Karin Moelling

Summary

Affiliation: University of Zurich
Country: Switzerland

Publications

  1. ncbi request reprint Regulation of Raf-Akt Cross-talk
    Karin Moelling
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8028 Zurich, Switzerland
    J Biol Chem 277:31099-106. 2002
  2. ncbi request reprint Silencing of HIV by hairpin-loop-structured DNA oligonucleotide
    Karin Moelling
    Institute of Medical Virology, University of Zurich, Switzerland
    FEBS Lett 580:3545-50. 2006
  3. ncbi request reprint Regulation of epithelial wound closure and intercellular adhesion by interaction of AF6 with actin cytoskeleton
    Mihaela Lorger
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    J Cell Sci 119:3385-98. 2006
  4. ncbi request reprint Anti-tumor activity of mesenchymal stem cells producing IL-12 in a mouse melanoma model
    Lina Elzaouk
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Exp Dermatol 15:865-74. 2006
  5. ncbi request reprint WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Czeta
    Thorsten Fritzius
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, Zurich CH 8006, Switzerland
    FEBS J 274:1552-66. 2007
  6. doi request reprint Oligonucleotide-mediated retroviral RNase H activation leads to reduced HIV-1 titer in patient-derived plasma
    Jochen Heinrich
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    AIDS 23:213-21. 2009
  7. ncbi request reprint IP-10-encoding plasmid DNA therapy exhibits anti-tumor and anti-metastatic efficiency
    Johanna Keyser
    Institute of Medical Virology, University of Zurich, Gloriastr, Zurich, Switzerland
    Exp Dermatol 13:380-90. 2004
  8. pmc Akt- and Foxo1-interacting WD-repeat-FYVE protein promotes adipogenesis
    Thorsten Fritzius
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    EMBO J 27:1399-410. 2008
  9. pmc c-Src-mediated epithelial cell migration and invasion regulated by PDZ binding site
    Martin Baumgartner
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    Mol Cell Biol 28:642-55. 2008
  10. doi request reprint The PDZ protein MPP2 interacts with c-Src in epithelial cells
    Martin Baumgartner
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Exp Cell Res 315:2888-98. 2009

Collaborators

Detail Information

Publications48

  1. ncbi request reprint Regulation of Raf-Akt Cross-talk
    Karin Moelling
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8028 Zurich, Switzerland
    J Biol Chem 277:31099-106. 2002
    ..This is highly relevant to understanding cellular transformation and may be of use in areas like tissue engineering...
  2. ncbi request reprint Silencing of HIV by hairpin-loop-structured DNA oligonucleotide
    Karin Moelling
    Institute of Medical Virology, University of Zurich, Switzerland
    FEBS Lett 580:3545-50. 2006
    ..The ODN against HIV-IIIB was effective preferentially against its homologous PPT and less against the PPT of HIV-BaL differing in two of 24 nucleotides and vice versa. It may be interesting mechanistically as an antiviral drug...
  3. ncbi request reprint Regulation of epithelial wound closure and intercellular adhesion by interaction of AF6 with actin cytoskeleton
    Mihaela Lorger
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    J Cell Sci 119:3385-98. 2006
    ....
  4. ncbi request reprint Anti-tumor activity of mesenchymal stem cells producing IL-12 in a mouse melanoma model
    Lina Elzaouk
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Exp Dermatol 15:865-74. 2006
    ..The presented data demonstrate that these two different strategies can induce a similar therapeutic anti-tumor efficacy in the mouse melanoma tumor model...
  5. ncbi request reprint WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Czeta
    Thorsten Fritzius
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, Zurich CH 8006, Switzerland
    FEBS J 274:1552-66. 2007
    ..VAMP2 is known to regulate docking and fusion of vesicles and to play a role in targeting vesicles to the plasma membrane. The complex may be involved in vesicle cycling in various secretory pathways...
  6. doi request reprint Oligonucleotide-mediated retroviral RNase H activation leads to reduced HIV-1 titer in patient-derived plasma
    Jochen Heinrich
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    AIDS 23:213-21. 2009
    ..The retroviral RNase H is essential for viral replication. This component has not yet been extensively studied for antiviral therapy. It can be activated by an oligodeoxynucleotide (ODN) resulting in self-destruction of the virions...
