PIERRE GONCZY

Summary

Country: Switzerland

Publications

  1. doi request reprint Mechanisms of asymmetric cell division: flies and worms pave the way
    PIERRE GONCZY
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
    Nat Rev Mol Cell Biol 9:355-66. 2008
  2. doi request reprint Towards a molecular architecture of centriole assembly
    PIERRE GONCZY
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL Lausanne, Switzerland
    Nat Rev Mol Cell Biol 13:425-35. 2012
  3. ncbi request reprint RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryos
    Katayoun Afshar
    Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges Lausanne, Switzerland
    Cell 119:219-30. 2004
  4. ncbi request reprint ZYG-9, TAC-1 and ZYG-8 together ensure correct microtubule function throughout the cell cycle of C. elegans embryos
    Jean Michel Bellanger
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
    J Cell Sci 120:2963-73. 2007
  5. ncbi request reprint SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells
    Sebastian Leidel
    Swiss Institute for Experimental Cancer Research ISREC, CH 1066 Epalinges Lausanne, Switzerland
    Nat Cell Biol 7:115-25. 2005
  6. doi request reprint Discovering regulators of centriole biogenesis through siRNA-based functional genomics in human cells
    Fernando R Balestra
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, 1015 Lausanne, Switzerland
    Dev Cell 25:555-71. 2013
  7. pmc The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos
    Virginie Hachet
    Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    Mol Biol Cell 23:3111-21. 2012
  8. doi request reprint Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL
    Daiju Kitagawa
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    J Cell Sci 124:3884-93. 2011
  9. pmc Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle
    Petr Strnad
    Swiss Institute for Experimental Cancer Research, Swiss Federal Institute of Technology, School of Life Sciences, CH 1066 Lausanne, Switzerland
    Dev Cell 13:203-13. 2007
  10. ncbi request reprint Coupling of cortical dynein and G alpha proteins mediates spindle positioning in Caenorhabditis elegans
    Tu Nguyen-Ngoc
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland CH 1066
    Nat Cell Biol 9:1294-302. 2007

Collaborators

  • Suliana Manley
  • Francis S Willard
  • DAVID SIDEROVSKI
  • Alexey L Khodjakov
  • Mette M Mogensen
  • Asako Sugimoto
  • Takashi Ishikawa
  • Michael Glotzer
  • Ioannis Vakonakis
  • Jadranka Loncarek
  • Felix Naef
  • Ralf Schnabel
  • Stephan W Grill
  • B F McEwen
  • BRUCE A BOWERMAN
  • Brian D Dynlacht
  • Daiju Kitagawa
  • Petr Strnad
  • Isabelle Flückiger
  • Virginie Hachet
  • Debora Keller
  • Katayoun Afshar
  • Yemima Budirahardja
  • Nicolas Olivier
  • Sachin Kotak
  • Paul Guichard
  • Sebastian Leidel
  • Coralie Busso
  • Marie Delattre
  • Fernando R Balestra
  • Jean Michel Bellanger
  • Gregor Kohlmaier
  • Michel O Steinmetz
  • Tamara Mikeladze-Dvali
  • Natacha Olieric
  • Karine Baumer
  • Kalyani Thyagarajan
  • Kelly Colombo
  • Simon Blanchoud
  • Alexandra Bezler
  • Tu Nguyen-Ngoc
  • Coralie Canard
  • Moira M Cockell
  • Akinori Yamada
  • Aitana Neves
  • Davide Demurtas
  • Norbert Majubu
  • Vinoth Sundar Rajan
  • Yuichi Hongoh
  • Kumiko Kihara
  • Yuichiro Nishida
  • Shigeharu Moriya
  • Graham Knott
  • Heinrich Leonhardt
  • Peter Askjaer
  • Andreas Brune
  • Lothar Schermelleh
  • Mika Toya
  • Carsten Dietrich
  • Carsten Sachse
  • Ambroise Desfosses
  • Lukas von Tobel
  • Aditi Maheshwari
  • Vincent Olieric
  • Miriam Bortfeld
  • Jolanta Polanowska
  • Manuel Hilbert
  • Michele C Erat
  • Jerome Reboul
  • Michael E Werner
  • Yu Chung Tse
  • Xing Meng
  • Alexander Spektor
  • Ursula Euteneuer
  • J Clayton Carter
  • Tatiana Vinogradova
  • Jennifer B Phillips
  • Lorenzo Cerutti
  • Khursheed Wani
  • Christopher R McCudden
  • Heinke Schnabel
  • Jeannine Bamat
  • Richard Feichtinger
  • Christopher A Johnston
  • Randall J Kimple

