Erich A Nigg

Summary

Affiliation: University of Basel
Country: Switzerland

Publications

  1. pmc 3D-structured illumination microscopy provides novel insight into architecture of human centrosomes
    Katharina F Sonnen
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Biol Open 1:965-76. 2012
  2. pmc On the regulation, function, and localization of the DNA-dependent ATPase PICH
    Manuel Kaulich
    Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50 70, 4065 Basel, Switzerland
    Chromosoma 121:395-408. 2012
  3. pmc Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3
    Thomas N Gaitanos
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    EMBO J 28:1442-52. 2009
  4. pmc Re-examination of siRNA specificity questions role of PICH and Tao1 in the spindle checkpoint and identifies Mad2 as a sensitive target for small RNAs
    Nadja C Hübner
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Munich, Germany
    Chromosoma 119:149-65. 2010
  5. doi request reprint Centrioles, centrosomes, and cilia in health and disease
    Erich A Nigg
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Cell 139:663-78. 2009
  6. doi request reprint The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
    Erich A Nigg
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Nat Cell Biol 13:1154-60. 2011
  7. ncbi request reprint Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1
    Rüdiger Neef
    Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
    Nat Cell Biol 9:436-44. 2007
  8. pmc The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles
    Giulia Guarguaglini
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152, Martinsried, Germany
    Mol Biol Cell 16:1095-107. 2005
  9. ncbi request reprint Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 131:271-85. 2007
  10. pmc Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    J Cell Biol 166:167-72. 2004

Collaborators

Detail Information

Publications70

  1. pmc 3D-structured illumination microscopy provides novel insight into architecture of human centrosomes
    Katharina F Sonnen
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Biol Open 1:965-76. 2012
    ..Our study provides novel insights into the architecture of human centrosomes and illustrates the power of super-resolution microscopy in revealing the relative localization of centriole and PCM proteins in unprecedented detail...
  2. pmc On the regulation, function, and localization of the DNA-dependent ATPase PICH
    Manuel Kaulich
    Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50 70, 4065 Basel, Switzerland
    Chromosoma 121:395-408. 2012
    ..Although not required for the spindle assembly checkpoint, PICH is clearly important for faithful chromosome segregation...
  3. pmc Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3
    Thomas N Gaitanos
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    EMBO J 28:1442-52. 2009
    ..We propose that the Ska complex functionally complements the KMN, providing an additional layer of stability to KT-MT attachment and possibly signalling completion of attachment to the spindle checkpoint...
  4. pmc Re-examination of siRNA specificity questions role of PICH and Tao1 in the spindle checkpoint and identifies Mad2 as a sensitive target for small RNAs
    Nadja C Hübner
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Munich, Germany
    Chromosoma 119:149-65. 2010
    ..In support of the latter conclusion, our evidence suggests that an off-target effect on Mad2 may also contribute to explain the apparent role of the Tao1 kinase in SAC signaling...
  5. doi request reprint Centrioles, centrosomes, and cilia in health and disease
    Erich A Nigg
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Cell 139:663-78. 2009
    ..Finally, structural or functional centriole aberrations contribute to ciliopathies, a variety of complex diseases that stem from the absence or dysfunction of cilia...
  6. doi request reprint The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
    Erich A Nigg
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Nat Cell Biol 13:1154-60. 2011
    ..We discuss spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of centrioles and centrosomes...
  7. ncbi request reprint Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1
    Rüdiger Neef
    Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
    Nat Cell Biol 9:436-44. 2007
    ..The activation state of Cdk1, therefore, controls the switch of Plk1 localization from centrosomes and kinetochores during metaphase, to the central spindle during anaphase...
  8. pmc The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles
    Giulia Guarguaglini
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152, Martinsried, Germany
    Mol Biol Cell 16:1095-107. 2005
    ..We show that Cep170 labeling can be used to discriminate bona fide centriole overduplication from centriole amplification that results from aborted cell division...
  9. ncbi request reprint Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 131:271-85. 2007
    ..Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization...
  10. pmc Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    J Cell Biol 166:167-72. 2004
    ..We propose that MKlp2 is involved in the localization of Plk1, Aurora B, and Cdc14A to the central spindle during anaphase, and that the integration of signaling by these proteins is necessary for proper cytokinesis...
