Kai Johnsson

Summary

Country: Switzerland

Publications

  1. pmc Exploiting ligand-protein conjugates to monitor ligand-receptor interactions
    Hirohito Haruki
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR in Chemical Biology, Lausanne, Switzerland
    PLoS ONE 7:e37598. 2012
  2. doi request reprint Visualizing biochemical activities in living cells
    Kai Johnsson
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Nat Chem Biol 5:63-5. 2009
  3. ncbi request reprint Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Ecole Polytechnique Federale de Lausanne, Lausanne CH 1015, Switzerland
    Methods 32:437-44. 2004
  4. ncbi request reprint Covalent labeling of cell-surface proteins for in-vivo FRET studies
    Bruno H Meyer
    Ecole Polytechnique Federale de Lausanne EPFL, Institut de Science et Ingénierie Chimiques, CH 1015 Lausanne, Switzerland
    FEBS Lett 580:1654-8. 2006
  5. ncbi request reprint Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling
    Thomas Gronemeyer
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering CH 1015 Lausanne, Switzerland
    Protein Eng Des Sel 19:309-16. 2006
  6. doi request reprint An engineered protein tag for multiprotein labeling in living cells
    Arnaud Gautier
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 15:128-36. 2008
  7. ncbi request reprint Evolving the substrate specificity of O6-alkylguanine-DNA alkyltransferase through loop insertion for applications in molecular imaging
    Christian Heinis
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, CH 1015 Lausanne, Switzerland
    ACS Chem Biol 1:575-84. 2006
  8. doi request reprint Photoactivatable and photoconvertible fluorescent probes for protein labeling
    Damien Maurel
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, Switzerland
    ACS Chem Biol 5:507-16. 2010
  9. ncbi request reprint Protein function microarrays based on self-immobilizing and self-labeling fusion proteins
    India Sielaff
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, 1015 Lausanne, Switzerland
    Chembiochem 7:194-202. 2006
  10. doi request reprint A semisynthetic fluorescent sensor protein for glutamate
    Matthias A Brun
    Institute of Chemical Sciences and Engineering, National Centre of Competence in Research Chemical Biology, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Am Chem Soc 134:7676-8. 2012

Collaborators

Detail Information

Publications62

  1. pmc Exploiting ligand-protein conjugates to monitor ligand-receptor interactions
    Hirohito Haruki
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR in Chemical Biology, Lausanne, Switzerland
    PLoS ONE 7:e37598. 2012
    ..The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins...
  2. doi request reprint Visualizing biochemical activities in living cells
    Kai Johnsson
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Nat Chem Biol 5:63-5. 2009
    ..Here I highlight some of the areas where the development of new probes and labeling methods is eagerly awaited and where chemical biologists could make important contributions...
  3. ncbi request reprint Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Ecole Polytechnique Federale de Lausanne, Lausanne CH 1015, Switzerland
    Methods 32:437-44. 2004
    ..Here, we report optimized protocols for the synthesis of O6-benzylguanine derivatives and the use of such derivatives for the labeling of different hAGT fusion proteins in vivo and in vitro...
  4. ncbi request reprint Covalent labeling of cell-surface proteins for in-vivo FRET studies
    Bruno H Meyer
    Ecole Polytechnique Federale de Lausanne EPFL, Institut de Science et Ingénierie Chimiques, CH 1015 Lausanne, Switzerland
    FEBS Lett 580:1654-8. 2006
    ..FRET revealed interactions between cell-surface neurokinin-1 receptors simultaneously labeled with a controlled ratio of donors and acceptors. Moreover, using FRET the specific binding of fluorescent agonists could be monitored...
  5. ncbi request reprint Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling
    Thomas Gronemeyer
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering CH 1015 Lausanne, Switzerland
    Protein Eng Des Sel 19:309-16. 2006
    ..The results furthermore identify two regions of the protein outside the active site that influence the activity of the protein towards BG derivatives...
  6. doi request reprint An engineered protein tag for multiprotein labeling in living cells
    Arnaud Gautier
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 15:128-36. 2008
    ..We furthermore show simultaneous pulse-chase experiments to visualize different generations of two different proteins in one sample...