  7. ncbi request reprint IP-10-encoding plasmid DNA therapy exhibits anti-tumor and anti-metastatic efficiency
    Johanna Keyser
    Institute of Medical Virology, University of Zurich, Gloriastr, Zurich, Switzerland
    Exp Dermatol 13:380-90. 2004
    ..Interestingly, treatment of nude mice lacking T lymphocytes with DNA-encoding IP-10 or IL-12 still resulted in a pronounced reduction of tumor growth or metastasis formation...
  8. pmc Akt- and Foxo1-interacting WD-repeat-FYVE protein promotes adipogenesis
    Thorsten Fritzius
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    EMBO J 27:1399-410. 2008
    ..Thus, ProF modulates Foxo1 phosphorylation by Akt, promoting adipocyte differentiation. Furthermore, ProF might be involved in metabolic disorders such as diabetes...
  9. pmc c-Src-mediated epithelial cell migration and invasion regulated by PDZ binding site
    Martin Baumgartner
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    Mol Cell Biol 28:642-55. 2008
    ..We propose that the C terminus of c-Src via its GENL sequence presents a mechanism that restricts c-Src in epithelia and prevents progression toward an invasive phenotype...
  10. doi request reprint The PDZ protein MPP2 interacts with c-Src in epithelial cells
    Martin Baumgartner
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Exp Cell Res 315:2888-98. 2009
    ..In conclusion we demonstrate here that MPP2 interacts with c-Src in cells to control c-Src activity and morphological function...
  11. ncbi request reprint Intratumoral injection of DNA encoding human interleukin 12 into patients with metastatic melanoma: clinical efficacy
    Lucie Heinzerling
    Department of Dermatology, University of Zurich, CH 8091 Zurich, Switzerland
    Hum Gene Ther 16:35-48. 2005
    ..Serum levels revealed fluctuations. The results show that intratumoral injection of DNA produced some beneficial clinical effect. DNA encoding a cytokine may be useful as a therapeutic or adjuvant against various human cancers...
  12. doi request reprint A short hairpin DNA analogous to miR-125b inhibits C-Raf expression, proliferation, and survival of breast cancer cells
    Marco H Hofmann
    University of Zurich, Zurich, Switzerland
    Mol Cancer Res 7:1635-44. 2009
    ..Both features may be useful to interfere with tumor growth...
  13. doi request reprint Antitumor activity of small double-stranded oligodeoxynucleotides targeting telomerase RNA in malignant melanoma cells
    Faiza Noreen
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Oligonucleotides 19:169-78. 2009
    ..Our study demonstrates the potential utility of these hairpin-loop-structured ODNs as a different group of nucleic acids for telomerase-based antiproliferative strategies...
  14. ncbi request reprint Role of AF6 protein in cell-to-cell spread of Herpes simplex virus 1
    Johanna Keyser
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    FEBS Lett 581:5349-54. 2007
    ..Thus, AF6 protein is important for spreading of HSV-1 in epithelial cells, independently of nectin clustering, possibly by stabilization of the E-cadherin-dependent cell adhesion...
  15. doi request reprint Reduction of gene expression by a hairpin-loop structured oligodeoxynucleotide: alternative to siRNA and antisense
    Terry Kwok
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    Biochim Biophys Acta 1790:1170-8. 2009
    ..ODN A was shown to lead to reduced viral RNA in virions or early during infection...
  16. doi request reprint Inhibition of influenza A virus replication by short double-stranded oligodeoxynucleotides
    Terry Kwok
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Arch Virol 154:109-14. 2009
    ..Thus, dsODNs may be developed as an additional class of nucleic acids for the inhibition of influenza virus replication...
  17. pmc Regulation of c-Src by binding to the PDZ domain of AF-6
    Gerald Radziwill
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    EMBO J 26:2633-44. 2007
    ..c-Src is recruited by AF-6 to cell-cell contact sites, suggesting that c-Src is regulated by a PDZ protein in special cellular locations. We identified a novel type of c-Src regulation by interaction with a PDZ protein...