  11. ncbi request reprint Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2
    Silvia Martin-Lluesma
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Science 297:2267-70. 2002
    ..Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint-deficient cells...
  12. ncbi request reprint PICH, a centromere-associated SNF2 family ATPase, is regulated by Plk1 and required for the spindle checkpoint
    Christoph Baumann
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 128:101-14. 2007
    ..These data identify PICH as a novel essential component of checkpoint signaling. We propose that PICH binds to catenated centromere-related DNA to monitor tension developing between sister kinetochores...
  13. pmc The Plk1-dependent phosphoproteome of the early mitotic spindle
    Anna Santamaria
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Mol Cell Proteomics 10:M110.004457. 2011
    ....
  14. pmc KIF14 and citron kinase act together to promote efficient cytokinesis
    Ulrike Gruneberg
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Biol 172:363-72. 2006
    ..Atzori, E. Turco, R. Triolo, G.P. Dotto, et al. 2000. Neuron. 28:115-127), we find a general requirement for citron kinase in human cell division. Together, these findings identify a novel pathway required for efficient cytokinesis...
  15. pmc Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction
    Ying Wai Chan
    Growth and Development, Biozentrum, University of Basel, 4056 Basel, Switzerland
    J Cell Biol 196:563-71. 2012
    ..We propose that Aurora B phosphorylation antagonizes the interaction between the Ska complex and the KMN network, thereby controlling Ska recruitment to KTs and stabilization of KT-MT attachments...
  16. ncbi request reprint Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome
    Martina Casenghi
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 118:5101-8. 2005
    ..These findings uncover a mechanism through which Plk1 helps to coordinate changes in MT organisation with cell cycle progression, by controlling the dynein-dynactin-dependent transport of centrosomal proteins...
  17. pmc Human TPX2 is required for targeting Aurora-A kinase to the spindle
    Thomas A Kufer
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Cell Biol 158:617-23. 2002
    ..We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly...
  18. pmc Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint
    Luca L Fava
    Growth and Development, Biozentrum, University of Basel, Basel, Switzerland
    EMBO J 30:3322-36. 2011
    ..Our study provides direct in vivo evidence for the model that a kinetochore complex of Mad1:C-Mad2 acts as a template to sustain the SAC and it challenges the distinction between SAC and mitotic timer...
  19. pmc Different Plk1 functions show distinct dependencies on Polo-Box domain-mediated targeting
    Anja Hanisch
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 17:448-59. 2006
    ..This opens the possibility that PBD-directed drugs might be developed to selectively interfere with a subset of Plk1 functions...
  20. pmc Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
    Rüdiger Neef
    Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Biol 162:863-75. 2003
    ..We propose that phosphorylation of MKlp2 by Plk1 is necessary for the spatial restriction of Plk1 to the central spindle during anaphase and telophase, and the complex of these two proteins is required for cytokinesis...
  21. doi request reprint Plk4 trans-autophosphorylation regulates centriole number by controlling betaTrCP-mediated degradation
    Gernot Guderian
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 123:2163-9. 2010
    ..We conclude that active Plk4 promotes its own degradation by catalyzing betaTrCP binding through trans-autophosphorylation (phosphorylation by the other kinase in the dimer) within homodimers...
  22. doi request reprint Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
    ..In particular, nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylation site occupancy in mitosis. This suggests that these proteins may be inactivated by phosphorylation in mitotic cells...
  23. pmc Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly
    Ying Wai Chan
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 185:859-74. 2009
    ..Collectively, our data reveal hSpindly-mediated dynein functions and highlight a critical role of KT dynein in spindle orientation...
  24. ncbi request reprint Regulation of Aurora-A kinase on the mitotic spindle
    Thomas A Kufer
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany
    Chromosoma 112:159-63. 2003
    ..Here, we review these findings with particular emphasis on the role of TPX2, a prominent spindle component implicated in a Ran-GTP-mediated spindle assembly pathway...
  25. pmc Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP
    Ulf R Klein
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Mol Biol Cell 17:2547-58. 2006
    ..Our findings thus establish a functional module within the CPC that assembles on the N terminus of INCENP and controls centromere recruitment...
  26. pmc Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis
    Anna Santamaria
    Department of Cell Biology and Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
    Mol Biol Cell 18:4024-36. 2007
    ..Finally, we show that Plk1 activity is essential for cleavage furrow formation and ingression, leading to successful cytokinesis...
