Barbara Rothen-Rutishauser

Summary

Affiliation: University of Bern
Country: Switzerland

Publications

  1. ncbi Quantitative evaluation of cellular uptake and trafficking of plain and polyethylene glycol-coated gold nanoparticles
    Christina Brandenberger
    Institute of Anatomy, University of Bern, Bern 3012, Switzerland
    Small 6:1669-78. 2010
  2. pmc The adsorption of biomolecules to multi-walled carbon nanotubes is influenced by both pulmonary surfactant lipids and surface chemistry
    Michael Gasser
    Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Materials Biology Interactions, St, Gallen, Switzerland
    J Nanobiotechnology 8:31. 2010
  3. ncbi Direct combination of nanoparticle fabrication and exposure to lung cell cultures in a closed setup as a method to simulate accidental nanoparticle exposure of humans
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Environ Sci Technol 43:2634-40. 2009
  4. ncbi Interaction of fine particles and nanoparticles with red blood cells visualized with advanced microscopic techniques
    Barbara M Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Environ Sci Technol 40:4353-9. 2006
  5. pmc Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airways
    Loretta Müller
    Institute of Anatomy, Division of Histology, University of Bern, Balzerstrasse 2, 3000 Bern 9, Switzerland
    J R Soc Interface 7:S27-40. 2010
  6. doi Cerium oxide nanoparticle uptake kinetics from the gas-phase into lung cells in vitro is transport limited
    David O Raemy
    Division of Histology, Institute of Anatomy, University of Bern, Bern, Switzerland
    Eur J Pharm Biopharm 77:368-75. 2011
  7. pmc Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells
    Marianne Geiser
    Institute for Anatomy, University of Bern, Bern, Switzerland
    Environ Health Perspect 113:1555-60. 2005
  8. doi Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Swiss Med Wkly 140:w13060. 2010
  9. pmc Connexin43 ablation in foetal atrial myocytes decreases electrical coupling, partner connexins, and sodium current
    Thomas Desplantez
    Department of Physiology, University of Bern, Bern, Switzerland
    Cardiovasc Res 94:58-65. 2012
  10. doi The uptake and intracellular fate of a series of different surface coated quantum dots in vitro
    Martin J D Clift
    Department of Clinical Research, Division of Pneumology, Inselspital University Hospital, University of Bern, Postfach 44, Bern 3008, Switzerland
    Toxicology 286:58-68. 2011

