Kai Johnsson

Summary

Country: Switzerland

Publications

  1. doi request reprint Visualizing biochemical activities in living cells
    Kai Johnsson
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Nat Chem Biol 5:63-5. 2009
  2. ncbi request reprint Fast directed evolution of non-immunoglobulin proteins by somatic hypermutation in immune cells
    Christian Heinis
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Chembiochem 6:804-6. 2005
  3. pmc Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)
    Matthias Geissbuehler
    Laboratoire d Optique Biomedicale, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Biophys J 98:339-49. 2010
  4. ncbi request reprint Evolving the substrate specificity of O6-alkylguanine-DNA alkyltransferase through loop insertion for applications in molecular imaging
    Christian Heinis
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, CH 1015 Lausanne, Switzerland
    ACS Chem Biol 1:575-84. 2006
  5. doi request reprint Using peptide loop insertion mutagenesis for the evolution of proteins
    Christian Heinis
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de, Lausanne EPFL, Lausanne, Switzerland
    Methods Mol Biol 634:217-32. 2010
  6. ncbi request reprint Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution
    Petra Tafelmeyer
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 11:681-9. 2004
  7. ncbi request reprint Chemical probes shed light on protein function
    Kai Johnsson
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Curr Opin Struct Biol 17:488-94. 2007
  8. doi request reprint Selective cross-linking of interacting proteins using self-labeling tags
    Arnaud Gautier
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 131:17954-62. 2009
  9. doi request reprint Fura-2FF-based calcium indicator for protein labeling
    Agostina A Ruggiu
    Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Org Biomol Chem 8:3398-401. 2010
  10. doi request reprint Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore
    Mako Kamiya
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    Anal Chem 82:6472-9. 2010

Collaborators

  • Christian Heinis
  • Eiji Nakata
  • Dimitri van de Ville
  • Boris Hinz
  • Marlon J Hinner
  • Matthias Geissbuehler
  • Arnaud Gautier
  • Maik Kindermann
  • Michael Bannwarth
  • Sambashiva Banala
  • Alexandre Juillerat
  • India Sielaff
  • Agostina A Ruggiu
  • Damien Maurel
  • Mako Kamiya
  • Kui Thong Tan
  • Matthias A Brun
  • Christopher Chidley
  • Anke Arnold
  • Stefano Tugulu
  • Laura Vivero-Pol
  • Nils Johnsson
  • Antje Keppler
  • Petra Tafelmeyer
  • Thierry Laroche
  • Leandro Royer
  • Eduardo Rois
  • Ivan R Correa
  • Annina Aebischer
  • Cindy Fellay
  • Sandrine Pouvreau
  • Grazvydas Lukinavicius
  • Monika Sztretye
  • Florent Beaufils
  • Katarzyna Mosiewicz
  • Ivan Reis Corrêa
  • Nathalie George
  • Alexey Terskikh
  • Jan Barnikow
  • Holger Krumm
  • Harm Anton Klok
  • Hugues Jaccard
  • Beatrice Kunz
  • Horst Vogel
  • Susanne Gendreizig
  • Horst Pick

