Nicolas Demaurex

Summary

Affiliation: University of Geneva
Country: Switzerland

Publications

  1. doi request reprint Dual effect of cell-cell contact disruption on cytosolic calcium and insulin secretion
    Fabienne Jaques
    Department of Genetic Medicine and Development, University of Geneva Medical Center, 1211 Geneva 4, Switzerland
    Endocrinology 149:2494-505. 2008
  2. pmc Interactions between electron and proton currents in excised patches from human eosinophils
    Gábor L Petheo
    Department of Physiology, University of Geneva Medical Center, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    J Gen Physiol 122:713-26. 2003
  3. ncbi request reprint Calcium measurements in organelles with Ca2+-sensitive fluorescent proteins
    Nicolas Demaurex
    Department of Cell Physiology and Metabolism, University of Geneva, 1 rue Michel Servet, CH 1211 Geneva 4, Switzerland
    Cell Calcium 38:213-22. 2005
  4. ncbi request reprint Measurements of the free luminal ER Ca(2+) concentration with targeted "cameleon" fluorescent proteins
    Nicolas Demaurex
    Department of Physiology, University of Geneva Medical Center, 1, Michel Servet, CH 1211 4, Geneva, Switzerland
    Cell Calcium 34:109-19. 2003
  5. pmc Electron and proton transport by NADPH oxidases
    Nicolas Demaurex
    University of Geneva Department of Cell Physiology and Metabolism 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    Philos Trans R Soc Lond B Biol Sci 360:2315-25. 2005
  6. ncbi request reprint Cell biology. Apoptosis--the calcium connection
    Nicolas Demaurex
    Department of Physiology, University of Geneva Medical Center, Geneva, Switzerland
    Science 300:65-7. 2003
  7. ncbi request reprint Ca(2+) homeostasis during mitochondrial fragmentation and perinuclear clustering induced by hFis1
    Maud Frieden
    Department of Physiology, University of Geneva Medical Center, Geneva 4, Switzerland
    J Biol Chem 279:22704-14. 2004
  8. ncbi request reprint Subplasmalemmal mitochondria modulate the activity of plasma membrane Ca2+-ATPases
    Maud Frieden
    Department of Cell Physiology and Metabolism, University of Geneva Medical School, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    J Biol Chem 280:43198-208. 2005
  9. doi request reprint SLP-2 negatively modulates mitochondrial sodium-calcium exchange
    Sandrine Da Cruz
    Department of Cell Biology, University of Geneva, Switzerland
    Cell Calcium 47:11-8. 2010
  10. doi request reprint Do Hv1 proton channels regulate the ionic and redox homeostasis of phagosomes?
    Antoun El Chemaly
    Department of Cell Physiology and Metabolism, University of Geneva, 1211 Geneva 4, Switzerland
    Mol Cell Endocrinol 353:82-7. 2012

Collaborators

Detail Information

Publications52

  1. doi request reprint Dual effect of cell-cell contact disruption on cytosolic calcium and insulin secretion
    Fabienne Jaques
    Department of Genetic Medicine and Development, University of Geneva Medical Center, 1211 Geneva 4, Switzerland
    Endocrinology 149:2494-505. 2008
    ....
  2. pmc Interactions between electron and proton currents in excised patches from human eosinophils
    Gábor L Petheo
    Department of Physiology, University of Geneva Medical Center, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    J Gen Physiol 122:713-26. 2003
    ..The strict correlation between Ie and IH amplitudes and the sensitivity of IH to oxidase-specific agents suggest that the proton channel is either part of the oxidase complex or linked by a membrane-limited mediator...
  3. ncbi request reprint Calcium measurements in organelles with Ca2+-sensitive fluorescent proteins
    Nicolas Demaurex
    Department of Cell Physiology and Metabolism, University of Geneva, 1 rue Michel Servet, CH 1211 Geneva 4, Switzerland
    Cell Calcium 38:213-22. 2005
    ..In this chapter, I will review the progress made in the design and use of genetic Ca2+ indicators, and discuss how these new tools provide an opportunity to challenge several unsolved questions in Ca2+ signaling...
