Research Topics
Species | Barbara Rothen-RutishauserSummaryAffiliation: University of Bern Country: Switzerland Publications
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Detail Information
Publications
Quantitative evaluation of cellular uptake and trafficking of plain and polyethylene glycol-coated gold nanoparticlesChristina Brandenberger
Institute of Anatomy, University of Bern, Bern 3012, Switzerland
Small 6:1669-78. 2010..Other nonendocytotic entry mechanisms are found to be involved as well, as indicated by localization of a minority of PEG-coated NPs in the cytosol...
The adsorption of biomolecules to multi-walled carbon nanotubes is influenced by both pulmonary surfactant lipids and surface chemistryMichael Gasser
Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Materials Biology Interactions, St, Gallen, Switzerland
J Nanobiotechnology 8:31. 2010..abstract:..
Direct combination of nanoparticle fabrication and exposure to lung cell cultures in a closed setup as a method to simulate accidental nanoparticle exposure of humansBarbara Rothen-Rutishauser
Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
Environ Sci Technol 43:2634-40. 2009..The direct combination of production and exposure allows studying particle toxicity in a simple and reproducible way under environmental conditions...- Interaction of fine particles and nanoparticles with red blood cells visualized with advanced microscopic techniquesBarbara M Rothen-Rutishauser
Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
Environ Sci Technol 40:4353-9. 2006..These results suggest that particles may penetrate the red blood cell membrane by a still unknown mechanism different from phagocytosis and endocytosis... - Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airwaysLoretta Müller
Institute of Anatomy, Division of Histology, University of Bern, Balzerstrasse 2, 3000 Bern 9, Switzerland
J R Soc Interface 7:S27-40. 2010..The interplay of different lung cell types seems to substantially modulate the oxidative stress and the inflammatory responses after NP exposure... - Cerium oxide nanoparticle uptake kinetics from the gas-phase into lung cells in vitro is transport limitedDavid O Raemy
Division of Histology, Institute of Anatomy, University of Bern, Bern, Switzerland
Eur J Pharm Biopharm 77:368-75. 2011..The uptake behaviour of aerosolized CeO(2) is in line with observations on cerium suspensions, where particle mass transport was identified as the rate-limiting factor for NP internalization... - Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cellsMarianne Geiser
Institute for Anatomy, University of Bern, Bern, Switzerland
Environ Health Perspect 113:1555-60. 2005..Particles within cells are not membrane bound and hence have direct access to intracellular proteins, organelles, and DNA, which may greatly enhance their toxic potential... - Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrierBarbara Rothen-Rutishauser
Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
Swiss Med Wkly 140:w13060. 2010..We have used this tool to establish and evaluate a three dimensional model of the human epithelial airway wall... - Connexin43 ablation in foetal atrial myocytes decreases electrical coupling, partner connexins, and sodium currentThomas Desplantez
Department of Physiology, University of Bern, Bern, Switzerland
Cardiovasc Res 94:58-65. 2012.... - The uptake and intracellular fate of a series of different surface coated quantum dots in vitroMartin J D Clift
Department of Clinical Research, Division of Pneumology, Inselspital University Hospital, University of Bern, Postfach 44, Bern 3008, Switzerland
Toxicology 286:58-68. 2011..The data from this study combined with current knowledge, indicates that the intracellular localisation of QDs could be directly related to their toxicity... - Fluorescent-magnetic hybrid nanoparticles induce a dose-dependent increase in proinflammatory response in lung cells in vitro correlated with intracellular localizationAndrea D Lehmann
Institute of Anatomy, University of Bern, Bern 3012, Switzerland
Small 6:753-62. 2010..It is concluded that the uptake behavior and the proinflammatory response upon particle exposure are dependent on the time, cell type, and cell culture... - New exposure system to evaluate the toxicity of (scooter) exhaust emissions in lung cells in vitroLoretta Müller
Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, Bern 9, Switzerland
Environ Sci Technol 44:2632-8. 2010.... - A newly developed in vitro model of the human epithelial airway barrier to study the toxic potential of nanoparticlesBarbara Rothen-Rutishauser
Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
ALTEX 25:191-6. 2008..Our in vitro model of the epithelial airway barrier offers a valuable tool to study the interaction of particles with lung cells at a nanostructural level and to investigate the toxic potential of nanoparticles... - Intracellular imaging of nanoparticles: is it an elemental mistake to believe what you see?Christina Brandenberger
Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000, Bern 9, Switzerland
Part Fibre Toxicol 7:15. 2010..This observation emphasizes the necessity to perform elemental analysis when investigating intracellular NP localization using TEM... - Interactions of nanoparticles with pulmonary structures and cellular responsesChristian Mühlfeld
Institute of Anatomy, Univ of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
Am J Physiol Lung Cell Mol Physiol 294:L817-29. 2008..Furthermore, this review places special emphasis on methodological differences between experimental studies and the caveats associated with the dose metrics and points out ways to overcome inherent methodological problems... - In vitro models of the human epithelial airway barrier to study the toxic potential of particulate matterBarbara Rothen-Rutishauser
