Barbara Rothen-Rutishauser

Summary

Affiliation: University of Bern
Country: Switzerland

Publications

  1. doi Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Swiss Med Wkly 140:w13060. 2010
  2. ncbi In vitro models of the human epithelial airway barrier to study the toxic potential of particulate matter
    Barbara Rothen-Rutishauser
    University of Bern, Institute of Anatomy, Division of Histology, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Expert Opin Drug Metab Toxicol 4:1075-89. 2008
  3. pmc Toxic effects of brake wear particles on epithelial lung cells in vitro
    Michael Gasser
    Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 6:30. 2009
  4. ncbi A newly developed in vitro model of the human epithelial airway barrier to study the toxic potential of nanoparticles
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    ALTEX 25:191-6. 2008
  5. pmc A dose-controlled system for air-liquid interface cell exposure and application to zinc oxide nanoparticles
    Anke Gabriele Lenz
    Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Institute of Lung Biology and Disease, Ingolstaedter Landstrasse 1, D 85758 Neuherberg, Germany
    Part Fibre Toxicol 6:32. 2009
  6. pmc Diesel exhaust particles modulate the tight junction protein occludin in lung cells in vitro
    Andrea D Lehmann
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 6:26. 2009
  7. pmc Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway model
    Barbara Rothen-Rutishauser
    Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 4:9. 2007
  8. ncbi A three-dimensional cellular model of the human respiratory tract to study the interaction with particles
    Barbara M Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Am J Respir Cell Mol Biol 32:281-9. 2005
  9. pmc Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airways
    Loretta Müller
    Institute of Anatomy, Division of Histology, University of Bern, Balzerstrasse 2, 3000 Bern 9, Switzerland
    J R Soc Interface 7:S27-40. 2010
  10. doi Cerium oxide nanoparticle uptake kinetics from the gas-phase into lung cells in vitro is transport limited
    David O Raemy
    Division of Histology, Institute of Anatomy, University of Bern, Bern, Switzerland
    Eur J Pharm Biopharm 77:368-75. 2011

