Peter Dimroth

Summary

Affiliation: Swiss Federal Institute of Technology
Country: Switzerland

Publications

  1. ncbi request reprint Molecular architecture of the undecameric rotor of a bacterial Na+-ATP synthase
    Janet Vonck
    Max Planck Institute of Biophysics, Heinrich Hoffmann Str 7, Frankfurt, Germany
    J Mol Biol 321:307-16. 2002
  2. ncbi request reprint Oxaloacetate decarboxylase of Archaeoglobus fulgidus: cloning of genes and expression in Escherichia coli
    Pius Dahinden
    Institut für Mikrobiologie der ETH Zürich, ETH Zentrum, Switzerland
    Arch Microbiol 182:414-20. 2004
  3. ncbi request reprint Identification of a domain in the alpha-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the gamma-subunit
    Pius Dahinden
    Institute of Microbiology ETH Zürich, ETH Honggerberg, Zurich, Switzerland
    FEBS J 272:846-55. 2005
  4. pmc Catalytic and mechanical cycles in F-ATP synthases. Fourth in the Cycles Review Series
    Peter Dimroth
    Institute of Microbiology, ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zürich Hönggerberg, Switzerland
    EMBO Rep 7:276-82. 2006
  5. pmc Inhibition of ATP hydrolysis by thermoalkaliphilic F1Fo-ATP synthase is controlled by the C terminus of the epsilon subunit
    Stefanie Keis
    Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, P O Box 56, Dunedin, New Zealand
    J Bacteriol 188:3796-804. 2006
  6. ncbi request reprint Energy conservation in the decarboxylation of dicarboxylic acids by fermenting bacteria
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Arch Microbiol 170:69-77. 1998
  7. ncbi request reprint Operation of the F(0) motor of the ATP synthase
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1458:374-86. 2000
  8. ncbi request reprint ATP synthesis by decarboxylation phosphorylation
    Peter Dimroth
    Institute of Microbiology, ETH Zurich, Wolfgang Pauli Strasse 10, 8093, Zurich, Switzerland
    Results Probl Cell Differ 45:153-84. 2008
  9. ncbi request reprint Coupling mechanism of the oxaloacetate decarboxylase Na(+) pump
    P Dimroth
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1505:1-14. 2001
  10. ncbi request reprint Enzymic and genetic basis for bacterial growth on malonate
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
    Mol Microbiol 25:3-10. 1997

Collaborators

Detail Information

Publications72

  1. ncbi request reprint Molecular architecture of the undecameric rotor of a bacterial Na+-ATP synthase
    Janet Vonck
    Max Planck Institute of Biophysics, Heinrich Hoffmann Str 7, Frankfurt, Germany
    J Mol Biol 321:307-16. 2002
    ..Three possible inter-subunit ion-bridges are likely to account for the remarkable temperature stability of I.tartaricus c-rings compared to those of other organisms...
  2. ncbi request reprint Oxaloacetate decarboxylase of Archaeoglobus fulgidus: cloning of genes and expression in Escherichia coli
    Pius Dahinden
    Institut für Mikrobiologie der ETH Zürich, ETH Zentrum, Switzerland
    Arch Microbiol 182:414-20. 2004
    ..Conversely, the carboxyl transferase domain of KpOadA exhibited catalytic activity not only with its pertinent biotin domain but also withAfOadC...
  3. ncbi request reprint Identification of a domain in the alpha-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the gamma-subunit
    Pius Dahinden
    Institute of Microbiology ETH Zürich, ETH Honggerberg, Zurich, Switzerland
    FEBS J 272:846-55. 2005
    ..This peptide therefore represents a newly identified, separate domain of the alpha-subunit and has been called the 'association domain'...
  4. pmc Catalytic and mechanical cycles in F-ATP synthases. Fourth in the Cycles Review Series
    Peter Dimroth
    Institute of Microbiology, ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zürich Hönggerberg, Switzerland
    EMBO Rep 7:276-82. 2006
    ....
