P Francois

Summary

Affiliation: University Hospital
Country: Switzerland

Publications

  1. pmc Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study
    Stephan Harbarth
    Infection Control Program, Geneva University Hospitals, Geneva, Switzerland
    Crit Care 10:R25. 2006
  2. pmc Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay
    Patrice Francois
    Genomic Research Laboratory, Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 41:254-60. 2003
  3. ncbi request reprint Inhibition by heparin and derivatized dextrans of Staphylococcus epidermidis adhesion to in vitro fibronectin-coated or explanted polymer surfaces
    P Francois
    Department of Medicine, University Hospital, Geneva, Switzerland
    J Biomater Sci Polym Ed 10:1207-21. 1999
  4. ncbi request reprint Lack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidis
    Patrice Francois
    Division of Infectious Diseases, University Hospitals of Geneva, 1211 Geneva 14, Switzerland
    FEMS Immunol Med Microbiol 35:135-40. 2003
  5. pmc Introduction of the mec element (methicillin resistance) into Staphylococcus aureus alters in vitro functional activities of fibrinogen and fibronectin adhesins
    P E Vaudaux
    Division of Infectious Diseases, University Hospital, Geneva, Switzerland
    Antimicrob Agents Chemother 42:564-70. 1998
  6. pmc Modulation of fibronectin adhesins and other virulence factors in a teicoplanin-resistant derivative of methicillin-resistant Staphylococcus aureus
    Adriana Renzoni
    Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    Antimicrob Agents Chemother 48:2958-65. 2004
  7. pmc Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus
    Patrice Francois
    Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 45:2011-3. 2007
  8. ncbi request reprint Influence of surface treatments developed for oral implants on the physical and biological properties of titanium. (II) Adsorption isotherms and biological activity of immobilized fibronectin
    P Francois
    Division of Infectious Diseases, University Hospital, Geneva, Switzerland
    Clin Oral Implants Res 8:217-25. 1997
  9. pmc Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, Service of Infectious Diseases Genomic Research Laboratory, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 44:1892-5. 2006
  10. doi request reprint Rapid and high-throughput genotyping of Staphylococcus epidermidis isolates by automated multilocus variable-number of tandem repeats: a tool for real-time epidemiology
    Patrice Francois
    University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 72:296-305. 2008

Collaborators

Detail Information

Publications45

  1. pmc Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study
    Stephan Harbarth
    Infection Control Program, Geneva University Hospitals, Geneva, Switzerland
    Crit Care 10:R25. 2006
    ....
  2. pmc Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay
    Patrice Francois
    Genomic Research Laboratory, Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 41:254-60. 2003
    ..The complete protocol may provide results in less than 6 h (while standard procedure needs 2 to 3 days), thus allowing prompt and cost-effective implementation of contact precautions...
  3. ncbi request reprint Inhibition by heparin and derivatized dextrans of Staphylococcus epidermidis adhesion to in vitro fibronectin-coated or explanted polymer surfaces
    P Francois
    Department of Medicine, University Hospital, Geneva, Switzerland
    J Biomater Sci Polym Ed 10:1207-21. 1999
    ..epidermidis attachment to implanted biomaterials. Furthermore, S. epidermidis adhesion to fibronectin-coated or implanted biomaterials can be efficiently blocked in vitro by CMDBS...
  4. ncbi request reprint Lack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidis
    Patrice Francois
    Division of Infectious Diseases, University Hospitals of Geneva, 1211 Geneva 14, Switzerland
    FEMS Immunol Med Microbiol 35:135-40. 2003
    ..aureus or S. epidermidis were equivalent to those of their respective ica-negative mutants, in the locally infected fluids and on tissue-cage-inserted plastic coverslips...
