Research Topics
Genomes and Genes | P FrancoisSummaryAffiliation: University Hospital Country: Switzerland Publications
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Publications
Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort studyStephan Harbarth
Infection Control Program, Geneva University Hospitals, Geneva, Switzerland
Crit Care 10:R25. 2006....
Inhibition by heparin and derivatized dextrans of Staphylococcus epidermidis adhesion to in vitro fibronectin-coated or explanted polymer surfacesP Francois
Department of Medicine, University Hospital, Geneva, Switzerland
J Biomater Sci Polym Ed 10:1207-21. 1999..epidermidis attachment to implanted biomaterials. Furthermore, S. epidermidis adhesion to fibronectin-coated or implanted biomaterials can be efficiently blocked in vitro by CMDBS...- Lack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidisPatrice Francois
Division of Infectious Diseases, University Hospitals of Geneva, 1211 Geneva 14, Switzerland
FEMS Immunol Med Microbiol 35:135-40. 2003..aureus or S. epidermidis were equivalent to those of their respective ica-negative mutants, in the locally infected fluids and on tissue-cage-inserted plastic coverslips... - Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assayPatrice Francois
Genomic Research Laboratory, Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
J Clin Microbiol 41:254-60. 2003..The complete protocol may provide results in less than 6 h (while standard procedure needs 2 to 3 days), thus allowing prompt and cost-effective implementation of contact precautions... - Introduction of the mec element (methicillin resistance) into Staphylococcus aureus alters in vitro functional activities of fibrinogen and fibronectin adhesinsP E Vaudaux
Division of Infectious Diseases, University Hospital, Geneva, Switzerland
Antimicrob Agents Chemother 42:564-70. 1998..aureus impairs the in vitro functional activities of fibrinogen or fibronectin adhesins without altering their production. This effect is unrelated to the activity of the mecA gene... - Modulation of fibronectin adhesins and other virulence factors in a teicoplanin-resistant derivative of methicillin-resistant Staphylococcus aureusAdriana Renzoni
Division of Infectious Diseases, University Hospitals of Geneva, CH-1211 Geneva 14, Switzerland
Antimicrob Agents Chemother 48:2958-65. 2004..These molecular and phenotypic changes may have a profound impact on the bacterial adhesion and colonization properties of such multiresistant organisms... - Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureusPatrice Francois
Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
J Clin Microbiol 45:2011-3. 2007..Our results suggest that users of orfX-staphylococcal cassette chromosome mec-based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones... - Influence of surface treatments developed for oral implants on the physical and biological properties of titanium. (II) Adsorption isotherms and biological activity of immobilized fibronectinP Francois
Division of Infectious Diseases, University Hospital, Geneva, Switzerland
Clin Oral Implants Res 8:217-25. 1997..Despite adsorbing different amounts of fibronectin, both rough and smooth titanium surfaces promote normal expression of 2 major functional domains of this protein... - Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assayPatrice Francois
Genomic Research Laboratory, University of Geneva Hospitals, Service of Infectious Diseases Genomic Research Laboratory, CH 1211 Geneva 14, Switzerland
J Clin Microbiol 44:1892-5. 2006..aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information... 
Rapid and high-throughput genotyping of Staphylococcus epidermidis isolates by automated multilocus variable-number of tandem repeats: a tool for real-time epidemiologyPatrice Francois
University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
J Microbiol Methods 72:296-305. 2008..This type of rapid and high-throughput methodology opens the possibility to rapidly assess long-term nosocomial transmission or to characterize infecting strains in the general procedure of routine laboratories, in real-time...- Reassessing the role of Staphylococcus aureus clumping factor and fibronectin-binding protein by expression in Lactococcus lactisY A Que
Division of Infectious Diseases, Department of Internal Medicine, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
Infect Immun 69:6296-302. 2001..Taken together, the present observations suggest that if antiadhesin therapy were to be developed, at least both of the clfA and fnbA products should be blocked for the therapy to be effective... - Molecular diagnosis of bloodstream infections: planning to (physically) reach the bedsideN Leggieri
Bacteriology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, rue Gabrielle Perret Gentil, Geneva, Switzerland
Curr Opin Infect Dis 23:311-9. 2010.... - Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolatesPatrice Francois
Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
J Clin Microbiol 43:3346-55. 2005..All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time... - A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elementsPatrice Francois
Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
J Clin Microbiol 42:3309-12. 2004..This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA... - Induction of fibronectin adhesins in quinolone-resistant Staphylococcus aureus by subinhibitory levels of ciprofloxacin or by sigma B transcription factor activity is mediated by two separate pathwaysDongmei Li
Service of Infectious Diseases, University Hospitals of Geneva, Geneva, Switzerland