Detail Information

Publications21

  1. doi request reprint Visualizing biochemical activities in living cells
    Kai Johnsson
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Nat Chem Biol 5:63-5. 2009
    ..Here I highlight some of the areas where the development of new probes and labeling methods is eagerly awaited and where chemical biologists could make important contributions...
  2. ncbi request reprint Fast directed evolution of non-immunoglobulin proteins by somatic hypermutation in immune cells
    Christian Heinis
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Chembiochem 6:804-6. 2005
  3. pmc Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)
    Matthias Geissbuehler
    Laboratoire d Optique Biomedicale, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Biophys J 98:339-49. 2010
    ..The proposed method has the potential to become a new tool for investigating oxygen metabolism at the single cell and the subcellular level...
  4. ncbi request reprint Evolving the substrate specificity of O6-alkylguanine-DNA alkyltransferase through loop insertion for applications in molecular imaging
    Christian Heinis
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, CH 1015 Lausanne, Switzerland
    ACS Chem Biol 1:575-84. 2006
    ..This allowed the establishment of fluorescence-based assays to detect protein-protein interactions and measure enzymatic activities...
  5. doi request reprint Using peptide loop insertion mutagenesis for the evolution of proteins
    Christian Heinis
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de, Lausanne EPFL, Lausanne, Switzerland
    Methods Mol Biol 634:217-32. 2010
    ..The experimental procedures are general and can easily be adapted for the insertion of peptide loops into other classes of enzymes or into any protein of interest...
  6. ncbi request reprint Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution
    Petra Tafelmeyer
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 11:681-9. 2004
    ..This powerful selection complements the repertoire of the currently used split-protein sensors and provides a new tool for high-throughput interaction screening...
  7. ncbi request reprint Chemical probes shed light on protein function
    Kai Johnsson
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Curr Opin Struct Biol 17:488-94. 2007
    ..The past two years have seen significant advances in such methods, the publication of a number of new tags for labeling, and the imaginative application of established techniques to tackle previously intractable biological questions...
  8. doi request reprint Selective cross-linking of interacting proteins using self-labeling tags
    Arnaud Gautier
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 131:17954-62. 2009
    ....
  9. doi request reprint Fura-2FF-based calcium indicator for protein labeling
    Agostina A Ruggiu
    Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Org Biomol Chem 8:3398-401. 2010
    ..We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein...
  10. doi request reprint Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore
    Mako Kamiya
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    Anal Chem 82:6472-9. 2010
    ..The high sensitivity of the indicator together with the possibility to selectively couple it to proteins of interest makes it a powerful tool for measuring local changes in Ca2+ concentrations in living cells...
  11. doi request reprint Semisynthetic fluorescent sensor proteins based on self-labeling protein tags
    Matthias A Brun
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    J Am Chem Soc 131:5873-84. 2009
    ..These features should allow semisynthetic fluorescent sensor proteins based on SNAP-tag to become important tools for probing previously inaccessible metabolites...
  12. pmc Indo-1 derivatives for local calcium sensing
    Michael Bannwarth
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    ACS Chem Biol 4:179-190. 2009
    ....
  13. doi request reprint Photoactivatable and photoconvertible fluorescent probes for protein labeling
    Damien Maurel
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, Switzerland
    ACS Chem Biol 5:507-16. 2010
    ..This work establishes a generally applicable strategy for the generation of a large variety of different photosensitive fluorophores with tailor-made properties for biomolecular imaging...
  14. doi request reprint A designed protein for the specific and covalent heteroconjugation of biomolecules
    Christopher Chidley
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, CH 1015 Lausanne, Switzerland
    Bioconjug Chem 19:1753-6. 2008
    ..The efficiency and specificity of covalin-based bioconjugations together with the availability of a large variety of substrates create immediate and ubiquitous applications for covalin in bioconjugate chemistry...
  15. doi request reprint An engineered protein tag for multiprotein labeling in living cells
    Arnaud Gautier
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 15:128-36. 2008
    ..We furthermore show simultaneous pulse-chase experiments to visualize different generations of two different proteins in one sample...
  16. ncbi request reprint Caged substrates for protein labeling and immobilization
    Sambashiva Banala
    Ecole Polytechnique Federal de Lausanne EPFL, Institute of Chemical Sciences and Engineering, 1015 Lausanne, Switzerland
    Chembiochem 9:38-41. 2008
  17. ncbi request reprint Multicolor imaging of cell surface proteins
    Laura Vivero-Pol
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 127:12770-1. 2005
    ..In another application, fusions to different CPs can be selectively labeled with different fluorophores in one sample. Both applications open up new ways for studying the properties of cell surface proteins of living cells...
  18. ncbi request reprint Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells
    Alexandre Juillerat
    Ecole Polytechnique Federale de Lausanne, Institute of Chemical Sciences and Engineering, 1015 Lausanne, Switzerland
    Chembiochem 6:1263-9. 2005
    ....
  19. ncbi request reprint Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins
    Maik Kindermann
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    Bioorg Med Chem Lett 14:2725-8. 2004
    ..These molecules should become useful tools for various applications in functional proteomics...
  20. ncbi request reprint Protein-functionalized polymer brushes
    Stefano Tugulu
    Laboratoire des Polymères, Institut des materiaux, Bâtiment MX D, and Laboratoire d Ingénierie des Protéines, Institut des Sciences et Ingenierie Chimiques, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Biomacromolecules 6:1602-7. 2005
    ..These protein-modified polymer brushes are attractive candidates for the development of protein microarrays...
  21. ncbi request reprint Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Ecole Polytechnique Federale de Lausanne, Lausanne CH 1015, Switzerland
    Methods 32:437-44. 2004
    ..Here, we report optimized protocols for the synthesis of O6-benzylguanine derivatives and the use of such derivatives for the labeling of different hAGT fusion proteins in vivo and in vitro...