  4. ncbi request reprint Measurements of the free luminal ER Ca(2+) concentration with targeted "cameleon" fluorescent proteins
    Nicolas Demaurex
    Department of Physiology, University of Geneva Medical Center, 1, Michel Servet, CH 1211 4, Geneva, Switzerland
    Cell Calcium 34:109-19. 2003
    ..Each approach offers unique advantages and suffers from specific drawbacks. In this review, we will discuss the advantages and pitfalls of using the genetically encoded "cameleon" Ca(2+) indicators for ER Ca(2+) measurements...
  5. pmc Electron and proton transport by NADPH oxidases
    Nicolas Demaurex
    University of Geneva Department of Cell Physiology and Metabolism 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    Philos Trans R Soc Lond B Biol Sci 360:2315-25. 2005
    ....
  6. ncbi request reprint Cell biology. Apoptosis--the calcium connection
    Nicolas Demaurex
    Department of Physiology, University of Geneva Medical Center, Geneva, Switzerland
    Science 300:65-7. 2003
  7. ncbi request reprint Ca(2+) homeostasis during mitochondrial fragmentation and perinuclear clustering induced by hFis1
    Maud Frieden
    Department of Physiology, University of Geneva Medical Center, Geneva 4, Switzerland
    J Biol Chem 279:22704-14. 2004
    ..These observations indicate that close contact to Ca(2+) influx channels is not required for CCE modulation and that the formation of a mitochondrial network facilitates Ca(2+) propagation within interconnected mitochondria...
  8. ncbi request reprint Subplasmalemmal mitochondria modulate the activity of plasma membrane Ca2+-ATPases
    Maud Frieden
    Department of Cell Physiology and Metabolism, University of Geneva Medical School, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    J Biol Chem 280:43198-208. 2005
    ..Cells thus adapt to the lack of subplasmalemmal mitochondria by relying on external rather than on internal Ca2+ for signaling...
  9. doi request reprint SLP-2 negatively modulates mitochondrial sodium-calcium exchange
    Sandrine Da Cruz
    Department of Cell Biology, University of Geneva, Switzerland
    Cell Calcium 47:11-8. 2010
    ..These data show that SLP-2 modulates mitochondrial calcium extrusion, thereby altering the ability of mitochondria to buffer Ca(2+) and to shape cytosolic Ca(2+) signals...
  10. doi request reprint Do Hv1 proton channels regulate the ionic and redox homeostasis of phagosomes?
    Antoun El Chemaly
    Department of Cell Physiology and Metabolism, University of Geneva, 1211 Geneva 4, Switzerland
    Mol Cell Endocrinol 353:82-7. 2012
    ....
  11. pmc Selective actions of mitochondrial fission/fusion genes on metabolism-secretion coupling in insulin-releasing cells
    Kyu Sang Park
    Department of Cell Physiology and Metabolism, University of Geneva, CH 1211, Geneva 4, Switzerland
    J Biol Chem 283:33347-56. 2008
    ..Through their impact on mitochondrial bioenergetics and distribution, hFis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis...
  12. ncbi request reprint NOX5 is expressed at the plasma membrane and generates superoxide in response to protein kinase C activation
    Lena Serrander
    Foundation for Medical Research, University of Geneva, 64 av de la Roseraie, 1205 Geneva, Switzerland
    Biochimie 89:1159-67. 2007
    ..In summary, we demonstrate that NOX5 can be found intracellularly and at the cell surface. We also describe that it can be activated through protein kinase C, in addition to its Ca(2+) activation...
