. 2011
  8. doi request reprint Mechanisms of sterol uptake and transport in yeast
    Nicolas Jacquier
    Department of Medicine, Division of Biochemistry, University of Fribourg, Fribourg, Switzerland
    J Steroid Biochem Mol Biol 129:70-8. 2012
  9. pmc Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins
    Vineet Choudhary
    Division of Biochemistry, Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland
    Proc Natl Acad Sci U S A 109:16882-7. 2012
  10. doi request reprint Monitoring sterol uptake, acetylation, and export in yeast
    Vineet Choudhary
    Department of Medicine, Division of Biochemistry, University of Fribourg, Fribourg, Switzerland
    Methods Mol Biol 580:221-32. 2009

Collaborators

Detail Information

Publications30

  1. pmc Integrating complex functions: coordination of nuclear pore complex assembly and membrane expansion of the nuclear envelope requires a family of integral membrane proteins
    Roger Schneiter
    Division of Biochemistry, Department of Medicine, University of Fribourg, Fribourg, Switzerland
    Nucleus 1:387-92. 2010
    ....
  2. ncbi request reprint The role of lipids in the biogenesis of integral membrane proteins
    Roger Schneiter
    Department of Medicine, Division of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700, Fribourg, Switzerland
    Appl Microbiol Biotechnol 73:1224-32. 2007
    ..Here, we review and discuss some examples that illustrate the interdependence between bilayer lipids and the biogenesis of integral membrane proteins...
  3. ncbi request reprint Intracellular sterol transport in eukaryotes, a connection to mitochondrial function?
    Roger Schneiter
    Department of Medicine, Division of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    Biochimie 89:255-9. 2007
    ....
  4. pmc Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes
    Roger Schneiter
    Division of Biochemistry, Department of Medicine, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    Biochem J 381:941-9. 2004
    ..The biophysical properties of this lipid are thus consistent with a possible role in stabilizing highly curved membrane domains...
  5. ncbi request reprint Very long-chain fatty acid-containing lipids rather than sphingolipids per se are required for raft association and stable surface transport of newly synthesized plasma membrane ATPase in yeast
    Barbara Gaigg
    Division of Biochemistry, University of Fribourg, CH 1700 Fribourg, Switzerland
    J Biol Chem 281:34135-45. 2006
    ..We thus conclude that the essential function of sphingolipids for membrane domain formation and stable surface delivery of Pma1p is provided by the C26 fatty acid that forms part of the yeast ceramide...
  6. pmc An acetylation/deacetylation cycle controls the export of sterols and steroids from S. cerevisiae
    Rashi Tiwari
    Department of Medicine, Division of Biochemistry, Institute of Biochemistry, University of Fribourg, Fribourg, Switzerland
    EMBO J 26:5109-19. 2007
    ..The fact that homologues of SAY1 are present in the mammalian genome and functionally substitute for SAY1 in yeast indicates that part of this pathway has been evolutionarily conserved...
  7. doi request reprint Lipid droplets are functionally connected to the endoplasmic reticulum in Saccharomyces cerevisiae
    Nicolas Jacquier
    Department of Biology, Division of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland
    J Cell Sci 124:2424-37. 2011
    ..These observations indicate that LDs are functionally connected to the ER membrane and that this connection allows the efficient partitioning of membrane proteins between the two compartments...
  8. doi request reprint Mechanisms of sterol uptake and transport in yeast
    Nicolas Jacquier
    Department of Medicine, Division of Biochemistry, University of Fribourg, Fribourg, Switzerland
    J Steroid Biochem Mol Biol 129:70-8. 2012
    ..Further analysis of this lipid quality control cycle is likely to reveal novel insight into the mechanisms that ensure sterol homeostasis in eukaryotic cells. Article from a special issue on Steroids and Microorganisms...
  9. pmc Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins
    Vineet Choudhary
    Division of Biochemistry, Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland
    Proc Natl Acad Sci U S A 109:16882-7. 2012
    ....
