W Keller

Summary

Affiliation: University of Basel
Country: Switzerland

Publications

  1. ncbi request reprint Editing of messenger RNA precursors and of tRNAs by adenosine to inosine conversion
    W Keller
    Biozentrum, University of Basel, Switzerland
    FEBS Lett 452:71-6. 1999
  2. ncbi request reprint A comparison of mammalian and yeast pre-mRNA 3'-end processing
    W Keller
    Department of Cell Biology, Biozentrum University of Basel, Klingelbergstrasse 70, CH 4056, Basel, Switzerland
    Curr Opin Cell Biol 9:329-36. 1997
  3. ncbi request reprint Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I
    A Jenny
    Department of Cell Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Science 274:1514-7. 1996
  4. pmc Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors
    H de Vries
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Germany
    EMBO J 19:5895-904. 2000
  5. pmc A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor
    P J Preker
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    EMBO J 16:4727-37. 1997
  6. pmc Distinct roles of two Yth1p domains in 3'-end cleavage and polyadenylation of yeast pre-mRNAs
    S M Barabino
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 19:3778-87. 2000
  7. pmc The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex
    M Ohnacker
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 19:37-47. 2000
  8. pmc Control of cleavage site selection during mRNA 3' end formation by a yeast hnRNP
    L Minvielle-Sebastia
    Department of Cell Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    EMBO J 17:7454-68. 1998
  9. pmc Two forms of human double-stranded RNA-specific editase 1 (hRED1) generated by the insertion of an Alu cassette
    A Gerber
    Department of Cell Biology, Biozentrum of the University of Basel, Switzerland
    RNA 3:453-63. 1997
  10. ncbi request reprint RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor
    L Minvielle-Sebastia
    Department of Cell Biology, University of Basel, Switzerland
    Science 266:1702-5. 1994

