Thomas Ruckstuhl

Summary

Affiliation: University of Zurich
Country: Switzerland

Publications

  1. ncbi request reprint Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057, Zurich, Switzerland
    Biosens Bioelectron 18:1193-9. 2003
  2. ncbi request reprint Confocal reader for biochip screening and fluorescence microscopy
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    Biosens Bioelectron 20:1872-7. 2005
  3. ncbi request reprint Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Opt Lett 29:569-71. 2004
  4. pmc Acetabular fracture types vary with different acetabular version
    Clement M L Werner
    Division of Trauma Surgery, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland
    Int Orthop 36:2559-63. 2012
  5. doi request reprint Simultaneous near-field and far-field fluorescence microscopy of single molecules
    Thomas Ruckstuhl
    Institute of Physical Chemistry, University of Zurich, Zurich, Switzerland
    Opt Express 19:6836-44. 2011
  6. ncbi request reprint Microscope objective for large-angle fluorescence used for rapid detection of single nucleotide polymorphisms in DNA hybridization
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Anal Chem 77:2656-61. 2005
  7. doi request reprint Normal values of Wiberg's lateral center-edge angle and Lequesne's acetabular index--a coxometric update
    Clement M L Werner
    Division of Trauma Surgery, University Hospital Zurich, Raemistrasse 100, 8091 Zurich, Switzerland
    Skeletal Radiol 41:1273-8. 2012
  8. ncbi request reprint Fast detection of single nucleotide polymorphisms (SNPs) by primer elongation with monitoring of supercritical-angle fluorescence
    Alexander Krieg
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Chembiochem 5:1680-5. 2004
  9. ncbi request reprint Parallel two-channel near- and far-field fluorescence microscopy
    Dorinel Verdes
    Universitat Zurich, Physikalisch Chemisches Institut, Winterthurerstrasse 190, Zurich CH 8057, Switzerland
    J Biomed Opt 12:034012. 2007
  10. ncbi request reprint Conformational reorientation of immunoglobulin G during nonspecific interaction with surfaces
    Michael Rankl
    Institute of Physical Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Chemphyschem 7:837-46. 2006