  10. doi request reprint Monitoring sterol uptake, acetylation, and export in yeast
    Vineet Choudhary
    Department of Medicine, Division of Biochemistry, University of Fribourg, Fribourg, Switzerland
    Methods Mol Biol 580:221-32. 2009
    ..The time-dependent appearance of acetylated sterols in the culture supernatant thus provides a new means to monitor the forward transport of chemically modified sterols out of the ER...
  11. ncbi request reprint Lipid-dependent surface transport of the proton pumping ATPase: a model to study plasma membrane biogenesis in yeast
    Alexandre Toulmay
    Department of Medicine, Division of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    Biochimie 89:249-54. 2007
    ..The resulting protein-lipid complex is then transported and sorted as an entity to the plasma membrane. Failure to successfully assemble this lipid-protein complex results in mistargeting of the protein to the vacuole...
  12. ncbi request reprint Ypk1, the yeast orthologue of the human serum- and glucocorticoid-induced kinase, is required for efficient uptake of fatty acids
    Nicolas Jacquier
    Department of Medicine, Division of Biochemistry, University of Fribourg, Chemin du Musee 5, 1700 Fribourg, Switzerland
    J Cell Sci 123:2218-27. 2010
    ..Consistent with such a proposition, mutations that block early steps of endocytosis display reduced levels of fatty-acid uptake...
  13. pmc Yeh1 constitutes the major steryl ester hydrolase under heme-deficient conditions in Saccharomyces cerevisiae
    René Köffel
    Department of Medicine, Division of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    Eukaryot Cell 5:1018-25. 2006
    ..These data thus provide the first link between fat degradation and the transcriptional control of lipase activity in yeast...
  14. ncbi request reprint Synthesis of sphingolipids with very long chain fatty acids but not ergosterol is required for routing of newly synthesized plasma membrane ATPase to the cell surface of yeast
    Barbara Gaigg
    Division of Biochemistry, University of Fribourg, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    J Biol Chem 280:22515-22. 2005
    ..Mistargeting correlates with a lack of newly synthesized Pma1p to acquire detergent resistance, suggesting that sphingolipids with very long acyl chains affect sorting of Pma1p to the cell surface...
  15. ncbi request reprint Expression of oleosin and perilipins in yeast promotes formation of lipid droplets from the endoplasmic reticulum
    Nicolas Jacquier
    Department of Biology, Division of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland
    J Cell Sci 126:5198-209. 2013
    ..Consistent with this proposition, addition of a cell-permeable diacylglycerol (DAG) was sufficient to promote LD formation in cells expressing the LD scaffolding proteins but lacking the capacity to synthesize storage lipids. ..
  16. ncbi request reprint Analysis of yeast lipids
    Roger Schneiter
    Division of Biochemistry, University of Fribourg, Switzerland
    Methods Mol Biol 313:75-84. 2006
    ..In this chapter, we describe methods based on thin-layer chromatography that are employed routinely to determine the level and relative composition of the major lipid classes from yeast...
  17. pmc The Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and TGL1 genes encode a novel family of membrane-anchored lipases that are required for steryl ester hydrolysis
    René Köffel
    University of Fribourg, Department of Medicine, Division of Biochemistry, Chemin du Musee 5, CH 1700 Fribourg, Switzerland
    Mol Cell Biol 25:1655-68. 2005
    ..The three yeast enzymes constitute a novel class of membrane-anchored lipases that differ in topology and subcellular localization...
  18. ncbi request reprint A two-step method for the introduction of single or multiple defined point mutations into the genome of Saccharomyces cerevisiae
    Alexandre Toulmay
    Department of Medicine, Division of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland
    Yeast 23:825-31. 2006
    ..It confers a temperature-sensitive growth phenotype and the newly synthesized Pma1-7 protein is unstable and rapidly degraded...
  19. pmc The topology of the triacylglycerol synthesizing enzyme Lro1 indicates that neutral lipids can be produced within the luminal compartment of the endoplasmatic reticulum: Implications for the biogenesis of lipid droplets
    Vineet Choudhary
    Department of Biology, Division of Biochemistry, University of Fribourg Fribourg, Switzerland
    Commun Integr Biol 4:781-4. 2011
    ..Since TAG formed by either Dga1 or Lro1 can be efficiently packed into LDs we conclude that neutral lipids from both the cytosolic as well as the luminal leaflets of the ER membrane can be concentrated and packed into LDs...