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Editing of messenger RNA precursors and of tRNAs by adenosine to inosine conversion
    W Keller
    Biozentrum, University of Basel, Switzerland
    FEBS Lett 452:71-6. 1999
    ..Tad1p could be the ancestor of ADAR1 and ADAR2. The deaminase domains of ADAR1, ADAR2 and Tad1p are very similar and resemble the active site domains of cytosine/cytidine deaminases...
  2. ncbi request reprint A comparison of mammalian and yeast pre-mRNA 3'-end processing
    W Keller
    Department of Cell Biology, Biozentrum University of Basel, Klingelbergstrasse 70, CH 4056, Basel, Switzerland
    Curr Opin Cell Biol 9:329-36. 1997
    ..Most of the mammalian 3'-end-processing factors are similar in their amino acid sequence to the yeast factors, indicating that they have a common evolutionary history...
  3. ncbi request reprint Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I
    A Jenny
    Department of Cell Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Science 274:1514-7. 1996
    ..This finding was unexpected because in contrast to CPSF, PFI is only required for the polyadenylation reaction. These results contribute to the understanding of how 3'-end processing factors may have evolved...
  4. pmc Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors
    H de Vries
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Germany
    EMBO J 19:5895-904. 2000
    ..hClp1 interacts with CF I(m) and the cleavage and polyadenylation specificity factor CPSF, suggesting that it bridges these two 3' end processing factors within the cleavage complex...
  5. pmc A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor
    P J Preker
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    EMBO J 16:4727-37. 1997
    ..PF I also appears to be functionally related to CPSF, as it polyadenylates a substrate RNA more efficiently than Pap1p alone. Possibly, the observed interaction of the complex with RNA tethers Pap1p to its substrate...
  6. pmc Distinct roles of two Yth1p domains in 3'-end cleavage and polyadenylation of yeast pre-mRNAs
    S M Barabino
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 19:3778-87. 2000
    ..Finally, we find that Yth1p binds to CYC1 pre-mRNA in the vicinity of the cleavage site. Our results indicate that Yth1p is important for the integrity of CPF and participates in the recognition of the cleavage site...
  7. pmc The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex
    M Ohnacker
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 19:37-47. 2000
    ..These results show that Pfs2p plays an essential role in 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex...
  8. pmc Control of cleavage site selection during mRNA 3' end formation by a yeast hnRNP
    L Minvielle-Sebastia
    Department of Cell Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    EMBO J 17:7454-68. 1998
    ..Addition of Nab4p/Hrp1p prevents these alternative cleavages in a concentration-dependent manner, suggesting an essential and conserved role for some hnRNPs in pre-mRNA cleavage site selection...
  9. pmc Two forms of human double-stranded RNA-specific editase 1 (hRED1) generated by the insertion of an Alu cassette
    A Gerber
    Department of Cell Biology, Biozentrum of the University of Basel, Switzerland
    RNA 3:453-63. 1997
    ..In vitro editing assays with purified recombinant hRED1 containing or lacking the Alu-J cassette revealed that both forms of the protein have the same substrate specificity, but differ in their catalytic activity...
  10. ncbi request reprint RNA14 and RNA15 proteins as components of a yeast pre-mRNA 3'-end processing factor
    L Minvielle-Sebastia
    Department of Cell Biology, University of Basel, Switzerland
    Science 266:1702-5. 1994
    ..Biochemical complementation experiments and reconstitution of both activities with partially purified cleavage factor I (CF I) validated the genetic prediction...
  11. pmc Crystal structure of mammalian poly(A) polymerase in complex with an analog of ATP
    G Martin
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    EMBO J 19:4193-203. 2000
    ..Furthermore, conserved, catalytically important residues contact the nucleotide. These contacts, taken together with metal coordination of the adenine base, provide a structural basis for ATP selection by poly(A) polymerase...
  12. ncbi request reprint The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase
    P J Preker
    Department of Cell Biology, University of Basel, Switzerland
    Cell 81:379-89. 1995
    ..We propose a model in which PF I tethers PAP1 to CF I, thereby conferring specificity to poly(A) polymerase for pre-mRNA substrates...
  13. pmc Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor
    A Jenny
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    Nucleic Acids Res 23:2629-35. 1995
    ..The sequence contains a possible nuclear localisation signal but none of the known RNA binding motifs. It does, however, show sequence similarities to a UV-damaged DNA binding protein (UVdDb)...
  14. ncbi request reprint Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits
    U Rüegsegger
    Department of Cell Biology, Universitat Basel, Switzerland
    Mol Cell 1:243-53. 1998
    ..Analysis of the kinetics of the cleavage reaction indicates that interaction of CF Im with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors...
  15. ncbi request reprint The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins
    S M Barabino
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    Genes Dev 11:1703-16. 1997
    ..Efficient polyadenylation activity can be restored by the addition of purified polyadenylation factor I (PF I). We demonstrate that Yth1p is a component of PF I that interacts in vivo and in vitro with Fip1p, a known PF I subunit...
  16. pmc The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue
    M Ohnacker
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    Nucleic Acids Res 24:2585-91. 1996
    ..These results indicate a high degree of conservation of the polyadenylation machinery among the evolutionary diverged budding and fission yeasts...
  17. pmc Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit
    A Jenny
    Department of Cell Biology, University of Basel, Switzerland
    Mol Cell Biol 14:8183-90. 1994
    ..Immunofluorescent detection of CPSF in HeLa cells localized it in the nucleoplasm, excluding cytoplasm and nucleolar structures...
  18. pmc Recognition of polyadenylation sites in yeast pre-mRNAs by cleavage and polyadenylation factor
    B Dichtl
    Department of Cell Biology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland
    EMBO J 20:3197-209. 2001
    ..A model for yeast pre-mRNA cleavage-site recognition outlines an unanticipated high conservation of yeast and mammalian 3'-end processing mechanisms...
  19. ncbi request reprint Purification of human double-stranded RNA-specific editase 1 (hRED1) involved in editing of brain glutamate receptor B pre-mRNA
    M A O'Connell
    Department of Cell Biology, Biozentrum of the University of Basel, Switzerland