  7. ncbi request reprint Evolving the substrate specificity of O6-alkylguanine-DNA alkyltransferase through loop insertion for applications in molecular imaging
    Christian Heinis
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, CH 1015 Lausanne, Switzerland
    ACS Chem Biol 1:575-84. 2006
    ..This allowed the establishment of fluorescence-based assays to detect protein-protein interactions and measure enzymatic activities...
  8. doi request reprint Photoactivatable and photoconvertible fluorescent probes for protein labeling
    Damien Maurel
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, Switzerland
    ACS Chem Biol 5:507-16. 2010
    ..This work establishes a generally applicable strategy for the generation of a large variety of different photosensitive fluorophores with tailor-made properties for biomolecular imaging...
  9. ncbi request reprint Protein function microarrays based on self-immobilizing and self-labeling fusion proteins
    India Sielaff
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, 1015 Lausanne, Switzerland
    Chembiochem 7:194-202. 2006
    ..coli by different phosphopantetheine transferases (PPTases), yielding insights into the role of selected ACP amino acids in the ACP-PPTase interaction...
  10. doi request reprint A semisynthetic fluorescent sensor protein for glutamate
    Matthias A Brun
    Institute of Chemical Sciences and Engineering, National Centre of Competence in Research Chemical Biology, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Am Chem Soc 134:7676-8. 2012
    ..56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology...
  11. doi request reprint Selective cross-linking of interacting proteins using self-labeling tags
    Arnaud Gautier
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 131:17954-62. 2009
    ....
  12. ncbi request reprint Inducing and sensing protein--protein interactions in living cells by selective cross-linking
    Guillaume Lemercier
    ISIC Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Angew Chem Int Ed Engl 46:4281-4. 2007
  13. ncbi request reprint Searching for the protein targets of bioactive molecules
    Christopher Chidley
    Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne
    Chimia (Aarau) 65:720-4. 2011
    ..We discuss the advantages and limitations of the approach using the clinically approved drug sulfasalazine as an example...
  14. doi request reprint A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
    Grazvydas Lukinavicius
    Ecole Polytechnique Federale de Lausanne, Institute of Chemical Sciences and Engineering ISIC, National Centre of Competence in Research NCCR in Chemical Biology, 1015 Lausanne, Switzerland
    Nat Chem 5:132-9. 2013
    ..The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging...
  15. doi request reprint Visualizing biochemical activities in living cells through chemistry
    Luc Reymond
    Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chimia (Aarau) 65:868-71. 2011
    ..In this article, we review two of these labeling approaches, the SNAP-tag and CLIP-tag technologies, and their use for studying cellular processes...
  16. ncbi request reprint Caged substrates for protein labeling and immobilization
    Sambashiva Banala
    Ecole Polytechnique Federal de Lausanne EPFL, Institute of Chemical Sciences and Engineering, 1015 Lausanne, Switzerland
    Chembiochem 9:38-41. 2008
  17. ncbi request reprint Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo
    Alexandre Juillerat
    Institute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology, CH 1015 Lausanne, Switzerland
    Chem Biol 10:313-7. 2003
    ..In addition to various applications in proteomics, the selected mutants also yield insight into the interaction of the DNA repair protein hAGT with its inhibitor O(6)-benzylguanine...
  18. ncbi request reprint Induced protein dimerization in vivo through covalent labeling
    Susanne Gendreizig
    Institute of Molecular and Biological Chemistry, Institute of Biomolecular Sciences, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 125:14970-1. 2003
    ..The specificity of the labeling reaction and its irreversible nature should allow the approach to become a valuable tool to study and control protein dimerization in vivo...
  19. doi request reprint A fluorescent sensor for GABA and synthetic GABA(B) receptor ligands
    Anastasiya Masharina
    Institute of Chemical Sciences and Engineering ISIC, Institute of Bioengineering, NCCR in Chemical Biology, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    J Am Chem Soc 134:19026-34. 2012
    ..These properties make GABA-Snifit a valuable tool to investigate the role of GABA and GABA(B) in biological systems...
  20. ncbi request reprint Post-translational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells
    Michael Prummer
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Chembiochem 7:908-11. 2006
  21. doi request reprint Semisynthetic fluorescent sensor proteins based on self-labeling protein tags
    Matthias A Brun
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    J Am Chem Soc 131:5873-84. 2009
    ..These features should allow semisynthetic fluorescent sensor proteins based on SNAP-tag to become important tools for probing previously inaccessible metabolites...