  5. pmc Inhibition of ATP hydrolysis by thermoalkaliphilic F1Fo-ATP synthase is controlled by the C terminus of the epsilon subunit
    Stefanie Keis
    Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, P O Box 56, Dunedin, New Zealand
    J Bacteriol 188:3796-804. 2006
    ..Reconstituted TA2F(o)F(1)(epsilon(6A)) exhibited ATPase activity that correlated with increased ATP-driven proton pumping, confirming that the epsilon subunit also inhibits ATPase activity of TA2F(o)F(1)...
  6. ncbi request reprint Energy conservation in the decarboxylation of dicarboxylic acids by fermenting bacteria
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Arch Microbiol 170:69-77. 1998
    ..This new type of energy conservation has been termed decarboxylation phosphorylation and is responsible entirely for ATP synthesis in several anaerobic bacteria...
  7. ncbi request reprint Operation of the F(0) motor of the ATP synthase
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1458:374-86. 2000
    ..Taken together with biophysical data, the operating principles of the motor have been delineated...
  8. ncbi request reprint ATP synthesis by decarboxylation phosphorylation
    Peter Dimroth
    Institute of Microbiology, ETH Zurich, Wolfgang Pauli Strasse 10, 8093, Zurich, Switzerland
    Results Probl Cell Differ 45:153-84. 2008
    ..In the second part, we provide a detailed description of the F(1)F(0) ATP synthases with special emphasis on the Na(+)-translocating variant of these enzymes...
  9. ncbi request reprint Coupling mechanism of the oxaloacetate decarboxylase Na(+) pump
    P Dimroth
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1505:1-14. 2001
    ..With H(+) entering from the periplasm, the hydroxyl group of S382 is regenerated, and as a consequence, the Na(+) ions are released into this compartment. After switching back to the original conformation, Na(+) pumping continues...
  10. ncbi request reprint Enzymic and genetic basis for bacterial growth on malonate
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
    Mol Microbiol 25:3-10. 1997
    ..The genetic evidence firmly supports the idea that malonate decarboxylation is carried out by the two different decarboxylases, as deduced from the biochemical studies of the enzymes...
  11. ncbi request reprint Electrical power fuels rotary ATP synthase
    Peter Dimroth
    Institut für Mikrobiologie der Eidgenössischen Technischen Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    Structure 11:1469-73. 2003
    ..Here, we incorporate recent results on structure and function of the F(0) domain and present a mechanism for torque generation with the fundamental nature of the membrane potential as driving force in the core...
  12. ncbi request reprint Crucial role of the membrane potential for ATP synthesis by F(1)F(o) ATP synthases
    P Dimroth
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zentrum, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    J Exp Biol 203:51-9. 2000
    ..These conclusions are corroborated by the finding that only the membrane potential induces a rotary torque that drives the counter-rotation of the a and c subunits in the F(o) motor of Propionigenium modestum ATP synthase...
  13. ncbi request reprint Arginine-induced conformational change in the c-ring/a-subunit interface of ATP synthase
    Thomas Vorburger
    Institut fur Mikrobiologie, ETH Zurich Honggerberg, Switzerland
    FEBS J 275:2137-50. 2008
    ..This mechanism allows efficient interaction between subunit a and the c-ring and simultaneously allows almost frictionless movement against each other...
  14. ncbi request reprint The structural basis for unidirectional rotation of thermoalkaliphilic F1-ATPase
    Achim Stocker
    Institute of Microbiology ETH Zürich, ETH Honggerberg, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
    Structure 15:904-14. 2007
    ..A similar bending of the gamma shaft as in the TA2F(1) structure was observed by single-particle analysis of the TA2F(1)F(o) holoenzyme...
  15. ncbi request reprint Two distinct proton binding sites in the ATP synthase family
    Christoph von Ballmoos
    Institut fur Mikrobiologie, ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
    Biochemistry 46:11800-9. 2007
    ..The concept of two different binding sites in the ATP synthase family is supported by the ATP hydrolysis pH profiles of the investigated enzymes...
  16. pmc The citrate carrier CitS probed by single-molecule fluorescence spectroscopy
    Christopher N Kästner
    Eidgenossische Technische Hochschule Zurich, Institut fur Mikrobiologie, Institut fur Physikalische Chemie, Switzerland
    Biophys J 84:1651-9. 2003
    ..Single-molecule studies with dual-labeled CitS-sC398-AF(546/568) and dual-color detection provided strong evidence for a homodimeric association of CitS...
