Britta Engelhardt

Summary

Affiliation: University of Bern
Country: Switzerland

Publications

  1. doi request reprint Differential roles for endothelial ICAM-1, ICAM-2, and VCAM-1 in shear-resistant T cell arrest, polarization, and directed crawling on blood-brain barrier endothelium
    Oliver Steiner
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    J Immunol 185:4846-55. 2010
  2. doi request reprint Capture, crawl, cross: the T cell code to breach the blood-brain barriers
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, CH 3012 Bern, Switzerland
    Trends Immunol 33:579-89. 2012
  3. pmc Claudin-1 induced sealing of blood-brain barrier tight junctions ameliorates chronic experimental autoimmune encephalomyelitis
    Friederike Pfeiffer
    Theodor Kocher Institute, University of Bern, Switzerland
    Acta Neuropathol 122:601-14. 2011
  4. pmc Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus
    Lena Wyss
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    PLoS ONE 7:e45619. 2012
  5. pmc Novel insights into the development and maintenance of the blood-brain barrier
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, 3012, Bern, Switzerland
    Cell Tissue Res 355:687-99. 2014
  6. pmc Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo
    Caroline Coisne
    Theodor Kocher Institute, University of Bern, Bern, 3012, Switzerland
    Fluids Barriers CNS 10:7. 2013
  7. ncbi request reprint Molecular mechanisms involved in T cell migration across the blood-brain barrier
    B Engelhardt
    Theodor Kocher Institute, University of Bern, Switzerland
    J Neural Transm 113:477-85. 2006
  8. doi request reprint Immune cell entry into the central nervous system: involvement of adhesion molecules and chemokines
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, CH 3012 Bern, Switzerland
    J Neurol Sci 274:23-6. 2008
  9. ncbi request reprint Natalizumab: targeting alpha4-integrins in multiple sclerosis
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Neurodegener Dis 5:16-22. 2008
  10. ncbi request reprint Therapeutic targeting of alpha 4-integrins in chronic inflammatory diseases: tipping the scales of risk towards benefit?
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, CH 3012 Bern, Switzerland
    Eur J Immunol 35:2268-73. 2005

Detail Information

Publications73

  1. doi request reprint Differential roles for endothelial ICAM-1, ICAM-2, and VCAM-1 in shear-resistant T cell arrest, polarization, and directed crawling on blood-brain barrier endothelium
    Oliver Steiner
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    J Immunol 185:4846-55. 2010
    ....
  2. doi request reprint Capture, crawl, cross: the T cell code to breach the blood-brain barriers
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, CH 3012 Bern, Switzerland
    Trends Immunol 33:579-89. 2012
    ..With a focus on multiple sclerosis (MS) and its animal models, this review summarizes the distinct molecular mechanisms required for immune cell migration across the different CNS barriers...
  3. pmc Claudin-1 induced sealing of blood-brain barrier tight junctions ameliorates chronic experimental autoimmune encephalomyelitis
    Friederike Pfeiffer
    Theodor Kocher Institute, University of Bern, Switzerland
    Acta Neuropathol 122:601-14. 2011
    ..Our observations suggest that stabilizing BBB barrier function by therapeutic targeting of TJs may be beneficial in treating MS, especially when anti-inflammatory treatments have failed...
  4. pmc Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus
    Lena Wyss
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    PLoS ONE 7:e45619. 2012
    ..Taken together, our study suggests that JAM-C(-/-) C57BL/6 mice model the important role for JAM-C in brain development and CSF homeostasis as recently observed in humans with a loss-of-function mutation in JAM-C...
  5. pmc Novel insights into the development and maintenance of the blood-brain barrier
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, 3012, Bern, Switzerland
    Cell Tissue Res 355:687-99. 2014
    ....
  6. pmc Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo
    Caroline Coisne
    Theodor Kocher Institute, University of Bern, Bern, 3012, Switzerland
    Fluids Barriers CNS 10:7. 2013
    ..abstract:..
  7. ncbi request reprint Molecular mechanisms involved in T cell migration across the blood-brain barrier
    B Engelhardt
    Theodor Kocher Institute, University of Bern, Switzerland
    J Neural Transm 113:477-85. 2006
    ..Here, I summarize our current knowledge on the sequence of traffic signals involved in T lymphocyte recruitment across the healthy and inflamed blood-brain and blood-spinal cord barrier based on our in vitro and in vivo investigations...
