Manfred Zinn

Summary

Affiliation: Swiss Federal Laboratories for Materials Testing and Research
Country: Switzerland

Publications

  1. ncbi request reprint The concept of multiple-nutrient-limited growth of microorganisms and its application in biotechnological processes
    Thomas Egli
    Department of Microbiology, Swiss Federal Institute for Environmental Science and Technology EAWAG, P O Box 611, Uberlandstrasse 133, CH 8600 Dubendorf, Switzerland
    Biotechnol Adv 22:35-43. 2003
  2. ncbi request reprint Bacterial poly(hydroxyalkanoates) as a source of chiral hydroxyalkanoic acids
    Qun Ren
    Biocompatible Materials, Materials Science and Technology Empa, 9014 St Gallen, Switzerland
    Biomacromolecules 6:2290-8. 2005
  3. ncbi request reprint Quantitative analysis of bacterial medium-chain-length poly([R]-3-hydroxyalkanoates) by gas chromatography
    Patrick Furrer
    Empa, Materials Science and Technology, Laboratory for Biomaterials, Lerchenfeldstr 5, CH 9014 St Gallen, Switzerland
    J Chromatogr A 1143:199-206. 2007
  4. ncbi request reprint Autoxidation of medium chain length polyhydroxyalkanoate
    Manfred Schmid
    Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, Lerchenfeldstrasse 5, CH 9014 St Gallen, Switzerland
    Biomacromolecules 8:579-84. 2007
  5. pmc Influence of growth stage on activities of polyhydroxyalkanoate (PHA) polymerase and PHA depolymerase in Pseudomonas putida U
    Qun Ren
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Science and Technology Empa, CH 9014 St, Gallen, Switzerland
    BMC Microbiol 10:254. 2010
  6. ncbi request reprint Dual nutrient limited growth: models, experimental observations, and applications
    Manfred Zinn
    Biocompatible Materials, Swiss Laboratories for Materials Testing and Research EMPA, Lerchenfeldstrasse 5, CH 9014 St Gallen
    J Biotechnol 113:263-79. 2004
  7. doi request reprint Growth and accumulation dynamics of poly(3-hydroxyalkanoate) (PHA) in Pseudomonas putida GPo1 cultivated in continuous culture under transient feed conditions
    Manfred Zinn
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Science and Technology Empa, St Gallen, Switzerland
    Biotechnol J 6:1240-52. 2011
  8. pmc Overexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1
    Qun Ren
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Testing and Research Empa, CH 9014 St, Gallen, Switzerland
    Microb Cell Fact 8:60. 2009
  9. doi request reprint Simultaneous accumulation and degradation of polyhydroxyalkanoates: futile cycle or clever regulation?
    Qun Ren
    Laboratory of Biomaterials, Swiss Federal Laboratories for Materials Testing and Research Empa, CH 9014 St Gallen, Switzerland
    Biomacromolecules 10:916-22. 2009
  10. ncbi request reprint Poly(3-hydroxyalkanoate) polymerase synthesis and in vitro activity in recombinant Escherichia coli and Pseudomonas putida
    Qun Ren
    Biocompatible Materials, Materials Science and Technology Empa, 9014 St Gallen, Switzerland
    Appl Microbiol Biotechnol 69:286-92. 2005

