Research Topics
| Werner Joseph PichlerSummaryAffiliation: University Hospital Country: Switzerland Publications
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Detail Information
Publications
T cells in drug allergyWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, CH 3010 Bern, Switzerland
Curr Allergy Asthma Rep 2:9-15. 2002..Moreover, in MPE CD4+, perforin+ T cells were found in vitro and in immunohistology that had cytotoxic potential and killed keratinocytes in vitro and in vivo...- Immune mechanism of drug hypersensitivityWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, 3010 Bern, Switzerland
Immunol Allergy Clin North Am 24:373-97, v-vi. 2004..Based on recent investigation on different exanthemas, an extended classification of the Gell and Coombs type IV reaction is proposed... - Predictive drug allergy testing: an alternative viewpointW J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology Inselspital, University of Bern, CH 3010, Bern, Switzerland
Toxicology 158:31-41. 2001..A change and expansion of currently performed test procedures is required to predict the allergenic potential of drugs... - Cellular and molecular pathophysiology of cutaneous drug reactionsWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, Bern, Switzerland
Am J Clin Dermatol 3:229-38. 2002.... - Transfection of drug-specific T-cell receptors into hybridoma cells: tools to monitor drug interaction with T-cell receptors and evaluate cross-reactivity to related compoundsDaphné Anne Schmid
MD Division of Allergology, Inselspital, University of Bern, 3010 Bern, Switzerland
Mol Pharmacol 70:356-65. 2006.... - Pharmacological interaction of drugs with antigen-specific immune receptors: the p-i conceptWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, CH 3010 Bern, Switzerland
Curr Opin Allergy Clin Immunol 2:301-5. 2002..In this review a new concept of drug presentation to T-cells is presented... - Modes of presentation of chemical neoantigens to the immune systemWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, CH 3010 Bern, Switzerland
Toxicology 181:49-54. 2002..This seems to be sufficient to stimulate T-cells. Such structural features of a drug have to be considered in the evaluation of drug hypersensitivity reactions and should be taken into account in predictive drug allergy testing... - Lessons from drug allergy: against dogmataWerner J Pichler
Division of Allergology, Clinic of Rheumatology and Clinical Immunology/Allergology, Inselspital, University of Bern, 3010 Bern, Switzerland
Curr Allergy Asthma Rep 3:1-3. 2003 
Immune pathomechanism of drug hypersensitivity reactionsWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, Bern, Switzerland
J Allergy Clin Immunol 127:S74-81. 2011..Among many other presentations, the important role of regulatory T cells in drug hypersensitivity was addressed...- Drug-induced autoimmunityWerner J Pichler
Clinic for Rheumatology, University of Bern, Bern, Switzerland
Curr Opin Allergy Clin Immunol 3:249-53. 2003..Presentation of different mechanism of drug-induced autoimmunity and highlighting of new developments... - Delayed drug hypersensitivity reactionsWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, Bern, Switzerland
Ann Intern Med 139:683-93. 2003..Moreover, cytotoxic functions by either CD4+ or CD8+ T cells (type IVc) seem to participate in all type IV reactions... - Pharmacological interaction of drugs with immune receptors: the p-i conceptWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspitel, Berne, Switzerland
Allergol Int 55:17-25. 2006.... - Direct T-cell stimulations by drugs--bypassing the innate immune systemWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Berne, CH 3010 Berne, Switzerland
Toxicology 209:95-100. 2005..This concept has major implications for preclinical testing of the allergenic potential of a drug, which normally is measured by the ability of the drug to cause a new immune response... - [Intolerance as consequence of hyperreactivity]W J Pichler
Universitätsklinik für Rheumatologie and klinische Immunologie Allergologie, Universitatsspital, Inselspital, Bern
Praxis (Bern 1994) 98:389-94. 2009..Four examples are presented where this cause--trigger model of hyperreactivity is illustrated (exercise induced asthma, pollen associated food allergy, flare-up reactions in drug allergy and hyperreactivity in chronic urticaria)... - Drug hypersensitivity reactions: pathomechanism and clinical symptomsWerner J Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, CH 3010 Bern, Switzerland