Antimicrob Agents Chemother 49:916-24. 2005..aureus occurs via independent pathways whose concerted actions may significantly promote bacterial adhesion and colonization... - Risk factors for treatment failure in orthopedic device-related methicillin-resistant Staphylococcus aureus infectionT Ferry
Infectious Diseases Unit, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland
Eur J Clin Microbiol Infect Dis 29:171-80. 2010..2-16.3; P = 0.025) were independent predictors of treatment failure after debridement. Therapy using a combination of antimicrobials should be considered for patients with MRSA ODRI, especially when implant removal is not feasible... - Surveillance of extended-spectrum-beta-lactamase-producing Enterobacteriaceae in a Swiss Tertiary Care HospitalC Fankhauser
Infection Control Programme, University of Geneva Hospitals, Geneva, Switzerland
Swiss Med Wkly 139:747-51. 2009.... - Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss childrenC Mégevand
Department of Paediatrics, University of Geneva Hospitals, Geneva, Switzerland
Clin Microbiol Infect 16:1414-20. 2010..Multilocus sequence typing confirmed this observation of diversity and identified ST45 as a frequent colonizer. This broad diversity in MSSA carriage strains suggests a limited selection pressure in our geographical area... - In vivo emergence of subpopulations expressing teicoplanin or vancomycin resistance phenotypes in a glycopeptide-susceptible, methicillin-resistant strain of Staphylococcus aureusP Vaudaux
Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
J Antimicrob Chemother 47:163-70. 2001..aureus subpopulations exhibiting decreased glycopeptide susceptibility and growing in the presence of otherwise inhibitory concentrations of these antimicrobial agents... - Evaluation of a high-density oligonucleotide array for characterization of grlA, grlB, gyrA and gyrB mutations in fluoroquinolone resistant Staphylococcus aureus isolatesSabine Couzinet
Division of Infectious Diseases, University Hospitals of Geneva, CH 1211, 24 Rue Micheli du Crest, 1211 Geneva 14, Switzerland
J Microbiol Methods 60:275-9. 2005..For the two most important genes, gyrA and grlA, correlation with sequencing reached 95.1%. If all genes were considered, correlation was 88.8%... - Comparative efficacy of daptomycin and vancomycin in the therapy of experimental foreign body infection due to Staphylococcus aureusPierre Vaudaux
Division of Infectious Diseases, Geneva University Hospital, CH 1211 Geneva 14, Switzerland
J Antimicrob Chemother 52:89-95. 2003..aureus. Drug dosage should be adapted to obtain inflammatory fluid levels of daptomycin minimizing emergence of resistant subpopulations... - A recA-LexA-dependent pathway mediates ciprofloxacin-induced fibronectin binding in Staphylococcus aureusCarmelo Bisognano
Division of Infectious Diseases, University Hospital, CH-1211 Geneva 14, Switzerland
J Biol Chem 279:9064-71. 2004..We conclude that activation of recA and derepression of lexA-regulated genes by CPX may represent a response to drug-induced damage that results in a novel induction of a virulence factor leading to increased bacterial tissue adherence... - Use of oligoarrays for characterization of community-onset methicillin-resistant Staphylococcus aureusThibaud Koessler
Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland
J Clin Microbiol 44:1040-8. 2006.... - Increased expression of clumping factor and fibronectin-binding proteins by hemB mutants of Staphylococcus aureus expressing small colony variant phenotypesPierre Vaudaux
Division of Infectious Diseases, University Hospital, CH 1211 Geneva 14, Switzerland
Infect Immun 70:5428-37. 2002..Both agr-positive and agr-lacking hemB mutants were also more efficiently internalized by human embryonic kidney cells than were their isogenic controls, presumably because of increased surface display of their fibronectin adhesins... - Comparison of levofloxacin, alatrofloxacin, and vancomycin for prophylaxis and treatment of experimental foreign-body-associated infection by methicillin-resistant Staphylococcus aureusPierre Vaudaux
Division of Infectious Diseases, Geneva University Hospital, CH 1211 Geneva 14, Switzerland
Antimicrob Agents Chemother 46:1503-9. 2002..The mechanisms explaining the inferior activity of alatrofloxacin compared to the activity of levofloxacin against chronic foreign-body-associated infections by MRSA are unknown... - Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species levelAbdessalam Cherkaoui
Clinical Microbiology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
J Clin Microbiol 48:1169-75. 2010..Our data suggest that implementation of MS as a first test strategy for one-step species identification would improve timeliness and reduce isolate identification costs in clinical bacteriology laboratories now... - Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-2005Patrice Francois
University of Geneva Hospitals, Geneva, Switzerland
Emerg Infect Dis 14:304-7. 2008..We demonstrated strain diversity, including clones rarely found in Europe. Local epidemiology of community-associated MRSA is diverse and is evolving by importation and transmission of new strains... - Proteomic approach to investigate MRSAPatrice Francois
Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, Geneva, Switzerland
Methods Mol Biol 391:179-99. 2007..aureus on the organism level. Coupled with methods analyzing the whole bacterial transcriptome, this approach might contribute to the development of rapid diagnostic tests and to the identification of new drug targets... - Genome content determination in methicillin-resistant Staphylococcus aureusPatrice Francois
Service of Infectious Diseases, Genomic Research Laboratory, Department of Internal Medicine, Geneva 14, Switzerland