  17. doi request reprint Functional asymmetry of the F(0) motor in bacterial ATP synthases
    Alexander Wiedenmann
    Institut fur Mikrobiologie, ETH Zurich, Zurich, Switzerland
    Mol Microbiol 72:479-90. 2009
    ..5 were required for ATP synthesis in vitro. This discrepancy is discussed with respect to the hypothesis that during respiration lateral proton diffusion could lead to significant acidification at the membrane surface...
  18. ncbi request reprint Deltapsi and DeltapH are equivalent driving forces for proton transport through isolated F(0) complexes of ATP synthases
    Alexander Wiedenmann
    Institut fur Mikrobiologie, ETH Zurich, Zurich, Switzerland
    Biochim Biophys Acta 1777:1301-10. 2008
    ..The new method is an ideal tool for detailed kinetic investigations of the ion transport mechanism of ATP synthases from various organisms...
  19. ncbi request reprint Structure of the rotor ring of F-Type Na+-ATPase from Ilyobacter tartaricus
    Thomas Meier
    Institut fur Mikrobiologie, Eidgenössische Technische Hochschule ETH, Zürich Hönggerberg, Wolfgang Pauli Str 10, CH 8093 Zurich, Switzerland
    Science 308:659-62. 2005
    ....
  20. pmc Structure-function relations in oxaloacetate decarboxylase complex. Fluorescence and infrared approaches to monitor oxomalonate and Na(+) binding effect
    Thierry Granjon
    Institute of Microbiology, Eidgenossische Technische Hochschule, Zurich, Switzerland
    PLoS ONE 5:e10935. 2010
    ..OAD is a membrane-bound enzyme composed of alpha, beta and gamma subunits. The alpha subunit contains the carboxyltransferase catalytic site...
  21. ncbi request reprint Molecular mechanism of the ATP synthase's F(o) motor probed by mutational analyses of subunit a
    Franziska Wehrle
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    J Mol Biol 322:369-81. 2002
    ....
  22. ncbi request reprint Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases
    Christoph von Ballmoos
    Institut für Mikrobiologie der Eidgenössischen Technischen Hochschule, ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 269:5581-9. 2002
    ..The common membrane embedded location of the binding site of ATP synthases suggest a common mechanism for ion transfer across the membrane...
  23. ncbi request reprint Na(+)-coupled alternative to H(+)-coupled primary transport systems in bacteria
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
    Bioessays 13:463-8. 1991
    ....
  24. ncbi request reprint Evidence for structural integrity in the undecameric c-rings isolated from sodium ATP synthases
    Thomas Meier
    ETH Zentrum, Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    J Mol Biol 325:389-97. 2003
    ....
  25. ncbi request reprint Site-directed sulfhydryl labeling of the oxaloacetate decarboxylase Na+ pump of Klebsiella pneumoniae: helix VIII comprises a portion of the sodium ion channel
    Markus R Wild
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    Biochemistry 42:11615-24. 2003
    ..The betaR389C mutant had a reduced activity and a pH optimum at pH 9, which could be restored to a wild-type pH optimum of 6.5 and to a 400% gain in activity upon chemical modification with 2-aminoethyl methanethiosulfonate...
  26. pmc The ion channel of F-ATP synthase is the target of toxic organotin compounds
    Christoph von Ballmoos
    Institut für Mikrobiologie der Eidgenössischen Technischen Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    Proc Natl Acad Sci U S A 101:11239-44. 2004
    ..Taken together these results indicate that the subunit a ion channel is the target site for ATPase inhibition by toxic organotin compounds. An inhibitor interacting specifically with this site has not been reported previously...
  27. ncbi request reprint Phospholipids occupy the internal lumen of the c ring of the ATP synthase of Escherichia coli
    Benjamin Oberfeld
    Institut fur Mikrobiologie, ETH Zurich, 8093 Zurich, Switzerland
    Biochemistry 45:1841-51. 2006
    ..We conclude therefore that the inner lumen of the c ring is occupied with phospholipids. No evidence was found for an extension of subunit a into this space...
  28. ncbi request reprint Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase
    Thomas Meier
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Zurich, Switzerland
    FEBS J 272:5474-83. 2005
    ..Our results are congruent with a stable c11 ring stoichiometry that seems to not be influenced by the expression level of subunit c in the bacteria...