  13. doi request reprint Morphological and functional aspects of STIM1-dependent assembly and disassembly of store-operated calcium entry complexes
    Wei Wei Shen
    Department of Cell Physiology and Metabolism, University of Geneva, Rue Michel Servet 1, CH 1211 Geneva, Switzerland
    Biochem Soc Trans 40:112-8. 2012
    ..The thin cortical ER consists of thinner sections of the cortical ER enriched in STIM1 and devoid of chaperones that appear to be specialized ER compartments dedicated to Ca2+ signalling...
  14. doi request reprint OPA1 promotes pH flashes that spread between contiguous mitochondria without matrix protein exchange
    Jaime Santo-Domingo
    Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland
    EMBO J 32:1927-40. 2013
    ....
  15. doi request reprint STIM1 juxtaposes ER to phagosomes, generating Ca²⁺ hotspots that boost phagocytosis
    Paula Nunes
    Department of Cell Physiology and Metabolism, University of Geneva, 1211 Geneva 4, Switzerland
    Curr Biol 22:1990-7. 2012
    ..Here, we show that the ER Ca(2+) sensor stromal interaction molecule 1 (STIM1) sustains high-efficiency phagocytosis by recruiting thin ER cisternae that interact productively but do not fuse with phagosomes...
  16. pmc Matrix alkalinization: a novel mitochondrial signal for sustained pancreatic beta-cell activation
    Andreas Wiederkehr
    Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland
    EMBO J 28:417-28. 2009
    ..We propose that the mitochondrial pH and DeltapH are key determinants of mitochondrial energy metabolism and metabolite transport important for cell activation...
  17. pmc Uncoupling protein 3 (UCP3) modulates the activity of Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) by decreasing mitochondrial ATP production
    Umberto De Marchi
    Department of Cell Physiology and Metabolism, University of Geneva, rue Michel Servet, 1, CH 1211 Geneve, Switzerland
    J Biol Chem 286:32533-41. 2011
    ..The sensitivity of SERCA to mitochondrial ATP production suggests that mitochondria control the local ATP availability at ER Ca(2+) uptake and release sites...
  18. pmc Mitochondrial Ca2+ uptake from plasma membrane Cav3.2 protein channels contributes to ischemic toxicity in PC12 cells
    Yves Gouriou
    Department of Cell Physiology and Metabolism, University of Geneva, Geneva CH 1211, Switzerland
    J Biol Chem 288:12459-68. 2013
    ..1/Cav3.2 silencing. We conclude that the opening of Cav3.2 channels during ischemia contribute to the entry of Ca(2+) ions that are transmitted to mitochondria, resulting in a deleterious mitochondrial Ca(2+) overload...
  19. pmc Local cytosolic Ca2+ elevations are required for stromal interaction molecule 1 (STIM1) de-oligomerization and termination of store-operated Ca2+ entry
    Wei Wei Shen
    Department of Cell Physiology and Metabolism, University of Geneva, Rue Michel Servet 1, CH 1211 Geneva 4, Switzerland
    J Biol Chem 286:36448-59. 2011
    ..These data indicate that the refilling of ER Ca(2+) stores is not sufficient to induce STIM1 de-oligomerization and that localized Ca(2+) elevations in the vicinity of assembled SOCE complexes are required for the termination of SOCE...
  20. doi request reprint Remodelling of the endoplasmic reticulum during store-operated calcium entry
    Wei Wei Shen
    Department of Cell Physiology and Metabolism, University of Geneva, 1 rue Michel Servet CH 1211 Geneva 4, Switzerland
    Biol Cell 103:365-80. 2011
    ..Here, we review the molecular interactions and the morphological changes in ER structure that occur during the SOCE process...
  21. pmc Dynamic regulation of the mitochondrial proton gradient during cytosolic calcium elevations
    Damon Poburko
    Department of Cell Physiology and Metabolism, University of Geneva Medical School, Geneva, Switzerland
    J Biol Chem 286:11672-84. 2011
    ..These findings indicate that the mitochondrial matrix pH and ΔpH(m) are regulated by opposing Ca(2+)-dependent processes of stimulated mitochondrial respiration and cytosolic acidification...