Collaborators

Detail Information

Publications39

  1. doi Laser scanning microscopy combined with image restoration to analyse a 3D model of the human epithelial airway barrier
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Swiss Med Wkly 140:w13060. 2010
    ..We have used this tool to establish and evaluate a three dimensional model of the human epithelial airway wall...
  2. ncbi In vitro models of the human epithelial airway barrier to study the toxic potential of particulate matter
    Barbara Rothen-Rutishauser
    University of Bern, Institute of Anatomy, Division of Histology, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Expert Opin Drug Metab Toxicol 4:1075-89. 2008
    ..Of particular interest are the ultrafine particles that are particularly toxic. In addition more and more nanoparticles are released into the environment; however, the potential health effects of these nanoparticles are yet unknown...
  3. pmc Toxic effects of brake wear particles on epithelial lung cells in vitro
    Michael Gasser
    Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 6:30. 2009
    ..abstract:..
  4. ncbi A newly developed in vitro model of the human epithelial airway barrier to study the toxic potential of nanoparticles
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    ALTEX 25:191-6. 2008
    ..Our in vitro model of the epithelial airway barrier offers a valuable tool to study the interaction of particles with lung cells at a nanostructural level and to investigate the toxic potential of nanoparticles...
  5. pmc A dose-controlled system for air-liquid interface cell exposure and application to zinc oxide nanoparticles
    Anke Gabriele Lenz
    Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Institute of Lung Biology and Disease, Ingolstaedter Landstrasse 1, D 85758 Neuherberg, Germany
    Part Fibre Toxicol 6:32. 2009
    ....
  6. pmc Diesel exhaust particles modulate the tight junction protein occludin in lung cells in vitro
    Andrea D Lehmann
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 6:26. 2009
    ..abstract:..
  7. pmc Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway model
    Barbara Rothen-Rutishauser
    Institute for Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Part Fibre Toxicol 4:9. 2007
    ..abstract:..
  8. ncbi A three-dimensional cellular model of the human respiratory tract to study the interaction with particles
    Barbara M Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Am J Respir Cell Mol Biol 32:281-9. 2005
    ..Particles were found in all three cell types, although dendritic cells were not directly exposed to the particles. More investigations are needed to understand the translocation pathway...
  9. pmc Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airways
    Loretta Müller
    Institute of Anatomy, Division of Histology, University of Bern, Balzerstrasse 2, 3000 Bern 9, Switzerland
    J R Soc Interface 7:S27-40. 2010
    ..The interplay of different lung cell types seems to substantially modulate the oxidative stress and the inflammatory responses after NP exposure...
  10. doi Cerium oxide nanoparticle uptake kinetics from the gas-phase into lung cells in vitro is transport limited
    David O Raemy
    Division of Histology, Institute of Anatomy, University of Bern, Bern, Switzerland
    Eur J Pharm Biopharm 77:368-75. 2011
    ..The uptake behaviour of aerosolized CeO(2) is in line with observations on cerium suspensions, where particle mass transport was identified as the rate-limiting factor for NP internalization...
  11. pmc Particles induce apical plasma membrane enlargement in epithelial lung cell line depending on particle surface area dose
    Christina Brandenberger
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Respir Res 10:22. 2009
    ..1 microm) particles may be associated with different effects on the APM. Therefore, we studied particle-induced changes in APM surface area in relation to applied and intracellular particle size, surface and number...
  12. pmc Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells
    Marianne Geiser
    Institute for Anatomy, University of Bern, Bern, Switzerland
    Environ Health Perspect 113:1555-60. 2005
    ..Particles within cells are not membrane bound and hence have direct access to intracellular proteins, organelles, and DNA, which may greatly enhance their toxic potential...
  13. pmc Connexin43 ablation in foetal atrial myocytes decreases electrical coupling, partner connexins, and sodium current
    Thomas Desplantez
    Department of Physiology, University of Bern, Bern, Switzerland
    Cardiovasc Res 94:58-65. 2012
    ....
  14. ncbi Dendritic cells and macrophages form a transepithelial network against foreign particulate antigens
    Fabian Blank
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Am J Respir Cell Mol Biol 36:669-77. 2007
    ....
  15. doi The uptake and intracellular fate of a series of different surface coated quantum dots in vitro
    Martin J D Clift
    Department of Clinical Research, Division of Pneumology, Inselspital University Hospital, University of Bern, Postfach 44, Bern 3008, Switzerland
    Toxicology 286:58-68. 2011
    ..The data from this study combined with current knowledge, indicates that the intracellular localisation of QDs could be directly related to their toxicity...
  16. doi Endocytosis of environmental and engineered micro- and nanosized particles
    Peter Gehr
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Compr Physiol 1:1159-74. 2011
    ..However, with NPs it is different. NPs may not be eliminated easily, and, hence may stimulate the cells to react in an unfavorable way. What we can learn is that NPs behave differently than microparticles...
  17. doi Size-dependent uptake of particles by pulmonary antigen-presenting cell populations and trafficking to regional lymph nodes
    Fabian Blank
    Department of Respiratory Medicine, Bern University Hospital, Bern, Switzerland
    Am J Respir Cell Mol Biol 49:67-77. 2013
    ....
  18. doi Differential effects of long and short carbon nanotubes on the gas-exchange region of the mouse lung
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Nanotoxicology 6:867-79. 2012
    ..We observed mild inflammatory and pathological responses to short and long MWCNT in the lung parenchyma depending on the size of the applied MWCNT...
  19. doi Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells
    Fabian Blank
    Department of Clinical Research, Division of Pulmonology, Bern University Hospital, Bern, Switzerland
    Nanotoxicology 5:606-21. 2011
    ..The decreased antigen processing and CD4+ T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure...
  20. pmc Intracellular imaging of nanoparticles: is it an elemental mistake to believe what you see?
    Christina Brandenberger
    Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000, Bern 9, Switzerland
    Part Fibre Toxicol 7:15. 2010
    ..This observation emphasizes the necessity to perform elemental analysis when investigating intracellular NP localization using TEM...
  21. ncbi Quantitative evaluation of cellular uptake and trafficking of plain and polyethylene glycol-coated gold nanoparticles
    Christina Brandenberger
