Didier Trono

Summary

Country: Switzerland

Publications

  1. pmc Gene therapy: too much splice can spoil the dish
    Didier Trono
    Frontiers in Genetics Program and School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Clin Invest 122:1600-2. 2012
  2. pmc The integrase interactor 1 (INI1) proteins facilitate Tat-mediated human immunodeficiency virus type 1 transcription
    Yasuo Ariumi
    Department of Microbiology and Molecular Medicine, University of Geneva and Frontiers in Genetics National Center for Competence in Research, Switzerland
    Retrovirology 3:47. 2006
  3. doi request reprint HIV persistence and the prospect of long-term drug-free remissions for HIV-infected individuals
    Didier Trono
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Science 329:174-80. 2010
  4. doi request reprint De novo DNA methylation of endogenous retroviruses is shaped by KRAB-ZFPs/KAP1 and ESET
    Helen M Rowe
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    Development 140:519-29. 2013
  5. pmc The KRAB-ZFP/KAP1 system contributes to the early embryonic establishment of site-specific DNA methylation patterns maintained during development
    Simon Quenneville
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Cell Rep 2:766-73. 2012
  6. doi request reprint KAP1 regulates gene networks controlling T-cell development and responsiveness
    Francesca R Santoni de Sio
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    FASEB J 26:4561-75. 2012
  7. pmc A gene-rich, transcriptionally active environment and the pre-deposition of repressive marks are predictive of susceptibility to KRAB/KAP1-mediated silencing
    Sylvain Meylan
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    BMC Genomics 12:378. 2011
  8. pmc KAP1 regulates gene networks controlling mouse B-lymphoid cell differentiation and function
    Francesca R Santoni de Sio
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Blood 119:4675-85. 2012
  9. pmc A KRAB/KAP1-miRNA cascade regulates erythropoiesis through stage-specific control of mitophagy
    Isabelle Barde
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Science 340:350-3. 2013
  10. doi request reprint Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma
    Karolina Bojkowska
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Hepatology 56:1279-90. 2012

