U Sauer

Summary

Affiliation: Swiss Federal Institute of Technology
Country: Switzerland

Publications

  1. ncbi request reprint Evolutionary engineering of industrially important microbial phenotypes
    U Sauer
    Institute of Biotechnology, ETH Zurich, 8093 Zurich, Switzerland
    Adv Biochem Eng Biotechnol 73:129-69. 2001
  2. pmc Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast
    Lars M Blank
    Institute of Biotechnology, ETH Zurich, 8093 Zurich, Switzerland
    Genome Biol 6:R49. 2005
  3. pmc Model-independent fluxome profiling from 2H and 13C experiments for metabolic variant discrimination
    Nicola Zamboni
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Genome Biol 5:R99. 2004
  4. ncbi request reprint The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Biol Chem 279:6613-9. 2004
  5. ncbi request reprint Inverse metabolic engineering with phosphagen kinase systems improves the cellular energy state
    Uwe Sauer
    Institute of Biotechnology, Swiss Federal Institute of Technology ETH Zurich, CH 8093
    Metab Eng 6:220-8. 2004
  6. ncbi request reprint High-throughput phenomics: experimental methods for mapping fluxomes
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Curr Opin Biotechnol 15:58-63. 2004
  7. pmc Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism
    U Sauer
    Institut fur Biotechnologie, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 181:6679-88. 1999
  8. ncbi request reprint Metabolic capacity of Bacillus subtilis for the production of purine nucleosides, riboflavin, and folic acid
    U Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Bioeng 59:227-38. 1998
  9. ncbi request reprint Metabolic flux analysis with a comprehensive isotopomer model in Bacillus subtilis
    M Dauner
    Institute of Biotechnology, , , Switzerland
    Biotechnol Bioeng 76:144-56. 2001
  10. ncbi request reprint Metabolic fluxes in riboflavin-producing Bacillus subtilis
    U Sauer
    Institut fur Biotechnologie, ETH Zurich, Switzerland
    Nat Biotechnol 15:448-52. 1997

Collaborators

Detail Information

Publications63

  1. ncbi request reprint Evolutionary engineering of industrially important microbial phenotypes
    U Sauer
    Institute of Biotechnology, ETH Zurich, 8093 Zurich, Switzerland
    Adv Biochem Eng Biotechnol 73:129-69. 2001
    ..This review presents evolutionary engineering of whole cells as an emerging methodology that draws on the latest advances from a wide range of scientific and technical disciplines...
  2. pmc Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast
    Lars M Blank
    Institute of Biotechnology, ETH Zurich, 8093 Zurich, Switzerland
    Genome Biol 6:R49. 2005
    ..Here we identify key experiments in a systems biology approach with a genome-scale model of Saccharomyces cerevisiae metabolism, thereby reducing the workload for experimental network analyses and functional genomics...
  3. pmc Model-independent fluxome profiling from 2H and 13C experiments for metabolic variant discrimination
    Nicola Zamboni
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Genome Biol 5:R99. 2004
    ..The approach is flexible and paves the way to large-scale fluxome profiling of any biological system and condition...
  4. ncbi request reprint The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Biol Chem 279:6613-9. 2004
    ..metabolism with an extraordinary flexibility to cope with varying catabolic and anabolic demands, which raises two general questions: why do only a few bacteria contain both isoforms, and how do other organisms manage NADPH metabolism?..
  5. ncbi request reprint Inverse metabolic engineering with phosphagen kinase systems improves the cellular energy state
    Uwe Sauer
    Institute of Biotechnology, Swiss Federal Institute of Technology ETH Zurich, CH 8093
    Metab Eng 6:220-8. 2004
    ..Examples are discussed that illustrate how microbial metabolism can be tailored for large-scale industrial processes with imperfect mixing and how the liver can be protected from metabolic insults or stimulated for better regeneration...
  6. ncbi request reprint High-throughput phenomics: experimental methods for mapping fluxomes
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Curr Opin Biotechnol 15:58-63. 2004
    ..This review highlights recent developments towards high-throughput flux analysis...