  22. doi request reprint Mitochondrial regulation of sarcoplasmic reticulum Ca2+ content in vascular smooth muscle cells
    Damon Poburko
    Department of Cell Physiology and Metabolism, University of Geneva, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    Circ Res 104:104-12. 2009
    ....
  23. pmc From the Cover: STIM1-induced precortical and cortical subdomains of the endoplasmic reticulum
    Lelio Orci
    Department of Cell Physiology and Metabolism, University of Geneva Medical School, 1211 Geneva 4, Switzerland
    Proc Natl Acad Sci U S A 106:19358-62. 2009
    ..These observations form the basis for future structure-function analysis of cortical ER...
  24. doi request reprint STIM1- and Orai1-dependent store-operated calcium entry regulates human myoblast differentiation
    Basile Darbellay
    Department of Clinical Neurosciences, Faculty of Medicine, University of Geneva, Switzerland
    J Biol Chem 284:5370-80. 2009
    ..These findings indicate that STIM1 and Orai1 are key molecules for the induction of human myoblast differentiation...
  25. ncbi request reprint Regulation of the NADPH oxidase and associated ion fluxes during phagocytosis
    Paula Nunes
    Department of Cell Physiology and Metabolism, University of Geneva, 1 rue Michel Servet, CH 1211, Geneva 4, Switzerland
    Traffic 14:1118-31. 2013
    ..Finally, periphagosomal calcium microdomains and calcium-dependent S100A8/9 protein recruitment may help fine-tune spatiotemporal regulation of NADPH oxidase activation for an effective immune response...
  26. pmc VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification
    Antoun El Chemaly
    Department of Cell Physiology and Metabolism, University of Geneva, 1211 Geneva 4, Switzerland
    J Exp Med 207:129-39. 2010
    ..Loss of proton channels thus aborts superoxide production and causes a severe signaling defect in neutrophils...
  27. ncbi request reprint STIM1 knockdown reveals that store-operated Ca2+ channels located close to sarco/endoplasmic Ca2+ ATPases (SERCA) pumps silently refill the endoplasmic reticulum
    Hélène Jousset
    Department of Cell Physiology and Metabolism, University of Geneva Medical School, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    J Biol Chem 282:11456-64. 2007
    ..These findings are consistent with the formation of microdomains containing not only SOC channels on the plasma membrane and STIM proteins on the ER but also SERCA pumps and mitochondria to refill the ER without perturbing the cytosol...
  28. ncbi request reprint Calcium uptake mechanisms of mitochondria
    Jaime Santo-Domingo
    Department of Cell Physiology and Metabolism, University of Geneva, 1, rue Michel Servet, CH 1211 Geneva 4, Switzerland
    Biochim Biophys Acta 1797:907-12. 2010
    ..In this article, we will review the properties of the Ca2+ import systems of mitochondria and discuss how Ca2+ uptake via an electrogenic 1:1 Ca2+/H+ antiport challenges our current thinking of the mitochondrial Ca2+ uptake mechanism...
  29. ncbi request reprint Calreticulin differentially modulates calcium uptake and release in the endoplasmic reticulum and mitochondria
    Serge Arnaudeau
    Department of Physiology, University of Geneva, 1211 Geneva 4, Switzerland
    J Biol Chem 277:46696-705. 2002
    ..The increased Ca(2+) turnover between the two organelles might damage mitochondria, accounting for the increased susceptibility of cells expressing high levels of calreticulin to apoptotic stimuli...
  30. pmc Endosome-to-cytosol transport of viral nucleocapsids
    Isabelle Le Blanc
    Biochemistry Department, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    Nat Cell Biol 7:653-64. 2005
    ....
  31. ncbi request reprint HIV-1 trafficking to the dendritic cell-T-cell infectious synapse uses a pathway of tetraspanin sorting to the immunological synapse
    Eduardo Garcia
    Department of Dermatology and Venereology, University Hospital of Geneva, Geneva, Switzerland
    Traffic 6:488-501. 2005
    ....