Collaborators

Detail Information

Publications35

  1. ncbi Quantitative evaluation of cellular uptake and trafficking of plain and polyethylene glycol-coated gold nanoparticles
    Christina Brandenberger
    Institute of Anatomy, University of Bern, Bern 3012, Switzerland
    Small 6:1669-78. 2010
    ..Other nonendocytotic entry mechanisms are found to be involved as well, as indicated by localization of a minority of PEG-coated NPs in the cytosol...
  2. pmc The adsorption of biomolecules to multi-walled carbon nanotubes is influenced by both pulmonary surfactant lipids and surface chemistry
    Michael Gasser
    Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Materials Biology Interactions, St, Gallen, Switzerland
    J Nanobiotechnology 8:31. 2010
    ..abstract:..
  3. ncbi Direct combination of nanoparticle fabrication and exposure to lung cell cultures in a closed setup as a method to simulate accidental nanoparticle exposure of humans
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Environ Sci Technol 43:2634-40. 2009
    ..The direct combination of production and exposure allows studying particle toxicity in a simple and reproducible way under environmental conditions...
  4. ncbi Interaction of fine particles and nanoparticles with red blood cells visualized with advanced microscopic techniques
    Barbara M Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Environ Sci Technol 40:4353-9. 2006
    ..These results suggest that particles may penetrate the red blood cell membrane by a still unknown mechanism different from phagocytosis and endocytosis...
  5. pmc Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airways
    Loretta Müller
    Institute of Anatomy, Division of Histology, University of Bern, Balzerstrasse 2, 3000 Bern 9, Switzerland
    J R Soc Interface 7:S27-40. 2010
    ..The interplay of different lung cell types seems to substantially modulate the oxidative stress and the inflammatory responses after NP exposure...
  6. doi Cerium oxide nanoparticle uptake kinetics from the gas-phase into lung cells in vitro is transport limited
    David O Raemy
    Division of Histology, Institute of Anatomy, University of Bern, Bern, Switzerland
    Eur J Pharm Biopharm 77:368-75. 2011
    ..The uptake behaviour of aerosolized CeO(2) is in line with observations on cerium suspensions, where particle mass transport was identified as the rate-limiting factor for NP internalization...
  7. pmc Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells
    Marianne Geiser
    Institute for Anatomy, University of Bern, Bern, Switzerland
    Environ Health Perspect 113:1555-60. 2005
    ..Particles within cells are not membrane bound and hence have direct access to intracellular proteins, organelles, and DNA, which may greatly enhance their toxic potential...
  8. doi Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Swiss Med Wkly 140:w13060. 2010
    ..We have used this tool to establish and evaluate a three dimensional model of the human epithelial airway wall...
  9. pmc Connexin43 ablation in foetal atrial myocytes decreases electrical coupling, partner connexins, and sodium current
    Thomas Desplantez
    Department of Physiology, University of Bern, Bern, Switzerland
    Cardiovasc Res 94:58-65. 2012
    ....
  10. doi The uptake and intracellular fate of a series of different surface coated quantum dots in vitro
    Martin J D Clift
    Department of Clinical Research, Division of Pneumology, Inselspital University Hospital, University of Bern, Postfach 44, Bern 3008, Switzerland
    Toxicology 286:58-68. 2011
    ..The data from this study combined with current knowledge, indicates that the intracellular localisation of QDs could be directly related to their toxicity...
  11. doi Endocytosis of environmental and engineered micro- and nanosized particles
    Peter Gehr
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Compr Physiol 1:1159-74. 2011
    ..However, with NPs it is different. NPs may not be eliminated easily, and, hence may stimulate the cells to react in an unfavorable way. What we can learn is that NPs behave differently than microparticles...
  12. doi Size-dependent uptake of particles by pulmonary antigen-presenting cell populations and trafficking to regional lymph nodes
    Fabian Blank
    Department of Respiratory Medicine, Bern University Hospital, Bern, Switzerland
    Am J Respir Cell Mol Biol 49:67-77. 2013
    ....
  13. doi Differential effects of long and short carbon nanotubes on the gas-exchange region of the mouse lung
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Nanotoxicology 6:867-79. 2012
    ..We observed mild inflammatory and pathological responses to short and long MWCNT in the lung parenchyma depending on the size of the applied MWCNT...
  14. doi New exposure system to evaluate the toxicity of (scooter) exhaust emissions in lung cells in vitro
    Loretta Müller
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, Bern 9, Switzerland
    Environ Sci Technol 44:2632-8. 2010
    ....
  15. doi Fluorescent-magnetic hybrid nanoparticles induce a dose-dependent increase in proinflammatory response in lung cells in vitro correlated with intracellular localization
    Andrea D Lehmann
    Institute of Anatomy, University of Bern, Bern 3012, Switzerland
    Small 6:753-62. 2010
    ..It is concluded that the uptake behavior and the proinflammatory response upon particle exposure are dependent on the time, cell type, and cell culture...
  16. pmc Intracellular imaging of nanoparticles: is it an elemental mistake to believe what you see?
    Christina Brandenberger
    Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000, Bern 9, Switzerland
    Part Fibre Toxicol 7:15. 2010
    ..This observation emphasizes the necessity to perform elemental analysis when investigating intracellular NP localization using TEM...
  17. doi Interactions of nanoparticles with pulmonary structures and cellular responses
    Christian Mühlfeld
    Institute of Anatomy, Univ of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Am J Physiol Lung Cell Mol Physiol 294:L817-29. 2008
    ..Furthermore, this review places special emphasis on methodological differences between experimental studies and the caveats associated with the dose metrics and points out ways to overcome inherent methodological problems...