  7. pmc Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism
    U Sauer
    Institut fur Biotechnologie, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 181:6679-88. 1999
    ....
  8. ncbi request reprint Metabolic capacity of Bacillus subtilis for the production of purine nucleosides, riboflavin, and folic acid
    U Sauer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Bioeng 59:227-38. 1998
    ..subtilis on glucose were estimated to be 0.343, 0.160, and 0.161 (mol product/mol glucose) for purine nucleosides, riboflavin, and folic acid, respectively. Potential strategies for improving these maximum yields are discussed...
  9. ncbi request reprint Metabolic flux analysis with a comprehensive isotopomer model in Bacillus subtilis
    M Dauner
    Institute of Biotechnology, , , Switzerland
    Biotechnol Bioeng 76:144-56. 2001
    ..Specifically, a chi(2) test was applied to validate the model and the chi-square criterion was used to explore the sensitivity of model predictions to the experimental data...
  10. ncbi request reprint Metabolic fluxes in riboflavin-producing Bacillus subtilis
    U Sauer
    Institut fur Biotechnologie, ETH Zurich, Switzerland
    Nat Biotechnol 15:448-52. 1997
    ..Under the conditions investigated, riboflavin formation in B. subtilis is limited by the fluxes through the biosynthetic rather than the central carbon pathways, which suggests a focus for future metabolic engineering of this system...
  11. ncbi request reprint Stoichiometric growth model for riboflavin-producing Bacillus subtilis
    M Dauner
    Institute of Biotechnology, , , Switzerland
    Biotechnol Bioeng 76:132-43. 2001
    ..These analyses clearly show that verification of macromolecular composition data is important for optimum flux calculations...
  12. pmc Bacillus subtilis metabolism and energetics in carbon-limited and excess-carbon chemostat culture
    M Dauner
    Institute of Biotechnology, , , Switzerland
    J Bacteriol 183:7308-17. 2001
    ..ATP-dependent control of in vivo enzyme activity appears to be irrelevant for the observed differences in TCA cycle fluxes...
  13. ncbi request reprint Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA
    F Canonaco
    Institute of Biotechnology, ETH Zurich, Switzerland
    FEMS Microbiol Lett 204:247-52. 2001
    ..The presented results provide first evidence that UdhA restores the cellular redox balance by catalyzing electron transfer from NADPH to NADH...
  14. ncbi request reprint Overexpression of the ATP-dependent helicase RecG improves resistance to weak organic acids in Escherichia coli
    P Steiner
    Institute of Biotechnology, ETH Zurich, 8093, Zurich, Switzerland
    Appl Microbiol Biotechnol 63:293-9. 2003
    ....
  15. ncbi request reprint Glucose catabolism of Escherichia coli strains with increased activity and altered regulation of key glycolytic enzymes
    M Emmerling
    Institute of Biotechnology, Eidgenossische Technische Hochschule Zurich, Switzerland
    Metab Eng 1:117-27. 1999
    ..The flux data demonstrate that overexpression of even single enzymes early in a central pathway can increase the fluxes to a particular metabolic product, although it may not affect the glucose uptake rate...
  16. pmc Proteins induced during adaptation of Acetobacter aceti to high acetate concentrations
    P Steiner
    Institute of Biotechnology, , Switzerland
    Appl Environ Microbiol 67:5474-81. 2001
    ....
  17. pmc Metabolic networks in motion: 13C-based flux analysis
    Uwe Sauer
    Institute of Molecular Systems Biology, ETH Zurich, Switzerland
    Mol Syst Biol 2:62. 2006
    ....
  18. pmc Computational prediction and experimental verification of the gene encoding the NAD+/NADP+-dependent succinate semialdehyde dehydrogenase in Escherichia coli
    Tobias Fuhrer
    Institute of Molecular Systems Biology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 189:8073-8. 2007
    ..Analysis of available genome sequences demonstrated that orthologs of both yneI and gabD are broadly distributed across phylogenetic space...
  19. ncbi request reprint The PEP-pyruvate-oxaloacetate node as the switch point for carbon flux distribution in bacteria
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, Switzerland
    FEMS Microbiol Rev 29:765-94. 2005
    ..The present knowledge unequivocally reveals the PEP-pyruvate-oxaloacetate nodes of bacteria to be a fascinating target of metabolic engineering in order to achieve optimized metabolite production...
