Nicolas Ruggli

Summary

Affiliation: Swiss Federal Research Station
Country: Switzerland

Publications

  1. pmc Classical swine fever virus interferes with cellular antiviral defense: evidence for a novel function of N(pro)
    Nicolas Ruggli
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Virol 77:7645-54. 2003
  2. ncbi request reprint N(pro) of classical swine fever virus is an antagonist of double-stranded RNA-mediated apoptosis and IFN-alpha/beta induction
    Nicolas Ruggli
    Institute of Virology and Immunoprophylaxis IVI, Mittelhausern, Switzerland
    Virology 340:265-76. 2005
  3. pmc Efficient sensing of infected cells in absence of virus particles by plasmacytoid dendritic cells is blocked by the viral ribonuclease E(rns.)
    Sylvie Python
    Institute of Virology and Immunology, Mittelhausern, Switzerland
    PLoS Pathog 9:e1003412. 2013
  4. pmc MDA5 can be exploited as efficacious genetic adjuvant for DNA vaccination against lethal H5N1 influenza virus infection in chickens
    Matthias Liniger
    Research Department, Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    PLoS ONE 7:e49952. 2012
  5. pmc Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3-degrading function of Npro
    Nicolas Ruggli
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Virol 83:817-29. 2009
  6. ncbi request reprint Role of double-stranded RNA and Npro of classical swine fever virus in the activation of monocyte-derived dendritic cells
    Oliver Bauhofer
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Virology 343:93-105. 2005
  7. pmc Classical swine fever virus N(pro) limits type I interferon induction in plasmacytoid dendritic cells by interacting with interferon regulatory factor 7
    Ana R Fiebach
    Institute of Virology and Immunoprophylaxis IVI, Mittelhausern, Switzerland
    J Virol 85:8002-11. 2011
  8. ncbi request reprint Classical swine fever virus replicon particles lacking the Erns gene: a potential marker vaccine for intradermal application
    Caroline F Frey
    Institute of Virology and Immunoprophylaxis, 3147 Mittelhäusern, Switzerland
    Vet Res 37:655-70. 2006
  9. pmc High interferon type I responses in the lung, plasma and spleen during highly pathogenic H5N1 infection of chicken
    Hervé R Moulin
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland
    Vet Res 42:6. 2011
  10. doi request reprint N(pro) of classical swine fever virus prevents type I interferon-mediated priming of conventional dendritic cells for enhanced interferon-α response
    Linda Hüsser
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Interferon Cytokine Res 32:221-9. 2012

