E A Nigg

Summary

Affiliation: University of Geneva
Country: Switzerland

Publications

  1. ncbi request reprint Nucleocytoplasmic transport: signals, mechanisms and regulation
    E A Nigg
    Department of Molecular Biology, Sciences II, University of Geneva, Switzerland
    Nature 386:779-87. 1997
  2. ncbi request reprint Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation
    E A Nigg
    Department of Molecular Biology, Sciences II, University of Geneva, 30, Quai Ernest Ansermet, Geneva 4, CH 1211, Switzerland
    Exp Cell Res 229:174-80. 1996
  3. pmc The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion
    T Mayor
    Department of Molecular Biology, Sciences II, University of Geneva, CH 1211 Geneva, Switzerland
    J Cell Biol 151:837-46. 2000
  4. pmc C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, CH 1211 Geneva 4, Switzerland
    J Cell Biol 141:1563-74. 1998
  5. ncbi request reprint Protein kinases in control of the centrosome cycle
    T Mayor
    Department of Molecular Biology, University of Geneva, Switzerland
    FEBS Lett 452:92-5. 1999
  6. pmc Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication
    H H Silljé
    Department of Molecular Biology, Sciences II, 30 Quai Ernest Ansermet, University of Geneva, CH 1211 Geneva 4, Switzerland
    EMBO J 18:5691-702. 1999
  7. ncbi request reprint Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators
    K Tanaka
    Department of Molecular Biology, Sciences II, University of Geneva 30, Quai Ernest Ansermet, 1211 Geneve 4, Switzerland
    J Biol Chem 274:13491-7. 1999
  8. ncbi request reprint Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, 30, Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
    J Biol Chem 274:16304-10. 1999
  9. pmc A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, Switzerland
    EMBO J 17:470-81. 1998
  10. ncbi request reprint Polo-like kinases: positive regulators of cell division from start to finish
    E A Nigg
    Department of Molecular Biology University of Geneva Sciences II, 30, quai Ernest Ansermet CH 1211 Geneva 4 Switzerland
    Curr Opin Cell Biol 10:776-83. 1998

Collaborators

  • K Tanaka
  • Andrew M Fry
  • H H Silljé
  • A Blangy
  • H A Lane
  • K Takahashi
  • Yu Liu
  • Jiri Bartek
  • W Krek
  • P Meraldi
  • J P Tassan
  • T Mayor
  • A Duensing
  • S J Schultz
  • Y D Stierhof
  • R M Golsteyn
  • S A Perdreau
  • S Duensing
  • J Kleylein-Sohn
  • V Leclerc
  • J P Adamczewski
  • P Gallant
  • P Leopold
  • M Jaquenoud
  • A Devault
  • G J Hughes
  • S Frutiger
  • T Ried
  • T P Mäkelä
  • M Rossignol
  • P H O'Farrell
  • V Moncollin
  • J M Egly
  • J C Labbe
  • K E Mundt
  • A M Martinez
  • N Morin
  • J C Cavadore
  • M Doree
  • D Fesquet
  • C Sutterlin
  • A Ziemiecki
  • R A Weinberg