  20. pmc Cyclic AMP-dependent catabolite repression is the dominant control mechanism of metabolic fluxes under glucose limitation in Escherichia coli
    Annik Nanchen
    Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland
    J Bacteriol 190:2323-30. 2008
    ....
  21. pmc Impact of global transcriptional regulation by ArcA, ArcB, Cra, Crp, Cya, Fnr, and Mlc on glucose catabolism in Escherichia coli
    Annik Perrenoud
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 187:3171-9. 2005
    ..coli. This control goes well beyond the previously known ArcA-dependent regulation of the TCA cycle during microaerobiosis...
  22. ncbi request reprint Metabolic-flux and network analysis in fourteen hemiascomycetous yeasts
    Lars M Blank
    Institute of Biotechnology, Building HPT E58, ETH Zurich, 8093 Zurich, Switzerland
    FEMS Yeast Res 5:545-58. 2005
    ..In most yeasts, the PP pathway activity appears to be driven exclusively by the demand for NADPH...
  23. ncbi request reprint Selection of quiescent Escherichia coli with high metabolic activity
    Marco Sonderegger
    Metab Eng 7:4-9. 2005
    ..The metabolically most active clones were identified by screening for high specific glucose uptake rates during ammonium starvation-induced stationary phase in deep-well microtiter plates...
  24. ncbi request reprint Single-gene knockout of a novel regulatory element confers ethionine resistance and elevates methionine production in Corynebacterium glutamicum
    Jörg Mampel
    Institute of Biotechnology, Swiss Federal Institute of Technology ETH, Zurich 8093, Switzerland
    Appl Microbiol Biotechnol 68:228-36. 2005
    ..Complementation of the mutant strain with plasmid-borne NCgl2640 restored the wild-type phenotype for metY and sulfite reductase...
  25. ncbi request reprint Transient expression and flux changes during a shift from high to low riboflavin production in continuous cultures of Bacillus subtilis
    Nicola Zamboni
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Bioeng 89:219-32. 2005
    ..With the possible exception of a slight down-regulation of the purine biosynthesis pathway, genome-wide expression analysis indicated that transcriptional regulation was not responsible for the production decline...
  26. pmc FiatFlux--a software for metabolic flux analysis from 13C-glucose experiments
    Nicola Zamboni
    Institute of Biotechnology, ETH Zurich, 8093 Zurich, Switzerland
    BMC Bioinformatics 6:209. 2005
    ..A conceptual simplification of flux analysis is the analytical determination of metabolic flux ratios exclusively from MS data, which can then be used in a second step to estimate absolute in vivo fluxes...
  27. pmc Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440
    Olga Revelles
    Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda 1, 18008 Granada, Spain
    J Bacteriol 187:7500-10. 2005
    ..This study establishes an unequivocal relationship between gene and pathway enzymes in the metabolism of L-lysine, which is of crucial importance for the successful colonization of the rhizosphere of plants by this microorganism...
  28. ncbi request reprint Determination of metabolic flux ratios from 13C-experiments and gas chromatography-mass spectrometry data: protocol and principles
    Annik Nanchen
    Institute for Molecular Systems Biology, ETH Zurich, Switzerland
    Methods Mol Biol 358:177-97. 2007
    ..These principles are also implemented in the publicly available software FiatFlux, which directly calculates flux ratios from the mass spectra of amino acids...
  29. ncbi request reprint Genetics. Getting closer to the whole picture
    Uwe Sauer
    Institute of Molecular Systems Biology, ETH Zurich, Switzerland
    Science 316:550-1. 2007
  30. ncbi request reprint Experimental tests for an evolutionary trade-off between growth rate and yield in E. coli
    Maja Novak
    Institute of Integrative Biology, Eldgenossische Technische Hochschule Zurich, 8092 Zurich, Switzerland
    Am Nat 168:242-51. 2006
    ..The observation of a trade-off within, but not between, populations suggests that the populations evolved different genetic solutions for growth in the selective environment, which in turn led to different physiological constraints...