  27. ncbi request reprint Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation
    Martina Casenghi
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Dev Cell 5:113-25. 2003
    ....
  28. doi request reprint Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1
    Sabine Elowe
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    J Cell Sci 123:84-94. 2010
    ....
  29. pmc Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions
    Sabine Elowe
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Genes Dev 21:2205-19. 2007
    ....
  30. ncbi request reprint Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion
    Susanne Graser
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Cell Sci 120:4321-31. 2007
    ..Instead, our data suggest that Cep215 functionally interacts with pericentrin, suggesting that both proteins influence centrosome cohesion through an indirect mechanism related to cytoskeletal dynamics...
  31. doi request reprint Control of centriole length by CPAP and CP110
    Thorsten I Schmidt
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Curr Biol 19:1005-11. 2009
    ..We thus propose that CPAP and CP110 play antagonistic roles in determining the extent of tubulin addition during centriole elongation, thereby controlling the length of newly formed centrioles...
  32. pmc Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity
    Kerstin H Thein
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    J Cell Biol 178:345-54. 2007
    ..We suggest that astrin contributes to the regulatory network that controls separase activity...
  33. pmc Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis
    Reiko Honda
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 14:3325-41. 2003
    ..A nonphosphorylatable mutant (TSS893-895AAA) was a poor activator of Aurora B, demonstrating that INCENP phosphorylation is important for kinase activation...
  34. pmc RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin
    Ulf R Klein
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Mol Biol Cell 20:410-8. 2009
    ..These data thus delineate a mitotic SUMO2/3 conjugation-deconjugation cycle of Borealin and further assign a regulatory function of RanBP2 and SENP3 in the mitotic SUMO pathway...
  35. pmc Plk1 regulates mitotic Aurora A function through betaTrCP-dependent degradation of hBora
    Eunice H Y Chan
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Chromosoma 117:457-69. 2008
    ..This suggests that Plk1 controls Aurora A localization and function by regulating cellular levels of hBora...
  36. ncbi request reprint Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission
    Ravindra B Chalamalasetty
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 119:3008-19. 2006
    ..This failure could be correlated with the persistence of these fragments at structures surrounding the midbody, suggesting that abscission requires the displacement of Ect2 from the contractile ring and its re-import into the nucleus...
  37. doi request reprint Cell-cycle-regulated expression of STIL controls centriole number in human cells
    Christian Arquint
    Biozentrum, University of Basel, Klingelbergstr 50 70, CH 4056 Basel, Switzerland
    J Cell Sci 125:1342-52. 2012
    ..Thus, STIL cooperates with SAS-6 and PLK4 in the control of centriole number and represents a key centriole duplication factor in human cells...
  38. pmc Persistence of DNA threads in human anaphase cells suggests late completion of sister chromatid decatenation
    Lily Hui Ching Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Chromosoma 117:123-35. 2008
    ..Moreover, they show that topoisomerase activity is required during anaphase for the resolution of PICH-positive threads, implying that the complete separation of sister chromatids occurs later than previously assumed...
  39. pmc Cep164, a novel centriole appendage protein required for primary cilium formation
    Susanne Graser
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried D 82152, Germany
    J Cell Biol 179:321-30. 2007
    ..Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures...
  40. ncbi request reprint Calcium triggers exit from meiosis II by targeting the APC/C inhibitor XErp1 for degradation
    Nadine R Rauh
    Chemical Biology, Independent Research Group, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nature 437:1048-52. 2005
    ..These results provide a molecular explanation for how the fertilization-induced calcium increase triggers exit from meiosis II...
  41. ncbi request reprint The Polo kinase Plk4 functions in centriole duplication
    Robert Habedanck
    Department of Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Cell Biol 7:1140-6. 2005
    ..These findings provide an attractive explanation for the crucial function of Plk4 in cell proliferation and have implications for the role of Polo kinases in tumorigenesis...
  42. ncbi request reprint Plk4-induced centriole biogenesis in human cells
    Julia Kleylein-Sohn
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Dev Cell 13:190-202. 2007
    ..Collectively, these data afford a comprehensive view of the assembly pathway underlying centriole biogenesis in human cells...