Collaborators

Detail Information

Publications40

  1. pmc Gene therapy: too much splice can spoil the dish
    Didier Trono
    Frontiers in Genetics Program and School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Clin Invest 122:1600-2. 2012
    ..The finding that this is a general phenomenon is concerning, but some of their results do suggest approaches for the development of safeguards in gene therapy vector design...
  2. pmc The integrase interactor 1 (INI1) proteins facilitate Tat-mediated human immunodeficiency virus type 1 transcription
    Yasuo Ariumi
    Department of Microbiology and Molecular Medicine, University of Geneva and Frontiers in Genetics National Center for Competence in Research, Switzerland
    Retrovirology 3:47. 2006
    ..These results suggest that the incoming PICs might recruit INI1 to facilitate proviral transcription...
  3. doi request reprint HIV persistence and the prospect of long-term drug-free remissions for HIV-infected individuals
    Didier Trono
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Science 329:174-80. 2010
    ....
  4. doi request reprint De novo DNA methylation of endogenous retroviruses is shaped by KRAB-ZFPs/KAP1 and ESET
    Helen M Rowe
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    Development 140:519-29. 2013
    ..KRAB-ZFPs, KAP1 and ESET are thus likely to be responsible for the early embryonic instatement of stable epigenetic marks at ERV-containing loci...
  5. pmc The KRAB-ZFP/KAP1 system contributes to the early embryonic establishment of site-specific DNA methylation patterns maintained during development
    Simon Quenneville
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Cell Rep 2:766-73. 2012
    ..Therefore, KRAB-ZFPs and their cofactor KAP1 are in part responsible for the establishment during early embryogenesis of site-specific DNA methylation patterns that are maintained through development...
  6. doi request reprint KAP1 regulates gene networks controlling T-cell development and responsiveness
    Francesca R Santoni de Sio
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    FASEB J 26:4561-75. 2012
    ..These results reveal the so far unsuspected yet important role of KAP1-mediated epigenetic regulation in T-lymphocyte differentiation and activation...
  7. pmc A gene-rich, transcriptionally active environment and the pre-deposition of repressive marks are predictive of susceptibility to KRAB/KAP1-mediated silencing
    Sylvain Meylan
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    BMC Genomics 12:378. 2011
    ..We have recently shown that KRAB/KAP1 can mediate long-range transcriptional repression through heterochromatin spreading, but also demonstrated that this process is at times countered by endogenous influences...
  8. pmc KAP1 regulates gene networks controlling mouse B-lymphoid cell differentiation and function
    Francesca R Santoni de Sio
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Blood 119:4675-85. 2012
    ..Our results therefore reveal the role of KRAB/KAP1-mediated epigenetic regulation in B-cell development and homeostasis...
  9. pmc A KRAB/KAP1-miRNA cascade regulates erythropoiesis through stage-specific control of mitophagy
    Isabelle Barde
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Science 340:350-3. 2013
    ..Thus, a multilayered transcription regulatory system is present, in which protein- and RNA-based repressors are superimposed in combinatorial fashion to govern the timely triggering of an important differentiation event...
  10. doi request reprint Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma
    Karolina Bojkowska
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Hepatology 56:1279-90. 2012
    ....
  11. pmc In embryonic stem cells, ZFP57/KAP1 recognize a methylated hexanucleotide to affect chromatin and DNA methylation of imprinting control regions
    Simon Quenneville
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Mol Cell 44:361-72. 2011
    ..These results significantly advance our understanding of imprinting and suggest a general mechanism for the protection of specific loci against the wave of DNA demethylation that affects the mammalian genome during early embryogenesis...
  12. pmc Structure-function analyses point to a polynucleotide-accommodating groove essential for APOBEC3A restriction activities
    Yannick Bulliard
    School of Life Sciences and Frontiers in Genetics National Program, Ecole Polytechnique Fe de rale de Lausanne, 1015 Lausanne, Switzerland
    J Virol 85:1765-76. 2011
    ..These results suggest that the target specificity of APOBEC3 family members is partly defined by structural features influencing their interaction with polynucleotide substrates...
  13. pmc The Krüppel-associated box repressor domain can induce reversible heterochromatization of a mouse locus in vivo
    Anna C Groner
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    J Biol Chem 287:25361-9. 2012
    ..This finding not only sheds light on KRAB-mediated transcriptional processes, but also suggests approaches for the externally controllable and reversible modulation of chromatin and transcription in vivo...
  14. pmc Global and stage specific patterns of Krüppel-associated-box zinc finger protein gene expression in murine early embryonic cells
    Andrea Corsinotti
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    PLoS ONE 8:e56721. 2013
    ..These results pave the way to delineating the role of specific KRAB-ZFPs in early embryogenesis...
  15. pmc KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading
    Anna C Groner
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    PLoS Genet 6:e1000869. 2010
    ..This finding not only suggests auto-regulatory mechanisms in the control of KRAB-ZFP gene clusters, but also provides important cues for interpreting future genome-wide DNA binding data of KRAB-ZFPs and KAP1...
  16. doi request reprint Inducible gene and shRNA expression in resident hematopoietic stem cells in vivo
    Elisa Laurenti
    Ecole Polytechnique Federale de Lausanne EPFL, ISREC Swiss Institute for Experimental Cancer Research, School of Life Science, and Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Stem Cells 28:1390-8. 2010
    ..In summary, this easy to implement conditional system provides inducible and reversible overexpression or knock down of genes in resident HSCs in vivo using a drug devoid of toxic or activating effects...
  17. doi request reprint KAP1 controls endogenous retroviruses in embryonic stem cells
    Helen M Rowe
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Nature 463:237-40. 2010
    ..Correspondingly, IAP 5'UTR sequences can impose in cis KAP1-dependent repression on a heterologous promoter in ES cells. Our results establish that KAP1 controls endogenous retroelements during early embryonic development...
  18. pmc Functional analysis and structural modeling of human APOBEC3G reveal the role of evolutionarily conserved elements in the inhibition of human immunodeficiency virus type 1 infection and Alu transposition
    Yannick Bulliard
    School of Life Sciences and Frontiers in Genetics National Center of Competence in Research, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    J Virol 83:12611-21. 2009
    ....
  19. pmc APOBEC3-independent interferon-induced viral clearance in hepatitis B virus transgenic mice
    Priscilla Turelli
    Global Health Institute, School of Life Sciences and Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    J Virol 82:6585-90. 2008
    ..We conclude that APOBEC3 is not an essential mediator of the IFN-mediated inhibition of HBV in vivo...
  20. pmc Homology-based identification of capsid determinants that protect HIV1 from human TRIM5α restriction