  22. doi request reprint Fura-2FF-based calcium indicator for protein labeling
    Agostina A Ruggiu
    Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Org Biomol Chem 8:3398-401. 2010
    ..We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein...
  23. doi request reprint Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore
    Mako Kamiya
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    Anal Chem 82:6472-9. 2010
    ..The high sensitivity of the indicator together with the possibility to selectively couple it to proteins of interest makes it a powerful tool for measuring local changes in Ca2+ concentrations in living cells...
  24. ncbi request reprint Multicolor imaging of cell surface proteins
    Laura Vivero-Pol
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 127:12770-1. 2005
    ..In another application, fusions to different CPs can be selectively labeled with different fluorophores in one sample. Both applications open up new ways for studying the properties of cell surface proteins of living cells...
  25. ncbi request reprint Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells
    Alexandre Juillerat
    Ecole Polytechnique Federale de Lausanne, Institute of Chemical Sciences and Engineering, 1015 Lausanne, Switzerland
    Chembiochem 6:1263-9. 2005
    ....
  26. doi request reprint Switchable fluorophores for protein labeling in living cells
    Grazvydas Lukinavicius
    Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Curr Opin Chem Biol 15:768-74. 2011
    ....
  27. doi request reprint Semisynthesis of fluorescent metabolite sensors on cell surfaces
    Matthias A Brun
    Institute of Chemical Sciences and Engineering, National Centre of Competence in Research Chemical Biology, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Am Chem Soc 133:16235-42. 2011
    ..This work establishes a generally applicable and rational strategy for the generation of cell-surface-targeted fluorescent sensor proteins for metabolites of interest...
  28. pmc Labeling of fusion proteins with synthetic fluorophores in live cells
    Antje Keppler
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 101:9955-9. 2004
    ..The facile access to a variety of different AGT substrates as well as the specificity of the labeling reaction should make the approach an important tool to study protein function in live cells...
  29. doi request reprint A designed protein for the specific and covalent heteroconjugation of biomolecules
    Christopher Chidley
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, CH 1015 Lausanne, Switzerland
    Bioconjug Chem 19:1753-6. 2008
    ..The efficiency and specificity of covalin-based bioconjugations together with the availability of a large variety of substrates create immediate and ubiquitous applications for covalin in bioconjugate chemistry...
  30. pmc FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells
    Bruno H Meyer
    Ecole Polytechnique Federale de Lausanne EPFL, Institut des Sciences et Ingenierie Chimiques, CH 1015 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 103:2138-43. 2006
    ..The strong dependence of FRET on the receptor concentration further reveals that NK1Rs tend to concentrate in microdomains, which are found to constitute approximately 1% of the cell membrane and to be sensitive to cholesterol depletion...
  31. ncbi request reprint Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution
    Petra Tafelmeyer
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 11:681-9. 2004
    ..This powerful selection complements the repertoire of the currently used split-protein sensors and provides a new tool for high-throughput interaction screening...
  32. ncbi request reprint Specific labeling of cell surface proteins with chemically diverse compounds
    Nathalie George
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 126:8896-7. 2004
    ..The specificity and versatility of the labeling should allow it to become an important tool for studying and manipulating cell surface proteins and for complementing existing approaches in cell surface engineering...
  33. doi request reprint Tetrahydrobiopterin biosynthesis as an off-target of sulfa drugs
    Hirohito Haruki
    EPFL, Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research in Chemical Biology, 1015 Lausanne, Switzerland
    Science 340:987-91. 2013
    ..Our findings reveal an unexpected aspect of the pharmacology of sulfa drugs and might translate into their improved medical use...
  34. doi request reprint Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability
    Birgit Mollwitz
    Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Biochemistry 51:986-94. 2012
    ....
  35. doi request reprint Using peptide loop insertion mutagenesis for the evolution of proteins
    Christian Heinis
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de, Lausanne EPFL, Lausanne, Switzerland
    Methods Mol Biol 634:217-32. 2010
    ..The experimental procedures are general and can easily be adapted for the insertion of peptide loops into other classes of enzymes or into any protein of interest...