  18. ncbi A newly developed in vitro model of the human epithelial airway barrier to study the toxic potential of nanoparticles
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    ALTEX 25:191-6. 2008
    ..Our in vitro model of the epithelial airway barrier offers a valuable tool to study the interaction of particles with lung cells at a nanostructural level and to investigate the toxic potential of nanoparticles...
  19. ncbi In vitro models of the human epithelial airway barrier to study the toxic potential of particulate matter
    Barbara Rothen-Rutishauser
    University of Bern, Institute of Anatomy, Division of Histology, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Expert Opin Drug Metab Toxicol 4:1075-89. 2008
    ..Of particular interest are the ultrafine particles that are particularly toxic. In addition more and more nanoparticles are released into the environment; however, the potential health effects of these nanoparticles are yet unknown...
  20. doi Nanotoxicology: a perspective and discussion of whether or not in vitro testing is a valid alternative
    Martin J D Clift
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Arch Toxicol 85:723-31. 2011
    ....
  21. ncbi Dendritic cells and macrophages form a transepithelial network against foreign particulate antigens
    Fabian Blank
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Am J Respir Cell Mol Biol 36:669-77. 2007
    ....
  22. doi Translocation and cellular entering mechanisms of nanoparticles in the respiratory tract
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Swiss Med Wkly 138:387-91. 2008
    ....
  23. pmc Visualization and quantitative analysis of nanoparticles in the respiratory tract by transmission electron microscopy
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Part Fibre Toxicol 4:11. 2007
    ..This comprehensive article aims to provide a basis for scientists in nanoparticle research to integrate electron microscopic analyses into their study design and to select the appropriate microscopic strategy...
  24. doi Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells
    Fabian Blank
    Department of Clinical Research, Division of Pulmonology, Bern University Hospital, Bern, Switzerland
    Nanotoxicology 5:606-21. 2011
    ..The decreased antigen processing and CD4+ T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure...
  25. pmc Toxic effects of brake wear particles on epithelial lung cells in vitro
    Michael Gasser
    Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 6:30. 2009
    ..abstract:..
  26. doi Virosomes can enter cells by non-phagocytic mechanisms
    Ursula Hofer
    Institute of Anatomy, Division of Histology, University of Bern, Switzerland
    J Liposome Res 19:301-9. 2009
    ..Further, they can penetrate the airway barrier and reach resident dendritic cells. Therefore, virosomes are promising vaccine candidates...
  27. pmc Re-evaluation of pulmonary titanium dioxide nanoparticle distribution using the "relative deposition index": Evidence for clearance through microvasculature
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Part Fibre Toxicol 4:7. 2007
    ..abstract:..
  28. pmc Particles induce apical plasma membrane enlargement in epithelial lung cell line depending on particle surface area dose
    Christina Brandenberger
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Respir Res 10:22. 2009
    ..1 microm) particles may be associated with different effects on the APM. Therefore, we studied particle-induced changes in APM surface area in relation to applied and intracellular particle size, surface and number...
  29. ncbi Fate of inhaled particles after interaction with the lung surface
    Peter Gehr
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000, Bern 9, Switzerland
    Paediatr Respir Rev 7:S73-5. 2006
    ..Furthermore, the cellular response was determined by measurement of TNF-alpha. Dendritic cells gained access to the apical side of the epithelium where they sampled particles and interacted with macrophages...
  30. ncbi An optimized in vitro model of the respiratory tract wall to study particle cell interactions
    Fabian Blank
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    J Aerosol Med 19:392-405. 2006
    ..In this study, we present an in vitro model of the respiratory tract wall consisting of air-exposed lung epithelial cells covered by a liquid lining layer with a surfactant film to study particle-cell interactions...
  31. ncbi A three-dimensional cellular model of the human respiratory tract to study the interaction with particles
    Barbara M Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Am J Respir Cell Mol Biol 32:281-9. 2005
    ..Particles were found in all three cell types, although dendritic cells were not directly exposed to the particles. More investigations are needed to understand the translocation pathway...
  32. ncbi A novel quantitative method for analyzing the distributions of nanoparticles between different tissue and intracellular compartments
    Christian Mühlfeld
    University of Bern, Institute of Anatomy, Division of Histology, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    J Aerosol Med 20:395-407. 2007
    ..We first describe the preconditions and the way to implement these methods, then provide three worked examples, and finally discuss the advantages, pitfalls, and limitations of this method...
  33. pmc Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway model
    Barbara Rothen-Rutishauser
    Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 4:9. 2007
    ..abstract:..
  34. doi Effects of combustion-derived ultrafine particles and manufactured nanoparticles on heart cells in vitro
    Maria Helfenstein
    Institute for Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Toxicology 253:70-8. 2008
    ..These results suggest that diesel exhaust as well as titanium dioxide particles and to a lesser extent also single-walled carbon nanotubes can directly induce cardiac cell damage and can affect the function of the cells...
  35. pmc Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance
    Megan L McCain
    Department of Physiology, University of Bern, Bern, Switzerland
    Am J Physiol Heart Circ Physiol 302:H443-50. 2012
    ..Our results show a linear correlation between g(j) and Cx43 immunosignal within a range of 8-50 nS...