Collaborators

Detail Information

Publications29

  1. pmc Classical swine fever virus interferes with cellular antiviral defense: evidence for a novel function of N(pro)
    Nicolas Ruggli
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Virol 77:7645-54. 2003
    ..Taken together, these results provide evidence for a novel function associated with CSFV N(pro) with respect to the inhibition of the cellular innate immune system...
  2. ncbi request reprint N(pro) of classical swine fever virus is an antagonist of double-stranded RNA-mediated apoptosis and IFN-alpha/beta induction
    Nicolas Ruggli
    Institute of Virology and Immunoprophylaxis IVI, Mittelhausern, Switzerland
    Virology 340:265-76. 2005
    ..This establishes a novel function for N(pro) in counteraction of the IFN-alpha/beta induction pathway...
  3. pmc Efficient sensing of infected cells in absence of virus particles by plasmacytoid dendritic cells is blocked by the viral ribonuclease E(rns.)
    Sylvie Python
    Institute of Virology and Immunology, Mittelhausern, Switzerland
    PLoS Pathog 9:e1003412. 2013
    ..We propose that this pathway of activation could be of particular importance for viruses which tend to be mostly cell-associated, cause persistent infection, and are non-cytopathogenic...
  4. pmc MDA5 can be exploited as efficacious genetic adjuvant for DNA vaccination against lethal H5N1 influenza virus infection in chickens
    Matthias Liniger
    Research Department, Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    PLoS ONE 7:e49952. 2012
    ..These data demonstrate that innate immune activation by expression of signaling domains of RIG-I-like receptors can be exploited to enhance vaccine efficacy...
  5. pmc Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3-degrading function of Npro
    Nicolas Ruggli
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Virol 83:817-29. 2009
    ..Taken together, these results demonstrate that contrary to previous assumptions and to the case for other viral systems, impairment of IRF3-dependent IFN-alpha/beta induction is not a prerequisite for CSFV virulence...
  6. ncbi request reprint Role of double-stranded RNA and Npro of classical swine fever virus in the activation of monocyte-derived dendritic cells
    Oliver Bauhofer
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Virology 343:93-105. 2005
    ..In conclusion, the capacity of CSFV to replicate in myeloid DC, and prevent IFN-alpha/beta induction and DC maturation, requires both regulated dsRNA levels and the presence of viral Npro...
  7. pmc Classical swine fever virus N(pro) limits type I interferon induction in plasmacytoid dendritic cells by interacting with interferon regulatory factor 7
    Ana R Fiebach
    Institute of Virology and Immunoprophylaxis IVI, Mittelhausern, Switzerland
    J Virol 85:8002-11. 2011
    ..Taken together, the results of this study show that CSFV N(pro) is capable of manipulating the function of IRF7 in pDC and provides the virus with an additional strategy to circumvent the innate defense...
  8. ncbi request reprint Classical swine fever virus replicon particles lacking the Erns gene: a potential marker vaccine for intradermal application
    Caroline F Frey
    Institute of Virology and Immunoprophylaxis, 3147 Mittelhäusern, Switzerland
    Vet Res 37:655-70. 2006
    ....
  9. pmc High interferon type I responses in the lung, plasma and spleen during highly pathogenic H5N1 infection of chicken
    Hervé R Moulin
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland
    Vet Res 42:6. 2011
    ..Our data also indicate that the viral load as well as the cleavability of the HA enabling systemic spread of the virus are two major factors controlling systemic IFN responses in chicken...
  10. doi request reprint N(pro) of classical swine fever virus prevents type I interferon-mediated priming of conventional dendritic cells for enhanced interferon-α response
    Linda Hüsser
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Interferon Cytokine Res 32:221-9. 2012
    ..These results indicate that CSFV has evolved to prevent type I IFN sensitization in infected cells through the action of the N(pro)...
  11. doi request reprint Immunogenic and replicative properties of classical swine fever virus replicon particles modified to induce IFN-α/β and carry foreign genes
    Rolf Suter
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Vaccine 29:1491-503. 2011
    ..Importantly, the IFN-α/β-inducing VRP did show increased efficacy over the unmodified VRP in terms of B-cell and T-cell responses, when tested with secondary immune responses by in vitro restimulation assay...
  12. ncbi request reprint Responsiveness of fibrocytes to toll-like receptor danger signals
    Carole Balmelli
    Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH 3147, Mittelhausern, Switzerland
    Immunobiology 212:693-9. 2007
    ..Considering the rapid entry of fibrocytes into wounds, this efficient responsiveness to TLR danger signals, reflects a potentially important role of these cells in the first line of defence against pathogen invasion following traumata...
  13. ncbi request reprint Efficacy and functionality of lipoprotein OprI from Pseudomonas aeruginosa as adjuvant for a subunit vaccine against classical swine fever
    Henriette Rau
    Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Vaccine 24:4757-68. 2006
    ..Further studies are necessary to optimize antigen formulation enabling to translate the in vitro efficacy into a potent vaccine in vivo...
  14. pmc Classical swine fever virus Npro interacts with interferon regulatory factor 3 and induces its proteasomal degradation
    Oliver Bauhofer
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    J Virol 81:3087-96. 2007
    ..Furthermore, N(pro) coprecipitates with IRF3, suggesting that the proteasomal degradation of IRF3 is induced by a direct or indirect interaction with N(pro). Finally, we show that N(pro) does not downregulate IRF7 expression...