  31. pmc YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis
    Guillaume Lerondel
    Microbiologie et Genetique Moleculaire, INRA UMR1238 CNRS UMR2585 and INAP G, F 78850 Thiverval Grignon, France
    J Bacteriol 188:4727-36. 2006
    ..coli partially suppressed it. These results suggest an additional physiological role of YtsJ beyond that of malate-to-pyruvate conversion...
  32. pmc Nonlinear dependency of intracellular fluxes on growth rate in miniaturized continuous cultures of Escherichia coli
    Annik Nanchen
    Institute of Molecular Systems Biology, ETH Zurich, CH 8093 Zurich, Switzerland
    Appl Environ Microbiol 72:1164-72. 2006
    ....
  33. ncbi request reprint Latent pathway activation and increased pathway capacity enable Escherichia coli adaptation to loss of key metabolic enzymes
    Stephen S Fong
    Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond 28284 3028, USA
    J Biol Chem 281:8024-33. 2006
    ..coli to compensate for genetic perturbations and the utility of combining multiple high throughput data sets to differentiate between causal and noncausal mechanistic changes...
  34. pmc Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis
    Teresa del Castillo
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, E 18008 Granada, Spain
    J Bacteriol 189:5142-52. 2007
    ..It can therefore be concluded that the glucokinase pathway is a sine qua non condition for P. putida to grow with glucose...
  35. pmc Systematic evaluation of objective functions for predicting intracellular fluxes in Escherichia coli
    Robert Schuetz
    Institute of Molecular Systems Biology, ETH Zurich, Switzerland
    Mol Syst Biol 3:119. 2007
    ....
  36. pmc CcpN controls central carbon fluxes in Bacillus subtilis
    Simon Tännler
    Institute of Molecular Systems Biology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 190:6178-87. 2008
    ..In contrast, derepression of sr1, the third known target of CcpN, plays only a marginal role in ccpN mutant phenotypes...
  37. pmc Metabolic flux responses to pyruvate kinase knockout in Escherichia coli
    Marcel Emmerling
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 184:152-64. 2002
    ..Reliability of the flux estimates thus obtained was verified by statistical error analysis and by comparison to intracellular carbon flux ratios that were independently calculated from the same NMR data by metabolic flux ratio analysis...
  38. ncbi request reprint Knockout of the high-coupling cytochrome aa3 oxidase reduces TCA cycle fluxes in Bacillus subtilis
    Nicola Zamboni
    Institute of Biotechnology, ETH Zurich, CH 8093, Zurich, Switzerland
    FEMS Microbiol Lett 226:121-6. 2003
    ..Since the product-corrected biomass yields were identical in mutants and parent, the results show that efficient ATP generation is not overly important for exponential growth of B. subtilis in batch culture...
  39. ncbi request reprint A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli
    Eliane Fischer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Biol Chem 278:46446-51. 2003
    ....
  40. ncbi request reprint Genome engineering reveals large dispensable regions in Bacillus subtilis
    Helga Westers
    Department of Pharmaceutical Biology, University of Groningen, Groningen, The Netherlands
    Mol Biol Evol 20:2076-90. 2003
    ..We show that genome engineering is a feasible strategy for functional analysis of large gene clusters, and that removal of dispensable genomic regions may pave the way toward an optimized Bacillus cell factory...
  41. ncbi request reprint Functional expression of arginine kinase improves recovery from pH stress of Escherichia coli
    Fabrizio Canonaco
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Lett 25:1013-7. 2003
    ..This effect is presumably caused by the physiological function of arginine kinase as a short term energy buffer in the form of phosphoarginine, but a pH-buffering effect cannot be excluded...
  42. ncbi request reprint Reducing maintenance metabolism by metabolic engineering of respiration improves riboflavin production by Bacillus subtilis
    Nicola Zamboni
    Institute of Biotechnology, Swiss Federal Institute of Technology Zurich, ETH Zurich, Honggerberg, CH 8093, Zurich, Switzerland
    Metab Eng 5:49-55. 2003
    ..The reduction of maintenance metabolism, in turn, significantly improved the yield of recombinant riboflavin and B. subtilis biomass in fed-batch cultures...