Collaborators

Detail Information

Publications26

  1. ncbi request reprint The concept of multiple-nutrient-limited growth of microorganisms and its application in biotechnological processes
    Thomas Egli
    Department of Microbiology, Swiss Federal Institute for Environmental Science and Technology EAWAG, P O Box 611, Uberlandstrasse 133, CH 8600 Dubendorf, Switzerland
    Biotechnol Adv 22:35-43. 2003
    ..Specific examples will be given that demonstrate the advantages and the potential of multiple nutrient-limited growth conditions for industrial production processes...
  2. ncbi request reprint Bacterial poly(hydroxyalkanoates) as a source of chiral hydroxyalkanoic acids
    Qun Ren
    Biocompatible Materials, Materials Science and Technology Empa, 9014 St Gallen, Switzerland
    Biomacromolecules 6:2290-8. 2005
    ..We confirmed that the purified 3-hydroxyoctanoic acid monomer has exclusively the R-configuration...
  3. ncbi request reprint Quantitative analysis of bacterial medium-chain-length poly([R]-3-hydroxyalkanoates) by gas chromatography
    Patrick Furrer
    Empa, Materials Science and Technology, Laboratory for Biomaterials, Lerchenfeldstr 5, CH 9014 St Gallen, Switzerland
    J Chromatogr A 1143:199-206. 2007
    ..Boron trifluoride in methanol was successfully applied to quantitatively methanolyse different mcl-PHA (recovery >94%). This novel method is well-suited for the analysis of purified mcl-PHA as well as for mcl-PHA in biomass...
  4. ncbi request reprint Autoxidation of medium chain length polyhydroxyalkanoate
    Manfred Schmid
    Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, Lerchenfeldstrasse 5, CH 9014 St Gallen, Switzerland
    Biomacromolecules 8:579-84. 2007
    ....
  5. pmc Influence of growth stage on activities of polyhydroxyalkanoate (PHA) polymerase and PHA depolymerase in Pseudomonas putida U
    Qun Ren
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Science and Technology Empa, CH 9014 St, Gallen, Switzerland
    BMC Microbiol 10:254. 2010
    ..Another level of control could relate to modulation of the activities of PhaC and PhaZ. In order to investigate the latter, assays for in vitro activity measurements of PhaC and PhaZ in crude cell extracts are necessary...
  6. ncbi request reprint Dual nutrient limited growth: models, experimental observations, and applications
    Manfred Zinn
    Biocompatible Materials, Swiss Laboratories for Materials Testing and Research EMPA, Lerchenfeldstrasse 5, CH 9014 St Gallen
    J Biotechnol 113:263-79. 2004
    ..The aim of this survey is to introduce this special growth feature with respect to basic models, experimental data, and potential applications in bioprocesses...
  7. doi request reprint Growth and accumulation dynamics of poly(3-hydroxyalkanoate) (PHA) in Pseudomonas putida GPo1 cultivated in continuous culture under transient feed conditions
    Manfred Zinn
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Science and Technology Empa, St Gallen, Switzerland
    Biotechnol J 6:1240-52. 2011
    ..3 and 6.9 g/g for gradient 2, and 5.1 and 8.9 g/g for gradient 3. A mathematical equation was developed that compensated a time delay of the gradient that was caused by the wash-in/wash-out effects of the medium feed...
  8. pmc Overexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1
    Qun Ren
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Testing and Research Empa, CH 9014 St, Gallen, Switzerland
    Microb Cell Fact 8:60. 2009
    ..It is problematic to study the intrinsic properties of these enzymes with such low activities, although they are pure...
  9. doi request reprint Simultaneous accumulation and degradation of polyhydroxyalkanoates: futile cycle or clever regulation?
    Qun Ren
    Laboratory of Biomaterials, Swiss Federal Laboratories for Materials Testing and Research Empa, CH 9014 St Gallen, Switzerland
    Biomacromolecules 10:916-22. 2009
    ..These results suggest that PHA metabolism is likely to be controlled by the [acetyl-CoA]/[CoA] and [NADH]/[NAD] ratios. The physiological roles of simultaneous PHA accumulation and degradation are also discussed...
  10. ncbi request reprint Poly(3-hydroxyalkanoate) polymerase synthesis and in vitro activity in recombinant Escherichia coli and Pseudomonas putida
    Qun Ren
    Biocompatible Materials, Materials Science and Technology Empa, 9014 St Gallen, Switzerland
    Appl Microbiol Biotechnol 69:286-92. 2005
    ..Our results suggest that optimal functioning of the PHA polymerase requires factors or a molecular environment that is available in P. putida but not in E. coli...
  11. ncbi request reprint Efficient production of (R)-3-hydroxycarboxylic acids by biotechnological conversion of polyhydroxyalkanoates and their purification
    Katinka Ruth
    Laboratories for Biomaterials, Swiss Federal Laboratories for Materials Testing and Research, Lerchenfeldstrasse 5, CH 9014 St Gallen, Switzerland
    Biomacromolecules 8:279-86. 2007
    ..All obtained monomers had a purity of over 95 wt %. The physical properties of the purified monomers and their antimicrobial activities were also investigated...
  12. doi request reprint Simultaneous biosynthesis of two copolymers in Pseudomonas putida GPo1 using a two-stage continuous culture system
    Rene Hartmann
    Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, Lerchenfeldstrasse 5, CH 9014 St Gallen, Switzerland
    Biomacromolecules 11:1488-93. 2010
    ..The maximum PHA content was found to be 53.8% of total cell dry weight, which is very high for mcl-PHA...
  13. pmc Enatiomerically pure hydroxycarboxylic acids: current approaches and future perspectives
    Qun Ren
    Swiss Federal Laboratories for Materials Testing and Research Empa, Laboratory for Biomaterials, 9014 St Gallen, Switzerland
    Appl Microbiol Biotechnol 87:41-52. 2010