  36. ncbi request reprint Yeast three-hybrid screening for identifying anti-tuberculosis drug targets
    Simone Moser
    Institute of Chemical Science and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR Chemical Biology, Ecole Polytechnique Federale de Lausanne, EPFL SB ISIC LIP, 1015 Lausanne Switzerland Current address Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
    Chembiochem 14:2239-42. 2013
    ..The system employs libraries of randomly fragmented bacterial DNA and offers a technically simple alternative approach for target identification. ..
  37. ncbi request reprint Chemical probes shed light on protein function
    Kai Johnsson
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Curr Opin Struct Biol 17:488-94. 2007
    ..The past two years have seen significant advances in such methods, the publication of a number of new tags for labeling, and the imaginative application of established techniques to tackle previously intractable biological questions...
  38. doi request reprint A split-protein sensor for studying protein-protein interaction in mycobacteria
    Helen O'Hare
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    J Microbiol Methods 73:79-84. 2008
    ..Here we introduce Split-Trp to Escherichia coli and Mycobacterium smegmatis and demonstrate that this system is a simple assay for protein interaction in both organisms...
  39. ncbi request reprint Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins
    Maik Kindermann
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    Bioorg Med Chem Lett 14:2725-8. 2004
    ..These molecules should become useful tools for various applications in functional proteomics...
  40. pmc Indo-1 derivatives for local calcium sensing
    Michael Bannwarth
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
    ACS Chem Biol 4:179-190. 2009
    ....
  41. doi request reprint Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 centrosomal complex
    Grazvydas Lukinavicius
    Institute of Chemical Sciences and Engineering ISIC, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne 1015, Switzerland
    Curr Biol 23:265-70. 2013
    ..Our findings provide novel insights into protein interactions critical for centrosome biology and establish a toolbox for future studies of centrosomal proteins...
  42. doi request reprint Benzothiazinones are suicide inhibitors of mycobacterial decaprenylphosphoryl-β-D-ribofuranose 2'-oxidase DprE1
    Claudia Trefzer
    More Medicines for Tuberculosis MM4TB Consortium, and Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne EPFL, Lausanne, Switzerland
    J Am Chem Soc 134:912-5. 2012
    ..Our results explain the basis for drug sensitivity and resistance to an exceptionally potent class of antituberculosis agents...
  43. doi request reprint A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis
    Christopher Chidley
    Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research Chemical Biology, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Nat Chem Biol 7:375-83. 2011
    ..This work thus establishes a powerful approach for drug profiling and provides new insights in the mechanism of action of clinically approved drugs...
  44. doi request reprint Phosphopantetheinyl transferase-catalyzed formation of bioactive hydrogels for tissue engineering
    Katarzyna A Mosiewicz
    Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Am Chem Soc 132:5972-4. 2010
    ..3D encapsulation of cells resulted in high cell viability (ca. 95%) and single cell migration over long distances within RGDS-modified gels...
  45. doi request reprint Measuring in vivo protein half-life
    Karolina Bojkowska
    School of Life Sciences, Frontiers in Genetics National Program, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 18:805-15. 2011
    ..These results open broad perspectives for studying protein function in living animals...
  46. doi request reprint Subunit-specific surface mobility of differentially labeled AMPA receptor subunits
    Michel Kropf
    Brain Mind Institute, Faculté des Sciences de la Vie, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    Eur J Cell Biol 87:763-78. 2008
    ..These results indicate that surface mobility is regulated by different subunit compositions of AMPA receptors...
  47. pmc Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)
    Matthias Geissbuehler
    Laboratoire d Optique Biomedicale, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Biophys J 98:339-49. 2010
    ..The proposed method has the potential to become a new tool for investigating oxygen metabolism at the single cell and the subcellular level...
  48. ncbi request reprint Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring
    Rudolf Griss
    1 Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland 2 École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Lausanne, Switzerland 3 National Centre of Competence in Research in Chemical Biology, Lausanne, Switzerland
    Nat Chem Biol 10:598-603. 2014
    ..This low-cost point-of-care method could make therapies safer, increase the convenience of doctors and patients and make therapeutic drug monitoring available in regions with poor infrastructure. ..
  49. doi request reprint How to obtain labeled proteins and what to do with them
    Marlon J Hinner
    Institute of Chemical Sciences and Engineering, Laboratory of Protein Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Curr Opin Biotechnol 21:766-76. 2010
    ..We conclude with a view on necessary future developments, with one example being the selective labeling of non-tagged, native proteins in complex protein mixtures...