  8. doi request reprint Immune cell entry into the central nervous system: involvement of adhesion molecules and chemokines
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, CH 3012 Bern, Switzerland
    J Neurol Sci 274:23-6. 2008
    ....
  9. ncbi request reprint Natalizumab: targeting alpha4-integrins in multiple sclerosis
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Neurodegener Dis 5:16-22. 2008
    ..In this review, we discuss the basic mechanism of action, key clinical results of clinical trials and the emerging indication of natalizumab in MS...
  10. ncbi request reprint Therapeutic targeting of alpha 4-integrins in chronic inflammatory diseases: tipping the scales of risk towards benefit?
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, CH 3012 Bern, Switzerland
    Eur J Immunol 35:2268-73. 2005
    ..In order to properly evaluate the efficacy and safety of anti-alpha4-integrin therapy, the relationship between these observations in an immunologically altered animal model and human clinical disease needs to be carefully measured...
  11. ncbi request reprint The ins and outs of T-lymphocyte trafficking to the CNS: anatomical sites and molecular mechanisms
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, CH 3012 Bern, Switzerland
    Trends Immunol 26:485-95. 2005
    ..An update on findings from clinical trials of natalizumab is also provided...
  12. ncbi request reprint P-selectin glycoprotein ligand 1 is not required for the development of experimental autoimmune encephalomyelitis in SJL and C57BL/6 mice
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, CH 3012 Bern, Switzerland
    J Immunol 175:1267-75. 2005
    ..Taken together, our findings demonstrate that PSGL-1 is not required for the pathogenesis of EAE...
  13. ncbi request reprint The blood-central nervous system barriers actively control immune cell entry into the central nervous system
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestr 1, CH 3012 Bern, Switzerland
    Curr Pharm Des 14:1555-65. 2008
    ..Thus, immune cell entry into the CNS critically depends on the unique characteristics of the brain barriers maintaining CNS homeostasis...
  14. ncbi request reprint Mini-review: Transendothelial migration of leukocytes: through the front door or around the side of the house?
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Eur J Immunol 34:2955-63. 2004
    ..On the basis of the current experimental evidence, we propose consideration that both pathways are equally possible for a leukocyte's journey from the apical surface of the endothelium to its basal side...
  15. ncbi request reprint Regulation of immune cell entry into the central nervous system
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Switzerland
    Results Probl Cell Differ 43:259-80. 2006
    ....
  16. doi request reprint The blood-brain and the blood-cerebrospinal fluid barriers: function and dysfunction
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, 3012, Bern, Switzerland
    Semin Immunopathol 31:497-511. 2009
    ..In this review we will describe current knowledge on the cellular and molecular basis of the functional and dysfunctional blood-CNS barriers with focus on CNS autoimmune inflammation...
  17. pmc Fluids and barriers of the CNS establish immune privilege by confining immune surveillance to a two-walled castle moat surrounding the CNS castle
    Britta Engelhardt
    Theodor Kocher Institute, University of Bern, CH 3012 Bern, Switzerland
    Fluids Barriers CNS 8:4. 2011
    ....
  18. doi request reprint β2 integrin-mediated crawling on endothelial ICAM-1 and ICAM-2 is a prerequisite for transcellular neutrophil diapedesis across the inflamed blood-brain barrier
    Roser Gorina
    Theodor Kocher Institute, University of Bern, Bern CH 3012, Switzerland
    J Immunol 192:324-37. 2014
    ..Thus, β2 integrin-mediated neutrophil crawling on endothelial ICAM-1 and ICAM-2 is a prerequisite for transcellular neutrophil diapedesis across the inflamed BBB...
  19. ncbi request reprint E- and P-selectin are not required for the development of experimental autoimmune encephalomyelitis in C57BL/6 and SJL mice
    Axinia Döring
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    J Immunol 179:8470-9. 2007
    ..Thus, E- and P-selectin are not required for leukocyte recruitment across the BBB and the development of EAE in C57BL/6 and in SJL mice...
  20. pmc DARC shuttles inflammatory chemokines across the blood-brain barrier during autoimmune central nervous system inflammation
    Carsten Minten
    1 Theodor Kocher Institute, University of Bern, CH 3012 Bern, Switzerland
    Brain 137:1454-69. 2014
    ....