    ..In this article, through analysis of the current advances in production of these acids, we present guidelines for future developments in biotechnological processes for R-HA production...
  14. ncbi request reprint Efficient recovery of low endotoxin medium-chain-length poly([R]-3-hydroxyalkanoate) from bacterial biomass
    Patrick Furrer
    Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Biomaterials, CH 9014 St Gallen, Switzerland
    J Microbiol Methods 69:206-13. 2007
    ..The polydispersity (M(w)/M(n)) of PHO was decreased from 2.0 to 1.5 for this optimized procedure...
  15. doi request reprint Pressure to kill or pressure to boost: a review on the various effects and applications of hydrostatic pressure in bacterial biotechnology
    Stéphanie Follonier
    Laboratory for Biomaterials, Empa Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St Gallen, Switzerland
    Appl Microbiol Biotechnol 93:1805-15. 2012
    ..This paper gives an overview on the effects of pressures in the range of 1 bar to 10 kbar on bacteria and presents the major and most recent achievements realized in the development of pressure-based biotechnological applications...
  16. doi request reprint Putting cells under pressure: a simple and efficient way to enhance the productivity of medium-chain-length polyhydroxyalkanoate in processes with Pseudomonas putida KT2440
    Stéphanie Follonier
    Laboratory for Biomaterials, Empa Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St Gallen, Switzerland
    Biotechnol Bioeng 109:451-61. 2012
    ..We estimate that the oxygen provided to the culture broth under elevated pressure would be sufficient to triple mcl-PHA productivity in our chemostat system from 3.4 (at 1 bar) to 11 g L(-1)h(-1) (at 3.2 bar)...
  17. pmc Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440
    Sylvaine Le Meur
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Science and Technology Empa, Gallen, Switzerland
    BMC Biotechnol 12:53. 2012
    ..To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered P. putida KT2440 strain...
  18. pmc A reduction in growth rate of Pseudomonas putida KT2442 counteracts productivity advances in medium-chain-length polyhydroxyalkanoate production from gluconate
    Stéphanie Follonier
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Science and Technology Empa, Lerchenfeldstrasse 5, 9000 St, Gallen, Switzerland
    Microb Cell Fact 10:25. 2011
    ....
  19. doi request reprint Biofilm formation by the yeast Rhodotorula mucilaginosa: process, repeatability and cell attachment in a continuous biofilm reactor
    Jasmin Gattlen
    Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials, Lerchenfeldstrasse 5, CH 9014, St Gallen, Switzerland
    Biofouling 27:979-91. 2011
    ..Plasma modification of polypropylene significantly reduced the hydrophobicity but did not enhance cell attachment. The biofilm formed on polypropylene coupons could be used for sanitation studies...
  20. ncbi request reprint Application of activated charcoal in the downstream processing of bacterial olefinic poly(3-hydroxyalkanoates)
    Bruno Wampfler
    Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials, St Gallen
    Chimia (Aarau) 64:784-8. 2010
    ..5 for PHUE and 0.25 for PHOUE. The yields with regard to the raw polymers amounted to 55 wt% for PHUE and 75 wt% for PHOUE, which are acceptable for polymers that can be used for medical applications...
  21. pmc Poly(4-hydroxybutyrate) (P4HB) production in recombinant Escherichia coli: P4HB synthesis is uncoupled with cell growth
    Sylvaine Le Meur
    Laboratory for Biomaterials, Swiss Federal Laboratories for Materials Science and Technology Empa, Lerchenfeldstrasse 5, CH 9014, St, Gallen, Switzerland
    Microb Cell Fact 12:123. 2013
    ....
  22. ncbi request reprint Tailor-made olefinic medium-chain-length poly[(R)-3-hydroxyalkanoates] by Pseudomonas putida GPo1: batch versus chemostat production
    Rene Hartmann
    Materials Science and Technology Empa, Lerchenfeldstrasse 5, CH 9014 St Gallen, Switzerland
    Biotechnol Bioeng 93:737-46. 2006
    ..The fraction of aliphatic monomers in the accumulated mclPHA was slightly lower at high dilution rates and increased towards low dilution rates, again indicating different kinetics for the two carbon substrates in P. putida GPo1...
  23. ncbi request reprint Toward non-toxic antifouling: synthesis of hydroxy-, cinnamic acid-, sulfate-, and zosteric acid-labeled poly[3-hydroxyalkanoates]
    Roland Hany
    Swiss Federal Laboratories for Materials Testing and Research Empa, CH 8600 Dubendorf, Switzerland
    Biomacromolecules 5:1452-6. 2004
    ..These functionalized polyesters are currently investigated as environmentally friendly coatings to protect surfaces from biofouling...
  24. pmc New insights on the reorganization of gene transcription in Pseudomonas putida KT2440 at elevated pressure
    Stéphanie Follonier
    Swiss Federal Laboratories for Materials Science and Technology, Gallen, Switzerland
    Microb Cell Fact 12:30. 2013
    ....
  25. ncbi request reprint Expression of PHA polymerase genes of Pseudomonas putida in Escherichia coli and its effect on PHA formation
    Qun Ren
    Biocompatible Materials, Swiss Federal Laboratories for Materials Testing and Research Empa, Lerchenfeldstrasse 5, 9014 St, Gallen, Switzerland
    Antonie Van Leeuwenhoek 87:91-100. 2005
    ..In addition, the PHA monomer composition differed when different gene expression systems or different phaC genes were applied...
  26. doi request reprint Biofilms isolated from washing machines from three continents and their tolerance to a standard detergent
    Jasmin Gattlen
    Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials, Lerchenfeldstrasse 5, CH 9014 St Gallen, Switzerland
    Biofouling 26:873-82. 2010
    ..However, for both strains the recommended detergent concentration (7 g l(-1)) was insufficient to completely clean surfaces from cell debris and exopolymeric substances...