  43. doi request reprint Structural and functional organization of the Ska complex, a key component of the kinetochore-microtubule interface
    A Arockia Jeyaprakash
    Max Planck Institute of Biochemistry, Department of Structural Cell Biology, Martinsried, Germany
    Mol Cell 46:274-86. 2012
    ..We discuss how this symmetric architecture might complement and stabilize the Ndc80-microtubule attachments with analogies to the yeast Dam1/DASH complex...
  44. pmc Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2
    Anja Hanisch
    Department of Cell Biology, Max Planck Institute for Biochemistry, Martinsried, Germany
    EMBO J 25:5504-15. 2006
    ..These data suggest that the Ska1/2 complex plays a critical role in the maintenance of the metaphase plate and/or spindle checkpoint silencing...
  45. doi request reprint Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages
    Rainer Malik
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Proteome Res 8:4553-63. 2009
    ..Taken together, our results constitute a large quantitative data resource of phosphorylation abundances at distinct mitotic stages and they provide insight into the systems properties of phosphorylation dynamics during mitosis...
  46. pmc A complex of two centrosomal proteins, CAP350 and FOP, cooperates with EB1 in microtubule anchoring
    Xiumin Yan
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Mol Biol Cell 17:634-44. 2006
    ..These results have implications for the mechanisms underlying MT anchoring at the centrosome and they attribute a key MT anchoring function to two novel centrosomal proteins, CAP350 and FOP...
  47. doi request reprint SUMO-dependent regulation of centrin-2
    Ulf R Klein
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Sci 122:3312-21. 2009
    ..These data show that the nucleocytoplasmic shuttling of centrin-2 depends on the SUMO system and indicates that localization of centrin-2 within the nucleus depends on its ability to bind to the XPC protein...
  48. ncbi request reprint Cytostatic factor: an activity that puts the cell cycle on hold
    Andreas Schmidt
    Chemical Genetics, Independent Research Group, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Cell Sci 119:1213-8. 2006
    ..Most importantly, studies on Erp1/Emi2 regulation have led to a detailed molecular understanding of the Ca2+-mediated release from CSF arrest that occurs upon fertilization...
  49. pmc Quantitative mass spectrometry analysis reveals similar substrate consensus motif for human Mps1 kinase and Plk1
    Zhen Dou
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    PLoS ONE 6:e18793. 2011
    ..Human Mps1 (hMps1) is highly phosphorylated during mitosis and many phosphorylation sites have been identified. However, the upstream kinases responsible for these phosphorylations are not presently known...
  50. ncbi request reprint Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1
    Volker M Stucke
    Department of Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Chromosoma 113:1-15. 2004
    ..Our data indicate that the catalytic domain of hMps1 displays affinity for microtubules and that microtubule binding could contribute to the regulation of kinase activity...
  51. doi request reprint The spindle protein CHICA mediates localization of the chromokinesin Kid to the mitotic spindle
    Anna Santamaria
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Curr Biol 18:723-9. 2008
    ..We conclude that CHICA represents a novel interaction partner of the chromokinesin Kid that is required for the generation of polar ejection forces and chromosome congression...
  52. doi request reprint Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division
    Lily Hui Ching Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Sci 123:806-13. 2010
    ..Our data suggest that centromere decatenation can occur only after separase activation and cohesin removal, providing a plausible explanation for the persistence of centromere threads after anaphase onset...
  53. pmc Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity
    Andreas Schmidt
    Chemical Biology, Independent Research Group, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Genes Dev 19:502-13. 2005
    ..Plx1 phosphorylates XErp1 in vitro at a site that targets XErp1 for degradation upon CSF release. Thus, our data lead to a model of APC/C activation in Xenopus egg extract in which Plx1 targets the APC/C inhibitor XErp1 for degradation...
  54. pmc Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication
    Volker M Stucke
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 21:1723-32. 2002
    ..In contrast, centrosome (re-)duplication as well as cell division occur in the absence of hMps1. We conclude that hMps1 is required for the spindle assembly checkpoint but not for centrosome duplication...
  55. ncbi request reprint Signal transduction. Capturing polo kinase
    Herman H W Sillje
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Science 299:1190-1. 2003
  56. pmc Rootletin forms centriole-associated filaments and functions in centrosome cohesion
    Susanne Bahe
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 171:27-33. 2005
    ..The ability of rootletin to form centriole-associated fibers suggests a dynamic model for centrosome cohesion based on entangling filaments rather than continuous polymeric linkers...