  21. doi request reprint Distinct molecular composition of blood and lymphatic vascular endothelial cell junctions establishes specific functional barriers within the peripheral lymph node
    Friederike Pfeiffer
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Eur J Immunol 38:2142-55. 2008
    ....
  22. pmc Comparison of immortalized bEnd5 and primary mouse brain microvascular endothelial cells as in vitro blood-brain barrier models for the study of T cell extravasation
    Oliver Steiner
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    J Cereb Blood Flow Metab 31:315-27. 2011
    ..However, because pMBMECs better reflect endothelial BBB specialization in vivo, we propose that more reliable information about the cellular and molecular mechanisms of T cell diapedesis across the BBB can be attained using pMBMECs...
  23. doi request reprint TET inducible expression of the α4β7-integrin ligand MAdCAM-1 on the blood-brain barrier does not influence the immunopathogenesis of experimental autoimmune encephalomyelitis
    Axinia Döring
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Eur J Immunol 41:813-21. 2011
    ..In conclusion, our study demonstrates that ectopic expression of MAdCAM-1 at the BBB does not increase α4β7-integrin-mediated immune cell trafficking into the CNS during MOG(aa35-55)-induced EAE...
  24. pmc Comprehensive analysis of lymph node stroma-expressed Ig superfamily members reveals redundant and nonredundant roles for ICAM-1, ICAM-2, and VCAM-1 in lymphocyte homing
    Rémy T Boscacci
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, Bern, Switzerland
    Blood 116:915-25. 2010
    ....
  25. doi request reprint Cutting edge: Natalizumab blocks adhesion but not initial contact of human T cells to the blood-brain barrier in vivo in an animal model of multiple sclerosis
    Caroline Coisne
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    J Immunol 182:5909-13. 2009
    ....
  26. doi request reprint Inducible endothelial cell-specific gene expression in transgenic mouse embryos and adult mice
    Urban Deutsch
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Exp Cell Res 314:1202-16. 2008
    ..These transgenic model systems provide versatile tools for the TET-regulated manipulation of endothelial cell-specific gene expression in the entire embryonic vasculature and distinct vascular beds in adult mice...
  27. doi request reprint Preclinical testing of strategies for therapeutic targeting of human T-cell trafficking in vivo
    Caroline Coisne
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Methods Mol Biol 616:268-81. 2010
    ....
  28. doi request reprint Culture-induced changes in blood-brain barrier transcriptome: implications for amino-acid transporters in vivo
    Ruth Lyck
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    J Cereb Blood Flow Metab 29:1491-502. 2009
    ..We hypothesized that the AA transporters highly expressed in ppMBMECs and downregulated in culture have a major in vivo function for BBB transendothelial transport...
  29. ncbi request reprint Therapeutic targeting of leukocyte trafficking across the blood-brain barrier
    Caroline Coisne
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, CH 3012 Bern, Switzerland
    Inflamm Allergy Drug Targets 6:210-22. 2007
    ..Other mechanisms of immune cell entry into the CNS might therefore apply under different pathological conditions such as bacterial meningitis or stroke and need to be considered...
  30. pmc The neurovascular unit as a selective barrier to polymorphonuclear granulocyte (PMN) infiltration into the brain after ischemic injury
    Gaby Enzmann
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, 3012, Bern, Switzerland
    Acta Neuropathol 125:395-412. 2013
    ..Our observations identify the NVU rather than the brain parenchyma as the site of PMN action after CNS ischemia and suggest reappraisal of targets for therapies to reduce reperfusion injury after stroke...
  31. doi request reprint PSGL-1 is dispensible for the development of active experimental autoimmune encephalomyelitis in SJL/J mice
    Ruben Bill
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, Bern, Switzerland
    J Neuroimmunol 232:207-8. 2011
    ..Here, we demonstrate that PSGL-1(-/-) SJL/J mice exhibited EAE pathogenesis indistinguishable from wild-type SJL/J mice. Our present study underscores and emphasizes previous observations that PSGL-1 is dispensible for EAE pathogenesis...
  32. doi request reprint Tight junctions in brain barriers during central nervous system inflammation
    Caroline Coisne
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Antioxid Redox Signal 15:1285-303. 2011
    ....