University of Bern, Institute of Anatomy, Division of Histology, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
Expert Opin Drug Metab Toxicol 4:1075-89. 2008..Of particular interest are the ultrafine particles that are particularly toxic. In addition more and more nanoparticles are released into the environment; however, the potential health effects of these nanoparticles are yet unknown... - Nanotoxicology: a perspective and discussion of whether or not in vitro testing is a valid alternativeMartin J D Clift
Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
Arch Toxicol 85:723-31. 2011.... - Dendritic cells and macrophages form a transepithelial network against foreign particulate antigensFabian Blank
Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
Am J Respir Cell Mol Biol 36:669-77. 2007.... - Visualization and quantitative analysis of nanoparticles in the respiratory tract by transmission electron microscopyChristian Mühlfeld
Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
Part Fibre Toxicol 4:11. 2007..This comprehensive article aims to provide a basis for scientists in nanoparticle research to integrate electron microscopic analyses into their study design and to select the appropriate microscopic strategy... - Translocation and cellular entering mechanisms of nanoparticles in the respiratory tractChristian Mühlfeld
Institute of Anatomy, University of Bern, Bern, Switzerland
Swiss Med Wkly 138:387-91. 2008.... - Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cellsFabian Blank
Department of Clinical Research, Division of Pulmonology, Bern University Hospital, Bern, Switzerland
Nanotoxicology 5:606-21. 2011..The decreased antigen processing and CD4+ T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure... - Toxic effects of brake wear particles on epithelial lung cells in vitroMichael Gasser
Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
Part Fibre Toxicol 6:30. 2009..abstract:.. - Virosomes can enter cells by non-phagocytic mechanismsUrsula Hofer
Institute of Anatomy, Division of Histology, University of Bern, Switzerland
J Liposome Res 19:301-9. 2009..Further, they can penetrate the airway barrier and reach resident dendritic cells. Therefore, virosomes are promising vaccine candidates... - Re-evaluation of pulmonary titanium dioxide nanoparticle distribution using the "relative deposition index": Evidence for clearance through microvasculatureChristian Mühlfeld
Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
Part Fibre Toxicol 4:7. 2007..abstract:.. - Particles induce apical plasma membrane enlargement in epithelial lung cell line depending on particle surface area doseChristina Brandenberger
Institute of Anatomy, University of Bern, Bern, Switzerland
Respir Res 10:22. 2009..1 microm) particles may be associated with different effects on the APM. Therefore, we studied particle-induced changes in APM surface area in relation to applied and intracellular particle size, surface and number... - Fate of inhaled particles after interaction with the lung surfacePeter Gehr
Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000, Bern 9, Switzerland
Paediatr Respir Rev 7:S73-5. 2006..Furthermore, the cellular response was determined by measurement of TNF-alpha. Dendritic cells gained access to the apical side of the epithelium where they sampled particles and interacted with macrophages... - An optimized in vitro model of the respiratory tract wall to study particle cell interactionsFabian Blank
Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
J Aerosol Med 19:392-405. 2006..In this study, we present an in vitro model of the respiratory tract wall consisting of air-exposed lung epithelial cells covered by a liquid lining layer with a surfactant film to study particle-cell interactions... - A three-dimensional cellular model of the human respiratory tract to study the interaction with particlesBarbara M Rothen-Rutishauser
Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
Am J Respir Cell Mol Biol 32:281-9. 2005..Particles were found in all three cell types, although dendritic cells were not directly exposed to the particles. More investigations are needed to understand the translocation pathway... - Differential effects of long and short carbon nanotubes on the gas-exchange region of the mouse lungChristian Mühlfeld
Institute of Anatomy, University of Bern, Bern, Switzerland
Nanotoxicology 6:867-79. 2012..We observed mild inflammatory and pathological responses to short and long MWCNT in the lung parenchyma depending on the size of the applied MWCNT... - Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway modelBarbara Rothen-Rutishauser
Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
Part Fibre Toxicol 4:9. 2007..abstract:.. - A novel quantitative method for analyzing the distributions of nanoparticles between different tissue and intracellular compartmentsChristian Mühlfeld
University of Bern, Institute of Anatomy, Division of Histology, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
J Aerosol Med 20:395-407. 2007..We first describe the preconditions and the way to implement these methods, then provide three worked examples, and finally discuss the advantages, pitfalls, and limitations of this method... - Effects of combustion-derived ultrafine particles and manufactured nanoparticles on heart cells in vitroMaria Helfenstein
Institute for Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
Toxicology 253:70-8. 2008..These results suggest that diesel exhaust as well as titanium dioxide particles and to a lesser extent also single-walled carbon nanotubes can directly induce cardiac cell damage and can affect the function of the cells... - Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductanceMegan L McCain
Department of Physiology, University of Bern, Bern, Switzerland
Am J Physiol Heart Circ Physiol 302:H443-50. 2012..Our results show a linear correlation between g(j) and Cx43 immunosignal within a range of 8-50 nS...