Raimund Dutzler

Summary

Affiliation: University of Zurich
Country: Switzerland

Publications

  1. ncbi Translocation mechanism of long sugar chains across the maltoporin membrane channel
    Raimund Dutzler
    Department of Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Structure 10:1273-84. 2002
  2. ncbi The ClC family of chloride channels and transporters
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Curr Opin Struct Biol 16:439-46. 2006
  3. ncbi The structure of the cytoplasmic domain of the chloride channel ClC-Ka reveals a conserved interaction interface
    Sandra Markovic
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Structure 15:715-25. 2007
  4. doi Structural basis of open channel block in a prokaryotic pentameric ligand-gated ion channel
    Ricarda J C Hilf
    Department of Biochemistry, University of Zurich, Zurich, Switzerland
    Nat Struct Mol Biol 17:1330-6. 2010
  5. ncbi Structural basis for ion conduction and gating in ClC chloride channels
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    FEBS Lett 564:229-33. 2004
  6. ncbi The structural basis of ClC chloride channel function
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Trends Neurosci 27:315-20. 2004
  7. ncbi A structural perspective on ClC channel and transporter function
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    FEBS Lett 581:2839-44. 2007
  8. doi Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel
    Ricarda J C Hilf
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Nature 457:115-8. 2009
  9. pmc Inhibition of the prokaryotic pentameric ligand-gated ion channel ELIC by divalent cations
    Iwan Zimmermann
    Department of Biochemistry, University of Zurich, Zurich, Switzerland
    PLoS Biol 10:e1001429. 2012
  10. doi X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter
    Stefan Warmuth
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Structure 17:538-46. 2009