  5. pmc Introduction of the mec element (methicillin resistance) into Staphylococcus aureus alters in vitro functional activities of fibrinogen and fibronectin adhesins
    P E Vaudaux
    Division of Infectious Diseases, University Hospital, Geneva, Switzerland
    Antimicrob Agents Chemother 42:564-70. 1998
    ..aureus impairs the in vitro functional activities of fibrinogen or fibronectin adhesins without altering their production. This effect is unrelated to the activity of the mecA gene...
  6. pmc Modulation of fibronectin adhesins and other virulence factors in a teicoplanin-resistant derivative of methicillin-resistant Staphylococcus aureus
    Adriana Renzoni
    Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    Antimicrob Agents Chemother 48:2958-65. 2004
    ..These molecular and phenotypic changes may have a profound impact on the bacterial adhesion and colonization properties of such multiresistant organisms...
  7. pmc Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus
    Patrice Francois
    Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 45:2011-3. 2007
    ..Our results suggest that users of orfX-staphylococcal cassette chromosome mec-based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones...
  8. ncbi request reprint Influence of surface treatments developed for oral implants on the physical and biological properties of titanium. (II) Adsorption isotherms and biological activity of immobilized fibronectin
    P Francois
    Division of Infectious Diseases, University Hospital, Geneva, Switzerland
    Clin Oral Implants Res 8:217-25. 1997
    ..Despite adsorbing different amounts of fibronectin, both rough and smooth titanium surfaces promote normal expression of 2 major functional domains of this protein...
  9. pmc Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, Service of Infectious Diseases Genomic Research Laboratory, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 44:1892-5. 2006
    ..aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information...
  10. doi request reprint Rapid and high-throughput genotyping of Staphylococcus epidermidis isolates by automated multilocus variable-number of tandem repeats: a tool for real-time epidemiology
    Patrice Francois
    University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 72:296-305. 2008
    ..This type of rapid and high-throughput methodology opens the possibility to rapidly assess long-term nosocomial transmission or to characterize infecting strains in the general procedure of routine laboratories, in real-time...
  11. pmc Reassessing the role of Staphylococcus aureus clumping factor and fibronectin-binding protein by expression in Lactococcus lactis
    Y A Que
    Division of Infectious Diseases, Department of Internal Medicine, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
    Infect Immun 69:6296-302. 2001
    ..Taken together, the present observations suggest that if antiadhesin therapy were to be developed, at least both of the clfA and fnbA products should be blocked for the therapy to be effective...
  12. doi request reprint Molecular diagnosis of bloodstream infections: planning to (physically) reach the bedside
    N Leggieri
    Bacteriology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, rue Gabrielle Perret Gentil, Geneva, Switzerland
    Curr Opin Infect Dis 23:311-9. 2010
    ....
  13. pmc Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 43:3346-55. 2005
    ..All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time...
  14. pmc Induction of fibronectin adhesins in quinolone-resistant Staphylococcus aureus by subinhibitory levels of ciprofloxacin or by sigma B transcription factor activity is mediated by two separate pathways
    Dongmei Li
    Service of Infectious Diseases, University Hospitals of Geneva, Geneva, Switzerland
    Antimicrob Agents Chemother 49:916-24. 2005
    ..aureus occurs via independent pathways whose concerted actions may significantly promote bacterial adhesion and colonization...
  15. pmc A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements
    Patrice Francois
    Genomic Research Laboratory, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 42:3309-12. 2004
    ..This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA...
  16. doi request reprint Risk factors for treatment failure in orthopedic device-related methicillin-resistant Staphylococcus aureus infection
    T Ferry
    Infectious Diseases Unit, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland
    Eur J Clin Microbiol Infect Dis 29:171-80. 2010
    ..2-16.3; P = 0.025) were independent predictors of treatment failure after debridement. Therapy using a combination of antimicrobials should be considered for patients with MRSA ODRI, especially when implant removal is not feasible...