  29. pmc Sodium ion cycling mediates energy coupling between complex I and ATP synthase
    Anja C Gemperli
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    Proc Natl Acad Sci U S A 100:839-44. 2003
    ..No NADH-driven proton transport into the lumen of proteoliposomes was detected. We conclude that Na(+) is used as the exclusive coupling ion by the enterobacterial complex I...
  30. ncbi request reprint Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase beta subunit in the energy coupling mechanism of the Na+ pump
    Markus Schmid
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 269:2997-3004. 2002
    ..During the countertransport of 2 Na+ and 1 H+ Y229 of center II switches between the protonated and deprotonated Na+-bound state...
  31. ncbi request reprint Characterization of the citrate/acetate antiporter CitW of Klebsiella pneumoniae
    Christopher N Kästner
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland
    Arch Microbiol 177:500-6. 2002
    ..A.24). The substrate specificity seems to partially contradict this phylogenetic classification, but appears logical with respect to the putative functional role of CitW in the citrate fermentation pathway of K. pneumoniae...
  32. ncbi request reprint The respiratory complex I (NDH I) from Klebsiella pneumoniae, a sodium pump
    Anja C Gemperli
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    J Biol Chem 277:33811-7. 2002
    ..6 units mg(-1), 2.4 units mg(-1), and 4.7 units mg(-1), respectively, which indicate a Na(+)/electron stoichiometry of one...
  33. ncbi request reprint Purification, crystallization, and properties of F1-ATPase complexes from the thermoalkaliphilic Bacillus sp. strain TA2.A1
    Achim Stocker
    Institute of Microbiology ETH Zürich, ETH Honggerberg, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
    J Struct Biol 152:140-5. 2005
    ..A1 membranes. ATPase assays of F(1)Deltadelta crystals exhibited latent ATP hydrolytic activity that was highly stimulated by lauryldimethylamine oxide, a hallmark of the native enzyme...
  34. doi request reprint Probing the rotor subunit interface of the ATP synthase from Ilyobacter tartaricus
    Denys Pogoryelov
    Institute of Microbiology, Eidgenossische Technische Hochschule, Zurich, Switzerland
    FEBS J 275:4850-62. 2008
    ....
  35. ncbi request reprint In vitro binding of the response regulator CitB and of its carboxy-terminal domain to A + T-rich DNA target sequences in the control region of the divergent citC and citS operons of Klebsiella pneumoniae
    M Meyer
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Zurich, Switzerland
    J Mol Biol 269:719-31. 1997
    ..The positions of the binding sites for CitB and of putative recognition sequences for the cAMP receptor protein suggested a model for the interaction of these activators with RNA polymerase...
  36. ncbi request reprint A continuous fluorescent method for measuring Na+ transport
    Christoph von Ballmoos
    Institut für Mikrobiologie der Eidgenössischen Technischen Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    Anal Biochem 335:334-7. 2004
  37. ncbi request reprint Coupled rotation within single F0F1 enzyme complexes during ATP synthesis or hydrolysis
    Georg Kaim
    Institute of Microbiology, ETH Zurich, Schmelzbergstrasse 7, CH 8092, Zurich, Switzerland
    FEBS Lett 525:156-63. 2002
    ..Moreover, upon the incubation with the F0-specific inhibitor dicyclohexylcarbodiimide the rotation was severely inhibited...
  38. ncbi request reprint Membrane topography of the coupling ion binding site in Na+-translocating F1F0 ATP synthase
    Christoph von Ballmoos
    Institut für Mikrobiologie der Eidgenössischen Technischen Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    J Biol Chem 277:3504-10. 2002
    ..The substitutions were found exclusively at the fatty acid side chains, which indicates that c-Glu(65) is located within the core of the membrane...
  39. pmc Intersubunit bridging by Na+ ions as a rationale for the unusual stability of the c-rings of Na+-translocating F1F0 ATP synthases
    Thomas Meier
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    EMBO Rep 3:1094-8. 2002
    ..Site-directed mutagenesis strengthens the essential role of glutamate 65 in the crossbridging of the subunits and also corroborates the proposed stabilizing effect of an ion bridge including aspartate 2...
