M Molinari

Summary

Affiliation: Institute for Research in Biomedicine
Country: Switzerland

Publications

  1. ncbi request reprint The secretory capacity of a cell depends on the efficiency of endoplasmic reticulum-associated degradation
    M Molinari
    Institute for Research in Biomedicine, Via V Vela 6, 6500 Bellinzona, Switzerland
    Curr Top Microbiol Immunol 300:1-15. 2005
  2. ncbi request reprint Analyzing cotranslational protein folding and disulfide formation by diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Methods Enzymol 348:35-42. 2002
  3. ncbi request reprint EDEM1 regulates ER-associated degradation by accelerating de-mannosylation of folding-defective polypeptides and by inhibiting their covalent aggregation
    Silvia Olivari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Biochem Biophys Res Commun 349:1278-84. 2006
  4. ncbi request reprint N-glycan processing in ER quality control
    Lloyd W Ruddock
    Biocenter Oulu and Department of Biochemistry, University of Oulu, FIN 90014 Oulu, Finland
    J Cell Sci 119:4373-80. 2006
  5. ncbi request reprint Persistent glycoprotein misfolding activates the glucosidase II/UGT1-driven calnexin cycle to delay aggregation and loss of folding competence
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Mol Cell 20:503-12. 2005
  6. ncbi request reprint N-glycan structure dictates extension of protein folding or onset of disposal
    Maurizio Molinari
    Institute for Research in Biomedicine, Via V Vela 6, CH 6500 Bellinzona, Switzerland
    Nat Chem Biol 3:313-20. 2007
  7. ncbi request reprint Contrasting functions of calreticulin and calnexin in glycoprotein folding and ER quality control
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Mol Cell 13:125-35. 2004
  8. ncbi request reprint Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Science 299:1397-400. 2003
  9. pmc Sequential assistance of molecular chaperones and transient formation of covalent complexes during protein degradation from the ER
    Maurizio Molinari
    Institute for Research in Biomedicine, Via Vela 6, CH 6500 Bellinzona, Switzerland
    J Cell Biol 158:247-57. 2002
  10. ncbi request reprint Folding of viral glycoproteins in the endoplasmic reticulum
    Maurizio Molinari
    Institute of Biochemistry, ETH Zurich, Switzerland
    Virus Res 82:83-6. 2002