  43. pmc Evolutionary engineering of Saccharomyces cerevisiae for anaerobic growth on xylose
    Marco Sonderegger
    Institute of Biotechnology, ETH Zurich, Switzerland
    Appl Environ Microbiol 69:1990-8. 2003
    ..However, as a consequence of their improved xylose catabolism, these clones produced up to 19% more ethanol than the parental TMB3001 strain produced under process-like conditions from a mixture of glucose and xylose...
  44. ncbi request reprint Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS
    Eliane Fischer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Eur J Biochem 270:880-91. 2003
    ..Overall, our results provide quantitative insight into flux changes that bring about the resilience of metabolic networks to disruption...
  45. pmc Metabolic-flux profiling of the yeasts Saccharomyces cerevisiae and Pichia stipitis
    Jocelyne Fiaux
    Institute of Molecular Biology and Biophysics, ETH Zurich, CH 8093 Zurich, Switzerland
    Eukaryot Cell 2:170-80. 2003
    ..cerevisiae has a generally high catabolic capacity by overexpressing the Escherichia coli transhydrogenase UdhA in phosphoglucose isomerase-deficient S. cerevisiae...
  46. ncbi request reprint Functional expression of phosphagen kinase systems confers resistance to transient stresses in Saccharomyces cerevisiae by buffering the ATP pool
    Fabrizio Canonaco
    Institute of Biotechnology, Eidgenössiche Technische Hochschule Zurich, CH 8093 Zurich, Switzerland
    J Biol Chem 277:31303-9. 2002
    ..Thus, our results demonstrate that temporal energy buffering is an intrinsic property of phosphagen kinases that can be transferred to phylogenetically very distant organisms...
  47. pmc Intracellular carbon fluxes in riboflavin-producing Bacillus subtilis during growth on two-carbon substrate mixtures
    Michael Dauner
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Appl Environ Microbiol 68:1760-71. 2002
    ..The in vivo activity of the gluconeogenic phosphoenolpyruvate carboxykinase was found to be relatively high in all experiments, with the sole exception of the gluconate-cofed culture...
  48. pmc Molecular basis for anaerobic growth of Saccharomyces cerevisiae on xylose, investigated by global gene expression and metabolic flux analysis
    Marco Sonderegger
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Appl Environ Microbiol 70:2307-17. 2004
    ..We concluded that anaerobic growth of the yeast on xylose is ultimately limited by the rate of ATP production and not by the redox balance per se, although the redox imbalance, in turn, limits ATP production...
  49. ncbi request reprint TCA cycle activity in Saccharomyces cerevisiae is a function of the environmentally determined specific growth and glucose uptake rates
    Lars M Blank
    Institute of Biotechnology, ETH Zurich, Zurich, Switzerland
    Microbiology 150:1085-93. 2004
    ..While sensing of extracellular glucose concentrations has a general influence on the in vivo TCA cycle activity, the growth-rate-dependent increase in respiratory TCA cycle activity was independent of glucose sensing...
  50. pmc Oxygen- and glucose-dependent regulation of central carbon metabolism in Pichia anomala
    Elisabeth Fredlund
    Department of Microbiology, Swedish University of Agricultural Sciences, P O Box 7025, SE 750 07 Uppsala, Sweden
    Appl Environ Microbiol 70:5905-11. 2004
    ..Overall, our results demonstrate that P. anomala exhibits a Pasteur effect and not a Crabtree effect, i.e., oxygen availability, but not glucose concentration, is the main stimulus for the regulation of the central carbon metabolism...
  51. pmc The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway
    Nicola Zamboni
    Institute of Biotechnology, ETH Zurich, Zurich, Switzerland
    J Bacteriol 186:4528-34. 2004
    ....
  52. pmc Efficient classification of complete parameter regions based on semidefinite programming
    Lars Kuepfer
    Institute of Molecular Systems Biology, ETH Zurich, CH 8093 Zurich, Switzerland
    BMC Bioinformatics 8:12. 2007
    ..Consequently, it is impossible to conclude that the "best" solution was identified or that no good solution exists, because parameter spaces typically cannot be explored in a reasonable amount of time...