  32. pmc Voltage- and NADPH-dependence of electron currents generated by the phagocytic NADPH oxidase
    Gábor L Petheo
    Department of Cell Physiology and Metabolism, University of Geneva Medical School, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    Biochem J 388:485-91. 2005
    ..The surprising voltage-independence of I(e) reported in whole-cell perforated patch recordings was most likely due to substrate limitation and is not an intrinsic property of the oxidase...
  33. doi request reprint The potential role of cobalt ions released from metal prosthesis on the inhibition of Hv1 proton channels and the decrease in Staphyloccocus epidermidis killing by human neutrophils
    Samira Daou
    Department of Cell Physiology and Metabolism, University of Geneva, 1 rue Michel Servet CH 1211 Geneva 4, Switzerland
    Biomaterials 32:1769-77. 2011
    ..By inhibiting proton channels, the Co(2+) ions released by metal prostheses might therefore promote bacterial infections in patients with metal-on-metal total hip arthroplasty...
  34. doi request reprint Regulation of plasma membrane calcium fluxes by mitochondria
    Nicolas Demaurex
    Department of Cell Physiology and Metabolism, University of Geneva, 1, rue Michel Servet, CH 1211 Geneva 4, Switzerland
    Biochim Biophys Acta 1787:1383-94. 2009
    ..In this chapter, we will review the ionic basis of calcium handling by mitochondria and discuss the mechanisms that these organelles use to regulate the activity of plasma membrane calcium channels and transporters...
  35. pmc Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase
    Gábor L Petheo
    Department of Cell Physiology and Metabolism, University of Geneva Medical School, 1 Michel Servet, CH 1211 Geneva 4, Switzerland
    Biochem J 400:431-8. 2006
    ....
  36. doi request reprint Mitochondrial calcium handling during ischemia-induced cell death in neurons
    Yves Gouriou
    Department of Cell Physiology and Metabolism, University of Geneva, Rue Michel Servet 1, Geneve, Switzerland
    Biochimie 93:2060-7. 2011
    ....
  37. pmc NOX family NADPH oxidases: do they have built-in proton channels?
    Andres Maturana
    Department of Physiology, University of Geneva, 1211 Geneva, Switzerland
    J Gen Physiol 120:781-6. 2002
  38. ncbi request reprint ARF1 regulates Nef-induced CD4 degradation
    Julien Faure
    Department of Biochemistry, University of Geneva, 1211 Geneva, Switzerland
    Curr Biol 14:1056-64. 2004
    ..The HIV Nef protein downregulates CD4 through sequential connection with clathrin-coated pits and the COP1 coatomer, resulting in accelerated endocytosis and lysosomal targeting...
  39. ncbi request reprint Measurement of intracellular Ca2+ concentration
    Nicolas Demaurex
    Department of Physiology, University of Geneva Medical Center, CH 1211, Geneva 4, Switzerland
    Methods Cell Biol 70:453-74. 2002
  40. ncbi request reprint Spontaneous calcium oscillations control c-fos transcription via the serum response element in neuroendocrine cells
    Andres Maturana
    Fondation pour Recherches Medicales, University of Geneva, 1211 Geneva 4, Switzerland
    J Biol Chem 277:39713-21. 2002
    ..Further induction of c-fos expression by depolarization requires a nuclear Ca2+ increase...
  41. pmc Physiological roles of voltage-gated proton channels in leukocytes
    Nicolas Demaurex
    Department of Cell Physiology and Metabolism, University of Geneva, CH 1211 Geneva 4, Switzerland
    J Physiol 588:4659-65. 2010
    ..Proton channels are therefore multitalented channels that control male fertility as well as our innate and adaptive immunity...
  42. doi request reprint The role of calcium signaling in phagocytosis
    Paula Nunes
    Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland
    J Leukoc Biol 88:57-68. 2010
    ..Understanding the molecular basis of the Ca(2+) signals that control phagocytosis might provide new, therapeutic tools against pathogens that subvert phagocytic killing...