  17. doi request reprint Molecular epidemiology of the nasal colonization by methicillin-susceptible Staphylococcus aureus in Swiss children
    C Mégevand
    Department of Paediatrics, University of Geneva Hospitals, Geneva, Switzerland
    Clin Microbiol Infect 16:1414-20. 2010
    ..Multilocus sequence typing confirmed this observation of diversity and identified ST45 as a frequent colonizer. This broad diversity in MSSA carriage strains suggests a limited selection pressure in our geographical area...
  18. doi request reprint Surveillance of extended-spectrum-beta-lactamase-producing Enterobacteriaceae in a Swiss Tertiary Care Hospital
    C Fankhauser
    Infection Control Programme, University of Geneva Hospitals, Geneva, Switzerland
    Swiss Med Wkly 139:747-51. 2009
    ....
  19. ncbi request reprint In vivo emergence of subpopulations expressing teicoplanin or vancomycin resistance phenotypes in a glycopeptide-susceptible, methicillin-resistant strain of Staphylococcus aureus
    P Vaudaux
    Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Antimicrob Chemother 47:163-70. 2001
    ..aureus subpopulations exhibiting decreased glycopeptide susceptibility and growing in the presence of otherwise inhibitory concentrations of these antimicrobial agents...
  20. pmc Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level
    Abdessalam Cherkaoui
    Clinical Microbiology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 48:1169-75. 2010
    ..Our data suggest that implementation of MS as a first test strategy for one-step species identification would improve timeliness and reduce isolate identification costs in clinical bacteriology laboratories now...
  21. pmc Use of oligoarrays for characterization of community-onset methicillin-resistant Staphylococcus aureus
    Thibaud Koessler
    Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH 1211 Geneva 14, Switzerland
    J Clin Microbiol 44:1040-8. 2006
    ....
  22. pmc Comparison of levofloxacin, alatrofloxacin, and vancomycin for prophylaxis and treatment of experimental foreign-body-associated infection by methicillin-resistant Staphylococcus aureus
    Pierre Vaudaux
    Division of Infectious Diseases, Geneva University Hospital, CH 1211 Geneva 14, Switzerland
    Antimicrob Agents Chemother 46:1503-9. 2002
    ..The mechanisms explaining the inferior activity of alatrofloxacin compared to the activity of levofloxacin against chronic foreign-body-associated infections by MRSA are unknown...
  23. pmc Increased expression of clumping factor and fibronectin-binding proteins by hemB mutants of Staphylococcus aureus expressing small colony variant phenotypes
    Pierre Vaudaux
    Division of Infectious Diseases, University Hospital, CH 1211 Geneva 14, Switzerland
    Infect Immun 70:5428-37. 2002
    ..Both agr-positive and agr-lacking hemB mutants were also more efficiently internalized by human embryonic kidney cells than were their isogenic controls, presumably because of increased surface display of their fibronectin adhesins...
  24. ncbi request reprint Evaluation of a high-density oligonucleotide array for characterization of grlA, grlB, gyrA and gyrB mutations in fluoroquinolone resistant Staphylococcus aureus isolates
    Sabine Couzinet
    Division of Infectious Diseases, University Hospitals of Geneva, CH 1211, 24 Rue Micheli du Crest, 1211 Geneva 14, Switzerland
    J Microbiol Methods 60:275-9. 2005
    ..For the two most important genes, gyrA and grlA, correlation with sequencing reached 95.1%. If all genes were considered, correlation was 88.8%...
  25. ncbi request reprint A recA-LexA-dependent pathway mediates ciprofloxacin-induced fibronectin binding in Staphylococcus aureus
    Carmelo Bisognano
    Division of Infectious Diseases, University Hospital, CH 1211 Geneva 14, Switzerland
    J Biol Chem 279:9064-71. 2004
    ..We conclude that activation of recA and derepression of lexA-regulated genes by CPX may represent a response to drug-induced damage that results in a novel induction of a virulence factor leading to increased bacterial tissue adherence...
