Pier Luigi Luisi

Summary

Affiliation: Swiss Federal Institute of Technology
Country: Switzerland

Publications

  1. ncbi request reprint Basic questions about the origins of life: proceedings of the Erice international school of complexity (fourth course)
    Pasquale Stano
    Research Centre Enrico Fermi, Compendio del Viminale, 00184, Rome, Italy
    Orig Life Evol Biosph 37:303-7. 2007
  2. pmc Quasi-cellular systems: stochastic simulation analysis at nanoscale range
    Lorenzo Calviello
    Dipartimento di Informatica, Universita di Pisa, L go B Pontecorvo 3, 56127 Pisa, Italy
    BMC Bioinformatics 14:S7. 2013
  3. doi request reprint An open question on the origin of life: the first forms of metabolism
    Pier Luigi Luisi
    Biology Department, University of Roma Tre, Viale Guglielmo Marconi 446, IT 00146 Rome, Italy
    Chem Biodivers 9:2635-47. 2012
  4. doi request reprint Vesicle behavior: in search of explanations
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Rome, Italy
    J Phys Chem B 112:14655-64. 2008
  5. ncbi request reprint Question 3: the problem of macromolecular sequences: the forgotten stumbling block
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Viale G Marconi 446, 00146, Rome Italy
    Orig Life Evol Biosph 37:363-5. 2007
  6. ncbi request reprint Chemical aspects of synthetic biology
    Pier Luigi Luisi
    Department Biology, University of Roma Tre, Viale G Marconi 446, I 00146 Roma
    Chem Biodivers 4:603-21. 2007
  7. ncbi request reprint From Never Born Proteins to Minimal Living Cells: two projects in synthetic biology
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Viale G Marconi 446, 00146 Rome, Italy
    Orig Life Evol Biosph 36:605-16. 2006
  8. ncbi request reprint Approaches to semi-synthetic minimal cells: a review
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Viale G Marconi 446, 00146 Rome, Italy
    Naturwissenschaften 93:1-13. 2006
  9. ncbi request reprint A possible route to prebiotic vesicle reproduction
    Pier Luigi Luisi
    Dipartimento di Biologia, Universita degli Studi Roma Tre, Italy
    Artif Life 10:297-308. 2004
  10. doi request reprint Approaches to chemical synthetic biology
    Cristiano Chiarabelli
    Biology Department, University of Roma Tre, Viale Guglielmo Marconi 446, 00146 Rome, Italy
    FEBS Lett 586:2138-45. 2012

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Basic questions about the origins of life: proceedings of the Erice international school of complexity (fourth course)
    Pasquale Stano
    Research Centre Enrico Fermi, Compendio del Viminale, 00184, Rome, Italy
    Orig Life Evol Biosph 37:303-7. 2007
  2. pmc Quasi-cellular systems: stochastic simulation analysis at nanoscale range
    Lorenzo Calviello
    Dipartimento di Informatica, Universita di Pisa, L go B Pontecorvo 3, 56127 Pisa, Italy
    BMC Bioinformatics 14:S7. 2013
    ..A suitable model formally describing the PS reactions network was developed, to predict, from inner species concentrations (very difficult to measure in small-volumes), the resulting fluorescence signal (experimentally observable)...
  3. doi request reprint An open question on the origin of life: the first forms of metabolism
    Pier Luigi Luisi
    Biology Department, University of Roma Tre, Viale Guglielmo Marconi 446, IT 00146 Rome, Italy
    Chem Biodivers 9:2635-47. 2012
    ..It is argued that this spontaneous overcrowding, which permits reactions which are not possible in the original dilute solution, might be the origin of cellular metabolism for the origin of life on Earth...
  4. doi request reprint Vesicle behavior: in search of explanations
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Rome, Italy
    J Phys Chem B 112:14655-64. 2008
    ....
  5. ncbi request reprint Question 3: the problem of macromolecular sequences: the forgotten stumbling block
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Viale G Marconi 446, 00146, Rome Italy
    Orig Life Evol Biosph 37:363-5. 2007
    ..Such constraints are examined and discussed...