  33. ncbi request reprint Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation
    Esther Steiner
    Theodor Kocher Institute, University of Bern, CH 3012 Bern, Switzerland
    Glia 60:1646-59. 2012
    ..Reduced DG protein levels and redistribution of AQP4 as observed upon tMCAO might therefore counteract early edema formation and reflect a beneficial mechanism operating in the brain to minimize damage upon ischemia...
  34. doi request reprint Going against the tide--how encephalitogenic T cells breach the blood-brain barrier
    Ruth Lyck
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    J Vasc Res 49:497-509. 2012
    ..In this review, we will summarize the cellular and molecular mechanisms involved in the unique T cell behavior that is obviously required for finding a site permissive for diapedesis across the unique vascular bed of the BBB...
  35. doi request reprint L-Selectin-deficient SJL and C57BL/6 mice are not resistant to experimental autoimmune encephalomyelitis
    Chiara Uboldi
    Theodor Kocher Institute, University of Bern, Bern, Switzerland
    Eur J Immunol 38:2156-67. 2008
    ..PL mice, may be attributed to background genes rather than L-selectin and to a unique role of L-selectin in EAE pathogenesis in MBP-TCR-transgenic mice...
  36. pmc Flotillins interact with PSGL-1 in neutrophils and, upon stimulation, rapidly organize into membrane domains subsequently accumulating in the uropod
    Jérémie Rossy
    Department of Pathology, University of Bern, Bern, Switzerland
    PLoS ONE 4:e5403. 2009
    ..It was the goal of this study to investigate dynamic membrane microdomain reorganization during neutrophil activation...
  37. pmc Multistep nature of microvascular recruitment of ex vivo-expanded embryonic endothelial progenitor cells during tumor angiogenesis
    Peter Vajkoczy
    Department of Neurosurgery, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany
    J Exp Med 197:1755-65. 2003
    ..This paper provides the first in vivo insights into the mechanisms of endothelial progenitor cell recruitment and, thus, indicates novel ways to interfere with pathological neovascularization...
  38. doi request reprint Enhanced T cell transmigration across the murine liver sinusoidal endothelium is mediated by transcytosis and surface presentation of chemokines
    Arnhild Schrage
    Medizinische Klinik I, Campus Charité Benjamin Franklin, Berlin, Germany
    Hepatology 48:1262-72. 2008
    ..Blockage of transcytosis in LSEC significantly inhibited this effect, and this suggested that chemokines taken up from the basolateral side and presented on the luminal side of endothelial cells trigger T cell transmigration...
  39. ncbi request reprint Brain endothelial PPARgamma controls inflammation-induced CD4+ T cell adhesion and transmigration in vitro
    Luisa Klotz
    Institute of Molecular Medicine and Experimental Immunology, University of Bonn, Sigmund Freud Strasse 25, Bonn, Germany
    J Neuroimmunol 190:34-43. 2007
    ..We therefore propose that PPARgamma in brain EC may be exploited to target detrimental EC-T cell interactions under inflammatory conditions...
  40. ncbi request reprint Migration of immature mouse DC across resting endothelium is mediated by ICAM-2 but independent of beta2-integrins and murine DC-SIGN homologues
    Klaus Wethmar
    Max Planck Institute for Molecular Biomedicine, Munster, Germany, and Institute of Cell Biology, ZMBE, University of Munster, Munster, Germany
    Eur J Immunol 36:2781-94. 2006
    ..Taken together, this study shows that ICAM-2 strongly supports transmigration of immature DC across resting endothelium by interacting with ligands that are distinct from beta2-integrins and DC-SIGN homologues...
  41. ncbi request reprint Astrocyte mediated modulation of blood-brain barrier permeability does not correlate with a loss of tight junction proteins from the cellular contacts
    Stefan Hamm
    Max Planck Institute for Physiological and Clinical Research, Bad Nauheim, Germany
    Cell Tissue Res 315:157-66. 2004
    ..Thus, opening of BBB TJs is not readily accompanied by the complete loss of the junctional localization of TJ proteins...