  26. ncbi request reprint Comparative efficacy of daptomycin and vancomycin in the therapy of experimental foreign body infection due to Staphylococcus aureus
    Pierre Vaudaux
    Division of Infectious Diseases, Geneva University Hospital, CH 1211 Geneva 14, Switzerland
    J Antimicrob Chemother 52:89-95. 2003
    ..aureus. Drug dosage should be adapted to obtain inflammatory fluid levels of daptomycin minimizing emergence of resistant subpopulations...
  27. pmc Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-2005
    Patrice Francois
    University of Geneva Hospitals, Geneva, Switzerland
    Emerg Infect Dis 14:304-7. 2008
    ..We demonstrated strain diversity, including clones rarely found in Europe. Local epidemiology of community-associated MRSA is diverse and is evolving by importation and transmission of new strains...
  28. ncbi request reprint Proteomic approach to investigate MRSA
    Patrice Francois
    Department of Internal Medicine, Service of Infectious Diseases Genomic Research Laboratory, Geneva, Switzerland
    Methods Mol Biol 391:179-99. 2007
    ..aureus on the organism level. Coupled with methods analyzing the whole bacterial transcriptome, this approach might contribute to the development of rapid diagnostic tests and to the identification of new drug targets...
  29. ncbi request reprint Genome content determination in methicillin-resistant Staphylococcus aureus
    Patrice Francois
    Service of Infectious Diseases, Genomic Research Laboratory, Department of Internal Medicine, Geneva 14, Switzerland
    Future Microbiol 2:187-98. 2007
    ..In addition, sequence information is mandatory for the development of molecular tests allowing the rapid identification, genotyping and characterization of clinical isolates...
  30. doi request reprint Effects of amoxicillin treatment on the salivary microbiota in children with acute otitis media
    V Lazarevic
    Division of Infectious Diseases, Genomic Research Laboratory, Geneva University Hospitals, Geneva, Switzerland
    Clin Microbiol Infect 19:E335-42. 2013
    ..Our results point to a substantial but incomplete recovery of the salivary bacterial community from the antibiotic about 3 weeks after the end of treatment...
  31. doi request reprint New approaches for functional genomic studies in staphylococci
    Marie Beaume
    Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals HUG, CH 1211 Geneva 14, Switzerland
    Int J Med Microbiol 300:88-97. 2010
    ..Here we review the principles, advantages and disadvantages of recent technical developments in the field of transcriptomics and their potential impact on S. aureus research...
  32. ncbi request reprint High-density DNA probe arrays for identification of staphylococci to the species level
    Sabine Couzinet
    Genomic Research Laboratory, University Hospitals of Geneva Rue Micheli du Crest 24, 1211 Geneva 14, Switzerland
    J Microbiol Methods 61:201-8. 2005
    ..The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies...
  33. doi request reprint Development and evaluation of a rapid strategy to determine enterotoxin gene content in Staphylococcus aureus
    Adrien Fischer
    Genomic Research Laboratory, Service of Infectious Diseases, University Hospital of Geneva, University of Geneva, CH 1 211 Geneva 14, Switzerland
    J Microbiol Methods 77:184-90. 2009
    ..A combined genotyping method that includes rapid enterotoxin content determination appears informative for various epidemiological survey purposes...
  34. ncbi request reprint Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogens
    Patrice Francois
    Genomic Research Laboratory, Division of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 55:755-62. 2003
    ..Our study reveals that nanoparticle-labeled bacterial RNA generates reproducible resonance light scattering signals that are at least 50 times more intense than state-of-the-art confocal-based fluorescence signals...
  35. pmc Cartography of methicillin-resistant S. aureus transcripts: detection, orientation and temporal expression during growth phase and stress conditions
    Marie Beaume
    Genomic Research Laboratory, Infectious Diseases Service, Geneva University Hospitals, Geneva, Switzerland
    PLoS ONE 5:e10725. 2010
    ..Most studies evaluating gene content have so far neglected to analyze the so-called intergenic regions as well as potential antisense RNA molecules...