  6. ncbi request reprint Chemical aspects of synthetic biology
    Pier Luigi Luisi
    Department Biology, University of Roma Tre, Viale G Marconi 446, I 00146 Roma
    Chem Biodivers 4:603-21. 2007
    ..These minimal cells are not yet capable of self-reproduction, and this and other shortcomings within the project are critically reviewed...
  7. ncbi request reprint From Never Born Proteins to Minimal Living Cells: two projects in synthetic biology
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Viale G Marconi 446, 00146 Rome, Italy
    Orig Life Evol Biosph 36:605-16. 2006
    ..Results and outlooks on these two research lines are shortly discussed, mainly focusing on their relevance to the origin of life studies...
  8. ncbi request reprint Approaches to semi-synthetic minimal cells: a review
    Pier Luigi Luisi
    Biology Department, University of RomaTre, Viale G Marconi 446, 00146 Rome, Italy
    Naturwissenschaften 93:1-13. 2006
    ..In conclusion, we give a brief outline of the next possible steps on the road map to the minimal cell...
  9. ncbi request reprint A possible route to prebiotic vesicle reproduction
    Pier Luigi Luisi
    Dipartimento di Biologia, Universita degli Studi Roma Tre, Italy
    Artif Life 10:297-308. 2004
    ..Preliminary data also show that repeated addition of oleate maintains the same basic initial features, and that surfactants other than oleate also respect the reproductive mode of the matrix effect...
  10. doi request reprint Approaches to chemical synthetic biology
    Cristiano Chiarabelli
    Biology Department, University of Roma Tre, Viale Guglielmo Marconi 446, 00146 Rome, Italy
    FEBS Lett 586:2138-45. 2012
    ..We describe and critically analyze the main results, emphasizing the possible relevance of chemical synthetic biology for the progress in basic science and biotechnology...
  11. doi request reprint Biosynthesis of proteins inside liposomes
    Pasquale Stano
    Biology Department, University of RomaTre, Rome, Italy
    Methods Mol Biol 606:127-45. 2010
    ..On this basis, we provide general indications for designing and realizing protein-expressing liposomes. Our approach for the green fluorescent protein expression inside 200-nm extruded vesicles is described in detail...
  12. doi request reprint Chemical approaches to synthetic biology
    Cristiano Chiarabelli
    Biology Department, University of Roma Tre, Viale G Marconi 446, Rome I 00146, Italy
    Curr Opin Biotechnol 20:492-7. 2009
    ....
  13. doi request reprint Achievements and open questions in the self-reproduction of vesicles and synthetic minimal cells
    Pasquale Stano
    Biology Department, University of RomaTre, Italy
    Chem Commun (Camb) 46:3639-53. 2010
    ..This approach aims to investigate the minimal requirements for molecular systems in order to display some living properties, while it finds relevance in origins of life studies and in synthetic (constructive) biology...
  14. ncbi request reprint Toward the engineering of minimal living cells
    Pier Luigi Luisi
    Institute of Polymers, Swiss Federal Institute of Technology, ETH Zurich, Switzerland
    Anat Rec 268:208-14. 2002
    ..In the final part of the article, the outlook of increasing the complexity of these liposome systems so as to arrive at first semi-synthetic cells is discussed...
  15. ncbi request reprint Autopoiesis: a review and a reappraisal
    Pier Luigi Luisi
    Institut fur Polymere, Swiss Federal Institute of Technology, 8092, Zurich, Switzerland
    Naturwissenschaften 90:49-59. 2003
    ..Finally, it is pointed out that the new interest in system biology and complexity theories may lead to a reappraisal of autopoiesis and related notions, as outlined also by other authors, such as Tibor Ganti and Stuart Kauffmann...
  16. ncbi request reprint Size distribution of spontaneously formed liposomes by the alcohol injection method
    Anastasia S Domazou
    Institute of Polymers, ETH Zentrum, Zurich, CH 8092, Switzerland
    J Liposome Res 12:205-20. 2002
    ..It appears that the larger components in the light scattering results are not time-stable, whereas the smaller liposome species are. It is shown that these features are also present when alcohols different from methanol are used...