  20. ncbi request reprint Extraction of yeast lipids
    Roger Schneiter
    Division of Biochemistry, University of Fribourg, Switzerland
    Methods Mol Biol 313:41-5. 2006
    ..In this chapter, we describe a simple and rapid protocol that relies on glass bead disruption in the presence of organic solvents to quantitatively extract lipids from yeast cells...
  21. ncbi request reprint The caveolin-binding motif of the pathogen-related yeast protein Pry1, a member of the CAP protein superfamily, is required for in vivo export of cholesteryl acetate
    Vineet Choudhary
    Division of Biochemistry, Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland
    J Lipid Res 55:883-94. 2014
    ..These results indicate that the caveolin-binding motif of Pry1, and possibly of other CAP family members, is crucial for selective lipid binding and that lipid binding may occur through displacement of the loop containing this motif...
  22. pmc Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae
    Nils J Faergeman
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Biochem J 380:907-18. 2004
    ..We suggest that the reduced ceramide synthesis in Acb1p-depleted cells leads to severely altered vacuole morphology, perturbed vacuole assembly and strong inhibition of homotypic vacuole fusion...
  23. doi request reprint Lipid signalling in disease
    Matthias P Wymann
    Institute of Biochemistry and Genetics, Department of Biomedicine, University of Basel, Mattenstrasse 28, CH 4058 Basel, Switzerland
    Nat Rev Mol Cell Biol 9:162-76. 2008
    ....
  24. ncbi request reprint Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane
    Tomomi Kuwana
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Cell 111:331-42. 2002
    ..We conclude that mitochondrial protein release in apoptosis can be mediated by supramolecular openings in the outer mitochondrial membrane, promoted by BH3/Bax/lipid interaction and directly inhibited by Bcl-x(L)...
  25. pmc A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast
    Marlis Eisenkolb
    Institute of Biochemistry, Graz University of Technology, A 8010 Graz, Austria
    Mol Biol Cell 13:4414-28. 2002
    ....
  26. pmc Lipid-dependent subcellular relocalization of the acyl chain desaturase in yeast
    Verena Tatzer
    Department of Biochemistry, Graz University of Technology, A 8010 Graz, Austria
    Mol Biol Cell 13:4429-42. 2002
    ..A model is discussed in which the accumulation of saturated lipids in a microdomain around the desaturase could induce the observed segregation and relocalization of the enzyme...
  27. pmc Synthetic lethal interaction of the mitochondrial phosphatidylethanolamine biosynthetic machinery with the prohibitin complex of Saccharomyces cerevisiae
    Ruth Birner
    Institut fur Biochemie, Technische Universitat Graz, Austria
    Mol Biol Cell 14:370-83. 2003
    ..In conclusion, destabilizing effects triggered by low levels of mitochondrial PtdEtn seem to account for synthetic lethality of psd1Delta with phb mutants...
  28. pmc Differential regulation of ceramide synthase components LAC1 and LAG1 in Saccharomyces cerevisiae
    Marcin Kolaczkowski
    Department of Physiology and Biophysics, 6 530 Bowen Science Building, University of Iowa, Iowa City, IA 52242, USA
    Eukaryot Cell 3:880-92. 2004
    ..These data demonstrate extensive coordinate control of sphingolipid biosynthesis and multidrug resistance in yeast...
  29. pmc A genomewide screen reveals a role of mitochondria in anaerobic uptake of sterols in yeast
    Sonja Reiner
    Institute of Biochemistry, Graz University of Technology, 8010 Graz, Austria
    Mol Biol Cell 17:90-103. 2006
    ....
  30. pmc The Sur7p family defines novel cortical domains in Saccharomyces cerevisiae, affects sphingolipid metabolism, and is involved in sporulation
    Michael E Young
    Department of Cell Biology and Physiology, Washington University, St Louis, Missouri 63110, USA
    Mol Cell Biol 22:927-34. 2002
    ..In particular, the sphingoid base length and number of hydroxyl groups in inositol phosphorylceramides were altered in sur7Delta, ydl222Delta, and yne194Delta strains...