  42. ncbi request reprint Detection of endothelial/lymphocyte interaction in spinal cord microvasculature by intravital videomicroscopy
    Britta Engelhardt
    Max Planck Institute for Physiological and Clinical Research, Bad Neuheim, Germany
    Methods Mol Med 89:83-93. 2003
  43. ncbi request reprint Development of the blood-brain barrier
    Britta Engelhardt
    Max Planck Institute for Vascular Biology, Von Esmarch Strasse 56, 48149, Munster, Germany
    Cell Tissue Res 314:119-29. 2003
    ..This review summarizes our current knowledge of the cellular and molecular mechanisms involved in the development and maintenance of the BBB...
  44. pmc Intracellular domain of brain endothelial intercellular adhesion molecule-1 is essential for T lymphocyte-mediated signaling and migration
    John Greenwood
    Division of Cell Biology, Institute of Ophthalmology, University College London, London, United Kingdom
    J Immunol 171:2099-108. 2003
    ..Thus, agents that mimic the ICAM-1 intracellular domain may be attractive targets for novel anti-inflammatory therapeutics...
  45. ncbi request reprint T-cell interaction with ICAM-1/ICAM-2 double-deficient brain endothelium in vitro: the cytoplasmic tail of endothelial ICAM-1 is necessary for transendothelial migration of T cells
    Ruth Lyck
    Max Planck Institute for Physiological and Clinical Research, W G Kerckhoff Institute, Bad Neuheim, Germany
    Blood 102:3675-83. 2003
    ..Thus, our data demonstrate that the cytoplasmic tail of endothelial ICAM-1-independently from tyrosine phosphorylation-is essential for supporting TEM of T lymphocytes, while Rho signaling is involved in endothelial cells...
  46. ncbi request reprint Gene and protein expression profiling of human cerebral endothelial cells activated with tumor necrosis factor-alpha
    Bo Franzén
    Gene and Protein Technology, Molecular Sciences, Local Discovery Research Area, CNS and Pain Control, AstraZeneca R and D, Sodertalje, Sweden
    Brain Res Mol Brain Res 115:130-46. 2003
    ..Our results indicate involvements of the urokinase plasminogen activator system and cytoskeletal rearrangements unique to TNFalpha activation of cerebral endothelial cells...
  47. ncbi request reprint Localization of claudin-3 in tight junctions of the blood-brain barrier is selectively lost during experimental autoimmune encephalomyelitis and human glioblastoma multiforme
    Hartwig Wolburg
    Institute for Pathology, University of Tubingen, Liebermeisterstrasse 8, 72076, Tubingen, Germany
    Acta Neuropathol 105:586-92. 2003
    ....
  48. ncbi request reprint Encephalitogenic T cells use LFA-1 for transendothelial migration but not during capture and initial adhesion strengthening in healthy spinal cord microvessels in vivo
    Melanie Laschinger
    Max Planck Institute for Physiological and Clinical Research, Bad Nauheim, Germany
    Eur J Immunol 32:3598-606. 2002
    ..Our study provides the first direct evidence that encephalitogenic T cells use LFA-1 for transendothelial migration but not for capture and initial adhesion in spinal cord microvessels in vivo...
  49. ncbi request reprint Isolation and molecular characterization of brain microvascular endothelial cells from human brain tumors
    Ronald E Unger
    Institute of Pathology, Uni Klinik Mainz, University of Mainz, Langenbeckstrasse 1, Germany
    In Vitro Cell Dev Biol Anim 38:273-81. 2002
    ....
  50. ncbi request reprint Glycosylation processing inhibition by castanospermine prevents experimental autoimmune encephalomyelitis by interference with IL-2 receptor signal transduction
    Silke Walter
    Department of Neurology, University of Gottingen, Robert Koch Str 40, D 37075 Gottingen, Germany
    J Neuroimmunol 132:1-10. 2002
    ..This relatively selective pharmacological effect of CAST highlights its potential as a novel immunomodulatory approach in multiple sclerosis...
  51. ncbi request reprint Functional expression of the lymphoid chemokines CCL19 (ELC) and CCL 21 (SLC) at the blood-brain barrier suggests their involvement in G-protein-dependent lymphocyte recruitment into the central nervous system during experimental autoimmune encephalomyeli
    Carsten Alt
    Max Planck Institute for Physiological and Clinical Research, W G Kerckhoff Institute, Department of Vascular Cell Biology, Bad Nauheim, Germany
    Eur J Immunol 32:2133-44. 2002
    ....