Detail Information

Publications36

  1. doi request reprint Mechanisms of asymmetric cell division: flies and worms pave the way
    PIERRE GONCZY
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
    Nat Rev Mol Cell Biol 9:355-66. 2008
    ..Lessons learned from these two model organisms also apply to cells that divide asymmetrically in other metazoans, such as self-renewing stem cells in mammals...
  2. doi request reprint Towards a molecular architecture of centriole assembly
    PIERRE GONCZY
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL Lausanne, Switzerland
    Nat Rev Mol Cell Biol 13:425-35. 2012
    ..These advances have brought the field to an exciting era in which architecture meets function...
  3. ncbi request reprint RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryos
    Katayoun Afshar
    Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges Lausanne, Switzerland
    Cell 119:219-30. 2004
    ..Our findings suggest a mechanism in which RIC-8 favors generation of Galpha free from Gbetagamma and enables GPR-1/2 to mediate asymmetric cell division...
  4. ncbi request reprint ZYG-9, TAC-1 and ZYG-8 together ensure correct microtubule function throughout the cell cycle of C. elegans embryos
    Jean Michel Bellanger
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
    J Cell Sci 120:2963-73. 2007
    ..Taken together, these findings indicate that ZYG-9-TAC-1 and ZYG-8 act in a partially redundant manner to ensure correct microtubule assembly throughout the cell cycle of early C. elegans embryos...
  5. ncbi request reprint SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells
    Sebastian Leidel
    Swiss Institute for Experimental Cancer Research ISREC, CH 1066 Epalinges Lausanne, Switzerland
    Nat Cell Biol 7:115-25. 2005
    ..Therefore, HsSAS-6 is also required for centrosome duplication, indicating that the function of SAS-6-related proteins has been widely conserved during evolution...
  6. doi request reprint Discovering regulators of centriole biogenesis through siRNA-based functional genomics in human cells
    Fernando R Balestra
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, 1015 Lausanne, Switzerland
    Dev Cell 25:555-71. 2013
    ..We developed a dynamic web interface containing all images and numerical features as a powerful resource to investigate facets of centrosome biology. ..
  7. pmc The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos
    Virginie Hachet
    Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    Mol Biol Cell 23:3111-21. 2012
    ..Our findings taken together support a model in which centrosomes and AIR-1 promote timely onset of mitosis by locally removing NPP-3 and associated nucleoporins from the NE...
  8. doi request reprint Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL
    Daiju Kitagawa
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    J Cell Sci 124:3884-93. 2011
    ..Our findings are compatible with the notion that mutations in CPAP and STIL cause MCPH because of aberrant spindle positioning in progenitor cells during brain development...
  9. pmc Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle
    Petr Strnad
    Swiss Institute for Experimental Cancer Research, Swiss Federal Institute of Technology, School of Life Sciences, CH 1066 Lausanne, Switzerland
    Dev Cell 13:203-13. 2007
    ....
  10. ncbi request reprint Coupling of cortical dynein and G alpha proteins mediates spindle positioning in Caenorhabditis elegans
    Tu Nguyen-Ngoc
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland CH 1066
    Nat Cell Biol 9:1294-302. 2007
    ..Together with microtubule dynamics, this allows pulling forces to be exerted and proper cell division to be achieved...
  11. pmc Polarity mediates asymmetric trafficking of the Gbeta heterotrimeric G-protein subunit GPB-1 in C. elegans embryos
    Kalyani Thyagarajan
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne CH 1015, Switzerland
    Development 138:2773-82. 2011
    ..Overall, our results lead us to propose that modulation of Gβ trafficking plays a crucial role during the asymmetric division of one-cell stage C. elegans embryos...
  12. doi request reprint PLK-1 asymmetry contributes to asynchronous cell division of C. elegans embryos
    Yemima Budirahardja
    Swiss Institute for Experimental Cancer Research ISREC Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    Development 135:1303-13. 2008
    ....
  13. ncbi request reprint Centriolar SAS-5 is required for centrosome duplication in C. elegans
    Marie Delattre
    Swiss Institute for Experimental Cancer Research ISREC, CH 1066 Epalinges Lausanne, Switzerland
    Nat Cell Biol 6:656-64. 2004