  57. ncbi request reprint HURP is a Ran-importin beta-regulated protein that stabilizes kinetochore microtubules in the vicinity of chromosomes
    Herman H W Sillje
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Curr Biol 16:731-42. 2006
    ..How RanGTP regulates kinetochore-microtubule (K-fiber) formation is not presently understood...
  58. doi request reprint Human Cep192 and Cep152 cooperate in Plk4 recruitment and centriole duplication
    Katharina F Sonnen
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, 4056 Basel, Switzerland
    J Cell Sci 126:3223-33. 2013
    ..We conclude that cooperation between Cep192 and Cep152 is crucial for centriole recruitment of Plk4 and centriole duplication during the cell cycle...
  59. ncbi request reprint Structure of the N-terminal domain of the FOP (FGFR1OP) protein and implications for its dimerization and centrosomal localization
    Aleksandra Mikolajka
    Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Mol Biol 359:863-75. 2006
    ..The central part of the dimer contains the LisH domain. We further determined that the FOP LisH domain is part of a longer N-terminal segment that is required, albeit not sufficient, for dimerization and centrosomal localization of FOP...
  60. ncbi request reprint The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles
    Thibault Mayor
    Department of Cell Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Cell Sci 115:3275-84. 2002
    ..We conclude that the dissociation of C-Nap1 from mitotic centrosomes is regulated by localized phosphorylation rather than generalized proteolysis...
  61. doi request reprint Comparative conservation analysis of the human mitotic phosphoproteome
    Rainer Malik
    Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried D 82152, Germany
    Bioinformatics 24:1426-32. 2008
    ....
  62. ncbi request reprint Polo-like kinases and the orchestration of cell division
    Francis A Barr
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 5:429-40. 2004
  63. doi request reprint From proteome lists to biological impact--tools and strategies for the analysis of large MS data sets
    Rainer Malik
    Max Planck Institute of Biochemistry, Department of Cell Biology, Martinsried, Germany
    Proteomics 10:1270-83. 2010
    ..We expect that these types of analyses will significantly contribute to a deeper understanding of the role of individual proteins, protein networks and pathways in complex systems...
  64. pmc Phosphoproteome analysis of the human mitotic spindle
    Marjaana Nousiainen
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 103:5391-6. 2006
    ..This inventory of spindle phosphorylation sites should thus make an important contribution to a better understanding of the molecular mechanisms that regulate the formation, function, and integrity of the mitotic spindle...
  65. pmc Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression
    Kalyan Dulla
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell Proteomics 9:1167-81. 2010
    ..Thus, the results of this study provide a valuable resource for cell biologists and provide insight into the system properties of the mitotic phosphokinome...
  66. doi request reprint Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis
    Bin Wang
    Department of Cell Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    Anal Chem 80:9526-33. 2008
    ..Approximately 30% of the phosphorylation sites were exclusively identified after digestion by elastase, demonstrating the value of this enzyme for phosphoproteome studies...
  67. ncbi request reprint Centrosome aberrations: cause or consequence of cancer progression?
    Erich A Nigg
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopfersitz 18a, D 82152 Martinsried, Germany
    Nat Rev Cancer 2:815-25. 2002
  68. ncbi request reprint Centrosome duplication: of rules and licenses
    Erich A Nigg
    Max Planck Institute for Biochemistry, Am Klopferspitz 18, Martinsried, Germany
    Trends Cell Biol 17:215-21. 2007
    ..Finally, Plk4 (also called Sak), a member of the Polo kinase family, has been identified as a novel positive regulator of centriole formation...
  69. ncbi request reprint Purification of mitotic spindles from cultured human cells
    Herman H W Sillje
    Max Planck Institute of Biochemistry, Department of Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Methods 38:25-8. 2006
    ..Sauer, R. Korner, A. Hanisch, A. Ries, E.A. Nigg, H.H.W. Sillje, Mol. Cell. Proteomics 4 (2005) 35-43]...
  70. doi request reprint STIL microcephaly mutations interfere with APC/C-mediated degradation and cause centriole amplification
    Christian Arquint
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, 4056 Basel, Switzerland
    Curr Biol 24:351-60. 2014
    ..Excess STIL triggers centriole amplification, raising the question of how STIL levels are regulated...