  40. ncbi request reprint Reconstitution of Fo of the sodium ion translocating ATP synthase of Propionigenium modestum from its heterologously expressed and purified subunits
    Franziska Wehrle
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Zurich, Switzerland
    Eur J Biochem 269:2567-73. 2002
    ..modestum and c(11) from Ilyobacter tartaricus. These Fo hybrids had Na(+) translocation activities that were not distinguishable from that of P. modestum F(o)...
  41. ncbi request reprint Oxaloacetate decarboxylase of Vibrio cholerae: purification, characterization, and expression of the genes in Escherichia coli
    Pius Dahinden
    Institut für Mikrobiologie der ETH Zürich, ETH Honggerberg, Wolfgang Pauli Strasse 10, 8093 Zurich, Switzerland
    Arch Microbiol 183:121-9. 2005
    ..The two oad gene clusters were heterologously expressed in Escherichia coli, and the decarboxylases were isolated from the host cells...
  42. ncbi request reprint Subunit gamma of the oxaloacetate decarboxylase Na(+) pump: interaction with other subunits/domains of the complex and binding site for the Zn(2+) metal ion
    Markus Schmid
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland
    Biochemistry 41:1285-92. 2002
    ..These results indicate that aspartate 62, histidine 77, and histidine 82 of the gamma subunit are ligands for the catalytically important Zn(2+) metal ion...
  43. pmc The c15 ring of the Spirulina platensis F-ATP synthase: F1/F0 symmetry mismatch is not obligatory
    Denys Pogoryelov
    Institute of Microbiology, Eidgenossische Technische Hochschule, Wolfgang Pauli Strasse 10, Zurich 8093, Switzerland
    EMBO Rep 6:1040-4. 2005
    ..platensis showed 15 symmetrically assembled subunits. The c15-mer reported here is the largest c ring that is isolated and does not show the classical c-ring mismatch to the three-fold symmetry of the F1 domain...
  44. ncbi request reprint Unique rotary ATP synthase and its biological diversity
    Christoph von Ballmoos
    Institut fur Mikrobiologie, ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
    Annu Rev Biophys 37:43-64. 2008
    ....
  45. ncbi request reprint Bacterial Na+ - or H+ -coupled ATP synthases operating at low electrochemical potential
    Peter Dimroth
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zentrum, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    Adv Microb Physiol 49:175-218. 2004
    ....
  46. pmc The Escherichia coli citrate carrier CitT: a member of a novel eubacterial transporter family related to the 2-oxoglutarate/malate translocator from spinach chloroplasts
    K M Pos
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule Zurich, CH 8092 Zurich, Switzerland
    J Bacteriol 180:4160-5. 1998
    ..It is suggested that the E. coli CitT protein is a member of a novel family of eubacterial transporters involved in the transport of di- and tricarboxylic acids...
  47. pmc Catabolite repression of the citrate fermentation genes in Klebsiella pneumoniae: evidence for involvement of the cyclic AMP receptor protein
    M Meyer
    , , , Switzerland
    J Bacteriol 183:5248-56. 2001
    ..These data indicate that catabolite repression of the citrate fermentation genes is exerted by CRP and that in the absence of repressing carbon sources the cAMP-CRP complex serves to enhance the basal, CitB-dependent transcription level...
  48. pmc Identification of a gene cluster in Klebsiella pneumoniae which includes citX, a gene required for biosynthesis of the citrate lyase prosthetic group
    Karin Schneider
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule Zurich, CH 8092 Zurich, Switzerland
    J Bacteriol 184:2439-46. 2002
    ..Similar to the other citrate fermentation enzymes, CitX synthesis was apparently subject to catabolite repression...
  49. ncbi request reprint Sequence of the sodium ion pump oxaloacetate decarboxylase from Salmonella typhimurium
    G Woehlke
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
    J Biol Chem 267:22798-803. 1992
    ..A plasmid encoding the three oad genes and that for the anaerobic citrate carrier (citS) was cloned from the chromosomal DNA and used for sequence determination...
  50. ncbi request reprint Sequence of a gene cluster from Klebsiella pneumoniae encoding malonate decarboxylase and expression of the enzyme in Escherichia coli
    S Hoenke
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, Zurich, Switzerland
    Eur J Biochem 246:530-8. 1997
    ..As partial deletion of the mdcR gene impaired growth of the transformants on malonate, MdcR is probably a transcriptional regulator of the mdc genes...