  18. doi request reprint Retroviral self-inactivation in the mouse vagina induced by short DNA
    Lina Wittmer-Elzaouk
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30 32, CH 8006 Zurich, Switzerland
    Antiviral Res 82:22-8. 2009
    ..Our finding may have some relevance for the design of other viral self-destruction approaches. It may lead to a microbicide for reduction of sexual and mother-to-child transmission...
  19. pmc Short hairpin-loop-structured oligodeoxynucleotides reduce HSV-1 replication
    Alexander Falkenhagen
    University of Zurich, Gloriastrasse 30 32, CH 8006 Zurich, Switzerland
    Virol J 6:43. 2009
    ..The results underline the potential of partially double-stranded hairpin loop-structured ODNs as antiviral agents...
  20. ncbi request reprint Heteromeric complex formation of ASK2 and ASK1 regulates stress-induced signaling
    Elisabeth Ortner
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    Biochem Biophys Res Commun 362:454-9. 2007
    ..We propose that ASK2 regulates stress-induced caspase-3 and PARP cleavage in a dose-dependent manner by heteromeric complex formation with ASK1...
  21. ncbi request reprint A combination of plasmid DNAs encoding murine fetal liver kinase 1 extracellular domain, murine interleukin-12, and murine interferon-gamma inducible protein-10 leads to tumor regression and survival in melanoma-bearing mice
    Kristin Ladell
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8028, Zurich, Switzerland
    J Mol Med (Berl) 81:271-8. 2003
    ..A combination of Flk-1 ECD and DNA encoding murine interleukin-12 or murine interferon-gamma inducible protein-10 improved the effect, leading to tumor regression and long-term survival of the mice...
  22. doi request reprint Vero cells as a model to study the effects of adenoviral gene delivery vectors on the RNAi system in context of viral infection
    Alexey A Matskevich
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    J Innate Immun 1:389-94. 2009
    ..Thus, the Vero cell line could serve as an interesting model for studying the effects of gene delivery vectors on the RNAi system in the context of virus-related disorders...
  23. pmc A WD-FYVE protein binds to the kinases Akt and PKCzeta/lambda
    Thorsten Fritzius
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    Biochem J 399:9-20. 2006
    ..In summary, we describe a new binding partner for kinases, located on vesicular structures in specialized cells, which may play a role for the spatial organization of signalling cascades...
  24. ncbi request reprint c-Src is a PDZ interaction partner and substrate of the E3 ubiquitin ligase Ligand-of-Numb protein X1
    Andreas Weiss
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8006 Zurich, Switzerland
    FEBS Lett 581:5131-6. 2007
    ..Furthermore, we show that c-Src phosphorylates LNX1. Moreover, c-Src itself is ubiquitinated by LNX1, suggesting an interdependent regulation of c-Src and LNX1...
  25. ncbi request reprint Linear closed mini DNA generated by the prokaryotic cleaving-joining enzyme TelN is functional in mammalian cells
    Jochen Heinrich
    Institut fur Medizinische Virologie, Universitat Zurich, Gloriastrasse 30, 8028 Zurich, Switzerland
    J Mol Med (Berl) 80:648-54. 2002
    ..Therefore, the TelN/ telRL system is well suited to generate linear closed mini DNA with high stability and a minimum of foreign nucleotide sequences...
  26. ncbi request reprint RNase H-mediated retrovirus destruction in vivo triggered by oligodeoxynucleotides
    Kathrin Matzen
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8006 Zurich, Switzerland
    Nat Biotechnol 25:669-74. 2007
    ..As it targets events common to the replication cycle of all retroviruses, this approach may be broadly applicable to retroviruses of medical and agricultural importance...
  27. ncbi request reprint Analysis of antitumor activity elicited by vaccination with combinations of interleukin-12 DNA with gp100 DNA or the chemokine CCL21 in vivo
    Lina Elzaouk
    Institute of Medical Virology, University of Zurich, CH 8006 Zurich, Switzerland
    Hum Gene Ther 17:859-70. 2006
    ..These findings open a new route of investigation of IL-12 and gp100 or other tumor-associated antigens in the immunotherapy of a variety of tumors...
  28. ncbi request reprint JM4 is a four-transmembrane protein binding to the CCR5 receptor
    Marc Schweneker
    Institute of Medical Virology, University Zurich, Gloriastr 30, 8028 Zurich, Switzerland
    FEBS Lett 579:1751-8. 2005
    ..In conclusion, we describe a CCR5-interacting protein, which is suggested to function in trafficking and membrane localisation of the receptor, possibly also other receptors or amino acid transporters...