Collaborators

Detail Information

Publications23

  1. ncbi Translocation mechanism of long sugar chains across the maltoporin membrane channel
    Raimund Dutzler
    Department of Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Structure 10:1273-84. 2002
    ..Thus, maltoporin functions like an efficient translocation "enzyme," and the slow rate of the register shift (approximately 1/ms) is due to high collisional friction...
  2. ncbi The ClC family of chloride channels and transporters
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Curr Opin Struct Biol 16:439-46. 2006
    ..The conservation in the structure and the functional resemblance in gating and transport mechanism suggest a strong mechanistic relationship between seemingly contradictory transport modes...
  3. ncbi The structure of the cytoplasmic domain of the chloride channel ClC-Ka reveals a conserved interaction interface
    Sandra Markovic
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Structure 15:715-25. 2007
    ..Our results reveal a conserved interaction interface that relates the cytoplasmic domains of ClC proteins and establish a structural relationship that is likely general for this important family of transport proteins...
  4. doi Structural basis of open channel block in a prokaryotic pentameric ligand-gated ion channel
    Ricarda J C Hilf
    Department of Biochemistry, University of Zurich, Zurich, Switzerland
    Nat Struct Mol Biol 17:1330-6. 2010
    ..The results provide a structural foundation for understanding the interactions of the channel with inhibitors that is relevant for the entire family...
  5. ncbi Structural basis for ion conduction and gating in ClC chloride channels
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurerstr 190, CH 8057 Zurich, Switzerland
    FEBS Lett 564:229-33. 2004
    ..Mutations of this glutamate residue in Torpedo ray ClC channels alter gating in electrophysiological assays. These findings reveal a form of gating in which the glutamate carboxyl group closes the pore by mimicking a Cl(-) ion...
  6. ncbi The structural basis of ClC chloride channel function
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Trends Neurosci 27:315-20. 2004
    ..In the closed conformation of the channel, a conserved glutamate residue occupies one of the ion-binding sites; on channel opening, this glutamate residue moves out of the binding site, thereby making room for an additional Cl(-) ion...
  7. ncbi A structural perspective on ClC channel and transporter function
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    FEBS Lett 581:2839-44. 2007
    ....
  8. doi Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel
    Ricarda J C Hilf
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Nature 457:115-8. 2009
    ..In combination, both structures suggest a novel gating mechanism for pentameric ligand-gated ion channels where channel opening proceeds by a change in the tilt of the pore-forming helices...
  9. pmc Inhibition of the prokaryotic pentameric ligand-gated ion channel ELIC by divalent cations
    Iwan Zimmermann
    Department of Biochemistry, University of Zurich, Zurich, Switzerland
    PLoS Biol 10:e1001429. 2012
    ..Our study provides structural and functional insight into the allosteric regulation of ELIC and is of potential relevance for the entire family...
  10. doi X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter
    Stefan Warmuth
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Structure 17:538-46. 2009
    ..Our studies reveal structural insight into the CCC family and establish the oligomeric organization of this important class of transport proteins...
  11. pmc Functional characterization of a ClC transporter by solid-supported membrane electrophysiology
    Juan Garcia-Celma
    Department of Biochemistry, University of Zurich, CH 8057 Zurich, Switzerland
    J Gen Physiol 141:479-91. 2013
    ..Our results have provided a quantitative basis for the transport behavior of EcClC, and they will serve as a reference for future investigations of novel electrogenic transporters with still-uncharacterized properties...
  12. ncbi Nucleotide recognition by the cytoplasmic domain of the human chloride transporter ClC-5
    Sebastian Meyer
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Nat Struct Mol Biol 14:60-7. 2007
    ..Our results establish the structural and energetic basis for the interaction of ClC-5 with nucleotides and provide a framework for future investigations...
  13. ncbi Crystal structure of the cytoplasmic domain of the chloride channel ClC-0
    Sebastian Meyer
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Structure 14:299-307. 2006
    ..The structure provides insight into the organization of the cytoplasmic domains within the ClC family and establishes a framework for guiding future investigations on regulatory mechanisms...
  14. doi X-ray structure of a prokaryotic pentameric ligand-gated ion channel
    Ricarda J C Hilf
    Department of Biochemistry, University of Zurich, Winterthurer Strasse 190, CH 8057 Zurich, Switzerland
    Nature 452:375-9. 2008
    ..3 A resolution. Our study reveals the first structure of a pLGIC at high resolution and provides an important model system for the investigation of the general mechanisms of ion permeation and gating within the family...
  15. pmc Ligand activation of the prokaryotic pentameric ligand-gated ion channel ELIC
    Iwan Zimmermann
    Department of Biochemistry, University of Zurich, Zurich, Switzerland
    PLoS Biol 9:e1001101. 2011
    ..The high single channel conductance and the comparably simple functional behavior make ELIC an attractive model system to study general mechanisms of ion conduction and gating in this important family of neurotransmitter receptors...
  16. doi A prokaryotic perspective on pentameric ligand-gated ion channel structure
    Ricarda Jc Hilf
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Curr Opin Struct Biol 19:418-24. 2009
    ..The two structures thus suggest a novel gating mechanism for the family, where pore opening proceeds by a change of the tilt of the pore-forming helices...
  17. ncbi Channels and transporters. Mini-symposium of the Division of Medicinal Chemistry (DMC) of the Swiss Chemical Society (SCS) at the Department of Chemistry, University of Basel, May 27, 2010
    Raimund Dutzler
    Department of Biochemistry, University of Zurich, CH 8057 Zurich
    Chimia (Aarau) 64:662-6. 2010
    ..transporters ('The Structural Basis for Ion Conduction and Gating in Pentameric Ligand-Gated Ion Channels' by Raimund Dutzler and 'Uptake and Efflux Transporters for Endogenous Substances and for Drugs' by Dietrich Keppler), and a case ..
  18. pmc Ion-binding properties of the ClC chloride selectivity filter
    Séverine Lobet
    Department of Biochemistry, University of Zurich, Zurich, Switzerland
    EMBO J 25:24-33. 2006
    ..In resemblance to K+ channels, ions permeate the ClC channel in a single file, with mutual repulsion between the ions fostering rapid conduction...
  19. doi A versatile and efficient high-throughput cloning tool for structural biology
    Eric R Geertsma
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Biochemistry 50:3272-8. 2011
    ..It considerably speeds up the generation of expression constructs compared to traditional methods and thus facilitates a broader expression screening...
  20. ncbi The cytoplasmic domain of the chloride channel ClC-0: structural and dynamic characterization of flexible regions
    Simon Alioth
    Laboratorium fur Physikalische Chemie, ETH Zurich, CH 8093 Zurich, Switzerland
    J Mol Biol 369:1163-9. 2007
    ..This study characterizes for the first time the biophysical properties of these protein segments, which may become important for the understanding of novel regulatory mechanisms within the ClC family...
  21. ncbi Synergism between halide binding and proton transport in a CLC-type exchanger
    Alessio Accardi
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454 9110, USA
    J Mol Biol 362:691-9. 2006
    ....
  22. ncbi Gating the selectivity filter in ClC chloride channels
    Raimund Dutzler
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 300:108-12. 2003
    ..Mutations of this glutamate residue in Torpedo ray ClC channels alter gating in electrophysiological assays. These findings reveal a form of gating in which the glutamate carboxyl group closes the pore by mimicking a Cl- ion...
  23. ncbi X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity
    Raimund Dutzler
    Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 415:287-94. 2002
    ..These findings provide a structural basis for further understanding the function of ClC Cl- channels, and establish the physical and chemical basis of their anion selectivity...