  53. pmc Metabolic functions of duplicate genes in Saccharomyces cerevisiae
    Lars Kuepfer
    Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland
    Genome Res 15:1421-30. 2005
    ..Thus, at least for metabolism, persistence of the paralog fraction in the genome can be better explained with an array of different, often overlapping functional roles...
  54. pmc Experimental identification and quantification of glucose metabolism in seven bacterial species
    Tobias Fuhrer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Bacteriol 187:1581-90. 2005
    ..Overall, the results suggest that metabolic data from model species with extensive industrial and laboratory history are not representative of microbial metabolism, at least not quantitatively...
  55. ncbi request reprint Ensemble modeling for analysis of cell signaling dynamics
    Lars Kuepfer
    Institute of Molecular Systems Biology, ETH Zurich, 8092 Zurich, Switzerland
    Nat Biotechnol 25:1001-6. 2007
    ..Beyond revising our picture of TOR signaling, we expect ensemble modeling to help elucidate other insufficiently characterized cellular circuits...
  56. ncbi request reprint Robustness of cellular functions
    Jörg Stelling
    Max Planck Institute for Dynamics of Complex Technical Systems, D 39106 Magdeburg, Germany
    Cell 118:675-85. 2004
    ..Robustness may be a key to understanding cellular complexity, elucidating design principles, and fostering closer interactions between experimentation and theory...
  57. ncbi request reprint Evolutionary engineering of multiple-stress resistant Saccharomyces cerevisiae
    Z Petek Cakar
    Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Technical University, Maslak, TR 34469 Istanbul, Turkey
    FEMS Yeast Res 5:569-78. 2005
    ..General selection guidelines for improving multiple-stress resistance in S. cerevisiae are presented and discussed...
  58. ncbi request reprint High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints
    Eliane Fischer
    Institute of Biotechnology, ETH Zürich CH 8093, Zurich, Switzerland
    Anal Biochem 325:308-16. 2004
    ..coli shake flasks and 1-ml deep-well microtiter plates. Due to its simplicity, speed, and robustness, 13C-constrained metabolic flux balancing is promising for routine and high-throughput analysis on a miniaturized scale...
  59. ncbi request reprint Large-scale in vivo flux analysis shows rigidity and suboptimal performance of Bacillus subtilis metabolism
    Eliane Fischer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Nat Genet 37:636-40. 2005
    ..Network rigidity and robustness are probably universal functional design principles, whereas the standby mode may be more specific...
  60. ncbi request reprint Long-term continuous evolution of acetate resistant Acetobacter aceti
    Peter Steiner
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Bioeng 84:40-4. 2003
    ..Intracellular acetate concentrations were significantly lower in evolved A. aceti, when compared to wild-type A. aceti and E. coli, indicating that cytoplasmatic anion accumulation is an important component of acetate toxicity...
  61. ncbi request reprint Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae strains
    Marco Sonderegger
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Bioeng 87:90-8. 2004
    ....
  62. pmc Metabolic engineering of a phosphoketolase pathway for pentose catabolism in Saccharomyces cerevisiae
    Marco Sonderegger
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Appl Environ Microbiol 70:2892-7. 2004
    ..By combining the phosphoketolase pathway with the ald6 mutation, which reduced acetate formation, a strain with an ethanol yield 20% higher and a xylose fermentation rate 40% higher than those of its parent was engineered...
  63. ncbi request reprint The phosphoenolpyruvate carboxykinase also catalyzes C3 carboxylation at the interface of glycolysis and the TCA cycle of Bacillus subtilis
    Nicola Zamboni
    Institute of Biotechnology, Swiss Federal Institute of Technology Zurich ETH ETH Zürich, Zurich 8093, Switzerland
    Metab Eng 6:277-84. 2004
    ..Presumably as a consequence of the unfavorable kinetics of an ATP-synthesizing anaplerotic PEP carboxykinase reaction, such pyruvate carboxylase mutants grow slowly or, as in the case of wild-type 168, not at all...