  43. doi request reprint Local and global calcium signals associated with the opening of neuronal alpha7 nicotinic acetylcholine receptors
    Dorothée Gilbert
    Bioimaging Core Facility, University of Geneva, Switzerland
    Cell Calcium 45:198-207. 2009
    ..Deciphering the rich pattern of intracellular Ca(2+) signals generated by the activity of the alpha7-nAChRs will reveal the physiological role of these receptor-channels...
  44. ncbi request reprint pH Homeostasis of cellular organelles
    Nicolas Demaurex
    Department of Physiology, University of Geneva, 1211 Geneva 4, Switzerland
    News Physiol Sci 17:1-5. 2002
    ....
  45. pmc Subplasmalemmal endoplasmic reticulum controls K(Ca) channel activity upon stimulation with a moderate histamine concentration in a human umbilical vein endothelial cell line
    Maud Frieden
    Department of Medical Biochemistry and Medical Molecular Biology, Karl Franzens University of Graz, Harrachgasse 21 III, A 8010 Graz, Austria
    J Physiol 540:73-84. 2002
    ..These findings further support our concept of the subplasmalemmal Ca(2+) control unit (SCCU) and add the local activation of Ca(2+)-activated K(+)-channels to the function of the SCCU...
  46. ncbi request reprint Sustained Ca2+ transfer across mitochondria is Essential for mitochondrial Ca2+ buffering, sore-operated Ca2+ entry, and Ca2+ store refilling
    Roland Malli
    Department of Medical Biochemistry and Medical Molecular Biology, University of Graz, 8010 Graz, Austria
    J Biol Chem 278:44769-79. 2003
    ....
  47. ncbi request reprint Intracellular transport of calcium from plasma membrane to mitochondria in adrenal H295R cells: implication for steroidogenesis
    Nathalie Lalevee
    Division of Endocrinology and Diabetology, Department of Internal Medicine, University Hospital, CH 1211 Geneva 14, Switzerland
    Endocrinology 144:4575-85. 2003
    ..The response to potassium is proposed to be dependent on the endoplasmic reticulum-mediated transfer of calcium entering through plasma membrane calcium channels to the mitochondria...
  48. ncbi request reprint Endoplasmic reticulum Ca2+ measurements reveal that the cardiac ryanodine receptor mutations linked to cardiac arrhythmia and sudden death alter the threshold for store-overload-induced Ca2+ release
    Peter P Jones
    Department of Physiology and Biophysics, University of Calgary, Calgary, AB, Canada
    Biochem J 412:171-8. 2008
    ..We conclude that the cytosolic loop between TM 6 and TM 7a plays an important role in determining the SOICR threshold and that the alteration of the threshold for SOICR is a common mechanism for RyR2-associated ventricular arrhythmia...
  49. ncbi request reprint A tyrosine-based sorting signal is involved in connexin43 stability and gap junction turnover
    Marc A Thomas
    Institute of Pharmacology and Toxicology, University of Lausanne, 1005 Lausanne, Switzerland
    J Cell Sci 116:2213-22. 2003
    ....
  50. ncbi request reprint Molecular and functional characterization of a new X-linked chronic granulomatous disease variant (X91+) case with a double missense mutation in the cytosolic gp91phox C-terminal tail
    Marie José Stasia
    GREPI EA 2938 UJF, Laboratoire d Enzymologie, CHU 38043 Grenoble Cedex 9, France
    Biochim Biophys Acta 1586:316-30. 2002
    ..We concluded that residues 303 and 304 are crucial for the stable assembly of the NADPH oxidase complex and for electron transfer, but not for its proton channel activity...
  51. pmc Gelsolin mediates calcium-dependent disassembly of Listeria actin tails
    Laura Larson
    Department of Medicine, Division of Infectious Diseases, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:1921-6. 2005
    ..These experiments illustrate the unique and complementary functions of gelsolin and ADF/cofilin in the recycling of actin filaments...