  50. ncbi request reprint Protein-functionalized polymer brushes
    Stefano Tugulu
    Laboratoire des Polymères, Institut des materiaux, Bâtiment MX D, and Laboratoire d Ingénierie des Protéines, Institut des Sciences et Ingenierie Chimiques, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Biomacromolecules 6:1602-7. 2005
    ..These protein-modified polymer brushes are attractive candidates for the development of protein microarrays...
  51. ncbi request reprint Adding value to fusion proteins through covalent labelling
    Thomas Gronemeyer
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, CH 1015 Lausanne, Switzerland
    Curr Opin Biotechnol 16:453-8. 2005
    ..Several new approaches have been developed for the covalent labelling of fusion proteins both in living cells and in vitro...
  52. ncbi request reprint A general method for the covalent labeling of fusion proteins with small molecules in vivo
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Nat Biotechnol 21:86-9. 2003
    ..Here we describe a general method for the covalent labeling of fusion proteins in vivo that complements existing methods for noncovalent labeling of proteins and that may open up new ways of studying proteins in living cells...
  53. ncbi request reprint Fluorogenic probes for live-cell imaging of the cytoskeleton
    Grazvydas Lukinavicius
    1 Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland 2
    Nat Methods 11:731-3. 2014
    ....
  54. ncbi request reprint Covalent and selective immobilization of fusion proteins
    Maik Kindermann
    Institute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 125:7810-1. 2003
    ....
  55. doi request reprint A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red
    Efthymia Prifti
    Ecole Polytechnique Federale de Lausanne EPFL, Institute of Chemical Sciences and Engineering, National Centre of Competence in Research NCCR in Chemical Biology, 1015 Lausanne, Switzerland
    ACS Chem Biol 9:606-12. 2014
    ..This work introduces a new approach for generating a powerful turn-on probe for "no-wash" labeling of plasma membrane proteins with numerous applications in bioimaging. ..
  56. doi request reprint Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma
    Karolina Bojkowska
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    Hepatology 56:1279-90. 2012
    ....
  57. ncbi request reprint Fast directed evolution of non-immunoglobulin proteins by somatic hypermutation in immune cells
    Christian Heinis
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Chembiochem 6:804-6. 2005
  58. ncbi request reprint A caged, localizable rhodamine derivative for superresolution microscopy
    Sambashiva Banala
    Institute of Chemical Sciences and Engineering and Laboratory of Experimental Biophysics, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    ACS Chem Biol 7:289-93. 2012
    ..The high contrast ratio is confirmed both in bulk and at the single molecule level. This property, together with its high photon yield makes it an excellent dye for photoactivated localization microscopy (PALM), as we demonstrate here...
  59. doi request reprint Benzothiazinones: prodrugs that covalently modify the decaprenylphosphoryl-β-D-ribose 2'-epimerase DprE1 of Mycobacterium tuberculosis
    Claudia Trefzer
    New Medicines for Tuberculosis Consortium, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    J Am Chem Soc 132:13663-5. 2010
    ..Here we demonstrate that BTZs are activated in the bacterium by reduction of an essential nitro group to a nitroso derivative, which then specifically reacts with a cysteine residue in the active site of DprE1...
  60. doi request reprint Regulation of glutamate metabolism by protein kinases in mycobacteria
    Helen M O'Hare
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Mol Microbiol 70:1408-23. 2008
    ..Previous reports of a defect in glutamate metabolism caused by pknG deletion may thus be explained by the effect of unphosphorylated GarA on these two enzyme activities, which may also contribute to the attenuation of virulence...
  61. doi request reprint Sensing acetylcholine and anticholinesterase compounds
    Alberto Schena
    Ecole Polytechnique Federale de Lausanne, Institute of Chemical Sciences and Engineering ISIC, Institute of Bioengineering, National Centre of Competence in Research NCCR in Chemical Biology, 1015 Lausanne Switzerland
    Angew Chem Int Ed Engl 53:1302-5. 2014
    ..The probe possesses good sensitivity, tunable detection range, and can be selectively targeted to cell surfaces, thereby making it an attractive tool for applications in analytical chemistry and quantitative biology. ..
  62. ncbi request reprint The laboratory in a droplet
    Helen M O'Hare
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Chem Biol 12:1255-7. 2005
    ..This novel methodology increases the throughput of a typical enzyme screen by two orders of magnitude...