  51. ncbi request reprint Critical evaluation of the one- versus the two-channel model for the operation of the ATP synthase's F(o) motor
    P Dimroth
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zentrum, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1459:506-13. 2000
    ....
  52. ncbi request reprint Regulation of anaerobic citrate metabolism in Klebsiella pneumoniae
    M Bott
    Mikrobiologisches Institut, Eidgenoessische Technische Hochschule, Zurich, Switzerland
    Mol Microbiol 18:533-46. 1995
    ..In the presence of these signals, the citAB gene products induced their own synthesis. The positive autoregulation occurred via co-transcription of citAB with citS and oadGAB...
  53. ncbi request reprint NMR investigations of subunit c of the ATP synthase from Propionigenium modestum in chloroform/methanol/water (4 : 4 : 1)
    Ulrich Matthey
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Zurich, Switzerland
    Eur J Biochem 269:1942-6. 2002
    ..modestum in the organic solvent also deviated from that of Escherichia coli in the same environment, the most important difference being that subunit c of P. modestum did not adopt a stable hairpin structure like subunit c of E. coli...
  54. ncbi request reprint Biosynthesis of the prosthetic group of citrate lyase
    K Schneider
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule Zurich, Switzerland
    Biochemistry 39:9438-50. 2000
    ..Second, holo-ACP is formed from apo-ACP and the prosthetic group precursor in a reaction catalyzed by CitX...
  55. ncbi request reprint Klebsiella pneumoniae genes for citrate lyase and citrate lyase ligase: localization, sequencing, and expression
    M Bott
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Zurich, Switzerland
    Mol Microbiol 14:347-56. 1994
    ..These and other data strongly indicated that the enzyme synthesized in E. coli lacked its prosthetic group. Thus, additional genes besides citCDEFG appear to be required for the formation of holo-citrate lyase...
  56. ncbi request reprint On the mechanism of sodium ion translocation by oxaloacetate decarboxylase of Klebsiella pneumoniae
    P Dimroth
    Mikrobiologisches Institut, Eidgenössischen Technischen Hochschule, Zurich, Switzerland
    Biochemistry 32:1734-9. 1993
    ..A model of Na+ translocation by oxaloacetate decarboxylase based on these experimental results is proposed...
  57. ncbi request reprint Osmomechanics of the Propionigenium modestum F(o) motor
    P Dimroth
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, ETH Zentrum, CH 8092 Zurich, Switzerland micro biol ethz ch
    J Bioenerg Biomembr 32:449-58. 2000
    ..We propose a catalytic mechanism for torque generation by the F(o) motor that is in accord with all experimental data and is in quantitative agreement with the requirement for ATP synthesis...
  58. ncbi request reprint Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Mol Microbiol 35:428-34. 2000
    ..With an E. coli mutant deficient in complex I, the Na+ transport activity was low (1-3 nmol mg-1 min-1), and rotenone was without effect...
  59. ncbi request reprint The Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus--redox states of the FAD prosthetic group and mechanism of Ag+ inhibition
    J Steuber
    ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 249:770-6. 1997
    ..The increase in fluorescence intensity correlated with the loss of enzyme activity. Gel filtration of the Ag+-treated Na+-NQR confirmed that FAD had been displaced from the holo-enzyme...
  60. ncbi request reprint Inactivation of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus by reactive oxygen species
    Julia Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Zurich, Switzerland
    Eur J Biochem 269:1287-92. 2002
    ..alginolyticus. It is shown that the [2Fe-2S] cluster previously assigned to the Na+-NQR originates from the succinate dehydrogenase or the related enzyme fumarate reductase...
  61. ncbi request reprint Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group
    K Schneider
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule Zurich, Switzerland
    FEBS Lett 483:165-8. 2000
    ..Furthermore, this precursor was transferred by CitX to apo-ACP, yielding holo-ACP. Thus, our proposed mechanism for holo-ACP synthesis could be verified...
  62. ncbi request reprint Structure and function of the Na(+)-translocating ATPase of Propionigenium modestum
    P Dimroth
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Zurich, Switzerland
    Acta Physiol Scand Suppl 607:97-103. 1992
    ..The phylogenetic relationship between the Na(+)-translocating ATPase of P. modestum and other F1F0 ATPases has been clearly demonstrated by sequencing studies...