  52. ncbi request reprint A transmembrane tight junction protein selectively expressed on endothelial cells and platelets
    Ines Nasdala
    Institute of Cell Biology, ZMBE, University of Munster, 48149 Munster, Germany
    J Biol Chem 277:16294-303. 2002
    ..The 1G8 antigen supports homophilic interactions on transfected Chinese hamster ovary cells. Based on the similarity to the JAM molecules, it is plausible that the 1G8 antigen might be involved in interendothelial cell adhesion...
  53. ncbi request reprint Mouse CD99 participates in T-cell recruitment into inflamed skin
    Gabriele Bixel
    Max Planck Institute of Molecular Biomedicine, Von Esmarch Str 56, D 48149 Munster, Germany
    Blood 104:3205-13. 2004
    ..We conclude that mouse CD99 participates in the TEM of lymphocytes and in their recruitment to inflamed skin in vivo. This establishes CD99 as a valid target for interference with cutaneous inflammatory processes...
  54. pmc Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1-deficient mice
    Donnasue Graesser
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA
    J Clin Invest 109:383-92. 2002
    ..Lastly, we demonstrate an exaggerated and prolonged CNS vascular permeability during the development of EAE and a delay in restoration of dermal vascular integrity following histamine challenge in PECAM-KO mice...
  55. ncbi request reprint Polyoma virus middle-T-transformed PECAM-1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice
    Terri A Rothermel
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, Wisconsin 53792 4673, USA
    J Cell Physiol 202:230-9. 2005
    ....
  56. ncbi request reprint Dysferlin is a new marker for leaky brain blood vessels in multiple sclerosis
    Sonja Hochmeister
    Division of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria
    J Neuropathol Exp Neurol 65:855-65. 2006
    ..Hence, dysferlin is not only a marker for leaky brain vessels, but also reveals dissociation of perivascular inflammatory infiltrates and blood-brain barrier disturbance in multiple sclerosis...
  57. ncbi request reprint Diapedesis of mononuclear cells across cerebral venules during experimental autoimmune encephalomyelitis leaves tight junctions intact
    Hartwig Wolburg
    Institute for Pathology, University of Tubingen, Liebermeisterstrasse 8, 72076, Tubingen, Germany
    Acta Neuropathol 109:181-90. 2005
    ....
  58. ncbi request reprint The LPS receptor, CD14, in experimental autoimmune encephalomyelitis and multiple sclerosis
    Silke Walter
    Department of Neurology, Saarland University Hospital, Homburg, Germany
    Cell Physiol Biochem 17:167-72. 2006
    ..Thus, the key innate immune receptor, CD14, may be of pathophysiological relevance in experimental autoimmune encephalomyelitis and multiple sclerosis...
  59. ncbi request reprint A mechanosensory complex that mediates the endothelial cell response to fluid shear stress
    Eleni Tzima
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 437:426-31. 2005
    ..Therefore, this mechanosensing pathway is required for the earliest-known events in atherogenesis...
  60. doi request reprint Improving outcome after stroke: overcoming the translational roadblock
    Matthias Endres
    Department of Neurology, Center for Stroke Research, Charite, Berlin, Germany
    Cerebrovasc Dis 25:268-78. 2008
    ..These 5 areas are linked on all levels and therefore need to be tackled by an integrative approach and innovative therapeutic strategies...
  61. ncbi request reprint Alpha4 integrins as therapeutic targets in autoimmune disease
    Ulrich H von Andrian
    N Engl J Med 348:68-72. 2003
  62. ncbi request reprint Stroke research priorities for the next decade--A representative view of the European scientific community
    Stephen Meairs
    Neurologische Klinik, Ruprecht Karls Universitat Heidelberg, Theodor Kutzer Ufer, DE 68135 Mannheim Germany
    Cerebrovasc Dis 22:75-82. 2006
  63. ncbi request reprint Strategies to advance translational research into brain barriers
    Edward Neuwelt
    Oregon Health and Science University, Department of Neurology, Veterans Administration Medical Center, Portland, OR, USA
    Lancet Neurol 7:84-96. 2008
    ..At a recent meeting of expert panels, essential and high-priority recommendations to propel brain barrier research forward in six topical areas were developed and these recommendations are presented here...