    Pierre V Maillard
    Global Health Institute, School of Life Sciences, and Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne, Switzerland
    J Biol Chem 286:8128-40. 2011
    ..These results indicate that retroviruses have evolved similar mechanisms to escape TRIM5α restriction via the interference of structurally homologous determinants in the viral capsid...
  21. pmc TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells
    Helen M Rowe
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Genome Res 23:452-61. 2013
    ..TRIM28-mediated control of ERVs is therefore crucial not just to prevent retrotransposition, but more broadly to safeguard the transcriptional dynamics of early embryos...
  22. pmc The specificity of TRIM5 alpha-mediated restriction is influenced by its coiled-coil domain
    Pierre V Maillard
    Global Health Institute, School of Life Sciences, and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Virol 84:5790-801. 2010
    ..These results indicate that the PRYSPRY and coiled-coil domains cooperate to determine the specificity of TRIM5 alpha-mediated capture of retroviral capsids, shedding new light on this complex event...
  23. ncbi request reprint The Kruppel-associated box repressor domain can trigger de novo promoter methylation during mouse early embryogenesis
    Maciej Wiznerowicz
    School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Biol Chem 282:34535-41. 2007
    ..Here we reveal that, in sharp contrast, KRAB-mediated repression results in irreversible gene silencing through promoter DNA methylation if it acts during the first few days of mouse development...
  24. pmc Interfering residues narrow the spectrum of MLV restriction by human TRIM5alpha
    Pierre V Maillard
    Global Health Institute, School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    PLoS Pathog 3:e200. 2007
    ....
  25. ncbi request reprint Profaning the Ultimate Sanctuary: HIV Latency in Hematopoietic Stem Cells
    Didier Trono
    School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Cell Host Microbe 9:170-2. 2011
    ..That latent proviruses might also hide in multipotent hematopoietic stem cells suggests an even more formidable challenge and potentially has therapeutic implications...
  26. doi request reprint Measuring in vivo protein half-life
    Karolina Bojkowska
    School of Life Sciences, Frontiers in Genetics National Program, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 18:805-15. 2011
    ..These results open broad perspectives for studying protein function in living animals...
  27. pmc Characterization of APOBEC3G binding to 7SL RNA
    Daniel Bach
    Global Health Institute, School of Life Sciences and Frontiers in Genetics, National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Retrovirology 5:54. 2008
    ..We thus conclude that 7SL RNA is not an essential mediator of the virion packaging of these antiviral cytidine deaminases...
  28. doi request reprint MicroRNA-124 is a subventricular zone neuronal fate determinant
    Malin Akerblom
    Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, 22184 Lund, Sweden
    J Neurosci 32:8879-89. 2012
    ..In summary, our results demonstrate that miR-124 is a neuronal fate determinant in the subventricular zone...
  29. doi request reprint Production and titration of lentiviral vectors
    Isabelle Barde
    School of Life Sciences, École Polytechnique Fédérale de Lausanne and Frontiers in Genetics, National Center of Competence in Research, Lausanne, Switzerland
    Curr Protoc Neurosci . 2010
    ..This unit also describes protocols for production and titration of LVs that can be implemented in a research laboratory setting, with an emphasis on standardization to improve transposability of results between laboratories...
  30. doi request reprint Dynamic control of endogenous retroviruses during development
    Helen M Rowe
    National Program, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Virology 411:273-87. 2011
    ....
  31. doi request reprint KAP1-mediated epigenetic repression in the forebrain modulates behavioral vulnerability to stress
    Johan Jakobsson
    School of Life Sciences, National Center of Competence in Research, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Neuron 60:818-31. 2008
    ..We propose a model in which the tethering of KAP1-associated chromatin remodeling factors via KRAB-ZFPs epigenetically controls gene expression in the hippocampus, thereby conditioning responses to behavioral stress...
  32. pmc Induction of antiviral cytidine deaminases does not explain the inhibition of hepatitis B virus replication by interferons
    Stephanie Jost
    School of Life Sciences and Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Virol 81:10588-96. 2007
    ..We conclude that these cytidine deaminases are not essential effectors of IFN in its action against this pathogen...
  33. doi request reprint A switch between topological domains underlies HoxD genes collinearity in mouse limbs
    Guillaume Andrey
    School of Life Sciences, Federal Institute of Technology, Lausanne, 1015 Lausanne, Switzerland
    Science 340:1234167. 2013
    ..This regulatory strategy accounts for collinear Hox gene regulation in land vertebrate appendages...
  34. pmc APOBEC3G-depleted resting CD4+ T cells remain refractory to HIV1 infection
    Francesca R Santoni de Sio
    Global Health Institute, School of Life Sciences, Frontiers in Genetics National Center of Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    PLoS ONE 4:e6571. 2009
    ..Chiu et al. recently proposed that this restriction reflects the action of APOBEC3G (A3G). They further suggested that T cell activation abrogates the A3G-mediated block by directing this protein to a high molecular mass complex...
  35. pmc Regulation of episomal gene expression by KRAB/KAP1-mediated histone modifications
    Isabelle Barde
    School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Virol 83:5574-80. 2009
    ....
  36. pmc Genotypic features of lentivirus transgenic mice
    Marc Olivier Sauvain
    Ecole Polytechnique Federale de Lausanne, School of Life Sciences, Lausanne CH 1015, Switzerland
    J Virol 82:7111-9. 2008
    ....
  37. pmc Model structure of human APOBEC3G
    Kun Lin Zhang
    Institute of Microbiology, University Hospital Center, University of Lausanne, Lausanne, Switzerland
    PLoS ONE 2:e378. 2007
    ..There is no 3-D structure of APOBEC3G solved by X-ray or nuclear magnetic resonance...
  38. ncbi request reprint KRAB can repress lentivirus proviral transcription independently of integration site
    Yannick Bulliard
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    J Biol Chem 281:35742-6. 2006
    ..These results indicate that KRAB-induced transcriptional repression is robust and active over a variety of genomic contexts that include at least the wide range of sites targeted by lentiviral integration...
  39. doi request reprint Innate immunity against retroviral pathogens: from an ambiguous genetic self to novel therapeutic approaches
    Sylvain Meylan
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    Swiss Med Wkly 139:706-11. 2009
    ..Their presence in higher species thus reflects a delicate equilibrium between occasional permissiveness and tight restriction, through innate immunity mechanisms also engaged in protection against their exogenous viral counterparts...
  40. ncbi request reprint Editing at the crossroad of innate and adaptive immunity
    Priscilla Turelli
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Science 307:1061-5. 2005
    ..Exciting questions are raised, which take us to the depth of the intimate relations between vertebrates and the microbial underworld...