  17. doi request reprint Giant vesicles "colonies": a model for primitive cell communities
    Paolo Carrara
    Biology Department, Universita degli Studi Roma Tre, Viale Guglielmo Marconi 446, 00146 Rome, Italy
    Chembiochem 13:1497-502. 2012
    ..In fact there are two novel unexpected features typical of vesicle colonies, namely solute capture and vesicle fusion, which can be seen as the basic physicochemical mechanisms at the origin of life...
  18. doi request reprint Semi-synthetic minimal cells as a tool for biochemical ICT
    Pasquale Stano
    Biology Department, University of Roma Tre, Rome, Italy
    Biosystems 109:24-34. 2012
    ....
  19. doi request reprint The minimal size of liposome-based model cells brings about a remarkably enhanced entrapment and protein synthesis
    Tereza Pereira de Souza
    Department of Biology, University of RomaTre, Rome, Italy
    Chembiochem 10:1056-63. 2009
    ..We conclude by suggesting that these entrapment effects in small-sized compartments, once validated, might be very relevant in the origin-of-life scenario...
  20. doi request reprint Semi-synthetic minimal cells: origin and recent developments
    Pasquale Stano
    Biology Department, University of Roma Tre, Viale G Marconi 446, I 00146 Rome, Italy
    Curr Opin Biotechnol 24:633-8. 2013
    ..The role of physicochemical understandings is greatly emphasized. ..
  21. doi request reprint Reactivity and fusion between cationic vesicles and fatty acid anionic vesicles
    Filippo Caschera
    Biology Department, University of RomaTre, Viale G Marconi 446, I 00146 Rome, Italy
    J Colloid Interface Sci 345:561-5. 2010
    ..This study also contributes to clarify the term "vesicle fusion", which is not always properly used in describing reactivity among vesicles...
  22. pmc Massive non-natural proteins structure prediction using grid technologies
    Giovanni Minervini
    Department of Biology, University Roma Tre, Viale G, Marconi 446, Rome, I 00146, Italy
    BMC Bioinformatics 10:S22. 2009
    ..Thus, the structural study of NBPs can help to understand if natural protein sequences were selected for their peculiar properties or if they are just one of the possible stable and functional ensembles...
  23. doi request reprint Ser-His catalyses the formation of peptides and PNAs
    Maçha Gorlero
    Biology Department, University of RomaTre, Rome, Italy
    FEBS Lett 583:153-6. 2009
    ..Other simpler peptides as Gly-Gly, or Gly-Gly-Gly are also effective, although less efficiently. We discuss the results from the viewpoint of primitive chemistry and the origin of long macromolecules by stepwise fragment condensations...
  24. ncbi request reprint RNA selectively interacts with vesicles depending on their size
    Chris F Thomas
    Dipartimento di Biologia, Universita degli Studi Roma Tre, Viale Guglielmo Marconi 446, 00146 Rome, Italy
    J Phys Chem B 109:14544-50. 2005
    ..This kind of discrimination capability of RNA might have been of importance in the early phases of the formation of the protocells...
  25. ncbi request reprint Contingency and determinism
    Pier Luigi Luisi
    Department of Materials, ETH Zentrum, 8092 Zurich, Switzerland
    Philos Transact A Math Phys Eng Sci 361:1141-7. 2003
    ..Contingency versus determinism is an old, classic issue in the history of science, and certain trends of contemporary science literature appear to be bringing it to the foreground again...
  26. ncbi request reprint The production of de novo folded proteins by a stepwise chain elongation: a model for prebiotic chemical evolution of macromolecular sequences
    Salvatore Chessari
    Department of Materials, Swiss Federal Institute of Technology, ETHZ, CH 8093 Zurich
    Chem Biodivers 3:1202-10. 2006
    ..The implication of this procedure as a general mechanism for the etiology of de novo macromolecular sequences and globular proteins in the origin of life is briefly discussed...