  64. ncbi request reprint Direct evidence that polysorbate-80-coated poly(butylcyanoacrylate) nanoparticles deliver drugs to the CNS via specific mechanisms requiring prior binding of drug to the nanoparticles
    Jorg Kreuter
    Institut fur Pharmazeutische Technologie, Biozentrum, J W Goethe Universitat, Frankfurt, Germany
    Pharm Res 20:409-16. 2003
    ..The purpose of this study is to explore more fully the mechanism by which PBCA nanoparticles can deliver drugs to the brain...
  65. doi request reprint Effect of interferon-beta and atorvastatin on Th1/Th2 cytokines in multiple sclerosis
    Johann Sellner
    Department of Neurology, Inselspital, Bern University Hospital, and University of Bern, Switzerland
    Neurochem Int 53:17-21. 2008
    ..Supplemental atorvastatin might promote a Th1-type response by raising IL-12p70. Further studies are required to support a Th2 cytokine shift by atorvastatin in patients with MS...
  66. ncbi request reprint Building up the blood-brain barrier
    Peter Rieckmann
    Nat Med 9:828-9. 2003
  67. ncbi request reprint Angiostatin overexpression in Morris hepatoma results in decreased tumor growth but increased perfusion and vascularization
    Kerstin Schmidt
    Department of Anatomy and Cell Biology III, University of Heidelberg, INF 307, Heidelberg, Germany
    J Nucl Med 47:543-51. 2006
    ..Human angiostatin (hANG) is one of the most potent inhibitors of endothelial cell proliferation, angiogenesis, and tumor growth in vivo. However, its mechanisms operating in vivo are not well understood...
  68. ncbi request reprint Platelet endothelial cell adhesion molecule-1 modulates endothelial cell motility through the small G-protein Rho
    Dita Gratzinger
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    FASEB J 17:1458-69. 2003
    ..PECAM-1 may thus support the effective S1P/RhoGTP signaling required for wound healing endothelial migration by allowing for the spatially directed, coordinated activation of Galpha signaling pathways...
  69. pmc Importance of integrin LFA-1 deactivation for the generation of immune responses
    Monika Semmrich
    Department of Surgery, Immunology, and Hygiene, Technische Universitat Munchen, 81675 Munich, Germany
    J Exp Med 201:1987-98. 2005
    ..Thus, deactivation of LFA-1 and disassembly of LFA-1-mediated cell contacts seem to be vital for the generation of normal immune responses...
  70. ncbi request reprint Transfer of the sFLT-1 gene in Morris hepatoma results in decreased growth and perfusion and induction of genes associated with stress response
    Kerstin Schmidt
    Department of Anatomy and Cell Biology III, University of Heidelberg, Im Neuenheimer Feld 400, Heidelberg 69120, Germany
    Clin Cancer Res 11:2132-40. 2005
    ..Therefore, monitoring of antiangiogenic approaches with functional imaging and histomorphometrical analyses are desirable to evaluate the biological effects caused by this treatment modality...
  71. ncbi request reprint Gene and protein expression profiling of the microvascular compartment in experimental autoimmune encephalomyelitis in C57Bl/6 and SJL mice
    Carsten Alt
    Max Planck Institute for Molecular Biomedicine, Munster, Germany
    Brain Pathol 15:1-16. 2005
    ..Thus, proteomics and gene array screens of the microvascular compartment are valid approaches, that can be used to define novel candidate molecules involved in EAE pathogenesis at the level of the BBB...
  72. ncbi request reprint Interferon-beta 1b leads to a short-term increase of soluble but long-term stabilisation of cell surface bound adhesion molecules in multiple sclerosis
    Joerg Kraus
    Justus Liebig University of Giessen, Dept of Neurology, 35385 Giessen, Germany
    J Neurol 251:464-72. 2004
    ..Stabilisation of the expression of cell surface bound AMs on blood MNC may indicate the beneficial effects of IFN-beta1b therapy in MS patients...
  73. ncbi request reprint Interferon-beta stabilizes barrier characteristics of brain endothelial cells in vitro
    Jorg Kraus
    Max Planck Institute for Physiological and Clinical Research, Department of Vascular Cell Biology, Bad Nauheim, Germany
    Ann Neurol 56:192-205. 2004
    ..Taken together, our data demonstrate a direct stabilizing effect of IFN-beta on BBB cerebral endothelial cells in vitro that might significantly contribute to the beneficial effects of IFN-beta treatment in MS in vivo...