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057, Zurich, Switzerland
    Biosens Bioelectron 18:1193-9. 2003
    ..The performance of the system is demonstrated by real time measurements of antibody-antigen reactions. Rate constants of the reaction were extracted. Antigen concentrations were detected down to 10(-13) mol/l...
  2. ncbi request reprint Confocal reader for biochip screening and fluorescence microscopy
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    Biosens Bioelectron 20:1872-7. 2005
    ..The versatility of the instrument is demonstrated by imaging cells and reading out a DNA-chip. The excellent sensitivity is shown by detecting single fluorescently labeled antibodies...
  3. ncbi request reprint Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Opt Lett 29:569-71. 2004
    ..The system delivers TIRF images with excellent spatial resolution and detects single molecules with a high signal-to-background ratio...
  4. pmc Acetabular fracture types vary with different acetabular version
    Clement M L Werner
    Division of Trauma Surgery, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland
    Int Orthop 36:2559-63. 2012
    ..Our study was carried out to clarify this issue...
  5. doi request reprint Simultaneous near-field and far-field fluorescence microscopy of single molecules
    Thomas Ruckstuhl
    Institute of Physical Chemistry, University of Zurich, Zurich, Switzerland
    Opt Express 19:6836-44. 2011
    ..The oil immersion objective, developed for use on common microscope bodies, opens up new possibilities for the study of topographies and dynamics at surfaces and on membranes...
  6. ncbi request reprint Microscope objective for large-angle fluorescence used for rapid detection of single nucleotide polymorphisms in DNA hybridization
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Anal Chem 77:2656-61. 2005
    ..This limitation is now overcome due to the new type of microscope objective, which produces an extremely small detection volume at a water/glass interface...
  7. doi request reprint Normal values of Wiberg's lateral center-edge angle and Lequesne's acetabular index--a coxometric update
    Clement M L Werner
    Division of Trauma Surgery, University Hospital Zurich, Raemistrasse 100, 8091 Zurich, Switzerland
    Skeletal Radiol 41:1273-8. 2012
    ....
  8. ncbi request reprint Fast detection of single nucleotide polymorphisms (SNPs) by primer elongation with monitoring of supercritical-angle fluorescence
    Alexander Krieg
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Chembiochem 5:1680-5. 2004
    ..We demonstrate the efficiency of the method by detecting an SNP in the ErbB2 gene that is involved in causing a higher risk of breast cancer...
  9. ncbi request reprint Parallel two-channel near- and far-field fluorescence microscopy
    Dorinel Verdes
    Universitat Zurich, Physikalisch Chemisches Institut, Winterthurerstrasse 190, Zurich CH 8057, Switzerland
    J Biomed Opt 12:034012. 2007
    ..Consequently, the signals from surface-bound and unbound diffusing fluorescent molecules can be obtained simultaneously...
  10. ncbi request reprint Conformational reorientation of immunoglobulin G during nonspecific interaction with surfaces
    Michael Rankl
    Institute of Physical Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Chemphyschem 7:837-46. 2006
    ..Unlike the rates of adsorption and desorption, the transition rate is independent of the type of surface and only influenced by the structural reorganization of the protein...
  11. ncbi request reprint Real-time detection of nucleotide incorporation during complementary DNA strand synthesis
    Alexander Krieg
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Chembiochem 4:589-92. 2003
    ..The efficiency of this reaction is of the same order of magnitude as that of a bimolecular hybridization reaction...
  12. doi request reprint Relationship between Wiberg's lateral center edge angle, Lequesne's acetabular index, and medial acetabular bone stock
    Clement M L Werner
    Department of Orthopaedics, Balgrist University Hospital Zurich, Zurich, Switzerland
    Skeletal Radiol 40:1435-9. 2011
    ..The aim of our study was to clarify the relationship between LCE, AI, and thickness of the medial acetabular wall...
  13. ncbi request reprint Nanometer axial resolution by three-dimensional supercritical angle fluorescence microscopy
    Christian M Winterflood
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Phys Rev Lett 105:108103. 2010
    ....
  14. pmc Sizing of single fluorescently stained DNA fragments by scanning microscopy
    Stephan Laib
    Institute for Physical Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Nucleic Acids Res 31:e138. 2003
    ..At this a separation of buffers or enzymes was unnecessary. DNA sizes were determined within an uncertainty of 7-14%. The proposed method is straightforward and can be applied to standardized microtiter plates...
  15. doi request reprint Supercritical angle fluorescence immunoassay platform
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH 8057 Zürich, Switzerland
    Anal Chem 83:2345-50. 2011
    ..5 pg/mL) and the linear range covered 3 orders of magnitude. The developed technology is also adaptable to well plates and has great potential of replacing the work-intensive and time-consuming enzyme-linked immunosorbant assay (ELISA)...
  16. doi request reprint Correlation of psychomotor findings and the outcome of a physical therapy program to treat scapular dyskinesis
    Clement M L Werner
    Department of Orthopaedics, University of Zurich, Uniklinik Balgrist, Zurich, Switzerland
    J Shoulder Elbow Surg 20:69-72. 2011
    ..This pilot study was performed to investigate the influence of psychomotor skills on the outcome of a specific nonoperative program (scapular dyskinesis-program) to treat scapular dyskinesis...
  17. doi request reprint Correlation of the cross-over ratio of the cross-over sign on conventional pelvic radiographs with computed tomography retroversion measurements
    Clement M L Werner
    Department of Orthopaedics, Uniklinik Balgrist, University of Zurich, Zurich, Switzerland
    Skeletal Radiol 39:655-60. 2010
    ..This would facilitate the interpretation of the cross-over sign regarding the amount of acetabular retroversion...
  18. ncbi request reprint Real-time detection of polymerase activity using supercritical angle fluorescence
    Alexander Krieg
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, 8057 Zurich, Switzerland
    J Fluoresc 14:75-8. 2004
    ..By comparison of the observed signal intensities it was possible to conclude that the system Cy5-dCTP-Klenow (exonuclease free) fragment gives the best incorporation yield of the investigated enzymes and dNTPs...
  19. pmc Supercritical angle fluorescence correlation spectroscopy
    Jonas Ries
    Biotechnologisches Zentrum, Technical University of Dresden, Dresden, Germany
    Biophys J 94:221-9. 2008
    ..We present an extensive theoretical framework that allows for accurate and quantitative evaluation of the SA-FCS correlation curves...