Affiliation: Swiss Federal Institute of Technology
Ungricht R, Klann M, Horvath P, Kutay U. Diffusion and retention are major determinants of protein targeting to the inner nuclear membrane. J Cell Biol. 2015;209:687-703 pubmed publisher
..We experimentally confirmed the sufficiency of diffusion and retention using an artificial reporter lacking natural sorting signals that recapitulates the energy dependence of the process in vivo. ..
Ungricht R, Kutay U. Establishment of NE asymmetryâ€”targeting of membrane proteins to the inner nuclear membrane. Curr Opin Cell Biol. 2015;34:135-41 pubmed publisher
..These studies reveal that nuclear retention, diffusional mobility in the ER as well as the number and architecture of NPCs are major determinants of INM targeting, collectively lending support to a diffusion-retention-based mechanism. ..
Badertscher L, Wild T, Montellese C, Alexander L, Bammert L, Sarazova M, et al
. Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells. Cell Rep. 2015;13:2879-91 pubmed publisher
Kutay U, Hetzer M. Reorganization of the nuclear envelope during open mitosis. Curr Opin Cell Biol. 2008;20:669-77 pubmed publisher
..Here, we summarize recent progress in deciphering the molecular mechanisms underlying NE dynamics during cell division. ..
Wyler E, Wandrey F, Badertscher L, Montellese C, Alper D, Kutay U. The beta-isoform of the BRCA2 and CDKN1A(p21)-interacting protein (BCCIP) stabilizes nuclear RPL23/uL14. FEBS Lett. 2014;588:3685-91 pubmed publisher
..Overexpression of BCCIPÎ² leads to nucleoplasmic accumulation of extra-ribosomal RPL23 and stabilizes overexpressed RPL23, suggesting that BCCIPÎ² functions as nuclear chaperone for RPL23. ..
Ungricht R, Kutay U. A Wounded Nucleus Needs ESCRT Service. Dev Cell. 2016;37:202-4 pubmed publisher
..Nuclear envelope "wounds" are repaired by the ESCRT-III machinery to limit DNA damage, nuclear fragmentation, and cell death. ..
Bammert L, Jonas S, Ungricht R, Kutay U. Human AATF/Che-1 forms a nucleolar protein complex with NGDN and NOL10 required for 40S ribosomal subunit synthesis. Nucleic Acids Res. 2016;44:9803-9820 pubmed
..Our data help to explain the described role of AATF in cell proliferation during mouse development as well as its requirement for malignant tumor growth. ..
Wandrey F, Montellese C, Koos K, Badertscher L, Bammert L, Cook A, et al
. The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology. Mol Cell Biol. 2015;35:3491-503 pubmed publisher
..Together, these data indicate that NF45 and NF90 are novel higher-eukaryote-specific factors required for the maturation of 60S ribosomal subunits. ..
Kutay U, Hartmann E, Treichel N, Calado A, Carmo Fonseca M, Prehn S, et al
. Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily. J Biol Chem. 2000;275:40163-8 pubmed
..Like the other members of the transport receptor superfamily, RanBP16 interacts with the nuclear pore complex and is able to enter the nucleus independent of energy and additional nuclear transport receptors. ..