Med Clin North Am 94:645-64, xv. 2010..Based on progress in T cell immunology, this heterogeneity of T cell reaction is now also reflected in a subclassification of type IVa to IVd reactions... - Predicting drug hypersensitivity by in vitro testsWerner J Pichler
Department for Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, Bern, Switzerland
ALTEX 24:49-52. 2007..It is frequent in systemic drug hypersensitivity reactions and has major implications for predicting them. First experiments to identify such drugs able to interact with T cells are presented... - Drug interaction with T-cell receptors: T-cell receptor density determines degree of cross-reactivityJan Paul Heribert Depta
Division of Allergology, Clinic of Rheumatology and Clinical Immunology/Allergology, Inselspital, University of Bern, Bern, Switzerland
J Allergy Clin Immunol 113:519-27. 2004..Stably transfected T-cell hybridomas may represent a powerful screening tool for cross-reactivity of newly generated sulfonamide-containing compounds such as celecoxib... - Robust expression of CCR3 as a single basophil selection marker in flow cytometryO V Hausmann
Department of Rheumatology, Clinical Immunology and Allergology, Inselspital, University Bern, Bern, Switzerland
Allergy 66:85-91. 2011..Whereas activation markers, especially CD63, are widely validated, the most suitable and robust marker for basophil selection is still a matter of debate... - Non-covalent presentation of sulfamethoxazole to human CD4+ T cells is independent of distinct human leucocyte antigen-bound peptidesC Burkhart
Clinic of Rheumatology and Clinical Immunology/Allergology, Inselspital, Bern, Switzerland
Clin Exp Allergy 32:1635-43. 2002..CONCLUSION: Our data suggest that small molecules like drugs can bind to a multitude of HLA-bound peptides or that, similar to superantigens, they might bind directly to HLA... - Incidence of anaphylaxis with circulatory symptoms: a study over a 3-year period comprising 940,000 inhabitants of the Swiss Canton BernA Helbling
Division of Allergology, Department of Rheumatology, Immunology and Allergology, University Hospital Bern, Switzerland
Clin Exp Allergy 34:285-90. 2004..However, data on the epidemiology of anaphylaxis, particularly the most profound and life-threatening form such as anaphylactic shock are limited and thought to be under-reported... - Boletus edulis: a digestion-resistant allergen may be relevant for food allergyA Helbling
Division of Allergology, Department of Rheumathology and Clinical Immunology Allergology, University Hospital, 3010 Bern, Switzerland
Clin Exp Allergy 32:771-5. 2002..Reports of food-related allergic reactions due to the edible mushroom Boletus edulis have occasionally been reported... 
Influence of reduced glutathione on the proliferative response of sulfamethoxazole-specific and sulfamethoxazole-metabolite-specific human CD4+ T-cellsC Burkhart
Clinic of Rheumatology and Clinical Immunology Allergology, Inselspital, 3010 Bern, Switzerland
Br J Pharmacol 132:623-30. 2001..4. Our data support the concept that in allergic individuals T-cells recognize the non-covalently bound parent drug rather than APC covalently modified by SMX-NO...- Hypersensitivity reactions to quinolonesDaphné Anne Schmid
Division of Allergology, Clinic of Rheumatology and Clinical Immunology Allergology, Inselspital, University of Bern, Bern, Switzerland
Curr Pharm Des 12:3313-26. 2006..This review gives an insight into the different immunological mechanisms leading to the diverse symptoms of quinolone-induced hypersensitivity reactions, with special emphasis on the role of T cells in such reactions... - T cell involvement in cutaneous drug eruptionsY Hari
Allergy Division of the Clinic for Rheumatology and Clinical Immunology/Allergology, Inselspital, Bern, Switzerland
Clin Exp Allergy 31:1398-408. 2001..A predominant CD8(+) T cell activation leads to more severe (bullous) skin symptoms or liver involvement, while predominant activation of CD4(+) cells elicits mainly maculo-papular reactions... - Effect of tree pollen specific, subcutaneous immunotherapy on the oral allergy syndrome to apple and hazelnutX Bucher
Division of Allergology, Department of Rheumatology and Clinical Immunology/Allergology, Bern, Switzerland