    ..Furthermore, partial RNA-interference (RNAi)-mediated inactivation experiments suggest that both sas-5 and zyg-1 are dose-dependent regulators of centrosome duplication...
  14. pmc Structural basis of the 9-fold symmetry of centrioles
    Daiju Kitagawa
    Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology, EPFL, Lausanne, Switzerland
    Cell 144:364-75. 2011
    ..Overall, our findings establish a structural basis for the universal 9-fold symmetry of centrioles...
  15. pmc Overly long centrioles and defective cell division upon excess of the SAS-4-related protein CPAP
    Gregor Kohlmaier
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    Curr Biol 19:1012-8. 2009
    ..This in turn leads to multipolar spindle assembly and cytokinesis defects. Overall, our findings suggest that centriole length must be carefully regulated to restrict procentriole number and thus ensure accurate cell division...
  16. doi request reprint PP2A phosphatase acts upon SAS-5 to ensure centriole formation in C. elegans embryos
    Daiju Kitagawa
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, CH 1015 Lausanne, Switzerland
    Dev Cell 20:550-62. 2011
    ..These findings lead us to propose that PP2A-mediated loading of SAS-6 proteins is critical at the onset of centriole formation...
  17. doi request reprint Phosphorylation of SAS-6 by ZYG-1 is critical for centriole formation in C. elegans embryos
    Daiju Kitagawa
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, CH 1015 Lausanne, Switzerland
    Dev Cell 17:900-7. 2009
    ..Overall, our findings establish that phosphorylation of the evolutionarily conserved protein SAS-6 is critical for centriole formation and thus for faithful cell division...
  18. ncbi request reprint Centrosomes promote timely mitotic entry in C. elegans embryos
    Virginie Hachet
    Swiss Institute for Experimental Cancer Research ISREC, and School of Life Sciences, Swiss Federal Institute of Technology EPFL, CH 1066 Lausanne, Switzerland
    Dev Cell 12:531-41. 2007
    ..Together, our findings support a model in which centrosomes serve as integrative centers for mitotic regulators and thus trigger mitotic entry in a timely fashion...
  19. pmc Mutual antagonism between the anaphase promoting complex and the spindle assembly checkpoint contributes to mitotic timing in Caenorhabditis elegans
    Alexandra Bezler
    Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology, CH 1015 Lausanne, Switzerland
    Genetics 186:1271-83. 2010
    ..Taken together, our findings raise the possibility that the APC/C negatively regulates the SAC and, therefore, that the SAC and the APC/C have a mutual antagonistic relationship in C. elegans embryos...
  20. ncbi request reprint lis-1 is required for dynein-dependent cell division processes in C. elegans embryos
    Moira M Cockell
    Swiss Institute for Experimental Cancer Research ISREC, Ch des Boveresses 155, 1066 Epalinges Lausanne, Switzerland
    J Cell Sci 117:4571-82. 2004
    ..These results suggest that dynein and Lis1, albeit functioning in identical processes, are targeted partially independently of one another...
  21. ncbi request reprint Translation of polarity cues into asymmetric spindle positioning in Caenorhabditis elegans embryos
    Kelly Colombo
    Swiss Institute for Experimental Cancer Research ISREC, 1066 Epalinges Lausanne, Switzerland
    Science 300:1957-61. 2003
    ..Thus, the extent of net pulling forces may depend on cortical Galpha activity, which is regulated by anterior-posterior polarity cues through GPR-1/2...
  22. ncbi request reprint The arithmetic of centrosome biogenesis
    Marie Delattre
    Swiss Institute for Experimental Cancer Research ISREC, CH 1066 Epalinges Lausanne, Switzerland
    J Cell Sci 117:1619-30. 2004
    ..This occurs, for instance, in the early mouse embryo and in parthenogenetic species and might rely on a pre-existing seed that resides within PCM but is not visible by ultrastructural analysis...
  23. doi request reprint Analysis of centriole elimination during C. elegans oogenesis
    Tamara Mikeladze-Dvali
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL Lausanne, 1015 Switzerland
    Development 139:1670-9. 2012
    ..These findings set the stage for a mechanistic dissection of centriole elimination in a metazoan organism...
  24. ncbi request reprint SAS-4 is essential for centrosome duplication in C elegans and is recruited to daughter centrioles once per cell cycle
    Sebastian Leidel
    Swiss Institute for Experimental, Cancer Research ISREC, CH 1066 Epalinges, Lausanne, Switzerland
    Dev Cell 4:431-9. 2003
    ..Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle...
  25. doi request reprint Mechanisms of procentriole formation