Collaborators

Detail Information

Publications26

  1. ncbi request reprint The secretory capacity of a cell depends on the efficiency of endoplasmic reticulum-associated degradation
    M Molinari
    Institute for Research in Biomedicine, Via V Vela 6, 6500 Bellinzona, Switzerland
    Curr Top Microbiol Immunol 300:1-15. 2005
    ..The capacity of ERAD also critically determines the efficiency of protein secretion. Here we summarize recent findings highlighting the role of ERAD in disease and development, particularly in professional secretory cells...
  2. ncbi request reprint Analyzing cotranslational protein folding and disulfide formation by diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Methods Enzymol 348:35-42. 2002
  3. ncbi request reprint EDEM1 regulates ER-associated degradation by accelerating de-mannosylation of folding-defective polypeptides and by inhibiting their covalent aggregation
    Silvia Olivari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Biochem Biophys Res Commun 349:1278-84. 2006
    ..This mutation did not affect the chaperone function of EDEM1...
  4. ncbi request reprint N-glycan processing in ER quality control
    Lloyd W Ruddock
    Biocenter Oulu and Department of Biochemistry, University of Oulu, FIN 90014 Oulu, Finland
    J Cell Sci 119:4373-80. 2006
    ..Tight regulation of N-glycan modifications is crucial to maintain protein quality control, to ensure the synthesis of functional polypeptides and to avoid constipation of the ER with folding-defective polypeptides...
  5. ncbi request reprint Persistent glycoprotein misfolding activates the glucosidase II/UGT1-driven calnexin cycle to delay aggregation and loss of folding competence
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Mol Cell 20:503-12. 2005
    ....
  6. ncbi request reprint N-glycan structure dictates extension of protein folding or onset of disposal
    Maurizio Molinari
    Institute for Research in Biomedicine, Via V Vela 6, CH 6500 Bellinzona, Switzerland
    Nat Chem Biol 3:313-20. 2007
    ..Their activity must be tightly regulated because the N-glycan composition determines whether the associated protein is subjected to folding attempts in the ER lumen or whether it is retrotranslocated into the cytosol and degraded...
  7. ncbi request reprint Contrasting functions of calreticulin and calnexin in glycoprotein folding and ER quality control
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Mol Cell 13:125-35. 2004
    ..Although not fully interchangeable during assistance of glycoprotein folding, calreticulin and calnexin may work, independently, as efficient and crucial factors for retention in the ER of nonnative polypeptides...
  8. ncbi request reprint Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle
    Maurizio Molinari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Science 299:1397-400. 2003
    ..Up-regulation of EDEM during ER stress may promote cell recovery by clearing the calnexin cycle and by accelerating ERAD of terminally misfolded polypeptides...
  9. pmc Sequential assistance of molecular chaperones and transient formation of covalent complexes during protein degradation from the ER
    Maurizio Molinari
    Institute for Research in Biomedicine, Via Vela 6, CH 6500 Bellinzona, Switzerland
    J Cell Biol 158:247-57. 2002
    ....
  10. ncbi request reprint Folding of viral glycoproteins in the endoplasmic reticulum
    Maurizio Molinari
    Institute of Biochemistry, ETH Zurich, Switzerland
    Virus Res 82:83-6. 2002
    ..g., cystic fibrosis and hereditary lung emphysema)...
  11. ncbi request reprint In and out of the ER: protein folding, quality control, degradation, and related human diseases
    Daniel N Hebert
    Department of Biochemistry and Molecular Biology, Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Physiol Rev 87:1377-408. 2007
    ....
  12. pmc A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal
    Riccardo Bernasconi
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    J Biol Chem 283:16446-54. 2008
    ..Thus, OS-9.1 and OS-9.2 play a dual role in mammalian ER quality control: first as crucial retention factors for misfolded conformers, and second as promoters of protein disposal from the ER lumen...
  13. ncbi request reprint Glycoprotein folding and the role of EDEM1, EDEM2 and EDEM3 in degradation of folding-defective glycoproteins
    Silvia Olivari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    FEBS Lett 581:3658-64. 2007
    ..This review describes current knowledge about mechanisms regulating folding and disposal of glycoproteins...
  14. ncbi request reprint Microbiology: death of a chaperone
    Cesare Montecucco
    Nature 443:511-2. 2006
  15. ncbi request reprint Substrate-specific requirements for UGT1-dependent release from calnexin
    Tatiana Soldà
    Institute for Research in Biomedicine, Bellinzona, Switzerland
    Mol Cell 27:238-49. 2007
    ..For other proteins, UGT1 deletion substantially delays release from calnexin, unexpectedly showing that UGT1 activity might be required for a structural maturation needed for substrate dissociation from calnexin and export from the ER...
  16. ncbi request reprint Consequences of ERp57 deletion on oxidative folding of obligate and facultative clients of the calnexin cycle
    Tatiana Soldà
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    J Biol Chem 281:6219-26. 2006
    ..ERp72 was identified as one of the ER-resident oxidoreductases associating with the orphan ERp57 substrates to maintain their folding competence...
  17. ncbi request reprint The disulphide bonds in the catalytic domain of BACE are critical but not essential for amyloid precursor protein processing activity
    Frauke Fischer
    Nervous System, Novartis Pharma AG, Basel, Switzerland Institute for Research in Biomedicine, Bellinzona, Switzerland
    J Neurochem 80:1079-88. 2002
    ....
  18. pmc Early postnatal death and motor disorders in mice congenitally deficient in calnexin expression
    Angela Denzel
    Imperial Cancer Research Fund, Academic Department of Histopathology, Bart s and the Queen Mary s School of Medicine and Dentistry, Whitechapel, United Kingdom
    Mol Cell Biol 22:7398-404. 2002
    ..Thus, the critical contribution of calnexin to mammalian physiology is tissue specific...
  19. ncbi request reprint EDEM contributes to maintenance of protein folding efficiency and secretory capacity
    Klara K Eriksson
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    J Biol Chem 279:44600-5. 2004
    ..Our data underscore the important role that the degradation machinery plays in maintaining a functional folding environment in the ER...
  20. ncbi request reprint A novel stress-induced EDEM variant regulating endoplasmic reticulum-associated glycoprotein degradation
    Silvia Olivari
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    J Biol Chem 280:2424-8. 2005
    ....
  21. ncbi request reprint Degradation of trafficking-defective long QT syndrome type II mutant channels by the ubiquitin-proteasome pathway
    Qiuming Gong
    Division of Molecular Medicine, Department of Medicine, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Biol Chem 280:19419-25. 2005
    ..These results suggest that the degradation of LQT2 mutant channels is mediated by the cytosolic proteasome in a process that involves mannose trimming, polyubiquitination, and deglycosylation of mutant channels...
  22. pmc beta-site specific intrabodies to decrease and prevent generation of Alzheimer's Abeta peptide
    Paolo Paganetti
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    J Cell Biol 168:863-8. 2005
    ....
  23. ncbi request reprint The glycan code of the endoplasmic reticulum: asparagine-linked carbohydrates as protein maturation and quality-control tags
    Daniel N Hebert
    Department of Biochemistry and Molecular Biology, Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Trends Cell Biol 15:364-70. 2005
    ..These glycosidases and transferases work in concert with resident secretory pathway carbohydrate-binding proteins to form a network that assists in the maturation and trafficking of both native and aberrant glycoproteins within the cell...
  24. ncbi request reprint The use of calnexin and calreticulin by cellular and viral glycoproteins
    Michel Pieren
    Institute for Research in Biomedicine, 6500 Bellinzona, Switzerland
    J Biol Chem 280:28265-71. 2005
    ..This may explain why inactivation of the calnexin cycle affects viral replication and infectivity but not viability of mammalian cells...
  25. doi request reprint Segregation and rapid turnover of EDEM1 by an autophagy-like mechanism modulates standard ERAD and folding activities
    Tito Cali
    Institute for Research in Biomedicine, Protein Folding and Quality Control, Via V Vela 6, CH 6500 Bellinzona, Switzerland
    Biochem Biophys Res Commun 371:405-10. 2008
    ..We define as ERAD tuning the mechanisms operating in the mammalian ER at steady state to offer kinetic advantage to folding over disposal of unstructured nascent chains by selective and rapid degradation of ERAD regulators...
  26. ncbi request reprint N-linked glycan recognition and processing: the molecular basis of endoplasmic reticulum quality control
    Kelley W Moremen
    Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602 4712, USA
    Curr Opin Struct Biol 16:592-9. 2006
    ..Additionally, dismantling of the polypeptide-bound N-glycan interrupts futile folding attempts, and elicits preparation of the misfolded chain for dislocation into the cytosol and degradation...