Serge Pelet

Summary

Affiliation: Swiss Federal Institute of Technology
Country: Switzerland

Publications

  1. ncbi An integrated image analysis platform to quantify signal transduction in single cells
    Serge Pelet
    ETH Zurich, Institute of Biochemistry, Schafmattstr 18, CH 8093 Zurich, Switzerland
    Integr Biol (Camb) 4:1274-82. 2012
  2. ncbi Comparing the quantification of Forster resonance energy transfer measurement accuracies based on intensity, spectral, and lifetime imaging
    Serge Pelet
    Massachusetts Institute of Technology, Department of Mechanical Engineering, Cambridge, Massachusetts 02139, USA
    J Biomed Opt 11:34017. 2006
  3. doi Transient activation of the HOG MAPK pathway regulates bimodal gene expression
    Serge Pelet
    ETH Zurich, Department of Biology, Institute of Biochemistry, Schafmattstrasse 18, CH 8093 Zurich, Switzerland
    Science 332:732-5. 2011
  4. doi Scalable inference of heterogeneous reaction kinetics from pooled single-cell recordings
    Christoph Zechner
    Automatic Control Lab, ETH Zurich, Zurich, Switzerland
    Nat Methods 11:197-202. 2014
  5. pmc Moment-based inference predicts bimodality in transient gene expression
    Christoph Zechner
    Automatic Control Laboratory, ETH Zurich, CH 8092 Zurich, Switzerland
    Proc Natl Acad Sci U S A 109:8340-5. 2012
  6. doi Quantitative and dynamic assay of single cell chemotaxis
    Sung Sik Lee
    Institute of Biochemistry, ETH Zurich, Zurich, CH 8093, Switzerland
    Integr Biol (Camb) 4:381-90. 2012
  7. pmc Cytosolic pH is a second messenger for glucose and regulates the PKA pathway through V-ATPase
    Reinhard Dechant
    Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
    EMBO J 29:2515-26. 2010
  8. pmc Quantifying intracellular protein binding thermodynamics during mechanotransduction based on FRET spectroscopy
    Nur Aida Abdul Rahim
    Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Mass Ave, Cambridge, MA 02139, United States
    Methods 66:208-21. 2014
  9. pmc Methodological considerations for global analysis of cellular FLIM/FRET measurements
    Nur Aida Adbul Rahim
    Massachusetts Institute of Technology, Department of Mechanical Engineering, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    J Biomed Opt 17:026013. 2012
  10. doi Spectrally resolved fluorescence correlation spectroscopy based on global analysis
    Michael J R Previte
    Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Anal Chem 80:3277-84. 2008

Collaborators

Detail Information

Publications11

  1. ncbi An integrated image analysis platform to quantify signal transduction in single cells
    Serge Pelet
    ETH Zurich, Institute of Biochemistry, Schafmattstr 18, CH 8093 Zurich, Switzerland
    Integr Biol (Camb) 4:1274-82. 2012
    ..Such dynamic single cell measurements are highly needed to establish mathematical models of signal transduction pathways...
  2. ncbi Comparing the quantification of Forster resonance energy transfer measurement accuracies based on intensity, spectral, and lifetime imaging
    Serge Pelet
    Massachusetts Institute of Technology, Department of Mechanical Engineering, Cambridge, Massachusetts 02139, USA
    J Biomed Opt 11:34017. 2006
    ..The latter proves to be the best method to quantify FRET efficiency in the image. More importantly, the intensity fraction of molecules undergoing FRET can be quantitatively measured in each pixel of the image...
  3. doi Transient activation of the HOG MAPK pathway regulates bimodal gene expression
    Serge Pelet
    ETH Zurich, Department of Biology, Institute of Biochemistry, Schafmattstrasse 18, CH 8093 Zurich, Switzerland
    Science 332:732-5. 2011
    ..Together, these findings provide a molecular mechanism by which a cell can impose a transcriptional threshold in response to a linear signaling behavior...
  4. doi Scalable inference of heterogeneous reaction kinetics from pooled single-cell recordings
    Christoph Zechner
    Automatic Control Lab, ETH Zurich, Zurich, Switzerland
    Nat Methods 11:197-202. 2014
    ..We use the method to reconstruct the expression dynamics of a gene under an inducible promoter in yeast from time-lapse microscopy data. ..
  5. pmc Moment-based inference predicts bimodality in transient gene expression
    Christoph Zechner
    Automatic Control Laboratory, ETH Zurich, CH 8092 Zurich, Switzerland
    Proc Natl Acad Sci U S A 109:8340-5. 2012
    ..Our results show that measurements of the mean and the variance can be enough to determine the model parameters, even if the measured distributions are not well-characterized by low-order moments only--e.g., if they are bimodal...
  6. doi Quantitative and dynamic assay of single cell chemotaxis
    Sung Sik Lee
    Institute of Biochemistry, ETH Zurich, Zurich, CH 8093, Switzerland
    Integr Biol (Camb) 4:381-90. 2012
    ....
  7. pmc Cytosolic pH is a second messenger for glucose and regulates the PKA pathway through V-ATPase
    Reinhard Dechant
    Institute of Biochemistry, ETH Zurich, Zurich, Switzerland
    EMBO J 29:2515-26. 2010
    ..Thus, these data suggest a novel and potentially conserved glucose-sensing pathway and identify a mechanism how cytosolic pH can act as a signal to promote cell growth...
  8. pmc Quantifying intracellular protein binding thermodynamics during mechanotransduction based on FRET spectroscopy
    Nur Aida Abdul Rahim
    Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Mass Ave, Cambridge, MA 02139, United States
    Methods 66:208-21. 2014
    ....
  9. pmc Methodological considerations for global analysis of cellular FLIM/FRET measurements
    Nur Aida Adbul Rahim
    Massachusetts Institute of Technology, Department of Mechanical Engineering, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    J Biomed Opt 17:026013. 2012
    ....
  10. doi Spectrally resolved fluorescence correlation spectroscopy based on global analysis
    Michael J R Previte
    Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Anal Chem 80:3277-84. 2008
    ..We have demonstrated that the global analysis approach allows the concentration and diffusion coefficients of fluorescent particles to be resolved despite the presence of overlapping emission spectra...
  11. pmc Dynamic processes at stress promoters regulate the bimodal expression of HOG response genes
    Serge Pelet
    Institute of Biochemistry, Department of Biology, ETHZ Competence Center for Systems Physiology and Metabolic Diseases CC SPMD Zürich, Switzerland
    Commun Integr Biol 4:699-702. 2011
    ..Interestingly, a slow stochastic gene activation process is responsible for setting a tunable threshold for gene expression under basal or low stress conditions, which generates a bimodal expression pattern at intermediate stress levels...