  29. ncbi request reprint Dicer is involved in protection against influenza A virus infection
    Alexey A Matskevich
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30 32, CH 8006 Zurich, Switzerland
    J Gen Virol 88:2627-35. 2007
    ..Thus, RNAi is involved in antiviral response, and Dicer is important for protection against influenza A virus infection...
  30. ncbi request reprint CNK1 is a scaffold protein that regulates Src-mediated Raf-1 activation
    Algirdas Ziogas
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    J Biol Chem 280:24205-11. 2005
    ..Down-regulation of endogenously expressed CNK1 by small inhibitory RNA interferes with Src-dependent activation of ERK. Thus, CNK1 allows cross-talk between Src and Raf-1 and is essential for the full activation of Raf-1...
  31. pmc Short hairpin-looped oligodeoxynucleotides reduce hepatitis C virus replication
    Felix Broecker
    University of Zurich, Zurich, Switzerland
    Virol J 9:134. 2012
    ..Furthermore, standard therapy is associated with severe side-effects. Thus, alternative therapeutic approaches against HCV are needed...
  32. doi request reprint Premature activation of the HIV RNase H drives the virus into suicide: a novel microbicide?
    Felix Broecker
    University of Zurich, CH 8006 Zurich, Switzerland
    AIDS Res Hum Retroviruses 28:1397-403. 2012
    ..Infection was reduced up to 1000-fold or could be completely prevented. The compound is suitable as microbicide or to prevent mother-to-child transmission...
  33. ncbi request reprint RIP2 mediates LPS-induced p38 and IkappaBalpha signaling including IL-12 p40 expression in human monocyte-derived dendritic cells
    Nurguel Usluoglu
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Eur J Immunol 37:2317-25. 2007
    ..Thus, in human DC LPS stimulates a signal cascade that involves the Raf-dependent activation of RIP2 leading to expression of IL-12 p40...
  34. doi request reprint A short hairpin loop-structured oligodeoxynucleotide targeting the virion-associated RNase H of HIV inhibits HIV production in cell culture and in huPBL-SCID mice
    Jochen Heinrich
    University of Zurich, Zurich, Switzerland
    Intervirology 55:242-6. 2012
    ..This mechanism has been described previously in a mouse model using an oncogenic retrovirus and was commented as driving HIV into suicide. The RNase H is one of four retrovirus-specific enzymes and not yet targeted by antiviral drugs...
  35. ncbi request reprint Bcr interferes with beta-catenin-Tcf1 interaction
    Angelika Ress
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    FEBS Lett 580:1227-30. 2006
    ..Thus two independent mechanisms may regulate Tcf/beta-catenin-mediated transcription via Bcr: binding to beta-catenin as we have previously shown and to Tcf1 as shown here...
  36. ncbi request reprint Antibodies from a DNA peptide vaccination decrease the brain amyloid burden in a mouse model of Alzheimer's disease
    Jan G Schiltz
    Institute if Medical Virology, University of Zurich, Gloriastrasse 30, 8028 Zurich, Switzerland
    J Mol Med (Berl) 82:706-14. 2004
    ..The monoclonal antibodies are applicable for passive immunization studies and may lead to a therapy of AD...
  37. ncbi request reprint Interaction partners of the PDZ domain of erbin
    Angelika Ress
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH 8006 Zurich, Switzerland
    Protein Pept Lett 13:877-81. 2006
    ..The interactions were verified by co-immunoprecipitation experiments. These findings demonstrate the promiscuity of the PDZ domain of Erbin...
  38. doi request reprint Stimuli-dependent cleavage of Dicer during apoptosis
    Alexey A Matskevich
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30 32, CH 8006 Zurich, Switzerland
    Biochem J 412:527-34. 2008
    ..Thus the apoptotic machinery may regulate the miRNA pathway by affecting individual proteins, such as Dicer...