    J Biol Chem 272:473-8. 1997
    ..This enzyme is distinct from double-stranded RNA-specific adenosine deaminase which we and others have previously purified and cloned...
  20. ncbi request reprint Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors
    U Rüegsegger
    Department of Cell Biology, Biozentrum, Universitat Basel, Switzerland
    J Biol Chem 271:6107-13. 1996
    ..Furthermore, the CstF-CPSF-RNA as well as the CstF-CPSF-PAP-RNA complex are supershifted and stabilized upon the addition of CF Im...
  21. pmc Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases
    G Martin
    Department of Cell Biology, University of Basel, Basel, Switzerland
    EMBO J 15:2593-603. 1996
    ..This homology extends as far as cca: tRNA nucleotidyltransferase and streptomycin adenylyltransferase, an antibiotic resistance factor...
  22. pmc The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation
    L Minvielle-Sebastia
    Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056, Basel, Switzerland
    Proc Natl Acad Sci U S A 94:7897-902. 1997
    ..Our data support the notion that Pab1p is involved in the length control of the poly(A) tails of yeast mRNAs and define a new essential function for Pab1p in the formation of mature mRNAs...
  23. ncbi request reprint The HAT helix, a repetitive motif implicated in RNA processing
    P J Preker
    Department of Cell Biology, Biozentrum, University of Basel, Switzerland
    Trends Biochem Sci 23:15-6. 1998
  24. pmc Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2
    A Gerber
    Department of Cell Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    EMBO J 17:4780-9. 1998
    ..These findings suggest an evolutionary link between pre-mRNA editing and tRNA modification...
  25. ncbi request reprint An adenosine deaminase that generates inosine at the wobble position of tRNAs
    A P Gerber
    Department of Cell Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland
    Science 286:1146-9. 1999
    ..These results provide an evolutionary link between the CDA superfamily and RNA-dependent adenosine deaminases (ADARs/ADATs)...
  26. ncbi request reprint 3' cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP
    G Christofori
    Department of Cell Biology, Biocenter of the University of Basel, Switzerland
    Cell 54:875-89. 1988
    ..Further purification of CPF by buoyant density centrifugation, ion exchange, and affinity column chromatography or by gel filtration demonstrates that CPF activity resides in a ribonucleoprotein and copurifies with U11 snRNP...
  27. pmc Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase
    M A O'Connell
    Department of Cell Biology, University of Basel, Switzerland
    Mol Cell Biol 15:1389-97. 1995
    ..Staining of HeLa cells confirms the nuclear localization of the dsRNA-specific adenosine deaminase. In situ hybridization in rat brain slices indicates a widespread distribution of the enzyme in the brain...
  28. ncbi request reprint RNA editing by base deamination: more enzymes, more targets, new mysteries
    A P Gerber
    Dept of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
    Trends Biochem Sci 26:376-84. 2001
    ..This superfamily might have evolved from an ancient cytidine deaminase. This article reviews the reactions catalysed by these enzymes and discusses their evolutionary relationships...
  29. pmc The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import
    B Fahrenkrog
    M E Müller Institute for Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 11:3885-96. 2000
    ..Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export...
  30. ncbi request reprint mRNA polyadenylation and its coupling to other RNA processing reactions and to transcription
    L Minvielle-Sebastia
    Department of Cell Biology, Biozentrum University of Basel, Klingelbergstrasse 70, CH 4056, Basel, Switzerland
    Curr Opin Cell Biol 11:352-7. 1999
    ..These links are important for the quality control of the mRNA during synthesis...
  31. pmc Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA
    W Keller
    Department of Cell Biology, University of Basel, Switzerland
    EMBO J 10:4241-9. 1991
    ..The entire hexamer signal is involved in binding of CPF since modification of any of its bases interferes with complex formation...
  32. ncbi request reprint Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae
    J Lingner
    Department of Cell Biology, University of Basel, Switzerland
    Nature 354:496-8. 1991
    ..Poly(A) polymerase purified from recombinant Escherichia coli has the same physical and biochemical properties as the yeast enzyme. The yeast poly(A) polymerase shares features of sequence with its mammalian homologue...
  33. pmc The properties of a tRNA-specific adenosine deaminase from Drosophila melanogaster support an evolutionary link between pre-mRNA editing and tRNA modification
    L P Keegan
    MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, United Kingdom
    Mol Cell Biol 20:825-33. 2000
    ..dAdat1 transcripts are maternally supplied in the egg. Zygotic expression is widespread initially and later concentrates in the central nervous system...
  34. ncbi request reprint Influenza virus NS1 protein interacts with the cellular 30 kDa subunit of CPSF and inhibits 3'end formation of cellular pre-mRNAs
    M E Nemeroff
    Department of Molecular Biology and Biochemistry Rutgers University Piscataway, New Jersey 08854, USA
    Mol Cell 1:991-1000. 1998
    ..Via this novel regulation of pre-mRNA 3' end processing, the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs...
  35. pmc Assembly of a processive messenger RNA polyadenylation complex
    S Bienroth
    Abteilung Zellbiologie, Universitat Basel, Switzerland
    EMBO J 12:585-94. 1993
    ..Only the complex formed from all three proteins is competent for the processive synthesis of a full-length poly(A) tail...
  36. pmc Isolation and expression of cDNA clones encoding mammalian poly(A) polymerase
    E Wahle
    Universitat Basel, Abteilung Zellbiologie, Switzerland
    EMBO J 10:4251-7. 1991
    ..3 kb. The sequence of the N-terminal half of bovine poly(A) polymerase is 47% identical with the amino acid sequence of the corresponding part of yeast poly(A) polymerase. Homologies to other proteins are of uncertain significance...
  37. ncbi request reprint Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity
    H C Fuchs
    Center of Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria
    Clin Exp Allergy 36:359-68. 2006
    ..Pru av 2, identified as a thaumatin-like protein (TLP) from sweet cherry, was recognized by the majority of cherry-allergic patients in immunoblotting...
  38. ncbi request reprint Characterization of a hypoallergenic recombinant Bet v 1 variant as a candidate for allergen-specific immunotherapy
    H Kahlert
    Allergopharma Joachim Ganzer KG, Reinbek, Germany
    Int Arch Allergy Immunol 145:193-206. 2008
    ..Recombinant allergens and especially their hypoallergenic variants are promising candidates for a more effective and safer specific immunotherapy...