    Institute of Anatomy, University of Bern, Bern 3012, Switzerland
    Small 6:1669-78. 2010
    ..Other nonendocytotic entry mechanisms are found to be involved as well, as indicated by localization of a minority of PEG-coated NPs in the cytosol...
  22. doi Interactions of nanoparticles with pulmonary structures and cellular responses
    Christian Mühlfeld
    Institute of Anatomy, Univ of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Am J Physiol Lung Cell Mol Physiol 294:L817-29. 2008
    ..Furthermore, this review places special emphasis on methodological differences between experimental studies and the caveats associated with the dose metrics and points out ways to overcome inherent methodological problems...
  23. doi New exposure system to evaluate the toxicity of (scooter) exhaust emissions in lung cells in vitro
    Loretta Müller
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, Bern 9, Switzerland
    Environ Sci Technol 44:2632-8. 2010
    ....
  24. doi Fluorescent-magnetic hybrid nanoparticles induce a dose-dependent increase in proinflammatory response in lung cells in vitro correlated with intracellular localization
    Andrea D Lehmann
    Institute of Anatomy, University of Bern, Bern 3012, Switzerland
    Small 6:753-62. 2010
    ..It is concluded that the uptake behavior and the proinflammatory response upon particle exposure are dependent on the time, cell type, and cell culture...
  25. doi Nanotoxicology: a perspective and discussion of whether or not in vitro testing is a valid alternative
    Martin J D Clift
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Arch Toxicol 85:723-31. 2011
    ....
  26. pmc Visualization and quantitative analysis of nanoparticles in the respiratory tract by transmission electron microscopy
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Part Fibre Toxicol 4:11. 2007
    ..This comprehensive article aims to provide a basis for scientists in nanoparticle research to integrate electron microscopic analyses into their study design and to select the appropriate microscopic strategy...
  27. doi Virosomes can enter cells by non-phagocytic mechanisms
    Ursula Hofer
    Institute of Anatomy, Division of Histology, University of Bern, Switzerland
    J Liposome Res 19:301-9. 2009
    ..Further, they can penetrate the airway barrier and reach resident dendritic cells. Therefore, virosomes are promising vaccine candidates...
  28. ncbi Direct combination of nanoparticle fabrication and exposure to lung cell cultures in a closed setup as a method to simulate accidental nanoparticle exposure of humans
    Barbara Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Environ Sci Technol 43:2634-40. 2009
    ..The direct combination of production and exposure allows studying particle toxicity in a simple and reproducible way under environmental conditions...
  29. doi Translocation and cellular entering mechanisms of nanoparticles in the respiratory tract
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Swiss Med Wkly 138:387-91. 2008
    ....
  30. pmc Re-evaluation of pulmonary titanium dioxide nanoparticle distribution using the "relative deposition index": Evidence for clearance through microvasculature
    Christian Mühlfeld
    Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Part Fibre Toxicol 4:7. 2007
    ..abstract:..
  31. doi Effects of combustion-derived ultrafine particles and manufactured nanoparticles on heart cells in vitro
    Maria Helfenstein
    Institute for Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    Toxicology 253:70-8. 2008
    ..These results suggest that diesel exhaust as well as titanium dioxide particles and to a lesser extent also single-walled carbon nanotubes can directly induce cardiac cell damage and can affect the function of the cells...
  32. ncbi Role of dendritic cells in the lung: in vitro models, animal models and human studies
    Fabian Blank
    Institute of Anatomy, Division of Histology, University of Berne, Baltzerstrasse 2, CH 3000 Berne 9, Switzerland
    Expert Rev Respir Med 2:215-33. 2008
    ..Furthermore, we present a number of up-to-date visualization techniques to characterize DC-related cell interactions in vitro and/or in vivo...
  33. pmc Exovesicles from human activated dendritic cells fuse with resting dendritic cells, allowing them to present alloantigens
    Carolina Obregon
    Department of Clinical Research, Institute of Anatomy, University of Bern, and Clinic and Policlinic of Pneumology, University Hospital of Bern, Freiburgstr 15, CH 3010 Berne, Switzerland
    Am J Pathol 169:2127-36. 2006
    ..We concluded that, within a few hours from their release, exovesicles may amplify local or distant adaptive immunological response...
  34. ncbi A novel quantitative method for analyzing the distributions of nanoparticles between different tissue and intracellular compartments
    Christian Mühlfeld
    University of Bern, Institute of Anatomy, Division of Histology, Baltzerstrasse 2, CH 3000 Bern 9, Switzerland
    J Aerosol Med 20:395-407. 2007
    ..We first describe the preconditions and the way to implement these methods, then provide three worked examples, and finally discuss the advantages, pitfalls, and limitations of this method...
  35. pmc Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance
    Megan L McCain
    Department of Physiology, University of Bern, Bern, Switzerland
    Am J Physiol Heart Circ Physiol 302:H443-50. 2012
    ..Our results show a linear correlation between g(j) and Cx43 immunosignal within a range of 8-50 nS...
  36. ncbi Targeting her-2/neu with antirat Neu virosomes for cancer therapy
    Ernst Waelti
    Department of Clinical Research, University of Bern, 3010 Bern, Switzerland
    Cancer Res 62:437-44. 2002
    ..These results indicate that Vir conjugated with an antibody against a tumor antigen are a promising new selective drug delivery system for the treatment of tumors expressing a specific tumor antigen...
  37. ncbi An optimized in vitro model of the respiratory tract wall to study particle cell interactions
    Fabian Blank
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    J Aerosol Med 19:392-405. 2006
    ..In this study, we present an in vitro model of the respiratory tract wall consisting of air-exposed lung epithelial cells covered by a liquid lining layer with a surfactant film to study particle-cell interactions...
  38. ncbi Interaction of fine particles and nanoparticles with red blood cells visualized with advanced microscopic techniques
    Barbara M Rothen-Rutishauser
    Institute of Anatomy, Division of Histology, University of Bern, Bern, Switzerland
    Environ Sci Technol 40:4353-9. 2006
    ..These results suggest that particles may penetrate the red blood cell membrane by a still unknown mechanism different from phagocytosis and endocytosis...
  39. ncbi Fate of inhaled particles after interaction with the lung surface
    Peter Gehr
    Institute of Anatomy, Division of Histology, University of Bern, Baltzerstrasse 2, CH 3000, Bern 9, Switzerland
    Paediatr Respir Rev 7:S73-5. 2006
    ..Furthermore, the cellular response was determined by measurement of TNF-alpha. Dendritic cells gained access to the apical side of the epithelium where they sampled particles and interacted with macrophages...