  36. ncbi request reprint Comparison of amplification methods for transcriptomic analyses of low abundance prokaryotic RNA sources
    Patrice Francois
    Genomic Research Laboratory, Service of Infectious Diseases, University Hospitals of Geneva, CH 1211 Geneva 14, Switzerland
    J Microbiol Methods 68:385-91. 2007
    ....
  37. ncbi request reprint Rapid bacterial identification using evanescent-waveguide oligonucleotide microarray classification
    Patrice Francois
    University Hospitals of Geneva, Genomic Research Laboratory, Service of Infectious Diseases, Switzerland
    J Microbiol Methods 65:390-403. 2006
    ..In conclusion, when coupled to optimal signal detection strategy, microarrays provide bacterial identification within a few hours post-sampling, allowing targeted antimicrobial prescription...
  38. pmc Novel microarray design strategy to study complex bacterial communities
    Antoine Huyghe
    Genomic Research Laboratory, Infectious Diseases Service, University of Geneva Hospitals, Micheli du Crest 24, 1211 Geneva 14, Geneva, Switzerland
    Appl Environ Microbiol 74:1876-85. 2008
    ..Our results show that this hierarchical approach can reveal minor changes (<or=1%) in gingival flora content when samples collected in individuals from similar geographical origins are compared...
  39. doi request reprint Ceftazidime-resistant Klebsiella oxytoca producing an OXY-2-type variant from Switzerland
    José Manuel Rodríguez-Martínez
    Int J Antimicrob Agents 32:278-9. 2008
  40. pmc In vivo detection of clonally derived methicillin-resistant/methicillin-susceptible Staphylococcus aureus strains is not a rare event
    Patrice Francois
    J Clin Microbiol 46:1890-1. 2008
  41. ncbi request reprint Compliance with guidelines on antibiotic prophylaxis in total hip replacement surgery: results of a retrospective study of 416 patients in a teaching hospital
    Pierrick Bedouch
    Unite d Evaluation Medicale, Centre Hospitalier Universitaire de Grenoble, France
    Infect Control Hosp Epidemiol 25:302-7. 2004
    ..To assess compliance of anesthesiologist practices in antibiotic prophylaxis during total hip replacement (THR) surgery with the French Society of Anesthesiology and Intensive Care consensus-based guidelines...
  42. ncbi request reprint Inactivation of a subpopulation of human neutrophils by exposure to ultrahigh-molecular-weight polyethylene wear debris
    Louis Bernard
    Service of Orthopaedic Surgery, Geneva University Hospital, Switzerland
    FEMS Immunol Med Microbiol 49:425-32. 2007
    ..In conclusion, engulfment of UHMWPE wear particles led to inactivation of S. aureus uptake in nearly half of the neutrophil population, which may potentially impair host clearance mechanisms against pyogenic infections...
  43. ncbi request reprint Nonredundant mass spectrometry: a strategy to integrate mass spectrometry acquisition and analysis
    Alexander Scherl
    Biomedical Proteomics Research Group, Central Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland
    Proteomics 4:917-27. 2004
    ..It was also found that this new workflow significantly increased sequence coverage by identifying unexpected peptides, which can result from post-translational modifications...
  44. ncbi request reprint Evidence of an intracellular reservoir in the nasal mucosa of patients with recurrent Staphylococcus aureus rhinosinusitis
    Sophie Clement
    Department of Pathology and Immunology, University of Geneva, Switzerland
    J Infect Dis 192:1023-8. 2005
    ..Intracellular residence may provide a sanctuary for pathogenic bacteria by protecting them from host defense mechanisms and antibiotic treatment during acute, recurrent S. aureus rhinosinusitis...
  45. pmc A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cells
    Christian Garzoni
    Service of Infectious Diseases, University Hospital of Geneva, Department of Internal Medicine, Geneva 14, Switzerland
    BMC Genomics 8:171. 2007
    ..Mechanisms contributing to internalization have been partly elucidated, but bacterial processes triggered intracellularly are largely unknown...