  27. ncbi request reprint Condensed DNA in lipid microcompartments
    Shahriar Osfouri
    Institut fur Polymere, ETH Zentrum, Universitatstrasse 6, CH 8092 Zurich, Switzerland
    J Phys Chem B 109:19929-35. 2005
    ..600 mg/mL, comparable to that found in restricted biological compartments...
  28. ncbi request reprint Effect of tryptophan oligopeptides on the size distribution of POPC liposomes: a dynamic light scattering and turbidimetric study
    Pasquale Stano
    Institut fur Polymere, ETH Zentrum, Zurich, Switzerland
    J Liposome Res 15:29-47. 2005
    ....
  29. ncbi request reprint Novel camptothecin analogue (gimatecan)-containing liposomes prepared by the ethanol injection method
    Pasquale Stano
    Institute of Polymers, ETH Zentrum, Universitatstrasse 6, Zurich, Switzerland
    J Liposome Res 14:87-109. 2004
    ..05). Finally, our study showed that the liposomal formulation was able to realize a delivery system of a water-insoluble drug, providing a Gimatecan formulation for intravenous administration with a preserved antitumoral activity...
  30. ncbi request reprint Protein synthesis in liposomes with a minimal set of enzymes
    Giovanni Murtas
    Centro Studi e Ricerche E Fermi, Compendio Viminale, 00184 Rome, Italy
    Biochem Biophys Res Commun 363:12-7. 2007
    ..The expression of GPAT and LPAAT in liposomes would in principle allow the production of the cell boundary from within...
  31. ncbi request reprint Investigation of de novo totally random biosequences, Part IV: Folding Properties of de novo, totally random RNAs
    Davide De Lucrezia
    Department of Biology, University of Roma Tre, Viale G Marconi 446, IT 00146 Rome
    Chem Biodivers 3:869-77. 2006
    ..The results are discussed within the general framework of random RNA properties such as those that might have been produced in a prebiotic scenario...
  32. ncbi request reprint Investigation of de novo totally random biosequences, Part III: RNA Foster: A novel assay to investigate RNA folding structural properties
    Davide De Lucrezia
    Department of Biology, University of Roma Tre, Viale G Marconi 446, IT 00146 Rome
    Chem Biodivers 3:860-8. 2006
    ..The simplicity of RNA Foster suggests that it can easily be scaled up for high-throughput studies of RNA folding stability both in basic and applied research...
  33. ncbi request reprint Investigation of de novo totally random biosequences, Part II: On the folding frequency in a totally random library of de novo proteins obtained by phage display
    Cristiano Chiarabelli
    Centro Studi e Ricerche E Fermi, Via Panisperna 89A, IT 00184 Rome
    Chem Biodivers 3:840-59. 2006
    ..The consequences of this finding within a library of 'Never Born Proteins' are discussed in terms of molecular evolution...
  34. ncbi request reprint Cell-free protein synthesis through solubilisate exchange in water/oil emulsion compartments
    Adriana V Pietrini
    Institute of Polymers, ETH Zentrum, Universitatsstrasse 6, 8092 Zurich, Switzerland
    Chembiochem 5:1055-62. 2004
    ..8 ng microL(-1) protein after 5 h. The results with the w/o emulsions show that solubilisate exchange takes place among the different water droplets, but it is not possible to demonstrate clearly that a true fusion takes place...
  35. ncbi request reprint Comparison of phosphatidylcholine vesicle properties related to geometrical isomerism
    Carla Ferreri
    ISOF, Consiglio Nazionale delle Ricerche, Via P Gobetti 101, 40129 Bologna, Italy
    Photochem Photobiol 82:274-80. 2006
    ..A picture emerged of the geometrical isomer preference in cells as a process driven by natural selection during the life evolution of different organisms, both in terms of compartment dimensions and membrane functionality...
  36. ncbi request reprint Circular dichroic properties and average dimensions of DNA-containing reverse micellar aggregates
    Adriana V Pietrini
    Institute for Polymers, ETH Zentrum, Zurich, Switzerland
    Biochim Biophys Acta 1562:57-62. 2002
    ....