    Petr Strnad
    Swiss Institute for Experimental Cancer Research ISREC, Swiss Federal Institute of Technology EPFL, School of Life Sciences, Lausanne, Switzerland
    Trends Cell Biol 18:389-96. 2008
    ..Here, we review recent findings that provide important mechanistic insights into how a single procentriole forms next to each centriole once per cell cycle, thus ensuring that one centrosome becomes two...
  26. ncbi request reprint TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos
    Jean Michel Bellanger
    Swiss Institute for Experimental Cancer Research ISREC, CH 1066, Epalinges Lausanne, Switzerland
    Curr Biol 13:1488-98. 2003
    ..While a large body of work has made important contributions to our understanding of the mechanisms governing microtubule dynamics in vitro, much remains to be learned about how these mechanisms operate in vivo...
  27. pmc Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos
    Katayoun Afshar
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne CH 1015, Switzerland
    Development 137:237-47. 2010
    ..Our findings provide the first insights into the role of a member of the PP6 family of phosphatases in metazoan development...
  28. pmc Cortical dynein is critical for proper spindle positioning in human cells
    Sachin Kotak
    Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology Lausanne, Lausanne CH 1015, Switzerland
    J Cell Biol 199:97-110. 2012
    ..Overall, we propose a model in which the ternary complex serves to anchor dynein at the plasma membrane to ensure correct spindle positioning...
  29. pmc Resolution doubling in 3D-STORM imaging through improved buffers
    Nicolas Olivier
    Laboratory for Experimental Biophysics, School of Basic Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    PLoS ONE 8:e69004. 2013
    ..Using this buffer to perform 3D-STORM on biological samples, we obtained images with better than 10 nanometer lateral and 30 nanometer axial resolution. ..
  30. doi request reprint Native architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry
    Paul Guichard
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, 1015 Lausanne, Switzerland
    Curr Biol 23:1620-8. 2013
    ..More generally, a high-resolution view of the proximal region of the centriole is lacking, thus limiting understanding of the underlying assembly mechanisms...
  31. doi request reprint Coupling the cell cycle to development
    Yemima Budirahardja
    Swiss Institute for Experimental Cancer Research ISREC Sciences, Swiss Federal Institute of Technology, Lausanne, Switzerland
    Development 136:2861-72. 2009
    ....
  32. pmc NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function
    Sachin Kotak
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    EMBO J 32:2517-29. 2013
    ..Our findings uncover a mechanism whereby the status of NuMA phosphorylation coordinates mitotic progression with proper spindle function. ..
  33. doi request reprint Cartwheel architecture of Trichonympha basal body
    Paul Guichard
    Swiss Institute for Experimental Cancer Research, School of Life Sciences, Swiss Federal Institute of Technology, Lausanne, Switzerland
    Science 337:553. 2012
    ..Thus, by using the power of biodiversity, we unveiled the architecture of the cartwheel at the root of the ninefold symmetry of centrioles and basal bodies...
  34. ncbi request reprint Mechanisms of spindle positioning: focus on flies and worms
    PIERRE GONCZY
    Swiss Institute for Experimental Cancer Research ISREC, 155 ch des Boveresses, CH 1066 Epalinges Lausanne, Switzerland
    Trends Cell Biol 12:332-9. 2002
    ..This article discusses recent findings that indicate how this mechanism might be used for spindle positioning during Drosophila and Caenorhabditis elegans development...
  35. doi request reprint ASSET: a robust algorithm for the automated segmentation and standardization of early Caenorhabditis elegans embryos
    Simon Blanchoud
    Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Swiss Federal Institute of Technology EPFL, Lausanne, Switzerland
    Dev Dyn 239:3285-96. 2010
    ..In summary, we establish ASSET as a novel tool for the efficient quantification and standardization of images from early C. elegans embryos...
  36. pmc Simple buffers for 3D STORM microscopy
    Nicolas Olivier
    Laboratory for Experimental Biophysics, School of Basic Sciences, Swiss Federal Institute of Technology EPFL, CH 1015 Lausanne, Switzerland
    Biomed Opt Express 4:885-99. 2013
    ..We expect that this advance will promote the versatile utilization of 3D STORM by removing one of its entry barriers, as well as provide a more reproducible way to compare optical setups and data processing algorithms...