Detail Information

Publications33

  1. ncbi request reprint Nucleocytoplasmic transport: signals, mechanisms and regulation
    E A Nigg
    Department of Molecular Biology, Sciences II, University of Geneva, Switzerland
    Nature 386:779-87. 1997
    ..Here I summarize current understanding of nucleocytoplasmic transport and illustrate the importance of regulated transport for signal transduction...
  2. ncbi request reprint Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation
    E A Nigg
    Department of Molecular Biology, Sciences II, University of Geneva, 30, Quai Ernest Ansermet, Geneva 4, CH 1211, Switzerland
    Exp Cell Res 229:174-80. 1996
    ..This observation hints at the existence of a checkpoint linking Cdc2 activation to the presence of functional centrosomes...
  3. pmc The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion
    T Mayor
    Department of Molecular Biology, Sciences II, University of Geneva, CH 1211 Geneva, Switzerland
    J Cell Biol 151:837-46. 2000
    ..We conclude that C-Nap1 is a key component of a dynamic, cell cycle-regulated structure that mediates centriole-centriole cohesion...
  4. pmc C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, CH 1211 Geneva 4, Switzerland
    J Cell Biol 141:1563-74. 1998
    ..Based on these findings, we propose a model implicating both Nek2 and C-Nap1 in the regulation of centriole-centriole cohesion during the cell cycle...
  5. ncbi request reprint Protein kinases in control of the centrosome cycle
    T Mayor
    Department of Molecular Biology, University of Geneva, Switzerland
    FEBS Lett 452:92-5. 1999
    ..Here, we focus on two protein kinases, the polo-like kinase 1 and the NIMA-related kinase 2, for which recent data indicate key roles during the centrosome cycle...
  6. pmc Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication
    H H Silljé
    Department of Molecular Biology, Sciences II, 30 Quai Ernest Ansermet, University of Geneva, CH 1211 Geneva 4, Switzerland
    EMBO J 18:5691-702. 1999
    ..These findings provide the first biochemical clues as to the possible molecular functions of Tlks, a highly conserved family of kinases implicated in the development of multicellular organisms...
  7. ncbi request reprint Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators
    K Tanaka
    Department of Molecular Biology, Sciences II, University of Geneva 30, Quai Ernest Ansermet, 1211 Geneve 4, Switzerland
    J Biol Chem 274:13491-7. 1999
    ....
  8. ncbi request reprint Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, 30, Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
    J Biol Chem 274:16304-10. 1999
    ..Thus, this study reveals not only an important mechanism for the regulation of the Nek2 kinase but, furthermore, highlights an unusual organization of a leucine zipper dimerization motif...
  9. pmc A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators
    A M Fry
    Department of Molecular Biology, Sciences II, University of Geneva, Switzerland
    EMBO J 17:470-81. 1998
    ..Moreover, they indicate that one function of mammalian Nek2 relates to the centrosome cycle and thus provide a new perspective on the role of NIMA-related kinases...
  10. ncbi request reprint Polo-like kinases: positive regulators of cell division from start to finish
    E A Nigg
    Department of Molecular Biology University of Geneva Sciences II, 30, quai Ernest Ansermet CH 1211 Geneva 4 Switzerland
    Curr Opin Cell Biol 10:776-83. 1998
    ..Finally, recent evidence implicates Plks in the temporal and spatial coordination of cytokinesis...
  11. ncbi request reprint Cyclin-dependent kinase 7: at the cross-roads of transcription, DNA repair and cell cycle control?
    E A Nigg
    Department of Molecular Biology, University of Geneva, Sciences II, Switzerland
    Curr Opin Cell Biol 8:312-7. 1996
    ..During the past year, a third subunit associated with CDK7 and cyclin H has been characterized, and the functional link between CDK7 and RNA polymerase II has been strengthened...
  12. ncbi request reprint The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis
    K Tanaka
    Department of Molecular Biology, Sciences II, University of Geneva, Switzerland
    Exp Cell Res 237:264-74. 1997
    ..Taken together, these results indicate that Nek2 may play an important role not only during mitosis but also during meiosis...
  13. ncbi request reprint Centrosome cohesion is regulated by a balance of kinase and phosphatase activities
    P Meraldi
    Department of Cell Biology, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany
    J Cell Sci 114:3749-57. 2001
    ..These studies highlight the importance of protein phosphorylation in the control of centrosome cohesion, and they point to Nek2 and PP1 alpha as critical regulators of centrosome structure...
  14. pmc Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II
    V Leclerc
    URA 671 Centre National de la Recherche Scientifique, Villefranche sur Mer, France
    Mol Biol Cell 7:505-13. 1996
    ..Based on these observations and sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae, we suggest that cyclin C and Cdk8 control RNA polymerase II function...
  15. pmc MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH
    J P Adamczewski
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, UPR 6520 CNRS, Unité 184 INSERM, CU de Strasbourg, France
    EMBO J 15:1877-84. 1996
    ..Although not strictly required for the formation and activity of the complex, it stimulates its kinase activity. The kinase activity of TFIIH, which is constant during the cell cycle, is reduced after UV light irradiation...
  16. pmc In vitro assembly of a functional human CDK7-cyclin H complex requires MAT1, a novel 36 kDa RING finger protein
    J P Tassan