  15. pmc Highly pathogenic avian influenza virus H5N1 controls type I IFN induction in chicken macrophage HD-11 cells: a polygenic trait that involves NS1 and the polymerase complex
    Matthias Liniger
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    Virol J 9:7. 2012
    ..Therefore, the type I IFN responses induced by highly virulent and low virulent H5N1 AIV and reassortants thereof were analysed in chicken cells...
  16. doi request reprint Propagation of classical swine fever virus in vitro circumventing heparan sulfate-adaptation
    Rita Eymann-Häni
    Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    J Virol Methods 176:85-95. 2011
    ..The importance of these findings can be seen from the altered ratio of cell-associated to secreted virus upon acquisition of enhanced HS-binding affinity, a phenotype proposed previously to be related to virulence in the natural host...
  17. pmc Hemagglutinin-dependent tropism of H5N1 avian influenza virus for human endothelial cells
    Manuela Ocaña-Macchi
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    J Virol 83:12947-55. 2009
    ..Overall, avian H5N1 viruses have a particular receptor specificity targeting endothelial cells that is different from human influenza viruses, and this H5N1 receptor specificity could contribute to disease pathogenesis...
  18. doi request reprint Approaches to define the viral genetic basis of classical swine fever virus virulence
    Immanuel Leifer
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Virology 438:51-5. 2013
    ..The topic of virulence is addressed from different angles; nonconventional approaches like codon pair usage and quasispecies are considered. Future research approaches in the field of CSFV virulence are proposed...
  19. ncbi request reprint Oronasal vaccination with classical swine fever virus (CSFV) replicon particles with either partial or complete deletion of the E2 gene induces partial protection against lethal challenge with highly virulent CSFV
    Roland Maurer
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    Vaccine 23:3318-28. 2005
    ..In addition, our data suggest that E2 of CSFV is dispensable for the induction of mucosal but not of parenteral immunity...
  20. ncbi request reprint Nonstructural proteins NS2-3 and NS4A of classical swine fever virus: essential features for infectious particle formation
    Hervé R Moulin
    Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Virology 365:376-89. 2007
    ..These data indicate that NS2-3 and NS3, each in association with NS4A, have independent functions in the CSFV life cycle...
  21. ncbi request reprint Mycoplasma contamination and viral immunomodulatory activity: dendritic cells open Pandora's box
    Marco P Alves
    Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Immunol Lett 110:101-9. 2007
    ....
  22. pmc Chicken cells sense influenza A virus infection through MDA5 and CARDIF signaling involving LGP2
    Matthias Liniger
    Institute of Virology and Immunoprophylaxis IVI, Mittelhausern, Switzerland
    J Virol 86:705-17. 2012
    ..We finally demonstrate that the chMDA5 signaling pathway is inhibited by the viral nonstructural protein 1. In conclusion, chicken cells, including DF-1 fibroblasts and HD-11 macrophage-like cells, employ chMDA5 for sensing AIV...
  23. ncbi request reprint Dendritic cells--at the front-line of pathogen attack
    Kenneth C McCullough
    Institute of Virology and Immunoprophylaxis, Research Department, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Vet Immunol Immunopathol 128:7-15. 2009
    ..Overall, the roles played by the pDC can be decisive in shaping the outcome of the infection and the characteristics of the virus-induced immunocompromisation...
  24. ncbi request reprint Functional RNA delivery targeted to dendritic cells by synthetic nanoparticles
    Kenneth C McCullough
    Institute of Virology and Immunoprophylaxis, CH 3147 Mittelhäusern, Switzerland
    Ther Deliv 3:1077-99. 2012
    ..The potential advantages for application of replicating RNA are presented and discussed, in consideration of their value and development in the near future...
  25. pmc Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes
    Matthias Mueller
    Institute of Virology and Immunoprophylaxis IVI, Mittelhaeusern, Switzerland
    PLoS ONE 5:e9097. 2010
    ..Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA...
  26. doi request reprint Identification of the role of RIG-I, MDA-5 and TLR3 in sensing RNA viruses in porcine epithelial cells using lentivirus-driven RNA interference
    Linda Hüsser
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    Virus Res 159:9-16. 2011
    ....
  27. ncbi request reprint Fibrocytes are potent stimulators of anti-virus cytotoxic T cells
    Carole Balmelli
    Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland
    J Leukoc Biol 77:923-33. 2005
    ..Although IFN-gamma pretreatment of Fb was not necessary for this function, it could enhance the Fb activity. These results demonstrate that Fb are efficient, accessory cells for the presentation of viral antigen to specific CD8(+) CTL...
  28. pmc Clustering of classical swine fever virus isolates by codon pair bias
    Immanuel Leifer
    Institute of Virology and Immunoprophylaxis IVI, Sensemattstrasse 293, CH 3147 Mittelhäusern, Switzerland
    BMC Res Notes 4:521. 2011
    ..abstract:..
  29. ncbi request reprint Properties of H7N7 influenza A virus strain SC35M lacking interferon antagonist NS1 in mice and chickens
    Georg Kochs
    Department of Virology, University of Freiburg, 79104 Freiburg, Germany
    J Gen Virol 88:1403-9. 2007
    ..Altogether, this study supports the view that NS1 represents a non-essential virulence factor of different influenza A viruses...