Future Microbiol 2:187-98. 2007..In addition, sequence information is mandatory for the development of molecular tests allowing the rapid identification, genotyping and characterization of clinical isolates... - Development and evaluation of a rapid strategy to determine enterotoxin gene content in Staphylococcus aureusAdrien Fischer
Genomic Research Laboratory, Service of Infectious Diseases, University Hospital of Geneva, University of Geneva, CH 1 211 Geneva 14, Switzerland
J Microbiol Methods 77:184-90. 2009..A combined genotyping method that includes rapid enterotoxin content determination appears informative for various epidemiological survey purposes... - High-density DNA probe arrays for identification of staphylococci to the species levelSabine Couzinet
Genomic Research Laboratory, University Hospitals of Geneva Rue Micheli-du-Crest 24, 1211 Geneva 14, Switzerland
J Microbiol Methods 61:201-8. 2005..The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies... - New approaches for functional genomic studies in staphylococciMarie Beaume
Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals HUG, CH 1211 Geneva 14, Switzerland
Int J Med Microbiol 300:88-97. 2010..Here we review the principles, advantages and disadvantages of recent technical developments in the field of transcriptomics and their potential impact on S. aureus research... - Cartography of methicillin-resistant S. aureus transcripts: detection, orientation and temporal expression during growth phase and stress conditionsMarie Beaume
Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals, Geneva, Switzerland
PLoS ONE 5:e10725. 2010..Most studies evaluating gene content have so far neglected to analyze the so-called intergenic regions as well as potential antisense RNA molecules... - Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogensPatrice Francois
Genomic Research Laboratory, Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
J Microbiol Methods 55:755-62. 2003..Our study reveals that nanoparticle-labeled bacterial RNA generates reproducible resonance light scattering signals that are at least 50 times more intense than state-of-the-art confocal-based fluorescence signals... - Comparison of amplification methods for transcriptomic analyses of low abundance prokaryotic RNA sourcesPatrice Francois
Genomic Research Laboratory, Service of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
J Microbiol Methods 68:385-91. 2007.... - Rapid bacterial identification using evanescent-waveguide oligonucleotide microarray classificationPatrice Francois
University Hospitals of Geneva, Genomic Research Laboratory, Service of Infectious Diseases, Switzerland
J Microbiol Methods 65:390-403. 2006..In conclusion, when coupled to optimal signal detection strategy, microarrays provide bacterial identification within a few hours post-sampling, allowing targeted antimicrobial prescription... - Novel microarray design strategy to study complex bacterial communitiesAntoine Huyghe
Genomic Research Laboratory, Infectious Diseases Service, University of Geneva Hospitals, Micheli du Crest 24, 1211 Geneva 14, Geneva, Switzerland
Appl Environ Microbiol 74:1876-85. 2008..Our results show that this hierarchical approach can reveal minor changes (<or=1%) in gingival flora content when samples collected in individuals from similar geographical origins are compared... - Ceftazidime-resistant Klebsiella oxytoca producing an OXY-2-type variant from SwitzerlandJosé Manuel Rodríguez-Martínez
Int J Antimicrob Agents 32:278-9. 2008 - In vivo detection of clonally derived methicillin-resistant/methicillin-susceptible Staphylococcus aureus strains is not a rare eventPatrice Francois
J Clin Microbiol 46:1890-1. 2008 - Nonredundant mass spectrometry: a strategy to integrate mass spectrometry acquisition and analysisAlexander Scherl
Biomedical Proteomics Research Group, Central Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland
Proteomics 4:917-27. 2004..It was also found that this new workflow significantly increased sequence coverage by identifying unexpected peptides, which can result from post-translational modifications... - Evidence of an intracellular reservoir in the nasal mucosa of patients with recurrent Staphylococcus aureus rhinosinusitisSophie Clement
Department of Pathology and Immunology, University of Geneva, Switzerland
J Infect Dis 192:1023-8. 2005..Intracellular residence may provide a sanctuary for pathogenic bacteria by protecting them from host defense mechanisms and antibiotic treatment during acute, recurrent S. aureus rhinosinusitis... - Compliance with guidelines on antibiotic prophylaxis in total hip replacement surgery: results of a retrospective study of 416 patients in a teaching hospitalPierrick Bedouch
, Centre Hospitalier Universitaire de Grenoble, France
Infect Control Hosp Epidemiol 25:302-7. 2004..56; CI95 1.02-2.38). CONCLUSION: The overall compliance rate should be improved by disseminating the guidelines and the results of this study following audit and feedback... - Inactivation of a subpopulation of human neutrophils by exposure to ultrahigh-molecular-weight polyethylene wear debrisLouis Bernard
Service of Orthopaedic Surgery, Geneva University Hospital, Switzerland
FEMS Immunol Med Microbiol 49:425-32. 2007..In conclusion, engulfment of UHMWPE wear particles led to inactivation of S. aureus uptake in nearly half of the neutrophil population, which may potentially impair host clearance mechanisms against pyogenic infections... - A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cellsChristian Garzoni
Service of Infectious Diseases, University Hospital of Geneva, Department of Internal Medicine, Geneva 14, Switzerland
BMC Genomics 8:171. 2007..Mechanisms contributing to internalization have been partly elucidated, but bacterial processes triggered intracellularly are largely unknown...