  39. ncbi request reprint Short partially double-stranded oligodeoxynucleotide induces reverse transcriptase/RNase H-mediated cleavage of HIV RNA and contributes to abrogation of infectivity of virions
    Alexey A Matskevich
    Institute of Medical Virology, University of Zurich, CH 8006 Zurich, Switzerland
    AIDS Res Hum Retroviruses 22:1220-30. 2006
    ..The pretreatment with ODN A strongly reduced the infectivity of virions in cell culture in the absence of any DNA carriers or detergents...
  40. doi request reprint Targeting the retroviral ribonuclease H by rational drug design
    Karin Moelling
    Institute for Medical Microbiology, University of Zurich, Zurich, Switzerland
    AIDS 26:1983-93. 2012
    ..Some details will be discussed here. Furthermore, a compound is described, which enables the RNase H to kill cell-free HIV particles by driving the virus into suicide - with potential use as a microbicide...
  41. ncbi request reprint The HIV-1 co-receptor CCR5 binds to alpha-catenin, a component of the cellular cytoskeleton
    Marc Schweneker
    Institute of Medical Virology, University of Zurich, 8028 Zurich, Switzerland Institute of Biochemistry, Freie Universitat Berlin, 14195 Berlin, Germany
    Biochem Biophys Res Commun 325:751-7. 2004
    ..Our results suggest that alpha-catenin links CCR5 and CXCR4 to the cytoskeleton and is involved in the organization of these receptors at the membrane, thereby possibly affecting HIV-1 infection...
  42. doi request reprint Analysis of the Intestinal Microbiome of a Recovered Clostridium difficile Patient after Fecal Transplantation
    Felix Broecker
    Institute of Medical Microbiology, University of Zurich, University Hospital Zurich, Zurich, Switzerland
    Digestion 88:243-51. 2013
    ..difficile. The procedure is simple, cheap, caused no side effects, and was stable over 3 years. © 2013 S. Karger AG, Basel. ..
  43. doi request reprint RNase H: specificity, mechanisms of action, and antiviral target
    Karin Moelling
    Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland
    Methods Mol Biol 1087:71-84. 2014
    ..This assay may be used to detect the RNase H, assess the effect of inhibitors, or even activators, of the RNase H, as we have described, as candidates for novel antiretroviral agents...
  44. doi request reprint Photodynamic agents with anti-metastatic activities
    Balayeshwanth R Vummidi
    Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    ACS Chem Biol 8:1737-46. 2013
    ....
  45. pmc Bcr is a negative regulator of the Wnt signalling pathway
    Angelika Ress
    Institute of Medical Virology, University of Zurich, Gloriastrasse 30, 8006 Zurich, Switzerland
    EMBO Rep 6:1095-100. 2005
    ..The inhibitor of the Bcr-Abl tyrosine kinase, STI-571 or Gleevec, a drug against CML, reverses this effect. Our data contribute to the understanding of Bcr as a tumour suppressor in the Wnt signalling pathway, as well as in CML...
  46. ncbi request reprint Tumor regression of human and murine melanoma after intratumoral injection of IL-12-encoding plasmid DNA in mice
    Lucie Heinzerling
    Institute of Medical Virology, University of Zurich, Zurich, Switzerland
    Exp Dermatol 11:232-40. 2002
    ..These results suggest that intratumoral plasmid DNA coding for IL-12 holds some promise as a new therapeutic tool for accessible melanoma lesions and should be tested in clinical trial...
  47. ncbi request reprint Cooperation of Ras- and c-Myc-dependent pathways in regulating the growth and invasiveness of synovial fibroblasts in rheumatoid arthritis
    Thomas Pap
    Center of Internal Medicine, University Hospital Magdeburg, Magdeburg, Germany
    Arthritis Rheum 50:2794-802. 2004
    ..To study the specific contribution of MAP kinase activator c-Raf-1 and one of its downstream transcription factors, c-Myc, to the growth and invasive behavior of rheumatoid arthritis synovial fibroblasts (RASFs)...
  48. ncbi request reprint Characterization of a putative phosphorylation switch: adaptation of SPOT synthesis to analyze PDZ domain regulation mechanisms
    Prisca Boisguerin
    Institut fur Medizinische Immunologie, Charite Universitatsmedizin Berlin, Hessische Strasse 3 4, 10115 Berlin, Germany
    Chembiochem 8:2302-7. 2007
    ..This should ultimately help in the identification of phosphorylation-dependent regulation mechanisms in vivo...