Research Topics
| Oliver MühlemannSummaryAffiliation: University of Bern Country: Switzerland Publications
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Publications
Recognition and elimination of nonsense mRNAOliver Mühlemann
Institute of Cell Biology, University of Berne, Baltzerstrasse 4, CH 3012 Bern, Switzerland
Biochim Biophys Acta 1779:538-49. 2008..Sci. 31 (2006) 639-646.[1]]. In this review, we focus on recent results from different model organisms that indicate an evolutionarily conserved mechanism for PTC identification...
How and where are nonsense mRNAs degraded in mammalian cells?Oliver Mühlemann
Institute of Cell Biology, University of Bern, Bern, Switzerland
RNA Biol 7:28-32. 2010..It therefore remains an open question whether NMD in human cells is restricted to a particular cellular location or whether it can be initiated wherever translation of the NMD substrate takes place...- Recognition of nonsense mRNA: towards a unified modelOliver Mühlemann
Institute of Cell Biology, University of Berne, Baltzerstrasse 4, CH 3012 Berne, Switzerland
Biochem Soc Trans 36:497-501. 2008.... 
Efficient downregulation of immunoglobulin mu mRNA with premature translation-termination codons requires the 5'-half of the VDJ exonMarc Buhler
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
Nucleic Acids Res 32:3304-15. 2004..Moreover, deletion of this NMD-promoting element from the Ig-micro minigene results in loss of strong NMD...- Transcriptional silencing of nonsense codon-containing immunoglobulin minigenesMarc Buhler
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
Mol Cell 18:307-17. 2005..Remarkably, NMTGS is inhibited by overexpression of the putative siRNase 3'hExo, suggesting that siRNA-like molecules are involved in NMTGS... - EJC-independent degradation of nonsense immunoglobulin-mu mRNA depends on 3' UTR lengthMarc Buhler
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
Nat Struct Mol Biol 13:462-4. 2006..As in yeast, this exon junction complex-independent NMD of Ig-mu mRNAs depends on the distance between the termination codon and the poly(A) tail and suggests an evolutionarily conserved mode of PTC recognition... - Intranuclear degradation of nonsense codon-containing mRNAMarc Buhler
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, Switzerland
EMBO Rep 3:646-51. 2002..Our results are fully consistent with recently reported evidence for nuclear translation and suggest that an important biological role for nuclear ribosomes is the early elimination of nonsense mRNA during a pioneer round of translation... - Alternative splicing induced by nonsense mutations in the immunoglobulin mu VDJ exon is independent of truncation of the open reading frameMarc Buhler
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
RNA 11:139-46. 2005.... - Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factorsPamela Nicholson
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
Cell Mol Life Sci 67:677-700. 2010..In recent years, the discovery of additional functions played by several of the NMD factors has further complicated the picture. Therefore, we also review the reported roles of UPF1, SMG1 and SMG6 in other cellular processes... - Processing bodies are not required for mammalian nonsense-mediated mRNA decayLukas Stalder
Institute of Cell Biology, University of Berne, 3012 Berne, Switzerland
RNA 15:1265-73. 2009..Consistent with the recently reported decapping-independent SMG6-mediated endonucleolytic decay of human nonsense mRNAs, our results imply that microscopically detectable P-bodies are not required for mammalian NMD... - Posttranscriptional gene regulation by spatial rearrangement of the 3' untranslated regionAndrea B Eberle
Institute of Cell Biology, University of Berne, Berne, Switzerland
PLoS Biol 6:e92. 2008..Most importantly, our results demonstrate that spatial rearrangements of the 3' untranslated region can modulate the NMD pathway and thereby provide a novel mechanism for posttranscriptional gene regulation... - The meaning of nonsenseLukas Stalder
Institute of Cell Biology, University of Berne, Baltzerstrabetae 4, 3012 Berne, Switzerland
Trends Cell Biol 18:315-21. 2008..Here, we summarize the latest results and discuss an emerging model for NMD and its implications for the regulation of gene expression... - SMG6 promotes endonucleolytic cleavage of nonsense mRNA in human cellsAndrea B Eberle
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
Nat Struct Mol Biol 16:49-55. 2009..Our data lead to a revised mechanistic model for degradation of nonsense mRNA in human cells and suggest that endonucleolytic cleavage is a conserved feature in metazoan NMD... - Analysis of nonsense-mediated mRNA decay in mammalian cellsPamela Nicholson
Department for Chemistry, University of Bern, Bern, Switzerland
Curr Protoc Cell Biol . 2012.... - mRNP quality control goes regulatoryOliver Mühlemann
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH 3012 Bern, Switzerland
Trends Genet 28:70-7. 2012..From this perspective, QC and gene regulation represent two outcomes of the same molecular process... - tRNASec is transcribed by RNA polymerase II in Trypanosoma brucei but not in humansEric Aeby
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH 3012 Bern, Switzerland
Nucleic Acids Res 38:5833-43. 2010..Moreover, intron-encoded tRNAs are present in a number of eukaryotic genomes indicating that Pol-II transcription of tRNAs may not be restricted to trypanosomatids... - Cutting the nonsense: the degradation of PTC-containing mRNAsPamela Nicholson
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH 3012 Bern, Switzerland
Biochem Soc Trans 38:1615-20. 2010.... - Equal transcription rates of productively and nonproductively rearranged immunoglobulin mu heavy chain alleles in a pro-B cell lineAndrea B Eberle
Institute of Cell Biology, University of Bern, 3012 Bern, Switzerland
RNA 15:1021-8. 2009..Altogether, we found no evidence for transcriptional silencing of the PTC+ allele in this pro-B cell line; hence, the efficient down-regulation of the PTC+ Ig-mu mRNA results entirely from NMD... - Autoregulation of the nonsense-mediated mRNA decay pathway in human cellsHasmik Yepiskoposyan
Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland
RNA 17:2108-18. 2011..Using reporter gene assays, we demonstrated that the long 3' UTRs of UPF1, SMG5, and SMG7 mRNAs are the main NMD-inducing features of these mRNAs, suggesting that long 3' UTRs might be a frequent trigger of NMD... - Transcriptional silencing of nonsense codon-containing immunoglobulin micro genes requires translation of its mRNALukas Stalder
Institute of Cell Biology, University of Berne, Baltzerstrasse 4, CH 3012 Bern, Switzerland
J Biol Chem 282:16079-85. 2007..Collectively, our data indicate that NMTGS and NMD are linked, relying on the same mechanism for PTC recognition, and that the NMTGS pathway branches from the NMD pathway at a step after Upf1 function... - A GFP-based reporter system to monitor nonsense-mediated mRNA decayAlexandra Paillusson
Institute of Cell Biology, University of Bern CH-3012 Bern, Switzerland
Nucleic Acids Res 33:e54. 2005..With these properties, our GFP-based NMD reporter system could be used for large-scale screenings to identify NMD-inhibiting drugs or NMD-deficient mutant cells... - Nonsense-associated alternative splicing of T-cell receptor beta genes: no evidence for frame dependenceFabio Mohn
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
RNA 11:147-56. 2005..Our study thus contradicts the previously reported PTC specificity of TCR-beta NAS and points out the need for systematically testing the PTC specificity in other cases where NAS has been observed...