Allergy 59:1272-6. 2004..In spite of this outcome, the amount of apple/hazelnut tolerated is still small. Thus, the effect of SIT on the patients' management of OAS remains limited... - T-cell reactions to drugs in distinct clinical manifestations of drug allergyC B Neukomm
Division of Allergology, Clinic of Rheumatology and Clinical Immunology/Allergology, Inselspital, Bern, Switzerland
J Investig Allergol Clin Immunol 11:275-84. 2001..A combined approach using a detailed case history, lymphocyte transformation tests, skin tests (immediate and delayed type) appears to be helpful to identifying the incriminated drug... - Delayed drug hypersensitivity reactions - new conceptsS J Posadas
Division Allergology, Clinic for Rheumatology and Clinical Immunology Allergology, Bern, Switzerland
Clin Exp Allergy 37:989-99. 2007..This new concept has major implications for understanding clinical and immunological features of drug hypersensitivity and a model to explain the frequent skin symptoms in drug hypersensitivity is proposed... - CD69 upregulation on T cells as an in vitro marker for delayed-type drug hypersensitivityA Beeler
Division of Allergology, Clinic of Rheumatology and Clinical Immunology Allergology, Inselspital, Bern, Switzerland
Allergy 63:181-8. 2008..However, the LTT imposes limitations in terms of practicability, and an alternative method that is easier to implement than the LTT would be desirable... - Multiple drug hypersensitivity--proof of multiple drug hypersensitivity by patch and lymphocyte transformation testsC Gex-Collet
Division of Allergology, Department of Rheumatology and Clinical Immunology/Allergology, Inselspital Bern, Switzerland
J Investig Allergol Clin Immunol 15:293-6. 2005..Antibiotics are often involved, but we also found sensitization to antiepileptics, hypnotics, antidepressants, local anesthetics, corticosteroids and other drug classes... - T cell-mediated hypersensitivity to quinolones: mechanisms and cross-reactivityD A Schmid
Division of Allergology, Clinic of Rheumatology and Clinical Immunology Allergology, University of Bern, Bern, Switzerland
Clin Exp Allergy 36:59-69. 2006..Quinolones are widely used, broad spectrum antibiotics that can induce immediate- and delayed-type hypersensitivity reactions, presumably either IgE or T cell mediated, in about 2-3% of treated patients... - T-cell involvement in drug-induced acute generalized exanthematous pustulosisM Britschgi
Clinic of Rheumatology and Clinical Immunology/Allergology, Inselspital, Bern, Switzerland
J Clin Invest 107:1433-41. 2001..In addition, they indicate that even in some neutrophil-rich inflammatory responses specific T cells are engaged and might orchestrate the immune reaction... - Degeneracy and additional alloreactivity of drug-specific human alpha beta(+) T cell clonesS von Greyerz
Allergology, Clinic for Rheumatology and Clinical Immunology/Allergology, Inselspital, 3010 Bern, Switzerland
Int Immunol 13:877-85. 2001..Finally, the biological functions of bispecific TCC were not influenced by the nature of the stimulating ligand. Both drug as well as allogeneic stimulation led to similar reaction patterns in the analyzed TCC... - The lymphocyte transformation test in the diagnosis of drug hypersensitivityW J Pichler
Division of Allergology, Clinic of Rheumatology and Clinical Immunology/Allergology, Inselspital, University of Bern, 3010-Bern, Switzerland
Allergy 59:809-20. 2004..Further work on the simplification of this test and systematic evaluation of its sensitivity and specificity in some main groups of drugs are necessary to make this test more widely available... - Three years of specific immunotherapy with house-dust-mite extracts in patients with rhinitis and asthma: significant improvement of allergen-specific parameters and of nonspecific bronchial hyperreactivityC E Pichler
Division of Allergology, Clinic for Rheumatology and Clinical Immunology/Allergology, University Hospital, Bern, Switzerland
Allergy 56:301-6. 2001..This beneficial effect of SIT on allergen-specific immune parameters seems to induce also a diminution of nonspecific bronchial hyperreactivity and enables reduction of symptomatic treatment... - Effect of natural seasonal pollen exposure and repeated nasal allergen provocations on elevation of exhaled nitric oxideK Bergmann-Hug
