Research Topics
| Thomas RuckstuhlSummaryAffiliation: University of Zurich Country: Switzerland Publications
| Collaborators
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Detail Information
Publications
Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrumentThomas Ruckstuhl
Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057, Zurich, Switzerland
Biosens Bioelectron 18:1193-9. 2003..The performance of the system is demonstrated by real time measurements of antibody-antigen reactions. Rate constants of the reaction were extracted. Antigen concentrations were detected down to 10(-13) mol/l...- Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopyThomas Ruckstuhl
Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Opt Lett 29:569-71. 2004..The system delivers TIRF images with excellent spatial resolution and detects single molecules with a high signal-to-background ratio... - Confocal reader for biochip screening and fluorescence microscopyThomas Ruckstuhl
Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
Biosens Bioelectron 20:1872-7. 2005..The versatility of the instrument is demonstrated by imaging cells and reading out a DNA-chip. The excellent sensitivity is shown by detecting single fluorescently labeled antibodies... 
Simultaneous near-field and far-field fluorescence microscopy of single moleculesThomas Ruckstuhl
Institute of Physical Chemistry, University of Zurich, Zurich, Switzerland
Opt Express 19:6836-44. 2011..The oil immersion objective, developed for use on common microscope bodies, opens up new possibilities for the study of topographies and dynamics at surfaces and on membranes...- Microscope objective for large-angle fluorescence used for rapid detection of single nucleotide polymorphisms in DNA hybridizationThomas Ruckstuhl
Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Anal Chem 77:2656-61. 2005..This limitation is now overcome due to the new type of microscope objective, which produces an extremely small detection volume at a water/glass interface... 
Acetabular fracture types vary with different acetabular versionClement M L Werner
Division of Trauma Surgery, University Hospital Zurich, Raemistrasse 100, 8091, Zurich, Switzerland
Int Orthop 36:2559-63. 2012..Our study was carried out to clarify this issue...- Normal values of Wiberg's lateral center-edge angle and Lequesne's acetabular index--a coxometric updateClement M L Werner
Division of Trauma Surgery, University Hospital Zurich, Raemistrasse 100, 8091 Zurich, Switzerland
Skeletal Radiol 41:1273-8. 2012.... - Fast detection of single nucleotide polymorphisms (SNPs) by primer elongation with monitoring of supercritical-angle fluorescenceAlexander Krieg
Physikalisch Chemisches Institut, , Winterthurerstrasse 190, , Switzerland
Chembiochem 5:1680-5. 2004..We demonstrate the efficiency of the method by detecting an SNP in the ErbB2 gene that is involved in causing a higher risk of breast cancer... - Parallel two-channel near- and far-field fluorescence microscopyDorinel Verdes
Universitat Zurich, Physikalisch Chemisches Institut, Winterthurerstrasse 190, Zurich CH 8057, Switzerland
J Biomed Opt 12:034012. 2007..Consequently, the signals from surface-bound and unbound diffusing fluorescent molecules can be obtained simultaneously... - Conformational reorientation of immunoglobulin G during nonspecific interaction with surfacesMichael Rankl
Institute of Physical Chemistry, University of Zurich, Winterthurerstrasse 190, , Switzerland
Chemphyschem 7:837-46. 2006..Unlike the rates of adsorption and desorption, the transition rate is independent of the type of surface and only influenced by the structural reorganization of the protein... - Real-time detection of nucleotide incorporation during complementary DNA strand synthesisAlexander Krieg
Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
Chembiochem 4:589-92. 2003..The efficiency of this reaction is of the same order of magnitude as that of a bimolecular hybridization reaction... - Relationship between Wiberg's lateral center edge angle, Lequesne's acetabular index, and medial acetabular bone stockClement M L Werner
Department of Orthopaedics, Balgrist University Hospital Zurich, Zurich, Switzerland
Skeletal Radiol 40:1435-9. 2011..The aim of our study was to clarify the relationship between LCE, AI, and thickness of the medial acetabular wall... - Nanometer axial resolution by three-dimensional supercritical angle fluorescence microscopyChristian M Winterflood
Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Phys Rev Lett 105:108103. 2010.... - Sizing of single fluorescently stained DNA fragments by scanning microscopyStephan Laib
Institute for Physical Chemistry, , Winterthurerstrasse 190, , Switzerland
Nucleic Acids Res 31:e138. 2003..At this a separation of buffers or enzymes was unnecessary. DNA sizes were determined within an uncertainty of 7-14%. The proposed method is straightforward and can be applied to standardized microtiter plates... - Supercritical angle fluorescence immunoassay platformThomas Ruckstuhl
Physikalisch Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH 8057 Zürich, Switzerland
Anal Chem 83:2345-50. 2011..5 pg/mL) and the linear range covered 3 orders of magnitude. The developed technology is also adaptable to well plates and has great potential of replacing the work-intensive and time-consuming enzyme-linked immunosorbant assay (ELISA)... - Correlation of psychomotor findings and the outcome of a physical therapy program to treat scapular dyskinesisClement M L Werner
Department of Orthopaedics, University of Zurich, Uniklinik Balgrist, Zurich, Switzerland
J Shoulder Elbow Surg 20:69-72. 2011..This pilot study was performed to investigate the influence of psychomotor skills on the outcome of a specific nonoperative program (scapular dyskinesis-program) to treat scapular dyskinesis... - Correlation of the cross-over ratio of the cross-over sign on conventional pelvic radiographs with computed tomography retroversion measurementsClement M L Werner
Department of Orthopaedics, Uniklinik Balgrist, University of Zurich, Zurich, Switzerland
Skeletal Radiol 39:655-60. 2010..This would facilitate the interpretation of the cross-over sign regarding the amount of acetabular retroversion... - Real-time detection of polymerase activity using supercritical angle fluorescenceAlexander Krieg
Physikalisch-Chemisches Institut, , Winterthurerstr. 190, , Switzerland
J Fluoresc 14:75-8. 2004..By comparison of the observed signal intensities it was possible to conclude that the system Cy5-dCTP-Klenow (exonuclease free) fragment gives the best incorporation yield of the investigated enzymes and dNTPs... - Supercritical angle fluorescence correlation spectroscopyJonas Ries
Biotechnologisches Zentrum, Technical University of Dresden, Dresden, Germany
Biophys J 94:221-9. 2008..We present an extensive theoretical framework that allows for accurate and quantitative evaluation of the SA-FCS correlation curves...