  63. ncbi request reprint The central plug in the reconstituted undecameric c cylinder of a bacterial ATP synthase consists of phospholipids
    T Meier
    , , ETH-Zentrum, , Switzerland
    FEBS Lett 505:353-6. 2001
    ..As the detergent-purified c cylinder is completely devoid of phospholipids, these are incorporated into the central hole from one side of the cylinder during the reconstitution procedure...
  64. pmc Torque generation by the Fo motor of the sodium ATPase
    Jianhua Xing
    Department of Molecular Biology, Policy and Management, University of California, Berkeley, California 94720 3112, USA
    Biophys J 87:2148-63. 2004
    ..This work also illustrates how to extract a motor mechanism from dynamical experimental observations in the absence of complete structural information...
  65. ncbi request reprint A specific adaptation in the a subunit of thermoalkaliphilic F1FO-ATP synthase enables ATP synthesis at high pH but not at neutral pH values
    Duncan G G McMillan
    Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand
    J Biol Chem 282:17395-404. 2007
    ....
  66. pmc Structural investigations of the membrane-embedded rotor ring of the F-ATPase from Clostridium paradoxum
    Thomas Meier
    Max Planck Institute of Biophysics, Max von Laue Strasse 3, D 60438 Frankfurt, Germany
    J Bacteriol 188:7759-64. 2006
    ..On the basis of these results, it was determined that the F-ATPase from C. paradoxum contains an undecameric c ring...
  67. ncbi request reprint A tridecameric c ring of the adenosine triphosphate (ATP) synthase from the thermoalkaliphilic Bacillus sp. strain TA2.A1 facilitates ATP synthesis at low electrochemical proton potential
    Thomas Meier
    Max Planck Institute of Biophysics, Max von Laue Str 3, 60438 Frankfurt am Main, Germany
    Mol Microbiol 65:1181-92. 2007
    ..The operation of a c ring with 13 subunits renders the thermodynamic problem of ATP synthesis at alkaline pH less severe and may represent a strategy for ATP synthesis at low electrochemical potential...
  68. pmc Bioenergetic properties of the thermoalkaliphilic Bacillus sp. strain TA2.A1
    Karen Olsson
    Department of Microbiology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand
    J Bacteriol 185:461-5. 2003
    ..A1 correlated well with the DeltapNa(+), phosphorylation potential, and the ATP/ADP ratio, but not with Deltap. This communication represents the first report on the bioenergetics of an extremely thermoalkaliphilic aerobic bacterium...
  69. pmc Charge displacements during ATP-hydrolysis and synthesis of the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus
    Christiane Burzik
    Max Planck Institut fur Biophysik, D 60596 Frankfurt Main, Germany
    Biophys J 85:2044-54. 2003
    ..For ATP synthesis the rate constant is 50-70 s(-1)...
  70. pmc Purification and biochemical characterization of the F1Fo-ATP synthase from thermoalkaliphilic Bacillus sp. strain TA2.A1
    Gregory M Cook
    Department of Microbiology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand
    J Bacteriol 185:4442-9. 2003
    ..A1, as documented previously for mesophilic alkaliphilic Bacillus species. The ATP synthase was specifically modified at its c subunits by N,N'-dicyclohexylcarbodiimide, and this modification inhibited ATP synthesis...
  71. pmc The oligomeric state of c rings from cyanobacterial F-ATP synthases varies from 13 to 15
    Denys Pogoryelov
    Max Planck Institute of Biophysics, Max von Laue Strasse 3, 60438 Frankfurt am Main, Germany
    J Bacteriol 189:5895-902. 2007
    ..Furthermore, the two new examples of c(15) rings underline once more that an F(1)/F(o) symmetry mismatch is not an obligatory feature of all F-ATP synthases...
  72. ncbi request reprint Observing membrane protein diffusion at subnanometer resolution
    Daniel J Muller
    Max Planck Institute of Molecular Cell Biology and Genetics, Biotechnology Center, Pfotenhauerstr 108, 01307, Dresden, Germany
    J Mol Biol 327:925-30. 2003
    ....