Department of Rheumatology and Clinical Immunology Allergology, Division of Allergology, Inselspital, University of Bern, Bern, Switzerland
Allergy 64:1629-34. 2009..We wondered whether in patients with intermittent allergic rhinitis only (i) natural pollen exposure and (ii) artificial pollen exposure by repeated nasal allergen provocations may lead to an elevation of FENO... - [Specific and nonspecific (anti-IgE) immunotherapy of allergic diseases]W J Pichler
Abteilung Allergologie, , Inselspital, Bern
Ther Umsch 58:329-36. 2001..e. patients suffering from mite, fungal and pollen allergy) or is effective in patients which have manifestations of their allergy in different organs... - Drug-specific in vitro release of IL-2, IL-5, IL-13 and IFN-gamma in patients with delayed-type drug hypersensitivityP Lochmatter
Clinic of Rheumatology and Clinical Immunology Allergology, Inselspital, Bern University Hospital, University of Bern, Switzerland
Allergy 64:1269-78. 2009.... - Zafirlukast has no beneficial effects in the treatment of chronic urticariaA Reimers
Medial Division, Zieglerspital Bern, Bern, Switzerland
Clin Exp Allergy 32:1763-8. 2002..Indeed, various reports have indicated a beneficial effect for leukotriene receptor antagonists in patients with chronic urticaria... - Cytotoxic mechanisms in different forms of T-cell-mediated drug allergiesP C Kuechler
Clinic of Rheumatology and Clinical Immunology/Allergology, PKT 2 D572, Inselspital/University Hospital, CH-3010 Berne, Switzerland
Allergy 59:613-22. 2004..CONCLUSION: Our data underline the important role of cytotoxic mechanisms in different forms of drug induced exanthems and suggest that even some T cells with NK-like characteristics may be involved in drug hypersensitivity... - The butterbur extract petasin has no effect on skin test reactivity induced by different stimuli: a randomized, double-blind crossover study using histamine, codeine, methacholine, and aeroallergen solutionsC Gex-Collet
Division of Allergology, Department of Rheumatology and Clinical Immunology Allergology, Inselspital, Bern, Switzerland
J Investig Allergol Clin Immunol 16:156-61. 2006..Few clinical studies have been performed so far addressing the clinical effectiveness of Ze 339... - Scratch-patch and patch testing in drug allergy--an assessment of specificityK Hug
Division of Allergology, Clinic for Rheumatology and Clinical Immunology/Allergology, University of Bern, Inselspital, Bern, Switzerland
J Investig Allergol Clin Immunol 13:12-9. 2003..Certain drugs solved in PBS are irritant over a wide dosage range and need to be applied in petrolatum... - Adverse side-effects to biological agentsW J Pichler
Department for Rheumatology and Clinical Immunology/Allergology, Inselspital, University of Bern, Bern, Switzerland
Allergy 61:912-20. 2006..This classification could help to better deal with the clinical features of these side-effects, to identify possible individual and general risk factors and to direct research in this novel area of medicine... - Processing-dependent and -independent pathways for recognition of iodinated contrast media by specific human T cellsM Keller
Adverse Drug Reactions Analysis and Consulting ADR AC GmbH, Bern, Switzerland
Clin Exp Allergy 40:257-68. 2010..We have previously demonstrated that CM-specific T cell clones (TCCs) show a broad range of cross-reactivity to different CM. However, the mechanism of specific CM recognition by T cell receptors (TCRs) has not been analysed so far... - Pathogenesis of drug-induced exanthemaN Yawalkar
Clinic for Rheumatology and Clinical Immunology/Allergology, University of Bern, Switzerland
Int Arch Allergy Immunol 124:336-8. 2001.... - Oral prednisolone induced acute generalized exanthematous pustulosis due to corticosteroids of group A confirmed by epicutaneous testing and lymphocyte transformation testsU Buettiker
Department of Dermatology, Inselspital, University of Berne, Berne, Switzerland
Dermatology 213:40-3. 2006..Our data support previous reports indicating an important role for drug-specific T cells in inducing neutrophil inflammation in this disease... - In vitro detection of cytotoxic T and NK cells in peripheral blood of patients with various drug-induced skin diseasesA Zawodniak
Division of Allergology, Clinic for Rheumatology and Clinical Immunology Allergology Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland
Allergy 65:376-84. 2010....