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges, Switzerland
    EMBO J 14:5608-17. 1995
    ..MAT1 constitutes the first example of an assembly factor that appears to be essential for the formation of an active CDK-cyclin complex...
  17. pmc Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function
    R M Golsteyn
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges
    J Cell Biol 129:1617-28. 1995
    ....
  18. pmc Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase)
    J P Tassan
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges
    J Cell Biol 127:467-78. 1994
    ..It suggests that the phosphorylation state of threonine 161 in p34cdc2 (and the corresponding residue in other cdks) may be regulated primarily by the availability of the cdk/cyclin substrates, and by phosphatase(s)...
  19. ncbi request reprint Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5
    R M Golsteyn
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges
    J Cell Sci 107:1509-17. 1994
    ..Thus, like Drosophila polo and S. cerevisiae Cdc5, human Plk1 is likely to function in cell cycle progression...
  20. ncbi request reprint Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases
    H H Silljé
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Curr Biol 11:1068-73. 2001
    ..These data indicate that Tlk family members regulate chromatin assembly during DNA replication, and they suggest a plausible explanation for the pleiotropic developmental defects of plant tousled mutants...
  21. ncbi request reprint Mitotic kinases as regulators of cell division and its checkpoints
    E A Nigg
    Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 2:21-32. 2001
    ..Here, I give an overview of the many mitotic kinases that regulate cell division and the fidelity of chromosome transmission...
  22. ncbi request reprint The centrosome cycle
    P Meraldi
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    FEBS Lett 521:9-13. 2002
    ..Here we summarize recent information on the regulation of the centrosome cycle and its coordination with the chromosomal cell cycle...
  23. pmc Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells
    P Gallant
    Swiss Institute for Experimental Cancer Research ISREC, CH 1066 Epalinges
    J Cell Biol 117:213-24. 1992
    ..Many of the arrested cells displayed multiple mitotic spindles, suggesting that the centrosome cycle had continued in spite of the cell cycle arrest...
  24. pmc Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites
    W Krek
    Swiss Institute for Experimental Cancer Research, ISREC, Epalinges
    EMBO J 10:305-16. 1991
    ....
  25. pmc Structure and developmental expression of the chicken CDC2 kinase
    W Krek
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges s Lausanne
    EMBO J 8:3071-8. 1989
    ..Interestingly, however, when examining individual embryonic tissues, no correlation was observed between levels of cdc2 mRNA and protein, suggesting that cdc2 expression in developing chicken may be regulated at multiple levels...
  26. ncbi request reprint Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans
    S J Schultz
    Swiss Institute for Experimental Cancer Research, Epalinges
    Cell Growth Differ 5:625-35. 1994
    ..These observations demonstrate that Nek2 resembles Aspergillus NIMA, not only in its catalytic domain, but also in its cell cycle-dependent expression. Hence, the human Nek2 protein kinase may also function at the onset of mitosis...
  27. pmc Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C
    J P Tassan
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges
    Proc Natl Acad Sci U S A 92:8871-5. 1995
    ..cerevisiae. Hence, we propose that human K35(CDK8)-cyclin C might be functionally associated with the mammalian transcription apparatus, perhaps involved in relaying growth-regulatory signals...
  28. pmc MAT1 ('menage à trois') a new RING finger protein subunit stabilizing cyclin H-cdk7 complexes in starfish and Xenopus CAK
    A Devault
    Centre de Recherches de Biochimie Macromoleculaire, CNRS UPR 9008, Montpellier, France
    EMBO J 14:5027-36. 1995
    ....
  29. ncbi request reprint A cyclin associated with the CDK-activating kinase MO15
    T P Mäkelä
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Nature 371:254-7. 1994
    ..These findings demonstrate that a cyclin/kinase complex can function as a regulator of other cyclin/kinase complexes, and suggest that cyclin/kinase cascades may exist...
  30. ncbi request reprint Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans
    S J Schultz
    Swiss Institute for Experimental Cancer Research, Epalinges
    Cell Growth Differ 4:821-30. 1993
    ....
  31. pmc Identification of a novel vertebrate cyclin: cyclin B3 shares properties with both A- and B-type cyclins
    P Gallant
    Swiss Institute for Experimental Cancer Research ISREC, Epalinges
    EMBO J 13:595-605. 1994
    ..Based on these observations, we conclude that cyclin B3 may share functional properties with both A- and B-type cyclins...
  32. ncbi request reprint Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo
    A Blangy
    Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland
    Cell 83:1159-69. 1995
    ..These results indicate that HsEg5 is required for establishing a bipolar spindle and that p34cdc2 protein kinase directly regulates its localization...
  33. pmc Centriole overduplication through the concurrent formation of multiple daughter centrioles at single maternal templates
    A Duensing
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Oncogene 26:6280-8. 2007
    ..These results help to explain how oncogenic stimuli can rapidly induce abnormal centriole numbers within a single cell-division cycle and provide insights into the regulation of centriole duplication...